Methods for Identifying Risk of Low Bmd and Treatments Thereof

ABSTRACT

Provided herein are methods for identifying a risk of low BMD in a subject, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating low BMD-related disorders, such as osteoporosis, and therapeutic and preventative methods applicable to osteoporosis. These embodiments are based upon an analysis of polymorphic variations in nucleotide sequences within the human genome.

RELATED APPLICATIONS

This patent application claims the benefit of U.S. Provisional PatentApplication No. 60/640,966 filed on 31 Dec. 2004, entitled “Methods ForIdentifying Risk Of Low BMD And Treatments Thereof,” naming Steven Mahet al. as inventors, and designated by attorney docket no. SEQ-4095-PV.This patent application is related to U.S. Provisional PatentApplication Nos. 60/640,967 and 60/640,796, each filed on 31 Dec. 2004,entitled “Methods For Identifying Risk Of Low BMD And TreatmentsThereof,” naming Steven Mah et al. as inventors, and designated byattorney docket nos. SEQ-4094-PV and SEQ-4096-PV, respectively. Thecontent and subject matter of each of these patent applications ishereby incorporated by reference in its entirety, including all text anddrawings, in jurisdictions providing for such incorporation.

FIELD OF THE INVENTION

The invention relates to genetic methods for identifying susceptibilityto low bone mineral density (BMD) and/or bone damage generallyassociated with human diseases, and in particular to osteoporosis, andtreatments that specifically target the disease.

BACKGROUND

Osteoporosis is a common disease characterized by low bone mineraldensity (BMD), deterioration of bone micro-architecture and increasedrisk of bone damage, such as fracture. Common types of osteoporosisinclude postmenopausal and senile osteoporosis, which generally occurlater in life, e.g., 70+ years.

Osteoporosis is a major public health problem which affects quality oflife and increases costs to health care providers. It is estimated that44 million Americans and 100 million people worldwide are at risk forosteoporosis. In the United States today, 10 million individuals areestimated to already have the disease and almost 34 million more, or 55%of the people 50 years of age and older, have low bone mass, which putsthem at increased risk of developing osteoporosis and related fractures.Of the 10 million Americans estimated to have osteoporosis, eightmillion are women and 2 million are men. These numbers are growing asthe elderly population increases. It is estimated that by the middle ofthe next century the number of osteoporosis sufferers will double in theWest, but may increase six-fold in Asia and South America. The estimatednational direct expenditures (e.g., hospitals and nursing homes) forosteoporotic and associated fractures was $17 billion in 2001 ($47million each day)—and the cost is rising (See National OsteoporosisFoundation; http://www.nof.org/osteoporosis/stats.htm).

Fracture is the most serious endpoint of osteoporosis, particularlyfracture of the hip which affects up to 1.7 million people worldwideeach year. One in two women and one in four men over age 50 will have anosteoporosis-related fracture in their lifetime. It is estimated that bythe year 2050, the number of hip fractures worldwide will increase toover 6 million, as life expectancy and age of the population increase(See Spangler et al. “The Genetic Component of OsteoporosisMini-review”; http:www.csa.com.osteointro.html).

Peak bone mass is mainly genetically determined, though dietary factorsand physical activity can have positive effects. Peak bone mass occurswhen skeletal growth ceases, after which time bone loss starts. Incontrast to the positive balance that occurs during growth, inosteoporosis, the resorbed cavity is not completely refilled by bone andBMD decreases. Based on studies of family histories, twin studies, andracial factors, some attribute 50-60% of total bone variation (e.g.,Bone Mineral Density to genetic effects and suggest there may be apredisposition for osteoporosis.

Osteoporosis can be considered a complex genetic trait with variants ofseveral genes underlying the genetic determination of the variability ofthe phenotype. Low BMD is an important risk factor for fractures, theclinically most relevant feature of osteoporosis. Segregation analysisin families has shown that BMD is under polygenic control. In addition,biochemical markers of bone turnover have shown to have strong geneticcomponents. Several candidate genes have been analyzed in relation toBMD, but the most widely studied gene in this respect, the vitamin Dreceptor (VDR) gene, explains only a small part of the genetic effect onBMD. Numerous studies, focusing on the BsmI allele of the vitamin Dreceptor gene have concluded that absence of the restriction sitecorrelates with low bone mineral density.

SUMMARY

It has been discovered that certain polymorphic variations in humangenomic DNA are associated with the occurrence of low bone mineraldensity (BMD) and/or bone damage generally associated with humandiseases, and in particular to osteoporosis. In particular, polymorphicvariants in loci containing CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1/TNIP1 regions in human genomic DNA have been associated withrisk of low BMD.

Thus, featured herein are methods for identifying a subject at risk oflow bone mineral density (BMD) and/or bone fracture, which indicatesbone damage and related conditions such as osteoporosis in a subject.The methods comprise detecting the presence or absence of one or more ofthe polymorphic variations described herein in a human nucleic acidsample. In an embodiment, two or more polymorphic variations aredetected and in some embodiments, 3 or more, or 4, 5, 6, 7, 8, 9, 10,11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 or more polymorphic variantsare detected.

Also featured are nucleic acids that include one or more polymorphicvariations associated with occurrence of low BMD, as well aspolypeptides encoded by these nucleic acids. In addition, provided aremethods for identifying candidate therapeutic molecules for osteoporosisand other low BMD-related disorders, as well as methods for treatingosteoporosis in a subject by identifying a subject at risk of low BMDand treating the subject with a suitable prophylactic, treatment ortherapeutic molecule.

Also provided are compositions comprising a cell from a subjectsuffering from low BMD or at risk of low BMD, and a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1/TNIP1 nucleic acid, with a RNAi, siRNA, antisenseDNA or RNA, or ribozyme nucleic acid designed from a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleotide sequence, or a nucleic acid thathybridizes to such a nucleotide sequence under stringent conditions. Inan embodiment, the RNAi, siRNA, antisense DNA or RNA, or ribozymenucleic acid is designed from a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleotide sequence that includes one or more low BMD associatedpolymorphic variations, and in some instances, specifically interactswith such a nucleotide sequence. Further, provided are arrays of nucleicacids bound to a solid surface, in which one or more nucleic acidmolecules of the array have a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleotide sequence, or a fragment or substantially identical nucleicacid thereof, or a complementary nucleic acid of the foregoing. Featuredalso are compositions comprising a cell from a subject having low BMD orat risk of low BMD and/or a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1polypeptide, with an antibody that specifically binds to thepolypeptide. In an embodiment, the antibody specifically binds to anepitope in the polypeptide that includes a non-synonymous amino acidmodification associated with low BMD (e.g., results in an amino acidsubstitution in the encoded polypeptide associated with low BMD). In anembodiment, the antibody specifically binds to an epitope comprising anarginine corresponding to position 120 in a PROL4 polypeptide (SEQ IDNO: 12).

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1-4 show the position of each SNP in the chromosome on the x-axis,while the y-axis provides the negative logarithm of the p-valuecomparing the estimated allele frequency in the cases to that of thecontrol group. Also shown in the figures are exons and introns of thegenes in approximate chromosomal positions. More specifically, FIG. 1shows proximal SNPs in a CETP region in genomic DNA. FIG. 2 showsproximal SNPs in a PROW region in genomic DNA. FIG. 3 shows proximalSNPs in a GRID2 region in genomic DNA. FIG. 4 shows proximal SNPs in aPDE4D region in genomic DNA. FIG. 5 shows proximal SNPs in a GPX3/TNIP1region in genomic DNA.

DETAILED DESCRIPTION

It has been discovered that polymorphic variants described in a CETP,PROL4, GRID2, PDE4D and GPX3/TNIP1 loci in human genomic DNA areassociated with occurrence of low BMD in subjects. Thus, detectinggenetic determinants in and around this locus associated with anincreased risk of low BMD occurrence can lead to early identification ofa risk of low BMD, or its associated disorders such as osteoporosis, andearly application of preventative and treatment measures. Associatingthe polymorphic variants with low BMD also has provided new targets fordiagnosing low BMD, for prognosing osteoporosis, and methods forscreening molecules useful in osteoporosis treatments and osteoporosispreventatives.

Cholestery ester transfer protein (CETP) transfers cholesteryl estersbetween lipoproteins. The transfer of insoluble cholesteryl esters amonglipoprotein particles (HDL to LDL) by CETP is a step in normalcholesterol homeostasis. The lipoprotein phenotype of CETP deficiency,which is characterized by increased levels of HDL and decreased levelsof low density lipoprotein (LDL), appears to have antiatherogenicpotential. Bone mineral density is reduced by atherogenic diets(increased LDL/HDL ratios), possibly as a result of a shift in thebalance of bone marrow stromal cells away from osteoblasts and towardsadipocytes, which could result in reduced bone synthesis duringremodeling. Lipoproteins may directly regulate bone density via theLRP5, the low-density lipoprotein receptor related protein 5. LRP5 wascloned from an osteoblast cDNA library (Dong, et al.: Biochem BiophysRes Commun. 1998 Oct. 29; 251(3):784-90) and mouse knockouts show areduced BMD phenotype (Kato et. al.: J Cell Biol. 2002 Apr. 15;157(2):303-14). A G171V mutation in LRP5 resulted in increased numbersof osteoblasts, increased AP activity, and increased trabecular numberand thickness in mice (Babij et al.: J Bone Miner Res. 2003 June; 18(6):960-74). The human G171V mutation was found to have the same phenotype,presumably by increasing WNT signaling in osteoblasts (Mao et. al.: MolCell. 2001 April; 7(4):801-9). Overall, there is evidence thatlipoprotein profiles play a role in osteoblast differentiation. Providedis a method for treating osteoporosis or low bone mineral density bymodulating a CETP function in a subject suffering from osteoporosis orlow bone mineral density. CETP inhibitors and methods of making them aredescribed in U.S. Pat. No. 6,586,613 (Substituted tetrahydronaphthalineand analogous compounds); U.S. Pat. No. 6,562,976(4-phenyltetrahydrochinoline utilized as an inhibitor of the cholesterolester transfer protein); U.S. Pat. No. 6,387,929(4-heteroaryl-tetrahydroquinolines and their use as inhibitors of thecholesterin-ester transfer protein); U.S. Pat. No. 6,291,477(Tetrahydroquinolines, processes for their preparation, pharmaceuticalcompositions containing them, and their use to prevent or treathyperlipoproteinaemia); U.S. Pat. No. 6,218,431 (Substituted biphenyls);U.S. Pat. No. 6,207,671 (Cycloalkano-pyridines); U.S. Pat. No. 6,127,383(2-aryl-substituted pyridines); U.S. Pat. No. 6,121,330 (5-Hydroxyalkylsubstituted phenyls and their use in medicaments for the treatment ofarteriosclerosis and hyperlipoproteinaemia); U.S. Pat. No. 6,069,148(Cycloalkano-pyridines); U.S. Pat. No. 6,063,788 (Bicyclic-fusedpyridines); U.S. Pat. No. 5,932,587 (Heterocyclic-fused pyridines); U.S.Pat. No. 5,925,645 (2-aryl-substituted pyridines); U.S. Pat. No.6,753,346 (CETP activity inhibitor); U.S. Pat. No. 6,706,881 (Methodsfor preparing CETP inhibitors); U.S. Pat. No. 6,689,897 (Intermediatesof CETP inhibitors); U.S. Pat. No. 6,600,045 (Methods for preparing CETPinhibitors); U.S. Pat. No. 6,573,383 (Preparation of anhydrous CETPinhibitor); U.S. Pat. No. 6,555,113 (Modulation of cholesteryl estertransfer protein (CETP) activity); U.S. Pat. No. 6,426,365 (CETPactivity inhibitors); U.S. Pat. No. 6,410,022 (Modulation of cholesterylester transfer protein (CETP) activity); U.S. Pat. No. 6,410,020(Monoclonal antibody reactive to human CETP and assay method for humanCETP); U.S. Pat. No. 6,140,474 (Monoclonal antibody reactive withhuman-origin CETP and method of quantifying human-origin CETP); U.S.Pat. No. 5,948,435 (Methods of regulating CETP genes, enzymes and othercompound, and pharmaceutical composition therefor); U.S. Pat. No.5,519,001 (CETP inhibitor polypeptide antibodies against the syntheticpolypeptide and prophylactic and therapeutic anti-atherosclerosistreatments); and U.S. Pat. No. 5,512,548 (CETP inhibitor polypeptide,antibodies against the synthetic polypeptide and prophylactic andtherapeutic anti-atherosclerosis treatments).

PROL4, also known as Lacrimal proline rich protein (LPRP) is a member ofthe proline-rich secreted protein family and contains a conserved acidicN-terminal region. It has 45.5% amino acid homology to a salivaryParotid acidic protein (PRH1). PRH1 and related proline-rich (salivary)proteins act as potent inhibitors of hydroxyapatite crystal growth andbind calcium with a strength that suggests that they are important inmaintaining the concentration of ionic calcium in saliva. The N-terminalregion of salivary proline-rich proteins, which is also the region ofstrongest homology to PROL4, mediates this binding. It is expected thatPROL4 plays a role in maintaining calcium levels during bone remodeling,and that a loss of function of PROL4 facilitates bone loss. Provided isa method of treating low BMD or osteoporosis by increasing expression ofPROL4 or otherwise increasing levels of active PROL4 in a patientsuffering from low BMD or osteoporosis.

Human glutamate receptor delta-2 (GRID2) is a member of the family ofionotropic glutamate receptors that are excitatory neurotransmitterreceptors in mammalian brain. A point mutation in mouse GRID2,associated with the phenotype named “lurcher” in the heterozygous state,leads to ataxia resulting from selective, cell-autonomous apoptosis ofcerebellar Purkinje cells during postnatal development. Mice homozygousfor this mutation die shortly after birth from massive loss of mid- andhindbrain neurons during late embryogenesis. Glutamate receptors havebeen detected in osteoblasts and osteoclasts and may regulate boneresorption. Provided is a method of treating low bone mineral density orosteoporosis by modulating GRID2 function in a patient suffering fromlow BMD or osteoporosis.

PDE4D encodes cyclic AMP-dependent phosphodiesterase 4D.Phosphodiesterases are a superfamily of enzymes involved in degradationof cyclic adenosine monophosphate (cAMP) and cyclic guanosinemonophosphate (cGMP) (Manganiello V C, et al.: Arch Biochem Biophys1995, 322(1):1-13; and Beavo J A: Physiol Rev 1995, 75(4):725-748). cAMPand cGMP are important second messengers participating in the responseof various cells to hormones. In osteoblasts, cAMP produced in responseto parathyroid hormone or prostaglandins regulates osteoblasticdifferentiation (Farndale R W, et al. Biochem J 1988, 252(1):263-268;Kumegawa M, et al. Calcif Tissue Int 1984, 36(1):72-76; Ishizuya T, etal. J Clin Invest 1997, 99(12):2961-2970; and Partridge N C, et al. JCell Biochem 1994, 55(3):321-327), which leads to increases incancellous bone volume as indicated by experiments in animal models (JeeW S, et al. Bone 1987, 8(3):171-178; High W B: et al. Bone 1987,8(6):363-373; Reeve J: et al. J Bone Miner Res 1996, 11(4):440-445; andFinkelstein J S, et al. N Engl J Med 1994, 331(24): 1618-1623).Intracellular levels of cAMP are regulated by G protein-coupled adenylylcyclase (Casperson G F, Bourne H R: Annu Rev Pharmacol Toxicol 1987,27:371-384), and degradation is mediated by the phosphodiesterases. Thephosphodiesterase superfamily consists of seven families, PDE1-7,distinguished by substrate specificity, chromatographic behavior duringpurification, and affinity for biochemical activators and inhibitors. Ofthese, the PDE4 family is specific for cAMP and is selectively inhibitedby rolipram. Four PDE4 genes, 4A, 4B, 4C, and 4D, have been cloned fromrat and humans, all of which are predicted to have multiple proteinproducts due to alternate spicing of RNAs. PDE4 inhibitors have beenshown to increase bone formation in normal mice (Kinoshita T, et al.Bone 2000, 27(6):811-817) and to ameliorate loss of bone mass in animalmodels of osteopenia (Miyamoto K, et al. Biochem Pharmacol 1997,54(5):613-617; and Waki Y, et al. Jpn J Pharmacol 1999, 79(4):477-483).PDE4A and PDE4D are expressed in two common mouse osteoblastic celllines, ST2 and MC3T3-E1, that represent different stages in theosteoblast differentiation pathway (Wakabayashi S, et al. J Bone MinerRes 2002, 17(2):249-256). PDE4 inhibition with rolipram increasedBMP2-induced alkaline phosphatase activity, a marker of early osteoblastdifferentiation in ST2 cells. Furthermore, rolipram increased theexpression of alkalic phosphatase, osteopontin, collagen type I andosteocalcin in the same osteoblast precursor cells (Wakabayashi S, etal. J Bone Miner Res 2002, 17(2):249-256).

Provided herein is a method for treating osteoporosis or low bonemineral density by modulating a PDE4 function in a human suffering fromosteoporosis or low bone mineral density. Modulation of PDE4D by PDE4Dinhibitors may increase bone formation and treat an osteoporoticcondition. PDE4D inhibitors and methods of making them are described inthe following U.S. Pat. Nos. 6,218,400 (Treatment method using acGMP-Specific PDE inhibitor), 5,891,896 (Tri-substituted phenylderivatives useful as PDE IV inhibitors), 5,849,770 (Tri-substitutedphenyl derivatives useful as PDE IV inhibitors), 5,712,298(Fluoroalkoxy-substituted benzamides and their use as cyclic nucleotidephosphodiesterase inhibitors), 5,491,147 (Tri-substituted phenylderivatives and their use in pharmaceutical compositions and methods oftreatment). Also provided is a method of treating osteoporosis bydecreasing expression of PDE4D or otherwise decreasing levels of activePDE4D in a subject suffering from low BMD. Provided also is a method oftargeting pertinent information or administering preventative ortherapeutic treatments to a subject based on a subject's PDE4D genotype.

SNP rs869975 is contained within the GPX3 gene, and the TNIP3 gene maynot be ruled out due to linkage disequilibrium; therefore the GPX3/TNIP3region is considered associated with low BMD. The GPX3 gene encodes thePlasma glutathione peroxidase 3 precursor. Glutathione peroxidasecatalyzes the reduction of hydrogen peroxide, organic hydroperoxide, andlipid peroxides by reduced glutathione and functions in the protectionof cells against oxidative damage. This enzyme, found mainly in thecytosol of mammalian cells, is unusual in its content of aselenocysteine residue in its active site that is encoded by a TGA opalcodon. Selenium deficiency causes bone loss and might contribute tolower BMD. Osteoblasts produce glutathione peroxidase, possibly as adefense against hydrogen peroxide produced by osteoclasts during boneremodeling and thus may contribute to lower BMD. Provided is a methodfor treating osteoporosis or low bone mineral density by modulating aGPX function in a human suffering from osteoporosis or low bone mineraldensity.

In the TNIP1/NAF1/ABIN-1 pathway, NAF1 was identified by a yeasttwo-hybrid screen as an interacting protein to the HIV protein, Nef.Subsequently, it was found to be an A20-binding protein that is criticalfor the A20-mediated negative feedback regulation of NF-kappa Bactivation in response to tumor necrosis factor (TNF). As TNF is acritical effector of the pathogenesis of rheumatoid arthritis (RA),Gallagher et. al. (FEBS Lett. 2003 Sep. 11; 551(1-3):8-12), testedTNF-alpha-modulated gene expression in cultured primary humansynoviocytes in vitro. Genes upregulated included TNIP1 and implicateTNIP1 as a potential modulator of TNF-alpha bioactivity in RA. Thedifferentiation and functions of osteoclasts are stimulated andregulated by osteoblast/stromal cell derived factors, such as receptoractivator of NFKB ligand (RANKL). Provided is a method for treatingosteoporosis or low bone mineral density by modulating a TINIP functionin a human suffering from osteoporosis or low bone mineral density.

Low BMD and Sample Selection

The present invention is applicable to any disease in which low BMDand/or bone fracture is a factor, and is therefore particularlyconcerned with diseases such as osteoporosis. Low BMD is defined by theWorld Health Organization as 2.5 standard deviations below theage-matched mean of bone mineral density for a given population. Bonedamage may be defined as any form of structural damage such as fracturesor chips of the bone, and degradation or deterioration of the bone otherthan normal wear and tear resulting from low bone mineral density oranother cause. Such low BMD and/or bone damage is associated withosteoporosis.

Osteoporosis, or porous bone, is a disease characterized by low bonemass and structural deterioration of bone tissue, leading to bonefragility and an increased susceptibility to fractures, especially ofthe hip, spine and wrist. In general, there are two types ofosteoporosis: primary and secondary. Approximately 90% of allosteoporosis cases is idiopathic “primary osteoporosis”. Such primaryosteoporosis includes postmenopausal osteoporosis, age-associatedosteoporosis (affecting a majority of individuals over the age of 70 to80), and idiopathic osteoporosis affecting middle-aged and younger menand women. “Secondary osteoporosis” is the result of an identifiabledisease process or agent.

For some osteoporotic individuals, the loss of bone tissue issufficiently great so as to cause mechanical failure of the bonestructure. Bone fractures often occur, for example, in the hip and spineof women suffering from postmenopausal osteoporosis. Kyphosis(abnormally increased curvature of the thoracic spine) may also result.

The mechanism of bone loss in osteoporotics is believed to involve animbalance in the process of “bone remodeling”. Bone remodeling occursthroughout life, renewing the skeleton and maintaining the strength ofbone. This remodeling involves the erosion and filling of discrete siteson the surface of bones, by an organized group of cells called “basicmulticellular units” or “BMUs”. BMUs primarily consist of “osteoclasts”,“osteoblasts”, and their cellular precursors. In the remodeling cycle,bone is resorbed at the site of an “activated” BMU by an osteoclast,forming a resorption cavity. This cavity is then filled with bone by anosteoblast.

Normally, in adults, the remodeling cycle results in a small deficit inbone, due to incomplete filling of the resorption cavity. Thus, even inhealthy adults, age-related bone loss occurs. However, in osteoporotics,there is an increase in the number of BMUs that are activated. Thisincreased activation accelerates bone remodeling, resulting inabnormally high bone loss.

Preferred methods for the treatment of osteoporosis include an initialdiagnostic step to determine the presence of the disorder. Initialdiagnostic steps include determination of bone mass and rate of boneremodeling. The rate of bone remodeling can be determined by themeasurement of biochemical markers. See, for example, Hui et al., “TheContribution of Bone Loss to Postmenopausal Osteoporosis” OsteoporosisInt. 30 (1990). Diagnosis of those at risk of developing osteoporosisalso allows more effective preventive measures. Part of diagnosisincludes specialized tests called bone density tests that measure bonedensity in various sites of the body. Such methods include themeasurement of the radiodensity of skeletal radiographs, quantitativecomputerized tomography, single energy photon absorptiometry, anddual-energy photon absorptiometry. Diagnostic techniques among thoseuseful herein are described in W. A. Peck et al., Physician's ResourceManual on Osteoporosis (1987), published by the National OsteoporosisFoundation. A bone density test can detect the presence of low BMDbefore a fracture occurs, predict your chances of fracturing in thefuture, determine rate of bone loss, and monitor response to treatment.

Based in part upon selection criteria set forth above, individualshaving low BMD can be selected for genetic studies. Also, individualshaving a family history of low BMD or diagnosed with osteoporosis oftenare selected for genetic studies. Other selection criteria can include:a tissue or fluid sample derived from an individual characterized asCaucasian; sample derived from an individual of Caucasian paternal andmaternal descent, case samples derived from individuals diagnosed withosteoporosis; control samples derived from individuals with normal orhigh BMD levels and no family history of osteoporosis; and sufficientgenomic DNA for all allelotyping and genotyping reactions performedduring the study. Phenotype information may included pre- orpost-menopausal, familial predisposition, country or origin of motherand father, diagnosis with osteoporosis (date of primary diagnosis, ageof individual as of primary diagnosis, osteoporosis-related fracture),biochemical measurements of markers of bone resorption (bone-specificalkaline phosphatase, urinary C-telopeptide of type I collagen, serumosteocalcin), current medication status (thyroid medication, hormonereplacement therapy, steroid usage, bisphosphonates and cytotoxic agentsfor rheumatic diseases). Samples that meet the inclusion criteria and donot meet the exclusion criteria may be added to appropriate pools basedon gender and disease status.

Polymorphic Variants Associated with Low BMD

A genetic analysis provided herein linked low BMD with polymorphicvariant nucleic acid sequences in the human genome. As used herein, theterm “polymorphic site” refers to a region in a nucleic acid at whichtwo or more alternative nucleotide sequences are observed in asignificant number of nucleic acid samples from a population ofindividuals. A polymorphic site may be a nucleotide sequence of two ormore nucleotides, an inserted nucleotide or nucleotide sequence, adeleted nucleotide or nucleotide sequence, or a microsatellite, forexample. A polymorphic site that is two or more nucleotides in lengthmay be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or more, 20 or more,30 or more, 50 or more, 75 or more, 100 or more, 500 or more, or about1000 nucleotides in length, where all or some of the nucleotidesequences differ within the region. A polymorphic site is often onenucleotide in length, which is referred to herein as a “singlenucleotide polymorphism” or a “SNP.”

Where there are two, three, or four alternative nucleotide sequences ata polymorphic site, each nucleotide sequence is referred to as a“polymorphic variant” or “nucleic acid variant.” Where two polymorphicvariants exist, for example, the polymorphic variant represented in aminority of samples from a population is sometimes referred to as a“minor allele” and the polymorphic variant that is more prevalentlyrepresented is sometimes referred to as a “major allele.” Many organismspossess a copy of each chromosome (e.g., humans), and those individualswho possess two major alleles or two minor alleles are often referred toas being “homozygous” with respect to the polymorphism, and thoseindividuals who possess one major allele and one minor allele arenormally referred to as being “heterozygous” with respect to thepolymorphism. Individuals who are homozygous with respect to one alleleare sometimes predisposed to a different phenotype as compared toindividuals who are heterozygous or homozygous with respect to anotherallele.

In genetic analysis that associate polymorphic variants with low BMD,samples from individuals having low BMD and individuals not having lowBMD often are allelotyped and/or genotyped. The term “allelotype” asused herein refers to a process for determining the allele frequency fora polymorphic variant in pooled DNA samples from cases and controls. Bypooling DNA from each group, an allele frequency for each SNP in eachgroup is calculated. These allele frequencies are then compared to oneanother. The term “genotyped” as used herein refers to a process fordetermining a genotype of one or more individuals, where a “genotype” isa representation of one or more polymorphic variants in a population.

A genotype or polymorphic variant may be expressed in terms of a“haplotype,” which as used herein refers to two or more polymorphicvariants occurring within genomic DNA in a group of individuals within apopulation. For example, two SNPs may exist within a gene where each SNPposition includes a cytosine variation and an adenine variation. Certainindividuals in a population may carry one allele (heterozygous) or twoalleles (homozygous) having the gene with a cytosine at each SNPposition. As the two cytosines corresponding to each SNP in the genetravel together on one or both alleles in these individuals, theindividuals can be characterized as having a cytosine/cytosine haplotypewith respect to the two SNPs in the gene.

As used herein, the term “phenotype” refers to a trait which can becompared between individuals, such as presence or absence of acondition, a visually observable difference in appearance betweenindividuals, metabolic variations, physiological variations, variationsin the function of biological molecules, and the like. An example of aphenotype is occurrence of low BMD or clinically diagnosed osteoporosis.

Researchers sometimes report a polymorphic variant in a database withoutdetermining whether the variant is represented in a significant fractionof a population. Because a subset of these reported polymorphic variantsare not represented in a statistically significant portion of thepopulation, some of them are sequencing errors and/or not biologicallyrelevant. Thus, it is often not known whether a reported polymorphicvariant is statistically significant or biologically relevant until thepresence of the variant is detected in a population of individuals andthe frequency of the variant is determined. Methods for detecting apolymorphic variant in a population are described herein, specificallyin Example 2. A polymorphic variant is statistically significant andoften biologically relevant if it is represented in 5% or more of apopulation, sometimes 10% or more, 15% or more, or 20% or more of apopulation, and often 25% or more, 30% or more, 35% or more, 40% ormore, 45% or more, or 50% or more of a population.

A polymorphic variant may be detected on either or both strands of adouble-stranded nucleic acid. Also, a polymorphic variant may be locatedwithin an intron or exon of a gene or within a portion of a regulatoryregion such as a promoter, a 5′ untranslated region (UTR), a 3′ UTR, andin DNA (e.g., genomic DNA (gDNA) and complementary DNA (cDNA)), RNA(e.g., mRNA, tRNA, and rRNA), or a polypeptide. Polymorphic variationsmay or may not result in detectable differences in gene expression,polypeptide structure, or polypeptide function.

It was determined that polymorphic variations associated with anincreased risk of low BMD existed in CETP, PROL4, GRID2, PDE4D and GPX3nucleotide sequences. In the CETP locus, polymorphic variants atpositions selected from the group consisting of rs7500979, rs2217332,rs8044804, rs2270835, rs2133783, rs247609, rs952440, rs881598,rs2291955, rs2518054, rs866038, rs1436425, rs173537, rs247611, rs166017,rs173538, rs193694, rs7205692, rs8048746, rs247618, rs183130, rs6499863,rs4783961, rs3816117, rs711752, rs708272, rs1864163, rs4369653,rs1864165, rs891141, rs891143, rs7205804, rs5885, rs1532625, rs1532624,rs289712, rs7499892, rs5883, rs289714, rs158480, rs289717, rs4344729,rs289718, rs289719, rs2033254, rs4784744, rs291044, rs8053613, rs5881,rs5880, rs7198026, rs5882, rs8045701, rs289741, rs1801706, rs289742,rs289743, rs289746, rs172337, rs289747, rs1566439, rs7205459, rs289749,rs289751, rs8059220, rs8058353, rs289735, rs289737, rs291042, rs1875236,rs821466, rs821465, rs4275846, rs289707, rs821463, rs289706, rs1167741,rs2052880, rs1167742, rs1183256, rs1651665, rs1651666, rs4784751,rs1651667, rs8052091, rs1684574, rs1684575, rs1672865, rs821470,rs1549669, rs291040 and rs289754 were tested for association with lowBMD. Polymorphic variants at the following positions were associatedwith low BMD: rs166017, rs193694, rs7205804, rs1801706, rs7205459 andrs821465. At these positions in SEQ ID NO:1, a thymine at position14328, a thymine at position 14996, a guanine at position 37336, aguanine at position 50109, a thymine at position 57618, and a guanine atposition 68805 were associated with low BMD.

In the PROL4 locus, polymorphic variants at positions selected from thegroup consisting of rs523051, rs693620, rs2588349, rs2588350, rs619381,rs3759252, rs3759251, rs2418107, rs7303054, rs1838345, rs620878,rs2537817, rs1548803, rs667123, rs1838346, rs2159903, rs3944035,rs3741845, rs2110096, rs759055, rs589377, rs7960194, rs7978242,rs601051, rs4262797, rs2215714, rs1373434, rs2215715, rs612456,rs612808, rs689118, rs597468, rs592864, rs640372, rs7966559, rs654834,rs4763216, rs668521, rs669503, rs3906864, rs3906863, rs7957888,rs9300230, rs7306214, rs763839, rs2418105, rs666841, rs3851578,rs7138797, rs7295252, rs2418106, rs7299578, rs621112, rs3863320,rs1373432, rs1047699, rs1063193, rs2232959, rs2227296, rs1548804,rs2232958, rs2232957, rs2232956, rs1972571, rs3759250, rs3759249,rs1541525, rs2098248, rs2900550, rs7302130, rs4763583, rs4360778,rs1607695, rs1607694, rs2192139, rs7978300, rs7397871, rs4763217,rs2159900, rs10772370, rs7398682, rs2900551, rs2900552, rs2418214,rs2418215, rs965243, rs1117548, rs1520225, rs1520226, rs1520227,rs971919, rs2159901, rs2159902, rs2110099, rs7314847, rs7296003,rs4281556, rs4763219, rs3851579, rs3851580, rs1049119, rs2298866,rs2298865, rs2298864, rs2298863, rs3180393, rs2070837, rs7956204,rs2418216, rs3741844, rs4262798, rs2418217, rs2418218, rs7137492,rs2110100, rs1013312, rs4579993, rs1013313, rs7397106, rs2215716,rs2192140, rs4763589, rs1468697, rs2070837, rs3180393 and rs2298865 weretested for association with low BMD. Polymorphic variants at thefollowing positions were associated with an increased risk of low BMD:rs2588350, rs619381, rs620878, rs759055, rs4262797, rs612808, rs3906863,rs7957888, rs763839, rs2418105, rs666841, rs3851578, rs7299578,rs621112, rs1047699, rs1548804, rs2232956, rs1520227 and rs2215716. Atthese positions in SEQ ID NO:2, a cytosine at position 2424, a cytosineat position 3625, a guanine at position 7097, an adenine at position15688, a guanine at position 22861, a cytosine at position 24138, acytosine at position 32459, an adenine at position 35151, a guanine atposition 36930, an adenine at position 37490, a cytosine at position38432, an adenine at position 38688, a guanine at position 42665, anadenine at position 43038, a cytosine at position 49075, an adenine atposition 50773, an adenine at position 52107, a cytosine at position75246, and a guanine at position 93715 were associated with risk of lowBMD.

In the GRID2 locus, polymorphic variants at positions selected from thegroup consisting of rs1433661, rs1485009, rs7681947, rs1816432,rs1485018, rs1485017, rs7438397, rs6834311, rs1368717, rs1017391,rs2870701, rs7679839, rs1385404, rs1368716, rs4693316, rs1905707,rs1905708, rs1905709, rs3912442, rs2082553, rs6831638, rs5860329,rs2870702, rs2870703, rs1948016, rs6835836, rs1994253, rs1905710,rs1485019, rs978191, rs1385405, rs7694361, rs1905711, rs1905734,rs1485012, rs1485013, rs4692981, rs7670552, rs7670932, rs7688091,rs7440540, rs2171000, rs2870704, rs7655758, rs7661436, rs7662289,rs7667044, rs7691929, rs5860330, rs901013, rs901012, rs901011,rs1948018, rs2870705, rs1948017, rs1905733, rs1385408, rs1385409,rs1385410, rs1485026, rs1485027, rs2904483, rs1385406, rs1905732,rs2046418, rs2200377, rs1905731, rs1905730, rs975713, rs6820985,rs7670441, rs6810794, rs7676623, rs1154861, rs1032125, rs1485022,rs1485024, rs3913651, rs4693319, rs1872383, rs2200376, rs7668090,rs7692930, rs967096, rs6822249, rs6532405, rs1017897, rs7672674,rs7694568, rs2904484, rs7340830, rs1485033, rs2870706, rs1905729,rs4693320, rs6848749, rs6532406, rs6532407, rs1905728, rs6819866,rs1905727, rs7674069, rs1905724, rs1905723, rs1485020 and rs6814101 weretested for association with low BMD. Polymorphic variants at thefollowing positions were associated with an increased risk of low BMD:rs1433661, rs7679839, rs1368716, rs1905707, rs1905708, rs1994253,rs1485019, rs1905734, rs1485012, rs7670552, rs7691929, rs1948018,rs1948017, rs1485024, rs7694568, rs4693320, rs6848749, rs6532406,rs6532407 and rs6819866. At these positions in SEQ ID NO:3, a thymine atposition 206, a guanine at position 8612, an adenine at position 9285, athymine at position 11866, a guanine at position 11958, a cytosine atposition 28773, a thymine at position 29876, a cytosine at position35588, a guanine at position 37663, a thymine at position 39375, acytosine at position 43705, an adenine at position 48962, a cytosine atposition 49110, an adenine at position 65050, a cytosine at position78331, a thymine at position 85405, a guanine at position 86441, anadenine at position 86967, a cytosine at position 87121, and an adenineat position 90969 were associated with risk of low BMD.

In the PDE4D locus, polymorphic variants at positions selected from thegroup consisting of rs6886495, rs6450498, rs1472456, rs4700315,rs4700316, rs7714708, rs7710479, rs2968013, rs2968014, rs2968015,rs1391648, rs2055297, rs2055296, rs3989138, rs4700317, rs2036220,rs7727206, rs7723432, rs1546221, rs4479801, rs4395595, rs4395596,rs4699932, rs2936201, rs7356672, rs2936200, rs1909296, rs7703131,rs7445308, rs3087748, rs4321723, rs2968016, rs5868151, rs1874858,rs1874857, rs7712922, rs4631140, rs4469166, rs1078369, rs1078368,rs2968006, rs2968005, rs2936190, rs2409613, rs4415048, rs2968004,rs2968003, rs2968002, rs2936191, rs1498610, rs6874662, rs3060393,rs7729722, rs7733884, rs7714489, rs7735570, rs2936193, rs2291851,rs2291852, rs1498602, rs1995166, rs1498603, rs1498604, rs1498605,rs1948651, rs4699934, rs4700319, rs2279737, rs7720361, rs7706419,rs1006431, rs1353747, rs1498606, rs1353748, rs1553113, rs2968012,rs2968011, rs1498608, rs2936189, rs1498609, rs2968019, rs6891238,rs2968010, rs2968009, rs2936203, rs1498601, rs1498600, rs1498599,rs2936202, rs7730070, rs6450501, rs6450502, rs6889456, rs6894618,rs7706044, rs7707541, rs7712076, rs6892860, rs6867053, rs7737269,rs6864156, rs950447, rs2936196, rs7719347, rs1391649, rs1391650,rs1391651, rs1353749, rs10682149, rs5868153, rs1363882, rs2409626,rs2968018, rs954740, rs986067, rs6869400, and rs5010782 were tested forassociation with low BMD. Polymorphic variants at the followingpositions were associated with an increased risk of low BMD: rs7714708,rs1498602, rs4699934, rs1006431, rs1353747, rs1498608, rs1498609,rs2968010, rs2936202 and rs1391649. At these positions in SEQ ID NO:4,an adenine at position 1599, a cytosine at position 39626, a thymine atposition 40356, a thymine at position 43555, a thymine at position44066, a thymine at position 49652, a cytosine at position 51103, anadenine at position 57173, a guanine at position 63980, and an adenineat position 82591 were associated with risk of low BMD.

In the GPX3/TNIP1 locus, polymorphic variants at positions selected fromthe group consisting of rs1478398, rs1478397, rs1160114, rs1160113,rs1382323, rs1160112, rs7709870, rs7710643, rs7730467, rs6579829,rs6579830, rs6579831, rs6896232, rs1351131, rs1038074, rs1478396,rs6880512, rs4958858, rs4958431, rs4958432, rs6898463, rs4958859,rs4130064, rs4130065, rs4133119, rs4958860, rs4958861, rs4437356,rs4958868, rs1478400, rs6889375, rs1600159, rs6875892, rs4608909,rs2345000, rs4516840, rs2054440, rs707141, rs707142, rs841236, rs707143,rs70144, rs6869405, rs707145, rs707146, rs707148, rs707150, rs5872184,rs3763015, rs2042235, rs3763013, rs2042236, rs1946234, rs1946235,rs1946236, rs8177402, rs8177403, rs8177404, rs8177405, rs8177406,rs8177407, rs8177408, rs8177409, rs6888961, rs8177410, rs8177411,rs8177412, rs8177413, rs870407, rs870406, rs6873202, rs8177414,rs8177415, rs3805435, rs8177416, rs3792799, rs3792798, rs3828599,rs8177417, rs3792797, rs8177418, rs8177419, rs8177420, rs8177421,rs4958872, rs3792796, rs8177422, rs8177423, rs4958434, rs8177424,rs8177425, rs8177426, rs8177427, rs8177429, rs6889737, rs3792795,rs8177430, rs8177431, rs4958873, rs8177432, rs8177433, rs8177434,rs8177435, rs3763011, rs8177436, rs8177437, rs4958874, rs8177439,rs8177440, rs8177441, rs8177442, rs8177443, rs869975, rs869976,rs8177444, rs8177445, rs7721469, rs8177446, rs7704191, rs8177447,rs11548, rs2230303, rs7722386, rs8177448, rs8177449, rs2070593,rs8177450, rs8177451, rs8177452, rs8177453, rs8177454, rs3763010,rs8177455, rs8177456, rs736775, rs2277940, rs8177458, rs8177834, rs3924,rs2233312, rs2233311, rs2233310, rs2233309, rs4958875, rs2233308,rs2233307, rs2233306, rs2233305, rs2233304, rs2233303, rs2233302,rs2287719, rs2287720, rs7727034, rs7727250, rs7709800, rs3840312,rs2287721, rs6875293, rs3805434, rs2080982, rs2080983, rs2287722,rs2233301, rs2233300, rs4958876, rs2233299, rs2233298, rs2287723,rs2161359, rs7734456, rs4292439, rs4958878, rs6862024, rs3834819,rs2233297, rs2233296, rs2233295, rs2233294, rs7713028, rs7713223,rs7713567, rs888989, rs2233293, rs3749657, rs2233292, rs2112635,rs871269, rs3792794, rs6579837, rs3805433, rs5872186, rs2233291,rs2233290, rs2233289, rs4958435, rs4958880, rs1422673, rs2042234,rs3805432, rs3805431, rs2233288, rs2233287, rs3815720, rs3792792,rs3792791, rs2303018, rs3792790, rs4958436, rs2233286, rs2233285,rs7732451, rs2233284, rs1422674, rs3792789, rs4562032, rs6865077,rs1559126, rs3792788, rs1559127, rs3792786, rs6880110, rs6861227,rs3805430, rs1862364, rs4958881, rs3792785, rs6869605, rs6870205,rs4246047, rs4958882, rs3792784, rs3792783 and rs5872188 were tested forassociation with low BMD. Polymorphic variants at the followingpositions were associated with an increased risk of low BMD: rs1478398,rs1160114, rs1160113, rs1160112, rs4958858, rs4958431, rs6898463,rs4958859, rs4958860, rs4608909, rs707144, rs2042235, rs3763013,rs2042236, rs8177404, rs8177426, rs8177427, rs8177429, rs3792795,rs4958873, rs8177437, rs869975, rs8177447, rs11548, rs2277940,rs8177834, rs2233311, rs2233302, rs7727034, rs7727250, rs3805434,rs7734456, rs7713028, rs7713223, rs888989, rs3792794, rs4958880,rs1422673, rs3805432 and rs4958436. At these positions in SEQ ID NO:5,an adenine at position 231, a cytosine at position 582, a guanine atposition 589, an adenine at position 1066, a thymine at position 5621, aguanine at position 5735, a cytosine at position 6658, a cytosine atposition 7901, a thymine at position 15803, a cytosine at position25599, a thymine at position 31203, a thymine at position 41624, aguanine at position 41671, an adenine at position 41825, a cytosine atposition 43294, an adenine at position 46650, an adenine at position46721, a guanine at position 46808, a guanine at position 47512, anadenine at position 47806, a guanine at position 49097, a guanine atposition 50082, a thymine at position 51166, a cytosine at position51493, a thymine at position 53187, an adenine at position 53699, athymine at position 53929, a cytosine at position 58808, a cytosine atposition 59187, a cytosine at position 59361, a cytosine at position64049, a guanine at position 70882, an adenine at position 74131, acytosine at position 74406, a cytosine at position 74740, a guanine atposition 78432, a cytosine at position 82187, a cytosine at position82698, an adenine at position 83214 and a thymine at position 86539 wereassociated with risk of low BMD.

Based in part upon analyses summarized in FIGS. 1-5, regions withsignificant association have been identified in loci associated with lowBMD. Any polymorphic variants associated with low BMD in a region ofsignificant association can be utilized for embodiments describedherein. The following table reports such regions, where “begin” and“end” designate the boundaries of the region according to chromosomepositions within the genomic sequence provided in SEQ ID Nos:1-5. Thelocus, the chromosome on which the locus resides and an incidentpolymorphism in the locus also are noted. TABLE 1 COMBINED ASSOCIATIONRANGES Incident SNP Locus Chromosome Begin End Size rs1801706 CETP 16q2114328 68805 54477 rs1047699 PROL4 12p13 2424 93715 91291 rs1948017 GRID24q22 206 90969 90763 rs1498608 PDE4D 5q12 1599 82591 80992 rs869975GPX3/TNIP1 5q23 231 86539 86308

Additional Polymorphic Variants Associated with Low BMD

Also provided is a method for identifying polymorphic variants proximalto an incident, founder polymorphic variant associated with low BMD.Thus, featured herein are methods for identifying a polymorphicvariation associated with low BMD that is proximal to an incidentpolymorphic variation associated with low BMD, which comprisesidentifying a polymorphic variant proximal to the incident polymorphicvariant associated with low BMD, where the incident polymorphic variantis in a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence. Thenucleotide sequence often comprises a polynucleotide sequence selectedfrom the group consisting of (a) a polynucleotide sequence of SEQ IDNO's:1-5; (b) a polynucleotide sequence that encodes a polypeptidehaving an amino acid sequence encoded by a polynucleotide sequence ofSEQ ID NO's:1-5; and (c) a polynucleotide sequence that encodes apolypeptide having an amino acid sequence that is 90% or more identicalto an amino acid sequence encoded by a nucleotide sequence of SEQ IDNO's:1-5 or a polynucleotide sequence 90% or more identical to thepolynucleotide sequence of SEQ ID NO's:1-5. The presence or absence ofan association of the proximal polymorphic variant with low BMD then isdetermined using a known association method, such as a method describedin the Examples hereafter. In an embodiment, the incident polymorphicvariant is a polymorphic variant associated with low BMD describedherein. In another embodiment, the proximal polymorphic variantidentified sometimes is a publicly disclosed polymorphic variant, whichfor example, sometimes is published in a publicly available database. Inother embodiments, the polymorphic variant identified is not publiclydisclosed and is discovered using a known method, including, but notlimited to, sequencing a region surrounding the incident polymorphicvariant in a group of nucleic samples. Thus, multiple polymorphicvariants proximal to an incident polymorphic variant are associated withlow BMD using this method.

The proximal polymorphic variant often is identified in a regionsurrounding the incident polymorphic variant. In certain embodiments,this surrounding region is about 50 kb flanking the first polymorphicvariant (e.g. about 50 kb 5′ of the first polymorphic variant and about50 kb 3′ of the first polymorphic variant), and the region sometimes iscomposed of shorter flanking sequences, such as flanking sequences ofabout 40 kb, about 30 kb, about 25 kb, about 20 kb, about 15 kb, about10 kb, about 7 kb, about 5 kb, or about 2 kb 5′ and 3′ of the incidentpolymorphic variant. In other embodiments, the region is composed oflonger flanking sequences, such as flanking sequences of about 55 kb,about 60 kb, about 65 kb, about 70 kb, about 75 kb, about 80 kb, about85 kb, about 90 kb, about 95 kb, or about 100 kb 5′ and 3′ of theincident polymorphic variant.

In certain embodiments, polymorphic variants associated with low BMD areidentified iteratively. For example, a first proximal polymorphicvariant is associated with low BMD using the methods described above andthen another polymorphic variant proximal to the first proximalpolymorphic variant is identified (e.g., publicly disclosed ordiscovered) and the presence or absence of an association of one or moreother polymorphic variants proximal to the first proximal polymorphicvariant with low BMD is determined.

The methods described herein are useful for identifying or discoveringadditional polymorphic variants that may be used to further characterizea gene, region or loci associated with a condition, a disease (e.g.,osteoporosis), or a disorder. For example, allelotyping or genotypingdata from the additional polymorphic variants may be used to identify afunctional mutation or a region of linkage disequilibrium. In certainembodiments, polymorphic variants identified or discovered within aregion comprising the first polymorphic variant associated with low BMDare genotyped using the genetic methods and sample selection techniquesdescribed herein, and it can be determined whether those polymorphicvariants are in linkage disequilibrium with the first polymorphicvariant. The size of the region in linkage disequilibrium with the firstpolymorphic variant also can be assessed using these genotyping methods.Thus, provided herein are methods for determining whether a polymorphicvariant is in linkage disequilibrium with a first polymorphic variantassociated with low BMD, and such information can be used inprognosis/diagnosis methods described herein.

Isolated Nucleic Acids

Featured herein are isolated CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleic acid variants depicted in SEQ ID NO's:1-10, and substantiallyidentical nucleic acids thereof. A nucleic acid variant may berepresented on one or both strands in a double-stranded nucleic acid oron one chromosomal complement (heterozygous) or both chromosomalcomplements (homozygous)).

As used herein, the term “nucleic acid” includes DNA molecules (e.g., acomplementary DNA (cDNA) and genomic DNA (gDNA)) and RNA molecules(e.g., mRNA, rRNA, siRNA and tRNA) and analogs of DNA or RNA, forexample, by use of nucleotide analogs. The nucleic acid molecule can besingle-stranded and it is often double-stranded. The term “isolated orpurified nucleic acid” refers to nucleic acids that are separated fromother nucleic acids present in the natural source of the nucleic acid.For example, with regard to genomic DNA, the term “isolated” includesnucleic acids which are separated from the chromosome with which thegenomic DNA is naturally associated. An “isolated” nucleic acid is oftenfree of sequences which naturally flank the nucleic acid (i.e.,sequences located at the 5′ and/or 3′ ends of the nucleic acid) in thegenomic DNA of the organism from which the nucleic acid is derived. Forexample, in various embodiments, the isolated nucleic acid molecule cancontain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kbof 5′ and/or 3′ nucleotide sequences which flank the nucleic acidmolecule in genomic DNA of the cell from which the nucleic acid isderived. Moreover, an “isolated” nucleic acid molecule, such as a cDNAmolecule, can be substantially free of other cellular material, orculture medium when produced by recombinant techniques, or substantiallyfree of chemical precursors or other chemicals when chemicallysynthesized. As used herein, the term “gene” refers to a nucleotidesequence that encodes a polypeptide.

The nucleic acid often comprises a part of or all of a nucleotidesequence in SEQ ID NO's:1-5, or a substantially identical sequencethereof. Such a nucleotide sequence sometimes is a 5′ and/or 3′ sequenceflanking a polymorphic variant described above that is 5-1000nucleotides in length, or in some embodiments 5-500, 5-100, 5-75, 5-50,5-45, 5-40, 5-35, 5-30, 5-25 or 5-20 nucleotides in length. Otherembodiments are directed to methods of identifying a polymorphicvariation at one or more positions in a nucleic acid (e.g., genotypingat one or more positions in the nucleic acid), such as at a positioncorresponding to rs1801706 in the CETP gene, rs1047699 in the PROL4gene, rs1948017 in the GRID2 gene, rs1498608 in the PDE4D gene, orrs869975 in the GPX3 gene.

Also included herein are nucleic acid fragments. These fragments oftenare a nucleotide sequence identical to a nucleotide sequence of SEQ IDNO's:1-10, a nucleotide sequence substantially identical to a nucleotidesequence of SEQ ID NO's:1-10, or a nucleotide sequence that iscomplementary to the foregoing. The nucleic acid fragment may beidentical, substantially identical or homologous to a nucleotidesequence in an exon or an intron in a nucleotide sequence of SEQ IDNO's:1-5, and may encode a domain or part of a domain of a polypeptide.Sometimes, the fragment will comprises one or more of the polymorphicvariations described herein as being associated with low BMD. Thenucleic acid fragment is often 50, 100, or 200 or fewer base pairs inlength, and is sometimes about 300, 400, 500, 600, 700, 800, 900, 1000,1100, 1200, 1300, 1400, 1500, 2000, 3000, 4000, 5000, 10000, 15000, or20000 base pairs in length. A nucleic acid fragment that iscomplementary to a nucleotide sequence identical or substantiallyidentical to a nucleotide sequence in SEQ ID NO's:1-5 and hybridizes tosuch a nucleotide sequence under stringent conditions is often referredto as a “probe.” Nucleic acid fragments often include one or morepolymorphic sites, or sometimes have an end that is adjacent to apolymorphic site as described hereafter. CETP nucleic acid fragmentssometimes encode the mature protein from positions 182 to 1609 of themRNA sequence (SEQ ID NO: 6), for example.

An example of a nucleic acid fragment is an oligonucleotide. As usedherein, the term “oligonucleotide” refers to a nucleic acid comprisingabout 8 to about 50 covalently linked nucleotides, often comprising fromabout 8 to about 35 nucleotides, and more often from about 10 to about25 nucleotides. The backbone and nucleotides within an oligonucleotidemay be the same as those of naturally occurring nucleic acids, oranalogs or derivatives of naturally occurring nucleic acids, providedthat oligonucleotides having such analogs or derivatives retain theability to hybridize specifically to a nucleic acid comprising atargeted polymorphism. Oligonucleotides described herein may be used ashybridization probes or as components of prognostic or diagnosticassays, for example, as described herein.

Oligonucleotides are typically synthesized using standard methods andequipment, such as the ABI™3900 High Throughput DNA Synthesizer and theEXPEDITE™ 8909 Nucleic Acid Synthesizer, both of which are availablefrom Applied Biosystems (Foster City, Calif.). Analogs and derivativesare exemplified in U.S. Pat. Nos. 4,469,863; 5,536,821; 5,541,306;5,637,683; 5,637,684; 5,700,922; 5,717,083; 5,719,262; 5,739,308;5,773,601; 5,886,165; 5,929,226; 5,977,296; 6,140,482; WO 00/56746; WO01/14398, and related publications. Methods for synthesizingoligonucleotides comprising such analogs or derivatives are disclosed,for example, in the patent publications cited above and in U.S. Pat.Nos. 5,614,622; 5,739,314; 5,955,599; 5,962,674; 6,117,992; in WO00/75372; and in related publications.

Oligonucleotides may also be linked to a second moiety. The secondmoiety may be an additional nucleotide sequence such as a tail sequence(e.g., a polyadenosine tail), an adapter sequence (e.g., phage M13universal tail sequence), and others. Alternatively, the second moietymay be a non-nucleotide moiety such as a moiety which facilitateslinkage to a solid support or a label to facilitate detection of theoligonucleotide. Such labels include, without limitation, a radioactivelabel, a fluorescent label, a chemiluminescent label, a paramagneticlabel, and the like. The second moiety may be attached to any positionof the oligonucleotide, provided the oligonucleotide can hybridize tothe nucleic acid comprising the polymorphism.

Uses of Nucleic Acid Sequence

Nucleic acid coding sequences (e.g., SEQ ID NO: 7-12) may be used fordiagnostic purposes for detection and control of polypeptide expression.Also, included herein are oligonucleotide sequences such as antisenseRNA, small-interfering RNA (siRNA) and DNA molecules and ribozymes thatfunction to inhibit translation of a polypeptide. Antisense techniquesand RNA interference techniques are known in the art and are describedherein.

Ribozymes are enzymatic RNA molecules capable of catalyzing the specificcleavage of RNA. The mechanism of ribozyme action involves sequencespecific hybridization of the ribozyme molecule to complementary targetRNA, followed by endonucleolytic cleavage. For example, hammerhead motifribozyme molecules may be engineered that specifically and efficientlycatalyze endonucleolytic cleavage of RNA sequences corresponding to orcomplementary to CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotidesequences. Specific ribozyme cleavage sites within any potential RNAtarget are initially identified by scanning the target molecule forribozyme cleavage sites which include the following sequences, GUA, GUUand GUC. Once identified, short RNA sequences of between fifteen (15)and twenty (20) ribonucleotides corresponding to the region of thetarget gene containing the cleavage site may be evaluated for predictedstructural features such as secondary structure that may render theoligonucleotide sequence unsuitable. The suitability of candidatetargets may also be evaluated by testing their accessibility tohybridization with complementary oligonucleotides, using ribonucleaseprotection assays.

Antisense RNA and DNA molecules, siRNA and ribozymes may be prepared byany method known in the art for the synthesis of RNA molecules. Theseinclude techniques for chemically synthesizing oligodeoxyribonucleotideswell known in the art such as solid phase phosphoramidite chemicalsynthesis. Alternatively, RNA molecules may be generated by in vitro andin vivo transcription of DNA sequences encoding the antisense RNAmolecule. Such DNA sequences may be incorporated into a wide variety ofvectors which incorporate suitable RNA polymerase promoters such as theT7 or SP6 polymerase promoters. Alternatively, antisense cDNA constructsthat synthesize antisense RNA constitutively or inducibly, depending onthe promoter used, can be introduced stably into cell lines.

DNA encoding a polypeptide also may have a number of uses for thediagnosis of diseases, including low BMD, resulting from aberrantexpression of a target gene described herein. For example, the nucleicacid sequence may be used in hybridization assays of biopsies orautopsies to diagnose abnormalities of expression or function (e.g.,Southern or Northern blot analysis, in situ hybridization assays).

In addition, the expression of a polypeptide during embryonicdevelopment may also be determined using nucleic acid encoding thepolypeptide. As addressed, infra, production of functionally impairedpolypeptide is the cause of various disease states, such asosteoporosis. In situ hybridizations using polypeptide as a probe may beemployed to predict problems related to low BMD. Further, as indicated,infra, administration of human active polypeptide, recombinantlyproduced as described herein, may be used to treat disease statesrelated to functionally impaired polypeptide. Alternatively, genetherapy approaches may be employed to remedy deficiencies of functionalpolypeptide or to replace or compete with dysfunctional polypeptide.

Expression Vectors, Host Cells, and Genetically Engineered Cells

Provided herein are nucleic acid vectors, often expression vectors,which contain a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotidesequence or a substantially identical sequence thereof. As used herein,the term “vector” refers to a nucleic acid molecule capable oftransporting another nucleic acid to which it has been linked and caninclude a plasmid, cosmid, or viral vector. The vector can be capable ofautonomous replication or it can integrate into a host DNA. Viralvectors may include replication defective retroviruses, adenoviruses andadeno-associated viruses for example.

A vector can include a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleotide sequence in a form suitable for expression of an encodedtarget polypeptide or target nucleic acid in a host cell. A “targetpolypeptide” is a polypeptide encoded by a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence or a substantially identical nucleotidesequence thereof. The recombinant expression vector typically includesone or more regulatory sequences operatively linked to the nucleic acidsequence to be expressed. The term “regulatory sequence” includespromoters, enhancers and other expression control elements (e.g.,polyadenylation signals). Regulatory sequences include those that directconstitutive expression of a nucleotide sequence, as well astissue-specific regulatory and/or inducible sequences. The design of theexpression vector can depend on such factors as the choice of the hostcell to be transformed, the level of expression of polypeptide desired,and the like. Expression vectors can be introduced into host cells toproduce target polypeptides, including fusion polypeptides.

Recombinant expression vectors can be designed for expression of targetpolypeptides in prokaryotic or eukaryotic cells. For example, targetpolypeptides can be expressed in E. coli, insect cells (e.g., usingbaculovirus expression vectors), yeast cells, or mammalian cells.Suitable host cells are discussed further in Goeddel, Gene ExpressionTechnology: Methods in Enzymology 185, Academic Press, San Diego, Calif.(1990). Alternatively, the recombinant expression vector can betranscribed and translated in vitro, for example using T7 promoterregulatory sequences and T7 polymerase.

Expression of polypeptides in prokaryotes is most often carried out inE. coli with vectors containing constitutive or inducible promotersdirecting the expression of either fusion or non-fusion polypeptides.Fusion vectors add a number of amino acids to a polypeptide encodedtherein, usually to the amino terminus of the recombinant polypeptide.Such fusion vectors typically serve three purposes: 1) to increaseexpression of recombinant polypeptide; 2) to increase the solubility ofthe recombinant polypeptide; and 3) to aid in the purification of therecombinant polypeptide by acting as a ligand in affinity purification.Often, a proteolytic cleavage site is introduced at the junction of thefusion moiety and the recombinant polypeptide to enable separation ofthe recombinant polypeptide from the fusion moiety subsequent topurification of the fusion polypeptide. Such enzymes, and their cognaterecognition sequences, include Factor Xa, thrombin and enterokinase.Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc;Smith & Johnson, Gene 67: 31-40 (1988)), pMAL (New England Biolabs,Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) which fuseglutathione S-transferase (GST), maltose E binding polypeptide, orpolypeptide A, respectively, to the target recombinant polypeptide.

Purified fusion polypeptides can be used in screening assays and togenerate antibodies specific for target polypeptides. In a therapeuticembodiment, fusion polypeptide expressed in a retroviral expressionvector is used to infect bone marrow cells that are subsequentlytransplanted into irradiated recipients. The pathology of the subjectrecipient is then examined after sufficient time has passed (e.g., six(6) weeks).

Expressing the polypeptide in host bacteria with an impaired capacity toproteolytically cleave the recombinant polypeptide is often used tomaximize recombinant polypeptide expression (Gottesman, S., GeneExpression Technology: Methods in Enzymology, Academic Press, San Diego,Calif. 185: 119-128 (1990)). Another strategy is to alter the nucleotidesequence of the nucleic acid to be inserted into an expression vector sothat the individual codons for each amino acid are those preferentiallyutilized in E. coli (Wada et al., Nucleic Acids Res. 20: 2111-2118(1992)). Such alteration of nucleotide sequences can be carried out bystandard DNA synthesis techniques.

When used in mammalian cells, the expression vector's control functionsare often provided by viral regulatory elements. For example, commonlyused promoters are derived from polyoma, Adenovirus 2, cytomegalovirusand Simian Virus 40. Recombinant mammalian expression vectors are oftencapable of directing expression of the nucleic acid in a particular celltype (e.g., tissue-specific regulatory elements are used to express thenucleic acid). Non-limiting examples of suitable tissue-specificpromoters include an albumin promoter (liver-specific; Pinkert et al.,Genes Dev. 1: 268-277 (1987)), lymphoid-specific promoters (Calame &Eaton, Adv. Immunol. 43: 235-275 (1988)), promoters of T cell receptors(Winoto & Baltimore, EMBO J. 8: 729-733 (1989)) promoters ofimmunoglobulins (Banerji et al., Cell 33: 729-740 (1983); Queen &Baltimore, Cell 33: 741-948 (1983)), neuron-specific promoters (e.g.,the neurofilament promoter; Byrne & Ruddle, Proc. Natl. Acad. Sci. USA86: 5473-5477 (1989)), pancreas-specific promoters (Edlund et al.,Science 230: 912-916 (1985)), and mammary gland-specific promoters(e.g., milk whey promoter; U.S. Pat. No. 4,873,316 and EuropeanApplication Publication No. 264,166). Developmentally-regulatedpromoters are sometimes utilized, for example, the murine hox promoters(Kessel & Gruss, Science 249: 374-379 (1990)) and the α-fetopolypeptidepromoter (Campes & Tilghman, Genes Dev. 3: 537-546 (1989)).

A CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid may also becloned into an expression vector in an antisense orientation. Regulatorysequences (e.g., viral promoters and/or enhancers) operatively linked toa CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid cloned in theantisense orientation can be chosen for directing constitutive, tissuespecific or cell type specific expression of antisense RNA in a varietyof cell types. Antisense expression vectors can be in the form of arecombinant plasmid, phagemid or attenuated virus. For a discussion ofthe regulation of gene expression using antisense genes see, e.g.,Weintraub et al., Antisense RNA as a molecular tool for geneticanalysis, Reviews—Trends in Genetics, Vol. 1(1) (1986).

Also provided herein are host cells that include a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleotide sequence within a recombinant expressionvector or a fragment of such a nucleotide sequence which facilitatehomologous recombination into a specific site of the host cell genome.The terms “host cell” and “recombinant host cell” are usedinterchangeably herein. Such terms refer not only to the particularsubject cell but rather also to the progeny or potential progeny of sucha cell. Because certain modifications may occur in succeedinggenerations due to either mutation or environmental influences, suchprogeny may not, in fact, be identical to the parent cell, but are stillincluded within the scope of the term as used herein. A host cell can beany prokaryotic or eukaryotic cell. For example, a target polypeptidecan be expressed in bacterial cells such as E. coli, insect cells, yeastor mammalian cells (such as Chinese hamster ovary cells (CHO) or COScells). Other suitable host cells are known to those skilled in the art.

Vectors can be introduced into host cells via conventionaltransformation or transfection techniques. As used herein, the terms“transformation” and “transfection” are intended to refer to a varietyof art-recognized techniques for introducing foreign nucleic acid (e.g.,DNA) into a host cell, including calcium phosphate or calcium chlorideco-precipitation, transduction/infection, DEAE-dextran-mediatedtransfection, lipofection, or electroporation.

A host cell provided herein can be used to produce (i.e., express) atarget polypeptide or a substantially identical polypeptide thereof.Accordingly, further provided are methods for producing a targetpolypeptide using host cells described herein. In one embodiment, themethod includes culturing host cells into which a recombinant expressionvector encoding a target polypeptide has been introduced in a suitablemedium such that a target polypeptide is produced. In anotherembodiment, the method further includes isolating a target polypeptidefrom the medium or the host cell.

Also provided are cells or purified preparations of cells which includea CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 transgene, or which otherwisemisexpress target polypeptide. Cell preparations can consist of human ornon-human cells, e.g., rodent cells, e.g., mouse or rat cells, rabbitcells, or pig cells. In preferred embodiments, the cell or cells includea CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 transgene (e.g., aheterologous form of a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 gene,such as a human gene expressed in non-human cells). The transgene can bemisexpressed, e.g., overexpressed or underexpressed. In other preferredembodiments, the cell or cells include a gene which misexpress anendogenous target polypeptide (e.g., expression of a gene is disrupted,also known as a knockout). Such cells can serve as a model for studyingdisorders which are related to mutated or mis-expressed alleles or foruse in drug screening. Also provided are human cells (e.g., ahematopoietic stem cells) transformed with a CETP, PROL4, GRID2, PDE4Dor GPX3/TNIP1 nucleic acid.

Also provided are cells or a purified preparation thereof (e.g., humancells) in which an endogenous CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleic acid is under the control of a regulatory sequence that does notnormally control the expression of the endogenous gene corresponding toa CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence. Theexpression characteristics of an endogenous gene within a cell (e.g., acell line or microorganism) can be modified by inserting a heterologousDNA regulatory element into the genome of the cell such that theinserted regulatory element is operably linked to the correspondingendogenous gene. For example, an endogenous corresponding gene (e.g., agene which is “transcriptionally silent,” not normally expressed, orexpressed only at very low levels) may be activated by inserting aregulatory element which is capable of promoting the expression of anormally expressed gene product in that cell. Techniques such astargeted homologous recombinations, can be used to insert theheterologous DNA as described in, e.g., Chappel, U.S. Pat. No.5,272,071; WO 91/06667, published on May 16, 1991.

Transyenic Animals

Non-human transgenic animals that express a heterologous targetpolypeptide (e.g., expressed from a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleic acid or substantially identical sequence thereof) canbe generated. Such animals are useful for studying the function and/oractivity of a target polypeptide and for identifying and/or evaluatingmodulators of the activity of CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleic acids and encoded polypeptides. As used herein, a “transgenicanimal” is a non-human animal such as a mammal (e.g., a non-humanprimate such as chimpanzee, baboon, or macaque; an ungulate such as anequine, bovine, or caprine; or a rodent such as a rat, a mouse, or anIsraeli sand rat), a bird (e.g., a chicken or a turkey), an amphibian(e.g., a frog, salamander, or newt), or an insect (e.g., Drosophilamelanogaster), in which one or more of the cells of the animal includesa transgene. A transgene is exogenous DNA or a rearrangement (e.g., adeletion of endogenous chromosomal DNA) that is often integrated into oroccurs in the genome of cells in a transgenic animal. A transgene candirect expression of an encoded gene product in one or more cell typesor tissues of the transgenic animal, and other transgenes can reduceexpression (e.g., a knockout). Thus, a transgenic animal can be one inwhich an endogenous nucleic acid homologous to a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleic acid has been altered by homologousrecombination between the endogenous gene and an exogenous DNA moleculeintroduced into a cell of the animal (e.g., an embryonic cell of theanimal) prior to development of the animal.

Intronic sequences and polyadenylation signals can also be included inthe transgene to increase expression efficiency of the transgene. One ormore tissue-specific regulatory sequences can be operably linked to aCETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence to directexpression of an encoded polypeptide to particular cells. A transgenicfounder animal can be identified based upon the presence of a CETP,PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence in its genomeand/or expression of encoded mRNA in tissues or cells of the animals. Atransgenic founder animal can then be used to breed additional animalscarrying the transgene. Moreover, transgenic animals carrying a CETP,PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence can further bebred to other transgenic animals carrying other transgenes.

Target polypeptides can be expressed in transgenic animals or plants byintroducing, for example, a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleic acid into the genome of an animal that encodes the targetpolypeptide. In preferred embodiments the nucleic acid is placed underthe control of a tissue specific promoter, e.g., a milk or egg specificpromoter, and recovered from the milk or eggs produced by the animal.Also included is a population of cells from a transgenic animal.

Target Polypeptides

Also featured herein are isolated target polypeptides, which are encodedby a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence (e.g.,SEQ ID NO's:1-10) or a substantially identical nucleotide sequencethereof, such as the polypeptides having amino acid sequences in SEQ IDNO's:11-15. The term “polypeptide” as used herein includes proteins andpeptides. An “isolated” or “purified” polypeptide or protein issubstantially free of cellular material or other contaminating proteinsfrom the cell or tissue source from which the protein is derived, orsubstantially free from chemical precursors or other chemicals whenchemically synthesized. In one embodiment, the language “substantiallyfree” means preparation of a target polypeptide having less than about30%, 20%, 10% and more preferably 5% (by dry weight), of non-targetpolypeptide (also referred to herein as a “contaminating protein”), orof chemical precursors or non-target chemicals. When the targetpolypeptide or a biologically active portion thereof is recombinantlyproduced, it is also preferably substantially free of culture medium,specifically, where culture medium represents less than about 20%,sometimes less than about 10%, and often less than about 5% of thevolume of the polypeptide preparation. Isolated or purified targetpolypeptide preparations are sometimes 0.01 milligrams or more or 0.1milligrams or more, and often 1.0 milligrams or more and 10 milligramsor more in dry weight.

Further included herein are target polypeptide fragments. Thepolypeptide fragment may be a domain or part of a domain of a targetpolypeptide. In addition, the polypeptide fragment may be a full-lengthpolypeptide or a mature polypeptide (i.e., the polypeptide minus thesignal peptide). For example, a fragment sometimes is a CTEP matureprotein that corresponds to amino acid positions 18493 of SEQ ID NO:11.The polypeptide fragment may have increased, decreased or unexpectedbiological activity. The polypeptide fragment is often 50 or fewer, 100or fewer, or 200 or fewer amino acids in length, and is sometimes 300,400, 500, 600, 700, or 900 or fewer amino acids in length.

Substantially identical target polypeptides may depart from the aminoacid sequences of target polypeptides in different manners. For example,conservative amino acid modifications may be introduced at one or morepositions in the amino acid sequences of target polypeptides. A“conservative amino acid substitution” is one in which the amino acid isreplaced by another amino acid having a similar structure and/orchemical function. Families of amino acid residues having similarstructures and functions are well known. These families include aminoacids with basic side chains (e.g., lysine, arginine, histidine), acidicside chains (e.g., aspartic acid, glutamic acid), uncharged polar sidechains (e.g., glycine, asparagine, glutamine, serine, threonine,tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine,leucine, isoleucine, proline, phenylalanine, methionine, tryptophan),beta-branched side chains (e.g., threonine, valine, isoleucine) andaromatic side chains (e.g., tyrosine, phenylalanine, tryptophan,histidine). Also, essential and non-essential amino acids may bereplaced. A “non-essential” amino acid is one that can be alteredwithout abolishing or substantially altering the biological function ofa target polypeptide, whereas altering an “essential” amino acidabolishes or substantially alters the biological function of a targetpolypeptide. Amino acids that are conserved among target polypeptidesare typically essential amino acids.

Also, target polypeptides may exist as chimeric or fusion polypeptides.As used herein, a target “chimeric polypeptide” or target “fusionpolypeptide” includes a target polypeptide linked to a non-targetpolypeptide. A “non-target polypeptide” refers to a polypeptide havingan amino acid sequence corresponding to a polypeptide which is notsubstantially identical to the target polypeptide, which includes, forexample, a polypeptide that is different from the target polypeptide andderived from the same or a different organism. The target polypeptide inthe fusion polypeptide can correspond to an entire or nearly entiretarget polypeptide or a fragment thereof. The non-target polypeptide canbe fused to the N-terminus or C-terminus of the target polypeptide.

Fusion polypeptides can include a moiety having high affinity for aligand. For example, the fusion polypeptide can be a GST-target fusionpolypeptide in which the target sequences are fused to the C-terminus ofthe GST sequences, or a polyhistidine-target fusion polypeptide in whichthe target polypeptide is fused at the N- or C-terminus to a string ofhistidine residues. Such fusion polypeptides can facilitate purificationof recombinant target polypeptide. Expression vectors are commerciallyavailable that already encode a fusion moiety (e.g., a GST polypeptide),and a nucleotide sequence in SEQ ID NO's:1-10, or a substantiallyidentical nucleotide sequence thereof, can be cloned into an expressionvector such that the fusion moiety is linked in-frame to the targetpolypeptide. Further, the fusion polypeptide can be a target polypeptidecontaining a heterologous signal sequence at its N-terminus. In certainhost cells (e.g., mammalian host cells), expression, secretion, cellularinternalization, and cellular localization of a target polypeptide canbe increased through use of a heterologous signal sequence. Fusionpolypeptides can also include all or a part of a serum polypeptide(e.g., an IgG constant region or human serum albumin).

Target polypeptides can be incorporated into pharmaceutical compositionsand administered to a subject in vivo. Administration of these targetpolypeptides can be used to affect the bioavailability of a substrate ofthe target polypeptide and may effectively increase target polypeptidebiological activity in a cell. Target fusion polypeptides may be usefultherapeutically for the treatment of disorders caused by, for example,(i) aberrant modification or mutation of a gene encoding a targetpolypeptide; (ii) mis-regulation of the gene encoding the targetpolypeptide; and (iii) aberrant post-translational modification of atarget polypeptide. Also, target polypeptides can be used as immunogensto produce anti-target antibodies in a subject, to purify targetpolypeptide ligands or binding partners, and in screening assays toidentify molecules which inhibit or enhance the interaction of a targetpolypeptide with a substrate.

In addition, polypeptides can be chemically synthesized using techniquesknown in the art (See, e.g., Creighton, 1983 Proteins. New York, N.Y.:W. H. Freeman and Company, and Hunkapiller et al., (1984) Nature July12-18; 310(5973):105-11). For example, a relative short fragment can besynthesized by use of a peptide synthesizer. Furthermore, if desired,non-classical amino acids or chemical amino acid analogs can beintroduced as a substitution or addition into the fragment sequence.Non-classical amino acids include, but are not limited to, to theD-isomers of the common amino acids, 2,4-diaminobutyric acid, a-aminoisobutyric acid, 4-aminobutyric acid, Abu, 2-amino butyric acid, g-Abu,e-Ahx, 6-amino hexanoic acid, Aib, 2-amino isobutyric acid, 3-aminopropionic acid, ornithine, norleucine, norvaline, hydroxyproline,sarcosine, citrulline, homocitrulline, cysteic acid, t-butylglycine,t-butylalanine, phenylglycine, cyclohexylalanine, b-alanine, fluoroaminoacids, designer amino acids such as b-methyl amino acids, Ca-methylamino acids, Na-methyl amino acids, and amino acid analogs in general.Furthermore, the amino acid can be D (dextrorotary) or L (levorotary).

Polypeptides and polypeptide fragments sometimes are differentiallymodified during or after translation, e.g., by glycosylation,acetylation, phosphorylation, amidation, derivatization by knownprotecting/blocking groups, proteolytic cleavage, linkage to an antibodymolecule or other cellular ligand, etc. Any of numerous chemicalmodifications may be carried out by known techniques, including but notlimited, to specific chemical cleavage by cyanogen bromide, trypsin,chymotrypsin, papain, V8 protease, NaBH4; acetylation, formylation,oxidation, reduction; metabolic synthesis in the presence oftunicamycin; and the like. Additional post-translational modificationsinclude, for example, N-linked or O-linked carbohydrate chains,processing of N-terminal or C-terminal ends), attachment of chemicalmoieties to the amino acid backbone, chemical modifications of N-linkedor O-linked carbohydrate chains, and addition or deletion of anN-terminal methionine residue as a result of prokaryotic host cellexpression. The polypeptide fragments may also be modified with adetectable label, such as an enzymatic, fluorescent, isotopic oraffinity label to allow for detection and isolation of the polypeptide.

Also provided are chemically modified derivatives of polypeptides thatcan provide additional advantages such as increased solubility,stability and circulating time of the polypeptide, or decreasedimmunogenicity (see e.g., U.S. Pat. No. 4,179,337. The chemical moietiesfor derivitization may be selected from water soluble polymers such aspolyethylene glycol, ethylene glycol/propylene glycol copolymers,carboxymethylcellulose, dextran, polyvinyl alcohol and the like. Thepolypeptides may be modified at random positions within the molecule, orat predetermined positions within the molecule and may include one, two,three or more attached chemical moieties.

The polymer may be of any molecular weight, and may be branched orunbranched. For polyethylene glycol, the preferred molecular weight isbetween about 1 kDa and about 100 kDa (the term “about” indicating thatin preparations of polyethylene glycol, some molecules will weigh more,some less, than the stated molecular weight) for ease in handling andmanufacturing. Other sizes may be used, depending on the desiredtherapeutic profile (e.g., the duration of sustained release desired,the effects, if any on biological activity, the ease in handling, thedegree or lack of antigenicity and other known effects of thepolyethylene glycol to a therapeutic protein or analog).

The polymers should be attached to the polypeptide with consideration ofeffects on functional or antigenic domains of the polypeptide. There area number of attachment methods available to those skilled in the art(e.g., EP 0 401 384 (coupling PEG to G-CSF) and Malik et al. (1992) ExpHematol. September; 20(8):1028-35 (pegylation of GM-CSF using tresylchloride)). For example, polyethylene glycol may be covalently boundthrough amino acid residues via a reactive group, such as a free aminoor carboxyl group. Reactive groups are those to which an activatedpolyethylene glycol molecule may be bound. The amino acid residueshaving a free amino group may include lysine residues and the N-terminalamino acid residues; those having a free carboxyl group may includeaspartic acid residues, glutamic acid residues and the C-terminal aminoacid residue. Sulfhydryl groups may also be used as a reactive group forattaching the polyethylene glycol molecules. For therapeutic purposes,the attachment sometimes is at an amino group, such as attachment at theN-terminus or lysine group.

Proteins can be chemically modified at the N-terminus. Usingpolyethylene glycol as an illustration of such a composition, one mayselect from a variety of polyethylene glycol molecules (by molecularweight, branching, and the like), the proportion of polyethylene glycolmolecules to protein (polypeptide) molecules in the reaction mix, thetype of pegylation reaction to be performed, and the method of obtainingthe selected N-terminally pegylated protein. The method of obtaining theN-terminally pegylated preparation (i.e., separating this moiety fromother monopegylated moieties if necessary) may be by purification of theN-terminally pegylated material from a population of pegylated proteinmolecules. Selective proteins chemically modified at the N-terminus maybe accomplished by reductive alkylation, which exploits differentialreactivity of different types of primary amino groups (lysine versus theN-terminal) available for derivatization in a particular protein. Underthe appropriate reaction conditions, substantially selectivederivatization of the protein at the N-terminus with a carbonyl groupcontaining polymer is achieved.

Substantially Identical Nucleic Acids and Polypeptides

Nucleotide sequences and polypeptide sequences that are substantiallyidentical to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotidesequence and the target polypeptide sequences encoded by thosenucleotide sequences, respectively, are included herein. The term“substantially identical” as used herein refers to two or more nucleicacids or polypeptides sharing one or more identical nucleotide sequencesor polypeptide sequences, respectively. Included are nucleotidesequences or polypeptide sequences that are 55% or more, 60% or more,65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% ormore, 95% or more (each often within a 1%, 2%, 3% or 4% variability)identical to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotidesequence or the encoded target polypeptide amino acid sequences. Onetest for determining whether two nucleic acids are substantiallyidentical is to determine the percent of identical nucleotide sequencesor polypeptide sequences shared between the nucleic acids orpolypeptides.

Calculations of sequence identity are often performed as follows.Sequences are aligned for optimal comparison purposes (e.g., gaps can beintroduced in one or both of a first and a second amino acid or nucleicacid sequence for optimal alignment and non-homologous sequences can bedisregarded for comparison purposes). The length of a reference sequencealigned for comparison purposes is sometimes 30% or more, 40% or more,50% or more, often 60% or more, and more often 70% or more, 80% or more,90% or more, or 100% of the length of the reference sequence. Thenucleotides or amino acids at corresponding nucleotide or polypeptidepositions, respectively, are then compared among the two sequences. Whena position in the first sequence is occupied by the same nucleotide oramino acid as the corresponding position in the second sequence, thenucleotides or amino acids are deemed to be identical at that position.The percent identity between the two sequences is a function of thenumber of identical positions shared by the sequences, taking intoaccount the number of gaps, and the length of each gap, introduced foroptimal alignment of the two sequences.

Comparison of sequences and determination of percent identity betweentwo sequences can be accomplished using a mathematical algorithm.Percent identity between two amino acid or nucleotide sequences can bedetermined using the algorithm of Meyers & Miller, CABIOS 4: 11-17(1989), which has been incorporated into the ALIGN program (version2.0), using a PAM120 weight residue table, a gap length penalty of 12and a gap penalty of 4. Also, percent identity between two amino acidsequences can be determined using the Needleman & Wunsch, J. Mol. Biol.48: 444-453 (1970) algorithm which has been incorporated into the GAPprogram in the GCG software package (available at the http addresswww.gcg.com), using either a Blossum 62 matrix or a PAM250 matrix, and agap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3,4, 5, or 6. Percent identity between two nucleotide sequences can bedetermined using the GAP program in the GCG software package (availableat http address www.gcg.com), using a NWSgapdna.CMP matrix and a gapweight of 40, 50, 60, 70, or 80 and a length weight of 1, 2, 3, 4, 5, or6. A set of parameters often used is a Blossum 62 scoring matrix with agap open penalty of 12, a gap extend penalty of 4, and a frameshift gappenalty of 5.

Another manner for determining if two nucleic acids are substantiallyidentical is to assess whether a polynucleotide homologous to onenucleic acid will hybridize to the other nucleic acid under stringentconditions. As use herein, the term “stringent conditions” refers toconditions for hybridization and washing. Stringent conditions are knownto those skilled in the art and can be found in Current Protocols inMolecular Biology, John Wiley & Sons, N.Y., 6.3.1-6.3.6 (1989). Aqueousand non-aqueous methods are described in that reference and either canbe used. An example of stringent hybridization conditions ishybridization in 6× sodium chloride/sodium citrate (SSC) at about 45°C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 50° C.Another example of stringent hybridization conditions are hybridizationin 6× sodium chloride/sodium citrate (SSC) at about 45° C., followed byone or more washes in 0.2×SSC, 0.1% SDS at 55° C. A further example ofstringent hybridization conditions is hybridization in 6× sodiumchloride/sodium citrate (SSC) at about 45° C., followed by one or morewashes in 0.2×SSC, 0.1% SDS at 60° C. Often, stringent hybridizationconditions are hybridization in 6× sodium chloride/sodium citrate (SSC)at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at65° C. More often, stringency conditions are 0.5M sodium phosphate, 7%SDS at 65° C., followed by one or more washes at 0.2×SSC, 1% SDS at 65°C.

An example of a substantially identical nucleotide sequence to anucleotide sequence in SEQ ID NO's:1-10 is one that has a differentnucleotide sequence but still encodes the same polypeptide sequenceencoded by the nucleotide sequence in SEQ ID NO's:1-10. Another exampleis a nucleotide sequence that encodes a polypeptide having a polypeptidesequence that is more than 70% or more identical to, sometimes more than75% or more, 80% or more, or 85% or more identical to, and often morethan 90% or more and 95% or more identical to a polypeptide sequenceencoded by a nucleotide sequence in SEQ ID NO's:1-10. As used herein,“SEQ ID NO's:1-10” typically refers to one or more sequences in SEQ IDNO: 1, 2, 3, 4, 5, 6, 7, 8, 9, and/or 10. Many of the embodimentsdescribed herein are applicable to (a) a nucleotide sequence of SEQ IDNO's:1-10; (b) a nucleotide sequence which encodes a polypeptideconsisting of an amino acid sequence encoded by a nucleotide sequence ofSEQ ID NO's:1-10; (c) a nucleotide sequence which encodes a polypeptidethat is 90% or more identical to an amino acid sequence encoded by anucleotide sequence of SEQ ID NO's:1-10, or a nucleotide sequence about90% or more identical to a nucleotide sequence of SEQ ID NO's:1-10; (d)a fragment of a nucleotide sequence of (a), (b), or (c); and/or anucleotide sequence complementary to the nucleotide sequences of (a),(b), (c) and/or (d), where nucleotide sequences of (b) and (c),fragments of (b) and (c) and nucleotide sequences complementary to (b)and (c) are examples of substantially identical nucleotide sequences.Examples of substantially identical nucleotide sequences includenucleotide sequences from subjects that differ by naturally occurringgenetic variance, which sometimes is referred to as background geneticvariance (e.g. nucleotide sequences differing by natural geneticvariance sometimes are 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or99% identical to one another).

Nucleotide sequences in SEQ ID NO's:1-10 and amino acid sequences ofencoded polypeptides can be used as “query sequences” to perform asearch against public databases to identify other family members orrelated sequences, for example. Such searches can be performed using theNBLAST and XBLAST programs (version 2.0) of Altschul et al., J. Mol.Biol. 215: 403-10 (1990). BLAST nucleotide searches can be performedwith the NBLAST program, score=100, wordlength=12 to obtain nucleotidesequences homologous to nucleotide sequences in SEQ ID NO's:1-10. BLASTpolypeptide searches can be performed with the XBLAST program, score=50,wordlength=3 to obtain amino acid sequences homologous to polypeptidesencoded by the nucleotide sequences of SEQ ID NO's:1-10. To obtaingapped alignments for comparison purposes, Gapped BLAST can be utilizedas described in Altschul et al., Nucleic Acids Res. 25(17): 3389-3402(1997). When utilizing BLAST and Gapped BLAST programs, defaultparameters of the respective programs (e.g., XBLAST and NBLAST) can beused (see the http address www.ncbi.nlm.nih.gov).

A nucleic acid that is substantially identical to a nucleotide sequencein SEQ ID NO's:1-10 may include polymorphic sites at positionsequivalent to those described herein when the sequences are aligned. Forexample, using the alignment procedures described herein, SNPs in asequence substantially identical to a sequence in SEQ ID NO's:1-10 canbe identified at nucleotide positions that match with or correspond to(i.e., align) nucleotides at SNP positions in each nucleotide sequencein SEQ ID NO's:1-10. Also, where a polymorphic variation results in aninsertion or deletion, insertion or deletion of a nucleotide sequencefrom a reference sequence can change the relative positions of otherpolymorphic sites in the nucleotide sequence.

Substantially identical nucleotide and polypeptide sequences includethose that are naturally occurring, such as allelic variants (samelocus), splice variants, homologs (different locus), and orthologs(different organism) or can be non-naturally occurring. Non-naturallyoccurring variants can be generated by mutagenesis techniques, includingthose applied to polynucleotides, cells, or organisms. The variants cancontain nucleotide substitutions, deletions, inversions and insertions.Variation can occur in either or both the coding and non-coding regions.The variations can produce both conservative and non-conservative aminoacid substitutions (as compared in the encoded product). Orthologs,homologs, allelic variants, and splice variants can be identified usingmethods known in the art. These variants normally comprise a nucleotidesequence encoding a polypeptide that is 50% or more, about 55% or more,often about 70-75% or more or about 80-85% or more, and sometimes about90-95% or more identical to the amino acid sequences of targetpolypeptides or a fragment thereof. Such nucleic acid molecules canreadily be identified as being able to hybridize under stringentconditions to a nucleotide sequence in SEQ ID NO's:1-10 or a fragment ofthis sequence. Nucleic acid molecules corresponding to orthologs,homologs, and allelic variants of a nucleotide sequence in SEQ IDNO's:1-10 can further be identified by mapping the sequence to the samechromosome or locus as the nucleotide sequence in SEQ ID NO's:1-10.

Also, substantially identical nucleotide sequences may include codonsthat are altered with respect to the naturally occurring sequence forenhancing expression of a target polypeptide in a particular expressionsystem. For example, the nucleic acid can be one in which one or morecodons are altered, and often 10% or more or 20% or more of the codonsare altered for optimized expression in bacteria (e.g., E. coli), yeast(e.g., S. cervesiae), human (e.g., 293 cells), insect, or rodent (e.g.,hamster) cells.

Methods for Identifying Subjects at Risk of Osteoporosis and Risk ofOsteoporosis in a Subject

Methods for prognosing and diagnosing low BMD and its related disorders(e.g., osteoporosis) are included herein. These methods includedetecting the presence or absence of one or more polymorphic variationsin a nucleotide sequence associated with low BMD, such as variants in oraround the loci set forth herein, or a substantially identical sequencethereof, in a sample from a subject, where the presence of a polymorphicvariant described herein is indicative of a risk of low BMD or one ormore low BMD related disorders (e.g., osteoporosis). Determining a riskof osteoporosis refers to determining whether an individual is at anincreased risk of osteoporosis (e.g., intermediate risk or higher risk).

Thus, featured herein is a method for identifying a subject who is atrisk of osteoporosis, which comprises detecting low BMD-associatedaberration in a nucleic acid sample from the subject. An embodiment is amethod for detecting a risk of osteoporosis in a subject, whichcomprises detecting the presence or absence of a polymorphic variationassociated with low BMD at a polymorphic site in a nucleotide sequencein a nucleic acid sample from a subject, where the nucleotide sequencecomprises a polynucleotide sequence selected from the group consistingof: (a) a nucleotide sequence of SEQ ID NO's:1-10; (b) a nucleotidesequence which encodes a polypeptide consisting of an amino acidsequence encoded by a nucleotide sequence of SEQ ID NO's:1-10; (c) anucleotide sequence which encodes a polypeptide that is 90% or moreidentical to an amino acid sequence encoded by a nucleotide sequence ofSEQ ID NO's:1-10, or a nucleotide sequence about 90% or more identicalto a nucleotide sequence of SEQ ID NO's:1-10; and (d) a fragment of anucleotide sequence of (a), (b), or (c) comprising the polymorphic site;whereby the presence of the polymorphic variation is indicative of apredisposition to osteoporosis in the subject. In certain embodiments,polymorphic variants at the positions described herein are detected fordetermining a risk of osteoporosis, and polymorphic variants atpositions in linkage disequilibrium with these positions are detectedfor determining a risk of osteoporosis. As used herein, “SEQ IDNO's:1-10” refers to individual sequences in SEQ ID NO: 1, 2, 3, 4, 5,6, 7, 8, 9, 10, 11, and/or 12.

Results from prognostic tests may be combined with other test results todiagnose osteoporosis. For example, prognostic results may be gathered,an initial diagnostic test may be ordered based on a determinedpredisposition to low BMD, and the results of the analysis may beutilized to diagnose osteoporosis. Also osteoporosis diagnostic methodscan be developed from studies used to generate prognostic/diagnosticmethods in which populations are stratified into subpopulations havingdifferent progressions of osteoporosis. In another embodiment,prognostic results may be gathered; a patient's risk factors fordeveloping osteoporosis analyzed (e.g., age, race, family history, ageof menopause); and an initial diagnostic test may be ordered based on adetermined predisposition to low BMD.

Risk factors believed to be associated with low BMD include personalhistory of fracture after age 50; current low bone mass; history offracture in a 1° relative; being female; being thin and/or having asmall frame; low body weight; advanced age; a family history ofosteoporosis; estrogen deficiency, especially as a result of menopausewhich is early or surgically induced; abnormal absence of menstrualperiods (amenorrhea); anorexia nervosa; low lifetime calcium intake; useof certain medications, such as corticosteroids and anticonvulsants; lowtestosterone levels in men; an inactive lifestyle; current cigarettesmoking; excessive use of alcohol; being Caucasian or Asian, althoughAfrican Americans and Hispanic Americans are at significant risk aswell. (See National Osteoporosis Foundation;http://www.nof.org/osteoporosis/stats.htm). In an alternativeembodiment, the results from predisposition analyses described hereinmay be combined with other test results indicative of osteoporosis,which were previously, concurrently, or subsequently gathered withrespect to the predisposition testing. In these embodiments, thecombination of the prognostic test results with other test results canbe probative of osteoporosis, and the combination can be utilized as anosteoporosis diagnostic. The results of any test indicative ofosteoporosis known in the art may be combined with the methods describedherein. Examples of such tests are bone density tests that measure bonedensity in various sites of the body. Such methods include themeasurement of the radiodensity of skeletal radiographs, quantitativecomputerized tomography, single energy photon absorptiometry, anddual-energy photon absorptiometry. Diagnostic techniques among thoseuseful herein are described in W. A. Peck et al., Physician's ResourceManual on Osteoporosis (1987), published by the National OsteoporosisFoundation (incorporated by reference herein).

Risk of low BMD sometimes is expressed as a probability, such as an oddsratio, percentage, or risk factor. The risk sometimes is expressed as arelative risk with respect to a population average risk of low BMD, andsometimes is expressed as a relative risk with respect to the lowestrisk group. Such relative risk assessments often are based uponpenetrance values determined by statistical methods and are particularlyuseful to clinicians and insurance companies for assessing risk ofosteoporosis (e.g., a clinician can target appropriate detection,prevention and therapeutic regimens to a patient after determining thepatient's risk of osteoporosis, and an insurance company can fine tuneactuarial tables based upon population genotype assessments ofosteoporosis risk). Risk of osteoporosis sometimes is expressed as anodds ratio, which is the odds of a particular person having a genotypehas or will develop osteoporosis with respect to another genotype group(e.g., the most disease protective genotype or population average). Therisk often is based upon the presence or absence of one or morepolymorphic variants described herein, and also may be based in partupon phenotypic traits of the individual being tested. In an embodiment,two or more polymorphic variations are detected in a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 locus. In certain embodiments, 3 or more, or 4, 5,6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 or morepolymorphic variants are detected in the sample. Methods for calculatingrisk based upon patient data are well known (see, e.g., Agresti,Categorical Data Analysis, 2nd Ed. 2002. Wiley). Allelotyping andgenotyping analyses may be carried out in populations other than thoseexemplified herein to enhance the predictive power of the prognosticmethod.

The nucleic acid sample typically is isolated from a biological sampleobtained from a subject. For example, nucleic acid can be isolated fromblood, saliva, sputum, urine, cell scrapings, and biopsy tissue. Thenucleic acid sample can be isolated from a biological sample usingstandard techniques, such as the technique described in Example 2. Asused herein, the term “subject” refers primarily to humans but alsorefers to other mammals such as dogs, cats, and ungulates (e.g., cattle,sheep, and swine). Subjects also include avians (e.g., chickens andturkeys), reptiles, and fish (e.g., salmon), as embodiments describedherein can be adapted to nucleic acid samples isolated from any of theseorganisms. The nucleic acid sample may be isolated from the subject andthen directly utilized in a method for determining the presence of apolymorphic variant, or alternatively, the sample may be isolated andthen stored (e.g., frozen) for a period of time before being subjectedto analysis.

The presence or absence of a polymorphic variant is determined using oneor both chromosomal complements represented in the nucleic acid sample.Determining the presence or absence of a polymorphic variant in bothchromosomal complements represented in a nucleic acid sample from asubject having a copy of each chromosome is useful for determining thezygosity of an individual for the polymorphic variant (i.e., whether theindividual is homozygous or heterozygous for the polymorphic variant).Any oligonucleotide-based diagnostic may be utilized to determinewhether a sample includes the presence or absence of a polymorphicvariant in a sample. For example, primer extension methods, ligasesequence determination methods (e.g., U.S. Pat. Nos. 5,679,524 and5,952,174, and WO 01/27326), mismatch sequence determination methods(e.g., U.S. Pat. Nos. 5,851,770; 5,958,692; 6,110,684; and 6,183,958),microarray sequence determination methods, restriction fragment lengthpolymorphism (RFLP), single strand conformation polymorphism detection(SSCP) (e.g., U.S. Pat. Nos. 5,891,625 and 6,013,499), PCR-based assays(e.g., TAQMAN® PCR System (Applied Biosystems)), and nucleotidesequencing methods may be used.

Oligonucleotide extension methods typically involve providing a pair ofoligonucleotide primers in a polymerase chain reaction (PCR) or in othernucleic acid amplification methods for the purpose of amplifying aregion from the nucleic acid sample that comprises the polymorphicvariation. One oligonucleotide primer is complementary to a region 3′ ofthe polymorphism and the other is complementary to a region 5′ of thepolymorphism. A PCR primer pair may be used in methods disclosed in U.S.Pat. Nos. 4,683,195; 4,683,202, 4,965,188; 5,656,493; 5,998,143;6,140,054; WO 01/27327; and WO 01/27329 for example. PCR primer pairsmay also be used in any commercially available machines that performPCR, such as any of the GENEAMP® Systems available from AppliedBiosystems. Also, those of ordinary skill in the art will be able todesign oligonucleotide primers based upon a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence using knowledge available in the art.

Also provided is an extension oligonucleotide that hybridizes to theamplified fragment adjacent to the polymorphic variation. As usedherein, the term “adjacent” refers to the 3′ end of the extensionoligonucleotide being often 1 nucleotide from the 5′ end of thepolymorphic site, and sometimes 2, 3, 4, 5, 6, 7, 8, 9, or 10nucleotides from the 5′ end of the polymorphic site, in the nucleic acidwhen the extension oligonucleotide is hybridized to the nucleic acid.The extension oligonucleotide then is extended by one or morenucleotides, and the number and/or type of nucleotides that are added tothe extension oligonucleotide determine whether the polymorphic variantis present. Oligonucleotide extension methods are disclosed, forexample, in U.S. Pat. Nos. 4,656,127; 4,851,331; 5,679,524; 5,834,189;5,876,934; 5,908,755; 5,912,118; 5,976,802; 5,981,186; 6,004,744;6,013,431; 6,017,702; 6,046,005; 6,087,095; 6,210,891; and WO 01/20039.Oligonucleotide extension methods using mass spectrometry are described,for example, in U.S. Pat. Nos. 5,547,835; 5,605,798; 5,691,141;5,849,542; 5,869,242; 5,928,906; 6,043,031; and 6,194,144, and a methodoften utilized is described herein in Example 2.

A microarray can be utilized for determining whether a polymorphicvariant is present or absent in a nucleic acid sample. A microarray mayinclude any oligonucleotides described herein, and methods for makingand using oligonucleotide microarrays suitable for diagnostic use aredisclosed in U.S. Pat. Nos. 5,492,806; 5,525,464; 5,589,330; 5,695,940;5,849,483; 6,018,041; 6,045,996; 6,136,541; 6,142,681; 6,156,501;6,197,506; 6,223,127; 6,225,625; 6,229,911; 6,239,273; WO 00/52625; WO01/25485; and WO 01/29259. The microarray typically comprises a solidsupport and the oligonucleotides may be linked to this solid support bycovalent bonds or by non-covalent interactions. The oligonucleotides mayalso be linked to the solid support directly or by a spacer molecule. Amicroarray may comprise one or more oligonucleotides complementary to apolymorphic site set forth herein.

A kit also may be utilized for determining whether a polymorphic variantis present or absent in a nucleic acid sample. A kit often comprises oneor more pairs of oligonucleotide primers useful for amplifying afragment of a nucleotide sequence of SEQ ID NO's:1-10 or a substantiallyidentical sequence thereof, where the fragment includes a polymorphicsite. The kit sometimes comprises a polymerizing agent, for example, athermostable nucleic acid polymerase such as one disclosed in U.S. Pat.No. 4,889,818 or 6,077,664. Also, the kit often comprises an elongationoligonucleotide that hybridizes to a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence in a nucleic acid sample adjacent to thepolymorphic site. Where the kit includes an elongation oligonucleotide,it also often comprises chain elongating nucleotides, such as dATP,dTTP, dGTP, dCTP, and dITP, including analogs of dATP, dTTP, dGTP, dCTPand dITP, provided that such analogs are substrates for a thermostablenucleic acid polymerase and can be incorporated into a nucleic acidchain elongated from the extension oligonucleotide. Along with chainelongating nucleotides would be one or more chain terminatingnucleotides such as ddATP, ddTTP, ddGTP, ddCTP, and the like. In anembodiment, the kit comprises one or more oligonucleotide primer pairs,a polymerizing agent, chain elongating nucleotides, at least oneelongation oligonucleotide, and one or more chain terminatingnucleotides. Kits optionally include buffers, vials, microtiter plates,and instructions for use.

An individual identified as being at risk of osteoporosis may beheterozygous or homozygous with respect to the allele associated withlow BMD. A subject homozygous for an allele associated with low BMD isat a comparatively high risk of osteoporosis, a subject heterozygous foran allele associated with low BMD is at a comparatively intermediaterisk of osteoporosis, and a subject homozygous for an allele associatedwith normal or high BMD levels (i.e., a decreased risk of low BMD) is ata comparatively low risk of osteoporosis. A genotype may be assessed fora complementary strand, such that the complementary nucleotide at aparticular position is detected.

Also featured are methods for determining risk of osteoporosis and/oridentifying a subject at risk of osteoporosis by contacting apolypeptide or protein encoded by a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence from a subject with an antibody thatspecifically binds to an epitope associated with increased risk ofosteoporosis in the polypeptide. In an embodiment, the antibodyspecifically binds to an epitope comprising an arginine at position 120in a PROL4 polypeptide.

Applications of Prognostic and Diagnostic Results to PharmacogenomicMethods

Pharmacogenomics is a discipline that involves tailoring a treatment fora subject according to the subject's genotype as a particular treatmentregimen may exert a differential effect depending upon the subject'sgenotype. For example, based upon the outcome of a prognostic testdescribed herein, a clinician or physician may target pertinentinformation and preventative or therapeutic treatments to a subject whowould be benefited by the information or treatment and avoid directingsuch information and treatments to a subject who would not be benefited(e.g., the treatment has no therapeutic effect and/or the subjectexperiences adverse side effects).

The following is an example of a pharmacogenomic embodiment. Aparticular treatment regimen can exert a differential effect dependingupon the subject's genotype. Where a candidate therapeutic exhibits asignificant interaction with a major allele and a comparatively weakinteraction with a minor allele (e.g., an order of magnitude or greaterdifference in the interaction), such a therapeutic typically would notbe administered to a subject genotyped as being homozygous for the minorallele, and sometimes not administered to a subject genotyped as beingheterozygous for the minor allele. In another example, where a candidatetherapeutic is not significantly toxic when administered to subjects whoare homozygous for a major allele but is comparatively toxic whenadministered to subjects heterozygous or homozygous for a minor allele,the candidate therapeutic is not typically administered to subjects whoare genotyped as being heterozygous or homozygous with respect to theminor allele.

The methods described herein are applicable to pharmacogenomic methodsfor preventing, alleviating or treating low BMD conditions such asosteoporosis. For example, a nucleic acid sample from an individual maybe subjected to a prognostic test described herein. Where one or morepolymorphic variations associated with low BMD are identified in asubject, information for preventing or treating osteoporosis and/or oneor more osteoporosis treatment regimens then may be prescribed to thatsubject.

In certain embodiments, a treatment or preventative regimen isspecifically prescribed and/or administered to individuals who will mostbenefit from it based upon their risk of developing osteoporosisassessed by the methods described herein. Thus, provided are methods foridentifying a subject predisposed to osteoporosis and then prescribing atherapeutic or preventative regimen to individuals identified as havinga predisposition. Thus, certain embodiments are directed to a method forincreasing BMD levels or otherwise treating osteoporosis in a subject,which comprises: detecting the presence or absence of a polymorphicvariant associated with low BMD in a nucleotide sequence in a nucleicacid sample from a subject, where the nucleotide sequence comprises apolynucleotide sequence selected from the group consisting of: (a) anucleotide sequence of SEQ ID NO's:1-10; (b) a nucleotide sequence whichencodes a polypeptide consisting of an amino acid sequence encoded by anucleotide sequence of SEQ ID NO's:1-10; (c) a nucleotide sequence whichencodes a polypeptide that is 90% or more identical to an amino acidsequence encoded by a nucleotide sequence of SEQ ID NO's:1-10, or anucleotide sequence about 90% or more identical to a nucleotide sequenceof SEQ ID NO's:1-10; and (d) a fragment of a polynucleotide sequence of(a), (b), or (c); and prescribing or administering a treatment regimento a subject from whom the sample originated where the presence of apolymorphic variation associated with low BMD is detected in thenucleotide sequence. In these methods, predisposition results may beutilized in combination with other test results to diagnose low BMDassociated conditions, such as osteoporosis.

Certain preventative treatments often are prescribed to subjects havinga predisposition to osteoporosis and where the subject is diagnosed withlow BMD or is diagnosed as having symptoms indicative of early stageosteoporosis. Established diagnostic techniques use x-ray andultrasonography to measure skeletal parameters of bone size, volume andmineral density to predict fracture risk and to assess response totherapy. Such measurements give a “static” value which can be comparedto normal values to aid diagnosis of low bone mass and fracture risk(Schott, Cornier et al. 1998). The World Health Organization definesosteoporosis as a bone mineral density level more than 2.5 standarddeviations below the young normal mean. The various techniques used tomeasure bone mineral density include, a) Dual energy X-rayabsorptiometry (DXA)—used to measure bone mass at the lumbar spine andhip, but it can also be applied to measuring total skeletal bone mass,soft-tissue composition and other regional bone measurements. DXA isconsidered the “gold standard” for BMD measurement; b) high-resolutionquantitative computed tomography QCT)—highly sensitive, accurate andspecific spinal measurements. This technique is more costly, not widelyavailable and involves higher radiation doses than other techniques; c)single-energy x-ray absorptiometry (SXA)—provides accurate radius BMDmeasurements; d) quantitative ultrasound QUS)—new and promisingtechnique which may have applications in both BMD measurement andassessment or architectural deterioration of bone tissue. Recent studiessuggest QUS of calcaneus bone predicts hip fracture as well as DXA(Hans, Dargent-Molina et al. 1996).

An alternative method to predict fracture independently of bone mass isto measure bone turnover. High turnover (bone resorption and formation)is associated with rapid bone loss and is likely to contribute tomicro-architectural deterioration (Ross and Knowlton 1998). This is a“dynamic” measurement which is assessed with biochemical markers inurine or serum and can be used effectively in therapy monitoring inpreference to BNO measurements. When used in combination with bone massassessment, biomarkers can provide more accurate fracture predictionsover bone mass measurement alone. Osteoporosis-related biomarkers forbone resorption include deoxypyridinoline crosslinks, andosteoporosis-related biomarkers for bone formation include bone alkalinephosphatase and osteocalcin. Some of these biomarkers have beendeveloped for use in diagnostic kits. The current challenge is to reducethe variability of the measurements and improve their reliability andapplicability.

The treatment sometimes is preventative (e.g., is prescribed oradministered to reduce the probability that a low BMD associatedcondition arises or progresses), sometimes is therapeutic, and sometimesdelays, alleviates or halts the progression of a low BMD associatedcondition. Any known preventative or therapeutic treatment foralleviating or preventing the occurrence of a low BMD associateddisorder is prescribed and/or administered. Preventative treatment forosteoporosis is most effective at the time when bone loss is increasingand before the bones have become fragile and prone to fracturing.Strategies for the prevention of this disease include development ofbone density in early adulthood (i.e., building strong bones duringchildhood and adolescence), and minimization of bone loss in later life.Changes in lifestyle, nutrition and hormonal factors have been shown toaffect bone loss. Specifically, a balanced diet rich in calcium andvitamin D, weight-bearing exercise, a healthy lifestyle with no smokingor excessive alcohol intake, and bone density testing and medicationwhen appropriate are known to help reduce the risk of osteoporosis.

As therapeutic approaches for low BMD continue to evolve and improve,the goal of treatments for low BMD related disorders is to interveneeven before clinical signs (e.g., impaired glucose tolerance, or IGT)first manifest. Thus, genetic markers associated with susceptibility tolow BMD prove useful for early diagnosis, prevention and treatment oflow BMD.

As osteoporosis preventative and treatment information can bespecifically targeted to subjects in need thereof (e.g., those at riskof low BMD or those that have early stages of osteoporosis), providedherein is a method for preventing or reducing the risk of developingosteoporosis in a subject, which comprises: (a) detecting the presenceor absence of a polymorphic variation associated with low BMD at apolymorphic site in a nucleotide sequence in a nucleic acid sample froma subject; (b) identifying a subject with a predisposition toosteoporosis, whereby the presence of the polymorphic variation isindicative of a predisposition to osteoporosis in the subject; and (c)if such a predisposition is identified, providing the subject withinformation about methods or products to prevent osteoporosis or todelay the onset of osteoporosis. Also provided is a method of targetinginformation or advertising to a subpopulation of a human populationbased on the subpopulation being genetically predisposed to a disease orcondition, which comprises: (a) detecting the presence or absence of apolymorphic variation associated with low BMD at a polymorphic site in anucleotide sequence in a nucleic acid sample from a subject; (b)identifying the subpopulation of subjects in which the polymorphicvariation is associated with low BMD; and (c) providing information onlyto the subpopulation of subjects about a particular product which may beobtained and consumed or applied by the subject to help prevent or delayonset of the disease or condition.

Pharmacogenomics methods also may be used to analyze and predict aresponse to a osteoporosis treatment or a drug. For example, ifpharmacogenomics analysis indicates a likelihood that an individual willrespond positively to a osteoporosis treatment with a particular drug,the drug may be administered to the individual. Conversely, if theanalysis indicates that an individual is likely to respond negatively totreatment with a particular drug, an alternative course of treatment maybe prescribed. A negative response may be defined as either the absenceof an efficacious response or the presence of toxic side effects. Theresponse to a therapeutic treatment can be predicted in a backgroundstudy in which subjects in any of the following populations aregenotyped: a population that responds favorably to a treatment regimen,a population that does not respond significantly to a treatment regimen,and a population that responds adversely to a treatment regiment (e.g.,exhibits one or more side effects). These populations are provided asexamples and other populations and subpopulations may be analyzed. Basedupon the results of these analyses, a subject is genotyped to predictwhether he or she will respond favorably to a treatment regimen, notrespond significantly to a treatment regimen, or respond adversely to atreatment regimen.

The tests described herein also are applicable to clinical drug trials.One or more polymorphic variants indicative of response to an agent fortreating osteoporosis or to side effects to an agent for treatingosteoporosis may be identified using the methods described herein.Thereafter, potential participants in clinical trials of such an agentmay be screened to identify those individuals most likely to respondfavorably to the drug and exclude those likely to experience sideeffects. In that way, the effectiveness of drug treatment may bemeasured in individuals who respond positively to the drug, withoutlowering the measurement as a result of the inclusion of individuals whoare unlikely to respond positively in the study and without riskingundesirable safety problems.

Thus, another embodiment is a method of selecting an individual forinclusion in a clinical trial of a treatment or drug comprising thesteps of: (a) obtaining a nucleic acid sample from an individual; (b)determining the identity of a polymorphic variation which is associatedwith a positive response to the treatment or the drug, or at least onepolymorphic variation which is associated with a negative response tothe treatment or the drug in the nucleic acid sample, and (c) includingthe individual in the clinical trial if the nucleic acid sample containssaid polymorphic variation associated with a positive response to thetreatment or the drug or if the nucleic acid sample lacks saidpolymorphic variation associated with a negative response to thetreatment or the drug. In addition, the methods described herein forselecting an individual for inclusion in a clinical trial of a treatmentor drug encompass methods with any further limitation described in thisdisclosure, or those following, specified alone or in any combination.The polymorphic variation may be in a sequence selected individually orin any combination from the group consisting of (i) a nucleotidesequence of SEQ ID NO's:1-10; (ii) a nucleotide sequence which encodes apolypeptide consisting of an amino acid sequence encoded by a nucleotidesequence of SEQ ID NO's:1-10; (iii) a nucleotide sequence which encodesa polypeptide that is 90% or more identical to an amino acid sequenceencoded by a nucleotide sequence of SEQ ID NO's:1-10, or a nucleotidesequence about 90% or more identical to a nucleotide sequence of SEQ IDNO's:1-10; and (iv) a fragment of a polynucleotide sequence of (i),(ii), or (iii) comprising the polymorphic site. The including step (c)optionally comprises administering the drug or the treatment to theindividual if the nucleic acid sample contains the polymorphic variationassociated with a positive response to the treatment or the drug and thenucleic acid sample lacks said biallelic marker associated with anegative response to the treatment or the drug.

Also provided herein is a method of partnering between adiagnostic/prognostic testing provider and a provider of a consumableproduct, which comprises: (a) the diagnostic/prognostic testing providerdetects the presence or absence of a polymorphic variation associatedwith low BMD at a polymorphic site in a nucleotide sequence in a nucleicacid sample from a subject; (b) the diagnostic/prognostic testingprovider identifies the subpopulation of subjects in which thepolymorphic variation is associated with low BMD; (c) thediagnostic/prognostic testing provider forwards information to thesubpopulation of subjects about a particular product which may beobtained and consumed or applied by the subject to help prevent or delayonset of the disease or condition; and (d) the provider of a consumableproduct forwards to the diagnostic test provider a fee every time thediagnostic/prognostic test provider forwards information to the subjectas set forth in step (c) above.

Compositions Comprising Osteoporosis-Directed Molecules

Featured herein is a composition comprising a cell from a subject havinglow BMD or at risk of osteoporosis and one or more moleculesspecifically directed and targeted to a nucleic acid comprising a CETP,PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or amino acidsequence. Such directed molecules include, but are not limited to, acompound that binds to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleotide sequence or amino acid sequence referenced herein; a nucleicacid that hybridizes to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleic acid under conditions of high stringency; a RNAi or siRNAmolecule having a strand complementary to a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence; an antisense nucleic acid complementaryto an RNA encoded by a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleotide sequence; a ribozyme that hybridizes to a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleotide sequence; a nucleic acid aptamer thatspecifically binds a polypeptide encoded by CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence; and an antibody that specifically bindsto a polypeptide encoded by CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1nucleotide sequence or binds to a nucleic acid having such a nucleotidesequence. In specific embodiments, the osteoporosis directed moleculeinteracts with a nucleic acid or polypeptide variant associated withosteoporosis, such as variants referenced herein. In other embodiments,the osteoporosis directed molecule interacts with a polypeptide involvedin a signal pathway of a polypeptide encoded by a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleotide sequence, or a nucleic acid comprisingsuch a nucleotide sequence.

Compositions sometimes include an adjuvant known to stimulate an immuneresponse, and in certain embodiments, an adjuvant that stimulates aT-cell lymphocyte response. Adjuvants are known, including but notlimited to an aluminum adjuvant (e.g., aluminum hydroxide); a cytokineadjuvant or adjuvant that stimulates a cytokine response (e.g.,interleukin (IL)-12 and/or γ-interferon cytokines); a Freund-typemineral oil adjuvant emulsion (e.g., Freund's complete or incompleteadjuvant); a synthetic lipoid compound; a copolymer adjuvant (e.g.,TitreMax); a saponin; Quil A; a liposome; an oil-in-water emulsion(e.g., an emulsion stabilized by Tween 80 and pluronicpolyoxyethlene/polyoxypropylene block copolymer (Syntex AdjuvantFormulation); TitreMax; detoxified endotoxin (MPL) and mycobacterialcell wall components (TDW, CWS) in 2% squalene (Ribi Adjuvant System));a muramyl dipeptide; an immune-stimulating complex (ISCOM, e.g., anAg-modified saponin/cholesterol micelle that forms stable cage-likestructure); an aqueous phase adjuvant that does not have a depot effect(e.g., Gerbu adjuvant); a carbohydrate polymer (e.g., AdjuPrime);L-tyrosine; a manide-oleate compound (e.g., Montanide); anethylene-vinyl acetate copolymer (e.g., Elvax 40W1,2); or lipid A, forexample. Such compositions are useful for generating an immune responseagainst a osteoporosis directed molecule (e.g., an HLA-bindingsubsequence within a polypeptide encoded by a CETP, PROL4, GRID2, PDE4Dor GPX3/TNIP1 nucleotide sequence). In such methods, a peptide having anamino acid subsequence of a polypeptide encoded by a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleotide sequence is delivered to a subject, wherethe subsequence binds to an HLA molecule and induces a CTL lymphocyteresponse. The peptide sometimes is delivered to the subject as anisolated peptide or as a minigene in a plasmid that encodes the peptide.Methods for identifying HLA-binding subsequences in such polypeptidesare known (see e.g., publication WO02/20616 and PCT applicationUS98/01373 for methods of identifying such sequences).

The cell may be in a group of cells cultured in vitro or in a tissuemaintained in vitro or present in an animal in vivo (e.g., a rat, mouse,ape or human). In certain embodiments, a composition comprises acomponent from a cell such as a nucleic acid molecule (e.g., genomicDNA), a protein mixture or isolated protein, for example. Theaforementioned compositions have utility in diagnostic, prognostic andpharmacogenomic methods described previously and in osteoporosistherapeutics described hereafter. Certain osteoporosis directedmolecules are described in greater detail below.

Compounds

Compounds can be obtained using any of the numerous approaches incombinatorial library methods known in the art, including: biologicallibraries; peptoid libraries (libraries of molecules having thefunctionalities of peptides, but with a novel, non-peptide backbonewhich are resistant to enzymatic degradation but which neverthelessremain bioactive (see, e.g., Zuckermann et al., J. Med. Chem. 37:2678-85 (1994)); spatially addressable parallel solid phase or solutionphase libraries; synthetic library methods requiring deconvolution;“one-bead one-compound” library methods; and synthetic library methodsusing affinity chromatography selection. Biological library and peptoidlibrary approaches are typically limited to peptide libraries, while theother approaches are applicable to peptide, non-peptide oligomer orsmall molecule libraries of compounds (Lam, Anticancer Drug Des. 12:145, (1997)). Examples of methods for synthesizing molecular librariesare described, for example, in DeWitt et al., Proc. Natl. Acad. Sci.U.S.A. 90: 6909 (1993); Erb et al., Proc. Natl. Acad. Sci. USA 91: 11422(1994); Zuckermann et al., J. Med. Chem. 37: 2678 (1994); Cho et al.,Science 261: 1303 (1993); Carrell et al., Angew. Chem. Int. Ed. Engl.33: 2059 (1994); Carell et al., Angew. Chem. Int. Ed. Engl. 33: 2061(1994); and in Gallop et al., J. Med. Chem. 37: 1233 (1994).

Libraries of compounds may be presented in solution (e.g., Houghten,Biotechniques 13: 412-421 (1992)), or on beads (Lam, Nature 354: 82-84(1991)), chips (Fodor, Nature 364: 555-556 (1993)), bacteria or spores(Ladner, U.S. Pat. No. 5,223,409), plasmids (Cull et al., Proc. Natl.Acad. Sci. USA 89: 1865-1869 (1992)) or on phage (Scott and Smith,Science 249: 386-390 (1990); Devlin, Science 249: 404-406 (1990); Cwirlaet al., Proc. Natl. Acad. Sci. 87: 6378-6382 (1990); Felici, J. Mol.Biol. 222: 301-310 (1991); Ladner supra.).

A compound sometimes alters expression and sometimes alters activity ofa polypeptide target and may be a small molecule. Small moleculesinclude, but are not limited to, peptides, peptidomimetics (e.g.,peptoids), amino acids, amino acid analogs, polynucleotides,polynucleotide analogs, nucleotides, nucleotide analogs, organic orinorganic compounds (i.e., including heteroorganic and organometalliccompounds) having a molecular weight less than about 10,000 grams permole, organic or inorganic compounds having a molecular weight less thanabout 5,000 grams per mole, organic or inorganic compounds having amolecular weight less than about 1,000 grams per mole, organic orinorganic compounds having a molecular weight less than about 500 gramsper mole, and salts, esters, and other pharmaceutically acceptable formsof such compounds.

Antisense Nucleic Acid Molecules, Ribozymes, RNAi, siRNA and ModifiedNucleic Acid Molecules

An “antisense” nucleic acid refers to a nucleotide sequencecomplementary to a “sense” nucleic acid encoding a polypeptide, e.g.,complementary to the coding strand of a double-stranded cDNA molecule orcomplementary to an mRNA sequence. The antisense nucleic acid can becomplementary to an entire coding strand (e.g., SEQ ID NO: 7-13), or toa portion thereof or a substantially identical sequence thereof. Inanother embodiment, the antisense nucleic acid molecule is antisense toa “noncoding region” of the coding strand of a nucleotide sequence(e.g., 5′ and 3′ untranslated regions in SEQ ID NO's:1-5).

An antisense nucleic acid can be designed such that it is complementaryto the entire coding region of an mRNA encoded by a nucleotide sequence(e.g., SEQ ID NO's:1-10), and often the antisense nucleic acid is anoligonucleotide antisense to only a portion of a coding or noncodingregion of the mRNA. For example, the antisense oligonucleotide can becomplementary to the region surrounding the translation start site ofthe mRNA, e.g., between the −10 and +10 regions of the target genenucleotide sequence of interest. An antisense oligonucleotide can be,for example, about 7, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, or more nucleotides in length. The antisense nucleic acids,which include the ribozymes described hereafter, can be designed totarget a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence,often a variant associated with low BMD, or a substantially identicalsequence thereof. Among the variants, minor alleles and major allelescan be targeted, and those associated with a higher risk of osteoporosisare often designed, tested, and administered to subjects.

An antisense nucleic acid can be constructed using chemical synthesisand enzymatic ligation reactions using standard procedures. For example,an antisense nucleic acid (e.g., an antisense oligonucleotide) can bechemically synthesized using naturally occurring nucleotides orvariously modified nucleotides designed to increase the biologicalstability of the molecules or to increase the physical stability of theduplex formed between the antisense and sense nucleic acids, e.g.,phosphorothioate derivatives and acridine substituted nucleotides can beused. Antisense nucleic acid also can be produced biologically using anexpression vector into which a nucleic acid has been subcloned in anantisense orientation (i.e., RNA transcribed from the inserted nucleicacid will be of an antisense orientation to a target nucleic acid ofinterest, described further in the following subsection).

When utilized as therapeutics, antisense nucleic acids typically areadministered to a subject (e.g., by direct injection at a tissue site)or generated in situ such that they hybridize with or bind to cellularmRNA and/or genomic DNA encoding a polypeptide and thereby inhibitexpression of the polypeptide, for example, by inhibiting transcriptionand/or translation. Alternatively, antisense nucleic acid molecules canbe modified to target selected cells and then are administeredsystemically. For systemic administration, antisense molecules can bemodified such that they specifically bind to receptors or antigensexpressed on a selected cell surface, for example, by linking antisensenucleic acid molecules to peptides or antibodies which bind to cellsurface receptors or antigens. Antisense nucleic acid molecules can alsobe delivered to cells using the vectors described herein. Sufficientintracellular concentrations of antisense molecules are achieved byincorporating a strong promoter, such as a pol II or pol III promoter,in the vector construct.

Antisense nucleic acid molecules sometimes are *-anomeric nucleic acidmolecules. An *-anomeric nucleic acid molecule forms specificdouble-stranded hybrids with complementary RNA in which, contrary to theusual *-units, the strands run parallel to each other (Gaultier et al.,Nucleic Acids. Res. 15: 6625-6641 (1987)). Antisense nucleic acidmolecules can also comprise a 2′-o-methylribonucleotide (Inoue et al.,Nucleic Acids Res. 15: 6131-6148 (1987)) or a chimeric RNA-DNA analogue(Inoue et al., FEBS Lett. 215: 327-330 (1987)). Antisense nucleic acidssometimes are composed of DNA or PNA or any other nucleic acidderivatives described previously.

In another embodiment, an antisense nucleic acid is a ribozyme. Aribozyme having specificity for a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence can include one or more sequencescomplementary to such a nucleotide sequence, and a sequence having aknown catalytic region responsible for mRNA cleavage (see e.g., U.S.Pat. No. 5,093,246 or Haselhoff and Gerlach, Nature 334: 585-591(1988)). For example, a derivative of a Tetrahymena L-19 IVS RNA issometimes utilized in which the nucleotide sequence of the active siteis complementary to the nucleotide sequence to be cleaved in a mRNA (seee.g., Cech et al. U.S. Pat. No. 4,987,071; and Cech et al. U.S. Pat. No.5,116,742). Also, target mRNA sequences can be used to select acatalytic RNA having a specific ribonuclease activity from a pool of RNAmolecules (see e.g., Bartel & Szostak, Science 261: 1411-1418(1993)).

Osteoporosis directed molecules include in certain embodiments nucleicacids that can form triple helix structures with a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleotide sequence or a substantially identicalsequence thereof, especially one that includes a regulatory region thatcontrols expression of a polypeptide. Gene expression can be inhibitedby targeting nucleotide sequences complementary to the regulatory regionof a nucleotide sequence referenced herein or a substantially identicalsequence (e.g., promoter and/or enhancers) to form triple helicalstructures that prevent transcription of a gene in target cells (seee.g., Helene, Anticancer Drug Des. 6(6): 569-84 (1991); Helene et al.,Ann. N.Y. Acad. Sci. 660: 27-36 (1992); and Maher, Bioassays 14(12):807-15 (1992). Potential sequences that can be targeted for triple helixformation can be increased by creating a so-called “switchback” nucleicacid molecule. Switchback molecules are synthesized in an alternating5′-3′, 3′-5′ manner, such that they base pair with first one strand of aduplex and then the other, eliminating the necessity for a sizeablestretch of either purines or pyrimidines to be present on one strand ofa duplex.

Osteoporosis directed molecules include RNAi and siRNA nucleic acids.Gene expression may be inhibited by the introduction of double-strandedRNA (dsRNA), which induces potent and specific gene silencing, aphenomenon called RNA interference or RNAi. See, e.g., Fire et al., U.S.Pat. No. 6,506,559; Tuschl et al. PCT International Publication No. WO01/75164; Kay et al. PCT International Publication No. WO 03/010180A1;or Bosher J M, Labouesse, Nat Cell Biol 2000 February; 2(2):E31-6. Thisprocess has been improved by decreasing the size of the double-strandedRNA to 20-24 base pairs (to create small-interfering RNAs or siRNAs)that “switched off” genes in mammalian cells without initiating an acutephase response, i.e., a host defense mechanism that often results incell death (see, e.g., Caplen et al. Proc Natl Acad Sci USA. 2001 Aug.14; 98(17):9742-7 and Elbashir et al. Methods 2002 February; 26(2):199-213). There is increasing evidence of post-transcriptional genesilencing by RNA interference (RNAi) for inhibiting targeted expressionin mammalian cells at the mRNA level, in human cells. There isadditional evidence of effective methods for inhibiting theproliferation and migration of tumor cells in human patients, and forinhibiting metastatic cancer development (see, e.g., U.S. PatentApplication No. US2001000993183; Caplen et al. Proc Natl Acad Sci USA;and Abdeffahmani et al. Mol Cell Biol 2001 November 21(21):7256-67).

An “siRNA” or “RNAi” refers to a nucleic acid that forms a doublestranded RNA and has the ability to reduce or inhibit expression of agene or target gene when the siRNA is delivered to or expressed in thesame cell as the gene or target gene. “siRNA” refers to shortdouble-stranded RNA formed by the complementary strands. Complementaryportions of the siRNA that hybridize to form the double strandedmolecule often have substantial or complete identity to the targetmolecule sequence. In one embodiment, an siRNA refers to a nucleic acidthat has substantial or complete identity to a target gene and forms adouble stranded siRNA.

When designing the siRNA molecules, the targeted region often isselected from a given DNA sequence beginning 50 to 100 nucleotidesdownstream of the start codon. See, e.g., Elbashir et al., Methods26:199-213 (2002). Initially, 5′ or 3′ UTRs and regions nearby the startcodon were avoided assuming that UTR-binding proteins and/or translationinitiation complexes may interfere with binding of the siRNP or RISCendonuclease complex. Sometimes regions of the target 23 nucleotides inlength conforming to the sequence motif AA(N19)TT (N, an nucleotide),and regions with approximately 30% to 70% G/C-content (often about 50%G/C-content) often are selected. If no suitable sequences are found, thesearch often is extended using the motif NA(N21). The sequence of thesense siRNA sometimes corresponds to (N19) TT or N21 (position 3 to 23of the 23-nt motif), respectively. In the latter case, the 3′ end of thesense siRNA often is converted to TT. The rationale for this sequenceconversion is to generate a symmetric duplex with respect to thesequence composition of the sense and antisense 3′ overhangs. Theantisense siRNA is synthesized as the complement to position 1 to 21 ofthe 23-nt motif. Because position 1 of the 23-nt motif is not recognizedsequence-specifically by the antisense siRNA, the 3′-most nucleotideresidue of the antisense siRNA can be chosen deliberately. However, thepenultimate nucleotide of the antisense siRNA (complementary to position2 of the 23-nt motif) often is complementary to the targeted sequence.For simplifying chemical synthesis, TT often is utilized. siRNAscorresponding to the target motif NAR(N17)YNN, where R is purine (A,G)and Y is pyrimidine (C,U), often are selected. Respective 21 nucleotidesense and antisense siRNAs often begin with a purine nucleotide and canalso be expressed from pol III expression vectors without a change intargeting site. Expression of RNAs from pol III promoters often isefficient when the first transcribed nucleotide is a purine.

The sequence of the siRNA can correspond to the full length target gene,or a subsequence thereof. Often, the siRNA is about 15 to about 50nucleotides in length (e.g., each complementary sequence of the doublestranded siRNA is 15-50 nucleotides in length, and the double strandedsiRNA is about 15-50 base pairs in length, sometimes about 20-30nucleotides in length or about 20-25 nucleotides in length, e.g., 20,21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleotides in length. ThesiRNA sometimes is about 21 nucleotides in length. Methods of usingsiRNA are well known in the art, and specific siRNA molecules may bepurchased from a number of companies including Dharmacon Research, Inc.An siRNA molecule sometimes is of a different chemical composition ascompared to native RNA that imparts increased stability in cells (e.g.,decreased susceptibility to degradation), and sometimes includes one ormore modifications in siSTABLE RNA described at the http addresswww.dharmacon.com.

Antisense, ribozyme, RNAi and siRNA nucleic acids can be altered to formmodified nucleic acid molecules. The nucleic acids can be altered atbase moieties, sugar moieties or phosphate backbone moieties to improvestability, hybridization, or solubility of the molecule. For example,the deoxyribose phosphate backbone of nucleic acid molecules can bemodified to generate peptide nucleic acids (see Hyrup et al., Bioorganic& Medicinal Chemistry 4 (1): 5-23 (1996)). As used herein, the terms“peptide nucleic acid” or “PNA” refers to a nucleic acid mimic such as aDNA mimic, in which the deoxyribose phosphate backbone is replaced by apseudopeptide backbone and only the four natural nucleobases areretained. The neutral backbone of a PNA can allow for specifichybridization to DNA and RNA under conditions of low ionic strength.Synthesis of PNA oligomers can be performed using standard solid phasepeptide synthesis protocols as described, for example, in Hyrup et al.,(1996) supra and Perry-O'Keefe et al., Proc. Natl. Acad. Sci. 93:14670-675 (1996).

PNA nucleic acids can be used in prognostic, diagnostic, and therapeuticapplications. For example, PNAs can be used as antisense or antigeneagents for sequence-specific modulation of gene expression by, forexample, inducing transcription or translation arrest or inhibitingreplication. PNA nucleic acid molecules can also be used in the analysisof single base pair mutations in a gene, (e.g., by PNA-directed PCRclamping); as “artificial restriction enzymes” when used in combinationwith other enzymes, (e.g., S1 nucleases (Hyrup (1996) supra)); or asprobes or primers for DNA sequencing or hybridization (Hyrup et al.,(1996) supra; Perry-O'Keefe supra).

In other embodiments, oligonucleotides may include other appended groupssuch as peptides (e.g., for targeting host cell receptors in vivo), oragents facilitating transport across cell membranes (see e.g., Letsingeret al., Proc. Natl. Acad. Sci. USA 86: 6553-6556 (1989); Lemaitre etal., Proc. Natl. Acad. Sci. USA 84: 648-652 (1987); PCT Publication No.WO88/09810) or the blood-brain barrier (see, e.g., PCT Publication No.WO 89/10134). In addition, oligonucleotides can be modified withhybridization-triggered cleavage agents (See, e.g., Krol et al.,Bio-Techniques 6: 958-976 (1988)) or intercalating agents. (See, e.g.,Zon, Pharm. Res. 5: 539-549 (1988)). To this end, the oligonucleotidemay be conjugated to another molecule, (e.g., a peptide, hybridizationtriggered cross-linking agent, transport agent, orhybridization-triggered cleavage agent).

Also included herein are molecular beacon oligonucleotide primer andprobe molecules having one or more regions complementary to a CETP,PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleotide sequence or a substantiallyidentical sequence thereof, two complementary regions one having afluorophore and one a quencher such that the molecular beacon is usefulfor quantifying the presence of the nucleic acid in a sample. Molecularbeacon nucleic acids are described, for example, in Lizardi et al., U.S.Pat. No. 5,854,033; Nazarenko et al., U.S. Pat. No. 5,866,336, and Livaket al., U.S. Pat. No. 5,876,930.

Antibody

The term “antibody” as used herein refers to an immunoglobulin moleculeor immunologically active portion thereof, i.e., an antigen-bindingportion. Examples of immunologically active portions of immunoglobulinmolecules include F(ab) and F(ab′)₂ fragments which can be generated bytreating the antibody with an enzyme such as pepsin. An antibodysometimes is a polyclonal, monoclonal, recombinant (e.g., a chimeric orhumanized), fully human, non-human (e.g., murine), or a single chainantibody. An antibody may have effector function and can fix complement,and is sometimes coupled to a toxin or imaging agent.

Antibodies

A full-length polypeptide or antigenic peptide fragment encoded by anucleotide sequence referenced herein can be used as an immunogen or canbe used to identify antibodies made with other immunogens, e.g., cells,membrane preparations, and the like. An antigenic peptide often includesat least 8 amino acid residues of the amino acid sequences encoded by anucleotide sequence referenced herein, or substantially identicalsequence thereof, and encompasses an epitope. Antigenic peptidessometimes include 10 or more amino acids, 15 or more amino acids, 20 ormore amino acids, or 30 or more amino acids. Hydrophilic and hydrophobicfragments of polypeptides sometimes are used as immunogens.

Epitopes encompassed by the antigenic peptide are regions located on thesurface of the polypeptide (e.g., hydrophilic regions) as well asregions with high antigenicity. For example, an Emini surfaceprobability analysis of the human polypeptide sequence can be used toindicate the regions that have a particularly high probability of beinglocalized to the surface of the polypeptide and are thus likely toconstitute surface residues useful for targeting antibody production.The antibody may bind an epitope on any domain or region on polypeptidesdescribed herein.

Also, chimeric, humanized, and completely human antibodies are usefulfor applications which include repeated administration to subjects.Chimeric and humanized monoclonal antibodies, comprising both human andnon-human portions, can be made using standard recombinant DNAtechniques. Such chimeric and humanized monoclonal antibodies can beproduced by recombinant DNA techniques known in the art, for exampleusing methods described in Robinson et al International Application No.PCT/US86/02269; Akira, et al European Patent Application 184,187;Taniguchi, M., European Patent Application 171,496; Morrison et alEuropean Patent Application 173,494; Neuberger et al PCT InternationalPublication No. WO 86/01533; Cabilly et al U.S. Pat. No. 4,816,567;Cabilly et al European Patent Application 125,023; Better et al.,Science 240: 1041-1043 (1988); Liu et al., Proc. Natl. Acad. Sci. USA84: 3439-3443 (1987); Liu et al., J. Immunol. 139: 3521-3526 (1987); Sunet al., Proc. Natl. Acad. Sci. USA 84: 214-218 (1987); Nishimura et al.,Canc. Res. 47: 999-1005 (1987); Wood et al., Nature 314: 446-449 (1985);and Shaw et al., J. Natl. Cancer Inst. 80: 1553-1559 (1988); Morrison,S. L., Science 229:1202-1207(1985); Oi et al., BioTechniques 4:214(1986); Winter U.S. Pat. No. 5,225,539; Jones et al., Nature 321:552-525 (1986); Verhoeyan et al., Science 239: 1534; and Beidler et al.,J. Immunol. 141: 4053-4060 (1988).

Completely human antibodies are particularly desirable for therapeutictreatment of human patients. Such antibodies can be produced usingtransgenic mice that are incapable of expressing endogenousimmunoglobulin heavy and light chains genes, but which can express humanheavy and light chain genes. See, for example, Lonberg and Huszar, Int.Rev. Immunol. 13: 65-93 (1995); and U.S. Pat. Nos. 5,625,126; 5,633,425;5,569,825; 5,661,016; and 5,545,806. In addition, companies such asAbgenix, Inc. (Fremont, Calif.) and Medarex, Inc. (Princeton, N.J.), canbe engaged to provide human antibodies directed against a selectedantigen using technology similar to that described above. Completelyhuman antibodies that recognize a selected epitope also can be generatedusing a technique referred to as “guided selection.” In this approach aselected non-human monoclonal antibody (e.g., a murine antibody) is usedto guide the selection of a completely human antibody recognizing thesame epitope. This technology is described for example by Jespers etal., Bio/Technology 12: 899-903 (1994).

An antibody can be a single chain antibody. A single chain antibody(scFV) can be engineered (see, e.g., Colcher et al., Ann. N Y Acad. Sci.880: 263-80 (1999); and Reiter, Clin. Cancer Res. 2: 245-52 (1996)).Single chain antibodies can be dimerized or multimerized to generatemultivalent antibodies having specificities for different epitopes ofthe same target polypeptide.

Antibodies also may be selected or modified so that they exhibit reducedor no ability to bind an Fc receptor. For example, an antibody may be anisotype or subtype, fragment or other mutant, which does not supportbinding to an Fc receptor (e.g., it has a mutagenized or deleted Fcreceptor binding region).

Also, an antibody (or fragment thereof) may be conjugated to atherapeutic moiety such as a cytotoxin, a therapeutic agent or aradioactive metal ion. A cytotoxin or cytotoxic agent includes any agentthat is detrimental to cells. Examples include taxol, cytochalasin B,gramicidin D, ethidium bromide, emetine, mitomycin, etoposide,tenoposide, vincristine, vinblastine, colchicin, doxorubicin,daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin,actinomycin D, l dehydrotestosterone, glucocorticoids, procaine,tetracaine, lidocaine, propranolol, and puromycin and analogs orhomologs thereof. Therapeutic agents include, but are not limited to,antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine,cytarabine, 5-fluorouracil decarbazine), alkylating agents (e.g.,mechlorethamine, thiotepa chlorambucil, melphalan, carmustine (BCNU) andlomustine (CCNU), cyclophosphamide, busulfan, dibromomannitol,streptozotocin, mitomycin C, and cis-dichlorodiamine platinum (II) (DDP)cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) anddoxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin),bleomycin, mithramycin, and anthramycin (AMC)), and anti-mitotic agents(e.g., vincristine and vinblastine).

Antibody conjugates can be used for modifying a given biologicalresponse. For example, the drug moiety may be a protein or polypeptidepossessing a desired biological activity. Such proteins may include, forexample, a toxin such as abrin, ricin A, pseudomonas exotoxin, ordiphtheria toxin; a polypeptide such as tumor necrosis factor,γ-interferon, α-interferon, nerve growth factor, platelet derived growthfactor, tissue plasminogen activator; or, biological response modifierssuch as, for example, lymphokines, interleukin-1 (“IL-1”), interleukin-2(“IL-2”), interleukin-6 (“IL-6”), granulocyte macrophage colonystimulating factor (“GM-CSF”), granulocyte colony stimulating factor“G-CSF”), or other growth factors. Also, an antibody can be conjugatedto a second antibody to form an antibody heteroconjugate as described bySegal in U.S. Pat. No. 4,676,980, for example.

An antibody (e.g., monoclonal antibody) can be used to isolate targetpolypeptides by standard techniques, such as affinity chromatography orimmunoprecipitation. Moreover, an antibody can be used to detect atarget polypeptide (e.g., in a cellular lysate or cell supernatant) inorder to evaluate the abundance and pattern of expression of thepolypeptide. Antibodies can be used diagnostically to monitorpolypeptide levels in tissue as part of a clinical testing procedure,e.g., to determine the efficacy of a given treatment regimen. Detectioncan be facilitated by coupling (i.e., physically linking) the antibodyto a detectable substance (i.e., antibody labeling). Examples ofdetectable substances include various enzymes, prosthetic groups,fluorescent materials, luminescent materials, bioluminescent materials,and radioactive materials. Examples of suitable enzymes includehorseradish peroxidase, alkaline phosphatase, β-galactosidase, oracetylcholinesterase; examples of suitable prosthetic group complexesinclude streptavidin/biotin and avidin/biotin; examples of suitablefluorescent materials include umbelliferone, fluorescein, fluoresceinisothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansylchloride or phycoerythrin; an example of a luminescent material includesluminol; examples of bioluminescent materials include luciferase,luciferin, and aequorin, and examples of suitable radioactive materialinclude ¹²⁵I, ¹³¹I, ³⁵S or ³H. Also, an antibody can be utilized as atest molecule for determining whether it can treat osteoporosis, and asa therapeutic for administration to a subject for treating osteoporosis.

An antibody can be made by immunizing with a purified antigen, or afragment thereof, e.g., a fragment described herein, a membraneassociated antigen, tissues, e.g., crude tissue preparations, wholecells, preferably living cells, lysed cells, or cell fractions.

Included herein are antibodies which bind only a native polypeptide,only denatured or otherwise non-native polypeptide, or which bind both,as well as those having linear or conformational epitopes.Conformational epitopes sometimes can be identified by selectingantibodies that bind to native but not denatured polypeptide. Alsofeatured are antibodies that specifically bind to a polypeptide variantassociated with low BMD.

Methods for Identifying Candidate Therapeutics for Treating Osteoporosis

Current treatment for osteoporosis can help stop further bone loss andfractures, but there is still a clear need for specific anabolic agentsthat considerably increase bone formation in people who have alreadysuffered substantial bone loss. There are no such drugs currentlyapproved. Known osteoporosis treatments include bone-active phosphonates(See, e.g., U.S. Pat. No. 6,329,354), bisphosphonates, e.g., alendronate(Fosamax®) and risedronate (Actonel®); calcitonin (Niacalcin®); estrogenand hormone therapy, e.g., estrogens (Climara®, Estrace®, Estraderm®,Estratab®, Ogen®, Ortho-Est®, Vivelle®, Premarin®, and others) andestrogens and progestins (brand names, such as Activella™, FemHrt®,Premphase®, Prempro®, and others); parathyroid hormone, e.g.,teriparatide {PTH 1-34} (Forteo®); as well as, estrogen and estrogenreceptor modulators (SERMs), e.g., raloxifene HCl (Evista®), sodiumfluoride and vitamin D metabolites. Any of the above therapeutics may beadministered alone or in combination (e.g., bone-active phosphonates andestrogen and hormone therapy). Current therapeutic approaches werelargely developed in the absence of defined molecular targets or even asolid understanding of disease pathogenesis. Therefore, provided aremethods of identifying candidate therapeutics that target biochemicalpathways related to the development of osteoporosis.

Thus, featured herein are methods for identifying a candidatetherapeutic for treating osteoporosis. The methods comprise contacting atest molecule with a target molecule in a system. A “target molecule” asused herein refers to a CETP, PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleicacid, a substantially identical nucleic acid thereof, or a fragmentthereof, and an encoded polypeptide of the foregoing. The methods alsocomprise determining the presence or absence of an interaction betweenthe test molecule and the target molecule, where the presence of aninteraction between the test molecule and the nucleic acid orpolypeptide identifies the test molecule as a candidate osteoporosistherapeutic. The interaction between the test molecule and the targetmolecule may be quantified.

Test molecules and candidate therapeutics include, but are not limitedto, compounds, antisense nucleic acids, siRNA molecules, ribozymes,polypeptides or proteins encoded by a CETP, PROL4, GRID2, PDE4D orGPX3/TNIP1 nucleotide sequence, or a substantially identical sequence orfragment thereof, and immunotherapeutics (e.g., antibodies andHLA-presented polypeptide fragments). A test molecule or candidatetherapeutic may act as a modulator of target molecule concentration ortarget molecule function in a system. A “modulator” may agonize (i.e.,up-regulates) or antagonize (i.e., down-regulates) a target moleculeconcentration partially or completely in a system by affecting suchcellular functions as DNA replication and/or DNA processing (e.g. DNAmethylation or DNA repair), RNA transcription and/or RNA processing(e.g., removal of intronic sequences and/or translocation of splicedmRNA from the nucleus), polypeptide production (e.g., translation of thepolypeptide from mRNA), and/or polypeptide post-translationalmodification (e.g., glycosylation, phosphorylation, and proteolysis ofpro-polypeptides). A modulator may also agonize or antagonize abiological function of a target molecule partially or completely, wherethe function may include adopting a certain structural conformation,interacting with one or more binding partners, ligand binding, catalysis(e.g., phosphorylation, dephosphorylation, hydrolysis, methylation, andisomerization), and an effect upon a cellular event (e.g., effectingprogression of osteoporosis). In certain embodiments, a candidatetherapeutic increases BMD.

As used herein, the term “system” refers to a cell free in vitroenvironment and a cell-based environment such as a collection of cells,a tissue, an organ, or an organism. A system is “contacted” with a testmolecule in a variety of manners, including adding molecules in solutionand allowing them to interact with one another by diffusion, cellinjection, and any administration routes in an animal. As used herein,the term “interaction” refers to an effect of a test molecule on testmolecule, where the effect sometimes is binding between the testmolecule and the target molecule, and sometimes is an observable changein cells, tissue, or organism.

There are many standard methods for detecting the presence or absence ofinteraction between a test molecule and a target molecule. For example,titrametric, acidimetric, radiometric, NMR, monolayer, polarographic,spectrophotometric, fluorescent, and ESR assays probative of a targetmolecule interaction may be utilized.

Test molecule/target molecule interactions can be detected and/orquantified using assays known in the art. For example, an interactioncan be determined by labeling the test molecule and/or the targetmolecule, where the label is covalently or non-covalently attached tothe test molecule or target molecule. The label is sometimes aradioactive molecule such as ¹²⁵I, ¹³¹I, ³⁵S or ³H, which can bedetected by direct counting of radioemission or by scintillationcounting. Also, enzymatic labels such as horseradish peroxidase,alkaline phosphatase, or luciferase may be utilized where the enzymaticlabel can be detected by determining conversion of an appropriatesubstrate to product. In addition, presence or absence of an interactioncan be determined without labeling. For example, a microphysiometer(e.g., Cytosensor) is an analytical instrument that measures the rate atwhich a cell acidifies its environment using a light-addressablepotentiometric sensor (LAPS). Changes in this acidification rate can beused as an indication of an interaction between a test molecule andtarget molecule (McConnell, H. M. et al., Science 257: 1906-1912(1992)).

In cell-based systems, cells typically include a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleic acid, an encoded polypeptide, orsubstantially identical nucleic acid or polypeptide thereof, and areoften of mammalian origin, although the cell can be of any origin. Wholecells, cell homogenates, and cell fractions (e.g., cell membranefractions) can be subjected to analysis. Where interactions between atest molecule with a target polypeptide are monitored, soluble and/ormembrane bound forms of the polypeptide may be utilized. Wheremembrane-bound forms of the polypeptide are used, it may be desirable toutilize a solubilizing agent. Examples of such solubilizing agentsinclude non-ionic detergents such as n-octylglucoside,n-dodecylglucoside, n-dodecylmaltoside, octanoyl-N-methylglucamide,decanoyl-N-methylglucamide, Triton® X-100, Triton® X-114, Thesit®,Isotridecypoly(ethylene glycol ether)_(n),3-[(3-cholamidopropyl)dimethylamminio]-1-propane sulfonate (CHAPS),3-[(3-cholamidopropyl)dimethylamminio]-2-hydroxy-1-propane sulfonate(CHAPSO), or N-dodecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate.

An interaction between a test molecule and target molecule also can bedetected by monitoring fluorescence energy transfer (FET) (see, e.g.,Lakowicz et al., U.S. Pat. No. 5,631,169; Stavrianopoulos et al. U.S.Pat. No. 4,868,103). A fluorophore label on a first, “donor” molecule isselected such that its emitted fluorescent energy will be absorbed by afluorescent label on a second, “acceptor” molecule, which in turn isable to fluoresce due to the absorbed energy. Alternately, the “donor”polypeptide molecule may simply utilize the natural fluorescent energyof tryptophan residues. Labels are chosen that emit differentwavelengths of light, such that the “acceptor” molecule label may bedifferentiated from that of the “donor”. Since the efficiency of energytransfer between the labels is related to the distance separating themolecules, the spatial relationship between the molecules can beassessed. In a situation in which binding occurs between the molecules,the fluorescent emission of the “acceptor” molecule label in the assayshould be maximal. An FET binding event can be conveniently measuredthrough standard fluorometric detection means well known in the art(e.g. using a fluorimeter).

In another embodiment, determining the presence or absence of aninteraction between a test molecule and a target molecule can beeffected by monitoring surface plasmon resonance (see, e.g., Sjolander &Urbaniczk, Anal. Chem. 63: 2338-2345 (1991) and Szabo et al., Curr.Opin. Struct. Biol. 5: 699-705 (1995)). “Surface plasmon resonance” or“biomolecular interaction analysis (BIA)” can be utilized to detectbiospecific interactions in real time, without labeling any of theinteractants (e.g., BIAcore). Changes in the mass at the binding surface(indicative of a binding event) result in alterations of the refractiveindex of light near the surface (the optical phenomenon of surfaceplasmon resonance (SPR)), resulting in a detectable signal which can beused as an indication of real-time reactions between biologicalmolecules.

In another embodiment, the target molecule or test molecules areanchored to a solid phase, facilitating the detection of targetmolecule/test molecule complexes and separation of the complexes fromfree, uncomplexed molecules. The target molecule or test molecule isimmobilized to the solid support. In an embodiment, the target moleculeis anchored to a solid surface, and the test molecule, which is notanchored, can be labeled, either directly or indirectly, with detectablelabels discussed herein.

It may be desirable to immobilize a target molecule, an anti-targetmolecule antibody, and/or test molecules to facilitate separation oftarget molecule/test molecule complexes from uncomplexed forms, as wellas to accommodate automation of the assay. The attachment between a testmolecule and/or target molecule and the solid support may be covalent ornon-covalent (see, e.g., U.S. Pat. No. 6,022,688 for non-covalentattachments). The solid support may be one or more surfaces of thesystem, such as one or more surfaces in each well of a microtiter plate,a surface of a silicon wafer, a surface of a bead (see, e.g., Lam,Nature 354: 82-84 (1991)) that is optionally linked to another solidsupport, or a channel in a microfluidic device, for example. Types ofsolid supports, linker molecules for covalent and non-covalentattachments to solid supports, and methods for immobilizing nucleicacids and other molecules to solid supports are well known (see, e.g.,U.S. Pat. Nos. 6,261,776; 5,900,481; 6,133,436; and 6,022,688; and WIPOpublication WO 01/18234).

In an embodiment, target molecule may be immobilized to surfaces viabiotin and streptavidin. For example, biotinylated target polypeptidecan be prepared from biotin-NHS (N-hydroxy-succinimide) using techniquesknown in the art (e.g., biotinylation kit, Pierce Chemicals, Rockford,Ill.), and immobilized in the wells of streptavidin-coated 96 wellplates (Pierce Chemical). In another embodiment, a target polypeptidecan be prepared as a fusion polypeptide. For example,glutathione-S-transferase/target polypeptide fusion can be adsorbed ontoglutathione sepharose beads (Sigma Chemical, St. Louis, Mo.) orglutathione derivitized microtiter plates, which are then combined witha test molecule under conditions conducive to complex formation (e.g.,at physiological conditions for salt and pH). Following incubation, thebeads or microtiter plate wells are washed to remove any unboundcomponents, or the matrix is immobilized in the case of beads, andcomplex formation is determined directly or indirectly as describedabove. Alternatively, the complexes can be dissociated from the matrix,and the level of target molecule binding or activity is determined usingstandard techniques.

In an embodiment, the non-immobilized component is added to the coatedsurface containing the anchored component. After the reaction iscomplete, unreacted components are removed (e.g., by washing) underconditions such that a significant percentage of complexes formed willremain immobilized to the solid surface. The detection of complexesanchored on the solid surface can be accomplished in a number ofmanners. Where the previously non-immobilized component is pre-labeled,the detection of label immobilized on the surface indicates thatcomplexes were formed. Where the previously non-immobilized component isnot pre-labeled, an indirect label can be used to detect complexesanchored on the surface, e.g., by adding a labeled antibody specific forthe immobilized component, where the antibody, in turn, can be directlylabeled or indirectly labeled with, e.g., a labeled anti-Ig antibody.

In another embodiment, an assay is performed utilizing antibodies thatspecifically bind target molecule or test molecule but do not interferewith binding of the target molecule to the test molecule. Suchantibodies can be derivitized to a solid support, and unbound targetmolecule may be immobilized by antibody conjugation. Methods fordetecting such complexes, in addition to those described above for theGST-immobilized complexes, include immunodetection of complexes usingantibodies reactive with the target molecule, as well as enzyme-linkedassays which rely on detecting an enzymatic activity associated with thetarget molecule.

Cell free assays also can be conducted in a liquid phase. In such anassay, reaction products are separated from unreacted components, by anyof a number of standard techniques, including but not limited to:differential centrifugation (see, e.g., Rivas, G., and Minton, TrendsBiochem Sci August; 18(8): 284-7 (1993)); chromatography (gel filtrationchromatography, ion-exchange chromatography); electrophoresis (see,e.g., Ausubel et al., eds. Current Protocols in Molecular Biology, J.Wiley: New York (1999)); and immunoprecipitation (see, e.g., Ausubel etal., eds., supra). Media and chromatographic techniques are known to oneskilled in the art (see, e.g., Heegaard, J Mol. Recognit. Winter;11(1-6): 141-8 (1998); Hage & Tweed, J. Chromatogr. B Biomed. Sci. Appl.October 10; 699 (1-2): 499-525 (1997)). Further, fluorescence energytransfer may also be conveniently utilized, as described herein, todetect binding without further purification of the complex fromsolution.

In another embodiment, modulators of target molecule expression areidentified. For example, a cell or cell free mixture is contacted with acandidate compound and the expression of target mRNA or targetpolypeptide is evaluated relative to the level of expression of targetmRNA or target polypeptide in the absence of the candidate compound.When expression of target mRNA or target polypeptide is greater in thepresence of the candidate compound than in its absence, the candidatecompound is identified as an agonist of target mRNA or targetpolypeptide expression. Alternatively, when expression of target mRNA ortarget polypeptide is less (e.g., less with statistical significance) inthe presence of the candidate compound than in its absence, thecandidate compound is identified as an antagonist or inhibitor of targetmRNA or target polypeptide expression. The level of target mRNA ortarget polypeptide expression can be determined by methods describedherein.

In another embodiment, binding partners that interact with a targetmolecule are detected. The target molecules can interact with one ormore cellular or extracellular macromolecules, such as polypeptides invivo, and these interacting molecules are referred to herein as “bindingpartners.” Binding partners can agonize or antagonize target moleculebiological activity. Also, test molecules that agonize or antagonizeinteractions between target molecules and binding partners can be usefulas therapeutic molecules as they can up-regulate or down-regulatedtarget molecule activity in vivo and thereby treat osteoporosis.

Binding partners of target molecules can be identified by methods knownin the art. For example, binding partners may be identified by lysingcells and analyzing cell lysates by electrophoretic techniques.Alternatively, a two-hybrid assay or three-hybrid assay can be utilized(see, e.g., U.S. Pat. No. 5,283,317; Zervos et al., Cell 72:223-232(1993); Madura et al., J. Biol. Chem. 268: 12046-12054 (1993); Bartel etal., Biotechniques 14: 920-924 (1993); Iwabuchi et al., Oncogene 8:1693-1696 (1993); and Brent WO94/10300). A two-hybrid system is based onthe modular nature of most transcription factors, which consist ofseparable DNA-binding and activation domains. The assay often utilizestwo different DNA constructs. In one construct, a CETP, PROL4, GRID2,PDE4D or GPX3/TNIP1 nucleic acid (sometimes referred to as the “bait”)is fused to a gene encoding the DNA binding domain of a knowntranscription factor (e.g., GAL-4). In another construct, a DNA sequencefrom a library of DNA sequences that encodes a potential binding partner(sometimes referred to as the “prey”) is fused to a gene that encodes anactivation domain of the known transcription factor. Sometimes, a CETP,PROL4, GRID2, PDE4D or GPX3/TNIP1 nucleic acid can be fused to theactivation domain. If the “bait” and the “prey” molecules interact invivo, the DNA-binding and activation domains of the transcription factorare brought into close proximity. This proximity allows transcription ofa reporter gene (e.g., LacZ) which is operably linked to atranscriptional regulatory site responsive to the transcription factor.Expression of the reporter gene can be detected and cell coloniescontaining the functional transcription factor can be isolated and usedto identify the potential binding partner.

In an embodiment for identifying test molecules that antagonize oragonize complex formation between target molecules and binding partners,a reaction mixture containing the target molecule and the bindingpartner is prepared, under conditions and for a time sufficient to allowcomplex formation. The reaction mixture often is provided in thepresence or absence of the test molecule. The test molecule can beincluded initially in the reaction mixture, or can be added at a timesubsequent to the addition of the target molecule and its bindingpartner. Control reaction mixtures are incubated without the testmolecule or with a placebo. Formation of any complexes between thetarget molecule and the binding partner then is detected. Decreasedformation of a complex in the reaction mixture containing test moleculeas compared to in a control reaction mixture indicates that the moleculeantagonizes target molecule/binding partner complex formation.Alternatively, increased formation of a complex in the reaction mixturecontaining test molecule as compared to in a control reaction mixtureindicates that the molecule agonizes target molecule/binding partnercomplex formation. In another embodiment, complex formation of targetmolecule/binding partner can be compared to complex formation of mutanttarget molecule/binding partner (e.g., amino acid modifications in atarget polypeptide). Such a comparison can be important in those caseswhere it is desirable to identify test molecules that modulateinteractions of mutant but not non-mutated target gene products.

The assays can be conducted in a heterogeneous or homogeneous format. Inheterogeneous assays, target molecule and/or the binding partner areimmobilized to a solid phase, and complexes are detected on the solidphase at the end of the reaction. In homogeneous assays, the entirereaction is carried out in a liquid phase. In either approach, the orderof addition of reactants can be varied to obtain different informationabout the molecules being tested. For example, test compounds thatagonize target molecule/binding partner interactions can be identifiedby conducting the reaction in the presence of the test molecule in acompetition format. Alternatively, test molecules that agonize preformedcomplexes, e.g., molecules with higher binding constants that displaceone of the components from the complex, can be tested by adding the testcompound to the reaction mixture after complexes have been formed.

In a heterogeneous assay embodiment, the target molecule or the bindingpartner is anchored onto a solid surface (e.g., a microtiter plate),while the non-anchored species is labeled, either directly orindirectly. The anchored molecule can be immobilized by non-covalent orcovalent attachments. Alternatively, an immobilized antibody specificfor the molecule to be anchored can be used to anchor the molecule tothe solid surface. The partner of the immobilized species is exposed tothe coated surface with or without the test molecule. After the reactionis complete, unreacted components are removed (e.g., by washing) suchthat a significant portion of any complexes formed will remainimmobilized on the solid surface. Where the non-immobilized species ispre-labeled, the detection of label immobilized on the surface isindicative of complex. Where the non-immobilized species is notpre-labeled, an indirect label can be used to detect complexes anchoredto the surface; e.g., by using a labeled antibody specific for theinitially non-immobilized, species. Depending upon the order of additionof reaction components, test compounds that inhibit complex formation orthat disrupt preformed complexes can be detected.

In another embodiment, the reaction can be conducted in a liquid phasein the presence or absence of test molecule, where the reaction productsare separated from unreacted components, and the complexes are detected(e.g., using an immobilized antibody specific for one of the bindingcomponents to anchor any complexes formed in solution, and a labeledantibody specific for the other partner to detect anchored complexes).Again, depending upon the order of addition of reactants to the liquidphase, test compounds that inhibit complex or that disrupt preformedcomplexes can be identified.

In an alternate embodiment, a homogeneous assay can be utilized. Forexample, a preformed complex of the target gene product and theinteractive cellular or extracellular binding partner product isprepared. One or both of the target molecule or binding partner islabeled, and the signal generated by the label(s) is quenched uponcomplex formation (e.g., U.S. Pat. No. 4,109,496 that utilizes thisapproach for immunoassays). Addition of a test molecule that competeswith and displaces one of the species from the preformed complex willresult in the generation of a signal above background. In this way, testsubstances that disrupt target molecule/binding partner complexes can beidentified.

Candidate therapeutics for treating osteoporosis are identified from agroup of test molecules that interact with a target molecule. Testmolecules are normally ranked according to the degree with which theymodulate (e.g. agonize or antagonize) a function associated with thetarget molecule (e.g., DNA replication and/or processing, RNAtranscription and/or processing, polypeptide production and/orprocessing, and/or biological function/activity), and then top rankingmodulators are selected. Also, pharmacogenomic information describedherein can determine the rank of a modulator. The top 10% of ranked testmolecules often are selected for further testing as candidatetherapeutics, and sometimes the top 15%, 20%, or 25% of ranked testmolecules are selected for further testing as candidate therapeutics.Candidate therapeutics typically are formulated for administration to asubject.

Therapeutic Formulations

Formulations and pharmaceutical compositions typically include incombination with a pharmaceutically acceptable carrier one or moretarget molecule modulators. The modulator often is a test moleculeidentified as having an interaction with a target molecule by ascreening method described above. The modulator may be a compound, anantisense nucleic acid, a ribozyme, an antibody, or a binding partner.Also, formulations may comprise a target polypeptide or fragment thereofin combination with a pharmaceutically acceptable carrier.

As used herein, the term “pharmaceutically acceptable carrier” includessolvents, dispersion media, coatings, antibacterial and antifungalagents, isotonic and absorption delaying agents, and the like,compatible with pharmaceutical administration. Supplementary activecompounds can also be incorporated into the compositions. Pharmaceuticalcompositions can be included in a container, pack, or dispenser togetherwith instructions for administration.

A pharmaceutical composition typically is formulated to be compatiblewith its intended route of administration. Examples of routes ofadministration include parenteral, e.g., intravenous, intradermal,subcutaneous, oral (e.g., inhalation), transdermal (topical),transmucosal, and rectal administration. Solutions or suspensions usedfor parenteral, intradermal, or subcutaneous application can include thefollowing components: a sterile diluent such as water for injection,saline solution, fixed oils, polyethylene glycols, glycerin, propyleneglycol or other synthetic solvents; antibacterial agents such as benzylalcohol or methyl parabens; antioxidants such as ascorbic acid or sodiumbisulfite; chelating agents such as ethylenediaminetetraacetic acid;buffers such as acetates, citrates or phosphates and agents for theadjustment of tonicity such as sodium chloride or dextrose. pH can beadjusted with acids or bases, such as hydrochloric acid or sodiumhydroxide. The parenteral preparation can be enclosed in ampoules,disposable syringes or multiple dose vials made of glass or plastic.

Oral compositions generally include an inert diluent or an ediblecarrier. For the purpose of oral therapeutic administration, the activecompound can be incorporated with excipients and used in the form oftablets, troches, or capsules, e.g., gelatin capsules. Oral compositionscan also be prepared using a fluid carrier for use as a mouthwash.Pharmaceutically compatible binding agents, and/or adjuvant materialscan be included as part of the composition. The tablets, pills,capsules, troches and the like can contain any of the followingingredients, or compounds of a similar nature: a binder such asmicrocrystalline cellulose, gum tragacanth or gelatin; an excipient suchas starch or lactose, a disintegrating agent such as alginic acid,Primogel, or corn starch; a lubricant such as magnesium stearate orSterotes; a glidant such as colloidal silicon dioxide; a sweeteningagent such as sucrose or saccharin; or a flavoring agent such aspeppermint, methyl salicylate, or orange flavoring.

Pharmaceutical compositions suitable for injectable use include sterileaqueous solutions (where water soluble) or dispersions and sterilepowders for the extemporaneous preparation of sterile injectablesolutions or dispersion. For intravenous administration, suitablecarriers include physiological saline, bacteriostatic water, CremophorEL™ (BASF, Parsippany, N.J.) or phosphate buffered saline (PBS). In allcases, the composition must be sterile and should be fluid to the extentthat easy syringability exists. It should be stable under the conditionsof manufacture and storage and must be preserved against thecontaminating action of microorganisms such as bacteria and fungi. Thecarrier can be a solvent or dispersion medium containing, for example,water, ethanol, polyol (for example, glycerol, propylene glycol, andliquid polyethylene glycol, and the like), and suitable mixturesthereof. The proper fluidity can be maintained, for example, by the useof a coating such as lecithin, by the maintenance of the requiredparticle size in the case of dispersion and by the use of surfactants.Prevention of the action of microorganisms can be achieved by variousantibacterial and antifungal agents, for example, parabens,chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In manycases, it will be preferable to include isotonic agents, for example,sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in thecomposition. Prolonged absorption of the injectable compositions can bebrought about by including in the composition an agent which delaysabsorption, for example, aluminum monostearate and gelatin.

Sterile injectable solutions can be prepared by incorporating the activecompound in the required amount in an appropriate solvent with one or acombination of ingredients enumerated above, as required, followed byfiltered sterilization. Generally, dispersions are prepared byincorporating the active compound into a sterile vehicle which containsa basic dispersion medium and the required other ingredients from thoseenumerated above. In the case of sterile powders for the preparation ofsterile injectable solutions, the preferred methods of preparation arevacuum drying and freeze-drying which yields a powder of the activeingredient plus any additional desired ingredient from a previouslysterile-filtered solution thereof.

For administration by inhalation, the compounds are delivered in theform of an aerosol spray from pressured container or dispenser whichcontains a suitable propellant, e.g., a gas such as carbon dioxide, or anebulizer.

Systemic administration can also be by transmucosal or transdermalmeans. For transmucosal or transdermal administration, penetrantsappropriate to the barrier to be permeated are used in the formulation.Such penetrants are generally known in the art, and include, forexample, for transmucosal administration, detergents, bile salts, andfusidic acid derivatives. Transmucosal administration can beaccomplished through the use of nasal sprays or suppositories. Fortransdermal administration, the active compounds are formulated intoointments, salves, gels, or creams as generally known in the art.Molecules can also be prepared in the form of suppositories (e.g., withconventional suppository bases such as cocoa butter and otherglycerides) or retention enemas for rectal delivery.

In one embodiment, active molecules are prepared with carriers that willprotect the compound against rapid elimination from the body, such as acontrolled release formulation, including implants and microencapsulateddelivery systems. Biodegradable, biocompatible polymers can be used,such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid,collagen, polyorthoesters, and polylactic acid. Methods for preparationof such formulations will be apparent to those skilled in the art.Materials can also be obtained commercially from Alza Corporation andNova Pharmaceuticals, Inc. Liposomal suspensions (including liposomestargeted to infected cells with monoclonal antibodies to viral antigens)can also be used as pharmaceutically acceptable carriers. These can beprepared according to methods known to those skilled in the art, forexample, as described in U.S. Pat. No. 4,522,811.

It is advantageous to formulate oral or parenteral compositions indosage unit form for ease of administration and uniformity of dosage.Dosage unit form as used herein refers to physically discrete unitssuited as unitary dosages for the subject to be treated; each unitcontaining a predetermined quantity of active compound calculated toproduce the desired therapeutic effect in association with the requiredpharmaceutical carrier.

Toxicity and therapeutic efficacy of such compounds can be determined bystandard pharmaceutical procedures in cell cultures or experimentalanimals, e.g., for determining the LD₅₀ (the dose lethal to 50% of thepopulation) and the ED₅₀ (the dose therapeutically effective in 50% ofthe population). The dose ratio between toxic and therapeutic effects isthe therapeutic index and it can be expressed as the ratio LD₅₀/ED₅₀.Molecules which exhibit high therapeutic indices are preferred. Whilemolecules that exhibit toxic side effects may be used, care should betaken to design a delivery system that targets such compounds to thesite of affected tissue in order to minimize potential damage touninfected cells and, thereby, reduce side effects.

The data obtained from the cell culture assays and animal studies can beused in formulating a range of dosage for use in humans. The dosage ofsuch molecules lies preferably within a range of circulatingconcentrations that include the ED₅₀ with little or no toxicity. Thedosage may vary within this range depending upon the dosage formemployed and the route of administration utilized. For any moleculesused in the methods described herein, the therapeutically effective dosecan be estimated initially from cell culture assays. A dose may beformulated in animal models to achieve a circulating plasmaconcentration range that includes the IC₅₀ (i.e., the concentration ofthe test compound which achieves a half-maximal inhibition of symptoms)as determined in cell culture. Such information can be used to moreaccurately determine useful doses in humans. Levels in plasma may bemeasured, for example, by high performance liquid chromatography.

As defined herein, a therapeutically effective amount of protein orpolypeptide (i.e., an effective dosage) ranges from about 0.001 to 30mg/kg body weight, sometimes about 0.01 to 25 mg/kg body weight, oftenabout 0.1 to 20 mg/kg body weight, and more often about 1 to 10 mg/kg, 2to 9 mg/kg, 3 to 8 mg/kg, 4 to 7 mg/kg, or 5 to 6 mg/kg body weight. Theprotein or polypeptide can be administered one time per week for betweenabout 1 to 10 weeks, sometimes between 2 to 8 weeks, often between about3 to 7 weeks, and more often for about 4, 5, or 6 weeks. The skilledartisan will appreciate that certain factors may influence the dosageand timing required to effectively treat a subject, including but notlimited to the severity of the disease or disorder, previous treatments,the general health and/or age of the subject, and other diseasespresent. Moreover, treatment of a subject with a therapeuticallyeffective amount of a protein, polypeptide, or antibody can include asingle treatment or, preferably, can include a series of treatments.

With regard to polypeptide formulations, featured herein is a method fortreating osteoporosis in a subject, which comprises contacting one ormore cells in the subject with a first polypeptide, where the subjectcomprises a second polypeptide having one or more polymorphic variationsassociated with low BMD, and where the first polypeptide comprises fewerpolymorphic variations associated with low BMD than the secondpolypeptide. The first and second polypeptides are encoded by a nucleicacid which comprises a nucleotide sequence in SEQ ID NO's:1-10; anucleotide sequence which encodes a polypeptide consisting of an aminoacid sequence encoded by a nucleotide sequence referenced in SEQ IDNO's:1-10; a nucleotide sequence which encodes a polypeptide that is 90%or more identical to an amino acid sequence encoded by a nucleotidesequence of SEQ ID NO's:1-10 and a nucleotide sequence 90% or moreidentical to a nucleotide sequence in SEQ ID NO's:1-10. The subjectoften is a human.

For antibodies, a dosage of 0.1 mg/kg of body weight (generally 10 mg/kgto 20 mg/kg) is often utilized. If the antibody is to act in the brain,a dosage of 50 mg/kg to 100 mg/kg is often appropriate. Generally,partially human antibodies and fully human antibodies have a longerhalf-life within the human body than other antibodies. Accordingly,lower dosages and less frequent administration is often possible.Modifications such as lipidation can be used to stabilize antibodies andto enhance uptake and tissue penetration (e.g., into the brain). Amethod for lipidation of antibodies is described by Cruikshank et al.,J. Acquired Immune Deficiency Syndromes and Human Retrovirology 14:193(1997).

Antibody conjugates can be used for modifying a given biologicalresponse, the drug moiety is not to be construed as limited to classicalchemical therapeutic agents. For example, the drug moiety may be aprotein or polypeptide possessing a desired biological activity. Suchproteins may include, for example, a toxin such as abrin, ricin A,pseudomonas exotoxin, or diphtheria toxin; a polypeptide such as tumornecrosis factor, .alpha.-interferon, .beta.-interferon, nerve growthfactor, platelet derived growth factor, tissue plasminogen activator;or, biological response modifiers such as, for example, lymphokines,interleukin-1 (“IL-1”), interleukin-2 (“IL-2”), interleukin-6 (“IL-6”),granulocyte macrophage colony stimulating factor (“GM-CSF”), granulocytecolony stimulating factor (“G-CSF”), or other growth factors.Alternatively, an antibody can be conjugated to a second antibody toform an antibody heteroconjugate as described by Segal in U.S. Pat. No.4,676,980.

For compounds, exemplary doses include milligram or microgram amounts ofthe compound per kilogram of subject or sample weight, for example,about 1 microgram per kilogram to about 500 milligrams per kilogram,about 100 micrograms per kilogram to about 5 milligrams per kilogram, orabout 1 microgram per kilogram to about 50 micrograms per kilogram. Itis understood that appropriate doses of a small molecule depend upon thepotency of the small molecule with respect to the expression or activityto be modulated. When one or more of these small molecules is to beadministered to an animal (e.g., a human) in order to modulateexpression or activity of a polypeptide or nucleic acid describedherein, a physician, veterinarian, or researcher may, for example,prescribe a relatively low dose at first, subsequently increasing thedose until an appropriate response is obtained. In addition, it isunderstood that the specific dose level for any particular animalsubject will depend upon a variety of factors including the activity ofthe specific compound employed, the age, body weight, general health,gender, and diet of the subject, the time of administration, the routeof administration, the rate of excretion, any drug combination, and thedegree of expression or activity to be modulated.

With regard to nucleic acid formulations, gene therapy vectors can bedelivered to a subject by, for example, intravenous injection, localadministration (see, e.g., U.S. Pat. No. 5,328,470) or by stereotacticinjection (see e.g., Chen et al., (1994) Proc. Natl. Acad. Sci. USA91:3054-3057). Pharmaceutical preparations of gene therapy vectors caninclude a gene therapy vector in an acceptable diluent, or can comprisea slow release matrix in which the gene delivery vehicle is imbedded.Alternatively, where the complete gene delivery vector can be producedintact from recombinant cells (e.g., retroviral vectors) thepharmaceutical preparation can include one or more cells which producethe gene delivery system. Examples of gene delivery vectors aredescribed herein.

Therapeutic Methods

A therapeutic formulation described above can be administered to asubject in need of a therapeutic for inducing a desired biologicalresponse. Therapeutic formulations can be administered by any of thepaths described herein. With regard to both prophylactic and therapeuticmethods of treatment, such treatments may be specifically tailored ormodified, based on knowledge obtained from pharmacogenomic analysesdescribed herein.

As used herein, the term “treatment” is defined as the application oradministration of a therapeutic formulation to a subject, or applicationor administration of a therapeutic agent to an isolated tissue or cellline from a subject with the purpose to cure, heal, alleviate, relieve,alter, remedy, ameliorate, improve or affect osteoporosis, symptoms ofosteoporosis or a predisposition towards osteoporosis. A therapeuticformulation includes, but is not limited to, small molecules, peptides,antibodies, ribozymes and antisense oligonucleotides. Administration ofa therapeutic formulation can occur prior to the manifestation ofsymptoms characteristic of low BMD, such that osteoporosis is preventedor delayed in its progression. The appropriate therapeutic compositioncan be determined based on screening assays described herein.

As discussed, successful treatment of osteoporosis can be brought aboutby techniques that serve to agonize target molecule expression orfunction, or alternatively, antagonize target molecule expression orfunction. These techniques include administration of modulators thatinclude, but are not limited to, small organic or inorganic molecules;antibodies (including, for example, polyclonal, monoclonal, humanized,anti-idiotypic, chimeric or single chain antibodies, and Fab, F(ab′)₂and Fab expression library fragments, scFV molecules, andepitope-binding fragments thereof); and peptides, phosphopeptides, orpolypeptides.

Further, antisense and ribozyme molecules that inhibit expression of thetarget gene can also be used to reduce the level of target geneexpression, thus effectively reducing the level of target gene activity.Still further, triple helix molecules can be utilized in reducing thelevel of target gene activity. Antisense, ribozyme and triple helixmolecules are discussed above. It is possible that the use of antisense,ribozyme, and/or triple helix molecules to reduce or inhibit mutant geneexpression can also reduce or inhibit the transcription (triple helix)and/or translation (antisense, ribozyme) of mRNA produced by normaltarget gene alleles, such that the concentration of normal target geneproduct present can be lower than is necessary for a normal phenotype.In such cases, nucleic acid molecules that encode and express targetgene polypeptides exhibiting normal target gene activity can beintroduced into cells via gene therapy method. Alternatively, ininstances in that the target gene encodes an extracellular polypeptide,it can be preferable to co-administer normal target gene polypeptideinto the cell or tissue in order to maintain the requisite level ofcellular or tissue target gene activity.

Another method by which nucleic acid molecules may be utilized intreating or preventing osteoporosis is use of aptamer molecules specificfor target molecules. Aptamers are nucleic acid molecules having atertiary structure which permits them to specifically bind to ligands(see, e.g., Osborne, et al., Curr. Opin. Chem. Biol. 1(1): 5-9 (1997);and Patel, D. J., Curr. Opin. Chem. Biol. June; 1(1): 32-46 (1997)).

Yet another method of utilizing nucleic acid molecules for osteoporosistreatment is gene therapy, which can also be referred to as alleletherapy. Provided herein is a gene therapy method for treatingosteoporosis in a subject, which comprises contacting one or more cellsin the subject or from the subject with a nucleic acid having a firstnucleotide sequence. Genomic DNA in the subject comprises a secondnucleotide sequence having one or more polymorphic variations associatedwith low BMD (e.g., the second nucleic acid has a nucleotide sequence inSEQ ID NO's:1-10). The first and second nucleotide sequences typicallyare substantially identical to one another, and the first nucleotidesequence comprises fewer polymorphic variations associated with low BMDthan the second nucleotide sequence. The first nucleotide sequence maycomprise a gene sequence that encodes a full-length polypeptide or afragment thereof. The subject is often a human. Allele therapy methodsoften are utilized in conjunction with a method of first determiningwhether a subject has genomic DNA that includes polymorphic variantsassociated with low BMD.

In another allele therapy embodiment, provided herein is a method whichcomprises contacting one or more cells in the subject or from thesubject with a polypeptide encoded by a nucleic acid having a firstnucleotide sequence. Genomic DNA in the subject comprises a secondnucleotide sequence having one or more polymorphic variations associatedwith low BMD (e.g., the second nucleic acid has a nucleotide sequence inSEQ ID NO's:1-10). The first and second nucleotide sequences typicallyare substantially identical to one another, and the first nucleotidesequence comprises fewer polymorphic variations associated with low BMDthan the second nucleotide sequence. The first nucleotide sequence maycomprise a gene sequence that encodes a full-length polypeptide or afragment thereof. The subject is often a human.

For antibody-based therapies, antibodies can be generated that are bothspecific for target molecules and that reduce target molecule activity.Such antibodies may be administered in instances where antagonizing atarget molecule function is appropriate for the treatment ofosteoporosis.

In circumstances where stimulating antibody production in an animal or ahuman subject by injection with a target molecule is harmful to thesubject, it is possible to generate an immune response against thetarget molecule by use of anti-idiotypic antibodies (see, e.g., Herlyn,Ann. Med.; 31(1): 66-78 (1999); and Bhattacharya-Chatterjee & Foon,Cancer Treat. Res.; 94: 51-68 (1998)). Introducing an anti-idiotypicantibody to a mammal or human subject often stimulates production ofanti-anti-idiotypic antibodies, which typically are specific to thetarget molecule. Vaccines directed to osteoporosis also may be generatedin this fashion.

In instances where the target molecule is intracellular and wholeantibodies are used, internalizing antibodies may be preferred.Lipofectin or liposomes can be used to deliver the antibody or afragment of the Fab region that binds to the target antigen into cells.Where fragments of the antibody are used, the smallest inhibitoryfragment that binds to the target antigen is preferred. For example,peptides having an amino acid sequence corresponding to the Fv region ofthe antibody can be used. Alternatively, single chain neutralizingantibodies that bind to intracellular target antigens can also beadministered. Such single chain antibodies can be administered, forexample, by expressing nucleotide sequences encoding single-chainantibodies within the target cell population (see, e.g., Marasco et al.,Proc. Natl. Acad. Sci. USA 90: 7889-7893 (1993)).

Modulators can be administered to a patient at therapeutically effectivedoses to treat osteoporosis. A therapeutically effective dose refers toan amount of the modulator sufficient to result in amelioration ofsymptoms of osteoporosis. Toxicity and therapeutic efficacy ofmodulators can be determined by standard pharmaceutical procedures incell cultures or experimental animals, e.g., for determining the LD₅₀(the dose lethal to 50% of the population) and the ED₅₀ (the dosetherapeutically effective in 50% of the population). The dose ratiobetween toxic and therapeutic effects is the therapeutic index and itcan be expressed as the ratio LD₅₀/ED₅₀. Modulators that exhibit largetherapeutic indices are preferred. While modulators that exhibit toxicside effects can be used, care should be taken to design a deliverysystem that targets such molecules to the site of affected tissue inorder to minimize potential damage to uninfected cells, thereby reducingside effects.

Data obtained from cell culture assays and animal studies can be used informulating a range of dosages for use in humans. The dosage of suchcompounds lies preferably within a range of circulating concentrationsthat include the ED₅₀ with little or no toxicity. The dosage can varywithin this range depending upon the dosage form employed and the routeof administration utilized. For any compound used in the methodsdescribed herein, the therapeutically effective dose can be estimatedinitially from cell culture assays. A dose can be formulated in animalmodels to achieve a circulating plasma concentration range that includesthe IC₅₀ (i.e., the concentration of the test compound that achieves ahalf-maximal inhibition of symptoms) as determined in cell culture. Suchinformation can be used to more accurately determine useful doses inhumans. Levels in plasma can be measured, for example, by highperformance liquid chromatography.

Another example of effective dose determination for an individual is theability to directly assay levels of “free” and “bound” compound in theserum of the test subject. Such assays may utilize antibody mimicsand/or “biosensors” that have been created through molecular imprintingtechniques. Molecules that modulate target molecule activity are used asa template, or “imprinting molecule”, to spatially organizepolymerizable monomers prior to their polymerization with catalyticreagents. The subsequent removal of the imprinted molecule leaves apolymer matrix which contains a repeated “negative image” of thecompound and is able to selectively rebind the molecule under biologicalassay conditions. A detailed review of this technique can be seen inAnsell et al., Current Opinion in Biotechnology 7: 89-94 (1996) and inShea, Trends in Polymer Science 2: 166-173 (1994). Such “imprinted”affinity matrixes are amenable to ligand-binding assays, whereby theimmobilized monoclonal antibody component is replaced by anappropriately imprinted matrix. An example of the use of such matrixesin this way can be seen in Vlatakis, et al., Nature 361: 645-647 (1993).Through the use of isotope-labeling, the “free” concentration ofcompound which modulates target molecule expression or activity readilycan be monitored and used in calculations of IC₅₀. Such “imprinted”affinity matrixes can also be designed to include fluorescent groupswhose photon-emitting properties measurably change upon local andselective binding of target compound. These changes readily can beassayed in real time using appropriate fiberoptic devices, in turnallowing the dose in a test subject to be quickly optimized based on itsindividual IC₅₀. An example of such a “biosensor” is discussed in Krizet al., Analytical Chemistry 67: 2142-2144 (1995).

The examples set forth below are intended to illustrate but not limitthe invention.

EXAMPLES

In the following studies a group of subjects were selected according tospecific parameters relating to low BMD. Nucleic acid samples obtainedfrom individuals in the study group were subjected to genetic analysis,which identified associations between low BMD and certain polymorphicvariants in CETP, PROL4, GRID2, PDE4D and GPX3 (herein referred to as“target genes”, “target nucleotides”, “target polypeptides” or simply“targets”). Methods are described for producing CETP, PROL4, GRID2,PDE4D and GPX3 polypeptides and polypeptide variants in vitro or invivo. CETP, PROL4, GRID2, PDE4D and GPX3 nucleic acids or polypeptidesand variants thereof are utilized for screening test molecules for thosethat interact with CETP, PROL4, GRID2, PDE4D and GPX3 molecules. Testmolecules identified as being interactors with target polypeptides canbe screened further as osteoporosis therapeutics.

Example 1 Samples and Pooling Strategies

Sample Selection

Blood samples were collected from individuals with low BMD, which werereferred to as case samples. Also, blood samples were collected fromindividuals with high BMD (i.e., not diagnosed with osteoporosis or anyform of osteoporosis-related disease); these samples served as genderand age-matched controls. All of the samples were of Caucasian (English)descent A database was created that listed all phenotypic traitinformation gathered from individuals for each case and control sample.Genomic DNA was extracted from each of the blood samples for geneticanalyses.

DNA Extraction from Blood Samples

Six to ten milliliters of whole blood was transferred to a 50 ml tubecontaining 27 ml of red cell lysis solution (RCL). The tube was inverteduntil the contents were mixed. Each tube was incubated for 10 minutes atroom temperature and inverted once during the incubation. The tubes werethen centrifuged for 20 minutes at 3000×g and the supernatant wascarefully poured off. 100-200 μl of residual liquid was left in the tubeand was pipetted repeatedly to resuspend the pellet in the residualsupernatant White cell lysis solution (WCL) was added to the tube andpipetted repeatedly until completely mixed. While no incubation wasnormally required, the solution was incubated at 37° C. or roomtemperature if cell clumps were visible after mixing until the solutionwas homogeneous. 2 ml of protein precipitation was added to the celllysate. The mixtures were vortexed vigorously at high speed for 20 secto mix the protein precipitation solution uniformly with the celllysate, and then centrifuged for 10 minutes at 3000×g. The supernatantcontaining the DNA was then poured into a clean 15 ml tube, whichcontained 7 ml of 100% isopropanol. The samples were mixed by invertingthe tubes gently until white threads of DNA were visible. Samples werecentrifuged for 3 minutes at 2000×g and the DNA was visible as a smallwhite pellet. The supernatant was decanted and 5 ml of 70% ethanol wasadded to each tube. Each tube was inverted several times to wash the DNApellet, and then centrifuged for 1 minute at 2000×g. The ethanol wasdecanted and each tube was drained on clean absorbent paper. The DNA wasdried in the tube by inversion for 10 minutes, and then 1000 μl of 1×TEwas added. The size of each sample was estimated, and less TE buffer wasadded during the following DNA hydration step if the sample was smaller.The DNA was allowed to rehydrate overnight at room temperature, and DNAsamples were stored at 2-8° C.

DNA was quantified by placing samples on a hematology mixer for at least1 hour. DNA was serially diluted (typically 1:80, 1:160, 1:320, and1:640 dilutions) so that it would be within the measurable range ofstandards. 125 μl of diluted DNA was transferred to a clear U-bottommicrotitre plate, and 125 μl of 1×TE buffer was transferred into eachwell using a multichannel pipette. The DNA and 1×TE were mixed byrepeated pipetting at least 15 times, and then the plates were sealed.50 μl of diluted DNA was added to wells A5-H12 of a black flat bottommicrotitre plate. Standards were inverted six times to mix them, andthen 50 μl of 1×TE buffer was pipetted into well A1, 1000 ng/ml ofstandard was pipetted into well A2, 500 ng/ml of standard was pipettedinto well A3, and 250 ng/ml of standard was pipetted into well A4.PicoGreen (Molecular Probes, Eugene, Oreg.) was thawed and freshlydiluted 1:200 according to the number of plates that were beingmeasured. PicoGreen was vortexed and then 50 μl was pipetted into allwells of the black plate with the diluted DNA. DNA and PicoGreen weremixed by pipetting repeatedly at least 10 times with the multichannelpipette. The plate was placed into a Fluoroskan Ascent Machine(microplate fluorometer produced by Labsystems) and the samples wereallowed to incubate for 3 minutes before the machine was run usingfilter pairs 485 nm excitation and 538 nm emission wavelengths. Sampleshaving measured DNA concentrations of greater than 450 ng/l werere-measured for conformation. Samples having measured DNA concentrationsof 20 ng/μl or less were re-measured for confirmation.

Pooling Strategies

Samples were placed into one of two groups based on BMD levels. The twogroups were made up of individuals with low BMD levels and individualswith high BMD levels. A select set of samples from each group wereutilized to generate pools, and one pool was created for each group.Each individual sample in a pool was represented by an equal amount ofgenomic DNA. For example, where 25 ng of genomic DNA was utilized ineach PCR reaction and there were 200 individuals in each pool, eachindividual would provide 125 pg of genomic DNA. Inclusion or exclusionof samples for a pool was based upon the following criteria: the samplewas derived from an individual of Caucasian paternal and maternaldescent; the database included relevant phenotype information for theindividual; case samples were derived from individuals with low BMD;control samples were derived from individuals with normal or high BMDand no history of osteoporosis or osteoporosis-related diseases; andsufficient genomic DNA was extracted from each blood sample for allallelotyping and genotyping reactions performed during the study.Phenotype information included pre- or post-menopausal, familialpredisposition, country or origin of mother and father, diagnosis withosteoporosis (date of primary diagnosis, age of individual as of primarydiagnosis, osteoporosis-related fracture), biochemical measurements ofmarkers of bone resorption (bone-specific alkaline Phosphatase, UrinaryC-telopeptide of type I collagen, serum osteocalcin), current medicationstatus (thyroid medication, hormone replacement therapy, steroid usage,bisphosphonates and cytotoxic agents for rheumatic diseases). Samplesthat met the inclusion criteria and did not meet the exclusion criteriawere added to appropriate pools based on gender and disease status.

The selection process yielded the pools set forth in Table 2, which wereused in the studies that follow. The average (mean) T-score in the Highgroup is 1.56, and in the Low group −2.1 which means that on averageindividuals in the high BMD pool are 1.56 standard deviations above theaverage BMD in young females, whereas individuals in the low BMD poolare on average 2.1 standard deviations below the normal young value.TABLE 2 Female High BMD Female Low BMD Pool size 321 319 (Number) MeanT-score 1.56 −2.10 (BMD leveles adjusted for age and BMI) Mean Age 52.952.4 (ex: years)

Example 2 Association of Polymorphic Variants with Low BMD

A whole-genome screen was performed to identify particular SNPsassociated with low BMD. As described in Example 1, two groups ofsamples were utilized, which included samples from female individualshaving low BMD (osteoporosis cases), and samples from female individualshaving high BMD levels (controls). The initial screen of each pool wasperformed in an allelotyping study, in which certain samples in eachgroup were pooled. By pooling DNA from each group, an allele frequencyfor each SNP in each group was calculated. These allele frequencies werethen compared to one another. Particular SNPs were considered as beingassociated with low BMD when allele frequency differences calculatedbetween case and control pools were statistically significant. SNPdisease association results obtained from the allelotyping study werethen validated by genotyping each associated SNP across all samples fromeach pool. The results of the genotyping were then analyzed, allelefrequencies for each group were calculated from the individualgenotyping results, and a p-value was calculated to determine whetherthe case and control groups had statistically significantly differencesin allele frequencies for a particular SNP. When the genotyping resultsagreed with the original allelotyping results, the SNP diseaseassociation was considered validated at the genetic level.

SNP Panel Used for Genetic Analyses

A whole-genome SNP screen began with an initial screen of approximately25,000 SNPs over each set of disease and control samples using a poolingapproach. The pools studied in the screen are described in Example 1.The SNPs analyzed in this study were part of a set of 25,488 SNPsconfirmed as being statistically polymorphic as each is characterized ashaving a minor allele frequency of greater than 10%. The SNPs in the setreside in genes or in close proximity to genes, and many reside in geneexons. Specifically, SNPs in the set are located in exons, introns, andwithin 5,000 base-pairs upstream of a transcription start site of agene. In addition, SNPs were selected according to the followingcriteria: they are located in ESTs; they are located in Locuslink orEnsemb1 genes; and they are located in Genomatix promoter predictions.SNPs in the set were also selected on the basis of even spacing acrossthe genome, as depicted in Table 3. An additional 3088 SNPs wereincluded with these 25,488 SNPs and these additional SNPs had beenchosen on the basis of gene location, with preference to non-synonymouscoding SNPs located in disease candidate genes. TABLE 3 GeneralStatistics Spacing Statistics Total # of SNPs 25,488 Median  37,058 bp #of Exonic SNPs >4,335 (17%) Minimum*  1,000 bp # SNPs with 20,776 (81%)Maximum* 3,000,000 bp   refSNP ID Gene Coverage >10,000 Mean 122,412 bpChromosome All Std Deviation 373,325 pb Coverage*Excludes outliers

Allelotyping and Genotyping Results

The genetic studies summarized above and described in more detail belowidentified allelic variants associated with low BMD. The allelicvariants identified from the SNP panel described in Table 3 aresummarized below in Table 4. TABLE 4 Low Position BMD SNP Chromosome inSEQ Contig Contig Sequence Sequence Allelic Assoc. Reference ChromosomePosition ID Nos Identification Position Identification Locus PositionVariability Allele rs1801706 16q21 57068823 50109 NT_010498 10631861NM_000078 CETP UTR AG G rs1047699 12p13 11143886 49075 NT_009714 3758682NM_007244 PROL4 Exon: CT C R120Q rs1948017 4q22 94636365 49110 NT_01635418829520 NM_001510 GRID2 Intron CT C rs1498608 5q12 59895580 49652NT_006713 8937426 NM_006203 PDE4D Intron AT T rs869975 5q23 15047523350082 NT_029289 11569308 NM_002084 GPX3 Intron AG G

Table 4 includes information pertaining to the incident polymorphicvariant associated with low BMD identified herein. Public informationpertaining to the polymorphism and the genomic sequence that includesthe polymorphism are indicated. The genomic sequences identified inTable 4 may be accessed at the http addresswww.ncbi.nih.gov/entrez/query.fcgi, for example, by using the publiclyavailable SNP reference number (e.g., rs1801706). The chromosomeposition refers to the position of the SNP within NCBI's Genome Build34, which may be accessed at the following http address:www.ncbi.nlm.nih.gov/mapview/map_search.cgi?chr=hum_chr.inf&query=. The“Contig Position” provided in Table 4 corresponds to a nucleotideposition set forth in the contig sequence, and designates thepolymorphic site corresponding to the SNP reference number. The sequencecontaining the polymorphisms also may be referenced by the “SequenceIdentification” set forth in Table 4. The “Sequence Identification”corresponds to cDNA sequence that encodes associated target polypeptides(e.g., CETP) of the invention. The position of the SNP within the cDNAsequence is provided in the “Sequence Position” column of Table 4. Also,the allelic variation at the polymorphic site and the allelic variantidentified as associated with low BMD is specified in Table 4. Allnucleotide sequences referenced and accessed by the parameters set forthin Table 4 are incorporated herein by reference.

Assay for Verifying, Allelotyping, and Genotyping SNPs

A MassARRAY® system (Sequenom, Inc.) was utilized to perform SNPgenotyping in a high-throughput fashion. This genotyping platform wascomplemented by a homogeneous, single-tube assay method (hME™ orhomogeneous MassEXTEND™ (Sequenom, Inc.)) in which two genotypingprimers anneal to and amplify a genomic target surrounding a polymorphicsite of interest. A third primer (the MassEXTEND™ primer), which iscomplementary to the amplified target up to but not including thepolymorphism, was then enzymatically extended one or a few bases throughthe polymorphic site and then terminated.

For each polymorphism, SpectroDESIGNER™ software (Sequenom, Inc.) wasused to generate a set of PCR primers and a MassEXTEND™ primer was usedto genotype the polymorphism. Table 5 shows PCR primers and Table 6shows extension primers used for analyzing polymorphisms. The initialPCR amplification reaction was performed in a 5 A1 total volumecontaining 1×PCR buffer with 1.5 mM MgCl₂ (Qiagen), 200 μM each of dATP,dGTP, dCTP, dTTP (Gibco-BRL), 2.5 ng of genomic DNA, 0.1 units ofHotStar DNA polymerase (Qiagen), and 200 nM each of forward and reversePCR primers specific for the polymorphic region of interest. TABLE 5 PCRPrimers Reference Forward Reverse SNP ID PCR primer PCR primer rs1801706ACGTTGGATGTTGTAGCAG ACGTTGGATGTCCATCTCCG AAGGCAAGCAC TACTCCTAACrs1047699 GATTACCAGAGTGGTTGCT CCTGCAGGAAGCATCATCAT C rs1948017GTTTAACAGCAACCATTGA CCCCAAAGGTATGTTAAGAG GG rs1498608GAATCCCTGTTCATTCCTT ATACCTAGGTATAACCTCGG G rs869975 AACTCACTGGTGATCCTGCTGTCTCATCCACACCACTCC G

Samples were incubated at 95° C. for 15 minutes, followed by 45 cyclesof 95° C. for 20 seconds, 56° C. for 30 seconds, and 72° C. for 1minute, finishing with a 3 minute final extension at 720° C. Followingamplification, shrimp alkaline phosphatase (SAP) (0.3 units in a 2 μlvolume) (Amersham Pharmacia) was added to each reaction (total reactionvolume was 7 μl) to remove any residual dNTPs that were not consumed inthe PCR step. Samples were incubated for 20 minutes at 37° C., followedby 5 minutes at 85° C. to denature the SAP.

Once the SAP reaction was complete, a primer extension reaction wasinitiated by adding a polymorphism-specific MassEXTEND™ primer cocktailto each sample. Each MassEXTEND™ cocktail included a specificcombination of dideoxynucleotides (ddNTPs) and deoxynucleotides (dNTPs)used to distinguish polymorphic alleles from one another. In Table 6,ddNTPs are shown and the fourth nucleotide not shown is the dNTP. TABLE6 Extend Primers Reference Extend Term SNP ID Probe Mix rs1801708CCTGGTGTCTCCTCCAGC ACT rs1047699 TGTCTTGCTGGTCTGTCCCTC ACG rs1948017CAGCAACCATTGAGGGTGAAT ACG rs1498608 CCCTAAAAACTGTTCCAGGTA CGT rs869975GGGCCTCAGTAGTTCCAGC ACT

The MassEXTEND™ reaction was performed in a total volume of 9 μl, withthe addition of 1× ThermoSequenase buffer, 0.576 units ofThermoSequenase (Amersham Pharmacia), 600 nM MassEXTEND™ primer, 2 mM ofddATP and/or ddCTP and/or ddGTP and/or ddTTP, and 2 mM of dATP or dCTPor dGTP or dTTP. The deoxy nucleotide (dNTP) used in the assay normallywas complementary to the nucleotide at the polymorphic site in theamplicon. Samples were incubated at 94° C. for 2 minutes, followed by 55cycles of 5 seconds at 94° C., 5 seconds at 52° C., and 5 seconds at 72°C.

Following incubation, samples were desalted by adding 16 μl of water(total reaction volume was 25 μl), 3 mg of SpectroCLEAN™ sample cleaningbeads (Sequenom, Inc.) and allowed to incubate for 3 minutes withrotation. Samples were then robotically dispensed using a piezoelectricdispensing device (SpectroJET™ (Sequenom, Inc.)) onto either 96-spot or384-spot silicon chips containing a matrix that crystallized each sample(SpectroCHIP® (Sequenom, Inc.)). Subsequently, MALDI-TOF massspectrometry (Biflex and Autoflex MALDI-TOF mass spectrometers (BrukerDaltonics) can be used) and SpectroTYPER RT™ software (Sequenom, Inc.)were used to analyze and interpret the SNP genotype for each sample.

Genetic Analysis

Variations identified in the target genes are provided in theirrespective genomic sequences (see SEQ ID NOs:1-4) Minor allelicfrequencies for these polymorphisms was verified as being 10% or greaterby determining the allelic frequencies using the extension assaydescribed above in a group of samples isolated from 92 individualsoriginating from the state of Utah in the United States, Venezuela andFrance (Coriell cell repositories).

Genotyping results for the allelic variant set forth in Table 4 areshown for female pools in Table 7. In Table 7, “F case” and “F control”refer to female case (low BMD) and female control (high BMD) groups, and“AF” refers to allele frequency. TABLE 7 Female Genotype Results Low BMDSNP F AF case F AF control Odds Associated Reference (low) (high)p-value Ratio Allele ra1801706 A = 0.15 A = 0.19 0.00765 0.661 G G =0.85 G = 0.81 rs1047699 C = 0.85 C = 0.78 0.000594 1.65 C T = 0.15 T =0.22 rs1948017 C = 0.14 C = 0.10 0.0188 1.51 C T = 0.86 T = 0.90rs1498608 A = 0.09 A = 0.12 0.0347 0.672 T T = 0.91 T = 0.88 rs869975 A= 0.05 A = 0.10 0.000243 0.441 G G = 0.95 G = 0.90

The single marker alleles set forth in Table 7 were consideredvalidated, since the genotyping data were significantly associated withlow BMD, and because the genotyping results agreed with the originalallelotyping results. Particularly significant associations with low BMDare indicated by a calculated p-value of less than 0.05 for genotyperesults, which are set forth in bold text.

Odds ratio results are shown in Table 7 (and other Tables below). Anodds ratio is an unbiased estimate of relative risk which can beobtained from most case-control studies. Relative risk (RR) is anestimate of the likelihood of disease in the exposed group(susceptibility allele or genotype carriers) compared to the unexposedgroup (not carriers). It can be calculated by the following equation:RR=IA/Ia

IA is the incidence of disease in the A carriers and Ia is the incidenceof disease in the non-carriers.

RR>1 indicates the A allele increases disease susceptibility.

RR<1 indicates the a allele increases disease susceptibility.

For example, RR=1.5 indicates that carriers of the A allele have 1.5times the risk of disease than non-carriers, i.e., 50% more likely toget the disease.

Case-control studies do not allow the direct estimation of IA and Ia,therefore relative risk cannot be directly estimated. However, the oddsratio (OR) can be calculated using the following equation:OR=(nDAnda)/(ndAnDa)=pDA(1−pdA)/pdA(1−pDA), or

OR=((case f)/(1−case f)/((control f)/(1−control f)), wheref=susceptibility allele frequency.

An odds ratio can be interpreted in the same way a relative risk isinterpreted and can be directly estimated using the data fromcase-control studies, i.e., case and control allele frequencies. Thehigher the odds ratio value, the larger the effect that particularallele has on the development of low BMD. Possessing an alleleassociated with a relatively high odds ratio translates to having ahigher risk of developing or having low BMD.

Example 3 CETP Region Proximal SNPs

It has been discovered that a polymorphic variation (rs1801706) in agene encoding CETP is associated with the occurrence of low BMD (seeExamples 1 and 2). Ninety-one additional allelic variants proximal tors1801706 were identified and subsequently allelotyped in low BMD caseand high BMD control sample sets as described in Examples 1 and 2. Thepolymorphic variants are set forth in Table 8. The chromosome positionsprovided in column four of Table 8 are based on Genome “Build 34” ofNCBI's GenBank. The “genome letter” corresponds to the particular allelethat appears in NCBI's build 34 genomic sequence of the region(chromosome 16: positions 56743155-56840953), and the “deduced iupac”corresponds to the single letter IUPAC code for the CTEP polymorphicvariants as they appear in SEQ ID NO:1. Also, the “genome letter” maydiffer from the alleles (A1/A2) provided in Table 8 (and in subsequentTables that provide the same information) if the genome letter is on onestand and the alleles are on the complementary strand, thus havingdifferent strand orientations (i.e., reverse vs forward). TABLE 8Position in Chromosome Alleles Genome Deduced dbSNP SEQ ID NO: 1Chromosome Position (A1/A2) letter iupac 7500979 205 16 56743155 g/a g R2217332 1595 16 56744545 g/a g R 8044804 2650 16 56745600 c/t a R2270835 5496 16 56748446 c/t t Y 2133783 5782 16 56748732 g/a a R 2476095908 16 56748858 c/t g R 952440 7552 16 56750502 g/a a R 881598 9191 1656752141 t/c g R 2291955 10127 16 56753077 g/a t Y 2518054 10345 1656753295 g/a g R 866038 10399 16 56753349 t/c c Y 1436425 12028 1656754978 g/a a R 173537 13355 16 56756305 a/g t Y 247611 13687 1656756637 a/g g R 166017 14328 16 56757278 t/c c Y 173538 14746 1656757696 c/t t Y 193694 14996 16 56757946 t/c c Y 7205692 19361 1656762311 g/a a R 8048746 21775 16 56764725 g/a a R 247618 23250 1656766200 g/a g R 183130 23810 16 56766760 c/t c Y 6499863 24464 1656767414 g/a g R 4783961 27341 16 56770291 g/a g R 3816117 28605 1656771555 c/t t Y 711752 28658 16 56771608 g/a g R 708272 28735 1656771685 c/t g R 1864163 29680 16 56772630 g/a g R 4369653 29998 1656772948 g/a c Y 1864165 32521 16 56775471 c/t c Y 891141 36170 1656779120 t/g g K 891143 36427 16 56779377 c/t t Y 7205804 37336 1656780286 g/a g R 5885 37718 16 56780668 c/t c Y 1532625 37748 1656780698 a/g c Y 1532624 37926 16 56780876 t/g c M 289712 38752 1656781702 g/a c Y 7499892 39037 16 56781987 c/t c Y 5883 39800 1656782750 c/t c Y 289714 39898 16 56782848 c/t g R 158480 40674 1656783624 c/t g R 289717 41835 16 56784785 c/t g R 4344729 42325 1656785275 c/t g R 289718 42379 16 56785329 a/g c Y 289719 42388 1656785338 g/a t Y 2033254 42432 16 56785382 c/t t Y 4784744 43632 1656786582 g/a g R 291044 43899 16 56786849 c/t g R 8053613 44273 1656787223 c/t c Y 5881 44459 16 56787409 a/g g R 5880 47538 16 56790488c/g g S 7198026 47692 16 56790642 c/t t Y 5882 48539 16 56791489 g/a g R8045701 48749 16 56791699 c/t t Y 289741 49921 16 56792871 a/g g R1801706 50109 16 56793059 a/g g R 289742 50209 16 56793159 c/g c S289743 50243 16 56793193 c/t g R 289746 52652 16 56795602 g/a c Y 17233755195 16 56798145 c/t t Y 289747 56385 16 56799335 a/g c Y 1566439 5710916 56800059 a/g t Y 7205459 57618 16 56800568 c/t t Y 289749 58741 1656801691 t/c g R 289751 59222 16 56802172 t/c g R 8059220 60771 1656803721 c/t c Y 8058353 60962 16 56803912 g/a g R 289735 62009 1656804959 a/g c Y 289737 64589 16 56807539 t/g a M 291042 66054 1656809004 g/a a R 1875236 66143 16 56809093 c/t g R 821466 67822 1656810772 a/g t Y 821465 68805 16 56811755 c/g c S 4275846 70075 1656813025 c/t g R 289707 70350 16 56813300 a/g t Y 821463 71214 1656814164 g/a t Y 289706 79549 16 56822499 c/t c Y 1167741 82760 1656825710 c/t c Y 2052880 86463 16 56829413 a/c g K 1167742 86533 1656829483 c/g c S 1183256 87019 16 56829969 a/g a R 1651665 88910 1656831860 a/c a M 1651666 88955 16 56831905 c/t c Y 4784751 89021 1656831971 c/t c Y 1651667 89056 16 56832006 a/g a R 8052091 89863 1656832813 g/a g R 1684574 89879 16 56832829 t/c t Y 1684575 90066 1656833016 t/g g K 1672865 90101 16 56833051 a/g a R 821470 91029 1656833979 g/a a R 1549669 91434 16 56834384 t/g a M 291040 93636 1656836586 t/c t Y 289754 98003 16 56840953 c/t c Y

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype the proximal SNPs of Table 8are the same methods described in Examples 1 and 2 herein. The primersand probes used in these assays are provided in Table 9 and Table 10,respectively. TABLE 9 dbSNP rs# Forward PCR primer Reverse PCR primer7500979 TTGTATATGTAGGGTCCTGC AAAATAATTCAAAACCACTG 2217332ATCCAACAACAGCTTCCCAG GGTGAAATGCTGACCTGTGT 8044804 ATAAATACAACCCAGCCCACCATTTCATTTCCCTGCACTG 2270835 CATTTACCATGTAACTGCCG GGGTTGAGTATGAACAAATG2133783 ACACTCATCCCTCCATTCTG TCCCGGGAATTGAAGGGAAT 247609GTCACTAAACTATGTATCAG GGGTAGAGTGTAAATGACAG 952440 AGTCAGTGCCTGACTTTACCCATACTGAGGCATGGAACAG 881598 ACTGCACTCCAGCCTGGGTGA TGCTCCTGGCAGCAAATATC2291955 GCTAGACATGTTTTAGCAGG CCCCTTAGTTTAAAGTAAAGC 2518054CTGATCATTCTTACCGGCAC CTAGACAGTCAACAACTGAG 866038 CAAGGAACAAAGCAAGACCCGTGCCGGTAAGAATGATCAG 1436425 GTATTCAGAGTGGTGTGTGG GGGACATGGCAGAAATTCAG173537 TCCCTGTCCTTGAACTCATC CCTCTTGGGTCTTGTAGTCG 247611AGGGTCCTCCATGATTGGAG TTTCTGGACCTGACTGGGTG 166017 TAGATGGGCTGTTTCTACTGAGGGAAGATTCCAGTAAAGC 173538 TCCCAAAGTGCTGGGATTAC GGGTTTCTCAAAGGGCTAAG193694 TCCTTCCGTGATCTCACAAC CACACCAAAAGAACTGCATG 7205692TCTTAGCCTGGGACTTTCTG AAAAGCAGCTGTGACCTAAG 8048746 CAAGTTCTTCCTCCATCCACTTCCTCTCTCTGGGCTTATC 247618 TTTTCCCCCTTTTTGGGGC TGGGTTGTCACAGCAAGGTGG183130 ATAAACGGGAAAGAAGAGAC AGGGTGGAGAATCTACAGAA 6499863GTCTTGACAGGTTTGAGGAG CATCAGACAGATCCCAACAG 4783961 GAAACATGAGTCGGGATGGCAGCTTTGGTATTGGAGCAGG 3816117 ACTAGCCCAGAGAGAGGAGT AAGAACTTGACCTTGAAGGC711752 CACAAATCCCTATACCTGGC TCGCCTTCAAGGTCAAGTTC 708272AACCTGGCTCAGATCTGAAC GCCAGGTATAGGGATTTGTG 1864163 TTAGAGGGGCTGTTGGAGAGAGAGCCTGACACCTTCCCTA 4369653 AGTATCTTGACTTTATTTGG GAAAAAAATATATGATAAAGG1864165 TAAAAGGCTAGAAGTCCACC AATTAATTCTCCCCTATAGC 891141AGGCCCAGCCTGGGAAGTTT TATCAGATGGTATCCACATG 891143 TCGTGCCCATCCTGTTAGTGCACAAGCATGCCCTGTGTGG 7205804 AGGCAGCAAGCACCACAATG ATGAACGGTGCCTGGTACAC5885 GCAAAGAGATCAACGTCATC ATGCAGACAGAAACGCACTCA 1532625ACTGCTGTCTTCTGAGGCAT CATCATGGCCGATTTTGTCC 1532624 TCTATAGACTTGCCCAACGCTACTTTGGCAAATCTCTGCC 289712 ATTCGGCTTCTGTCATCCTC GGGTCCAAAGCTTTTGTCAG7499892 TGGCTGACTGGCCTGACCAC CCCTCCATTCTGTACCACTTA 5883TACTTCTGGTTCTCTGAGCG TTGAACTCGTCTCCCATCAG 289714 ACACACACATACCACATGCCTGATGGGAGACGAGTTCAAG 158480 TGAGTAGTTGGGACTATAGG ACCCCTGTCTCTACAAAAAT289717 CTCTGAGCCCAGAGTTGATC CATTCCCTGCTCCATTTCCC 4344729GCCTGAGTTCAGAAGGGAAG GGCCGTTCTCCTGTTCTAAC 289718 GCCTGCCCAATATTGTGAGTTCTTCCCTTCTGAACTCAGG 289719 TCTTCCCTTCTGAACTCAGG GCCTGCCCAATATTGTGAGT2033254 CACAACTCACAATATTGGGC CAAAGGAACAGGACTCAGAC 4784744ATCACATGCCCCAAGAAACC CCGGCCCTTTCTTTCTTTTG 291044 AGGGCATCCCAGAACAGAACTGACTAGGTCAGGTCCCCTC 8053613 GAGTTCAGGGTAGGAATAGC GCTGTGCAAATTAGGACTCT5881 GACCCCTGTCTTCCACAGGT ATCTTGGGCATCTTGAGGCA 5880 TTTCTCTCCCCAGGATATCGCCAAGAGGCTTAAGAAGAGC 7198026 TGTTGGTGGGGAAATGTGGC ACGAAGATTCTATCTAGGCA5882 TCCAGGGAGGACTCACCATG TGACTGCAGGAAGCTCTGG 8045701TTGATACTTAGCGGTCCTGG TAATATTCTGCAGGTAAACC 289741 TCTACCAGCTTGGCTCCCTCAAGGGAGGGGCAGTAGGAGA 1801706 GTAGCAGAAGGCAAGCAC AGGGAGGAGTTGGGAGCC289742 ACTGGTGAGACAATCCCTTC CCACTGGCATTAAAGTGCTG 289743TGAAAAGAGGTGGACGGCAC AGTCCTTCTTCTGTGGCTGG 289746 GAGGCTTACCAAAATGGGACAGAGCTTCTAGGCTTGGATG 172337 AGCTGGACTTTGAGGATGGC AAGGAGAGGAGGGACTGGAG289747 GTTGTTTAGGCCAAAAAGTC CAATTACGGAAGTTACACTG 1566439AAGCCCATGGCTTTTCAAGG AGATCCTGGAGCCTCATTGT 7205459 GCAGAGGGGAAAGATCTTGGAAGAGGGTATGTGTGTTGG 289749 TGAACTAAGGACCAGGCAAG GCTCACATCCTTAGATTGCC289751 AAATGATATGGAATTATGCG TATCTTTCCAAATGTTTTAC 8059220ACCTGCCATCATGAGTGCAT TAGAGCAGGGCCTGGTGTGT 8058353 CCCTACAAGAAGGCTACATCAGTGACTGCAGTGAAGAAGG 289735 CAAGGCATGCATGCTCCTTC ATCGCCAGACCCTCAAGAAC289737 GCAAGGAAGACTGATTCGTG AGCCTCAAGTCACTGATGTC 291042TGTCTCCCCATAACCAACAC AAGAAACAGCTCCCAGTCTG 1875236 GAGCTGAGTGATTCTTGTCCAATATGGTCAACTTGGTGGC 821466 AAGGAAGGAAGTGAGGGATG GGAAAGCAAAGGAATGGCAG821465 CACTGTTTACAATAGCCAAG TGGAATTACAGGCGTGAGCT 4275846GCAGCTGAGCAAAGATATGG TGGACCTCTTCTATCATCAC 289707 CTCCTTCCTTCTGCTATCTCACCAAGATTCAGGCACTGAG 821463 CGTCTGCTGGGACACTGAAA GCACCTGGAAGATTTATGGG289706 TTAAGTCCTTTACCAAAGGG TGAATGTGGCTTTACCAAGG 1167741GCCCTATTTACGTGGATTTG GCTCTGATTGTGTCTCTGTG 2052880 CATCAAGACTCCATGGAGAGAGTCAAGAACCAGTCCCTAC 1167742 CCCTAGAAACTCCCTTATCC TGAGTGGGGTACAGATGAAC1183256 AGCTCAACTCCCCCCAAGTT GAAGATAGGTGAGTTGAGGG 1651665AAGTGAGAACAGGCTTCCTG TCAAGGCTTTAGCTTGTCCC 1651666 TCTAACCTGGAACCCCTCAGATGAAGCCTGACACTTTGGG 4784751 CAAAGTGTCAGGCTTCATGG AGCTGTGGGTGAGTGTCAAG1651667 TCCCGACTTGTACACCTTGG AGAAAATCAGCCAAAGGCTC 8052091AGTCTTGCAGTCCAGGATG TTCCCTGATGGACAGATGGC 1684574 TCTTGCAGTCCAGGATGCAGGGCCATTTAGTGCTTGGAGC 1684575 TTTCCAGTCCCTGCATGTG GAGGGGTTACCCAGAACCTT1672865 AAAGGTTCTGGGTAACCCCT CAGGCCTGAAAAAGCAAAGG 821470CCTCTTTCTGTAATATCTGG CTGGGCATTGCAGAACTGAA 1549869 CTGCAATAGTACACGTGGTGAGATGTTGCAAAGGAGGTGC 291040 TACACTTGCCCAAAGTCCAC TCTCTCTGTCAATCATGGGC289754 ACTGCTTAGGTTGGCAAAGG ATGCTTCCTTCCACAGGGAC

TABLE 10 dbSNP rs# Extend Primer Term Mix 7500979 TCAACCAATAGAAAAGGC ACG2217332 AACCTCTGGTCCTCTGGA ACG 8044804 TAAGCCTTGGTATGATAC ACG 2270835GCCGTAAATTCCATTCTTC ACG 2133783 CTGAACTTTACAGGTAACA ACG 247609AAACTATGTATCAGACAAAAGCAC ACG 952440 CCTGGAAGGCAGCTGTGG ACG 881598CAGCCTGGGTGACAGAGC ACT 2291955 GACTTTTCTAGGAAAGACTTA ACG 2518054TACCGGCACAAACAGTC ACG 866038 CCCATCTCAAAAACAAAAAC ACT 1436425GGTGTGTGGCCCATGAT ACG 173537 CATCACACCTGTCTGCCATC ACT 247611CTCCATGATTGGAGACTGACA ACT 166017 CTACTGGCAGGGAACAT ACT 173538TTACAGGCGTAAGCCAC ACG 193694 CTTAAAATCTACTCCCATACAT ACT 7205692CAAAAGAGTTAGGGGAG ACG 8048746 CATGCCAAAATCTCGCC ACG 247618TTGGGGCCCCATGTAAA ACG 183130 CTTCTGTGCAGGAGAAT ACG 6499863TTGAGGAGCAGTGGTCA ACG 4783961 GGGTCCTGCCCTAGTCC ACG 3816117GAGGGAGATGGGCTGAG ACG 711752 CAATGCAGCTAGGACCTTCT ACG 708272CTGGCTCAGATCTGAACCCTAACT ACG 1864163 GGCTGTTGGAGAGGTTGATA ACG 4369653CAGGTGCTTTTACAAACAA ACG 1864165 GTCCACCATGGCCCTCC ACG 891141CTGGGAAGTTTGCAGGG ACT 891143 AGTGTGTCCACGGCTCC ACG 7205804CCTGTGCATCCATGGAG ACG 5885 CATCTCTAACATCATGGC ACG 1532625TGAGGCATGCAGACAGAAAC ACT 1532624 CAACGCCACACAGCTTGTGA ACT 289712CAGTGGATTGTGGCCCCC ACG 7499892 AGCCCGTTGGCCTGAAC ACG 5883GTTCTCTGAGCGAGTCTT ACG 289714 CATACCACATGCCATCTGGAT ACG 158480GGGACTATAGGTATGCAC ACG 289717 TTCCAGCCCCCTACAAGTCACG ACG 4344729CAGAAGGGAAGAGGGAC ACG 289718 TATGTGCAAGGAGAGAG ACT 289719CTGTGATGCCTCTCTCC ACG 2033254 GGCAGGCCCCTGACCGGC ACG 4784744CCAAGAAACCACTGAAC ACG 291044 AGTATTTAAAGGAGAGACACAC ACG 8053613GATGAGAAACTGAGGCC ACG 5881 TTCCACAGGTTGTCGGC ACT 5880ATATCGTGACTACCGTCCAG ACT 7198026 GAAATGTGGCCCCTTTC ACG 5882CAGAGCAGCTCCGAGTCC ACT 8045701 CCTCTCCCTGCTGGTGG ACG 289741TGGGAGTCAGCCCAGCTC ACT 1801706 CCTGGTGTCTCCTCCAGC ACT 289742GCCACAGAAGAAGGACTCC ACT 289743 TGAGACAATCCCTTCCCCC ACG 289746TACCAAAATGGGACTGACCTC ACG 172337 TTTGAGGATGGCCCCTGACACG ACG 289747TAGGCCAAAAAGTCTAAATTGC ACT 1566439 CCTTAGAGAAACGGAAGGTG ACT 7205459ACTTTATTCCTGGATCAGA ACG 289749 CAGGCAAGCTAATGCAA ACT 289751TGGAATTATGCGTTAAAGG ACT 8059220 TGCATTCAGCTTCTCCA ACG 8058353CTACATCTGCCTCTCTATC ACG 289735 GAGGGACAGAAGGGACC ACT 289737CTTACTTGTGTGGTTTAGAAT ACT 291042 CCCATAACCAACACTCAGCT ACG 1875236GATTCTTGTCCTCTGAGAGC ACG 821466 GAGGACAGGAGTGGAGCC ACT 821465ACAGAGAAAATGTGGCA ACT 4275846 GATATGGTTTCAAAAGCCT ACG 289707TGCCATTCTCTAGGCCCGTC ACT 821463 TGGGACACTGAAACCAGGG ACG 289706ACCAAAGGGATTGACTT ACG 1167741 GAACTCTGAGTTACATTCC ACG 2052880CTTCCCTGCCTATTTTATGTC ACT 1167742 CTTATCCCCCAACTCACCTT ACT 1183256TCATCTGCCCCCAGTTT ACT 1651665 AGGGGTTCCAGGTTAGACCCTT ACT 1651666AGGAAGCCTGTTCTCACTTTC ACG 4784751 TCATGGGGAAGCCCTAA ACG 1651667CTTGTACACCTTGGACTTGAC ACT 8052091 CAACAGCCAGCAGCCCC ACG 1684574GATGCAGGAAGCAGGGCC ACT 1684575 CACCTGTCTGTGGACTGGA ACT 1672865GGGTAACCCCTCAGCCCAG ACT 821470 TCTGGATCCCCAGTGCC ACG 1549669TAGTACACGTGGTGTAAGGG ACT 291040 CCAAAGTCCACCAGCCTCTACT ACT 289754CAAAGGATTTTAACTTCCTCTAG ACG

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table11. The allele frequency for the A2 allele is noted in the fifth andsixth columns for control pools and case pools, respectively, where “AF”is allele frequency. Some SNPs do not have an allele frequency disclosedbecause of failed assays. TABLE 11 Position in Low BMD dbSNP SEQ IDChromosome A1/A2 Associated rs# NO: 1 Position Allele High AF Low AFp-Value OR Allele 7500979 205 56743155 G/A G = 0.71 G = 0.72 0.6029 1.07G A = 0.29 A = 0.28 2217332 1595 56744545 G/A G = 0.85 G = 0.84 0.53900.90 A A = 0.15 A = 0.16 8044804 2650 56745600 C/T C = 0.47 C = T = 0.53T = 2270835 5496 56748446 C/T C = 0.00 C = 0.00 T = 1.00 T = 1.002133783 5782 56748732 G/A G = 0.41 G = 0.44 0.3412 1.14 G A = 0.59 A =0.56 247609 5908 56748858 C/T C = 0.70 C = 0.70 0.8844 1.02 C T = 0.30 T= 0.30 952440 7552 56750502 G/A G = 0.31 G = 0.30 0.8410 0.97 A A = 0.69A = 0.70 881598 9191 56752141 T/C T = 0.23 T = 0.25 0.4703 1.11 T C =0.77 C = 0.75 2291955 10127 56753077 G/A G = 0.00 G = 0.00 A = 1.00 A =1.00 2518054 10345 56753295 G/A G = 0.87 G = 0.91 0.0835 1.47 G A = 0.13A = 0.09 866038 10399 56753349 T/C T = 0.49 T = 0.51 0.5835 1.07 T C =0.51 C = 0.49 1436425 12028 56754978 G/A G = 0.39 G = 0.38 0.7660 0.96 AA = 0.61 A = 0.62 173537 13355 56756305 A/G A = 0.80 A = 0.81 0.80111.04 A G = 0.20 G = 0.19 247611 13687 56756637 A/G A = 0.38 A = 0.390.7529 1.04 A G = 0.62 G = 0.61 166017 14328 56757278 T/C T = 0.29 T =0.43 0.00001 1.80 T C = 0.71 C = 0.57 173538 14746 56757696 C/T C = 0.68C = 0.67 0.5702 0.93 T T = 0.32 T = 0.33 193694 14996 56757946 T/C T =0.14 T = 0.24 0.0015 2.02 T C = 0.86 C = 0.76 7205692 19361 56762311 G/AG = 0.01 G = 0.01 A = 0.99 A = 0.99 8048746 21775 56764725 G/A G = 0.00G = 0.00 A = 1.00 A = 1.00 247618 23250 56766200 G/A G = 0.28 G = 0.290.5339 1.08 G A = 0.72 A = 0.71 183130 23810 56766760 C/T C = 0.64 C =0.59 0.0720 0.80 T T = 0.36 T = 0.41 6499863 24464 56767414 G/A G = 0.83G = 0.84 0.5810 1.09 G A = 0.17 A = 0.16 4783961 27341 56770291 G/A G =0.48 G = 0.46 0.3803 0.90 A A = 0.52 A = 0.54 3816117 28605 56771555 C/TC = 0.56 C = 0.55 0.8019 0.97 T T = 0.44 T = 0.45 711752 28658 56771608G/A G = 0.59 G = 0.58 0.6887 0.95 A A = 0.41 A = 0.42 708272 2873556771685 C/T C = 0.62 C = 0.62 0.9629 1.01 C T = 0.38 T = 0.38 186416329680 56772630 G/A G = 0.74 G = 0.74 0.9938 1.00 G A = 0.26 A = 0.264369653 29998 56772948 G/A G = 0.37 G = 0.37 0.8163 0.97 G A = 0.63 A =0.63 1864165 32521 56775471 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00891141 36170 56779120 T/G T = 1.00 T = 1.00 G = 0.00 G = 0.00 89114336427 56779377 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 7205804 3733656780286 G/A G = 0.72 G = 0.78 0.0212 1.41 G A = 0.28 A = 0.22 588537718 56780668 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 1532625 3774856780698 A/G A = 0.60 A = 0.61 0.6329 1.06 A G = 0.40 G = 0.39 153262437926 56780876 T/G T = 0.64 T = 0.64 0.9807 1.00 T G = 0.36 G = 0.36289712 38752 56781702 G/A G = 0.66 G = 0.67 0.6644 1.06 G A = 0.34 A =0.33 7499892 39037 56781987 C/T C = 0.88 C = 0.88 0.9632 1.01 C T = 0.12T = 0.12 5883 39800 56782750 C/T C = 0.95 C = 0.96 0.8994 1.04 C T =0.05 T = 0.04 289714 39898 56782848 C/T C = 0.19 C = 0.23 0.0972 1.28 CT = 0.81 T = 0.77 158480 40674 56783624 C/T C = 0.59 C = 0.56 0.33150.86 T T = 0.41 T = 0.44 289717 41835 56784785 C/T C = 0.67 C = 0.720.1148 1.25 C T = 0.33 T = 0.28 4344729 42325 56785275 C/T C = 1.00 C =1.00 T = 0.00 T = 0.00 289718 42379 56785329 A/G A = A = 0.20 G = G =0.80 289719 42388 56785338 G/A G = G = 0.39 A = A = 0.61 2033254 4243256785382 C/T C = 0.34 C = 0.38 0.1947 1.17 C T = 0.66 T = 0.62 478474443632 56786582 G/A G = 0.63 G = 0.64 0.5113 1.08 G A = 0.37 A = 0.36291044 43899 56786849 C/T C = 0.65 C = 0.65 0.9666 1.01 C T = 0.35 T =0.35 8053613 44273 56787223 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 588144459 56787409 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 5880 4753856790488 C/G C = 0.09 C = 0.09 0.9339 1.02 C G = 0.91 G = 0.91 719802647692 56790642 C/T C = 0.00 C = 0.00 C T = 1.00 T = 1.00 5882 4853956791489 A/G A = 0.76 A = 0.80 0.0958 1.27 A G = 0.24 G = 0.20 804570148749 56791699 C/T C = 0.00 C = 0.00 T = 1.00 T = 1.00 289741 4992156792871 A/G A = 0.85 A = 0.86 0.5974 1.09 A G = 0.15 G = 0.14 180170650109 56793059 A/G A = 0.31 A = 0.23 0.0025 0.67 G G = 0.69 G = 0.77289742 50209 56793159 C/G C = 0.91 C = 0.92 0.6298 1.12 C G = 0.09 G =0.08 289743 50243 56793193 C/T C = 0.27 C = 0.24 0.1869 0.84 T T = 0.73T = 0.76 289746 52652 56795602 G/A G = 0.72 G = 0.72 0.8916 0.98 G A =0.28 A = 0.28 172337 55195 56798145 C/T C = 0.94 C = 0.91 0.0886 0.67 TT = 0.06 T = 0.09 289747 56385 56799335 A/G A = 0.49 A = 0.54 0.09811.22 A G = 0.51 G = 0.46 1566439 57109 56800059 A/G A = 0.66 A = 0.650.8578 0.98 G G = 0.34 G = 0.35 7205459 57618 56800568 C/T C = 0.16 C =0.10 0.0035 0.59 T T = 0.84 T = 0.90 289749 58741 56801691 T/C T = T =0.42 C = C = 0.58 289751 59222 56802172 T/C T = 0.98 T = 0.94 C = 0.02 C= 0.06 8059220 60771 56803721 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.008058353 60962 56803912 G/A G = 0.98 G = 0.95 A = 0.02 A = 0.05 28973562009 56804959 A/G A = A = 0.39 G = G = 0.61 289737 64589 56807539 T/G T= T = 0.28 G = G = 0.72 291042 66054 56809004 G/A G = 0.80 G = 0.820.4548 1.12 G A = 0.20 A = 0.18 1875236 66143 56809093 C/T C = 0.91 C =0.90 0.3106 0.80 T T = 0.09 T = 0.10 821466 67822 56810772 A/G A = 0.77A = 0.76 0.6743 0.94 G G = 0.23 G = 0.24 821465 68805 56811755 C/G C =0.93 C = 0.87 0.0041 0.54 G G = 0.07 G = 0.13 4275846 70075 56813025 C/TC = 1.00 C = 1.00 T = 0.00 T = 0.00 289707 70350 56813300 A/G A = 0.44 A= 0.46 0.6117 1.07 A G = 0.56 G = 0.54 821463 71214 56814164 G/A G =0.38 G = 0.41 0.4281 1.10 G A = 0.62 A = 0.59 289706 79549 56822499 C/TC = 0.00 C = 0.00 T = 1.00 T = 1.00 1167741 82760 56825710 C/T C = 0.45C = 0.46 0.7635 1.04 C T = 0.55 T = 0.54 2052880 86463 56829413 A/C A =0.67 A = 0.68 0.8378 1.03 A C = 0.33 C = 0.32 1167742 86533 56829483 C/GC = 0.70 C = 0.69 0.7322 0.96 G G = 0.30 G = 0.31 1183256 87019 56829969A/G A = 1.00 A = 1.00 G = 0.00 G = 0.00 1651665 88910 56831860 A/C A =0.30 A = 0.35 0.1423 1.24 A C = 0.70 C = 0.65 1651666 88955 56831905 C/TC = 0.71 C = 0.67 0.1294 0.81 T T = 0.29 T = 0.33 4784751 89021 56831971C/T C = 0.70 C = 0.73 0.1971 1.18 C T = 0.30 T = 0.27 1651667 8905656832006 A/G A = 0.44 A = 0.47 0.2902 1.13 A G = 0.56 G = 0.53 805209189863 56832813 G/A G = 1.00 G = 1.00 A = 0.00 A = 0.00 1684574 8987956832829 T/C T = 0.01 T = C = 0.99 C = 1684575 90066 56833016 T/G T =0.65 T = 0.67 0.3473 1.12 T G = 0.35 G = 0.33 1672865 90101 56833051 A/GA = 0.49 A = 0.50 0.6959 1.05 A G = 0.51 G = 0.50 821470 91029 56833979G/A G = 0.37 G = 0.38 0.8340 1.03 G A = 0.63 A = 0.62 1549669 9143456834384 T/G T = 0.51 T = 0.50 0.9075 0.99 G G = 0.49 G = 0.50 29104093636 56836586 T/C T = 0.76 T = 0.74 0.4056 0.89 C C = 0.24 C = 0.26289754 98003 56840953 C/T C = 0.70 C = 0.69 0.6508 0.94 T T = 0.30 T =0.31

Allelotyping results were considered particularly significant with acalculated p-value of less than or equal to 0.05 for allelotype results.These values are indicated in bold. The allelotyping p-values wereplotted in FIG. 1. The position of each SNP on the chromosome ispresented on the x-axis. The y-axis gives the negative logarithm (base10) of the p-value comparing the estimated allele in the case group tothat of the control group. The minor allele frequency of the controlgroup for each SNP designated by an X or other symbol on the graphs inFIG. 1 can be determined by consulting Table 11. For example, theleft-most X on the left graph is at position 56743155. By proceedingdown the table from top to bottom and across the graphs from left toright the allele frequency associated with each symbol shown can bedetermined.

To aid the interpretation, multiple lines have been added to the graph.The broken horizontal lines are drawn at two common significance levels,0.05 and 0.01. The vertical broken lines are drawn very 20 kb to assistin the interpretation of distances between SNPs. Two other lines aredrawn to expose linear trends in the association of SNPs to the disease.The light gray line (or generally bottom-most curve) is a nonlinearsmoother through the data points on the graph using a local polynomialregression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992)Local regression models. Chapter 8 of Statistical Models in S eds J. M.Chambers and T. J. Hastie, Wadsworth & Brooks/Cole). The black lineprovides a local test for excess statistical significance to identifyregions of association. This was created by use of a 10 kb slidingwindow with 1 kb step sizes. Within each window, a chi-square goodnessof fit test was applied to compare the proportion of SNPs that weresignificant at a test wise level of 0.01, to the proportion that wouldbe expected by chance alone (0.05 for the methods used here). Resultingp-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region areplotted below each graph at the appropriate chromosomal positions. Thegene boundary is indicated by the broken horizontal line. The exonpositions are shown as thick, unbroken bars. An arrow is place at the 3′end of each gene to show the direction of transcription.

Example 4 PROL4 Proximal SNPs

It has been discovered that a polymorphic variation (rs1047699) in agene encoding PROL4 is associated with the occurrence of low BMD (seeExamples 1 and 2). One hundred twenty-five additional allelic variantsproximal to rs1047699 were identified and subsequently allelotyped inlow BMD case and high BMD control sample sets as described in Examples 1and 2. The polymorphic variants are set forth in Table 12. Thechromosome position provided in column four of Table 12 is based onGenome “Build 34” of NCBI's GenBank. TABLE 12 Position in SEQ IDChromosome Alleles dbSNP NO: 2 Chromosome Position (A1/A2) genome_letterdeduced_iupac 523051 229 12 10842129 t/c g R 693620 368 12 10842268 t/ca R 2588349 583 12 10842483 g/a g R 2588350 2424 12 10844324 t/c t Y619381 3625 12 10845525 c/t c Y 3759252 3709 12 10845609 c/a g K 37592513750 12 10845650 t/a t W 2418107 4724 12 10846624 g/c g S 7303054 478112 10846681 t/c c Y 1838345 6161 12 10848061 a/g g R 620878 7097 1210848997 t/g g K 2537817 8025 12 10849925 t/c t Y 1548803 8398 1210850298 c/t c Y 667123 10144 12 10852044 g/a a R 1838346 10384 1210852284 a/g g R 2159903 11116 12 10853016 a/g g R 3944035 11132 1210853032 a/g g R 3741845 11482 12 10853382 t/c a R 2110096 14544 1210856444 c/t c Y 759055 15688 12 10857588 t/a t W 589377 17311 1210859211 g/c g S 7960194 17831 12 10859731 g/t t K 7978242 20012 1210861912 a/g g R 601051 21997 12 10863897 g/a g R 4262797 22861 1210864761 a/g a R 2215714 23470 12 10865370 a/g g R 1373434 23515 1210865415 g/a g R 2215715 23863 12 10865763 g/a g R 612456 24108 1210866008 c/a c M 612808 24138 12 10866038 t/c c Y 689118 26469 1210868369 t/c t Y 597468 27769 12 10869669 a/g g R 592864 29683 1210871583 t/c t Y 640372 30491 12 10872391 a/c c M 7966559 30745 1210872645 a/t a W 654834 31429 12 10873329 t/c t Y 4763216 31779 1210873679 c/g c S 668521 32194 12 10874094 a/c c M 669503 32441 1210874341 t/c c Y 3906864 32454 12 10874354 t/c g R 3906863 32459 1210874359 c/t a R 7957888 35151 12 10877051 a/t t W 9300230 35362 1210877262 a/t t W 7306214 35630 12 10877530 g/c g S 763839 36930 1210878830 g/c g S 2418105 37490 12 10879390 g/a g R 666841 38432 1210880332 c/t c Y 3851578 38688 12 10880588 a/g g R 7138797 39524 1210881424 t/c c Y 7295252 41580 12 10883480 a/c a M 2418106 42531 1210884431 t/c t Y 7299578 42665 12 10884565 a/g a R 621112 43038 1210884938 a/g a R 3863320 44183 12 10886083 g/a g R 1373432 46271 1210888171 a/t t W 1047699 49075 12 10890975 c/t t Y 1063193 49147 1210891047 c/t c Y 2232959 49180 12 10891080 c/g g S 2227296 50301 1210892201 a/g a R 1548804 50773 12 10892673 g/a g R 2232958 51243 1210893143 g/a c Y 2232957 51530 12 10893430 t/c a R 2232956 52107 1210894007 a/g c Y 1972571 52821 12 10894721 a/g a R 3759250 53341 1210895241 t/c a R 3759249 53376 12 10895276 c/g g S 1541525 54047 1210895947 c/t t Y 2098248 54392 12 10896292 t/c t Y 2900550 54482 1210896382 t/a a W 7302130 55468 12 10897368 c/a a M 4763583 56990 1210898890 a/g a R 4360778 57712 12 10899612 a/t a W 1607695 59667 1210901567 c/t a R 1607694 59684 12 10901584 t/c g R 2192139 62043 1210903943 a/c a M 7978300 63293 12 10905193 t/c t Y 7397871 63485 1210905385 g/t g K 4763217 63778 12 10905678 c/t t Y 2159900 64222 1210906122 a/g a R 10772370 65722 12 10907622 a/g a R 7398682 66315 1210908215 c/t c Y 2900551 66829 12 10908729 a/g a R 2900552 66966 1210908866 c/t c Y 2418214 66971 12 10908871 a/c c M 2418215 67013 1210908913 t/c c Y 965243 70375 12 10912275 a/t t W 1117548 74118 1210916018 t/a t W 1520225 75224 12 10917124 c/t c Y 1520226 75236 1210917136 t/c t Y 1520227 75246 12 10917146 g/c g S 971919 75812 1210917712 c/a a M 2159901 78968 12 10920868 c/t t Y 2159902 78998 1210920898 t/c t Y 2110099 79328 12 10921228 a/c c M 7314847 80922 1210922822 t/c t Y 7296003 81055 12 10922955 t/c c Y 4281556 81412 1210923312 g/a a R 4763219 81785 12 10923685 a/g g R 3851579 82079 1210923979 g/a a R 3851580 82087 12 10923987 g/a g R 1049119 82958 1210924858 a/g c Y 2298866 83351 12 10925251 t/g a M 2298865 83442 1210925342 c/t a R 2298864 83472 12 10925372 a/c t K 2298863 83966 1210925866 a/g t Y 3180393 84414 12 10926314 t/c t Y 2070837 86563 1210928463 c/g c S 7956204 86788 12 10928688 g/a g R 2418216 86796 1210928696 t/g t K 3741844 87634 12 10929534 g/a c Y 4262798 88530 1210930430 g/a a R 2418217 89202 12 10931102 a/g a R 2418218 89632 1210931532 t/c t Y 7137492 89697 12 10931597 t/c t Y 2110100 89723 1210931623 g/a g R 1013312 91063 12 10932963 c/g c S 4579993 91335 1210933235 c/g g S 1013313 91504 12 10933404 t/c t Y 7397106 91619 1210933519 c/t t Y 2215716 93715 12 10935615 a/g g R 2192140 93945 1210935845 t/c c Y 4763589 94235 12 10936135 t/c t Y 1468697 95851 1210937751 t/c c Y 2070837 130860 12 10972760 c/g c S 3180393 132815 1210974715 t/c a R 2298865 133778 12 10975678 c/t t Y

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype the proximal SNPs of Table 12are the same methods described in Examples 1 and 2 herein. The primersand probes used in these assays are provided in Table 13 and Table 14,respectively. TABLE 13 dbSNP Forward Reverse rs# PCR primer PCR primer523051 CTCTGCTCAGAGCATAGATG TTCCATGCATTTTAACCCCG 693820GTAGGTCAATAAAAGAGGAGG TTAGGCCGAGATTTTCAGAG 2588349 GTTCAAGAGTTTGATGTCAAGCAGGGCATCTAAATTGAACG 2588350 TGTATCTGGGACATACCATC GTTAGTACAGGGTTGATCAC619381 AAAGCATAGTTTCTCTTCAG GCATCTCTAAAGGTGATTTGG 3759252GCTGTGATTTTTGGTGAGTC TATTGTTCCCCAGTATTAGG 3759251 TTGTCCTTTCTCATTGCCACATTAGAGCTATGGACTCACC 2418107 AGGTAAATGGGCATACAGTG TGAAGACTGGAGCTATCTTC7303054 AGGGAATTGGCCCAATTTGC CCATTTACCTACTCAGCAGG 1838345CCCAGGTTCAAGTGATTCTC AATTAGCCAGGCATGGTTGC 620878 CCAGACCAAACGTTTCAATAAAAGAAGAAACGAATGTTTAC 2537817 GTCAGAATGCTGACATGTAG GGGGAAAAATGAGAATAGAC1548803 ATGGGACAATTGCAAACAGG ACTGAAATGTTCCATGTGAG 667123TAAATACAAGAAGCCCTAGG AGGGAAAGTACAACTTAGCC 1838346 GTTAGAAGGCTAGAGAGAACTTCATGAGTACTCTAGCTGG 2159903 AGTCAGGATACTCTTTAGGG GGGAAGCTTTTCTGAAGATG3944035 GGGTCTCTATGGAACAAAAG TGAGGGAAGCTTTTCTGAAG 3741845TGCTTGGTGTGTCTAACTAG CAAACAGTTAACCCTGAACC 2110096 ATCAAGAACAGGATACTGCGGGGTAGGAGATAAAGTCAGC 759055 CCCCTATCTTTTTGTTGGATG GCTCTGTTTTCATGAGGTTC589377 TTCACTGTTCAGATTGCTGG ACTCCAGTTGTAGGTAGAAG 7960194TGCTATCTTCCATGGAAGAA TTTTTTTCCCTGTATGCCTC 7978242 GACGCTAAGCATCATTAGTCTAGCCATTCTAGTGGATGTG 601051 TTTTTACGTCTGTGTGAACC CACAGAAACCGAAAACCAAC4262797 ATTTTAGTGCACCCATCACC GTCTTTAGAGACTCAAAAGGG 2215714GAGTTAAACATCAGTCGTTGT TTATTATATAACGTAGGAA 1373434 CGTTTAAGATGATAGATCTTCCTACGCAATAGGCTATTTTC 2215715 ACATGGATGGAAAGGAAGAC TATGAGCGAGAATATGTGGC612456 TATCAACAAGCCAAGATCCC CTTTAGCCTTTTGTTGGACG 612808GGCTGAGTGTTAAGTATTCC AAGGCAAACCCTTAATAGGC 689118 GAAATGTTCTGTCTTCAGGCAACGTTTCTGTGAGTGCAAG 597468 TACATGTTGAGATCCCAGAC TGCATACATTGCGAAGATTC592864 AAATTGTGTTTCTTGTAAGC AATGAACTAAATACTCCAAG 640372ATCTGAAACTCTTTGAGCGC TCCAAAATGTTCCAATGAGC 7966559 CCTAATTCTTTTCTATCGGTGGAAGCTAGAAAGCTAAGAGTG 654834 CTGTCCTTTTTCTGGCTTTC CAAACAAAATAGAGTCCAGCG4763216 TTGTAACCATAGAAAGCAGG TCCCTCTATTTTTCTACTAC 668521TGCTGCTGATGTAACTGCAG GTGCAATAGTAACTGGGCTC 669503 GTAATCCACTCATTCAGATCTACCTAGAGGAAATAAGTGG 3906864 TTTCTAACATCCACCTTCTG GGTGTGAATAAGTTGTAATCC3906863 CAGGACTGACCTCTAAAATAG ACATCCACTTTCTGTAGATC 7957888ACCTTAACAAAGTTCTGTGG GAGCCACGAAAAAAAAAATG 9300230 CCAGACAAGCTAAAATACAGCAAATACACACAGACACATC 7306214 CTATAGCACAGTAGGGTGAC TCTTCGTGTTGGGCACATTC763839 TATTCATGTAACACCGAAGG GGAACAGCAAACTGAAACAG 2418105GGAGGCATTCCATTCTTTTC GTGTATCCAAAGCTTTAGTG 666841 TAATCCCCAAGAGAGAGGACAACTAACACAGATGGCCACC 3851578 GTGTGTATTTGCCCTTCTGC GGCATGTGATAGGAATGTGC7138797 GTTCTGAAGTACATGTGCAG TTAATACCTAGGTGATGGGC 7295252CAAGTTTACAATCACAGCTG GGTAAATGTATTTGACAGTTG 2418106 CAGCCCAGAATGGCTTTGAAACTAACGATGGGTGATGAGC 7299578 ATCTGCCCCATGATCCAATC CCCCTGAAAACCTCATGTTG621112 TATGTTGGGTCACAAACCTG AGCAAGGCGTACAAAACAAC 3863320GTTGCTCACAATACTGGAGG TGTCTTCAGCAGAACTCATC 1373432 TAAGGCAGAGGGCTACTTACGCATTTTACAAGACTTAAATC G 1047699 ATTACCAGAGTGGTTGCTCCATTTCCTTCTGTCAGCCTGC 1063193 AGGCTGACAGAAGGAAATCG TCAGCAGAGACCACCAAAAC2232959 TCAGCAGAGACCACCAAAAC GGTGGTCGTTGCTGATTTTG 2227296TGAGAAGAAGACACTGAAGG ATCCCAATGATCCTCAATCC 1548804 CCCATTTTTCTTCCAATCACGGAGCAACCAGGAGTAAATA 2232958 TCTTCTCAACCTCCTTCCTC ATGACTCTTCTAGGGCCTCA2232957 TTTATAAACATGAGCAGAAC CGCATTCAACTTTATGAGAGG 2232956GCCAATTTTGCCTTACTAACC CTAGCATTAACCAGAGATAC 1972571 ATCACCAGCTGCCACTTTTCCCTCAAATTATCACCAGCCC 3759250 TTCTGCCTAGGTGGCTTATG AATCATGTCTGCATGGCACG3759249 CACACAGTGAATCATGTCTG ATGGTAGACCATCAGGTAGG 1541525TATCTGTAGACGTGCATCCC CTGACACCTGTCCTCATAAG 2098248 GGGAGAATCATGGCTTGAGTACTGCCCTTAGCAAATCC 2900550 TCAACAGTCTCCAGCTGATC ACTCTGGCTGTTGTAAGGAG7302130 TACACCTGTGAGCACTGACA CCTTTTCTCAAGGTGTGTGC 4763583AATGGTTTAGCACCATCCCC GGGAGGTGACACACACTTTT 4360778 AGGCTGCAAGTTTTCCAAACGCCTAAGCAAAGAAATGAGC 1607695 TTCTTCTCACTGACCTATTC ATCAAACAGAAACGCTGGAG1607694 ATCAAACAGAAACGCTGGAG CTCTTTTTTTGTCTGACTGGG 2192139GGTCTTTTGTTTGCTAGGAC GTAACCTGCCAAGATTGAAC 7978300 TCTGAGTCTTTTGTGGTACCTGCAGATTCAATGCAATCCC 7397871 AGGAGCTGAAACGATGGTTC CAAAATGGCATGACACTGGC4763217 ACATTGTGTCTGTGCTTCTC GCAGTCTCTTATTGTCTGAG 2159900TTACAACAAGTGCTCAAGGG GTGTCTTCTCTAACAGTGAG 10772370 GAATGACCATATGATCCAGCTAGGTATCCCTTTGATGTCC 7398682 GACAGATACCCTAACTTGATC GCAGTAAAGCTATCAATCCTG2900551 GTACCTCAACACAAAAAGGC GAGGAAATCTTTCACCTTTCC 2900552GCTAGATATACTAGGCAAGAG TGTCATCCGAAAAGATGAAC 2418214 TGTCATCCGAAAAGATGAACGCTAGATATACTAGGCAAGAG 2418215 CTATTCTGCAACTTTACCAG TAGAAGGAAGTCAATTGTTC965243 GGCTGTGTAGGTTTGTCCTC TTCATCCCATTCCATCCAGC 1117548GTGGTACATCACATTAACAG TTCCTTCTTTGTGTAGTTCG 1520225 CGTGACTCTCTGTACAGCATCAGCACTAGGCTGTGAAAAG 1520226 TAGGGTAAAATGTGCACAGC GCATTGTTAGTGGTTGCTCC1520227 TAGGGTAAAATGTGCACAGC GCATTGTTAGTGGTTGCTCC 971919TTAGTGACCTTCATAGAACC CAGGCATTCTTAGAAGACAG 2159901 CATATCTACTTGTGAACTGCGAAGGTAAAACCACTGATGC 2159902 CCCCTATAAGAGCAAAATAC GAACTTTTCAGAAAGCATCAG2110099 ATTCCTTCTCAACCCACATC ACATTACAGGAAGGCCTTTC 7314847CCTCTGCATAGATACAGTAG CTCTTATACACCCTGATTGG 7296003 TGGTTACTTAGGTTGATGCCCTACTGTATCTATGCAGAGG 4281556 GGCCTTAGGGTTTCCATAAC AAGCCTTGGAAGAGTGAGTG4763219 AGGAGAATCGCTTGAACCTG TGGAGTCTCATTCTGTCACC 3851579AGGCTTTCAAACAAAATAGG TTACGGGTCTGATAAGAAAG 3851580 AGGCTTTCAAACAAAATAGGTTACGGGTCTGATAAGAAAG 1049119 GATAACAGTGTTTCAAATGC GAAATTGCAAGCTGATTGTT2298866 TAACCAATCCCTGTCACTGG ACAAAGATGGGCACTGCAAC 2298865CCTGAGTTCATTTAGATCTC ACGGAACAACCAAACAATAG 2298864 CCAGTAAACACTGAAGAGATCGATTTGTGTCTTACTCACTGG 2298863 AGCTCTTGAAGGCAATTCTG TATGTCCTCATTGTCAACCC3180393 AACAAGGTCCACCACCTCCT TTGTGGCCTTCCTTGAGGAG 2070837ACACACCCACACAAACTCAC TGGAAAGACTGCTATTCTGC 7956204 TTTGGACAATTCTTGTAGCCTGTGAGATGTGTGAGGACAG 2418216 CCCAGGCACACATATATGAG GCCTGAGTGTAGTGAGATTC3741844 GCTGATTGCTCTGTGATACC AAGTGCAGCTGGTGATTCTG 4262798TCTAGGTACCCAAGCTCCTG AAGAGCTGAAAGGGACACTG 2418217 GCCTGGAACACTAAAGATGGCTCACTGACTCTCAGAGAAG 2418218 AAATTAGTCAGTCATGGTGG TTCAAGCAATTCTCCTGCCT7137492 TCCTCATTTACTACAGTGAC CCCATCTCTACTAAAAATAC 2110100CTGGAATTACAAAGAGAAGAG CCCATCTCTACTAAAAATAC 1013312 ATTGTGTTTGCCCACTTTCCGTGGAACATCAAGAATGAAG 4579993 ATTTGTGCTCCTTTCTACTG AGCAGAAAGAAGGAAATGAC1013313 TTCCAAACACAGCAAAGAGC TTGGTTGTATTCTGGTTGGG 7397106TCTGAAACTCAGAATGCATG ATACTCTAAACAATACAGGG 2215716 GTACTAGCAGTAGTCAGAGGGACACCACTACTTGCACATG 2192140 CCTTGGGATTTAGAAATAGGG GTAAGCAAATATCTCTGGAG4763589 TTGGCAACTGTGTAACCTTG TCAAACATACTGTTTGCTCAC 1468697GAACACAATCGCAAGTTTAAG GTTCTTCAAAATCTGCTTCC 2070837 ACACACCCACACAAACTCACTGGAAAGACTGCTATTCTGC 3180393 AACAAGGTCCACCACCTCCT TTGTGGCCTTCCTTGAGGAG2298865 CCTGAGTTCATTTAGATCTC ACGGAACAACCAAACAATAG

TABLE 14 dbSNP Extend Term rs# Primer Mix 523051 TCAGAGCATAGATGATGGCAAACT 693620 AGAGGAGGAAACCTTAATTTCT ACT 2588349 AGAGTTTGATGTCAAGGAAATG ACG2588350 TACCATCAAAAGCACATCATTC ACT 619381 GAATTTTCTTCCTTTTAGAATAGA ACG3759252 TGGTGAGTCCATAGCTCTAAT CGT 3759251 CCAGCTACTTTATGCCAGAG CGT2418107 TGGCCAGGCCAAGGATCG ACT 7303054 CAGCCTAAATTAACCGTATGTG ACT1838345 CCTGCCTGAGCCTGCCAA ACT 620878 TTCAATAATATTATAGTGAGGATG ACT2537817 GACATGTAGAAAAATTGCCTGC ACT 1548803 TTGCAAACAGGTTAAAGAGAGT ACG667123 AAGCCCTAGGGTATTGTGATT ACG 1838346 AAGGCTAGAGAGAACATTCCA ACT2159903 AGGGGTCTCTATGGAACAAAA ACT 3944035 AACAAAAGGCTTTCTTCTTCTAA ACT3741845 CAAAGGATAAAGGGAACCATC ACT 2110096 CAGGATACTGCGATACTGTC ACG759055 GTAATCTTGTTTGTTGTATTTTTC CGT 589377 CTGGTGTGTAGAAACACAACA ACT7960194 GCCCAGGGCTCAGGGAAG CGT 7978242 GCAAACCAAAACCACAGTCAC ACT 601051CCCGCTTATAAGCAAGAGCA ACG 4262797 TTATCCCTATCCTCCCTTCC ACT 2215714GCATCACTCTTTCTACGCAATA ACT 1373434 TGTTTCAAAACATCACTATGTAC ACG 2215715GGAAAGGAAGACATTATGGTAC ACG 612456 CCAAGATCCCTGAAAGGCAAA CGT 612808CTACTTTAGCCTTTTGTTGGAC ACT 689118 GCTTCATACACACACACACAT ACT 597468CCCAGACTGTGTCATTCTTC ACT 592864 GTGTTTCTTGTAAGCAGTATAC ACT 640372AAACTCTTTGAGCGCTGACAT ACT 7966559 TCTATCGGTGATTTCTGCTGT CGT 654834CACAATTGGCTTTTAAAACTCC ACT 4763216 CCATAGAAAGCAGGACTGGTT ACT 668521AAATTAGTCTAGAACTTGTGGAA ACT 669503 CCACTCATTCAGATCTACAGAA ACT 3906864ACATCCACCTTCTGTAGATCT ACT 3906863 GTGTGAATAAGTTGTAATCCAC ACG 7957888TGTGGAAATATTTGAAGACTCTT CGT 9300230 ATACAGAGAAGTAGAGGACAAA CGT 7306214CACAGTAGGGTGACTACAATTA ACT 763839 TAACACCGAAGGTTCCTCAG ACT 2418105CATTCTTTTCCAGTGTCAATCAA ACG 666841 GGACTTGGCAGCATTATTTATTA ACG 3851578CAGCTAATTGTGCTCCCTCA ACT 7138797 AAGTACATGTGCAGAATGTGC ACT 7295252ATCACAGCTGATAATGTCATAAT ACT 2418106 GAATGGCTTTGAATATGACCCA ACT 7299578CAGGTGCTATCACAACATCG ACT 621112 CACAAACCTGCCATTTTACTTT ACT 3863320AATACTGGAGGCTGGAAGAC ACG 1373432 CAGAGGGCTACTTACAAGAATT CGT 1047699TCTTGCTGGTCTGTCCCTC ACG 1063193 AGTTGACGGTGTCCTCGT ACG 2232959ATCACCGCCATCCTCCCC ACT 2227296 GAAATTCTAGTGGAAAAAG ACT 1548804AAAATTTCAATATGTTGCAGGCAG ACG 2232958 CCTCCCCTCCTGCTCTTC ACG 2232957GGACCTCACCACCTGAAAG ACT 2232956 TGCCTTACTAACCTCCAGG ACT 1972571CTGCCACTTTTCATTACAGGC ACT 3759250 TAGGTGGCTTATGGTAGACC ACT 3759249TCTGCATGGCACGCCCCT ACT 1541525 CCCTCTGTGGTCTGTGCTAA ACG 2098248GAATCATGGCTTGACTCAGG ACT 2900550 GCCCCTCACCACATCCCA CGT 7302130CCCACCCAGCAGGAAGAC CGT 4763583 CCTGCGTACTATTCTTATGAC ACT 4360778TTTTACACTCTGCTTCCCTTTT CGT 1607695 TTCTCACTGACCTATTCTATTTT ACG 1607694AGCTTCAAAAATAGAATAGGTCA ACT 2192139 CTTTGATCTCATCACTTGTTATT ACT 7978300TTCTGTTTCTGTGAAGAATGTC ACT 7397871 GCCAGGCTCACAGTCCAAA CGT 4763217TTGGACGTAGGACAAAGAACT ACG 2159900 GCTCAAGGGAGTTCTACATCT ACT 10772370GACCATATGATCCAGCAATTTC ACT 7398682 GCTTATAACAAAATATCATGTGTC ACG 2900551GCCATATATGACAAACCCACA ACT 2900552 ATATACTAGGCAAGAGATAGAAA ACG 2418214CTTCCTTCTATTCAATTTCAATTT ACT 2418215 CTATAAATAAGATCATGTCATCC ACT 965243CAGACCCATCAGAGAAGTAC CGT 1117548 CCAAATAAGCATATGAATAGATG CGT 1520225CAGCATTGTTAGTGGTTGCTC ACG 1520226 GCTGTGAAAAGCTATGTATGC ACT 1520227CAGCACTAGGCTGTGAAAAG ACT 971919 ATAAGATAGGTCTGTTGGAAAG CGT 2159901TGATTTCTTCAGCCATTATCCA ACG 2159902 TTAAGCTTGCATGAAAGAAGGT ACT 2110099TTATGTCAGCAGAAAAACAGAAA ACT 7314847 GAGTTTTAGTTCTTTGAGAAATC ACT 7296003GGTTGATGCCATATCTTTGCT ACT 4281556 GGTTTCCATAACAAGAATAACAAA ACG 4763219CCGATATCTTGCCACTGCAG ACT 3851579 CAAAATAGGAACACACTGAGTAT ACG 3851580CTTTCAAACAAAATAGGAACACA ACG 1049119 TGGCATCATGCTCTAACTTCA ACT 2298866CCTGTCACTGGATATTAAGGC ACT 2298865 CATTTAGATCTCTTCAGTGTTTA ACG 2298864CTGAAGAGATCTAAATGAACTCA ACT 2298863 CAATTCTGATTTTGAGAATCACT ACT 3180393GCCACAAGGACCACCCCA ACT 2070837 CCACACAAACTCACATACACA ACT 7956204AGGCTGCCAGCACCTTTCT ACG 2418216 GCACACATATATGAGAGAAAGG ACT 3741844GTGATACCTAGAAATTCCCTG ACG 4262798 CGTCTCCCTTTCACCCACC ACG 2418217TGCCAGGAAAGATGAACATTG ACT 2418218 CAGTCATGGTGGCGAGGG ACT 7137492GCCATGTGACTTAATTTCTTTAG ACT 2110100 TCCTCATTTACTACAGTGACC ACG 1013312GCCCACTTTCCCCCTTTCT ACT 4579993 ACTGTTATTTCTGGTTTCTGGT ACT 1013313ACAGCAAAGAGCATAAACCTAC ACT 7397106 CTCAGAATGCATGAATAGTACA ACG 2215716CGTACTATTTCTTCAAGCTTCA ACT 2192140 TAGGGTATCCCTCTTGGTCA ACT 4763589CTGTGTAACCTTGATCTTGGG ACT 1468697 CAATCGCAAGTTTAAGGTAACA ACT 2070837CCACACAAACTCACATACACA ACT 3180393 GCCACAAGGACCACCCCA ACT 2298865CATTTAGATCTCTTCAGTGTTTA ACG

Genetic Assay

Allelotyping results are shown for female cases and controls in Table15. The allele frequency for A2 allele is noted in the fifth and sixthcolumns for control pools and case pools, respectively, where “AF” isallele frequency. Some SNPs do not have an allele frequency disclosedbecause of failed assays. TABLE 15 Female Allelotyping Results Positionin Low BMD dbSNP SEQ ID Chromosome A1/A2 Controls (high Cases (LowAssociated rs# NO: 2 Position Allele BMD) AF BMD) AF p-Value OR Allele523051 229 10842129 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 693620 36810842268 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 2588349 583 10842483G/A G = 1.00 G = 1.00 A = 0.00 A = 0.00 2588350 2424 10844324 T/C T =0.34 T = 0.28 0.0295 0.76 C C = 0.66 C = 0.72 619381 3625 10845525 C/T C= 0.86 C = 0.91 0.0065 1.67 C T = 0.14 T = 0.09 3759252 3709 10845609C/A C = 1.00 C = 1.00 A = 0.00 A = 0.00 3759251 3750 10845650 T/A T =0.01 T = 0.01 A = 0.99 A = 0.99 2418107 4724 10846624 G/C G = 1.00 G =1.00 C = 0.00 C = 0.00 7303054 4781 10846681 T/C T = 0.00 T = 0.00 C =1.00 C = 1.00 1838345 6161 10848061 A/G A = A = G = G = 620878 709710848997 T/G T = 0.07 T = 0.03 0.0098 0.37 G G = 0.93 G = 0.97 25378178025 10849925 T/C T = 0.19 T = 0.16 0.4485 0.85 C C = 0.81 C = 0.841548803 8398 10850298 C/T C = 0.47 C = 0.41 0.0564 0.78 T T = 0.53 T =0.59 667123 10144 10852044 G/A G = 1.00 G = 1.00 A = 0.00 A = 0.001838346 10384 10852284 A/G A = 0.76 A = 0.80 0.1329 1.26 A G = 0.24 G =0.20 2159903 11116 10853016 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.003944035 11132 10853032 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 374184511482 10853382 T/C T = 0.54 T = 0.49 0.0877 0.81 C C = 0.46 C = 0.512110096 14544 10856444 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 75905515688 10857588 T/A T = 0.45 T = 0.37 0.0084 0.72 A A = 0.55 A = 0.63589377 17311 10859211 G/C G = 0.01 G = 0.00 C = 0.99 C = 1.00 796019417831 10859731 G/T G = 1.00 G = 1.00 T = 0.00 T = 0.00 7978242 2001210861912 A/G A = 0.62 A = 0.68 0.0611 1.27 A G = 0.38 G = 0.32 60105121997 10863897 G/A G = 0.01 G = 0.00 A = 0.99 A = 1.00 4262797 2286110864761 A/G A = 0.61 A = 0.54 0.0391 0.76 G G = 0.39 G = 0.46 221571423470 10865370 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 1373434 2351510865415 G/A G = 1.00 G = 1.00 A = 0.00 A = 0.00 2215715 23863 10865763G/A G = 1.00 G = 1.00 A = 0.00 A = 0.00 612456 24108 10866008 C/A C =0.03 C = 0.02 A = 0.97 A = 0.98 612808 24138 10866038 T/C T = 0.20 T =0.11 0.0001 0.52 C C = 0.80 C = 0.89 689118 26469 10868369 T/C T = 0.68T = 0.71 0.3485 1.15 T C = 0.32 C = 0.29 597468 27769 10869669 A/G A =0.99 A = 1.00 G = 0.01 G = 0.00 592864 29683 10871583 T/C T = 0.10 T =0.11 0.9292 1.04 T C = 0.90 C = 0.89 640372 30491 10872391 A/C A = 0.96A = 0.96 0.6780 1.21 A C = 0.04 C = 0.04 7966559 30745 10872645 A/T A =0.79 A = T = 0.21 T = 654834 31429 10873329 T/C T = 0.04 T = 0.03 0.39220.74 C C = 0.96 C = 0.97 4763216 31779 10873679 C/G C = 0.50 C = 0.450.0951 0.82 G G = 0.50 G = 0.55 668521 32194 10874094 A/C A = 0.98 A =0.99 C = 0.02 C = 0.01 669503 32441 10874341 T/C T = 0.96 T = 0.960.4685 1.26 T C = 0.04 C = 0.04 3906864 32454 10874354 T/C T = 0.99 T =1.00 C = 0.01 C = 0.00 3906863 32459 10874359 C/T C = 0.34 C = 0.410.0288 1.35 C T = 0.66 T = 0.59 7957888 35151 10877051 A/T A = 0.87 A =0.93 0.0155 2.12 A T = 0.13 T = 0.07 9300230 35362 10877262 A/T A = 1.00A = 1.00 T = 0.00 T = 0.00 7306214 35630 10877530 G/C G = 1.00 G = 1.00C = 0.00 C = 0.00 763839 36930 10878830 G/C G = 0.82 G = 0.88 0.00471.61 G C = 0.18 C = 0.12 2418105 37490 10879390 G/A G = 0.21 G = 0.160.0198 0.69 A A = 0.79 A = 0.84 666841 38432 10880332 C/T C = 0.89 C =0.93 0.0180 1.72 C T = 0.11 T = 0.07 3851578 38688 10880588 A/G A = 0.89A = 0.93 0.0243 1.67 A G = 0.11 G = 0.07 7138797 39524 10881424 T/C T =0.97 T = 0.97 0.9815 0.99 T C = 0.03 C = 0.03 7295252 41580 10883480 A/CA = 1.00 A = 1.00 C = 0.00 C = 0.00 2418106 42531 10884431 T/C T = 0.35T = 0.30 0.1784 0.80 C C = 0.65 C = 0.70 7299578 42665 10884565 A/G A =0.30 A = 0.23 0.0036 0.67 G G = 0.70 G = 0.77 621112 43038 10884938 A/GA = 0.87 A = 0.92 0.0086 1.69 A G = 0.13 G = 0.08 3863320 44183 10886083G/A G = 0.52 G = 0.49 0.2271 0.87 A A = 0.48 A = 0.51 1373432 4627110888171 A/T A = 0.00 A = 0.00 T = 1.00 T = 1.00 1047699 49075 10890975C/T C = 0.79 C = 0.85 0.0062 1.53 C T = 0.21 T = 0.15 1063193 4914710891047 C/T C = 0.75 C = T = 0.25 T = 2232959 49180 10891080 C/G C =1.00 C = 1.00 G = 0.00 G = 0.00 2227296 50301 10892201 A/G A = 0.77 A =0.77 0.9934 1.00 A G = 0.23 G = 0.23 1548804 50773 10892673 G/A G = 0.24G = 0.15 0.0005 0.54 A A = 0.76 A = 0.85 2232958 51243 10893143 G/A G =1.00 G = 1.00 A = 0.00 A = 0.00 2232957 51530 10893430 T/C T = 1.00 T =1.00 C = 0.00 C = 0.00 2232956 52107 10894007 A/G A = 0.87 A = 0.920.0170 1.64 A G = 0.13 G = 0.08 1972571 52821 10894721 A/G A = 0.13 A =0.13 0.8117 1.04 A G = 0.87 G = 0.87 3759250 53341 10895241 T/C T = 0.99T = 1.00 C = 0.01 C = 0.00 3759249 53376 10895276 C/G C = 1.00 C = 1.00G = 0.00 G = 0.00 1541525 54047 10895947 C/T C = 0.00 C = 0.00 T = 1.00T = 1.00 2098248 54392 10896292 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.002900550 54482 10896382 T/A T = 0.89 T = 0.93 0.0535 1.59 T A = 0.11 A =0.07 7302130 55468 10897368 C/A C = 0.36 C = 0.36 0.9550 1.01 C A = 0.64A = 0.64 4763583 56990 10898890 A/G A = A = G = G = 4360778 5771210899612 A/T A = 0.00 A = 0.01 T = 1.00 T = 0.99 1607695 59667 10901567C/T C = 0.00 C = 0.01 T = 1.00 T = 0.99 1607694 59684 10901584 T/C T =0.81 T = 0.83 0.3469 1.17 T C = 0.19 C = 0.17 2192139 62043 10903943 A/CA = 0.30 A = 0.28 0.4555 0.89 C C = 0.70 C = 0.72 7978300 63293 10905193T/C T = 0.36 T = C = 0.64 C = 7397871 63485 10905385 G/T G = 0.00 G =0.00 T = 1.00 T = 1.00 4763217 63778 10905678 C/T C = 0.89 C = 0.900.8698 1.05 C T = 0.11 T = 0.10 2159900 64222 10906122 A/G A = 0.02 A =0.02 G = 0.98 G = 0.98 10772370 65722 10907622 A/G A = 0.31 A = 0.270.1478 0.82 G G = 0.69 G = 0.73 7398682 66315 10908215 C/T C = 1.00 C =1.00 T = 0.00 T = 0.00 2900551 66829 10908729 A/G A = 0.00 A = 0.01 G =1.00 G = 0.99 2900552 66966 10908866 C/T C = 0.61 C = 0.59 0.5150 0.92 TT = 0.39 T = 0.41 2418214 66971 10908871 A/C A = 0.51 A = 0.57 0.05651.27 A C = 0.49 C = 0.43 2418215 67013 10908913 T/C T = 0.82 T = 0.860.1232 1.29 T C = 0.18 C = 0.14 965243 70375 10912275 A/T A = 0.82 A =0.85 0.1637 1.25 A T = 0.18 T = 0.15 1117548 74118 10916018 T/A T = 1.00T = 0.99 A = 0.00 A = 0.01 1520225 75224 10917124 C/T C = 0.27 C = 0.220.0956 0.79 T T = 0.73 T = 0.78 1520226 75236 10917136 T/C T = 0.99 T =0.98 C = 0.01 C = 0.02 1520227 75246 10917146 G/C G = 0.91 G = 0.820.00001 0.46 C C = 0.09 C = 0.18 971919 75812 10917712 C/A C = 0.00 C =0.00 A = 1.00 A = 1.00 2159901 78968 10920868 C/T C = 0.99 C = 0.99 T =0.01 T = 0.01 2159902 78998 10920898 T/C T = 0.54 T = 0.51 0.3599 0.90 CC = 0.46 C = 0.49 2110099 79328 10921228 A/C A = 0.60 A = 0.63 0.40801.11 A C = 0.40 C = 0.37 7314847 80922 10922822 T/C T = 0.51 T = 0.490.6059 0.94 C C = 0.49 C = 0.51 7296003 81055 10922955 T/C T = 0.63 T =0.65 0.4799 1.09 T C = 0.37 C = 0.35 4281556 81412 10923312 G/A G = 0.85G = 0.87 0.5327 1.17 G A = 0.15 A = 0.13 4763219 81785 10923685 A/G A =A = G = G = 3851579 82079 10923979 G/A G = G = 0.34 A = A = 0.66 385158082087 10923987 G/A G = 0.56 G = 0.54 0.5618 0.93 A A = 0.44 A = 0.461049119 82958 10924858 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 229886683351 10925251 T/G T = 0.72 T = 0.69 0.3240 0.87 G G = 0.28 G = 0.312298865 83442 10925342 C/T C = 0.95 C = 0.97 0.1804 1.67 C T = 0.05 T =0.03 2298864 83472 10925372 A/C A = 0.36 A = 0.34 0.5936 0.93 C C = 0.64C = 0.66 2298863 83966 10925866 A/G A = 1.00 A = 1.00 G = 0.00 G = 0.003180393 84414 10926314 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 207083786563 10928463 C/G C = 0.43 C = 0.43 0.9403 0.99 C G = 0.57 G = 0.577956204 86788 10928688 G/A G = 0.86 G = A = 0.14 A = 2418216 8679610928696 T/G T = 1.00 T = 1.00 G = 0.00 G = 0.00 3741844 87634 10929534G/A G = 0.50 G = 0.48 0.3827 0.90 A A = 0.50 A = 0.52 4262798 8853010930430 G/A G = 0.60 G = 0.62 0.4130 1.10 G A = 0.40 A = 0.38 241821789202 10931102 A/G A = 0.53 A = 0.52 0.6562 0.95 G G = 0.47 G = 0.482418218 89632 10931532 T/C T = T = C = C = 7137492 89697 10931597 T/C T= 0.75 T = 0.76 0.7043 1.07 T C = 0.25 C = 0.24 2110100 89723 10931623G/A G = 0.53 G = 0.52 0.5403 0.93 A A = 0.47 A = 0.48 1013312 9106310932963 C/G C = 0.29 C = 0.26 0.1928 0.84 G G = 0.71 G = 0.74 457999391335 10933235 C/G C = 0.00 C = 0.00 G = 1.00 G = 1.00 1013313 9150410933404 T/C T = 0.61 T = 0.58 0.3893 0.90 C C = 0.39 C = 0.42 739710691619 10933519 C/T C = 0.02 C = 0.04 T = 0.98 T = 0.96 2215716 9371510935615 A/G A = 0.45 A = 0.36 0.0372 0.69 G G = 0.55 G = 0.64 219214093945 10935845 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 4763589 9423510936135 T/C T = 0.53 T = 0.52 0.6806 0.95 C C = 0.47 C = 0.48 146869795851 10937751 T/C T = 0.02 T = 0.01 C = 0.98 C = 0.99 2070837 13086010972760 C/G C = 0.43 C = 0.43 0.9403 0.99 C G = 0.57 G = 0.57 3180393132815 10974715 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 2298865 13377810975678 C/T C = 0.95 C = 0.97 0.1804 1.67 C T = 0.05 T = 0.03

Allelotyping results were considered particularly significant with acalculated p-value of less than or equal to 0.05 for allelotype results.These values are indicated in bold. The allelotyping p-values wereplotted in FIG. 2. The position of each SNP on the chromosome ispresented on the x-axis. The y-axis gives the negative logarithm (base10) of the p-value comparing the estimated allele in the case group tothat of the control group. The minor allele frequency of the controlgroup for each SNP designated by an X or other symbol on the graphs inFIG. 2 can be determined by consulting Table 15. For example, theleft-most X on the left graph is at position 10842129. By proceedingdown the Table from top to bottom and across the graphs from left toright the allele frequency associated with each symbol shown can bedetermined.

To aid the interpretation, multiple lines have been added to the graph.The broken horizontal lines are drawn at two common significance levels,0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assistin the interpretation of distances between SNPs. Two other lines aredrawn to expose linear trends in the association of SNPs to the disease.The light gray line (or generally bottom-most curve) is a nonlinearsmoother through the data points on the graph using a local polynomialregression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992)Local regression models. Chapter 8 of Statistical Models in S eds J. M.Chambers and T. J. Hastie, Wadsworth & Brooks/Cole.). The black lineprovides a local test for excess statistical significance to identifyregions of association. This was created by use of a 10 kb slidingwindow with 1 kb step sizes. Within each window, a chi-square goodnessof fit test was applied to compare the proportion of SNPs that weresignificant at a test wise level of 0.01, to the proportion that wouldbe expected by chance alone (0.05 for the methods used here). Resultingp-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region areplotted below each graph at the appropriate chromosomal positions. Thegene boundary is indicated by the broken horizontal line. The exonpositions are shown as thick, unbroken bars. An arrow is place at the 3′end of each gene to show the direction of transcription.

Example 5 GRID2 Proximal SNPs

It has been discovered that a polymorphic variation (rs1948017) in agene encoding GRID2 is associated with the occurrence of low BMD (seeExamples 1 and 2). One hundred five additional allelic variants proximalto rs1948017 were identified and subsequently allelotyped in low BMDcase and high BMD control sample sets as described in Examples 1 and 2.The polymorphic variants are set forth in Table 16. The chromosomeposition provided in column four of Table 16 is based on Genome “Build34” of NCBI's GenBank. TABLE 16 Position in SEQ ID Chromosome dbSNP NO:3 Chromosome Position Alleles (A1/A2) genome_letter deduced_iupac1433661 206 4 94744306 t/c g R 1485009 243 4 94744343 a/g g R 76819472467 4 94746567 t/c t Y 1816432 3550 4 94747650 a/g t Y 1485018 4994 494749094 c/g g S 1485017 5167 4 94749267 g/t c M 7438397 5193 4 94749293c/t c Y 6834311 5273 4 94749373 g/a g R 1368717 5733 4 94749833 a/g c Y1017391 7817 4 94751917 a/c g K 2870701 7818 4 94751918 t/a a W 76798398612 4 94752712 t/g t K 1385404 9158 4 94753258 a/g a R 1368716 9285 494753385 g/a c Y 4693316 10680 4 94754780 g/a a R 1905707 11866 494755966 t/c c Y 1905708 11958 4 94756058 a/g a R 1905709 12044 494756144 a/t t W 3912442 12753 4 94756853 g/t t K 2082553 15585 494759685 a/g c Y 6831638 17299 4 94761399 c/t c Y 5860329 18816 494762916 —/g g N 2870702 24022 4 94768122 c/t t Y 2870703 24994 494769094 c/t t Y 1948016 26637 4 94770737 g/t t K 6835836 27635 494771735 c/g g S 1994253 28773 4 94772873 t/c t Y 1905710 29430 494773530 a/t a W 1485019 29876 4 94773976 t/a t W 978191 30364 494774464 c/t a R 1385405 31057 4 94775157 c/a t K 7694361 31782 494775882 c/t t Y 1905711 33400 4 94777500 a/c c M 1905734 35588 494779688 a/c t K 1485012 37663 4 94781763 c/g c S 1485013 37865 494781965 t/c c Y 4692981 38218 4 94782318 a/t a W 7670552 39375 494783475 t/c c Y 7670932 39559 4 94783659 c/t c Y 7688091 39833 494783933 a/g g R 7440540 40135 4 94784235 a/g g R 2171000 41698 494785798 g/a t Y 2870704 42249 4 94786349 t/c t Y 7655758 42571 494786671 g/c c S 7661436 42977 4 94787077 g/a g R 7662289 43548 494787648 t/c c Y 7667044 43631 4 94787731 g/a a R 7691929 43705 494787805 t/c t Y 5860330 43817 4 94787917 tt/t— t— N 901013 44374 494788474 a/c g K 901012 44464 4 94788564 a/c g K 901011 44788 4 94788888t/c g R 1948018 48962 4 94793062 a/g c Y 2870705 48993 4 94793093 t/g tK 1948017 49110 4 94793210 c/t a R 1905733 49434 4 94793534 c/t a R1385408 49523 4 94793623 t/g t K 1385409 49742 4 94793842 c/g c S1385410 49907 4 94794007 g/c g S 1485026 50028 4 94794128 g/c g S1485027 50089 4 94794189 g/t t K 2904483 51588 4 94795688 c/a a M1385406 52899 4 94796999 c/a a M 1905732 54088 4 94798188 a/c t K2046418 56538 4 94800638 c/t a R 2200377 59071 4 94803171 t/c g R1905731 59110 4 94803210 a/c t K 1905730 59178 4 94803278 a/g c Y 97571361087 4 94805187 t/c a R 6820985 61300 4 94805400 c/g c S 7670441 621714 94806271 t/c c Y 6810794 62783 4 94806883 t/a t W 7676623 62983 494807083 t/c c Y 1154861 63908 4 94808008 c/t t Y 1032125 64088 494808188 g/t g K 1485022 64941 4 94809041 a/t a W 1485024 65050 494809150 a/g g R 3913651 68953 4 94813053 c/t c Y 4693319 70093 494814193 t/c c Y 1872383 71308 4 94815408 c/a g K 2200376 73009 494817109 t/a t W 7668090 74002 4 94818102 a/g g R 7692930 74294 494818394 t/c t Y 967096 74879 4 94818979 g/c g S 6822249 76936 494821036 g/t t K 6532405 77195 4 94821295 g/a g R 1017897 77683 494821783 t/c c Y 7672674 78283 4 94822383 t/c t Y 7694568 78331 494822431 c/t c Y 2904484 79362 4 94823462 g/c c S 7340830 80357 494824457 c/t c Y 1485033 80653 4 94824753 t/c g R 2870706 80840 494824940 a/g g R 1905729 83203 4 94827303 a/g c Y 4693320 85405 494829505 t/c t Y 6848749 86441 4 94830541 g/t t K 6532406 86967 494831067 g/a g R 6532407 87121 4 94831221 t/c t Y 1905728 89617 494833717 t/c g R 6819866 90969 4 94835069 t/a a W 1905727 94249 494838349 g/c g S 7674069 95811 4 94839911 t/g t K 1905724 96690 494840790 t/g c M 1905723 96731 4 94840831 a/g c Y 1485020 97267 494841367 c/g c S 6814101 97414 4 94841514 t/g g K

Assay for Verifying and Allelotyping SNPs

the methods used to verify and allelotype the proximal SNPs of Table 16are the same methods described in Examples 1 and 2 herein. The primersand probes used in these assays are provided in Table 17 and Table 18,respectively. TABLE 17 DbSNP rs# Forward PCR primer Reverse PCR primer1433661 CTCCCAAAGCCATGGGATTC CAGGAATGCAATCTAAGGCC 1485009CCTAACCCTCTTCTGGAAAA GACCTATAAAGACAGGAATGC 7681947 ACCAGAACAGGACGAATTAGCCCCAATGTTATGCATACTC 1816432 GTTCTAGCCTTACTGAGATAG GCTTAAAGACCTTCAAACTAC1485018 AAAGATCGCTTGAAACTGGG TTGCTCTGTTGTCAAGGCTG 1485017TGACCAGGCTGGTCTAAAAC TAATCCCAGCAGTTTGGGAG 7438397 GAGATCTAGCAATCCTCCTGGAAACCTAATTTTGAGCCTG 6834311 TCATTGTGTTACAGATGCTC CCAGGCTCAAAATTAGGTTTC1368717 AAATCTGCAGACAAGGACAG AGCAACCTGGTTTTAAGAGC 1017391ATGCACAAATGACAGTAGGG GGACATTGTAGTAGTCAGGG 2870701 ATGCACAAATGACAGTAGGGGGACATTGTAGTAGTCAGGG 7679839 CCACAGGAACTATTTGCTGG GGAACAGCACAAGCAAAAAC1385404 CCCAGCATCCTTGCTAATAC CCTGTCAAGATGCATAGGAG 1368716TGGGAGAACTGGGAAAATGG TGCTACATACTGTAGCAGCC 4693316 ATCCTGTACTGCACTTCAGCACCCTCCATGGGAGTTTTTC 1905707 TTTTCTGTAGTTTCCCGAGC AATGCAATCCACAGAGCAAG1905708 GGAACAAATGAAGATAGATG GGACCTATGTTTTATGAGTTC 1905709TAGAATAGTTTCTCAGTGTC GAACTCATAAAACATAGGTCC 3912442 CATAACTCAGGCAAAACAACGTTGAAAAAGCTCTATATTGG 2082553 TGGAGCCCACTCAGATATTC CCCTGGAACATGTAAATGTG6831638 CCATATACTTACCTTTACTG AATGTGAGTTCTGAAGATGG 5860329CCATGCTGGCTTTTGTGAAC TCCTGACCCTTAGTCCAATG 2870702 CAAAGAATGAGAGGGAAGGCTGTGGCCTACCAGAATTTGC 2870703 AATAAAGAGGACAGCAGGAG CACAGTTGTACTCCTAGCTC1948016 GAATGAATGCAGTGTGCCAG TGAACCTATCTTGCAGGTGG 6835836TTATTTAGGAAGCACTCAGC GAGTCCAAGACTAGTAAAGAG 1994253 TTCACCTTTCTGTGTTCTCCAAGAAGAAGAACAACTGTAC 1905710 CATTAAGTGAAGAGCTATTCC CTTAAAAGCTATTCGTTAAGC1485019 TTTAAGCTCCCCAAAAGGTG TACTGTGACATTCCCTTCTC 978191AGGTCTTATCTCTTGGAATG CCCATTCAGATATATAGACAG 1385405 CATTCAGACCTGTGACAGGATGATGGGACTGTTCTTCAGG 7694361 CTAGTGATTCTGTGAATGTTG CAATGTGATTGTGTTGGAAG1905711 CAGTTAACACTCCATATCCAC GAGCCTCAGTTTGTTGAATC 1905734AATTTCAAGATCAGAGATCC CCTAACATGAGTCCATTCTG 1485012 ATACACCTATGCTAAGAAGCGCAGAAAAACTAGTACGATAC 1485013 ACACATCATAGTTTTACTGC GGATGAAACTATGTGAAAGC4692981 GCTAGCCTGGAATCAAACAC CCGAAAACAAAAGCACCCTG 7670552TTTGGCTGCAATCAACCATG ATCCCAAGAGAAATGAAAAC 7670932 CCTTGAAAGCATGCTAAGGGTGAGTCCCTATAGATTTGTC 7688091 TGGATTGTGCACTTTGGATG ACCAGACTCTCTCTTTTTTA7440540 TGCACAGACTGTTAAGGGAG TCATCCTTTAAGCCTTGCCG 2171000TGAGCCAAGTATGGAAAAAC CTGGGATAATTGTTGGGATC 2870704 ATGCTCTTGGAGCTCAGAAGACATATTTCCCTGACCTCCC 7655758 GTCAAAACCTCTTACTGTGC CATGGCAGCCTTACCAAAGA7661436 ACTGAAATTCCTTGCTGAAC TCTCCAGGTAAACTTCCAAG 7662289AAAACAGTGAAAGACCTTGC TATTGAGGCCGAGTAGTCTG 7667044 ACTGATAAAACAAAGGAGGGGAAAGCAAGGTCTTTCACTG 7691929 TTTCTCAGCATATGTTCCAG ATCAGTCTCAGGGAAGATGC5860330 CTGCCTAGATGAAACAAATG CTGCTGGAACATATGCTGAG 901013AAGGTGCATCCCTCAGAAAG GCCTGGCTGATCTAAGGTTT 901012 CTGAAATAGTGAGGGTAGTGAAGATGTGGAGAGAACAAGG 901011 AAAAGGGAAGGGAAGTGTGG ACTCTCCTCCAAGAGCTTTC1948018 TTTATCAGCAAGCATGTGAG GTTTTCTGGAAAACTTTTACC 2870705TAGTGCATGAGAAAGTCTAC TTGCTTATCTGACTGGCAAG 1948017 GTTTAACAGCAACCATTGAGGCCCCAAAGGTATGTTAAGAG 1905733 TGGAAGAGTATTAACTTCTG TTAGTCTATTGATTGAAAGC1385408 ACTCTTCCAAAGACTGATGC GGATGTGGGGTTATAAGAAC 1385409TTGCCTCTTCCAGTCTATTG CAGAGGAGAGACATGATCAG 1385410 AGAGACAGAAGAGACACTAGCCCCATCCAAATACATTGCC 1485026 GAGAAGAAGGCATATGTGAG CTCACTCTTTCTTCTGTCTG1485027 TGGTGATGATAGGTGCTGTG ACACAGGTGCTTTCTAATTG 2904483CTTCTCTCCATGAAACTCAG AGGAGATATCAAGACACCAG 1385406 CTCCATTATCTGTTTGCAGTCTCTGAGTAGTGTTTTCTGG 1905732 CTGTTCCTTGTTTCTTCTGC GTGGTGTTTGCCATTAAAAG2046418 AGGAGAGAAGTCTATGTCCC TTCTTACATTCTAGAGCCTT 2200377GGATGGACTATTGTTTTCTT CCTATCTTGATTAGATTACAG 1905731 TCACTGCTTAGAAACTAATCAAACACCTTTCTGAGAATTG 1905730 AAACTGAAGCAGATTCTGAC CTTTGTTCTTTCAATTCTCAG975713 GCTAATTTTCCTAGGATCTC AGTGAGCCCCAAAATCTGTG 6820985CTAAGCCTAATAAAGGAGGG ATATATAGAAAAGACTGGAG 7670441 CAATAATGACATGCTCTTACCGTATAGCAATCATACACAAG 6810794 ATGATGGAGCTCCAGAAAAG CAGAGATATTTAGTGGCAAC7676623 TTCAGAGCTCACTTTCCAAC GGGGCTACCAAACTTAAAAG 1154861GACACTGAATCCATAGATCG CCTTTTTAAATAACCAGTGAC 1032125 CCTCATGCTTTAATAGGTAGCACACATTTCTATAGTACTTG 1485022 AAATTAGGCTGAAGGAACAG GCTTGATGGTTATTACAATGG1485024 CGTTTCCACCACCTGGAAAT CTGGCTTTCTTCCATTGTTG 3913651CCTTGAGTATCTAAGAAAGGC AAAATATACTTGTTTTGAGC 4693319 TCAAGAGTGGAAAGGGAGAGCAGCTCCACTCACTATCTTG 1872383 TGGCCTCAAATGCATGTGTC CTTTGGAGCTATCCAAATGG2200376 GTGTTTGAGAAAGAAGTGTG GAGTTCAGACAGAGTATGAG 7668090GTAGGCCTGAGAATGCATTG GACATTCCATTATTCCTCCC 7692930 ATGAGAACACATGGACACGGTCTCCTAATGCTATCCCTCC 967096 GATTGGAAAAGGGCAACAGG TCCATGAGAATGTTCCTCAG6822249 AAAGGATGTTTCCATTTCTC CCTAGAAAGGTAGTTGATGC 6532405CTCAGGATCATTGAGACTTAG CTTGAAAGATAACTGCGGAC 1017897 TGCCCAAACTGCAAATACACGCTACATTAAGTGAATTCTAC 7672674 CTACAACAGACAAGGATGGG CAATGCCTTCAGCATTTTCC7694568 CCATTTCTAATGGGTACCTC TGCCATACACTACAACAGAC 2904484ATTTTCACATTGCTTTGCCC GAACAAGCAGAGCAAGTAAG 7340830 CAATCTCAAAACAGTGTTTACACTAAGAAAGGAGAGAACAG 1485033 CTGGCTGGAATGTTAATAGG TGTGCTCCTCAGTACATAGC2870706 CCTGCAGGAAGAAAATAGGC AGGGAAGCAAAACTAAGATG 1905729GGGAATTACTCTAGCTCTTC AGGAAAGAGTAGGCCAAATG 4693320 TCAAACTAGTAGCCCACAAAGTATAGATTACAGTAGATGTG 6848749 CCATCTTTTTGTCTGCATTC CAGTCAATTTTCATATTGCC6532406 GCATGGCTCTTAGGAGAAAG GCTGGAAGGGAAAATGGTAC 6532407TTGTTTTCCTAGGCTCCAGC TGGCTACAATAGGGAGACTG 1905728 GCAAGTTCATTTTCATATAGCTAAAACAGTGTGAAATTTTG G 6819866 CACACATAGCTCTTCTGTAAGGCCTCGAGGAAAAAAAAATAG 1905727 AAGGGATAACAAGACAAATG ACCCCATGATCTACTATTTC7674069 CAGTTACTCCAAACTTACGG GGTAACAAAGGCACTCAAGG 1905724GCTCTCAGTGTCTTTTAATG CGCATATGATTAGCTACTTC 1905723 GGGTTTATAAGCCTTTTCTTCCTTGCTAACAATGAAAAGGTG 1485020 GTTGTCATTTTATGTCCTCCG TAGAGTCACTGCCAATAAAC8814101 GAGGACTTCAATGACTTTGC TCAGACACAAACATCTGAAG

TABLE 18 dbSNP Extend Term rs# Primer Mix 1433661 CATGGGATTCCAGGTGT ACT1485009 CCTCTTCTGGAAAATCTTAA ACT 7681947 ACGAATTAGTCCAAGGA ACT 1816432TGAGATAGTTAATTTTGTTTTCCA ACT 1485018 CAGTGAGCCATGACTGT ACT 1485017GGTCTAAAACTCCTGAGAT CGT 7438397 CTCCTGCCTTGGCCTCC ACG 6834311GAAGAGAAAGAGATGGC ACG 1368717 AGGGATCGGAAATTTAAGAAGAA ACT 1017391AATGACAGTAGGGATTATAGTAA ACT 2870701 GACAGTAGGGATTATAGTAAG CGT 7679839ATTTGCTGGCCCTTTAA ACT 1385404 CCTAAAGCAGCCACTGT ACT 1368716CAATGAGAAATGCCAGC ACG 4693316 GCGATAGAGTGAGATTCTG ACG 1905707TGTAGTTTCCCGAGCTAGATT ACT 1905708 AGATAGATGAATGGAGAACCC ACT 1905709GTTTCTCAGTGTCATCAAATA CGT 3912442 AAAAAGACATATCTTCTTTTAGTG CGT 2082553CCACTCAGATATTCCATAAC ACT 6831638 CCTTTACTGTGATATTTATTTCTT ACG 5860329GCTCCTGAAACATATTCATG ACT 2870702 GAAGGCATTATGACATGAAT ACG 2870703TTGTTTATTCAAATCTGCCA ACG 1948016 GCCAGTAATCTCTCCAATGAT CGT 6835836CACTCAGCTGAATAGACA ACT 1994253 CTGACTCATACATCCTTTG ACT 1905710GAGCTATTCCAATGTGCT CGT 1485019 AAGCTCCCCAAAAGGTGTTTAATT CGT 978191TGTACAAATCTGAGGGC ACG 1385405 CCTGTGACAGGATTCCAGCA CGT 7694361GGATTACAGTAGTTTCCC ACG 1905711 CCATATCCACAGGTTCT ACT 1905734CTTAGCCACTCTGATAATCT ACT 1485012 ACACTGCAAAAAGCACT ACT 1485013TGCAGAGATAATGTATGTAGAA ACT 4692981 TCAAACACAGTTTATATGAGATAA CGT 7670552CAACCATGCTGCTATGA ACT 7670932 CATGCTAAGGGAAAGAAG ACG 7688091GGATGGGTGAATTGTATATTAT ACT 7440540 TTAAGGGAGAGCATGAAA ACT 2171000GGTAGAAATGGACTTTGA ACG 2870704 GCAGCTTCCTAACAAAAA ACT 7655758GCCTTTGAAAGAATCCAA ACT 7661436 CTTTTTTGTTTCTATCCAGG ACG 7662289TGCTTCTTTATTCCCCA ACT 7667044 GCTTTGTTTTTGATGAGTG ACG 7691929TTAATTCCTGAGACGTGT ACT 5860330 CTTGCAAGTGATTAAAAAAAAAA ACT 901013GAAAGCATTCATCTCACTA ACT 901012 GTAGTGTCTACAAAGGGTATA ACT 901011GTTGATCTCCTTCCTGG ACT 1948018 GAGAAAGTAGACTTTCTCAT ACT 2870705CTTTCTCACATGCTTGC ACT 1948017 CAGCAACCATTGAGGGTGAAT ACG 1905733TGTCCTTGACTGATTTTTAG ACG 1385408 TCTAGGAAGTATGAGATGG ACT 1385409CAATAGTAACTGTCAACTGT ACT 1385410 CAAGGAAGCTAGAGCCA ACT 1485026CTTCAATTAGAAAGCACCT ACT 1485027 GTGCTGTGAAGACAAATTA CGT 2904483GGATTGTTTCTTCCTCT CGT 1385408 TGTATACAGAAAAAAGCATGA CGT 1905732GTTGGTGCAAAAGTAACT ACT 2046418 GTCTATCTCCCTGACAC ACG 2200377AAAAATTAAAACATTCACTGCTTA ACT 1905731 TTCTGTAATCTAATCAAGATAGG ACT 1905730TCTGACAGGACTAAACAA ACT 975713 GGTCACCTAAGGATTTTACA ACT 6820985AAAGGAGGCCTCTACCC ACT 7670441 CATGCTCTTACATGCAAATA ACT 6810794CCACAAAACCAAGCTTATTA CGT 7676623 TCTTCTTTAATGTGATGGTAC ACT 1154861GAATCCATAGATCGTATACTAAT ACG 1032125 ATTTATATTCCGCCCCA CGT 1485022TCCAAAACGACCAGTCA CGT 1485024 TTCATGCTAACTGATTATCAAAT ACT 3913651GGCAAAATTCCATGGCC ACG 4693319 GAGTAATTGGACCTCTAC ACT 1872383CCATTCCATATCCTTACC CGT 2200376 TTTTACATCTCACCCCA CGT 7668090GAATGCATTGGAGTGAG ACT 7692930 ATCACACTCCGGGGACT ACT 967096AGGGCAACAGGGACACA ACT 6822249 GTATTCACCATTGCAAAAA CGT 6532405GAACAACTGAAATCTGAAGTA ACG 1017897 AACTGCAAATACACATTTCA ACT 7672674GTTTTGAACACTTAATGTTTG ACT 7694568 GATTTCACCCCTTTCCC ACG 2904484AAATGTTCTCCAAGAAAGAT ACT 7340830 GTTTACATATGAGGAAATGTAG ACG 1485033GCCCTCTAAAGACATGA ACT 2870706 GAAGAAAATAGGCTGATTTTAT ACT 1905729CTAGCTCTTCAGAATTAATTGG ACT 4693320 CCCACAAAGTCTTATGCA ACT 6848749CCAACAGAGAGAGGTATTTA CGT 6532406 CATGGTCTACACACCTTTA ACG 6532407CCTTGTAAGACTACCTGAA ACT 1905728 CAGTTAATAATTGTAGATCCATG ACT 6819866GCTCTTCTGTAAGAAGTCT CGT 1905727 ATGATTGTAGATCATTTTGATGTA ACT 7674069TTCCTAACTCTTCACCTT ACT 1905724 CATTGTTAGCAAGTGGAA ACT 1905723GAGCATAAAGATGCTCTCAGT ACT 1485020 CTCCGTTATCTCCATGT ACT 6814101GCAAATGTAGTTGTATGTAATTT ACT

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table19. The allele frequency for the A2 allele is noted in the fifth andsixth columns for control pools and case pools, respectively, where “AF”is allele frequency. Some SNPs do not have an allele frequency disclosedbecause of failed assays. TABLE 19 Position in Low BMD SEQ ID ChromosomeA1/A2 Control AF Case AF Associated dbSNP rs# NO: 3 Position Allele(High BMD) (Low BMD) p-Value OR Allele 1433661 206 94744306 T/C T = 0.11T = 0.17 0.0089 1.64 T C = 0.89 C = 0.83 1485009 243 94744343 A/G A =0.00 A = 0.00 G = 1.00 G = 1.00 7681947 2467 94746567 T/C T = T = 0.89 C= C = 0.11 1816432 3550 94747650 A/G A = 0.56 A = 0.61 0.0779 1.23 A G =0.44 G = 0.39 1485018 4994 94749094 C/G C = 1.00 C = 0.99 G = 0.00 G =0.01 1485017 5167 94749267 G/T G = G = T = T = 7438397 5193 94749293 C/TC = 0.94 C = 0.94 0.8804 0.96 C T = 0.06 T = 0.06 6834311 5273 94749373G/A G = 0.51 G = 0.56 0.1212 1.20 G A = 0.49 A = 0.44 1368717 573394749833 A/G A = 0.10 A = 0.13 0.1573 1.31 A G = 0.90 G = 0.87 10173917817 94751917 A/C A = 0.64 A = 0.63 0.6402 0.94 C C = 0.36 C = 0.372870701 7818 94751918 T/A T = 0.00 T = 0.00 A = 1.00 A = 1.00 76798398612 94752712 T/G T = 0.97 T = 0.90 0.0000 0.31 G G = 0.03 G = 0.101385404 9158 94753258 A/G A = A = 1.00 G = G = 0.00 1368716 928594753385 G/A G = 0.89 G = 0.83 0.0251 0.62 A A = 0.11 A = 0.17 469331610680 94754780 G/A G = 0.57 G = 0.53 0.2431 0.87 A A = 0.43 A = 0.471905707 11866 94755966 T/C T = 0.07 T = 0.13 0.0008 1.99 T C = 0.93 C =0.87 1905708 11958 94756058 A/G A = 0.97 A = 0.92 0.0003 0.35 G G = 0.03G = 0.08 1905709 12044 94756144 A/T A = 0.05 A = 0.06 0.8882 1.08 A T =0.95 T = 0.94 3912442 12753 94756853 G/T G = 0.00 G = 0.00 T = 1.00 T =1.00 2082553 15585 94759685 A/G A = 0.00 A = 0.11 G = 1.00 G = 0.896831638 17299 94761399 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 586032918816 94762916 —/G — = 0.00 — = 0.00 G = 1.00 G = 1.00 2870702 2402294768122 C/T C = 0.66 C = 0.65 0.8596 0.98 T T = 0.34 T = 0.35 287070324994 94769094 C/T C = 0.63 C = 0.62 0.7613 0.96 T T = 0.37 T = 0.381948016 26637 94770737 G/T G = 0.52 G = 0.47 0.1367 0.84 T T = 0.48 T =0.53 6835836 27635 94771735 C/G C = 0.00 C = 0.00 G = 1.00 G = 1.001994253 28773 94772873 T/C T = 0.92 T = 0.87 0.0134 0.61 C C = 0.08 C =0.13 1905710 29430 94773530 A/T A = 0.39 A = 0.34 0.2784 0.83 T T = 0.61T = 0.66 1485019 29876 94773976 T/A T = 0.14 T = 0.19 0.0338 1.42 T A =0.86 A = 0.81 978191 30364 94774464 C/T C = 0.00 C = 0.00 T = 1.00 T =1.00 1385405 31057 94775157 C/A C = 0.68 C = 0.66 0.6519 0.94 A A = 0.32A = 0.34 7694361 31782 94775882 C/T C = 0.21 C = 0.24 0.1629 1.22 C T =0.79 T = 0.76 1905711 33400 94777500 A/C A = 0.00 A = 0.00 C = 1.00 C =1.00 1905734 35588 94779688 A/C A = 0.92 A = 0.88 0.0229 0.61 C C = 0.08C = 0.12 1485012 37663 94781763 C/G C = 0.96 C = 0.91 0.0032 0.47 G G =0.04 G = 0.09 1485013 37865 94781965 T/C T = 0.00 T = 0.00 C = 1.00 C =1.00 4692981 38218 94782318 A/T A = 0.68 A = 0.72 0.1741 1.19 A T = 0.32T = 0.28 7670552 39375 94783475 T/C T = 0.11 T = 0.22 0.0000 2.26 T C =0.89 C = 0.78 7670932 39559 94783659 C/T C = 1.00 C = 1.00 T = 0.00 T =0.00 7688091 39833 94783933 A/G A = 0.00 A = 0.13 G = 1.00 G = 0.877440540 40135 94784235 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 217100041698 94785798 G/A G = 0.00 G = 0.00 A = 1.00 A = 1.00 2870704 4224994786349 T/C T = 0.53 T = 0.53 0.8418 1.02 T C = 0.47 C = 0.47 765575842571 94786671 G/C G = 0.21 G = 0.25 0.0531 1.32 G C = 0.79 C = 0.757661436 42977 94787077 G/A G = 0.44 G = 0.45 0.7243 1.04 G A = 0.56 A =0.55 7662289 43548 94787648 T/C T = 0.24 T = 0.29 0.1625 1.27 T C = 0.76C = 0.71 7667044 43631 94787731 G/A G = 0.18 G = 0.22 0.0669 1.31 G A =0.82 A = 0.78 7691929 43705 94787805 T/C T = 0.93 T = 0.90 0.0439 0.64 CC = 0.07 C = 0.10 5860330 43817 94787917 T/— T = 0.48 T = 0.52 0.14491.19 T — = 0.52 — = 0.48 901013 44374 94788474 A/C A = 0.00 A = 0.00 C =1.00 C = 1.00 901012 44464 94788564 A/C A = 0.00 A = 0.00 C = 1.00 C =1.00 901011 44788 94788888 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.001948018 48962 94793062 A/G A = 0.10 A = 0.19 0.0002 2.20 A G = 0.90 G =0.81 2870705 48993 94793093 T/G T = 1.00 T = 1.00 G = 0.00 G = 0.001948017 49110 94793210 C/T C = 0.12 C = 0.17 0.0068 1.60 C T = 0.88 T =0.83 1905733 49434 94793534 C/T C = 0.02 C = 0.03 0.5881 1.41 C T = 0.98T = 0.97 1385408 49523 94793623 T/G T = 1.00 T = 1.00 G = 0.00 G = 0.001385409 49742 94793842 C/G C = 1.00 C = 1.00 G = 0.00 G = 0.00 138541049907 94794007 G/C G = 0.53 G = 0.51 0.5313 0.93 C C = 0.47 C = 0.491485026 50028 94794128 G/C G = 1.00 G = 1.00 C = 0.00 C = 0.00 148502750089 94794189 G/T G = 0.00 G = 0.00 T = 1.00 T = 1.00 2904483 5158894795688 C/A C = 0.68 C = 0.65 0.4125 0.90 A A = 0.32 A = 0.35 138540652899 94796999 C/A C = 0.00 C = 0.00 A = 1.00 A = 1.00 1905732 5408894798188 A/C A = 0.48 A = 0.47 0.8078 0.97 C C = 0.52 C = 0.53 204641856538 94800638 C/T C = 0.64 C = 0.65 0.9229 1.01 C T = 0.36 T = 0.352200377 59071 94803171 T/C T = 0.00 T = 0.04 C = 1.00 C = 0.96 190573159110 94803210 A/C A = 1.00 A = 1.00 C = 0.00 C = 0.00 1905730 5917894803278 A/G A = 0.74 A = 0.73 0.9270 0.99 G G = 0.26 G = 0.27 97571361087 94805187 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 6820985 6130094805400 C/G C = 0.99 C = 0.99 G = 0.01 G = 0.01 7670441 62171 94806271T/C T = 0.67 T = 0.66 0.7167 0.96 C C = 0.33 C = 0.34 6810794 6278394806883 T/A T = 1.00 T = 1.00 A = 0.00 A = 0.00 7676623 62983 94807083T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 1154861 63908 94808008 C/T C =0.73 C = 0.70 0.2406 0.84 T T = 0.27 T = 0.30 1032125 64088 94808188 G/TG = 0.50 G = 0.51 0.9342 1.01 G T = 0.50 T = 0.49 1485022 64941 94809041A/T A = 1.00 A = 0.98 T = 0.00 T = 0.02 1485024 65050 94809150 A/G A =0.04 A = 0.12 0.0000 3.18 A G = 0.96 G = 0.88 3913651 68953 94813053 C/TC = 0.40 C = 0.41 0.6884 1.05 C T = 0.60 T = 0.59 4693319 70093 94814193T/C T = 0.81 T = 0.80 0.7260 0.95 C C = 0.19 C = 0.20 1872383 7130894815408 C/A C = 0.35 C = 0.38 0.4769 1.10 C A = 0.65 A = 0.62 220037673009 94817109 T/A T = 0.65 T = 0.63 0.6117 0.92 A A = 0.35 A = 0.377668090 74002 94818102 A/G A = 0.61 A = 0.59 0.4762 0.92 G G = 0.39 G =0.41 7692930 74294 94818394 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00967096 74879 94818979 G/C G = 0.00 G = 0.00 C = 1.00 C = 1.00 682224976936 94821036 G/T G = 0.20 G = T = 0.80 T = 6532405 77195 94821295 G/AG = 0.44 G = 0.46 0.5246 1.08 G A = 0.56 A = 0.54 1017897 77683 94821783T/C T = 0.75 T = 0.72 0.3873 0.89 C C = 0.25 C = 0.28 7672674 7828394822383 T/C T = 0.81 T = 0.82 0.6661 1.07 T C = 0.19 C = 0.18 769456878331 94822431 C/T C = 0.82 C = 0.91 0.0004 2.33 C T = 0.18 T = 0.092904484 79362 94823462 G/C G = 0.67 G = 0.66 0.8007 0.97 C C = 0.33 C =0.34 7340830 80357 94824457 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.001485033 80653 94824753 T/C T = 0.74 T = 0.73 0.6809 0.95 C C = 0.26 C =0.27 2870706 80840 94824940 A/G A = 0.65 A = 0.65 0.9576 1.01 A G = 0.35G = 0.35 1905729 83203 94827303 A/G A = 0.66 A = 0.62 0.1186 0.82 G G =0.34 G = 0.38 4693320 85405 94829505 T/C T = 0.67 T = 0.73 0.0335 1.32 TC = 0.33 C = 0.27 6848749 86441 94830541 G/T G = 0.14 G = 0.22 0.00161.68 G T = 0.86 T = 0.78 6532406 86967 94831067 G/A G = 0.98 G = 0.950.0460 0.44 A A = 0.02 A = 0.05 6532407 87121 94831221 T/C T = 0.95 T =0.91 0.0301 0.57 C C = 0.05 C = 0.09 1905728 89617 94833717 T/C T = 0.00T = 0.00 C = 1.00 C = 1.00 6819866 90969 94835069 T/A T = 0.51 T = 0.420.0157 0.70 A A = 0.49 A = 0.58 1905727 94249 94838349 G/C G = 0.06 G =0.09 0.1689 1.48 G C = 0.94 C = 0.91 7674069 95811 94839911 T/G T = 1.00T = 1.00 G = 0.00 G = 0.00 1905724 96690 94840790 T/G T = 0.06 T = 0.070.3032 1.31 T G = 0.94 G = 0.93 1905723 96731 94840831 A/G A = 0.71 A =0.70 0.7768 0.96 G G = 0.29 G = 0.30 1485020 97267 94841367 C/G C = 1.00C = 1.00 C G = 0.00 G = 0.00 6814101 97414 94841514 T/G T = T = 0.00 G G= G = 1.00

Allelotyping results were considered particularly significant with acalculated p-value of less than or equal to 0.05 for allelotype results.These values are indicated in bold. The allelotyping p-values wereplotted in FIG. 3. The position of each SNP on the chromosome ispresented on the x-axis. The y-axis gives the negative logarithm (base10) of the p-value comparing the estimated allele in the case group tothat of the control group. The minor allele frequency of the controlgroup for each SNP designated by an X or other symbol on the graphs inFIG. 3 can be determined by consulting Table 19. For example, theleft-most X on the left graph is at position 44917643. By proceedingdown the Table from top to bottom and across the graphs from left toright the allele frequency associated with each symbol shown can bedetermined.

To aid the interpretation, multiple lines have been added to the graph.The broken horizontal lines are drawn at two common significance levels,0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assistin the interpretation of distances between SNPs. Two other lines aredrawn to expose linear trends in the association of SNPs to the disease.The light gray line (or generally bottom-most curve) is a nonlinearsmoother through the data points on the graph using a local polynomialregression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992)Local regression models. Chapter 8 of Statistical Models in S eds J. M.Chambers and T. J. Hastie, Wadsworth & Brook/Cole). The black lineprovides a local test for excess statistical significance to identifyregions of association. This was created by use of a 10 kb slidingwindow with 1 kb step sizes. Within each window, a chi-square goodnessof fit test was applied to compare the proportion of SNPs that weresignificant at a test wise level of 0.01, to the proportion that wouldbe expected by chance alone (0.05 for the methods used here). Resultingp-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region areplotted below each graph at the appropriate chromosomal positions. Thegene boundary is indicated by the broken horizontal line. The exonpositions are shown as thick, unbroken bars. An arrow is place at the 3′end of each gene to show the direction of transcription.

Example 8 PDE4D Proximal SNPs

It has been discovered that a polymorphic variation (rs1498608) in agene encoding PDE4D is associated with the occurrence of low BMD (seeExamples 1 and 2). One hundred sixteen additional allelic variantsproximal to rs1498608 were identified and subsequently allelotyped inlow BMD case and high BMD control sample sets as described in Examples 1and 2. The polymorphic variants are set forth in Table 20. Thechromosome position provided in column four of Table 20 is based onGenome “Build 34” of NCBI's GenBank. TABLE 20 Position in SEQ IDChromosome dbSNP NO: 4 Chromosome Position Alleles (A1/A2) genome_letterdeduced_iupac 6886495 249 5 58309549 g/c g S 6450498 543 5 58309843 a/ta W 1472456 973 5 58310273 g/a c Y 4700315 1076 5 58310376 a/g a R4700316 1276 5 58310576 g/c g S 7714708 1599 5 58310899 a/g a R 77104792755 5 58312055 c/t c Y 2968013 2911 5 58312211 g/c c S 2968014 4466 558313766 a/g a R 2968015 5754 5 58315054 t/c c Y 1391648 5762 5 58315062a/g c Y 2055297 5967 5 58315267 a/g c Y 2055296 5972 5 58315272 a/g t Y3989138 6390 5 58315690 —/aa a N 4700317 6984 5 58316284 c/t t Y 20362207234 5 58316534 g/a c Y 7727206 8196 5 58317496 g/t t K 7723432 8369 558317669 a/g a R 1546221 9565 5 58318865 c/t g R 4479801 11084 558320384 c/t c Y 4395595 11153 5 58320453 c/t c Y 4395596 11187 558320487 t/c c Y 4699932 11290 5 58320590 g/a a R 2936201 11386 558320686 a/g t Y 7356672 11441 5 58320741 c/t c Y 2936200 12373 558321673 a/g c Y 1909296 12602 5 58321902 c/a g K 7703131 13763 558323063 c/g c S 7445308 18697 5 58327997 a/t t W 3087748 18854 558328154 t/c g R 4321723 19107 5 58328407 c/t g R 2968016 19310 558328610 c/t c Y 5868151 20074 5 58329374 c/— c N 1874858 20145 558329445 t/c g R 1874857 20281 5 58329581 a/c t K 7712922 23117 558332417 c/t t Y 4631140 23585 5 58332885 g/a a R 4469166 23906 558333206 t/c t Y 1078369 24046 5 58333346 c/g g S 1078368 24450 558333750 g/a c Y 2968006 24619 5 58333919 a/g t Y 2968005 24637 558333937 g/t c M 2936190 24894 5 58334194 c/t c Y 2409613 25030 558334330 g/c g S 4415048 25732 5 58335032 g/c g S 2968004 27106 558336406 a/c g K 2968003 27395 5 58336695 g/a t Y 2968002 28971 558338271 a/g c Y 2936191 29755 5 58339055 t/a t W 1498610 30988 558340288 a/g g R 6874662 31827 5 58341127 c/a a M 3060393 31843 558341143 a/aca a N 7729722 32773 5 58342073 g/a a R 7733884 32787 558342087 t/c t Y 7714489 33099 5 58342399 t/c c Y 7735570 36854 558346154 t/c c Y 2936193 38026 5 58347326 g/t g K 2291851 38397 558347697 t/c c Y 2291852 38680 5 58347980 a/g a R 1498602 39626 558348926 t/c t Y 1995166 39682 5 58348982 t/c t Y 1498603 39710 558349010 g/t t K 1498604 39745 5 58349045 c/a a M 1498605 39901 558349201 g/a g R 1948651 39925 5 58349225 c/t t Y 4699934 40356 558349656 g/t t K 4700319 40393 5 58349693 c/t c Y 2279737 41230 558350530 a/g a R 7720361 41733 5 58351033 c/t c Y 7706419 41877 558351177 g/t g K 1006431 43555 5 58352855 t/g g K 1353747 44066 558353366 g/t t K 1498606 44134 5 58353434 c/t c Y 1353748 44181 558353481 t/g g K 1553113 45022 5 58354322 a/c a M 2968012 46856 558356156 t/g c M 2968011 48231 5 58357531 c/a t K 1498608 49652 558358952 a/t t W 2936189 50393 5 58359693 t/a a W 1498609 51103 558360403 c/t t Y 2968019 51733 5 58361033 t/c c Y 6891238 54733 558364033 t/c t Y 2968010 57173 5 58366473 a/t t W 2968009 58192 558367492 c/t g R 2936203 58506 5 58367806 g/c g S 1498601 59572 558368872 g/a t Y 1498600 59738 5 58369038 a/g t Y 1498599 61617 558370917 g/a t Y 2936202 63980 5 58373280 c/g c S 7730070 64161 558373461 c/g g S 6450501 66871 5 58376171 a/g g R 6450502 67063 558376363 a/t a W 6889456 67084 5 58376384 a/g a R 6894618 67477 558376777 c/t t Y 7706044 69282 5 58378582 t/c t Y 7707541 70363 558379663 a/t a W 7712076 70647 5 58379947 c/t t Y 6892860 71834 558381134 c/t c Y 6867053 72130 5 58381430 c/g c S 7737269 73495 558382795 c/t t Y 6864156 74542 5 58383842 t/c t Y 950447 75280 558384580 t/c a R 2936196 80740 5 58390040 a/g g R 7719347 82579 558391879 t/c t Y 1391649 82591 5 58391891 c/a a M 1391650 82976 558392276 t/c c Y 1391651 83040 5 58392340 g/a a R 1353749 85894 558395194 g/a a R 10682149 86020 5 58395320 g/gcct g N 5868153 86947 558396247 g/ag g N 1363882 88922 5 58398222 c/g g S 2409626 89662 558398962 t/c t Y 2968018 92367 5 58401667 g/a c Y 954740 93154 558402454 a/g c Y 986067 94979 5 58404279 t/c c Y 6869400 97598 558406898 t/c c Y 5010782 98532 5 58407832 t/a a W

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype the proximal SNPs of Table 20are the same methods described in Examples 1 and 2 herein. The primersand probes used in these assays are provided in Table 21 and Table 22,respectively. TABLE 21 DbSNP rs# Forward PCR primer Reverse PCR primer6886495 AGTTTGCTTCCTGAACAATC ATGCAGTTGAATCTCAATAC 6450498GGACAGCTTTATGTTTAATAC ACAAATACTCCATTGATGGT 1472456 GGGAGGTAAGGAATCATGACTTTTGAAGGACAAGTTCCCC 4700315 GGGAACTTGTCCTTCAAAAAG CATTTTCTGTTACTCTGAGG4700316 ATTGTGTCCATGCTTGGCAG CCTCCTTCTATTGTGGAGAC 7714708GCAACATACTAACTGGAATCC GTTCTTCTGTTTGTCATGTGG 7710479 TGCTCCAAAAATGGCAATCGATTTCTACATCACCAGGGAG 2968013 CACCCCTAGATTTTGAAGGT CAATGACACATTCCCTCACC2968014 CCTTCCCTCCTTCATTCAAC TGGATTCAGATTACGGGAGG 2968015TGTCAGCTGTATGGTGAAGG AACGCCAAGCCTATTTTCAG 1391648 AATTTGGTAAGACAGAGATGTTGCTCTGTTCCTGAAAGAC 2055297 AAGCTTTGTTTCTCTTTCTC AACGATTCTACATCTGCCCC2055296 AAGCTTTGTTTCTCTTTCTC AACGATTCTACATCTGCCCC 3989138GCCTTCCCCCTCCTTTAAAA CATTTCAGGATGAATACTTG 4700317 TTTTCCTGATATGATGAATGCTAGTAATGCAGCAATAGTG 2036220 TGTATCCCAGGACATCTAGC AACATTCAGGAAATTTCAGG7727206 AAGGACTGGGTTTGCATTTC CTAATCTTGGCAACATTCTG 7723432TGAATCTAGGAGTGGATTTG AGATCTGACCATGGTGATAG 1546221 GGAAAACTCCTTATGTTGGAGCTGTATATTATATTACTGTG 4479801 ACTTCTAGAAGAAAACACAG TATGCTTCATGCTTTTGTGG4395595 AAACCACAAAAGCATGAAGC TGGCTTGTTGTTCTGTTTTC 4395596ATATTCCCTTCTCAAGTCTG CTGGACTTTACCAAATTTCTC 4699932 CCATCTTTTCACTGGATTGTCCAGACTTGAGAAGGGAATATG 2936201 GCATCTCACTGTGGTTTTAC CACAATAAGAGGACAATCCAG7356672 GACCATCATTAGACATTAGGG TTAATTTGCTCTGCATCCAG 2936200TCACAACTGGGAATTCACTG GAGCTCCACCATTATTTCCC 1909296 CCGAGTAGCTGGGATTACATTGGTGAAACCCTGTCTCTAC 7703131 AGAGAAGAAGGCTTCAGACG TTTAGCTTCTTTGCGATGGG7445308 ATACAGCGGTTGGGACTATC GCACATTGTGCACATGTACC 3087748TAGTCCATAGGAATCTGCTG GCTGCTGTACATTACAACAC 4321723 GAATTTTACTTGGAACCCTGGCCTATCTTACTACTGAACTC 2968016 GTAAACCTTGGACTTATGGG ACGATGTTACCCTCTTTTCC5868151 TAGAATAGACTACATCCATC GGATAAGGAAGTTTCTTAGG 1874858GCCTAAGAAACTTCCTTATCC ATATTGCCAACTAGGAGTAC 1874857 ATGAAGACTTTACTGAAGGCCATACACTACTAACCTGTTGC 7712922 CTCTTTCTAAGGGCTTCTGG AGAGGAGTCGGACTTTGTTC4631140 TTGAAGTTGAGAGGGTCTCC AAGCAAAGCACAAGCAACAG 4469166TTAGGAGGGATGAGGAATGG CTCCAATTGCACTGGGTTAC 1078369 TCCCTGAGCCTCTGTTTTCCATATGTCCCCACCACACTAC 1078368 ATGACTCATGGAGGCAACAG GGAGAGCGATTTATGGATGG2968006 CAGGGTTCATTTGGTGAAAC ACTGACCTGTCTGGGATTTC 2968005ACTGACCTGTCTGGGATTTC CGCAGGGTTCATTTGGTGAA 2936190 AACAGACCTCCAACACAACCTAGGATAAACTCACGGAAGG 2409613 AATAGGGTCAGTGGGATGAG CCCACCTCCAAATCCTTTAC4415048 CACCCAGCCTCAGAATATTT TTCCCCACCAATACAACATC 2968004CCCAAACATTATCTTCTGGC GATCTCCATAAGGGTAAGTG 2968003 ACAAGAGGACAAGTGTTTAGGGCCAGAGCTGTTCATAAAT 2968002 CCTTTGACTTTCCACAGAAC ACACTCACTGGGTTGGGCTA2936191 TGACCTTGATAACCTGGCTG AGCTGTGCATATTGACTTCC 1498610CATTAAATCACCACAGCAAC TCCTTAGGCAGAATGGTC 6874662 ACTCTAGCCTGGGCAACAGAATGTCTTGTAGTTTCCAGTG 3060393 CCAGTGTATAGATCTTTCACC AGAGCGAGACTCCATCACAA7729722 GAGGAAAGTGTGTCTATTCAG CCATACATCTGATAAGAGGC 7733884TGTATCTCTGTTGGCCATTC CCATACATCTGATAAGAGGC 7714489 AGGGTCTTGCTCTTTTGTTCATTGAGCCCGAGAATTTGAG 7735570 AGGATCTTCATAGAAGTGGC AACTGAGGCTGTTTTCTCTC2936193 CTTAGCATACAATGGGCACC ACAGTGCCTACTATTGTCAG 2291851GTTTCTTGCTTGTTGATGGC TAGCCTTGGCCAAGAATTCA 2291852 ATCCTGATCTCTTACTAGTGTTCTGTGTTCTCTCTTGAGG 1498602 CAGTAGTTTTTCAGCTAAATG ATCCAAACATAGATCTCAAC1995166 GCCACTTAGCAATGTGCAAG GGGAAAGTAACTAGCTGCTC 1498603GTGTGGTTGATTCTGTGTAAG ACTACTGTCGAATCACCTTG 1498604 GCTTGCACATTGCTAAGTGGCCATGGGCCTGAGTTCTTAA 1498605 CTGTGGCATTAGGCACCTTT AGGACACTGGACATATTCAG1948651 CAGATGAGAATAAAGGTGCC TATTGCTCTCTTACTGGGAC 4699934AGGACTGGTAATGTTGTGAG GACCTTGTAAATAGGTGGCC 4700319 GACCTTGTAAATAGGTGGCCGCTGAAGGATTCAGCCAGTA 2279737 AAAACCTCTCAATTTATTTC ATGGCCTCCAACAAGGTAAG7720361 ACTATGCGACTTTTCAACTG ACCCCCTCGCCATCCGCTA 7706419ACCCGGCCGCGGCTGATTCAT GCCCCGCCTGCCGAGCCTT 1006431 CCTTGCCAGGTGAATTAAAGCTATACAGAGCAGGTATTTT 1353747 TGAGAAAGTTGGAGTGCAGG AATCATTGGTTACAATGAAG1498606 ACTCCAACTTTCTCAAAGCC TTGGTGAAGATGGAGGAAGG 1353748CTCCATCTTCACCAAGTTCC GAAATATAATGTGTGGAGCC 1553113 AGCCTTTAGGGAGTTTAGCCGCACCAAATCTTGCTAAGTC 2968012 CAGTTCTATCTACTGTAGAC CAGTTCCAGCTTCTCTTCTC2968011 ATAATGGTTGCACTGACTTC ACTGTGTGACATGGGATCTG 1498608GAATCCCTGTTCATTCCTTG ATAACCTCGGGGTCCAGAAA 2936189 TGATCTCAGACTTCCAGCTCTTTGTCTTAGCTCAAGCTGC 1498609 TAGCTCAACTGTTCTTCAGG CAGAGTGAGTGTAAATATAC2968019 CCCAGGCCAGTATTACTGTT TGACATTTACAGGCACTCAG 6891238CCTTTATTCAGGCTGCAGAC TGTGGTTTTAATGGCTGTGG 2968010 ATGTATGATTCACTCTGATGCAAACCAATTGGTAGATTTC 2968009 GTAGGCAAAGATGAATCACG TGTTTTACCTAGCAAGGAGC2936203 AGTTCAGAGGTTCCAGACAG AGCCAAGCTTGCAAACTCTG 1498601TTGGTTAGATCCAGCTCTGC TAAGAGGAACAGGGATCTGC 1498600 ATATAGGTACTGCTTTCTCCCCTTGTTTCCAAATCTGAGC 1498599 GGAACATTTGGCTACATCATG CCACAGAGCTGATTTAATTTC2936202 TGTAAGAGGAGGGTGTGATG TGACTCTGCAGGACTGTCTG 7730070AAATATACTTGGGTAGAGAG GATAGCTAACACATTTCTGAC 6450501 AGCTAGACTACATAGCCTCCTGTAGGACATGACAGCAAAC 6450502 TAACAAGTCAAAACAGATGG ATTTTCAATATTCTGCACAC6889456 GGATCTCTATTAAACCTCTC TTCAAGGGTTACTGATACTC 6894618CCATCAACAGATGAATGGAC TCCTTCCTTTTTTAGGGCTG 7706044 GACTTGGTATTTTGTGAGGGTCCACCATGTGAGGACAAAG 7707541 TTTTGAGTCATGAAAAACTG CACATGTATTAATTAAGTAGG7712076 AAGCCATACCACAAGCATTC GTTCACGTGAAATACACTACC 6892860CAGAAGATGCAATGAAAAGAC AGAACAAAATGTTAGGACGC 6867053 TTATCCGCATTCCACTCTTGACACTGGTCCTCATAACAAG 7737269 TTTAAAGGCTCGCACCTCAG TCCGAAGGAAAGTGATTCTC6864156 GTTGGATCATCAAAAAGTGG GGCTAGAGTACTAAAAATCAG 950447GTGAGTAGTCTGAATTTGTC AGGCTTCAGAATCGGTCATC 2936196 CTCCAAAACATAGTAAGTGCTGAACATTTAGAATTAGGGG 7719347 ACATTCTGAGGTCATGCAGG CTCAGAGCCTGCATTATCTC1391649 GCTAATGCTTTACTCTGGTC CACACTCCAAAATGTGCAAC 1391650TTCAGCTGATACTTGCTCCC CTCTACCTACCAACATCGTC 1391651 TTGACGTGATAGAAGTTTGGCTGAAAAGGGTGGCTTTCTC 1353749 TATAGAGCAAAAAGCCAAGG CCACTTCTACCACATTCTTC10682149 GGGAGAATATAACCATTAAGT GAATTGCATTTTATCCAATC 5868153CATAGGGACTATTTAAACTTC GAGTGTTCTTTAGAGATTAAG 1363882 CTCACACAGGCAATGAGTAGAGCCACTACTTCTCAATCTC 2409626 CCTGGTCTCAAGCAATCATC ATAAGGCCAGATGTGGTGAC2968018 GCAGAGAGATGAGAGGAAAC CCTCATATCTAATCTCTCCC 954740ATACTTGGGAGCACTCAACG CAACAAGACGGAATCCAAAG 986067 CTACAAATTGCTTAAGCAGGGTGATAGAGTAGAGAGACTCC 6869400 ACAAGATCGTTGAATGGTGG GACTTTGTATACTGCCACTC5010782 AATCTGTGGGAGTTAGTGGT TTCCTCATCCTCATCTTCCC

TABLE 22 dbSNP Extend Term rs# Primer Mix 6886495 CCTGAACAATCTTAAATGCACT 6450498 GTCTTATGCATTTTGAAGG CGT 1472456 GGAATCATGACTACTTGGA ACG4700315 GAAACAAAGCAAACGAC ACT 4700316 TGCTTGGCAGGCTTTTT ACT 7714708CAGTATAAGTAATTTGGCCC ACT 7710479 GAGCATTTTAATTGCTTCC ACG 2968013AGCTGAGAGCAGCCATG ACT 2968014 GAGCTGTTTCTTTCAGTTT ACT 2968015ATGGTGAAGGTGGAGGA ACT 1391648 TCAGCTGTATGGTGAAG ACT 2055297GTGCCTGACATAGAGTAG ACT 2055296 GGCAGTGCCTGACATAG ACT 3989138CCCCTCCTTTAAAAAAAAAAA CGT 4700317 CAAATGTAACAAAGTGCCA ACG 2036220GCCTCCCTGGCCTCTGC ACG 7727206 AATCCTGTCACCTATGG CGT 7723432GTGCCTTGAATTAAAGAATC ACT 1546221 CTTATGTTGGAGAGGTC ACG 4479801TAGAAAGTAGTTGTGATCTTG ACG 4395595 AAAAATGGTATACTGGACTTTA ACG 4395596CTGTGGCTTGTTGTTCT ACT 4699932 CACTGGATTGTCCTCTTA ACG 2936201CCTAATGTCTAATGATGGTC ACT 7356672 CACAGTGAGATGCCACA ACG 2936200CTTGGATAACTGTATGCCT ACT 1909296 TTACATGAGTGCACCAC CGT 7703131GGTAATAAACTTTTCCGAG ACT 7445308 GTTGGGACTATCTACTTTTTT CGT 3087748CATAGGAATCTGCTGCCTCAC ACT 4321723 ACTTGGAACCCTGGTATTTT ACG 2968016GGGGCAAAAAGACAATAAA ACG 5868151 GAATAGACTACATCCATCAAAATA ACG 1874858GAAACTTCCTTATCCAAGGCC ACT 1874857 GCAATTAAAAGTGGTACAATAA ACT 7712922CAATAAAAGGAAAGAGACTTC ACG 4631140 AGAGGGTCTCCTGTAGG ACG 4469166GAGGAATGGAACTATTTTTAAAC ACT 1078369 CTCTGTTTTCCCTGTAAAT ACT 1078368GCAACAGGTGAAGCTGA ACG 2968006 GACTGAACCTTTAGGAGA ACT 2968005TTCTCCTAAAGGTTCAGT CGT 2936190 CCAACACAACCTCTTAA ACG 2409613CCAAGTGACTAAAGCAGAT ACT 4415048 AAAACCTAATGCTCAGTTAAA ACT 2968004TCTGGCATAAAGTAAACTAATC ACT 2968003 GGGGACTAGTCAACAAA ACG 2968002TCTGATGAGGTCTTCTAC ACT 2936191 GGCTGAAGTAGTGATAGG CGT 1498610GAAGCACAGAAGGCAAC ACT 6874662 CAGAGCGAGACTCCATC CGT 3060393GATTTGCTCCTAAGTTTTTT ACT 7729722 GTGTCTATTCAGATCCTTTG ACG 7733884TTGAGGAAAGTGTGTCT ACT 7714489 GGCTGGAGTGCAGTGGC ACT 7735570TCCACATTATGCAACTACA ACT 2936193 AAAAGTCAGTGTAAGAGTTC CGT 2291851TGATGGCATTGAAGCAG ACT 2291852 GTTTTTATTGTGCTTTTCAATCT ACT 1498602GAATTTGAGCAGCTAGTT ACT 1995166 TGTGCAAGCATTAGTGTAT ACT 1498603GAGCAGAATCAAAAGCC CGT 1498604 GCTCTTCATTTTCTTACACAG CGT 1498605AGGCACCTTTATTCTCAT ACG 1948651 GAATAAAGGTGCCTAATGC ACG 4699934GTAATAGTTGCCTCGTC CGT 4700319 TTACTAATTGTCTCACAACATTA ACG 2279737TCGCCGGCATGGGAATC ACT 7720361 AGTCCCAGTCCAGGAGC ACG 7706419TTCATTCACTTCAAGTGC CGT 1008431 GTGAATTAAAGTTTCAGATTGAA ACT 1353747GGAGAGGAGCCACAGAA CGT 1498606 CAAGTGATTTCCCAGTAG ACG 1353748ACCAAGTTCCCAAAGCT ACT 1553113 GGAGTTTAGCCAATAGTTTTTGC ACT 2968012GACAGTATCAATTCCTTCC ACT 2968011 TGACTTCAACTTTACTTTCTT CGT 1498608CCCTAAAAACTGTTCCAGGTA CGT 2936189 CCAGAACTGTGAGAAATAAA CGT 1498609GTTTTCTATTTGAAATTGAGGTAT ACG 2968019 CCAGTATTACTGTTTGAATCTC ACT 6891238CTGCAGACATTTCCTAC ACT 2968010 TTCACTCTGATGTTTTCTATTT CGT 2968009CATAACCTCTGGTTTCC ACG 2936203 GGAGGATATCCATGCCCC ACT 1498601AGCTCTGCCTCCCCCATTT ACG 1498600 AGGTGCCAAGCTAGTCA ACT 1498599GGCTACATCATGTTTTGG ACG 2936202 CCCTGGTAACTGTAGTG ACT 7730070TAGGCATGCTCAAACTC ACT 6450501 GCCTCCTAAAAAAGCAC ACT 6450502GTTTAATGACATTTTAGAGAGG CGT 6889456 AACCTCTCTAAAATGTCATTAA ACT 6894618GGACAAAGGAAATGTGATATATA ACG 7706044 GGCTCATTTCCTGGGTC ACT 7707541GAAAAACTGACAACATATGAG CGT 7712076 CCCAGAGGATATAGATTTCAT ACG 6892860GGATATTTCAGTGGTGCT ACG 6867053 CACCATTAACCTGGTCTA ACT 7737269ACTCTTGATCAATAGTTTGG ACG 6864156 GTAGTTGACAAATCTTACCT ACT 950447TTGTTAGTGTATTAGCCATTG ACT 2936196 GTGCAAAGATCAATCACA ACT 7719347GGTCATGCAGGAAATAG ACT 1391649 TCTGGTCTATTTCCTGC CGT 1391650CCCACAGGTTTTTCACA ACT 1391651 GGGCCCCCGTTTTGCCCTG ACG 1353749CAAAAAGCCAAGGATATAAAATA ACG 10682149 GTATGATCTGATTTCCATAAATAG ACT5868153 GAAATGAATAAATGGCAAAAAA ACT 1363882 GCAATGAGTAGACTAAAAAAA ACT2409626 AGCAATCATCCCACCTC ACT 2968018 CAGACACCCACTGACCA ACG 954740ACGAAGGTCCCGTCTGA ACT 986067 CAGGGATTTAAAATGAAACC ACT 6869400AATGGTGGGTCATTAGTT ACT 5010782 GCACCTACAGAGGAAAT CGT

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table23. The allele frequency for the A2 allele is noted in the fifth andsixth columns for control pools and case pools, respectively, where “AF”is allele frequency. Some SNPs do not have an allele frequency disclosedbecause of failed assays. TABLE 23 Position in Low BMD dbSNP SEQ IDChromosome A1/A2 Control AF Case AF Associated rs# NO: 4 Position Allele(High BMD) (Low BMD) p-Value OR Allele 6886495 249 58309549 G/C G = G =0.87 C = C = 0.13 6450498 543 58309843 A/T A = 0.81 A = 0.85 0.1780 1.29A T = 0.19 T = 0.15 1472456 973 58310273 G/A G = 1.00 G = 1.00 A = 0.00A = 0.00 4700315 1076 58310376 A/G A = A = 0.95 G = G = 0.05 47003161276 58310576 G/C G = 0.87 G = 0.84 0.1520 0.75 C C = 0.13 C = 0.167714708 1599 58310899 A/G A = 0.35 A = 0.46 0.0013 1.60 A G = 0.65 G =0.54 7710479 2755 58312055 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.002968013 2911 58312211 G/C G = 0.84 G = 0.80 0.1256 0.79 C C = 0.16 C =0.20 2968014 4466 58313766 A/G A = 0.22 A = 0.25 0.2834 1.16 A G = 0.78G = 0.75 2968015 5754 58315054 T/C T = 0.88 T = 0.86 0.2038 0.80 C C =0.12 C = 0.14 1391648 5762 58315062 A/G A = 0.25 A = 0.24 0.8566 0.97 GG = 0.75 G = 0.76 2055297 5967 58315267 A/G A = 0.00 A = 0.00 G = 1.00 G= 1.00 2055296 5972 58315272 A/G A = 0.94 A = 0.94 0.8857 1.04 A G =0.06 G = 0.06 3989138 6390 58315690 —/AA — = 0.53 — = 0.50 0.3703 0.90 AAA = 0.47 AA = 0.50 4700317 6984 58316284 C/T C = 0.00 C = 0.00 T = 1.00T = 1.00 2036220 7234 58316534 G/A G = 0.44 G = 0.44 0.9272 1.01 G A =0.56 A = 0.56 7727206 8196 58317496 G/T G = 0.27 G = 0.25 0.3954 0.89 TT = 0.73 T = 0.75 7723432 8369 58317669 A/G A = 0.82 A = 0.82 0.76711.05 A G = 0.18 G = 0.18 1546221 9565 58318865 C/T C = 0.83 C = 0.840.7282 1.06 C T = 0.17 T = 0.16 4479801 11084 58320384 C/T C = 0.86 C =0.87 0.7440 1.07 C T = 0.14 T = 0.13 4395595 11153 58320453 C/T C = 0.93C = 0.93 0.7241 1.09 C T = 0.07 T = 0.07 4395596 11187 58320487 T/C T =0.01 T = 0.02 C = 0.99 C = 0.98 4699932 11290 58320590 G/A G = 0.13 G =0.10 0.1440 0.76 A A = 0.87 A = 0.90 2936201 11386 58320686 A/G A = 0.17A = 0.19 0.3609 1.15 A G = 0.83 G = 0.81 7356672 11441 58320741 C/T C =0.88 C = 0.89 0.4757 1.17 C T = 0.12 T = 0.11 2936200 12373 58321673 A/GA = 1.00 A = 1.00 G = 0.00 G = 0.00 1909296 12602 58321902 C/A C = 0.98C = 0.99 A = 0.02 A = 0.01 7703131 13763 58323063 C/G C = C = G = G =7445308 18697 58327997 A/T A = 0.00 A = 0.00 T = 1.00 T = 1.00 308774818854 58328154 T/C T = T = 0.21 C = C = 0.79 4321723 19107 58328407 C/TC = 0.19 C = 0.24 0.1033 1.30 C T = 0.81 T = 0.76 2968016 19310 58328610C/T C = 0.45 C = 0.44 0.7959 0.96 T T = 0.55 T = 0.56 5868151 2007458329374 C/— C = 0.44 C = 0.43 0.6659 0.95 — — = 0.56 — = 0.57 187485820145 58329445 T/C T = 0.12 T = 0.17 0.1558 1.43 T C = 0.88 C = 0.831874857 20281 58329581 A/C A = 0.96 A = 0.96 0.5485 0.83 A C = 0.04 C =0.04 7712922 23117 58332417 C/T C = 0.05 C = 0.00 T = 0.95 T = 1.004631140 23585 58332885 G/A G = 0.10 G = 0.09 0.4903 0.87 A A = 0.90 A =0.91 4469166 23906 58333206 T/C T = 0.97 T = 0.98 0.6510 1.30 T C = 0.03C = 0.02 1078369 24046 58333346 C/G C = 0.88 C = 0.88 0.8279 0.96 C G =0.12 G = 0.12 1078368 24450 58333750 G/A G = 0.50 G = 0.47 0.3033 0.89 AA = 0.50 A = 0.53 2968006 24619 58333919 A/G A = 0.34 A = 0.35 0.52161.08 A G = 0.66 G = 0.65 2968005 24637 58333937 G/T G = 0.37 G = 0.360.7332 0.96 T T = 0.63 T = 0.64 2936190 24894 58334194 C/T C = 0.22 C =0.22 0.9071 1.02 C T = 0.78 T = 0.78 2409613 25030 58334330 G/C G = 1.00G = 1.00 C = 0.00 C = 0.00 4415048 25732 58335032 G/C G = 0.94 G = 0.960.2666 1.36 G C = 0.06 C = 0.04 2968004 27106 58336406 A/C A = 0.92 A =0.88 0.1210 0.70 C C = 0.08 C = 0.12 2968003 27395 58336695 G/A G = 0.61G = 0.66 0.2364 1.23 G A = 0.39 A = 0.34 2968002 28971 58338271 A/G A =0.81 A = 0.81 0.8196 1.04 A G = 0.19 G = 0.19 2936191 29755 58339055 T/AT = 1.00 T = 1.00 A = 0.00 A = 0.00 1498610 30988 58340288 A/G A = 0.00A = 0.00 G = 1.00 G = 1.00 6874662 31827 58341127 C/A C = 0.00 C = 0.00A = 1.00 A = 1.00 3060393 31843 58341143 —/CA — = 1.00 — = 1.00 CA =0.00 CA = 0.00 7729722 32773 58342073 G/A G = 0.01 G = 0.01 A = 0.99 A =0.99 7733884 32787 58342087 T/C T = 0.96 T = 0.97 0.5282 1.26 T C = 0.04C = 0.03 7714489 33099 58342399 T/C T = 0.15 T = 0.14 0.7668 0.95 C C =0.85 C = 0.86 7735570 36854 58346154 T/C T = 0.08 T = 0.09 0.4593 1.18 TC = 0.92 C = 0.91 2936193 38026 58347326 G/T G = 0.25 G = 0.28 0.36381.14 G T = 0.75 T = 0.72 2291851 38397 58347697 T/C T = 0.05 T = 0.060.2114 1.40 T C = 0.95 C = 0.94 2291852 38680 58347980 A/G A = 0.98 A =0.98 G = 0.02 G = 0.02 1498602 39626 58348926 T/C T = 0.67 T = 0.610.0496 0.77 C C = 0.33 C = 0.39 1995166 39682 58348982 T/C T = 0.54 T =0.55 0.7840 1.03 T C = 0.46 C = 0.45 1498603 39710 58349010 G/T G = 0.03G = 0.02 T = 0.97 T = 0.98 1498604 39745 58349045 C/A C = 0.16 C = 0.130.2329 0.79 A A = 0.84 A = 0.87 1498605 39901 58349201 G/A G = 0.88 G =0.89 0.3713 1.18 G A = 0.12 A = 0.11 1948651 39925 58349225 C/T C = 0.58C = 0.56 0.5199 0.93 T T = 0.42 T = 0.44 4699934 40356 58349656 G/T G =0.13 G = 0.05 0.0043 0.35 T T = 0.87 T = 0.95 4700319 40393 58349693 C/TC = C = 0.98 T = T = 0.02 2279737 41230 58350530 A/G A = 0.82 A = 0.800.3520 0.87 G G = 0.18 G = 0.20 7720361 41733 58351033 C/T C = 1.00 C =1.00 T = 0.00 T = 0.00 7706419 41877 58351177 G/T G = 0.89 G = 0.920.1509 1.38 G T = 0.11 T = 0.08 1006431 43555 58352855 T/G T = 0.22 T =0.30 0.0077 1.49 T G = 0.78 G = 0.70 1353747 44066 58353366 G/T G = 0.23G = 0.15 0.0025 0.61 T T = 0.77 T = 0.85 1498606 44134 58353434 C/T C =0.85 C = 0.87 0.3475 1.17 C T = 0.15 T = 0.13 1353748 44181 58353481 T/GT = 0.65 T = 0.66 0.8645 1.02 T G = 0.35 G = 0.34 1553113 45022 58354322A/C A = 0.95 A = 0.97 0.1730 1.54 A C = 0.05 C = 0.03 2968012 4685658356156 T/G T = 0.66 T = 0.66 0.9069 1.01 T G = 0.34 G = 0.34 296801148231 58357531 C/A C = 0.67 C = A = 0.33 A = 1498608 49652 58358952 A/TA = 0.19 A = 0.13 0.0118 0.64 T T = 0.81 T = 0.87 2936189 50393 58359693T/A T = 0.63 T = 0.65 0.6021 1.08 T A = 0.37 A = 0.35 1498609 5110358360403 C/T C = 0.34 C = 0.40 0.0314 1.30 C T = 0.66 T = 0.60 296801951733 58361033 T/C T = 0.62 T = 0.64 0.5417 1.08 T C = 0.38 C = 0.366891238 54733 58364033 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 296801057173 58366473 A/T A = 0.90 A = 0.95 0.0012 2.28 A T = 0.10 T = 0.052968009 58192 58367492 C/T C = 0.65 C = 0.70 0.0959 1.24 C T = 0.35 T =0.30 2936203 58506 58367806 G/C G = 0.17 G = 0.14 0.1730 0.80 C C = 0.83C = 0.86 1498601 59572 58368872 G/A G = 0.81 G = 0.78 0.3899 0.83 A A =0.19 A = 0.22 1498600 59738 58369038 A/G A = 0.48 A = 0.46 0.5991 0.94 GG = 0.52 G = 0.54 1498599 61617 58370917 G/A G = 0.69 G = 0.69 0.97391.00 G A = 0.31 A = 0.31 2936202 63980 58373280 C/G C = 0.20 C = 0.140.0286 0.68 G G = 0.80 G = 0.86 7730070 64161 58373461 C/G C = 0.74 C =0.73 0.5744 0.93 G G = 0.26 G = 0.27 6450501 66871 58376171 A/G A = 0.00A = 0.09 G = 1.00 G = 0.91 6450502 67063 58376363 A/T A = 1.00 A = 1.00T = 0.00 T = 0.00 6889456 67084 58376384 A/G A = A = 1.00 G = G = 0.006894618 67477 58376777 C/T C = 0.69 C = 0.73 0.1542 1.21 C T = 0.31 T =0.27 7706044 69282 58378582 T/C T = T = 1.00 C = C = 0.00 7707541 7036358379663 A/T A = 0.63 A = 0.61 0.5874 0.93 T T = 0.37 T = 0.39 771207670647 58379947 C/T C = 0.00 C = 0.00 T = 1.00 T = 1.00 6892860 7183458381134 C/T C = 0.81 C = 0.83 0.4091 1.14 C T = 0.19 T = 0.17 686705372130 58381430 C/G C = 0.46 C = 0.44 0.4563 0.91 G G = 0.54 G = 0.567737269 73495 58382795 C/T C = 0.00 C = 0.00 T = 1.00 T = 1.00 686415674542 58383842 T/C T = 0.99 T = 0.99 C = 0.01 C = 0.01 950447 7528058384580 T/C T = T = 1.00 C = C = 0.00 2936196 80740 58390040 A/G A =0.00 A = 0.00 G = 1.00 G = 1.00 7719347 82579 58391879 T/C T = 1.00 T =1.00 C = 0.00 C = 0.00 1391649 82591 58391891 C/A C = 0.42 C = 0.350.0162 0.74 A A = 0.58 A = 0.65 1391650 82976 58392276 T/C T = 0.75 T =0.75 0.9568 0.99 T C = 0.25 C = 0.25 1391651 83040 58392340 G/A G = 0.58G = 0.59 0.8831 1.02 G A = 0.42 A = 0.41 1353749 85894 58395194 G/A G =0.43 G = 0.39 0.1596 0.85 A A = 0.57 A = 0.61 10682149 86020 58395320—/CCT — = 0.37 — = 0.39 0.5382 1.08 — CCT = 0.63 CCT = 0.61 586815386947 58396247 A/— A = 0.72 A = 0.71 0.7470 0.96 — — = 0.28 — = 0.291363882 88922 58398222 C/G C = 0.45 C = 0.43 0.5228 0.93 G G = 0.55 G =0.57 2409626 89662 58398962 T/C T = 0.84 T = 0.85 0.8581 1.03 T C = 0.16C = 0.15 2968018 92367 58401667 G/A G = 0.75 G = 0.75 0.8411 0.97 G A =0.25 A = 0.25 954740 93154 58402454 A/G A = 0.00 A = 0.00 G = 1.00 G =1.00 986067 94979 58404279 T/C T = 0.04 T = 0.06 0.2475 1.43 T C = 0.96C = 0.94 6869400 97598 58406898 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.005010782 98532 58407832 T/A T = 0.68 T = 0.69 0.8644 1.02 T A = 0.32 A =0.31

Allelotyping results were considered particularly significant with acalculated p-value of less than or equal to 0.05 for allelotype results.These values are indicated in bold. The allelotyping p-values wereplotted in FIG. 4. The position of each SNP on the chromosome ispresented on the x-axis. The y-axis gives the negative logarithm (base10) of the p-value comparing the estimated allele in the case group tothat of the control group. The minor allele frequency of the controlgroup for each SNP designated by an X or other symbol on the graphs inFIG. 4 can be determined by consulting Table 23. For example, theleft-most X on the left graph is at position 58309549. By proceedingdown the Table from top to bottom and across the graphs from left toright the allele frequency associated with each symbol shown can bedetermined.

To aid the interpretation, multiple lines have been added to the graph.The broken horizontal lines are drawn at two common significance levels,0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assistin the interpretation of distances between SNPs. Two other lines aredrawn to expose linear trends in the association of SNPs to the disease.The light gray line (or generally bottom-most curve) is a nonlinearsmoother through the data points on the graph using a local polynomialregression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992)Local regression models. Chapter 8 of Statistical Models in S eds J. M.Chambers and T. J. Hastie, Wadsworth & Brooks/Cole.). The black lineprovides a local test for excess statistical significance to identifyregions of association. This was created by use of a 10 kb slidingwindow with 1 kb step sizes. Within each window, a chi-square goodnessof fit test was applied to compare the proportion of SNPs that weresignificant at a test wise level of 0.01, to the proportion that wouldbe expected by chance alone (0.05 for the methods used here). Resultingp-values that were less than 10⁻⁸ were truncated at that value.

Finally, the exons and introns of the genes in the covered region areplotted below each graph at the appropriate chromosomal positions. Thegene boundary is indicated by the broken horizontal line. The exonpositions are shown as thick, unbroken bars. An arrow is place at the 3′end of each gene to show the direction of transcription.

Example 9 GPX3 Proximal SNPs

It has been discovered that a polymorphic variation (rs869975) in a geneencoding GPX3 is associated with the occurrence of low BMD (see Examples1 and 2). Two hundred thirty-three additional allelic variants proximalto rs869975 were identified and subsequently allelotyped in low BMD caseand high BMD control sample sets as described in Examples 1 and 2. Thepolymorphic variants are set forth in Table 24. The chromosome positionprovided in column four of Table 24 is based on Genome “Build 34” ofNCBI's GenBank. TABLE 24 Position in SEQ ID Chromosome dbSNP NO: 5Chromosome Position Alleles (A1/A2) genome_letter deduced_iupac 1478398231 5 150385031 a/g t Y 1478397 330 5 150385130 t/c a R 1160114 582 5150385382 g/c g S 1160113 589 5 150385389 a/g c Y 1382323 1060 5150385860 a/g c Y 1160112 1066 5 150385866 g/a t Y 7709870 1311 5150386111 g/a a R 7710643 1556 5 150386356 g/t g K 7730467 1655 5150386455 t/c t Y 6579829 1692 5 150386492 a/c a M 6579830 1802 5150386602 g/a g R 6579831 2061 5 150386861 t/a a W 6896232 2112 5150386912 t/a t W 1351131 2153 5 150386953 t/c g R 1038074 2667 5150387467 c/t g R 1478396 3115 5 150387915 t/c a R 6880512 3186 5150387986 g/a a R 4958858 5621 5 150390421 t/c t Y 4958431 5735 5150390535 t/g g K 4958432 5829 5 150390629 g/c g S 6898463 6658 5150391458 a/c c M 4958859 7901 5 150392701 g/c c S 4130064 11447 5150396247 g/a g R 4130065 11466 5 150396266 a/g a R 4133119 11984 5150396784 t/c g R 4958860 15803 5 150400603 t/g t K 4958861 16257 5150401057 t/g t K 4437356 17604 5 150402404 c/a c M 4958868 19762 5150404562 c/t t Y 1478400 22367 5 150407167 a/g g R 6889375 22709 5150407509 a/g g R 1600159 23631 5 150408431 g/c c S 6875892 23686 5150408486 t/a t W 4608909 25599 5 150410399 t/c t Y 2345000 26973 5150411773 a/c c M 4516840 28457 5 150413257 g/t t K 2054440 28669 5150413469 a/g a R 707141 29908 5 150414708 c/t a R 707142 30105 5150414905 a/g t Y 841236 30711 5 150415511 a/g a R 707143 30851 5150415651 g/a t Y 707144 31203 5 150416003 t/c g R 6869405 31446 5150416246 a/c c M 707145 31638 5 150416438 c/t g R 707146 33064 5150417864 c/t g R 707148 33958 5 150418758 c/a t K 707150 35182 5150419982 a/t t W 5872184 38332 5 150423132 a/ac a N 3763015 40875 5150425675 g/a c Y 2042235 41624 5 150426424 t/c t Y 3763013 41671 5150426471 a/g c Y 2042236 41825 5 150426625 g/a g R 1946234 42920 5150427720 c/a a M 1946235 42935 5 150427735 t/c t Y 1946236 43001 5150427801 t/a a W 8177402 43012 5 150427812 t/c c Y 8177403 43203 5150428003 c/t c Y 8177404 43294 5 150428094 t/c t Y 8177405 43295 5150428095 t/c c Y 8177406 43344 5 150428144 c/t t Y 8177407 43509 5150428309 t/c c Y 8177408 43549 5 150428349 g/c c S 8177409 43560 5150428360 t/a a W 6888961 43578 5 150428378 a/t t W 8177410 43640 5150428440 a/g g R 8177411 43792 5 150428592 g/c g S 8177412 43797 5150428597 c/t t Y 8177413 43964 5 150428764 c/g g S 870407 44297 5150429097 c/t a R 870406 44311 5 150429111 c/t g R 6873202 44588 5150429388 a/g a R 8177414 44775 5 150429575 c/t c Y 8177415 44921 5150429721 c/t c Y 3805435 45006 5 150429806 g/a t Y 8177416 45098 5150429898 t/c c Y 3792799 45185 5 150429985 c/g c S 3792798 45475 5150430275 t/c g R 3828599 45506 5 150430306 t/c a R 8177417 45543 5150430343 g/c g S 3792797 45601 5 150430401 t/g a M 8177418 45652 5150430452 t/c c Y 8177419 45756 5 150430556 ag/g g N 8177420 45826 5150430626 t/c t Y 8177421 45974 5 150430774 c/g g S 4958872 46044 5150430844 t/c c Y 3792796 46200 5 150431000 c/g g S 8177422 46218 5150431018 a/g a R 8177423 46221 5 150431021 c/t c Y 4958434 46280 5150431080 c/t a R 8177424 46330 5 150431138 —/gagtcctgg gagtcctgg N8177425 46583 5 150431383 t/c c Y 8177426 46650 5 150431450 a/g a R8177427 46721 5 150431521 a/g a R 8177429 46808 5 150431608 g/c g S6889737 47242 5 150432042 c/a c M 3792795 47512 5 150432312 g/a c Y8177430 47600 5 150432400 t/c c Y 8177431 47706 5 150432506 a/g g R4958873 47806 5 150432606 a/g a R 8177432 47978 5 150432778 t/g t K8177433 48021 5 150432821 t/c c Y 8177434 48025 5 150432825 g/a a R8177435 48093 5 150432893 t/g g K 3763011 48413 5 150433213 t/c g R8177436 48933 5 150433733 c/t t Y 8177437 49097 5 150433897 c/g g S4958874 49105 5 150433905 t/c t Y 8177439 49570 5 150434370 g/a g R8177440 49591 5 150434391 c/t c Y 8177441 49704 5 150434504 g/c g S8177442 49705 5 150434505 a/t a W 8177443 49798 5 150434598 c/t c Y869975 50082 5 150434882 a/g g R 869976 50147 5 150434947 g/a a R8177444 50356 5 150435156 a/t a W 8177445 50725 5 150435525 t/c t Y7721469 50968 5 150435768 c/a a M 8177446 51029 5 150435829 a/c a M7704191 51086 5 150435886 t/c t Y 8177447 51166 5 150435966 t/c t Y11548 51493 5 150436293 c/t c Y 2230303 51539 5 150436339 g/t t K7722386 51562 5 150436362 g/a a R 8177448 51645 5 150436445 g/a g R8177449 51649 5 150436449 t/c c Y 2070593 51650 5 150436450 t/c g R8177450 51656 5 150436456 g/a a R 8177451 51657 5 150436457 t/c c Y8177452 52009 5 150436809 g/a a R 8177453 52143 5 150436943 a/c c M8177454 52349 5 150437149 g/c g S 3763010 52421 5 150437221 c/t c Y8177455 52532 5 150437332 a/g g R 8177456 52682 5 150437482 a/g g R736775 53058 5 150437858 t/c t Y 2277940 53187 5 150437987 t/c t Y8177458 53377 5 150438177 g/a g R 8177834 53699 5 150438499 g/a c Y 392453845 5 150438645 a/g g R 2233312 53920 5 150438720 a/g c Y 223331153929 5 150438729 t/g c M 2233310 55473 5 150440273 t/c g R 223330955690 5 150440490 a/g c Y 4958875 55850 5 150440650 a/g g R 223330856761 5 150441561 c/t g R 2233307 56840 5 150441640 c/g c S 223330657000 5 150441800 c/t g R 2233305 57116 5 150441916 t/g a M 223330458419 5 150443219 t/g c M 2233303 58420 5 150443220 c/t g R 223330258808 5 150443608 g/c c S 2287719 58906 5 150443706 a/g g R 228772059048 5 150443848 c/t c Y 7727034 59187 5 150443987 c/g c S 772725059361 5 150444161 c/t c Y 7709800 61218 5 150446018 g/a g R 384031261700 5 150446500 ag/a a N 2287721 62290 5 150447090 g/a g R 687529362596 5 150447396 t/c c Y 3805434 64049 5 150448849 g/c g S 208098266077 5 150450877 g/t t K 2080983 66079 5 150450879 g/t g K 228772266086 5 150450886 t/c c Y 2233301 66115 5 150450915 t/g c M 223330066150 5 150450950 c/g c S 4958876 66475 5 150451275 c/a a M 223329969177 5 150453977 a/g g R 2233298 69210 5 150454010 a/g c Y 228772369312 5 150454112 t/g t K 2161359 70244 5 150455044 a/g g R 773445670882 5 150455682 g/c g S 4292439 71905 5 150456705 t/c c Y 495887872294 5 150457094 a/t a W 6862024 72581 5 150457381 a/g g R 383481972786 5 150457589 —/ca ca N 2233297 72950 5 150457750 g/a t Y 223329673106 5 150457906 g/a c Y 2233295 73162 5 150457962 t/c g R 223329473273 5 150458073 g/t a M 7713028 74131 5 150458931 g/a g R 771322374406 5 150459206 t/c c Y 7713567 74665 5 150459465 c/t c Y 888989 747405 150459540 t/c c Y 2233293 75382 5 150460182 a/g c Y 3749657 75400 5150460200 g/t c M 2233292 75460 5 150460260 g/a c Y 2112635 75863 5150460663 c/t c Y 871269 76098 5 150460898 t/c c Y 3792794 78432 5150463232 a/g c Y 6579837 78604 5 150463404 g/t g K 3805433 79190 5150463990 c/g c S 5872186 79870 5 150464670 —/a a N 2233291 79928 5150464728 g/c c S 2233290 80213 5 150465013 g/c g S 2233289 80227 5150465027 c/t g R 4958435 81994 5 150466794 t/g g K 4958880 82187 5150466987 c/a c M 1422673 82698 5 150467498 t/c c Y 2042234 82841 5150467641 a/g a R 3805432 83214 5 150468014 a/g c Y 3805431 83249 5150468049 t/c g R 2233288 83485 5 150468285 t/c g R 2233287 83807 5150468607 c/t g R 3815720 83907 5 150468707 g/a g R 3792792 84216 5150469016 a/g t Y 3792791 84656 5 150469456 a/g c Y 2303018 85448 5150470248 g/a g R 3792790 85881 5 150470681 g/t a M 4958436 86539 5150471339 t/c t Y 2233286 86796 5 150471596 t/c g R 2233285 87057 5150471857 g/a c Y 7732451 87922 5 150472722 t/c a R 2233284 88098 5150472898 t/c g R 1422674 89319 5 150474119 g/t t K 3792789 89678 5150474478 c/t g R 4562032 90026 5 150474826 c/a c M 6865077 90033 5150474833 g/a g R 1559126 90114 5 150474914 c/g c S 3792788 90326 5150475126 t/c g R 1559127 90463 5 150475263 t/c t Y 3792786 90548 5150475348 a/g t Y 6880110 90800 5 150475600 a/g a R 6861227 90838 5150475638 g/t t K 3805430 91400 5 150476200 c/g c S 1862364 92086 5150476886 a/g a R 4958881 93946 5 150478746 t/c t Y 3792785 95360 5150480160 a/g t Y 6869605 96576 5 150481376 a/c a M 6870205 96721 5150481521 a/g g R 4246047 98316 5 150483116 t/a t W 4958882 98497 5150483297 c/g c S 3792784 99382 5 150484182 c/t a R 3792783 99442 5150484242 t/c a R 5872188 99764 5 150484565 —/ag ag N

Assay for Verifying and Allelotyping SNPs

The methods used to verify and allelotype proximal SNPs of Table 24 arethe same methods described in Examples 1 and 2 herein. The primers andprobes used in these assays are provided in Table 25 and Table 26,respectively. TABLE 25 DbSNP rs# Forward PCR primer Reverse PCR primer1478398 TTCCCTCCCTTCCTTTCTTC AAGATTACCCCACTGCACTC 1478397CACATACTTGTCTGAGGATC AGCACTCAGAATACTACCTG 1160114 AGCTGGATTCAGAGCCTTTGTCTGCTATCACCTTGTTCCC 1160113 TCTGCTATCACCTTGTTCCC AGCTGGATTCAGAGCCTTTG1382323 AGAAGGACAGTTGCAACAGG GTTCCTCATCCAGTCCTTAC 1160112GTTCCTCATCCAGTCCTTAC AGAAGGACAGTTGCAACAGG 7709870 TTATGACTCATAACTTTTTGCTTTCTTGAAATGCTCAGGG 7710643 ATAAAGTCTGGGCTCAGATG ATAGACCAGGGAACTGAGAC7730467 TGTGGCATATCTTCTATTGC AATGTGGCTGTTATGGTAGG 6579829GGAGGCTTTTTCACATTCAC AATGTGGCTGTTATGGTAGG 6579830 TGAGTATGGATTCTCAGGTGACCATAAGAGAGAAAAGCCC 6579831 GTGTGTGATGAATATGGGAG TTTCTCTCCTGTGTGTATTC6896232 GTCACACCTCTTTGTACATC CACTGAAAGCTCTCTCACAC 1351131AAGTGTGAGAGAGCTTTCAG GTATAAGGTTTCTCTCTAGTG 1038074 TCATATCTTGTGGGTTGTCCTTCACTGTGGACCACAAAGG 1478396 CATGGCCTCTTACAAGACTC GGGTAATTGTACCCACCAAG6880512 GAGGTATTGGTGATTAGGGC TAAAAAGGAATTCTTGGTGG 4958858GCAAGTGAGATCTTGGTAGG CAGACTGGCAGGAGTACATT 4958431 CAGAGGTGTCACTTCAGGAACCATCTTGCTTCTTTCCCTG 4958432 TGCCTTAATTCATGCAACCC TTATATCCAGGGAAAGAAGC6898463 CAAATCTACTTTTGAGTACC GACTCATCAAATGGAGAATG 4958859GTGGTATAACTCCTCAGAAG ATGATGAAGTTGTCTTCAGG 4130064 AATTCCTCACATTAACCCAGCCACAGCAAAGACAAAAGGG 4130065 CCACAGCAAAGACAAAAGGG TCCTCACATTAACCCAGGAG4133119 AGTGAGGTATAATGCCAGCC AAAATCTCCCTGTGTCTGCC 4958860TATGCGGTGGAGAAGAAAGG TCAACCCGGACAACAAAACC 4958861 ACTGGGATTTTCTGGTGAAGATGGGTGAGTCTCCCTTTAC 4437356 AGGTTTCTGGGTTTTCTCTC ATGGTAGTGTGTTTGTTTGC4958868 TTGTTGGCCTTGTTCATGTC CCTGTAATCCCTGCACTTTG 1478400TGGACTCCTGTGGGATAATG CAAGACTTCCAAGTGCATCC 6889375 TACCTGTCCACAGAAATAGCCCTGAGACCCTATGACTTTA 1600159 GGTTCATGTGATTTGGAAGC TGACTCTCCTAGTTCTTCAG6875892 GGGACTTCTCAGCTTCCAAA AGGGTTCTCCAGAGAAACAG 4608909CTGATAGAGGTTTATCTCAG TCTAGCCAAACTCCTATGTG C 2345000 TAACAAGCCCCTAACTAGTCTCAGGTGAAACCAACTGAAG 4516840 GTGCAGTGGATCCTTTTTCC CTAGACTCTAGGTAGGACAC2054440 CCCTTGAATGAGATGGAGAC TGGGAAAGGAAGGGAATGTC 707141ACAGTGCTAAGCACTTTCCC ACAGTAGACACACAGGATAA 707142 AGCCTGGCTTCTTCGTCTACATTTCCACCCTGGGCACTTC 841236 ATGTGTGCATGAGAGGAGAG CCAAAAAGGAGGAATGTGGG707143 CTTTTAAAGCCAGATGGACC CCACCCTGTTCTTACTGTAG 707144ACTGCACCAGACGCTTTCAC TTGGGCAAGGCGTGTTTCCT 6869405 TCTCTCATCATGGCTTGTGGCTGGCCAAGCGATAACACTA 707145 TAAAGTCTGGGGAGGTCAGG AGTGGCTGCTTTCGGAACAG707146 GCAACAAGAGCGAAACTCTG AAATGGGCAACATGGCAAAG 707148ACCTTTCATATACAGCTGGG CTGTGTTCCAGCATCATCTG 707150 ACTGATCTGGGCTAGGAATCAAGGACAGCCAATTAACACG 5872184 TAAAATGTTGTTTACCAGGG AAGGATGCCTATGTGACCAG3763015 GCCAAATATTGTTTTCCTTG ACATCGACCCTTTGTTGTAC 2042235TCCTTCTCTGATCTCAGTTG TTAGGAATGAAAAGCCACAG 3763013 AGAGACCTGAGATGCTACCCACAGGTGGACAACTGAGATC 2042236 AAAGGTGTTTCCAGCAGATA AAAATGTAGAGAGATGCCCG1946234 AACAAGTCATCTAGCCAGAG CTGGCTTAGAAGCAGAAAAT 1946235CCTGGCTTAGAAGCAGAAAA GGCACTGGAGATCACTAATC 1946236 GCACTGGAGATCACTAATCCAAACCACCTGGCTTAGAAG 8177402 TATCTGTGGCCAAACCACCT CACAGGGAGCAAATTTATT8177403 TTTCCTTGAGTCTTTGGGTC GCCTGTAGAAAAAGGTTAGC 8177404ATTAGGACTGTAGGGACAGA GCTAACCTTTTTCTACAGGC 8177405 ATTAGGACTGTAGGGACAGATTTTCTACAGGCATGTCAGC 8177406 ATGTCAGCTGTGATCCTTAT GAAAACCCCATTCTGGGTAG8177407 TGATGGAAGTCTCCATTCTG AATTTCCAGAGGACCATCGC 8177408GGGCTAATAGCTCCCTAGAA CGCCAGGTGTTTTTAAAACC 8177409 ATGCTTCCCAGAATGGAGACTCCATTACAGCCAGGGCAAG 6888961 TCCTCTTTTGGCTCCAAAGT TTCCATCAGTTCTAGGGAGC8177410 TCCTTTCGCACTTTGGAGCC ATAAGCAAATCTGGGCAGAG 8177411TGTGACCAATCCGCGGCCAA TGAAATCCCAGCCGCCTAGC 8177412 TGAAATCCCAGCCGCCTAGTTTCAAGCCCTCGGGTGTGAC 8177413 TGCAGGCGTCCTGCCTGCT TCACTCACCTTCGACTTCTC870407 GTGACATAGATGTAGCAAGG AGAATGACTAAGGGAGGAAG 870406CGGAGGTGACATAGATGTAG AGAATGACTAAGGGAGGAAG 6873202 TAGAAATGGCCACTTGGATTAGTGTTCTGCACTGTCACTC 8177414 GGGATGCTTGGAGAAGCTGA AATGAGGACAGTGGGTCCTG8177415 CCTCAGTCTATTTCCAGCAG ATAAGCATGCTGGGTCATCC 3805435GACAGTATGTACTGGGGTGG TTTGAGTCTATCCCTACACC 8177416 GAAGACCCAGAGGATTTTAGAGGGATAGACTCAAAGCGAG 3792799 GGACAGAATTTTGGAAACGG CTAAAATCCTCTGGGTCTTC3792798 ATTTTCCAGGTCAGGAATGG CCAAAACTGAAGGTTGGGAC 3828599AGTCAGTCCCAACCTTCAGT CAGGGCCCAATTGTATCTTC 8177417 AGTTATTCATGGAGTCCACGTTTTGGAAGGGTCAAAGAAG 3792797 GAGTCCATTTATCCTTCCCC ACGTGGACTCCATGAATAAC8177418 AAGTCCTGACTGGTTGTATG CTCAGAGAGAATAGGATGGG 8177419ATGCCCATCTCTGCTCCCAA TCCTAACCCAGAGCTAAGAC 8177420 ATTCCATTCTCCACATGCCCTAAGAGTTCAGATGTCTTAG 8177421 ATTGAAAGGGAGATGCTAGG TTGTAGAGACAAGGAGCGTG4958872 AGTCACCTAGAAACCAGCAG ACAAAAGGACTGCAGAAGCC 3792796CAATATGATGCTGTGAGAAG ATCTTACAATGGAGGAGCTG 8177422 ATCTTACAATGGAGGAGCTGTATGATGCTGTGAGAAGTGG 8177423 ATCTTACAATGGAGGAGCTG TATGATGCTGTGAGAAGTGG4958434 CTGCTGGTCTTGTGTCTTTC AGACCACTTCTCACAGCATC 8177424GGCTTGAGAGTTGGATGATG GGAGAGGAGAAAGACACAAG 8177425 AGAGCAAAACAGGCCTGGAGACCCATGGGCCACCTTCTGA 8177426 ATTTCGTTCCAAGAGACTCC ATATGTCCAGGGAGATAAGC8177427 TTATCTCCCTGGACATATGC GGCAAGGAGAACCCTATTTC 8177429CAGGTTGTCCTAATAATGGG ATGAAATAGGGTTCTCCTTG 6889737 CATGACAGAGGCAGCTCTTCTGAAGGGAGTGATATATGCT 3792795 GGCCACAAAGGACCTTAAAG TGACACAATTTGGCAGGTGG8177430 AGTAGGATGTGGCCTATGAG ATTGTGTCAATGATGCAATG 8177431CATGATCCTATCAAGCCATC GACCTGAGTTTCCTCATTTA 4958873 GCCAGTCCAAGACCACACAGTGGGAGACCTGGGCTCCAT 8177432 CCCGGAACATTAACACATGC TTACCAAATAGGGAAGCAGG8177433 GGACAGGAAAGATGAAGCTG ATGCAGGTACTGTTGCTTTT 8177434GGACAGGAAAGATGAAGCTG ATGCAGGTACTGTTGCTTTT 8177435 ATTGGGAGCCTCTGAATCACGGCCAGTGTGAAGGAAAAAC 3763011 GTGCTTTTTAAGCACTTAAT CTCCTAATTCCTTGTTTAAG8177436 CACTGTGTTCCGTGGTTTTG CCTAGAACATGTCCAAAGGC 8177437GAGATGGTAACTGAAGCTCC TGCCAAGAGAAGCACCAAAG 4958874 TGCCAAGAGAAGCACCAAAGGAGATGGTAACTGAAGCTCC 8177439 AGAGAAGCATCCCAGAAAAA TCCATGCTCTGCCTGGCAAT8177440 GCAATCACAGGCGAATTCCC GCATCCCAGAAAAAGCACTC 8177441TCCCCAGTTTCCTCAGTGTC AACAGAGCAGAGTGCGTTTG 8177442 TCCCCAGTTTCCTCAGTGTCAACAGAGCAGAGTGCGTTTG 8177443 TGCCAAAGATTATGGGAGGG GAGCAAGAACTATCCATCCC869975 CTTTCTCGGGCCTCAGTAGT CTGTCTCATCCACACCACTC 869976AGTGCATTCAGTTCTGGGCC AGTGGTGTGGATGAGACAGG 8177444 AGGAGAGTAGTTCTTGGTGGAGAAAGCTCCTCTCACATGG 8177445 TTCTCTCCATTGACATCCCC TCAACAGGTATGTCCGACCA7721469 ACTATTGTTCCAACTAGAGG ATGTTCACTCTGGGTCCTTC 8177446AGTTTGGGAGTTAGAAGACT CAGAAGGACCCAGAGTGAAC 7704191 TTCCTGGGGAATAGCCTGGCCGCTGGCAGTCTTCTAACTC C 8177447 TGGGAGGACAGGAGTTCTAG GCCTCAAGCAAGGTTGACAC11548 TGTTCAGGAAGAAATCCGTG GGGGCCTTGAGTGATAGGA T 2230303TGCCTGGCAGTACACAGAAC ACTATCTACCCATCACAGAC 7722386 CCTGTAGGCATGTGTGTAAAGCACAAATGGATGCATACAG 8177448 CAGATGGTACACATTCCCAG GCCTACAGGTATGCGTGATT8177449 CAGATGGTACACATTCCCAG GCCTACAGGTATGCGTGATT 2070593ACACATGCCTACAGGTATGC CAGATGGTACACATTCCCAG 8177450 CAGGCACACAGATGGTACACGCCTACAGGTATGCGTGATT 8177451 CAGGCACACAGATGGTACAC GCCTACAGGTATGCGTGATT8177452 TGGCCCTTGCTGTCACATCT TACATCCCCACCCCACAGTT 8177453AGGGGTGGCATCCCTGCCCA TGAGGGGCCAGCCCTTAGTG 8177454 TGCACCCACTGTAAAAAGTAAGTGAGGAGGAGAAGGTGG 3763010 AGTGGGTGCAAAGGTAATTG TGATCTCAGGGAGAATTGTG8177455 ATGTAGCCCTATTAGGGGTG AGCTAACTATCAGGTTGTTG 8177456CCCGGGAGTTTGAGGCTATA AGTGCAGTGGCTCTTCACAG 736775 TACTCTTGAGTAATGGTGACTGGCCTAGGCTCCTTCCACT 2277940 CGAATCATGGACATAAATCC TCAGGGAAAGGAAGAAAAGG8177458 ACAGTAGCCTTGCTGAAGCC CAGCTTCAGGCTGGCGCCC 8177834TCTTAGGATTGCTGCTCCTG GAAAAACTCCTTCCCTGCTG 3924 TCCCCAGTCCTGTAAACAGCGAGCCTCAGCTGGATGAGAG 2233312 ATTGTGTCATTTGGCTCCAC CACACCGTGCAAGTGGCTTC2233311 TTGTGTCATTTGGCTCCACC CGTGCAAGTGGCTTCTAGTT 2233310CACTGTTTTGGAAAGCTGGG TTATCTGCCAGCATTGCCTG 2233309 ATTTAGTATTGTCCTGGCACTATTCCGGCTGCGACAAAAC 4958875 GGGATAACTTGCTGAAGGTC GCTTAAGAGATTGGCTCTGG2233308 AGACACCTTCCAGAGAGGA AGAGCCAGGCCCTGGACAC 2233307TCTTCCATCTGGTGAGTCTG TCCTCTCTGGAAGGTGTCTG 2233306 CCACCCAGAACATCTCTGCATGGTGTGGCACCATGGCTG 2233305 CTAGCCTTCAGAGAGCTAAC TCTGGCTTTGGCCTGCAACA2233304 TGGCCCAGAGGGAAGCATCA CCTGGATCCAGGGCCTAAC 2233303TGGCCCAGAGGGAAGCATCA CCTGGATCCAGGGCCTAAC 2233302 ACCAGGGCCCTCCAATCCATAAATGCCCAGGTAAGAGTGA 2287719 CTGCAGCTTCTCCACTTGCT TCGTGAGCGCATGAATGAGG2287720 AGGGAGGGTGAAGAGAGGGA ATCTGTGACCCCAGCAGGAG 7727034AGAGTAGTTCTGTGTCCCTC TGGTTGATGGCTTTCAACGT 7727250 TTGGATCTGCTGGTTCTGAGATGTGAAACTACAGGCTAAG 7709800 TTGCTCATGACCTAGGCATC CTTTATCTTCCTACTCCACC3840312 GAAATGTGCTGGAAAGCAGG CCTCTCCAGTTCAGTTTTGC 2287721CCTTGAGTCCAGCTGCAATG GGGTTACCCTTCCTAAGCTG 6875293 ATGGACATTACTGTACCGACTGGCCCAGCCCTTACGTTCT 3805434 AAATAAGGCCCCTGGTGTCC GGGCATGGTCTTTTGCCTAG2080982 CTGCCTCTTTAGCAGCTTTG AACTTCTCCTCTGGGCCATA 2080983CTGCCTCTTTAGCAGCTTTG AACTTCTCCTCTGGGCCATA 2287722 CTGCCTCTTTAGCAGCTTTGAACTTCTCCTCTGGGCCATA 2233301 CACAGGGCATGCCTGGTCTT CCACTCCCCAAGGTTCAAAG2233300 TGAACAAGCAGTGGGACCAG AAGACCAGGCATGCCCTGTG 4958876TTTTACCTTGCCAGGAAAGC CAAAGAGGTGTGTGTGTTCC 2233299 CCAGCATCTTCACCTTCTTCTTTTGGTGAAGGCTCCTCTG 2233298 TTTTGGTGAAGGCTCCTCTG AGCATCTTCACCTTCTTCTC2287723 AATTATCTCACCCAGTCTCG TGGGAGGAATAGGGAGACAG 2161359ACAGCTCTTCCTTACAAAAG TTAGTGACCATTGGTGGATC 7734456 AATAGTCTAGAGAGTTCCCCCCTGGGTTCTATAAGATGTTG 4292439 CCCTGGACAGGTCTCTTTAC AGGAAGAGCATCTGGCTCGG4958878 AGAGTTTACCCAGGACATGC TAGAATGGAATACTAGTCCC 6862024GTGCTGGGACTAGAGGATAG TGTTGGCTAGTGTCTGTGTG 3834819 TGGGAACCACTCTCTAAGACGTGTTGAGTCGTTTGCTCAG 2233297 ATGAAGCCACAGTGACTACC GTGGTAGAAGGTTCTAGCTG2233296 AACCAAGGATGCTACAGAAG ATGGAAGGGACAGGCAAGAA 2233295TTGCCTGTCCCTTCCATCTT ATCCCAGGGAGGAAAATTTG 2233294 TGGGATGGAGGTAAACAGAGCAACTCATGCGATAGTGAGC 7713028 CTGGTGAAATACATCACCAC TTGAGTAGCTGGGACTACAG7713223 CTTTTGGATGGCCAAGGATG CCAGCTTCAGTTTCTGAATC 7713567AGCTGGTACCCACCAGATGA ATCCTGTGAGTGGCCACAATT 888989 CAGGGACTTGATTGGAGTCTACTCACAGGATGGAGAGCAA 2233293 TCAGAGAAGGGGTGAGGAGT AACCCAGAAATCAGTGCTGC3749657 AGAAATCAGTGCTGCACACC TAAGTTCAGGCCCCTCAGAGA 2233292TGAACTTACCGCAGCCTCTC CCAAGTTGGATAAGGGCCTG 2112635 TCCATCCCTTCTCCTTCATGGACTTCTCTGAGGAGATGG 871269 TTCCACAAACTTTGAGCTGG TAATTAATTCCTGAGCTCCC3792794 TCTGCTAGAAGGTAAGCTTG AGTGTGTATGAAGTGCCACG 6579837ATAGATCCTAGGCCCCTTAA AGGAAATAAACAAGGAGGGG A 3805433 CTTAGCCTTGTGGGCTTTAGAAGCCTCCGTTTTGCCATCT 5872186 TCCCGTATTTCCCCATTTCC GCTTTTTGTTCAGGGTGATG2233291 CCTTATCCTGTTCATCACCC AATCCACTGGTACCATGGGG 2233290CTGCAGGTGCAGCATCAGG TACTCAGGCCTTGGTGAAGA 2233289 TCAGGCCTTGGTGAAGAGTGATCAGGTTGCCGTCCTCAC 4958435 AAGTTCTGCTCATCCTTCCC GATCTCTGAGGCTCCCTGTA4958880 TCTGGAGGAATGGCAATGAC ATTTCCTCTTGTTAGAATGC 1422673ATGCTTGTGTTCACTGGTTG CATGCTAGTTAGCCAGACAG 2042234 GCACCTATTGCTCGCATAACTCTACCTTCTCCATTCTCCC 3805432 AAGGCCCAGGGCCCTGTAAG AACCTCCTTCCTGTGTGCTCCA 3805431 AAATCACCTGTCTTACAGGG TTTGGGTCTGTGGGCTTCCA 2233288ATATGTGAGCGAGAAGCACC ACAGAACCTGTTCAGATCCC 2233287 ATGACAGGCTTGTCGAGAATTTTTGAGTACACAGGGACCG 3815720 TGCTGGTTCAAGGTCAGCTA TAATGTGAGGTCATATCCCC3792792 TTCTCAGATCAGTTCACTCC AACACAGATGGCAGCTGTTAC 3792791GATTGCATAGTTCAGCATCC CATTACCCGTGTAACCTCAG 2303018 CAAATGTCACAGCATCTCCCTAAAACTTACACTGGATGGA 3792790 CATAAAAGCCCCATATCCCC AGGGAGGGCCAAGTCTCAGT4958436 GCCTTGGGGTCTTTATCAGC ATATGCCAGCTCCAAGTGAC 2233286ATGAATGGAAGGGTAGCCTG ATCCTTCACAGTGAACTCCC 2233285 TTCCAAAAGCTCCCCTAGAGACGGGGAGAAGCAGCACAC 7732451 CATCTCTCGTGCCCCAGAAA GCTGGAAGGCCCAGTTAATG2233284 TACATGGTGCATAGAGACAG ATATGCCTCTGCCCAAGTCA 1422674TGCTGATAATTCCTCAGCAC GTCCATTCACTCATCCATTC 3792789 GAGGATTCCTGATGAAACAGTTCACTGGGGACCTCAGAAG 4562032 ATTTTACAAGGGAGAAACTG CTGACTTTGCATCTTGGCTC6865077 CTGACTTTGCATCTTGGCTC ATTTTACAAGGGAGAAACTG 1559126CACCATACTCACACAGCCAG ATGGTGCTGCCCTACAAAGA 3792788 CCCTTCTCCCAAAGACTGACAGTGTGGGTAGCTGTCCTTT 1559127 ACTGTTTCTTAGTTCGGTGG GGTGACTTGTTTTTAACCTG3792786 TGAGGACAGAGGCAAGCAGA AACAGTGTGGGGGCTGCACTT 6880110TCAAGAACAGTGAAACAGAG TGGTGGACAAGAACCAGGAC 6861227 CATGCCTTCATGTGGTGTTGAGCAGTGTCCTGGTTCTTGT 3805430 TCCAGAGAGGGTTTTCCCAC GGGAGAAAGAAAGGCAGTGG1862364 AATCCCATGGCACACCACAG TGTAGTTTGTGGAACCAGGC 4958881GGACTGGAGGTCATCATAAC TTCCACCCAAGGATGAAAGG 3792785 TTTGTTCTACCACTAACCAGCTACATGGATGAGGAAGCAG 6869605 TTTGTCCAGCTTCTGTGTAC AGGTGTGAGTTACCTAAGGG6870205 GAATCATCAAGAAGGGACTG ACCCCATTCTTTTTGATGGC 4246047AAGCTGGTCTCAAACTCCTG TGTTATTTAGGCTGAGCGTG 4958882 TTGTTGGTGGCTGATGAAGGGCTCACTCATCTGGTTTAAG 3792784 ATGATTTTCCCATGACTGGC AAACAGCCAGCTGCTGCTCA3792783 AATCTGAAGAGTGACTGTCC AAAGACCTTGGCGGCCACCAT 5872188CCTTTGCCGACTTTGGTTTC AGACTTGGAAGAGAATGCCC

TABLE 26 dbSNP Extend Term rs# Primer Mix 1478398 TGAGACAGGGCCTCACTCTACT 1478397 GTCTGAGGATCAGTAATAATAC ACT 1160114 CTTAATTGCAATGCCTCTT ACT1160113 CTTGTTCCCTACCTAGC ACT 1382323 CTGACCAGGGTAACAAC ACT 1160112TCTCCAATCACCGTTGT ACG 7709870 CCTACAAGAAATATCAAGGC ACG 7710643GAGCATTACAGTTGTCAC CGT 7730467 TTGCCACTAAATAAATCCAA ACT 6579829CATTCACTATGCTCTTTTTTT ACT 6579830 ATTCCCTGCTGAAGGTT ACG 6579831AATATGGGAGAGCCTTC CGT 6896232 AATACACACAGGAGAGAAA CGT 1351131GAGAGCTTTCAGTGAAGT ACT 1038074 GTGGGTTGTCCACATAT ACG 1478396CAAGACTCACTGCAATTTA ACT 6880512 GGGCTTCAACATATGAAT ACG 4958858GAAGATGAAGGCCTGAG ACT 4958431 CTTCAGGAAAATCCGGC ACT 4958432AAATACAGTCAGCCCCC ACT 6898463 CTTCATTTCAGGTATTCTACT ACT 4958859TCAGAAGAGGAGAGCAC ACT 4130064 CCTTCCAAAATTTCCCT ACG 4130065GGGAAATTTTGGAAGGT ACT 4133119 GCCAAGTATCCTGTATCAA ACT 4958860AGGGCAACAATAAGGGA ACT 4958861 AAGGAGCAGAAGCATGA ACT 4437356CTGAGCAGCCCTAGTGA CGT 4958868 CTCCTGACCTCGTGATC ACG 1478400TGGGTGAAAAAATTGGTA ACT 6889375 CTGCTGATGATGTAGGAAT ACT 1600159GAAGCTGAGAAGTCCCG ACT 6875892 CCAATTCATTATAGGAAATTTTTT CGT 4608909GGTTTATCTCAGCATTGTTTAT ACT 2345000 CTAGTCTCCTTGTTCTCTTA ACT 4516840TCCTTTTTCCACCTTGC CGT 2054440 GTAAACATCCAAAAGCATTTC ACT 707141ACATCCTGAGATGAAGG ACG 707142 CAGGCAGCAGCAGCACA ACT 841236ACCTTCTCTGAGGGTATTCTA ACT 707143 CCCTTAGAGGTCATCTGGTC ACG 707144CCATACAAAATCCCCTTG ACT 6869405 TGACTGGGACCCAGGCT ACT 707145GGCCCAGCAGCCAGAAG ACG 707146 GAAAAGAAAGGCTAAGTGA ACG 707148ACTGAGGCCCAGAGAGG CGT 707150 GTCTGCGCCACCTTCCC CGT 5872184GGAGACTCAATGCCAGG ACT 3763015 CTGTGCTGGTGCTGGTG ACG 2042235ATCTCAGTTGTCCACCT ACT 3763013 TACCCCTGGATTGCTAC ACT 2042236AGGCACCTAGGGTCACA ACG 1946234 CACTGGAGATCACTAATC CGT 1946235CCTCAGTTTCCTCAGCT ACT 1946236 ACACAGGGAGCAAATTT CGT 8177402CCAAACCACCTGGCTTA ACT 8177403 GTCTTTGGGTCTTCATTT ACG 8177404TCCTCCCCATCATAAGG ACT 8177405 TTCCTCCCCATCATAAG ACT 8177406CTCTGTCCCTACAGTCC ACG 8177407 GCATTTCTTATTCCTATCGAG ACT 8177408GCTCCCTAGAACTGATG ACT 8177409 GAGACTTCCATCAGTTCT CGT 6888961CCATTACAGCCAGGGCA CGT 8177410 CTTTGGAGCCAAAAGAG ACT 8177411GCGGCCAAGCCGAGACC ACT 8177412 GATTGGCTGCAAGGGTC ACG 8177413TCCTGCCTGCTTTCCCT ACT 870407 GACCCTCGAGGTGGCAG ACG 870406AGATGTAGCAAGGCGAC ACG 6873202 CAGAGATCTGTGCTAGAA ACT 8177414TGTTCTCTGAAATTTCCTC ACG 8177415 AGCAGGTGTGTGGCCCA ACG 3805435TACTGGGGTGGGCTCTG ACG 8177416 TGCATGTTGGGAAGTTG ACT 3792799CGGAATCCTAGACTCATAA ACT 3792798 ATAGACAGGCCAGCACC ACT 3828599CAACCTTCAGTTTTGGAA ACT 8177417 ACGTTCTCCCCACTAGC ACT 3792797ATCCTTCCCCAGAGTGC ACT 8177418 CTCACCCTAAAACTTTTCTA ACT 8177419CTGCTCCCAAGTTCTTA ACT 8177420 ACATGCCCCATAACCCT ACT 8177421TCCCCAGTCCAACTTCA ACT 4858872 GCAGTTACTAGGATCCC ACT 3792796TTGCTGTGTCTCTGATG ACT 8177422 CATCAGAGACACAGCAA ACT 8177423CAGAGACACAGCAAAGC ACG 4958434 TCTCCTCTCCCGTCTTAT ACG 8177424TGGATGATGGGACTCAG ACG 8177425 GAACGAAATCTCATGTCAA ACT 8177426AGCAGATTCCCACAACC ACT 8177427 GACTTGCTCAGGGCCAC ACT 8177429CCTAATAATGGGAACTTGTAAA ACT 6889737 GGCAGCTCTTCTGCACT CGT 3792795TTCAAGGTTTCTCCTTTC ACG 8177430 ATGAGCCTACTCTGCCC ACT 8177431CATGGTTGACCTGGTTG ACT 4958873 ACAGGGAGTTAATGGCA ACT 8177432TGTTGCTTTTCCCTACC ACT 8177433 GCTGTTGTTACCAAATAGG ACT 8177434GAAGCTGTTGTTACCAAA ACG 8177435 GAATCACCATGTCCATAC ACT 3763011AAGCACTTAATATTAAGTACCC ACT 8177436 TGTGAAGATGATTATATAAGCC ACG 8177437AAGCTCCATCTTGCTGA ACT 4958874 TGGGGCTGAGGGAAAAT ACT 8177439TTTCACTTCCGGGAATT ACG 8177440 TCCCGGAAGTGAAAGGA ACG 8177441TCAGTGTCCCCTGGTCT ACT 8177442 CTCAGTGTCCCCTGGTC CGT 8177443GAGGGTTGTAACCTCAC ACG 869975 GGGCCTCAGTAGTTCCAGC ACT 869976CCAAAGAGAAAGAGCAGA ACG 8177444 GATAAATGTCCACCATGA CGT 8177445CTGGAAATTAGGGACAA ACT 7721469 ACCCTGACTAGGGTCTC CGT 8177446GCTGTGGCTCTAGAAGA ACT 7704191 ATAGCCTGGCCCTGAGA ACT 8177447AGTTCTAGAGCAGGGAT ACT 11548 CACACTATCTACCCATCA ACG 2230303GTACACAGAACTGTATGC CGT 7722386 ACCCACACCCACATGCC ACG 8177448CATAGGTAGACACGTGG ACG 8177449 AAGACACATAGGTAGACAC ACT 2070593GCATGGGTGTACAGCCAC ACT 8177450 CCAGAAAGACACATAGG ACG 8177451CCCAGAAAGACACATAG ACT 8177452 TGTCACATCTGCCTTGG ACG 8177453CCTGCCCAGGGGCCTTA ACT 8177454 TCTTTTCTCATCCTCCC ACT 3763010AAAGGTAATTGCGGTTTT ACG 8177455 GGTGGCATCTTCATGAG ACT 8177456TGAGGCTATAGTGTGCC ACT 736775 TCCCTGAGGGTAGGGCA ACT 2277940CATGTTCATTTATGCGCT ACT 8177458 GCCATGCCAGCCACGTC ACG 8177834CCTGGAGGCTTCTGCAA ACG 3924 CTCAGTTCAGGGACTGGT ACT 2233312TGATCTCAGATTGCCAA ACT 2233311 CAGAGCCAGCTGATCTCA ACT 2233310CCAAGAGCAGAAACTAAC ACT 2233309 TGCTATTGTAATTTTTGGGT ACT 4958875CTGAAGGTCATCTAGCAA ACT 2233308 CTTCTGAACTAAACAGCAC ACG 2233307GCTCCAGGGCACACACA ACT 2233306 CTACCCCTACGCCTACC ACG 2233305GATGAGATGGGTGTCCT ACT 2233304 TCAGCCGGTCAGTCCTC ACT 2233303ATCAGCCGGTCAGTCCT ACG 2233302 TCCATGCCCCCTCTCCC ACT 2287719CTCCACTTGCTTCTTCA ACT 2287720 TTCTCCTCAGGCCCAGA ACG 7727034ACATTCTGGGCTTCAAG ACT 7727250 AGAGACCCTACAAACTC ACG 7709800CCTAGGCATCTCCCTGT ACG 3840312 CTAGAGCCGTTCCCACC CGT 2287721CCCATCACTGGCACCCC ACG 8875293 GAGCATTGTGAAGTGATG ACT 3805434TGCTGACGGGAGGAACT ACT 2080982 AACCTTGGGGAGTGGCC CGT 2080983TGAACCTTGGGGAGTGG CGT 2287722 GCAGCTTTGAACCTTGG ACT 2233301TGCCTGGTCTTCATCTC ACT 2233300 GGTAATGTGGGGTTCCT ACT 4958876CTAAGACATGGAAACTACAT CGT 2233299 TCACCTTCTTCTCGGCTGC ACT 2233298CCCCAGGCTAGTGTGAC ACT 2287723 AAGACCCCTCACCCAAG ACT 2181359CATTCCATTACTACAGTAATAACT ACT 7734456 CCCTTCCTTTACTTCCC ACT 4292439CCCTTTAATCTCCACTC ACT 4958878 CAGGACATGCAAATCTATT CGT 6862024CTAGAGGATAGGGGAGT ACT 3834819 CTTGACAAACTGGAATGT ACT 2233297ATGGAGGTCAGGCCAAG ACG 2233296 ACATACCTGCTGCTGTC ACG 2233295AGCCTGGCCGCCCAGAC ACT 2233294 CCCTGAGTTAAGAAACCT CGT 7713028TACATCACCACCCCTCC ACG 7713223 GGGGTTTTTATAGCCCTA ACT 7713567ACCAGATGACTCATCAC ACG 888989 GATTGGAGTCTTACAACA ACT 2233293GGGGCTGCAGGAGGAGG ACT 3749657 CCTCCTCCTGCAGCCCC CGT 2233292TCTCGGCAGCCTGATCC ACG 2112635 CCCCAGCTGACTTCTAC ACG 871269CTGGCAGTGAACAAGAG ACT 3792794 TGTTGTATTCACTGTTGC ACT 6579837AGGGGTCCTGCCAACCT CGT 3805433 TGGGCTTTAGCAGCGGG ACT 5872186CCTCATCCCTCCCCACA CGT 2233291 GTCTGCCCACAACACAG ACT 2233290GTTGCCGTCCTCACGGG ACT 2233289 CCCTTTCCCTCTCCAGG ACG 4958435ATGGGAATCACAGGACA ACT 4958880 GAATGGCAATGACTATAACC CGT 1422673TGTCAGTGCCCAGTGCA ACT 2042234 CTCGCATAACAACAATTAAAG ACT 3805432CCTGTAAGACAGGTGATTT ACT 3805431 GGGCCCTGGGCCTTCCT ACT 2233288CCCCAAGTGACTCCAGG ACT 2233287 AATCCTGCTTTGTGATCT ACG 3815720AAGGTCAGCTAGTCCCA ACG 3792792 GGGAAGGCCAGCACAGG ACT 3792791AGGTGCAGGGAAAGAAG ACT 2303018 CAGCATCTCCCACAGCC ACG 3792790GCTTGGGAAGGTCATCA CGT 4958436 TGTGTGCCAGGCATCGC ACT 2233286GATAAACGAGAGAATGTGG ACT 2233285 GGAAGCAAAAGCATTTACT ACG 7732451GTGCCCCAGAAAGAGGAG ACT 2233284 GTGAGGCAGGAGCCAGC ACT 1422674CACCCAGACTGGTGCCT CGT 3792789 CCTTGGGAGGAAGGTGC ACG 4562032TAACTCAGCCACGATGA CGT 6865077 GCATCTTGGCTCTTATTG ACG 1559126ACAGCCAGGACACAGAG ACT 3792788 CAGATACAAGATGAATACACC ACT 1559127TCGGTGGAAACATCTGC ACT 3792786 GACCCCCAATGTCTGCC ACT 6880110GCCACCTTGCATGACAA ACT 6861227 GAGTTCCATTTAGGGTG CGT 3805430CAATAGGATATTTCTCCTGC ACT 1862364 CCCCACCACATCTTCTC ACT 4958881GCAATGTGATATCATGGC ACT 3792785 TCTCAGGCAAATGACTT ACT 6869605CTCTAGATTCCTAGATAGGG ACT 6870205 ACAAACCACCATTCATTC ACT 4246047TGCTCTGCCTCCCAAAG CGT 4958882 TGATGAAGGAGAAATTTCAA ACT 3792784TCACTCTTCAGATTGGAA ACG 3792783 AAATCATCAAGAACTTCCC ACT 5872188GAATGAAAATGTTTCACTCT ACT

Genetic Analysis

Allelotyping results are shown for female cases and controls in Table27. The allele frequency for A2 allele is noted in the fifth and sixthcolumns for control pools and case pools, respectively, where “AF” isallele frequency. Some SNPs do not have an allele frequency disclosedbecause of failed assays. TABLE 27 Position in Low BMD dbSNP SEQ IDChromo-some A1/A2 Control AF Case AF Associated rs# NO: 5 PositionAllele (High BMD) (Low BMD) p-Value OR Allele 1478398 231 150385031 A/GA = 0.28 A = 0.34 0.0334 1.31 A G = 0.72 G = 0.66 1478397 330 150385130T/C T = 0.61 T = 0.67 0.0621 1.29 T C = 0.39 C = 0.33 1160114 582150385382 G/C G = 0.96 G = 0.92 0.0034 0.44 C C = 0.04 C = 0.08 1160113589 150385389 A/G A = 0.96 A = 0.93 0.0169 0.51 G G = 0.04 G = 0.071382323 1060 150385860 A/G A = 0.67 A = 0.63 0.1288 0.83 G G = 0.33 G =0.37 1160112 1066 150385866 G/A G = 0.91 G = 0.85 0.0025 0.57 A A = 0.09A = 0.15 7709870 1311 150386111 G/A G = 0.11 G = 0.12 0.5796 1.11 G A =0.89 A = 0.88 7710643 1556 150386356 G/T G = 0.87 G = 0.86 0.7100 0.93 TT = 0.13 T = 0.14 7730467 1655 150386455 T/C T = 1.00 T = 1.00 C = 0.00C = 0.00 6579829 1692 150386492 A/C A = 0.92 A = 0.89 0.1423 0.74 C C =0.08 C = 0.11 6579830 1802 150386602 G/A G = 0.91 G = 0.89 0.4377 0.86 AA = 0.09 A = 0.11 6579831 2061 150386861 T/A T = 0.31 T = 0.32 0.79481.04 T A = 0.69 A = 0.68 6896232 2112 150386912 T/A T = 0.90 T = 0.890.7880 0.94 A A = 0.10 A = 0.11 1351131 2153 150386953 T/C T = 0.51 T =0.49 0.4152 0.91 C C = 0.49 C = 0.51 1038074 2667 150387467 C/T C = 0.23C = 0.27 0.1062 1.25 C T = 0.77 T = 0.73 1478396 3115 150387915 T/C T =0.92 T = 0.90 0.1123 0.71 C C = 0.08 C = 0.10 6880512 3186 150387986 G/AG = 0.64 G = 0.60 0.2313 0.86 A A = 0.36 A = 0.40 4958858 5621 150390421T/C T = 0.06 T = 0.13 ˜0.00001 2.54 T C = 0.94 A = 0.87 4958431 5735150390535 T/G T = 0.98 T = 0.95 0.0132 0.44 G G = 0.02 G = 0.05 49584325829 150390629 G/C G = 0.86 G = 0.85 0.4357 0.87 C C = 0.14 C = 0.156898463 6658 150391458 A/C A = 0.86 A = 0.82 0.0341 0.71 C C = 0.14 C =0.18 4958859 7901 150392701 G/C G = 0.95 G = 0.90 0.0030 0.48 C C = 0.05C = 0.10 4130064 11447 150396247 G/A G = 0.26 G = 0.30 0.1514 1.21 G A =0.74 A = 0.70 4130065 11466 150396266 A/G A = 0.16 A = G = 0.84 G =4133119 11984 150396784 T/C T = 0.95 T = 0.94 0.2216 0.72 C C = 0.05 C =0.06 4958860 15803 150400603 T/G T = 0.10 T = 0.17 0.0163 1.88 T G =0.90 G = 0.83 4958861 16257 150401057 T/G T = 0.13 T = 0.17 0.0581 1.38T G = 0.87 G = 0.83 4437356 17604 150402404 C/A C = 0.10 C = A = 0.90 A= 4958868 19762 150404562 C/T C = 0.55 C = 0.53 0.6623 0.94 T T = 0.45 T= 0.47 1478400 22367 150407167 A/G A = 0.96 A =0.94 0.0584 0.57 G G =0.04 G = 0.06 6889375 22709 150407509 A/G A = 0.59 A = 0.61 0.5378 1.09A G = 0.41 G = 0.39 1600159 23631 150408431 G/C G = 0.62 G = 0.61 0.73400.96 C C = 0.38 C = 0.39 6875892 23686 150408486 T/A T = 1.00 T = 1.00 A= 0.00 A = 0.00 4608909 25599 150410399 T/C T = 0.98 T = 0.92 0.00080.27 C C = 0.02 C = 0.08 2345000 26973 150411773 A/C A = 0.57 A = 0.550.5708 0.93 C C = 0.43 C = 0.45 4516840 28457 150413257 G/T G = 0.00 G =0.00 T = 1.00 T = 1.00 2054440 28669 150413469 A/G A = 0.62 A = 0.660.1835 1.18 A G = 0.38 G = 0.34 707141 29908 150414708 C/T C = 0.45 C =0.46 0.6490 1.06 C T = 0.55 T = 0.54 707142 30105 150414905 A/G A = 0.54A = 0.53 0.7349 0.96 G G = 0.46 G = 0.47 841236 30711 150415511 A/G A =0.55 A = 0.54 0.7731 0.97 G G = 0.45 G = 0.46 707143 30851 150415651 G/AG = 0.64 G = 0.66 0.6049 1.07 G A = 0.36 A = 0.34 707144 31203 150416003T/C T = 0.69 T = 0.75 0.0163 1.38 T C = 0.31 C = 0.25 6869405 31446150416246 A/C A = 0.08 A = 0.09 0.5734 1.13 A C = 0.92 C = 0.91 70714531638 150416438 C/T C = 0.62 C = 0.61 0.8223 0.97 T T = 0.38 T = 0.39707146 33064 150417864 C/T C = 0.53 C = 0.48 0.1214 0.83 T T = 0.47 T =0.52 707148 33958 150418758 C/A C = 0.46 C = 0.48 0.6249 1.06 C A = 0.54A = 0.52 707150 35182 150419982 A/T A = 0.67 A = 0.67 0.9445 0.99 A T =0.33 T = 0.33 5872184 38332 150423132 —/C — = 0.00 — = 0.00 C = 1.00 C =1.00 3763015 40875 150425675 G/A G = 0.61 G = 0.60 0.7976 0.97 A A =0.39 A = 0.40 2042235 41624 150426424 T/C T = 0.13 T = 0.20 0.0014 1.70T C = 0.87 C = 0.80 3763013 41671 150426471 A/G A = 0.97 A = 0.82˜0.00001 0.14 G G = 0.03 G = 0.18 2042236 41825 150426625 G/A G = 0.90 G= 0.86 0.0420 0.69 A A = 0.10 A = 0.14 1946234 42920 150427720 C/A C =0.00 C = 0.00 A = 1.00 A = 1.00 1946235 42935 150427735 T/C T = T = 0.87C = C = 0.13 1946236 43001 150427801 T/A T = 0.19 T = 0.20 0.5482 1.10 TA = 0.81 A = 0.80 8177402 43012 150427812 T/C T = 0.00 T = 0.00 C = 1.00C = 1.00 8177403 43203 150428003 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.008177404 43294 150428094 T/C T = 0.97 T = 0.94 0.0363 0.52 C C = 0.03 C =0.06 8177405 43295 150428095 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.008177406 43344 150428144 C/T C = 0.09 C = 0.11 0.1222 1.36 C T = 0.91 T =0.89 8177407 43509 150428309 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.008177408 43549 150428349 G/C G = 0.00 G = 0.00 C = 1.00 C = 1.00 817740943560 150428360 T/A T = 0.23 T = 0.28 0.0779 1.29 T A = 0.77 A = 0.726888961 43578 150428378 A/T A = 0.08 A = 0.07 0.3176 0.79 T T = 0.92 T =0.93 8177410 43640 150428440 A/G A = A = 0.00 G = G = 1.00 8177411 43792150428592 G/C G = 1.00 G = 1.00 C = 0.00 C = 0.00 8177412 43797150428597 C/T C = 0.15 C = 0.18 0.0943 1.31 C T = 0.85 T = 0.82 817741343964 150428764 C/G C = C = 0.01 G = G = 0.99 870407 44297 150429097 C/TC = 0.07 C = 0.11 0.0641 1.49 C T = 0.93 T = 0.89 870406 44311 150429111C/T C = 0.89 C = 0.88 0.5675 0.87 T T = 0.11 T = 0.12 6873202 44588150429388 A/G A = 1.00 A = 1.00 G = 0.00 G = 0.00 8177414 44775150429575 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 8177415 44921150429721 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 3805435 45006150429806 G/A G = 0.10 G = 0.07 0.1318 0.71 A A = 0.90 A = 0.93 817741645098 150429898 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 3792799 45185150429985 C/G C = 0.00 C = 0.00 G = 1.00 G = 1.00 3792798 45475150430275 T/C T = 0.05 T = 0.10 0.0906 2.06 T C = 0.95 C = 0.90 382859945506 150430306 T/C T = 0.55 T = 0.55 0.9993 1.00 T C = 0.45 C = 0.458177417 45543 150430343 G/C G = 1.00 G = 1.00 C = 0.00 C = 0.00 379279745601 150430401 T/G T = 0.46 T = 0.52 0.0520 1.27 T G = 0.54 G = 0.488177418 45652 150430452 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 817741945756 150430556 A/— A = 0.00 A = 0.00 — = 1.00 — = 1.00 8177420 45826150430626 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 8177421 45974150430774 C/G C = 0.07 C = 0.02 G = 0.93 G = 0.98 4958872 46044150430844 T/C T = 0.81 T = 0.81 0.9167 0.98 T C = 0.19 C = 0.19 379279646200 150431000 C/G C = 0.40 C = 0.41 0.5382 1.08 C G = 0.60 G = 0.598177422 46218 150431018 A/G A = 1.00 A = 1.00 G = 0.00 G = 0.00 817742346221 150431021 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 4958434 46280150431080 C/T C = 0.86 C = 0.83 0.1864 0.79 T T = 0.14 T = 0.17 817742446330 150431138 —/ — = 1.00 — = 0.98 GAGTCCTGG GAGTCCTGG = 0.00GAGTCCTGG = 0.02 8177425 46583 150431383 T/C T = 0.02 T = 0.03 0.50801.30 T C = 0.98 C = 0.97 8177426 46650 150431450 A/G A = 0.31 A = 0.360.0447 1.29 A G = 0.69 G = 0.64 8177427 46721 150431521 A/G A = 0.26 A =0.32 0.0296 1.33 A G = 0.74 G = 0.68 8177429 46808 150431608 G/C G =0.91 G = 0.95 0.0132 1.89 G C = 0.09 C = 0.05 6889737 47242 150432042C/A C = 1.00 C = 1.00 A = 0.00 A = 0.00 3792795 47512 150432312 G/A G =0.89 G = 0.97 0.0000 3.53 G A = 0.11 A = 0.03 8177430 47600 150432400T/C T = 0.01 T = 0.01 C = 0.99 C = 0.99 8177431 47706 150432506 A/G A =0.71 A = 0.70 0.6316 0.94 G G = 0.29 G = 0.30 4958873 47806 150432606A/G A = 0.31 A = 0.38 0.0279 1.32 A G = 0.69 G = 0.62 8177432 47978150432778 T/G T = 1.00 T = 1.00 G = 0.00 G = 0.00 8177433 48021150432821 T/C T = 0.26 T = 0.23 0.2375 0.85 C C = 0.74 C = 0.77 817743448025 150432825 G/A G = 0.00 G = 0.00 A = 1.00 A = 1.00 8177435 48093150432893 T/G T = 0.81 T = 0.79 0.4469 0.89 G G = 0.19 G = 0.21 376301148413 150433213 T/C T = 0.01 T = 0.02 C = 0.99 C = 0.98 8177436 48933150433733 C/T C = 0.00 C = 0.00 T = 1.00 T = 1.00 8177437 49097150433897 C/G C = 0.17 C = 0.07 ˜0.00001 0.38 G G = 0.83 G = 0.934958874 49105 150433905 T/C T = 0.44 T = 0.49 0.0880 1.24 T C = 0.56 C =0.51 8177439 49570 150434370 G/A G = G = 1.00 A = A = 0.00 8177440 49591150434391 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 8177441 49704150434504 G/C G = 1.00 G = 1.00 C = 0.00 C = 0.00 8177442 49705150434505 A/T A = 1.00 A = 1.00 T = 0.00 T = 0.00 8177443 49798150434598 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 869975 50082 150434882A/G A = 0.37 A = 0.25 ˜0.00001 0.57 G G = 0.63 G = 0.75 869976 50147150434947 G/A G = 0.00 G = 0.00 A = 1.00 A = 1.00 8177444 50356150435156 A/T A = 1.00 A = 1.00 T = 0.00 T = 0.00 8177445 50725150435525 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 7721469 50968150435768 C/A C = 0.00 C = 0.00 A = 1.00 A = 1.00 8177446 51029150435829 A/C A = 1.00 A = 1.00 C = 0.00 C = 0.00 7704191 51086150435886 T/C T = 1.00 T = 1.00 C = 0.00 C = 0.00 8177447 51166150435966 T/C T = 0.27 T = 0.35 0.0030 1.50 T C = 0.73 C = 0.65 1154851493 150436293 C/T C = 0.90 C = 0.95 0.0002 2.40 C T = 0.10 T = 0.052230303 51539 150436339 G/T G = 0.00 G = 0.01 T = 1.00 T = 0.99 772238651562 150436362 G/A G = 0.00 G = 0.00 A = 1.00 A = 1.00 8177448 51645150436445 G/A G = 0.99 G = 0.99 A = 0.01 A = 0.01 8177449 51649150436449 T/C T = 0.17 T = 0.14 0.2009 0.80 C C = 0.83 C = 0.86 207059351650 150436450 T/C T = 0.31 T = 0.28 0.2540 0.86 C C = 0.69 C = 0.728177450 51656 150436456 G/A G = 0.00 G = 0.00 A = 1.00 A = 1.00 817745151657 150436457 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 8177452 52009150436809 G/A G = 0.00 G = 0.00 A = 1.00 A = 1.00 8177453 52143150436943 A/C A = 0.00 A = 0.00 C = 1.00 C = 1.00 8177454 52349150437149 G/C G = 0.99 G = 0.98 C = 0.01 C = 0.02 3763010 52421150437221 C/T C = 0.76 C = 0.80 0.1337 1.30 C T = 0.24 T = 0.20 817745552532 150437332 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 8177456 52682150437482 A/G A = 0.45 A = 0.50 0.1098 1.22 A G = 0.55 G = 0.50 73677553058 150437858 T/C T = 0.56 T = 0.59 0.3945 1.12 T C = 0.44 C = 0.412277940 53187 150437987 T/C T = 0.92 T = 0.97 0.0004 2.75 T C = 0.08 C =0.03 8177458 53377 150438177 G/A G = 0.98 G = 0.98 0.9445 1.03 G A =0.02 A = 0.02 8177834 53699 150438499 G/A G = 0.93 G = 0.89 0.0113 0.58A A = 0.07 A = 0.11 3924 53845 150438645 A/G A = 0.75 A = 0.74 0.73500.95 G G = 0.25 G = 0.26 2233312 53920 150438720 A/G A = 0.00 A = 0.00 G= 1.00 G = 1.00 2233311 53929 150438729 T/G T = 0.15 T = 0.20 0.01061.49 T G = 0.85 G = 0.80 2233310 55473 150440273 T/C T = 0.00 T = 0.00 TC = 1.00 C = 1.00 2233309 55690 150440490 A/G A = 0.00 A = 0.00 G = 1.00G = 1.00 4958875 55850 150440650 A/G A = 0.56 A = 0.53 0.2131 0.86 G G =0.44 G = 0.47 2233308 56761 150441561 C/T C = 1.00 C = 1.00 T = 0.00 T =0.00 2233307 56840 150441640 C/G C = 0.00 C = 0.00 G = 1.00 G = 1.002233306 57000 150441800 C/T C = 1.00 C = 1.00 T = 0.00 T = 0.00 223330557116 150441916 T/G T = T = 0.27 G = G = 0.73 2233304 58419 150443219T/G T = 0.00 T = 0.01 G = 1.00 G = 0.99 2233303 58420 150443220 C/T C =1.00 C = 1.00 T = 0.00 T = 0.00 2233302 58808 150443608 G/C G = 0.98 G =0.87 ˜0.00001 0.14 C C = 0.02 C = 0.13 2287719 58906 150443706 A/G A =0.00 A = 0.00 G = 1.00 G = 1.00 2287720 59048 150443848 C/T C = 0.39 C =0.42 0.2852 1.14 C T = 0.61 T = 0.58 7727034 59187 150443987 C/G C =0.24 C = 0.30 0.0374 1.36 C G = 0.76 G = 0.70 7727250 59361 150444161C/T C = 0.33 C = 0.43 0.0003 1.57 C T = 0.67 T = 0.57 7709800 61218150446018 G/A G = 1.00 G = 1.00 A = 0.00 A = 0.00 3840312 61700150446500 G/— G = 0.54 G = 0.50 0.1346 0.84 — — = 0.46 — = 0.50 228772162290 150447090 G/A G = 1.00 G = 1.00 A = 0.00 A = 0.00 6875293 62596150447396 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 3805434 64049150448849 G/C G = 0.93 G = 0.87 0.0009 0.51 C C = 0.07 C = 0.13 208098266077 150450877 G/T G = 0.02 G = 0.01 T = 0.98 T = 0.99 2080983 66079150450879 G/T G = 1.00 G = 1.00 T = 0.00 T = 0.00 2287722 66086150450886 T/C T = 0.00 T = 0.00 C = 1.00 C = 1.00 2233301 66115150450915 T/G T = 0.00 T = 0.00 G = 1.00 G = 1.00 2233300 66150150450950 C/G C = 0.00 C = 0.00 G = 1.00 G = 1.00 4958876 66475150451275 C/A C = C = 0.95 A = A = 0.05 2233299 69177 150453977 A/G A =0.48 A = 0.49 0.7917 1.03 A G = 0.52 G = 0.51 2233298 69210 150454010A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 2287723 69312 150454112 T/G T =1.00 T = 1.00 G = 0.00 G = 0.00 2161359 70244 150455044 A/G A = 0.53 A =0.51 0.5813 0.94 G G = 0.47 G = 0.49 7734456 70882 150455682 G/C G =0.53 G = 0.60 0.0188 1.32 G C = 0.47 C = 0.40 4292439 71905 150456705T/C T = 0.87 T = 0.84 0.1343 0.77 C C = 0.13 C = 0.16 4958878 72294150457094 A/T A = A = 0.12 T = T = 0.88 6862024 72581 150457381 A/G A =0.80 A = 0.78 0.4512 0.89 G G = 0.20 G = 0.22 3834819 72786 150457589—/CA — = 0.00 — = 0.00 CA = 1.00 CA = 1.00 2233297 72950 150457750 G/A G= 0.04 G = 0.05 0.3215 1.32 G A = 0.96 A = 0.95 2233296 73106 150457906G/A G = 1.00 G = 1.00 A = 0.00 A = 0.00 2233295 73162 150457962 T/C T =0.00 T = 0.00 C = 1.00 C = 1.00 2233294 73273 150458073 G/T G = 0.24 G =0.23 0.7059 0.95 T T = 0.76 T = 0.77 7713028 74131 150458931 G/A G = 0.9G = 0.85 0.0238 0.66 A A = 0.1 A = 0.15 7713223 74406 150459206 T/C T =0.93 T = 0.88 0.0047 0.56 C C = 0.07 C = 0.12 7713567 74665 150459465C/T C = 0.61 C = 0.63 0.4135 1.10 C T = 0.39 T = 0.37 888989 74740150459540 T/C T = 0.90 T = 0.86 0.0352 0.67 C C = 0.10 C = 0.14 223329375382 150460182 A/G A = 0.00 A = 0.00 G = 1.00 G = 1.00 3749657 75400150460200 G/T G = 1.00 G = 1.00 T = 0.00 T = 0.00 2233292 75460150460260 G/A G = G = 1.00 A = A = 0.00 2112635 75863 150460663 C/T C =0.35 C = 0.36 0.7381 1.04 C T = 0.65 T = 0.64 871269 76098 150460898 T/CT = 0.47 T = 0.42 0.0796 0.81 C C = 0.53 C = 0.58 3792794 78432150463232 A/G A = 0.21 A = 0.10 ˜0.00001 0.41 G G = 0.79 G = 0.906579837 78604 150463404 G/T G = 0.89 G = 0.91 0.1630 1.33 G T = 0.11 T =0.09 3805433 79190 150463990 C/G C = 0.28 C = 0.28 0.9484 0.99 C G =0.72 G = 0.72 5872186 79870 150464670 —/A — = 0.33 — = 0.35 0.5526 1.08— A = 0.67 A = 0.65 2233291 79928 150464728 G/C G = 1.00 G = 1.00 C =0.00 C = 0.00 2233290 80213 150465013 G/C G = 0.14 G = 0.13 0.8704 0.97C C = 0.86 C = 0.87 2233289 80227 150465027 C/T C = 1.00 C = 0.99 T =0.00 T = 0.010 4958435 81994 150466794 T/G T = 0.68 T = 0.63 0.0549 0.79G G = 0.32 G = 0.37 4958880 82187 150466987 C/A C = 0.75 C = 0.84 0.00021.76 C A = 0.25 A = 0.16 1422673 82698 150467498 T/C T = 0.45 T = 0.32˜0.00001 0.59 C C = 0.55 C = 0.68 2042234 82841 150467641 A/G A = 0.91 A= 0.93 0.1485 1.37 A G = 0.09 G = 0.07 3805432 83214 150468014 A/G A =0.04 A = 0.07 0.0188 1.93 A G = 0.96 G = 0.93 3805431 83249 150468049T/C T = 0.06 T = 0.02 C = 0.94 C = 0.98 2233288 83485 150468285 T/C T =0.00 T = 0.00 C = 1.00 C = 1.00 2233287 83807 150468607 C/T C = 0.90 C =0.92 0.2555 1.32 C T = 0.10 T = 0.08 3815720 83907 150468707 G/A G =0.99 G = 0.97 A = 0.01 A = 0.03 3792792 84216 150469016 A/G A = 1.00 A =0.99 G = 0.00 G = 0.01 3792791 84656 150469456 A/G A = 0.00 A = 0.00 G =1.00 G = 1.00 2303018 85448 150470248 G/A G = 1.00 G = 1.00 A = 0.00 A =0.00 3792790 85881 150470681 G/T G = 0.49 G = 0.49 0.9686 1.00 G T =0.51 T = 0.51 4958436 86539 150471339 T/C T = 0.88 T = 0.92 0.0083 1.76T C = 0.12 C = 0.08 2233286 86796 150471596 T/C T = 0.00 T = 0.00 C =1.00 C = 1.00 2233285 87057 150471857 G/A G = 1.00 C = 1.00 A = 0.00 A =0.00 7732451 87922 150472722 T/C T = 0.90 T = 0.92 0.2349 1.29 T C =0.10 C = 0.08 2233284 88098 150472898 T/C T = 0.00 T = 0.00 C = 1.00 C =1.00 1422674 89319 150474119 G/T G = G = T = T = 3792789 89678 150474478C/T C = 0.42 C = 0.42 0.9873 1.00 C T = 0.58 T = 0.58 4562032 90026150474826 C/A C = C = 1.00 A = A = 0.00 6865077 90033 150474833 G/A G =1.00 G = 1.00 A = 0.00 A = 0.00 1559126 90114 150474914 C/G C = 1.00 C =1.00 G = 0.00 G = 0.00 3792788 90326 150475126 T/C T = 0.00 T = 0.00 C =1.00 C = 1.00 1559127 90463 150475263 T/C T = 0.94 T = 0.94 0.6080 1.14T C = 0.06 C = 0.06 3792786 90548 150475348 A/G A = 0.98 A = 0.99 G =0.02 G = 0.01 6880110 90800 150475600 A/G A = 0.91 A = 0.92 0.5433 1.14A G = 0.09 G = 0.08 6861227 90838 150475638 G/T G = 0.26 G = 0.23 0.18430.83 T T = 0.74 T = 0.77 3805430 91400 150476200 C/G C = 0.02 C = 0.05 G= 0.98 G = 0.95 1862364 92086 150476886 A/G A = 0.91 A = 0.92 0.55751.13 A G = 0.09 G = 0.08 4958881 93946 150478746 T/C T = 0.91 T = 0.910.9701 1.01 T C = 0.09 C = 0.09 3792785 95360 150480160 A/G A = 0.92 A =0.93 0.5448 1.16 A G = 0.08 G = 0.07 6869605 96576 150481376 A/C A =0.90 A = 0.89 0.7189 0.93 C C = 0.10 C = 0.11 6870205 96721 150481521A/G A = 0.00 A = 0.07 G = 1.00 G = 0.93 4246047 98316 150483116 T/A T =0.94 T = 0.96 0.2613 1.37 T A = 0.06 A = 0.04 4958882 98497 150483297C/G C = 0.88 C = 0.89 0.7723 1.06 C G = 0.12 G = 0.11 3792784 99382150484182 C/T C = 0.00 C = 0.04 T = 1.00 T = 0.96 3792783 99442150484242 T/C T = 0.85 T = 0.87 0.2391 1.22 T C = 0.15 C = 0.13 587218899764 150484565 —/AG — = 0.90 — = 0.91 0.5908 1.11 — AG = 0.10 AG = 0.09

Allelotyping results were considered particularly significant with acalculated p-value of less than or equal to 0.05 for allelotype results.These values are indicated in bold. The allelotyping p-values wereplotted in FIG. 4. The position of each SNP on the chromosome ispresented on the x-axis. The y-axis gives the negative logarithm (base10) of the p-value comparing the estimated allele in the case group tothat of the control group. The minor allele frequency of the controlgroup for each SNP designated by an X or other symbol on the graphs inFIG. 4 can be determined by consulting Table 27. For example, theleft-most X on the left graph is at position 150385031. By proceedingdown the Table from top to bottom and across the graphs from left toright the allele frequency associated with each symbol shown can bedetermined.

To aid the interpretation, multiple lines have been added to the graph.The broken horizontal lines are drawn at two common significance levels,0.05 and 0.01. The vertical broken lines are drawn every 20 kb to assistin the interpretation of distances between SNPs. Two other lines aredrawn to expose linear trends in the association of SNPs to the disease.The light gray line (or generally bottom-most curve) is a nonlinearsmoother through the data points on the graph using a local polynomialregression method (W. S. Cleveland, E. Grosse and W. M. Shyu (1992)Local regression models. Chapter 8 of Statistical Models in S eds J. M.Chambers and T. J. Hastie, Wadsworth & Brooks/Cole.). The black lineprovides a local test for excess statistical significance to identifyregions of association. This was created by use of a 10 kb slidingwindow with 1 kb step sizes. Within each window, a chi-square goodnessof fit test was applied to compare the proportion of SNPs that weresignificant at a test wise level of 0.01, to the proportion that wouldbe expected by chance alone (0.05 for the methods used here). Resultingp-values that were less than 104 were truncated at that value.

Finally, the exons and introns of the genes in the covered region areplotted below each graph at the appropriate chromosomal positions. Thegene boundary is indicated by the broken horizontal line. The exonpositions are shown as thick, unbroken bars. An arrow is place at the 3′end of each gene to show the direction of transcription.

Example 10 In Vitro Production of Target Polypeptides

cDNA is cloned into a pIVEX 2.3-MCS vector (Roche Biochem) using adirectional cloning method. A cDNA insert is prepared using PCR withforward and reverse primers having 5′ restriction site tags (in frame)and 5-6 additional nucleotides in addition to 3′ gene-specific portions,the latter of which is typically about twenty to about twenty-five basepairs in length. A Sal I restriction site is introduced by the forwardprimer and a Sma I restriction site is introduced by the reverse primer.The ends of PCR products are cut with the corresponding restrictionenzymes (i.e., Sal I and Sma I) and the products are gel-purified. ThepIVEX 2.3-MCS vector is linearized using the same restriction enzymes,and the fragment with the correct sized fragment is isolated bygel-purification. Purified PCR product is ligated into the linearizedpIVEX 2.3-MCS vector and E. coli cells transformed for plasmidamplification. The newly constructed expression vector is verified byrestriction mapping and used for protein production.

E. coli lysate is reconstituted with 0.25 ml of Reconstitution Buffer,the Reaction Mix is reconstituted with 0.8 ml of Reconstitution Buffer;the Feeding Mix is reconstituted with 10.5 ml of Reconstitution Buffer;and the Energy Mix is reconstituted with 0.6 ml of ReconstitutionBuffer. 0.5 ml of the Energy Mix was added to the Feeding Mix to obtainthe Feeding Solution. 0.75 ml of Reaction Mix, 50 μl of Energy Mix, and10 μg of the template DNA is added to the E. coli lysate.

Using the reaction device (Roche Biochem), 1 ml of the Reaction Solutionis loaded into the reaction compartment. The reaction device is turnedupside-down and 10 ml of the Feeding Solution is loaded into the feedingcompartment. All lids are closed and the reaction device is loaded intothe RTS500 instrument. The instrument is run at 30° C. for 24 hours witha stir bar speed of 150 rpm. The pIVEX 2.3 MCS vector includes anucleotide sequence that encodes six consecutive histidine amino acidson the C-terminal end of the target polypeptide for the purpose ofprotein purification. Target polypeptide is purified by contacting thecontents of reaction device with resin modified with Ni²⁺ ions. Targetpolypeptide is eluted from the resin with a solution containing freeNi²⁺ ions.

Example 11 Cellular Production of Target Polypeptides

Nucleic acids are cloned into DNA plasmids having phage recombinationcites and target polypeptides are expressed therefrom in a variety ofhost cells. Alpha phage genomic DNA contains short sequences known asattP sites, and E. coli genomic DNA contains unique, short sequencesknown as attB sites. These regions share homology, allowing forintegration of phage DNA into E. coli via directional, site-specificrecombination using the phage protein Int and the E. coli protein IHF.Integration produces two new aft sites, L and R, which flank theinserted prophage DNA. Phage excision from E. coli genomic DNA can alsobe accomplished using these two proteins with the addition of a secondphage protein, Xis. DNA vectors have been produced where theintegration/excision process is modified to allow for the directionalintegration or excision of a target DNA fragment into a backbone vectorin a rapid in vitro reaction (Gateway™ Technology (Invitrogen, Inc.)).

A first step is to transfer the nucleic acid insert into a shuttlevector that contains attL sites surrounding the negative selection gene,ccdB (e.g. pENTER vector, Invitrogen, Inc.). This transfer process isaccomplished by digesting the nucleic acid from a DNA vector used forsequencing, and to ligate it into the multicloning site of the shuttlevector, which will place it between the two attL sites while removingthe negative selection gene ccdB. A second method is to amplify thenucleic acid by the polymerase chain reaction (PCR) with primerscontaining attB sites. The amplified fragment then is integrated intothe shuttle vector using Int and IHF. A third method is to utilize atopoisomerase-mediated process, in which the nucleic acid is amplifiedvia PCR using gene-specific primers with the 5′ upstream primercontaining an additional CACC sequence (e.g., TOPO® expression kit(Invitrogen, Inc.)). In conjunction with Topoisomerase I, the PCRamplified fragment can be cloned into the shuttle vector via the attLsites in the correct orientation.

Once the nucleic acid is transferred into the shuttle vector, it can becloned into an expression vector having attR sites. Several vectorscontaining attR sites for expression of target polypeptide as a nativepolypeptide, N-fusion polypeptide, and C-fusion polypeptides arecommercially available (e.g., pDEST (Invitrogen, Inc.)), and any vectorcan be converted into an expression vector for receiving a nucleic acidfrom the shuttle vector by introducing an insert having an attR siteflanked by an antibiotic resistant gene for selection using the standardmethods described above. Transfer of the nucleic acid from the shuttlevector is accomplished by directional recombination using Int, IHF, andXis (LR clonase). Then the desired sequence can be transferred to anexpression vector by carrying out a one hour incubation at roomtemperature with Int, IHF, and Xis, a ten minute incubation at 37° C.with proteinase K, transforming bacteria and allowing expression for onehour, and then plating on selective media. Generally, 90% cloningefficiency is achieved by this method. Examples of expression vectorsare pDEST 14 bacterial expression vector with att7 promoter, pDEST 15bacterial expression vector with a T7 promoter and a N-terminal GST tag,pDEST 17 bacterial vector with a T7 promoter and a N-terminalpolyhistidine affinity tag, and pDEST 12.2 mammalian expression vectorwith a CMV promoter and neo resistance gene. These expression vectors orothers like them are transformed or transfected into cells forexpression of the target polypeptide or polypeptide variants. Theseexpression vectors are often transfected, for example, intomurine-transformed a adipocyte cell line 3T3-L1, (ATCC), human embryonickidney cell line 293, and rat cardiomyocyte cell line H9C2.

Representative Nucleotide and Amino Acid Sequences

Following is a genomic nucleotide sequence for a CTEP region. Thegenomic nucleotide sequence is set forth in SEQ ID NO: 1. The followingnucleotide representations are used throughout: “A” or “a” is adenosine,adenine, or adenylic acid; “C” or “c” is cytidine, cytosine, orcytidylic acid; “G” or “g” is guanosine, guanine, or guanylic acid; “T”or “t” is thymidine, thymine, or thymidylic acid; and “I” or “i” isinosine, hypoxanthine, or inosinic acid. Exons are indicated initalicized lower case type, introns are depicted in normal text lowercase type, and polymorphic sites are depicted in bold upper case type.SNPs are designated by the following convention: “R” represents A or G,“M” represents A or C; “W” represents A or T; “Y” represents C or T; “S”represents C or G; “K” represents G or T; “V” represents A, C or G; “H”represents A, C, or T; “D” represents A, G, or T; “B” represents C, G,or T; and “N” represents A, G, C, or T. >16: 56742951-56841200 1tgtctaaaaa ttattatggc tgaaacatac ttatcatttg ttttctgtag tagaaaatgg 61cataatatga gaaggcctac tttgtaatag ttgtttgctt tacttaatat ttttcaaatc 121agccatctgt gtgaagactc aatatcaagt taggccttta aaagggggaa aataattcaa 181aaccactggc agctgtggtt ttccRgcctt ttctattggt tgaactctgt aataaatgca 241ggaccctaca tatacaaaag gtactgtttt ccaaattcac gtatgtatta gcatttacct 301ttaaagttct catccgtcag aaaaacggtg aacacagcat tcagtatatt tattttggtt 361tagttacaca gtgtcaaaaa gttctgatgt atatggataa gtagatgcac tttttgctgt 421tttgtttgtt taaacagctt gctttaatta tctcaagcta cttggcagga acacctttat 481tccaggtttc gcataatcag ttattctggc aagagtagat taacacgtta gtgatctcca 541atagacagat tatatgttaa tattatttga ggccattatt tttaagtaca atttagacgg 601agtttcaaat agattttaaa aatgagattt gtataaaaga gtgtgcagtc cctatggtgt 661taaaatcact gatatggagg agaagagggg ttggaattgt gacactgtgg cagcagatga 721ccttggacaa gtcagttttt cagactttcc tcccttaaaa tagggatact aaatctcaca 781gggtggttgt gtataatgtc acccacacgc catctcctct ataactgcat ttgtatgcca 841ggaaattgtc tgtgaaggct tcagtttaga atagcttcat attattttgg ggcactgctg 901ggtgattttg gttaaggctg gccttgggag ttctctgagt tttctctgaa accaaactca 961atttggttgt aggttccctg tttgctgttt gttgctttta gtaataggag tcagtcagtt 1021tacctgggag ttaagtttgg actggagata atttgccatt cttttttttt tttttttttt 1081ttttttttga gatagggtct cactctgtcg cccaggctgg agtgcagtga catgatcttg 1141gctcactgca acctctgcct ccctggttca agcgattccc ctgcctcagc ctcctgagta 1201gtttgggtta caggcacaag ccaccacgcc cagctaattt ttgcattttt agtagagatg 1261gggtttcacc atgttggcca ggctggtctc gaacccctga cctcaggtga tctgcccgcc 1321ttggcctccc acagtgcttg gattacaggc atgagccacc atgcccagcc gataatttgc 1381cattctgtat ttatttgcgt gttagagacc agttaaagtt aagacacttt ctaaatagaa 1441gtcttgtata tggggctttt gagaaagtgt tttcccgtga tgctgggggg aaacacacat 1501attcaaatta cttgtataaa acagtttatt ctgtgtttag gaacttgtag gggtgaaatg 1561ctgacctgtg ttactctttc tttccatgat cagcRtccag aggaccagag gttaatttat 1621tctgggaagc tgttgttgga tcaccaatgt ctcagggact tgcttccaaa ggtacatcac 1681ttacacatta acttctgaat gtttttaagc actcaccagg ttaggttcag gtccttaagt 1741accttcgtat tacttttagc aggaaaaacg gcatgttttg catctggtgt gcaatgtgaa 1801gagtccttca aaaatgccag aaatcaacgc caaggtgtgt ctgcctcttc atgatggtaa 1861caatttgtat cattcagact ttcaggggag taataaaaga agttggataa aacgtttatt 1921gagagactgt atggtgttat taccaagtaa tattttgttt ctttcctagg aattagaaac 1981tgtattatca acacaaagtg atgagttaat agtagcagat tgttccgtta gtgctgtagt 2041agaaaggtgg agttacatat tgcactatct tatgggatat gatttgatct atggttactg 2101tgtatctaga ttactgaact aacactaatc gggtttttat tgaatttagc acatagcaag 2161tttcttcaaa ggagtcacta atttttataa agagtacaaa agtgaatata tttcctttgg 2221aaattttcat tgtgattagt tggataggaa aagatcagag tttttatcaa gtgatcttta 2281aagaattttt tttttttaaa aatggtctcg ctgtgtggcc caggcttttc tcaaactcct 2341gagggcaagc gatcctccca cctcagcctc ctgagtagct gggactacag gcatgtgcca 2401ctagacctgg ctctaaagac atatatgaca cacgaaacca tttatttttc atttcacaat 2461gtttattcac atatatggta ttagtattct aatgtagtga tgcactctaa atttgcatta 2521tatttcctag aacatctgaa cagagcatag gaaattccct attttgccat tatcagttct 2581aacaaaaaac ttaaaagcac tttatcattt catttccctg cactgtaatt tttttaaatg 2641atcaaaaacR gtatcatacc aaggcttact tatattggaa tactatttta gaaagttgtg 2701ggctgggttg tatttataaa tcttgttggt cagatgtctg caatgagtaa atttagcacc 2761attatcagga agctttctca ccaatgacaa cttcattgga agattttaat gaaagtgtag 2821catactctag ggaaaaaata tgaatatttt agcatctatg tattgaaaat tatgttgaat 2881aaatgtcaga ctatttttta cataacgttg cttctgttta attttgtcac gttcagaggt 2941ggggggtagg agatgtaagc ccttgacagc aaaataattc cttttgcttg atttcagaca 3001gttgcatcag ctcctttgtt ctgtgttcat gttacactta tttaggtggc tgaatccaca 3061gaggagcctg ctggttctaa tcggggacag tatcctgagg attcctcaag tgatggttta 3121aggcaaaggg aagttcttcg gaacctttct tcccctggat gggaaaacat ctcaaggtga 3181gtgttataat aaagatcttg gcttatgcaa catgaatgtt cctcgtttgc atcaatttaa 3241gaataaggta tgtttacacg tatataatca gaacttttaa acatacagaa ttttgcttta 3301taaatagctt cgctttaaag atctcttata tatttaactt ttcttaatac acagcctttt 3361agtacacaca aatttaaaaa gtaggtaatg catatattga aaaaaaaaag aaaatgtagg 3421cgttttatcc ttccattgtg ctgaccgctt ggttgccgtc atgataggaa attagtgctg 3481ctgcaggaga aaacagctgt cgtaagcatt gtgcagctgc tttgctgagt ggctttgtgc 3541tttattgtaa caatgggtga caacaaggga gagactgttt aagaagtgtc cttccaaaga 3601cttgggggta ctaggaaaat tggccaattt cttataacta ttaaagcttt tctttagagc 3661aaaagtcaga actaaatgct ctgttatttg gatttttata gctcttggaa tacattgttt 3721ttggggaaaa aftcattaac tgcaagttgc aattctgtaa ctctccaatt ctccgtcccc 3781ctttttctag tactttttat acacaatatt ttataaagcc aggtgtttta ggaatgagtt 3841tttcttcctt ttccccctta atggaacttt caaatataca caacagtggg gactggataa 3901ttaatcccca caccatcacc caggcctaat aaataacaat cagagtttta ccatagctca 3961tctattaccc tttccctttt ttttaaaaaa agagtattta aaagtaattc ccaggcatca 4021tgttatttca tccctatatt cttcagtata taactatgaa aaccttttag ttatcttgta 4081tatctagaat gccattatca gatctaacag aaatcttaaa agcactttat cattttattt 4141ccatgcactg taattttctt aaatgatcaa aaacagcatc ataccaaagc ttacttgtat 4201tggaatacta ttttagaaat actctgtgta tggaatttag ttaaaagatt gtcagcatat 4261aaattggata attattggat agaaaattat tggttagaaa aacttaaaac tacttccctt 4321attctgattc aactattctt aacttgagaa ttgaggctca tatttgactc tctgaatctc 4381accatattta aacttgattt cctttaacaa atatttattg aacagccagt acatacaact 4441ttgcttaagg atatgagttg acctgcagga atgaccacat aaccaatttc tgatctttgg 4501gcaattctat tatggtttca attttgtgat gtgctactga agaaattgac tgtgtaagaa 4561gcacaaagcc aaagatgact cgcatacacc tgccctgtga agttgagggc tgtgtggcct 4621gattaaagaa gcatgcattt ggccgggcgc ggtggctcac acctgtaatc ccagcacttt 4681gggaggccaa ggtgggcgga tcacgaggtc aggagattga gaccatcctg gctaacacgg 4741tgaaaccccg tctgtactaa aagtacaaaa aattagccag gtgtggtggc gtgtgcccgt 4801agtcccagct actggggagg ctgaggcagg agaatggtgt gaatccggga ggtggagctt 4861gcagtgagcc gagattgcgc cattgcactc caggctgggc aacagagcga gactccatct 4921caaaaaaaaa aaaaaagaag catgcatttg acatcagacc agctctgaaa tccagccctg 4981ctacttacta gctcagtgac cttgtgcaaa gcccctgtct tccctgagct gtacttgggt 5041tccccttgta aaatctcctt catgagattg ctgtggaccc agcagcctgt acaggacccc 5101cggtggtcag taagcatgca caggtgatac cacatgcaca cttcactata gattcagatg 5161gaggatggag gaagaagtgg atattgggag gatgggagag atttccttca ggaaagactg 5221ggagaaatgt ggcttttgag ctgctctcta aaagatgaac agtgggttga gacctgcagt 5281gagactcaac aagtcaaaac tgaggtggga tctacttaac ccaaaaaggt tgtttatata 5341tctgtgcact cagcaagcag gtggcaaggg ctgttgtttt cctgtgggat gggactgtgg 5401tagaagtgct gtgtcattta gtaccttctg tgcttgtggt acatgggtgc gtcatacagt 5461catttaccat gtaactgccg taaattccat tcttcYgtct cttcgatttg aattcttgat 5521tttaatttaa acttagtttt catttgttca tactcaaccc actcaaaatg agtgtttttt 5581caaatctatg tataggcctg aagctgccca gcaggcattc caaggcctgg gtcctggttt 5641ctccggttac acaccctatg ggtggcttca gctttcctgg ttccagcaga tatatgcacg 5701acagtactac atgcaatagt gagtccttcc cgccatgctg ggtgtggcca gggctcccgg 5761gaattgaagg gaattttatc cRtgttacct gtaaagttca gaatggaggg atgagtgtat 5821tttcatcagt ctgcagagcc ctgctctgtt tggtctgtgg gtagagtgta aatgacagct 5881gctacctgat ttgtgtattg acacaagRgt gcttttgtct gatacatagt ttagtgactt 5941tatttagaac atgttacttt cttttcaatt tgtgctatct tcaaagtttt gtgaggattt 6001taatttgcta ctgtgccata atcttacagt gggcttgatg tttaatttca ggtgaaattc 6061accttgtggc ttttcttaga aaaggcattt atagatatag ttagtaagga agttcttcac 6121taaattgaag aaaaatcaaa ggcatatcta cctacttgaa attcttaaca gtaaaagact 6181gctgtgtaaa taagccacag acttcacggt gctttggtct caacagtatc tgcctctgtc 6241gtttttattt tagtttagca gccactgctg catcaggggc ttttgttcca ccaccaagtg 6301cacaagagat acctgtggtc tctgcacctg ctccagcccc tattcacaac cagtttccag 6361ctgaaaacca gcctgccaat cagaatgctg ctcctcaagt ggttgttaat cctggagcca 6421atcaaaattt gcggatgaat gcacaaggtg gccctattgt ggaagaagat gatgaaataa 6481atcgagattg gttggattgg acctattcag cagctacatt ttctgttttt ctcagtatcc 6541tctacttcta ctcctccctg agcagattcc tcatggtcat gggggccacc gttgttatgt 6601acctgtaagc agatggtttc tctaatataa attacactac actgtgttca cactaagcag 6661attttgctct ttttgtttct tgtttttttt gagatggagt ctcgctctgt cattcaggct 6721ggagtacaat ggcacaatca ctgcaatctc cgcttcccag gttcaagcga ttctcctgcc 6781tcagcctcct gggtagttgg gattacaggc gcccaccacc atgcctggct aatttttata 6841tttctagtag agacggggtt tggtcatgtt ggccaggtgg tctcgaactc ctgacctcaa 6901gtgatctgcc caccttggcc tcccaaagtg ctggtattac aggcgtgagc caccactgcg 6961cctggccaga ttttgctctt ttttgagcag tctcagttac tgtagaagga gatgtgttta 7021aatagtatat cactctgtgg ctgggcgcag tggctcacac ctgtaatccc agcagtttgg 7081gaggccgagg caggaggatc acatgaggcc aggagtttga gaccatcctg gccaatatgg 7141tgaaaccccg tctctactaa aaatacaaaa aattagccgg gtgtggtggc acgggcctgt 7201aatttacttg ggtggctaag gcaggagaat tgcttgaact ggggaggcag aggttgcagt 7261gagccaagat cgcaccactg cactccagcc tgggtgacag agcaagaccc tgtcttagaa 7321taaatacata ataaatagta tgtattctgg cactttcgat acaaggaatt catggcttgg 7381ttgtatggtc ccaagaacat atcaatcctg tgttaatata agaatattat cttgtcctct 7441agataagcta ccttaccttc caggctcaca aaccacaagt atgtagccat actgaggcat 7501ggaacagaaa actgtatttg ttttgaatga gaacacattt gcctttatca gRccacagct 7561gccttccagg gtaaagtcag gcactgactg ttagcatggc cctgaaaggc aagggaacct 7621tcacatacct gacatttggt tttagctgtg gcccgaagca gtagttctca actgggggtg 7681gttttgtacc ctctctcccc aggggacatt tggcaatgtg tagatatttt tggttgtcac 7741atctagggga agtggtcctg ctggcgtcta gttagtagaa gcaagagtgc tgctgaattt 7801tctacaatgc acaggacaac tcccacagta aatatttggc tcaatatgtc actagtgcca 7861aagctgagaa agcctggcct agagtgatgg gtcttcgtgg ttgaaactaa aagaagaaat 7921tttcctgtat agtaaaaatg gatttttatt ttagctttta aaaataaaaa aggaataatt 7981agaatataat gaacactcaa atatccacaa ttagtcattg ttaatatttt attatattta 8041agacttggga tttaattggt ttcttcaaat aaaacgttta gttaatattt tctgtagtct 8101ctagaatcat atatttaaaa gaccaaatgt aggttgtcat tatgttatat ataattatag 8161ctttgtaggt atatgcattc tccccgctct gttcaggttt tcaagggtaa ggtttagttc 8221gtcataagca tttattgagt gcatactatg tgccaggtgc tgtttatatg gctgggtgtg 8281caatgatgaa taaaacacag tccctgcctt caaggacctt acagactggt gagaatgtct 8341gggagacagt gtgatcaaat gcctctgtag atgggtcttg cagtgagctt atcagggtgc 8401tgctgtgatt agaggtgggg agccttggat tcttgagtaa gtctcactgt gacatcagct 8461aagatggact tttatgtgct tcctttgaag gcatcacgtt gggtggtttc catttagacc 8521gaggccggtt cagaacttcc caaatgatgg tcctcctcct gacgttgtaa atcaggaccc 8581caacaataac ttacaggtat ggagcctccc acgaagccca ggcgagcttg acgtgatatg 8641ccaggctctc caatcctcaa cctttagatt gccagcttgc ggtttaccat tttattgggc 8701taaatatttc atgatttgtt cacatttctt cttagagcag cagctatttt taaaacgtcg 8761aatgtgccat cacattctat cacatatttt tgacgtggca atttgcattt tggcttaagt 8821aaataacatt tttttaaacc cactattttg agcgttcagt ggtctgtaac agtgtgttat 8881accataagaa ctggtatgaa gtggttaact actagtttaa taatagttga agcctgggcg 8941tggtggctca cgcctgtaat cccagcgctt tgggaggctg aggcaggtag atcacctgag 9001gtcaggagtt taagaccagc ctggccaaca tggtgacacc ccgtctctac taaaattaca 9061aaaattagtt gggcgtgcct gtattctcag ctgctaggga ggctgaggcg gaaggatggc 9121ttgaccttgg gaggtggagg ttgcagtaag ccaagatcac accactgcac tccagcctgg 9181gtgacagagc Ragactctgt ctcaaaaata ataataatag ttgcagatct agttgtttca 9241tttgatattt gctgccagga gcagtcaaaa ctatgacaac atcaacacag ttgtgctgtg 9301gaagcctgag aaacagccct atccagccta gggcatcatt ccctggggtc ctggctgcct 9361gctgctgtgc ctatggggca gggggcaggg atttaccctg ccctttccta acattatttg 9421gtgttcatca tagccctaat tgttttctca ttgtttcatt acctcattgt ttcattacct 9481gtaggaaggc actgatcctg aaactgaaga ccccaaccac ctccctccag acagggatgt 9541actagatggc gagcagacca gcccctcctt tatgagcaca gcatggcttg tcttcaagac 9601tttctttgcc tctcttcttc cagaaggccc cccagccatc gcaaactgat ggtgtttgtg 9661ctgtagctgt tggaggcttt gacaggaatg gactggatca cctgactcca gctagattgc 9721ctctcctgga catggcaatg atgagttttt aaaaaacagt gtggatgatg atatgctttt 9781gtgagcaagc aaaagcagaa acgtgaagcc gtgatacaaa ttggtgaaca aaaaatgccc 9841aaggcttctc atgtctttat tctgaagagc tttaatatat actctatgta gtttaataag 9901cactgtacgt agaaggcctt aggtgttgca tgtctatgct tgaggaactt ttccaaatgt 9961gtgtgtctgc atgtgtgttt gtacatagaa gtcatagatg cagaagtggt tctgctggta 10021cgatttgatt cctgttggaa tgtttaaatt acactaagtg tactacttta tataatcaat 10081gaaattgcta gacatgtttt agcaggactt ttctaggaaa gacttaYgta taattgcttt 10141ttaaaatgca gtgctttact ttaaactaag gggaactttg cggaggtgaa aacctttgct 10201gggttttctg ttcaataaag ttttactatg aatgaccctg gcagagactc ctgtcatcct 10261agcagtttac tctgcgtttg ttgtatctag acagtcaaca actgagttgt cggtgtttaa 10321cctgaatgct tgtttttcag aagaRgactg tttgtgccgg taagaatgat caggtaaggc 10381catgaaagtt tttgttggyg tttttgtttt tgagatgggg tcttgctttg ttccttgggc 10441cagagtacat tggctactca caagtgggct ggtagctggc tacagcccca gactcctggc 10501ttaagccacc tcctgcctca gccaccctgg cagctgggac tacaggcatg cgccatcaca 10561cttagcttga aagttttaat ttactaagaa tatacctgtg tttcccccca tttcctgatt 10621taaacagtac tggcttatat aggaacccat caaagttaaa ttccccaaat ttaaatttag 10681taaatttagt ggtttcacct tggcaaatct gcaatagttt caccagctca aatttcatgc 10741ttttgtaagc tgagcttatg tttgtgattt taatccttta agtactactg tggtaactaa 10801tcattttttt gttttttttg gtggtgtttt tgtttttgtt ttgagatgga gtctcactct 10861gttgcccagg ctggagtgcc gtggcgcgat ctcggctcac tgcaacctct acctcctgag 10921ttcaagcgat tctcctgcct cagcctcccg aatagctggg attacaggtg cccaccacaa 10981tgtctggcta attttttgta tttttagtag agacgaggtt tcacccatgt tggccaggct 11041ggtctcaaac ccctgacctc aggtgatctg cccgcctcgg cctcccaaag tgctgggatt 11101acagatgtga gccaccgcac ccagccaggg actaatcttt aaagcaaagt tttatatatt 11161tttacgtgag taatgttatg tgtaggtgtt ctatttggca aaataaatca gccttttcta 11221tcatgattgt ggtcatttaa attaatcctc atcggaaaca ttgtttacct aagataaaca 11281ttcactaaac aactcatagc aagacgctgg gtaggtacaa agatgtctta tgtgtggtta 11341tgctttcaaa gccaactgaa acccttttgt aaggagttaa atagcaaaaa gtttcaaata 11401actgtgtgcc tctagaacag aactatcaga aggcaatgaa tattcacaaa ttgtgaagag 11461atagtttgct tcaaggaaga aatgacttgt aactagagta ggtatgaaat gatgggggaa 11521aaatgatgag gtatgaaatg actcaccttc tctccagctc aaggtaagaa gggtggcagc 11581aggagtaaaa gctcagccac aggacgtgct cttcctgcca aacagctgga ttctgcacat 11641gcttgggaat gaggtcctgc tggtttagga aaacaaacat taagaattcc caaagtcttg 11701ggtggaaaag cctgttgctt ttcagaggca aggccatcac catttggcaa ggggcccaag 11761gccccaggca gctgtggtac catctgtttc tgaaggagtg gggattttac cccctgaaat 11821gtcagtttgt ggcttaaact ctgggttcta caggcccaaa ataaatagtg ccaggaaggt 11881gcccctgaac agagctaacc agggttcaga ttggcacccc atccccactg gggcaagcag 11941gactcttagc aattaaggcc taagacggct gacaggtggg acatggcaga aattcagtgc 12001ttccaccaca gctactcaat gtgccacRat catgggccac acaccactct gaatacttag 12061aaaattattg gtttgggcct ttattattaa gtagcaagta aataactgaa aaagccccag 12121atttcagctc tggctatagc agctttctaa gtctgtgttt ttaaggtgca atttcttgaa 12181tcctagttct gtagaagaaa cactctaccc ccccgccccc cccacccccc ccccccaccc 12241ctaaaaaatg agcttcatga tcaagtacat tttggaaccc tcaggctgat ccaagttcat 12301agatttcttc actgcaggcc ttctgggtcc tttaatatgc ctgaagctgg ggctttccct 12361tcctgagaat ggcccaggac tatccatgtg cctcattttg ggaaatttag taatgagtta 12421ctaaatcaac accacctttg gcattctaca tagcaaacat actttgttag atgtgacatt 12481ttacacacta accaatggag ataaaatttt ttaaatttat ttttggccag gcacggtggc 12541tcatgcctgt aataccagca ctttgggagg ccgaggcagg cggatcactt gaggtcaaga 12601gtttgagacc agcctggcca acgtggcgaa atcctttctc tactaaaaat acaaaaatta 12661gctgggcgtc gtcgtgggca cctgtaatcc cagctactcg ggaggctgag ggaggagaat 12721tgcttgaacc cgggaggggg aggttgcagt gagccaagac cacaccactg cactccagcc 12781tgggcgtcag agtgagattc tgtctcaaaa aataaataaa tgatttctta tttatttttt 12841tttaccagtg ctctacatgt tcagagaaac ttctctggta accaaccaca gaaatgatcc 12901ctgaaagtat agtcttagga gataaatatt ctttacatag ccaggaggga gatgagaagc 12961catacctgtc tacagtctga ctgacaaagg aggaggctcc aggtcccctg gagaatccca 13021ggttttatgt aataattact tggtcctttt caaagtactt ggatgagtgt aggatagtct 13081ctttgtctta tcagctagtc ctgcaatcta ggtgttctcc cattttgtag aggcttgcag 13141agatgagaga cctgtccaag gtctcaaacc cctaactggc agttctgaga ctagaaatac 13201tccctctcag gagtggacct cctctacaat tttttttagc agtcctcatg tgccaaatat 13261ggcgtggggg ccaggagaca gaatgaggca taggttgggc gtggcccctc ccctcttggg 13321tcttgtagtc ggctgtgggt ggcaagtaca gcagYgatgg cagacaggtg tgatgagttc 13381aaggacaggg aaataacatc cacagcccca tcccagtact cctgtgcagc aatggaagtg 13441tgccaccgac acaggggcta cagggtgagc aggagtgaca ggcgaagaga ggaagagagc 13501tctgcctgtg caaaggcgga gggaacatgg tgtgggagga actgaagaga attccacata 13561agtatgaaga gggtagggag attgagactg gagaaaaggg gaagccgggg ctcccctttt 13621gaccatgcaa cgcactggaa gcacagcact cccagcctca gggtcctcca tgattggaga 13681ctgacaRtga attcagagga gcttgtggaa gtcacccagt caggtccaga aatcctgcag 13741tgcatccctt ccgttacccc tgtcataact gggagaagac accacccaca tgacgcaggg 13801gagagtgcay gcatgagcta gcacccatat tgtcccctgc atctgtcctt ggctatgttt 13861tacacccccc accccttaga acattgtagt caaactagga tgtattaaat tggctggttg 13921ctcccaacta cactggacaa agtggagaaa aaaaaaaaag gaggagctca aggtttcaaa 13981ttcccagctc aagctctgca taaaaactgc tgtgacttcc ttagaagaga cccctatctc 14041ctgtagctgc agggttgaga tttctgagct caagtgatct gcctgcctca ccctcccaaa 14101gtgctgggat tacagttgtg agccaccctg cctggccagg gctgagattt gtgaaaagga 14161aacccaaagt ctcatcttgc atgtggctga attatgtcgc aaatccaatt cccaaattcc 14221agccttgcag ggtgtcttca gttaaagtga ggacaacttg aaaatcggaa tgggaacata 14281agggaagatt ccagtaaagc tggggacatt gaatccctaa attctgtYat gttccctgcc 14341agtagaaaca gcccatctac ccctccctgg gtccagctgt gtggacacag attggcagcg 14401agtgtgcagg tgttatttcc ctacccttgt aatcatcaca ctttcttttt ctttttcttt 14461cttatttttt tgagtcagag tcttgtgctg ctgcccaggc tggagtacag tggtgcaatc 14521tcagctcact gcaacctccg cctcctgggt tcaagcaatt ctcctgcctc agcctcccga 14581gtagctggga ctacaggcgc gcaccaccac acccagctaa tttttttgta tttttagtag 14641agacagggtt tcacccccaa tagccaggtt ggtctcgaac tcctgacctc aagagattca 14701cccacctcgg cctcccaaag tgctgggatt acaggcgtaa gccacYgtgc ctgacctcat 14761cacaatttct tagccctttg agaaaccctg tggccttccc tgaggtagct gtcttgcggg 14821gcacttctga ttcttcttgg atcctatgcc cttcccctct ttgctggacc tgtaactaga 14881ctcaagtccc accaggcccc agagagaaag gtacacactg tgacccatga agaggtgtgt 14941tacacaccaa aagaactgca tgatttttcc aatttataca gacaaatcca agagaYatgt 15001atgggagtag attttaaggt tgtgagatca cggaaggacc atagtgttgg atctggctga 15061acgtaatgat atgggcccac taagcagaga ttcttaactc agtgctttag catgaggggt 15121tagaaagggc tctaacttgg ttggttggtt gtttggttcg ttggttggtt ggctggctga 15181aacatggacc aaaaggtagg tggccgacac cgttccttat tttgttatta atatatttgt 15241atattcatat acaaacttgt atgctatgat atctaataca tattacagat ataaaacaaa 15301tctttaggcc aggcatggtg gctcactcct gtaatcccag cacttcggga ggctgaagca 15361ggtggatcac gaggtcagga gttcaagacc agcctggcca acagagtgaa atcccatatc 15421tactaaaaat acaaaaatta gccggtcacg gtgtcaggca cctgtaatcc cagctactca 15481agaggctgag gcaggagaat tgcttgaacc cggaaggtgg aggttgcagt gagctgagac 15541cccatcactg cactccagcc tgggtgacag agcaagactc cgtctcaaaa aaaaaaaaaa 15601tcttttgttt ctttcctctc tcattccttt atcacctaac ataagatgga tcatcacagc 15661attgaagtga caggatgtca agcagaagag taaacatcac ccaaggacgt tgcagcctct 15721ctggggaaag gattggcatg ttttctgttg tatgcaggat ggttgagtca tgttaggtgg 15781aagtatggct ttgttactgt ctttatctgg agattaagtg tggtttaagg agatgcatac 15841aggtgccagg ttgacaaaga gagtactgta atgattagtt ttatgtgtga acttggctag 15901gcccagttat gtaaacacga atctaggccg ggctttggtg gctcacgcct gtaatcccag 15961caatttggga ggctgaggca ggcggatcac ctgaggtcag gagtttgaga ccagcctggc 16021aaacatggtg aaaccccatc tctaccaaaa atacaaaaat tagcctggcg tggtggtgtg 16081tgcctgtagt ctcagctact cgggaggctg aggcaggaga atcgcttgaa cctgggaggt 16141ggatgctgca gtgagtggag atcatgccac tgcactccag cctgggcaac agagtgagac 16201tgtatcgcaa aaaaacataa aagaaaaaac aacaaaaacc ccgtgaatct aggtgttact 16261atgaaggtat tttgcagatg tgattaacct gcatgggcct catctaatta tctgaaagac 16321cttaattgca aaactcaggg ttccccaagg acagagcaat taagtctatg gcctgcagcc 16381tcagcttccg cctgagtttc cagcccacca tcctacccta cagcttcaga cgcgtctagc 16441cagcccctac aactgtgtag gacaatttct agaagttagt taatgaatac gctattggat 16501ctgtttctct ggaggaaccc tgactgacac aggggccggc tctggaggtt gtggggtcca 16561gttccttatc cctgtgtagt gtattggcca gttggggtgg gaattgcttc caggcaggct 16621gtgtcatttg ttcaggcagg gtcccctccc cttggctctg cctgagagca aaggcaggtg 16681ggggtggccc tggggagggc ctgaagcttt gctctgtgcc tcctaaagct ttcccactcc 16741agggaccagg ccaggcaggc tggcaggaag aggcaggctt catctggaag gatgtctcca 16801gggtggaaat gatgagattt aaatggtgta gggacccgga gggtggggtg ggacagcacc 16861actgacccag ctgcagagtt ggaccctctg aagcccattg tgtacatgag aatctgtgtg 16921cctgattgcg taggtgccta tacatggata ggctgcgaat ctgtgtgcac acagctggct 16981ggagagcaca tgagtaggta tatgtaccac attgtcagca ctcacccatt agctcctggg 17041gactcacttg ctgcaggtga tccctgtcct cgtgggctct acaccctggc aggtcaacac 17101agactacaaa gaaacaagcg gacataaaat gacaagggct aggacaaaaa gcatggaaag 17161gagaggggat aacgtggcag gggacatttt agaatgcagt ggtttcgaca ccaggaggag 17221gagtggttag acagtggtcg gggagggcct ctgaagaggt gacacctgaa tgatgagatg 17281gagcaaacca agcaaagatt ggaggaagag cctccaagca gaaagaactg caaggacaaa 17341ggccctgggg caggagcggg tacgtgtgtc ctgtatgtgt gtgtcctgca cggtggcagg 17401tgtctgaagc tgagcccaga ggagctgtgg agatcaggcc cagaagtgga gccacagcac 17461gtagggccag agacctgcag taagaaatgg gatgtggtcg agatgggatg ggagccaccg 17521cagcaccatg gggcgggagc agcagagtct gatgttgtgt acttcaggga gctggagttc 17581tatgaaggaa gagcgaggag gcatgtggga ggaagaacag ccccactgag gcctgcaggg 17641aaggcagcag accccaggtc agagcatgaa ggtagagggg aggttccaag aagtgggttg 17701gggaataagc actggatggc tgatgacaga gggcagggtg gaggatgcgg ggccagggag 17761ggctgcacag agtcctggga gggtgagcgg tggccctggc tggctaggag gcgccacctt 17821gaggtgctac tggaggatac gccgcaggga aggtggatgg tctcttctga tgacaacctt 17881cttgctggca ttagactgaa aggggaggtg atttgtaggg atgaggccag aggggttgtc 17941cccagggaag ggcaccagat gtggggcagg agagctggtg tgagttgcag ccgtggggcc 18001tagaggaagt cacagcaccc cactgaattc tcacttcctc atcataacag accctgtgac 18061atataaaacc tgctctgccc accgcacagg gatcacaagg ctggagtgaa ataatggagc 18121acttccccag agtggcacac gggacccagg gacgggtgtg caaaaccgtt ctaggtggaa 18181gaacactttt tttttctttt tctttttctt tcttttcctt tttttttttt ttttttttga 18241gatggagtct ccgtctgttg cccaggctgg agtgcagtgg tgcaatcttg gcccactgta 18301gtccctggct cccaggttca agtaattctc ctgcctcagc cttctgagta gctgggacta 18361caggcaccca ccacaacatc tagctaattt ttgtattttt agtagagaca gggttttacc 18421atgttgacca gactggtctt gaactcctga cgtcaaggga tccatctgcc ttggcctacc 18481aaagtagctg ggattacaag catgagccac cgtgcaaagc caaaaacact tgattataat 18541agcatgctat ttttcaatgt attagataaa tgtattttcc atttaaagtg gggatataaa 18601gctccccttt aaaatccaca taagtaaaaa agtcagtctg tttaaaaggt taagtaaata 18661acagtctagg gcagatgcac aaaagacagc atcatgaccg tggttcctga aggattaagg 18721ttgcaggaaa cactgaatgc taaaagcttt gtgaatgtat agtccatgaa aatgtctttt 18781aaaaaatgag gtgcatacca agatgtttga gtgtgtgtat ttggggtagg tgctgtgggc 18841atacgggtgt acctgtgtgt gtctcacaag caggtgtagc tgggtgcttg tgtgtatgtg 18901gtgtgtgtgt atctgtgcac acgtgcatat ctgtgcccac atatccatgc acacgtcctc 18961ataaggctct ctgcggccag gtgcggtggc tcacgcctgt aatcccaaca ctttgggagg 19021ccgaggcggg gggatcacga agtctgaagt tcgagaccag cctggccaag atggtgaaac 19081cccatctcta ctgaaaatac aaaaaattaa gacagtgagt gttgatcgtg aagaagggaa 19141gatgaatgtg gggattaggt gggaggaatg gggtgctgtc aagatgaaaa taaacttagg 19201gtggagaggc tggggcaaaa gagaggccca acagtatgat ttgccccagt gcggggactt 19261tgtgcctggt gatttgaggt ggcagggctt ctacccaggc ctgatgggag ctgggattaa 19321aagcagctgt gacctaaggc aggccattgc tctgtggcct Rctcccctaa ctcttttgca 19381gaaagtccca ggctaagaga tgggggatgg ttgtaatgac ccctcccaga aatcctggct 19441ctgtcctgca gaggtggggc ccggggatca agacgcctct aggttcaaga gagaggcaga 19501atgaggggtc agccagctgg ggcaggaagg cttcactcta ggctgcaagg agagaccttt 19561cgccttgact ggaaggacgt gaggagcctg tggcctcctg tttgcaacca agggctgagg 19621aaggggccgg gcattcctgc tgtaggtcaa tgacctggag aaacacatgc ttgaggacag 19681agctcagatc attgtcccag cgcaaacctt ggtgggatgt aaggtttgga gcttcttaag 19741ttccaagagg cagtctcatg cagttcaagg agggctggag aagcctggtc tgctcatggg 19801agtcaattgt taaattttca ggaattttgt gagccaatta ttgaacacag ctattattaa 19861aaacaaaatt ataggctggg cgtgatggct catgtctgta atcccagcac tttgggaggc 19921caaggcggtg gatcacttga ggtcaggagt tcaagaccag cctagccaac atagcaaaat 19981cctgtctcta ctaaaaatac aaaaacatta gtcgggtgtg gtggctcaca tctgtaactc 20041cagctacttg tgaggctgag gcatgagaat tgcttgagcc caggaggcag aggttgcaat 20101gggctgagac cacgccattg cactccagcc tgggcaacag agggagattg tgtctttcaa 20161aataataaaa taaaataaaa taaaataata ggctgggtgc cgtgactcac gcttgtaatc 20221tcagcacttt gggagaccaa ggctggaaga tcgcttgagc ccagaagttg gagatcagcc 20281tggggaacac agggagaccc tggtctgtac aaaaaataaa aacaaaatta gccaggcatg 20341gtggcacatc cctgtggtcc cagttactta ggaggctgag gtgaacaaag ttaacactca 20401aaactcatca cttgtgaatt actttgctac agtgcactat tattattatt tatttatttt 20461tactgggcag cctcttgagc ctaagtaggc ccagagactg tccagcatac tattattgat 20521gctttgtggt tattgatatt gattggatcc gtatagtgga aatggctata ctgtagaatt 20581gtgtgctact gtgtatttct taccaattct gcattcagtg gcatcacatt catagcttga 20641agttggccat ggtgggaggt aaatatgtgt ggtataaatt ggcaaagact acagattggg 20701gttcctgtct ggccccccaa gagctggttg ttaaactttt accagcacac cactgagttg 20761gagtctggaa agcaggcttc gtccaggttc tgccctgcta gctcaactgg ctctcaaagc 20821tttgctccac caactggcct atggctgctt ccactcacca attctccctt ttgccctcta 20881aggccacctc ttccaggaag tttcccctga tttctttgcc tggacttcta aaattctttc 20941tttgccaggc gtggtggctc acacctgtaa tcccagcact ttgggaggac gaggtgggca 21001gatcacttga ggtcaggaga tagagaccag cctggccaac acggcgaaac cccgtctcta 21061ctaaaaatac aaagattagc tggacatggt gacgcgcacc tgtaatccca gctactcagg 21121tggctgaggc aggagaatca cttgaacccg ggaggcagag gttgcagtga gccaagatca 21181caccattgca ctccagcctg ggcaacagag ggagactcca tctcaaaata aataaataat 21241taattctttc ttacctcttc cgttcatatt catacagaat aatctccaca tctcgaaatt 21301cttaactgat tagctagttc ttgctattaa ttaaatacgt gatgcatatg gccaagcttg 21361gatggatgga atccctgtat tggtaaggtg ggaatttttc ttttcttttt tttttttttg 21421agaccaagtc tcactctgtc acccatgctg gagtgcaatg gtgtgatctt ggctcaccac 21481aacctctgcc tcccaggttc aagccattct cctgcctcag ccttcctagt agctgggatt 21541acaggcacac accaccaagc ccagctaatt tttgtatttt tagtagtgac agggttttgc 21601catgttcgcc aggctggtct ggaactcctg gcctcaagtg atctgcccac ctcagcctcc 21661caaagtgctg ggatcacagg tgtgagccac cacgcccacc tagggtggga attttccacc 21721tgggttctgg gctcagctct gccacagatt cctctctctg ggcttatcct ctgaRggcga 21781gattttggca tgactgagtg gatggaggaa gaacttgggg tcagcgggct gtcaccctgc 21841tctgccgtgg aaccgactca acaacagggc cacaacaggc cttgttggac atggaagcaa 21901atgagaaaat tagtaatact gattcagtgt ttacttaagg ttctgatact ttgtccatta 21961tagacctttt gcattaattt tgatttttta ttatataaaa tatatatata acataaaatt 22021gatcatttta gccattcgaa gtgtacagtt ttgtggcgtt taatacattc acattattat 22081acaacccatc tccagaactc ttttcacctt gaaaaactga aactctattt ccattaaata 22141tgaactcact ccccgttctc tcttcctcca gcctctggtg acctccattc tattattatt 22201attattatta ttattattag agagagtgtc cctctgttgt ccatgctgga gtgcagtgat 22261gcaatcacag ctcactgtag cctcaaactc ctgggctcaa gggatcttcc cacttcagcc 22321tcctgagtag ctgagattat aggcatacgc caccacactc tgctaattca cttatttttt 22381ggaaagatgg ggtcttgctg catggtccaa gccagactta cattcctggg ctcaagcgat 22441cctcccacct caacctccca aaacattgag attacaggtc tgagccaccg caccctgccc 22501attctacttc ctggctctta aatatgacta ctctagatac ctcatataag tggaatcata 22561cagtatttgt ctttgtaact ggttcatttt acttggcata atgtcctgaa ggttcatcct 22621catcgtagtg tgtgtcagaa tgcccttcct tttaaaggat gaataatatt cccttgtgtg 22681gatatatcac atgttgttta tccactcatc attgatggat gcctgtgttg cttccacttt 22741tggctgctat ggaaaatgct gctatgaata cgggtgtgca aatattcatt tgagtccata 22801attttgattg ttttttcttt ttgagacaga gtctcacact gctgtccagg ctacagttca 22861gtggcgcaat cttggctcat tgcaacctcc acttcccggg ttgaagtcat tctcctgcct 22921tagcctcccg agtagctggg attacaggta tccgccatca cacctggcca atttttgtat 22981ttttagtaga gacagggttt caccatgttg gagactggtc tcaaattcct ggcctcaaat 23041gatccaccca cctcggtctc ccaaagtgct gggattacag tgagccacca tgccgagtcc 23101ataattttga tttttaaatg gtggctacta agtttttctg gcacccctta aattttgtca 23161ggcacttgcc ttaccctagt cctggcccca gtttgggttg tcacagcaag gtggaagtgt 23221actgaaaagc tcgtgtttta tctttatacR tttacatggg gccccaaaaa gggggaaaac 23281ccactttgat agggatatga cacagggacg caggagccaa ccgaaagagc ccccagtggc 23341caaagatsga gcaacttgag caacaaaata aataaagtaa gcatcaaatt atagcccaaa 23401gtataaaata agtgtctatg agtctatact cacatagatg atttaataca ttcatattaa 23461taaatgggag agcactttga gaggccaagg tgggcagatc acttgagccc aggagtctga 23521gaccagatta ggcactgtga tgaaaccctg tctctaccaa aaacacaaaa attagctggg 23581catggtgggg catgcctata gttccagtta cttgggaggc tggggaggga ggattgcttg 23641agcctgggag acggaggttg cagtgagctg tgatctcacc actgcactcc agcctgggca 23701acagagtgag gccctgtctc aaaataaata aatatataaa taagataaat atataaataa 23761aaataaaaat aaacgggaaa gaagagacaa atcttctgtg caggagaatY ccaaatgaat 23821tctgtagatt ctccacccta caggagggca cacacaactc caggctgcct tagtgacctt 23881cttccaaaga gtacagtacg ggaaggggga gcggggagaa ttaacttcac agtggagaaa 23941tctgacaaat acgacctccg ccaagtgatc gaggtcacat cagctgtcat gaattatgtt 24001gatagtgggc atcctggata tgatgggatg aaatggcact ttacctccac gacccttttc 24061acaacagccc gtaatgccag tataaggaaa acatcagacc aggagtggtg gctcacgctt 24121gtaatccgag cactttggga ggccaaggtg ggtggatcac aggtcaggag tttgagacca 24181gcctggacaa catggtgaaa tcctgtctct actaaaaata cacaaactag ccaggcacgg 24241tggcgggtgc ctgtaatccc agctacttgg gaggctgagg cagaagaacc gcttgaaccc 24301gggaggcgga ggttgcagtg agctgagatc gcgccactgc actccagcca ggttgacaga 24361gtgagactcc atctcaaaaa aaaaaaaaaa aaaagaaaag aaaagaaaaa gagaaaagaa 24421aaacatcaga cagatcccaa cagaagggca tcctacagta tacRtgacca ctgctcctca 24481aacctgtcaa gactatcaga aacaagagaa actgtcacag ctacaaggag atgtgacaat 24541taattgtgat tttttttttt gagatgtggt ctcgctttgt cacccaggct gcagtacagt 24601ggcacaatca cagctcactg cagcctcgaa ctctggggct caagtgatcc tcccatccat 24661ctcagcctcc caagtagctg gggctatatg cgtgcaccac cacacccagc taatttttgt 24721atttttttag agacggtatt tcgccatgtt gcccaggctg gtctcgaact cctggattca 24781agcaatccac ctaacttggc ctcccaaagt gctgggatta caggcatgag ccaccatgcc 24841ttgccaaatg tgatgtattc ttgatgggat cctggaagag gaaaaagata ttaggtaaaa 24901actaaggaca tctgaataac catggatttc agtaatgtat ccacactgat tcattaattg 24961taacaaatat accgtatgaa tgcaagatgt tcataatgag gccggggcgg tggctcatgc 25021ctgtaattcc agcactttgg gaggctgagg cggaaggatc tcttgagctc aggagttcga 25081gaccagcctg ggtaacacag tgagatcccc atctctacaa aaaatttttt gaaattagcc 25141aggtgtggtg gtggcacatg cctgtagccc cagctactca gaagactgag acaggaggat 25201tgcttgagcc gggaggttga ggctgcagta agccaggatc acgccactac actgcagtct 25261gagcaacaga gcgacaccct gtctcaatga taataataac aataataata ataatggaaa 25321ctcagtatgg gttgtatatg ggaaacttgc tcagtttctc tgaaaatcta aaattcttct 25381gaaaatcaaa gtctgcttaa aaattcacat ggggtttgca ttctcctcca tgacaaggtt 25441ttgcaggtta tgattacaga ggctggagag ggtgcaggtt atcccgcccc tccctgtccc 25501agggattccc ttccccagga gctgtgtctc ccctgtgaga gagggtgagc ttccatgacc 25561ccaagcctct tgccctctga ctccggtatt cttagaagct gggaccagca ctgagcccaa 25621attcccgaag cgctcaaata ctggctttct gtccctatgt gacctggagc ttgtagttta 25681acttctctct gcctcactgt ttgacctata aagcagggca atcaaggcat cccgggggtg 25741gctatgaaga gtgaatgaga tagcagacaa cccagatgcc taccgacagg tgaagggacc 25801aacacagtgc ggtataggcg tataagggaa tggagtatgg acacagccta caacacagac 25861aaaccttgaa gacattcctc taagggacat aggccaggca tggtggctca cacctgtaat 25921cctagcattc tgggaggccg aggcgggcag atcacttgag gtcaggagtt gagaccagcc 25981tggccaacat ggcaaaatcc cgcctctact aaaaatacaa acattagctg ggtgtggtgg 26041taggtacctg taatcctgga tactcgggag actgaggcaa gagagttgct ggaacccggg 26101aggtggaggt tgcagtgagc cgagattgtg ccactgcact tcagcctggg cgacagagcg 26161agactctgtc gaaagaaaga aagggaggaa ggaaggaaga aagaaaggga agggaaggga 26221agggagggga ggggagggga ggggagggaa gggagagaga aagaagaaag agaaagaaag 26281gaagaaagaa aaagaaggaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa 26341agaaagaaag aaagaaagaa agaaaagagt gaacccagga acgaaagatc acacacactg 26401tatgactcta tttacatgaa atgttcagag taggcaaatc catagagaca gaaagcacat 26461ttatggttgc caggagctgg gaaagggcag gatggggaat gactgtttat tggatgtggg 26521gctctatttt ggggtgatga gaatgttctg gaattaaatt catggctgca taacactgtg 26581aacatactaa atgcccctga attgtacact ttaaaatggt taaagtggca agttttcact 26641aagcagtaaa ttaaattcta ctacaatttt aaaaagacta aaaaataatt taaaaaagat 26701taaatgagat aacgcaaaaa agcattatct cgaaaataca gctgatatta gtataattct 26761tactaagttt taagagtcta aggtgcagga ttctaagttt aaagggatag gctcttttgg 26821ttttttggtt tagttatttg gttttttttt ttaatccatt atccccaccc ttgggaggcc 26881cccagcaccc agtctgcact agaggatggg gcccacctcc cttttctctc caggcccagc 26941cactgaccac cagtaccctg gccaggggca ccctcggtca ttgccctccg tggcccaagg 27001aagggaacag aaacaacagc caagaagaca atagccgccg ggaagtcctc acatttctgg 27061agaaatagag cccattaatg aatgaagttc ctccagcctg atcggaggac ggggtgctgg 27121ggaggcctgg gctaaagggc tcacctccag cccccaccct ggcagggccg atggtacatg 27181ctcactcagt gagggggctc cagaggtctg tgggtacgaa cccaagggct ggtgcccagg 27241ggcaatcagc ttatgtctct gagccttggg aaacagtgag ggtcagcccg gctccccacg 27301tgcttctggg cagctttggt attggagcag gtgcaaactc Rggactaggg caggaccccc 27361tgagaggcga ctgagcaagg ccatcccgac tcatgtttcc ttggccctgc ccggggcaca 27421gcatcctgcc cacatccctg cagccctggc tccttcctag gggctctgag gaggcagcac 27481ttggtcatct ggtcacagtt gctgcagggc agttcttggc cccagctgta ggtaaagtac 27541tgtatgttgt aattttttga aagataacac gttcacacaa ctcagaattg aaatgccaca 27601gacattcccc ctgctccgcc cctttccccc ggatacccag tttctcccgg aggcagccaa 27661tgatctcaga ggctgtatac ccccccagag ttattttatg catatcaagg aaagtctaca 27721tagaggactg tttctggggt acccagatgc agcgtcaaat gccatggaat actacagtga 27781ggacattatc ctttcaagct ttcaaatcag agcaagggaa aggtcgatgc tagagtttct 27841ctagcaccca tgaagccctc tccctttttc tactgagttt tactttacag gcaacagcag 27901gcttcaagct tggggtcatt gtcgggcaac agtatctggc aagaattcaa tgtctttttc 27961tcatagtcat tgtattttgg cctctttcta tttatggcaa ctgagagaga aagcttattc 28021ctagatatat gtatttaagt aaaaaataaa tgaattcatg gaaacatatt aagcaattat 28081ccagataaca taagggatgg caaaaatggt gcagatggtg gaggggagac aagtagaagt 28141tggggtgctc ttgttgaatg tctggctctg aactctagag gaggccgcag gggctgggca 28201ggaaggaggt gaatctctgg ggccaggaag accctgctgc ccggaagagc ctcatgttcc 28261gtgggggctg ggcggacata catatacggg ctccaggctg aacggctcgg gccacttaca 28321caccactgcc tgataaccat gctggctgcc acagtcctga ccctggccct gctgggcaat 28381gcccatgcct gctccaaagg cacctcgcac gaggcaggca tcgtgtgccg catcaccaag 28441cctgccctcc tggtgtgtaa gtatcagtgc atctgtctgc cctgccaggg gtcttttcat 28501ggacacccac tatgccagga gcctccctgg cctgaagcca gccctgaagc cggctgccac 28561actagcccag agagaggagt gccctgggag ggagatgggc tgagYggagc tgtcatcacc 28621ccctcctgac ctcgccttca aggtcaagtt ctttggtRag aaggtcctag ctgcattgca 28681aacagccagg tatagggatt tgtgtttgtc tgagacccag aatcactggg gttcRagtta 28741gggttcagat ctgagccagg ttagggggtt gagtcagggg gtaaagatta ggaggttggt 28801gtatatttgg tgttgggggt cactctatgg ccaaagtcag gggttgccat gagctcaggt 28861gacggaggct ccatcactga ctgttgtgtg actttggcca gctcccctgc cctctctggg 28921cctcagtctc ttgctcaata taataagggt atagggaggc taaatgatac aatttctaaa 28981atagagtatc gccaagttca aaagccagaa ttatagaccc ccaggactac agacagtgtc 29041acagcatcgt ctgggtgagg ctagggttag tgtgcggctg ggctcagggc tgccccattt 29101gctaggatcg tggggttccc atgtgtcagg atccagaggc tagggtatga tcaggatctc 29161tagctggggt cagggtcaga gctctctgtg tcccctagaa ttgccatcaa ccttaaaccc 29221agagggaggc ccagtccaac ccctcagctt taagacctgc tgggagcctc atctcagaga 29281ggctgagtca tggccaaggc cagttggggg tgggagcagg gggcttggtg tgggcctgca 29341gcccctcatc cactgccctc ccctctagtg aaccacgaga ctgccaaggt gatccagacc 29401gccttccagc gagccagcta cccagatatc acgggcgaga aggccatgat gctccttggc 29461caagtcaagt atgggttgca caagtgagtc gggcctcggg tgtgaccagg ctgggggtag 29521ggaggcggga ggaacagcct ggggcttgcc cccagcccac agggaggaaa ggcagcagct 29581gggggactca ggtctctccc cttgatttgg aaccagagcc tgacaccttc cctaccccca 29641ccctccatac cctggtgccc tggggggatt tattggagtR tatcaacctc tccaacagcc 29701cctctaagag tcaggcttca aagggtcctt tcccactgcc ctgggaagat ggaggtctta 29761tttcggggtg aatggggggt agggtagaaa aatctcaaca aaataagtat tttttaaaaa 29821aatgtataaa tgttgttttc ttatagtaca gacaggtctt gctatgttgg ccaggttggt 29881cttgaactct tggcttagcc agtcccccac cccaagccta aaattagtat cttgacttta 29941tttgggatga tggtaacagt caagggtcgg ttgtgggcca ggtgctttta caaacaaYat 30001cctcaccttt agcatatatt tttttctttc ttgtttttca ttttttgaga cagagtctcc 30061ctctgtcact taggctggag tgcagtggca caatctcagc tcgttgcaac ctccgtctct 30121gggttcaagg aattttcgtg cctcagcctc cagagtagct ggaattacag gcacacgtca 30181ccacatccgc gtaatttttg tatttttagt agagatgggg tttcatcatg ttggccagac 30241tggtctccaa ctcctgacct caagtgatcc gcccacctca gtctcccgaa gtgctggggt 30301tacaggcgtg agccaccacg cctggccctt tatctatctt aatctttatt ttatttattt 30361atttatttat ttatttattt atttatttat ttatttattt attgagatgg agtctctcac 30421tgtcacccag gctggagtgc agtggcacga tctcagctca ctgcaacctc cacctcccgg 30481gttcaagacg ttctcctgcc tcagcctccc tagtagctgg gattacaggt gcccaccacc 30541acgcccggct aatttttgta ttcttagtag agacggggtt tcaccatgtt gacctggttg 30601gtcttaaact cctgacctca agtgatccac ccgcctcggc ctcccaaagt cctgggattg 30661caggcgtgag ccacagcgtc cggcctatct taatctttat catatatttt agcctcttct 30721gtaaaagagg ataatgccat cgcccggatg accctgctta acaaacacgg aaacaggagc 30781cgatagaggt ttagcagcct ggcggagctc atgtgcaaac tcagctcagt atcggaaact 30841cagccacctt catggcacct caggctgccc tggagcctgg gtcccggtct gtgtgtcggt 30901ctgaggcccc aagggtgggg cagacattgt tcagggacct tgtctttgtc ttaaagccaa 30961tctcctcctc ctccccggcc ctgctaaaca cgctccccag gggtggtctc tgctacccgc 31021gcacattggg ccttgagtct tggtaaatgt ttctgagcca ccagggggcg ccagcggtgc 31081tggatgccac ttcgcctttt gcaggaaaac tcccgccgct agcgggtgct gtggggcgtc 31141ccaggtctcg cgcagccacg ccctctgtgt gaccttgggc ttcagtttag gcttctgtaa 31201aagagaataa tgggcaaggt ccagtggctc atgcctgtga tcccagcact ttgggaggcc 31261gaggtaggag gattacctca gccagggttc aagaccacct gtctctataa aaaatttaaa 31321aattagctgg gcatggtggt gcgtgcctgc agtcccagct actcaggagg ccaggggagg 31381tcaaggctgc ggtgagctat gattgcatca ctgcattcca gcctgggtga cagagcaaga 31441ctctgttgct aaaaaataaa agaggataat gctatcctag acctatgtgt tgcaagaggt 31501cagacatggc aaaagcgttg agaagttaaa gaaatagtat ccttaataaa tcagcaataa 31561ttcagtaaga ccagactgcc atagactaga tgtcatagtc attaatatat aactgttatc 31621ccaaacgtat atgagtgggg tagaaataaa atatgattgg ccataactat gattggtcat 31681gattaatgtt agatgaagtt ggataatggg taagatgaga attcttcaca ctattctctc 31741taaagcgaat ggaataaaat agaacagaac agaccgggaa agccagatat atgtggattc 31801tccttgctgg gcacagtgtg tcatagtggc ttataatatg ggatttggag tgggatggac 31861ccaggatcaa attcccttct actgaggttt tctgaagcca cataagctgt ctgagccatt 31921tctgtctcag ttcagtgggt acagcaatag tgactacctc gtgggtagct gtgaggatta 31981aaccaggaaa tgtgtataaa ctctaagcac agtgcctgcc acacagcatg tgtttggtac 32041gtgatggccg cttctattgt gtcatattcc atgtgctcaa gccgggccct gggggagagt 32101tcagcaaggg tagagggata tgagagtgaa tgtggcccac tggcctccct gcctgcagtc 32161tctcctctct gatccttgct tcctaagggt cttcactgag ccccacctct gaccaagtcc 32221ccggcggcac aggcagcaca gatcctctgc tgtacctaca ccaagacccc agggccaacg 32281cttctcccat tgtgcagctc cacctctgcc cccggggcct agctgaggcc tcctccaagc 32341tgagccacct actatcttcc aaactcccac cctccatctg gtgtcacctc ctcccagaag 32401ccctctgtat cagtttcctg ttttctgcca taaaaaaaat cactccaagt ttagtggctt 32461aacatgacac aagtttacca ttttacagtt taaaaggcta gaagtccacc atggccctcc 32521Ytgagctaag atcaagctgt cagcagaacc agattccttc tgggggctat aggggagaat 32581taattccctc gctttctgca gcttctagag gcttccccca ttccttggct tccctccatc 32641tacttttatt tagggcgagg gaagcttcct gctctgactt tctcatttat ggtattctcc 32701cctgctttcc agcagctgtg attgtcccaa gtcctgtcct ctggttctct aggccagaaa 32761aactatgatc taccagagct caagaacttg aaagggcctt ttcccactgt cccttctcac 32821agtgagagcc ggagcccagc ttcgtgctag aatctgtgaa aatggagaaa tgcaccctgt 32881gctgttctct tcctccaccc ggcatctccc ctgcagcccg gctgggtttt tttgtttgtt 32941tgtttgtggg tttttttgtt tttttgtttg tttttgagac agagttttgc acttgtcgcc 33001caggctggag tgcaatggca cgatctcgac tcactgcaac ttctgcctcc tgggttcaag 33061cgattctcct gcctcaacct cccgagcagc tggaaccaca agcatatgcc accactcccg 33121gctaatcttt tttttttttt tttatgcatt tttagtagag atggggtttc accatgctgg 33181ccaggctggt ctcgaactcc tgacctcaga tgatccacct gcctcggcct tccaaagtgc 33241tgggattata ggcgtgagcc acggcctggc ccagtctgac tgttttcact tactctctaa 33301tgtctcaggt agtgtttatt gtattctgtg cggaggtcaa gaatttagtc atccaaggag 33361gttagtgtat taggagctta cgcagctatt accagaagag actccactca aaaatttggt 33421acgtggctgg gcgcagtggc tcaaacctat aatcccagca ctttgggagg ccgaggcggg 33481cagatcacct gaggtcagga gttcaagacc agcctggcca acatggtgaa accctatctc 33541tattaaaagt acaaaattag ctgggcatga tggtgcatgc ccataatctc agctacttgg 33601gaggccaagg caggagaatc actgaaccca ggaggtggag actgcagtga gctgagattg 33661cccgctgcac tccagcctgg gcagcaagag caaaattctg tcacacaaac aaacaaacaa 33721acaaaaaaaa cgggttgggc atggtggctc atgcctgtaa ccccaacact ttgggaggct 33781gaggtggggg aatcacctga ggtcaggagt tcaagaccag cctggcaaca tggtgaaacc 33841ccctctctac taaaagtaca aaaattagct ggatgtcgtg gcgggagcct gtaatttcag 33901ctactaggga ggctgaggca ggagaatcac ttgaacccag gaggcagagg ttgcagtgag 33961ccgagatcgt gccactgcac tccagcctga gcaacagagc cagactcagt ctcaaaaaaa 34021aaaaaaaaaa caatggtatg agctttaaat ttctgtagaa ctgaaagagg attggagatt 34081gtagtagggt ctaattctaa attagagcat ttagaataat gtggagtctg gtcaacatag 34141tgagaccccc ccaatctgca caaaaaagac aaaaattagc caggcagtgt tggtgcatgc 34201ctgtggtccc agctattcca gaggctgagg caggaagatc gttggaattc gaggctgcag 34261tgaactgtga ttacacaact gcactctggc ctgggcaaca gagagccacc ctatctcaaa 34321caagaaagaa caaactaaaa aaaaaaaaag aagaagaaga atttggagca atctagttgt 34381ttcctatacc agaagtcttt gccaatctat ttttttttaa ttttattttt aatttttgtg 34441agtacatagt aggagtatat atttatgggg tacatgagag tacatagtag gagtatatat 34501ttatggggta catgagatgt tttgatatag acctgcaacg ttttgtcaat ctattttttt 34561tttggcgaca gtcttgctct gttgcccagg ctggagtgta atggcgcgat ctcagctcac 34621tgcaacttcc gcctcgtggg ctcaggctat tctcctgcct tagcctccaa actagctggg 34681actacagacg cccaccatca tgcccggtta atttttttgt atttttgtgg agacgagatt 34741tcaccatgtt ggccaggctg gtcatgaact cctgacctca ggtgatctgc cctcctcggc 34801ctcccaaagt gctaggatta caggtgtgag ccaccacgct ggcccaccaa cctatttttg 34861atctaacttt acctctggtg gtgtattgat actcttccga atcaattttc acccttcttc 34921atataccatt tacatttcta agaatttgtc catttcattt catctagtta acctcatttg 34981ttgacataca attgttcgta gtattctctc ataattcctt ttatttctgt aagactggta 35041ataatggcct tgcttttatt tctgttttta gtaatttgag tcttcttctt tttttttttt 35101tttgccagtc tagctaaagg tttgttgatt ttgttgatct tttggaaaaa ccagctattg 35161ttttcaccga ttgttgtttt tgttctatta tttcattcat aggcactaag atcctgatta 35221cttccttcct tctgctagct ttgggcttag tttgccctta tttttcaagt tccataaggt 35281ggaaagtttg attattgagt tgagatcttt ctctttttta aagataggca tttctagata 35341taaatctccc tgtgagcacg gttccctcca tcttcagcac accagggttg actctctccg 35401ggcgttcttc cctggtcacc tctccccttc ctctcctctt ctgcctcctc ttccactttt 35461cggtaccctg tgattgtatt gggaccaccc agataaccta ggatcatctc cccacctacc 35521ccaaggtcct taacttaacc atactttcat atgggtaaca cgagttgagt gtggtaccca 35581gggtttgaca tgttgggtaa catatttgca ggttctgtgg attaggagga cattttgggg 35641gccatgattc tatcttccac cctcgcctag acaaaattgg aggctcactc cttgggctcc 35701ctggatgacc cccaacatcc ttcctcactt ccattccttc ccagcatcca gatcagccac 35761ttgtccatcg ccagcagcca ggtggagctg gtggaagcca agtccattga tgtctccatt 35821cagaacgtgt ctgtggtctt caaggggacc ctgaagtatg gctacaccac tgcctggtgg 35881taagcattcc tgtcagctga tgccccatgc cctggccctc tctgggctgg agggctgaat 35941gagggtcctg ggtccttggc tctttccagg ctgggtattg atcagtccat tgacttcgag 36001atcgactctg ccattgacct ccagatcaac acacagctga gtatgtgtca agcgtcctct 36061ggggaagtgg gagctggact ccagggcttg gcctcagcag agggggaggt tgtgcaggca 36121gagggttctg gggccaccaa aggaggccca gcctgggaag tttgcagggK tggggacccc 36181agagctggcc aagctcttga ctggcctggg cagcatgtgg ataccatctg atagcggagg 36241ctgccctgag gtcatgtcgg gtctccctgc agcctgtgac tctggtagag tgcggaccga 36301tgcccctgac tgctacctgt ctttccataa gctgctcctg catctccaag gggagcgaga 36361gtaagtacac caccctgtgg cccccattcc tgctcgtgcc catcctgtta gtgtgtccac 36421ggctccYtcc aggctcaacc ccacacaggg catgcttgtg ggtggccaaa cctgagggca 36481gcaatacctt cagtggggtc acttcctacc ccctcccatc aatacaccct caaaggctgg 36541aaacaacaat aaccaacagc tagtaactaa cagctattaa gaacttgctg tgtgcaaagc 36601actattccaa gcccttttca tgaattaatt gattttgtcc ttaaaaccaa ccctaggata 36661tagattctgt tatcatcccc tttttacata tgggtaaact gagtcacaga gaggttagaa 36721aggaaaagct catatctacg gagtgcatcc tgcattccaa gcaccacact aactcagaga 36781taaaactcta gccaagctaa gtaacttgct gaggacacac aactcgccac taagggatgg 36841gagtaggatt tgaacccagc attctctgac cccagaagct gagttcctag atactttact 36901ctcctgcttc ccagggtggg gctttttgtc ttggccaaca ccctctgtca aggagctgtg 36961ggtaacccca ttgcacagag gaagataaca aggtttggag agtccctagt catgttacca 37021atgccaaacc tggaaggcag aagggaactg gtgggtgggg tctggagagg agccctctat 37081tcaggccatt ttctgactct ggagcagacg gatacatgta tgaatttgga ctctagacac 37141gttctcgtgt gtgtgacagg tgtgagcgtc acaggagctg ggccctcccg aggaattctg 37201ggatggtgcc acagttaatt cttgggtctg aggctccgtg ttctcagctg caaaatggga 37261gtgataattc ttacttcctg agctacaaga gtcagggcca acagagccat gaacggtgcc 37321tggtacacac taggcRctcc atggatgcac aggactggtc aggggctcat tgtggtgctt 37381gctgccttca ggcctgggtg gatcaagcag ctgttcacaa atttcatctc cttcaccctg 37441aagctggtcc tgaagggaca ggtgagtgag gctggctgac tccctgtggt ccagggccat 37501gcccaggagg ctggatccct ttcctccctg cctttccctg agaaggtgcc actcccacct 37561tctccatgtg gccagtcccc tgtgccggtc cccagcactg ccaccaccac gcagctggaa 37621ggaggcactg cctctggcct cctttcctgc ctggaaagca cctgctctgt ctgccccaga 37681tctgcaaaga gatcaacgtc atctctaaca tcatggcYga ttttgtccag acaagggctg 37741gtgagtgYgt ttctgtctgc atgcctcaga agacagcagt gggagccaga aagccacctg 37801ctgcactatg tggccttggg actgtcactc ttcctgtcta ggtcccatgg gctctatctg 37861gctctgacac ttgatgatta gttatgagca tactttggca aatctctgcc cctttgggct 37921gcagcMtcac aagctgtgtg gcgttgggca agtctataga actcaggaca aatgggtgat 37981taagtccaag aggactccaa gattctcctg gaagtagatt aggaaaaaag ataattagat 38041tgctcacatg gctgggcact catccatgta ctgtactctc ctatgcagta cagagcagag 38101ctgggtttca gcccaagtct tggactctgc tctgaaccaa ccttctagaa gggctctacc 38161tacccagaca gacagacttg ggaaaagaga gaatgaaaaa gtgccacacc cctccccgca 38221cacccaggtc ccactttaca gaggggaaca ctgaggctgg agggttgggt agctgtgtgg 38281atgcagggga gcggtgactc agggcaattc ccccatcctg aggccctgcg ttgatctttt 38341cctcctgcag ccagcatcct ttcagatgga gacattgggg tggacatttc cctgacaggt 38401gatcccgtca tcacagcctc ctacctggag tcccatcaca aggtaggagt tgtgggaggg 38461tggggcaggg cccagcttcc ccaggggagt tggtcctttt ttgtgctctg acaaccccgt 38521cccccagctt caaccttatg gcagccaaga gtcctggggc gctcctcctc attcctgatg 38581ctcctccgca ttcctgatgc tgcgaggagg gcaggccaca gcgacgtgcc cctgacccct 38641ctctgcaggc accagggctg cccactacaa ggatcccagc aaagcaccag ctccttccta 38701gagggcttat tcggcttctg tcatcctcta cagcagtgga ttgtggcccc cYcccagggg 38761gtactgacaa aagctttgga ccctctatta cttaggatat agatttctgt aacaaagaaa 38821tccgaaataa tagctgctta aacacaataa acagttattt ccctctgttg cgttctgagc 38881atctgcagcc ccgcgggatc aggaggctgc agggtgtcag gcacgcaggc cccttccacg 38941tgttctcagc cagccccagg catgacctca tcccagggtc ctggctgact ggcctgacca 39001ccacacccac tctgctcaca gcccgttggc ctgaacYtga tcgcaggacc ccaccctagc 39061tgcaagggaa actgaggaat ttatttcagc taaatattga gaccagctaa aagttaagtg 39121gtacagaatg gagggagaga ttggagaaga ctttagggcg gacccagcca tctttgtaac 39181agagcctctc cctggaaaac tggacagaag gatcatttca gaggttcatg gccactcctg 39241aagcctgttc acaacccatg ggctggatga tctagtgggg cgggggactg ggcagcagct 39301tgttttcctg attttggcct ccaggagccg atggtcaatg gactgccctc tgcaggggca 39361gggctggtgg tcagctgggg cggggtggga gctggaggtc cgtggtcacc agctgccctg 39421actaatgtcg ttacttgaat ataaccctgt gaaggcagga accacgtctg tctggttcac 39481ttcccacggt ggttgagaca tagtgggcac tccggaagta tttgttgaat gagtgaaagc 39541cccgctgggg gaaactgggt acagctcttt cctcagtttc cccatctgca ctctgggctg 39601aatgctgggg ctcctcccaa tctccctgaa gctggacctg agcccagtag ggacacacag 39661ggtccagcca gcgtcctggc ttcctccagg gtcatttcat ctacaagaat gtctcagagg 39721acctccccct ccccaccttc tcgcccacac tgctggggga ctcccgcatg ctgtacttct 39781ggttctctga gcgagtcttY cactcgctgg ccaaggtagc tttccaggat ggccgcctca 39841tgctcagcct gatgggagac gagttcaagg tgagtgggtg gggctgggct gctagggRat 39901ccagatggca tgtggtatgt gtgtgtgtgc acacgcatgg ggaggaggga ggaaactcgg 39961aaacttggtg gtgggcaaaa gaactaagct ggagcaatag cagtgaagtc cagactgggc 40021acagtggctc acacctgtaa tcccaatcct ttgggaggct gagatgtagc aggacgaacc 40081gcagacaaaa ctcctcagac actgagttaa agaaggaaag agtttattca gccgggagca 40141tgggtaagac tcctgtctca agagcggagc tctccgagtg agcaattcct gtccctttta 40201agggctcaca actctaaggg ggtctgcatg agagggtcgt gatctattga gcaagtagca 40261ggtacgtgac tgggggctgc atgcaccggt aatcagaacg aaacagaaca ggacagggat 40321ttttacaatg ctctttcatg caatgtctgg aatctataga taacataact ggttaggtca 40381ggggtccatc tttaactacc aggcttaggt caggtaggcc caggcctggt ttcgggtctg 40441gttccttggt ttcgggtctg gtctctaggc gttgggctac ctgcctttag tttcgcttct 40501ctttcttttt ctgagtataa aacaatataa aacaatatga gagggtctgt ctctcttctc 40561tcagagacag gagggtccct ggaggccagg agttcaagac cagcctgggc aacatgggga 40621gacccctgtc tctacaaaaa taaaaataag tttaaaaatc agctgtgcac ggcRgtgcat 40681acctatagtc ccaactactc aggtggctga ggagggagga tcacttgagc ccaagaggtt 40741gaggctgcag tgagctatga tggcaccact gcactccagt gtgggtgaca gagtaggact 40801ccatctcaag aaaaaaatac agtccagcat ttattggagt caactatgtg ttgccagata 40861gatagatgga tagatagaca gagtgatcaa tagatttagg tatagatata acttggcacg 40921tagtaactgt gcattaaata taagttgata ttagacttac atttattgca gttatatttg 40981ctacactttg ttttgttgca ctcatcctgt gaaataagta ctgttacctc tgttttgcaa 41041acaaaaaaac cacaaagctc agagaaggta ggtgacttac tgaagatcac acagcctgta 41101agaggtggcc ccaaagcctg tactcttcac ctatactgta ctagaaatgc ttaggtagtt 41161tccaggctgt gcttgtcatt gaactcatga ggaaactgag gcccacagag gggaagagac 41221ttgtgcgagg tcacacagca tgtgtggggc acagtgggga gcagaagcca ggcctccagc 41281cgggacaggg gttccctgtt ccacaccctg cccagagcat ctcacatgtt gtctgggagg 41341ttgggagttg cgtctgagga ggggtccagt ccttgaaact gcccttggtc cctgcgaagt 41401tttcttctga ggagtggact ttactccacc caccctccaa cttcctcatt tcttttcagg 41461cagtgctgga gacctggggc ttcaacacca accaggaaat cttccaagag gtaactgccc 41521cctgcccctg tgtggggttt atctcacgta ccccaatcct gctctggctt caagagcccc 41581cacacagcca ataacaccac caatggcaac aattataaca gcgaacacag ctcctactcg 41641gttgttcggc atggagtgaa gcacttagtg tgtgtgactt ccttcagtgc tcacaccgac 41701cctatgagtg gggcggtcaa actgtcccca ttttacacac agggaaactt agtgaatggc 41761aaggctgggt ttgagcccag ctctattgcc cccaaagata aggctccatt ccctgctcca 41821tttcccaggc atagRgactt gtagggggct ggaaccccag gatcaactct gggctcagag 41881ggccccagca ataagtgact gttgattact cctgatccca aagctgactt caggcaagct 41941ccttggaggt cgcagcccct tcttgctatg cccagtggca atgatgttca taatcccact 42001cctcagtgca gggttccact aagaacccat gatctcctac ctcaaatgga cctcatgctt 42061tctgagtaag cctccctcag ctttctggtc acctcactcc ccccacccac tgcaatgact 42121tcttcaggcc ttccctgcca tcctcaaatc tccagctgcc ccctcctgtc taccttccac 42181ttccctctcc acacacaacc tgcttaccag agagctgagc agagccacca acagaacttc 42241ccccccacgt cgctgctccc agtcgcaacc cactcaccca cacctgcgcc ttcctgcggc 42301cgttctcctg ttctaacaga ggacRgtccc tcttcccttc tgaactcagg ccgtccagcc 42361ttccccggct ctgtgatgYc tctctccYtg cacatacaca actcacaata ttgggcaggc 42421ccctgaccgg cYcttctccc ttagcccctt gtctgagtcc tgttcctttg atgtcctatt 42481tctagtcttt ccatttctct ctgtctctac ctcctcccca ggctaccatt atctctagtg 42541gggacccatc tgtagctccc ggtgggtctc cctgtgtcct gataccctcc aataatctgt 42601tccccacagc agccagaaca atcttttcaa gaaataaaac tggtcattca cctgcctgct 42661taaagccggt gtaggataca ggctaaacgc tctcccatgg cctccagggc cctgcatttt 42721ctcatcctgt ctacttcttc atctagttgt ttcccaccca gcgtaattgt aactgtttag 42781ttgaaagttg acgaggtctt ttgtcagctc tagaaatttc ttttcttttc tttctttttt 42841ttttttttaa gacagagtct tgctctgtgg cccaggctgg agtgcagtag cacaatctca 42901actcactgca acctccacct tccgggttca agcaattctc ctgcctcagt gctgaaatta 42961caggtgtgag ccacagcacc cagcccatca gctctggaaa tttcttcatc actctctctt 43021ccatgattgc cccatcccta ttttctccat gcctacctcc tggaatttcc atgagacaca 43081cgttgggcca tctcattcca ttttccgtgt ctcttagttg ctctgtcgtg ttttccatct 43141cttttatctc tatcctaaat tctggatagt ttcttcaagt atctttttag ttcattaatt 43201ctttttacag ctgtgtctta tttgtttact tacccactga gattttcatt ttattttatt 43261taattaatta atttatttat ttttgagaca gagtctcgct cttgttcccc aggctggagt 43321gcagtggcgc aatctcagct cactgcaacc tacgcctcct gtgttcaagc aattctcgtg 43381cctcagcctc ccgattggct ggggttacag gggtgcacca ctgtgcccag ctcatttttt 43441tgtattttta gtagagacgg ggtttcacca tgttggccag gctggtcttg acctcctgac 43501ctcaaatgat cctcctgcct cagcctccca aagtgccggg attacaggtg tgagctcccg 43561caccccgccg gccctttctt tcttttgagc tgagctatgt atttaatgaa accttttctt 43621gtgtatccag cRgttcagtg gtttcttggg gcatgtgatc tccttgagcc cagtcccaca 43681ggctgtaatc gaaggtcctc aggccacact ccccagcccc gctggctgcc tgcgcattcc 43741ttatcttggc catgttcctt ccacagggct cttgtccaca ctgtcccctc tgccagaatg 43801gtttgccctc ccccatgcca cctgctcctc ctcctgccac ctcagggaag gcctctccca 43861cctccctgac taggtcaggt cccctcgtgc cacagcctRg tgtgtctctc ctttaaatac 43921tcctcagctg cagttttacg ttctgttctg ggatgccctg gaaaatgctg ggctcctgca 43981gatatggccc gcagctcctg cgctctctgc tctatcccca cccctagccc agctgctaca 44041gcagcttccc tcggagggtt accatgcagt aggtcctgtt ctaagctctt tccacagatt 44101atctcattcc atcctcagga caaccctatg aggtaggatc tatgattatc cccattttac 44161agatgaggaa agtgaggccc agaaggctaa gtgaaggcat cctcagcaca tcctgagaga 44221ggagttcagg gtaggaatag cttcactagc acacagatga gaaactgagg ccYagagagg 44281acaagagtcc taatttgcac agcctggggc ctgggcacta ccccgagcta cttccttttc 44341cccagccctg gggcccggag ccagctttgt ccttcccatc tccgagggca tggactgctg 44401ctgccctgat gggcccctgt cctggccatg ggacccctgt cttccacagg ttgtcggcRg 44461cttccccagc caggcccaag tcaccgtcca ctgcctcaag atgcccaaga tctcctgcca 44521aaacaaggga gtcgtggtca attcttcagt gatggtgaaa ttcctctttc cacgcccaga 44581ccagcaacat tctgtagctt acacatttga agaggtgagg cgggtgcagg gagaggtggt 44641ggtgggggaa cctgactcac atatgggccg cagagggcag gggcctgggg gtctctgaag 44701cctccagatc cttctcacca cctctgctgg cactggttgt ctcttgcaca tggctcctta 44761caatcaaaat cacatcatgc aagtaacgag ggggtacaca cgtggtttcc acagcttagg 44821taatattttc tctcttttct tatttttatt ttttttagag acagggtctc actctgtcat 44881ccaggctgga gtgcagcctc acactcatag ctcactgcag cctcgaatcc tgggctcgag 44941tgatcctccc acctcagccc tcctgagtag ctgggaccac agacaaacac caccacacct 45001ggctaattta aaaaaaaaaa tttccttaga ggcggggtct tgttatgttg tccaggctgt 45061tctcaaactc ctggcctcaa gcaattctcc tgccttggcc tcctaagttg ctggggttat 45121aggcatgagc caccatgcca agcatatgtt ctttcattct tatttttatt tattatttat 45181ttatttactt atttattttt ttgacacaga gtctcactgt gttgcccagg ctggagtgcg 45241gtggcacaat ctcggctcac tacaacctct gcctcccagg ttccagcgat tctcatgcct 45301cagcctccca ggtagctggg attacaggca tgcgctacca cgcctgacta attttttgta 45361tttttagtag agacagggtt tcgctatgtg ggcccagctg gtctgagact cctggtttca 45421agtgatctgc ccgcctcagc ctcccaaagt gctgggatta caggcatgag ccaccacgcc 45481cagccaatat tctcctcctt aagctgagca gtggacaatg gtgtttatta cccgagtcca 45541caacccttta ttcattaggg ccacaagggt ttccaaattc agaacttctg tgaatttgta 45601aaagtagtat agtgtataga cactggatat attgcagaac accccaatag ggtgtggggt 45661ggcaccccac aatcaattct cctcaaatgc ctcaattggc atttctcctc atatgaagca 45721agataaatac catacatagc ttcctagaca atggccaggt tttgccaaca aaacttaaga 45781aaaaacttgt agttttcaga tcctgtttga ttttataatt acaaataagg aattaagaat 45841cagtattatc tttaatgctc cttagaatat cttcaatttt tttttttgag acagagtctc 45901actctgtcac ccaggctgga gtgcagtggt gcaatctcgg ctcactgcaa cctccacctt 45961cctggttcaa gcgattcgcg tgcctcagcc tccccagtag ctagctggca ctacaggcgc 46021gtgccaccac gcccagctaa tttttagtag aggcggggtt tcaccaggtt ggccaggcca 46081gtttcaaagt cctgacctcc agtgatccac ctgcctcagc ctccaaaagt gctgggatta 46141caggcatgag ccactgtgct cggccaatat ttcttaatac attaagaact aaagacaaga 46201gcgatacaat cacattgtgg aaaatataga aaaatagaaa gaagaaaacc actgtcacca 46261taaccccctt ctccaggggc cttcgatgtg gtgactgggt gcatttcctt ctggtgcttt 46321gttattttta aataaatttt ttatcttgga gtaactttat atttgaataa aagttgcaaa 46381atacagagtg ttcccatata tcccccaccc acttcccgct ttgtccacat cttacataac 46441catgctacag tgtcatagcg gagagattaa cattggtgca tgactgttaa ccaaactcta 46501aactttattt gaatctcccc agtttttcca ctgatgatac cctttttctt ttccaagatc 46561tgatccagga tagcacattg tatttagccg ttcttttttg ttgttgtttg tttgtttgtt 46621tgtctgtttg tttctgccca ggctggagtg cagcggtgat cctcccacct cagcttctcg 46681actagagatg tgcactacca cgtatttttt tgtagagaca gggttttgcc acattgccca 46741agctggtctc aaatccctgg gctcaagtga tcctcccacc ttggcctccc aaagtgttgg 46801gattataggc atgagccact gtgcccagtt tatttagcct tttaaatatc tctttaattt 46861ttgtattact ttccttttca tttttctgtt ttattccttt tggctttctc atgtggatgt 46921agacatatta ttttatttac ttattttttt gttgttaaga atctgaaaca ttgagcattt 46981gttaaaaaaa agaattattc sgccgggcac ggtggctcac gcctgtaatc ccagcacttt 47041gagaggctga ggtgggtgga tcacatggtc aggagttcaa gaccagcctg gccaatatga 47101tgaaacccca tctctactaa aaatacaaaa attagccgga catggtggca catgcctgta 47161gtcctagcta cttgggaggc tgaggtagga caatcgcttg aacctgggac gtggaggttg 47221cagtgagctg agatcgtgcc actgccctcc agcctgggca acagagtgag actgtctcaa 47281aaacaaaaaa agaaaagaaa agaaaaagaa agtgacttct caggtcctaa ccccaaagcc 47341acaggtgctg gggaactttc ctcggttttc agaagagcag tagctaagcc tggttcccgt 47401gtcatccttg cctctccagt ccctcagtgg aaagaatcag gggccctgag ctaggagggt 47461tgctctctgc ttcgggaaga gccctggctc acagcaaatt tggtttctct ccccaggata 47521tcgtgactac cgtccagScc tcctattcta agaaaaagct cttcttaagc ctcttggatt 47581tccagtatgt gctgcagaga agagaggggg cggtcaactc cgcaaacctc tccctggccc 47641cttggagtca ggcacagggc ggggtgttgg tggggaaatg tggccccttt cYtctggggc 47701atatgggctg actgcaggga gataagaccc tgcctagata gaatcttcgt ggggaagaag 47761gggctccagg aagaatggag ggctgccagg aagaagggcc tggcaggagg agagcgctgc 47821ccgagcaaag gcctggccgc cagaatagca aatctcaagg gaatagcaaa tctcaagaga 47881gtgccccaaa gggcctgagc tatgagacag aagcactggc tgctattctt agagtttctt 47941tcccagggga tgttacagga gggggcccaa tggagggtca aattatcatc gcttttttat 48001ttcaggatta caccaaagac tgtttccaac ttgactgagg taggtagtct tggatagact 48061gggggaaata agtcctgtgg gacctcctgc cttaaagaaa gcaggcggag ggccctaaag 48121gaaatcaggc aaccagacca aaagaatgtg gaccaggtgg tccatgctgt gtctcttgtg 48181acccttcttc tccctgccat gtcttttggg agagcccttg tgttgcaaaa atgagagtgt 48241ggtggtatgg attggggttt aggcagaaca gtactggcca agcagcgcct ccctggacct 48301caattttccc tctgtggaat gggctagcaa tcctgggcct ccccagggcg aaggaaagac 48361cactcaggaa gggcaccgtc tggggcagga aaacggagtg ggttggatgt atttttttca 48421cggatgggca tgaggatgaa tgcttgtcca ggccgtgcag catctgcctt gtgggtcact 48481tctgtgctcc agggaggact caccatgggc atttgattgg cagagcagct ccgagtccRt 48541ccagagcttc ctgcagtcaa tgatcaccgc tgtgggcatc cctgaggtca tgtctcgtaa 48601gtgtgggctg gaggggaaac tgggtgccga ggctgacaga gcttcccatt tcaccttgtg 48661ggcccttccc aggcagagct tcaggtgccc ctcttcccag tcattgatac ttagcggtcc 48721tggccccctt tcctctccct gctggtggYa ttgcacgcca atgactcggc cagatgccca 48781gacccctgtt cttggtttac ctgcagaata ttatctttgc caccccgcgg gatggctcaa 48841cccactttca ggatgcaggt ctcctaatag caacctgata tagcagaaag acccctgggc 48901tgggagtctg agacctagtt ctagcccagc cctgaacctc agtttccctt tctgtgaaac 48961aagaatgttg aacttgatga ttcccaattt tccttttgac cttgaaatgg tagaatattt 49021atccctttga ggtgactcgg atggtagact ctcagacacc atagcacacg tgtgctgggg 49081gtattttgga ccaggctctg ctgagagctt tctgctccct tccccacaga gtatgttgag 49141ctgactgtgc tttccttttg aacatctgct tgtccacatg gcttaggtag gagaggaagg 49201gcgtggaaac tggaatgatc ctagtggggt gtcttggcat ctcttggcct cattttcccc 49261atctgaacca tgaagctaaa actaggggat gtggattaaa tggttcctac aactacttgc 49321aaggagacca ctctgtgtgg ttgcaaagaa cactttgaga agctgtgtgg gaaagtttcc 49381ttcctagcag ggtagactca gctaactgca ggtcatgtgg ccattgtgga tgggttggga 49441gctcaagttt ggggcagaag ggaatttttt ttggcagcag agtggcaagc cctgccgcca 49501ggcaaactct gctcttcctc atcctcagaa gcacttgctc actctgctaa atcaaagtga 49561aacgcatgtt tacagaatat tggtccaaaa gggtctcagc atctcccact acccagggtg 49621gcagagcctc gggccggcct tgctccccaa gaagggctga ctggggctct gtcccctgcc 49681ccagggctcg aggtagtgtt tacagccctc atgaacagca aaggcgtgag cctcttcgac 49741atcatcaacc ctgagattat cactcgagat gtgagtacaa agcccccctc accagcccct 49801gttcctgggg agagaggccc agacaggatt cctggggtga ctgggggctg ttggggagac 49861agacagaggg gcctctacca gcttggctcc ctcctggtgg cctgggagtc agcccagctc 49921Rcccctctct cctactgccc ctcccttcag ggcttcctgc tgctgcagat ggactttggc 49981ttccctgagc acctgctggt ggatttcctc cagagcttga gctagaagtc tccaaggagg 50041tcgggatggg gcttgtagca gaaggcaagc accaggctca cagctggaac cctggtgtct 50101cctccagcRt ggtggaagtt gggttaggag tacggagatg gagattggct cccaactcct 50161ccctatccta aaggcccact ggcattaaag tgctgtatcc aagagctgSg gagtccttct 50221tctgtggctg gcgggtagag ggRgggggaa gggattgtct caccagtgcc gtccacctct 50281tttcagccct tccaaggcag ctgcccccaa accctccaag cttcatgatg actggaggaa 50341gaaatccaaa cttctctcct tgggactcac gatcctccct gatcaggtcc ctggatacct 50401tccaaattta tcccctttaa cccagcactc tccttgtctg accagcttcc tgcaggagct 50461gtcacacact ctctgtccct tggggttcca gtgctggaca gaaagtgagg gaggaccagg 50521gcttcagatc ctgaggatgg tgggagggga aggggccctc agaggtctgg tttctggtag 50581aaatggagga agacagagcc tttaaaaagg caaacgactt tattccagaa gataaactgc 50641tcacgagcct gagcttattt ccagctcacc tttttttttt tttttttttt tgagacggag 50701tctctctgtg acccaggctg gcatgcagtg gcacaatccc agctcactgc aacctccact 50761tcccaggttc aagcaattct cctgcctcag cctctcaagt atttaggatt acaggcaccc 50821gccaccacac ctagctagtt tttgtatttt tagtagagac ggggtttcac catgttggcc 50881aggctggtct cgaactcctg acctgcagtg atccacctgc ctcaaccccc caaagtgctc 50941atgttaaata tggtgaaccc caagtttttc ttcaaagaat cagtatgtct gtatgttcag 51001ctctcttatt ctttgattct ccattttaaa gtttaacttc ctggttctct tcgccccctt 51061gcttctaatt tcagtaaaca accttttcca tcagttttat tcagtagttc acacctgttc 51121ccctggtcac ttgctccatc ctgactcatc ccagtcacct gctttgacct gagtcacccc 51181tggtcacctg ctctgatgta agtcaccttt agttacccgt tcctaactgt ccttcctgcc 51241aaactactca cctcgccact ctggctcata cctctgctct ctttaaaata gccaatcgga 51301attagattag accgtgcggt ccaaccttag ccaacagggg aacaacatag cagcagggat 51361tacctgggtc aggaataaga accccttccc ctcccttgtt caactgtgct ctcaccattg 51421ttccatctat gaggagcacc ctttctacag aaactaaaaa ttgcttgctg agaaaattaa 51481atttatgttt gagtgctatt tctttgcagc agtggagaac aagcattcct aacactggga 51541ttgcaggcat aagccaccgt gcccagcttc cagctcacct tcttatgctc aaattgtgtc 51601ttctccttcc caccctcaag ctacaataca aaaagtttct tccaaccctc tcagtcttgg 51661aaggtagcag gctctatttt ctctgggtgt gtaaagaaaa tgcctctccc acattagcca 51721attgagacgg gaggaagcgc agcaagatgc tggctagtgc aaggtccctg gggtagaaag 51781aatcatgatg gattcaagaa cagtcaagag ggctggtgtg gccctgaaag caaagaacta 51841ggggcgagag atgaggccca accagcaggc agggtcacat aacaaaggtg ataaaagggc 51901ttggccttta tttcagcagc caggagagac agccaggagg ctggggctgt ggggagctca 51961gaagtaagat gccccttcca gtctgggaat atgtgagggg agggcaccat cttctgagcc 52021aggtttcttc ccagcccttc cctgcccttc caagtgcttt ctgaggcctg tttctcttcc 52081tcccctgggg cctaatgcaa gtgtctgact cactaagcag gagcatcatg ggcgggatgg 52141gcggctctta aagggccctg gcagaaggag tcagtgtgat ctcgccagtc cctgagtcca 52201acgtgggaat tctgggtctt gggatattgg cctttgaggt ggtgacacca gttaggagcc 52261acatcccacc caagaatgtc cccttgaaga ggctgggaag gcccaaggcc cccaccttga 52321tgctacaatg acagaatcag gataacttgg cagccctaag gcagctcttc cttgaatgga 52381aacttttatc tgcatgaggg gcagacagag aagtcacttg gagtcttaac ccttgtagac 52441cccagcctga ctgggactcc agaaggtccc agggctctgc tccagaaaga agggtagggg 52501catgccccca gcttccttca gtgaccttgg agaggtctcc ccagggctga gctgggaggc 52561acttccatgt cactgtctca tccaaggagt gcgattagca ggagatatgg agtccagagg 52621gtgagaggct taccaaaatg ggactgacct cYgagcccct ctcccaaatt ccagcccctc 52681catccaagcc tagaagctct gacctagtcc tattgaagtc ctcctggcct gggtgtgtgt 52741tggggggaat cctggctcca acccagttag gatcatttcc ataaagaagg ccacacctgc 52801tgcttcctct ggggaaaaac aagtgctccc caaggccagc accaggcaga agcacatcct 52861gccacctggg ctgctgacct tgggcaggat ggggcccggg tgtttggcaa ataggaaggg 52921tagagggaag gcctggcttt cagacctggg tctaatccca gctcccccca accccagtta 52981ctaattctgc catcctgggg cccctctgtg cctccatttc ctcttctatt caatgggatg 53041ataatacttt ccatgtagga ttctggcaag ggctgggtta gcccagagga ggagcccatg 53101gtggccaaga gcaggggctg cagagctggc ctgcctgggt ccacccttct gctcctctgc 53161ttctgtgtga tcttggccaa attatttagc ctctctgtgc ctcccttttc tcatctgtaa 53221aaagaaagat aacagttgcc atgaagagta aaggagaggc cgggcatggt ggctcagacc 53281tgtaattcca gcactttggg aggctgaggc aggggtatct caggagttca agaccagcct 53341ggccaacatg gtgacccccc ccatctctac taaaaataca aaaaaattag ctgaatatgg 53401tggtgtacac ctgtagtccc agctactctg gaggctgtgg caggagaact gcttgaacct 53461gggaagtgga ggttgcagtg agctgatatt gtgccactgc actgcagcct gggcgacaga 53521gtgagactgt cttaaaaaca aacaaacaaa aagattaaag gagagagcgg ctgcattttg 53581ggaggccaag gcgggctgat tgcctgagct caggagttgg agaccagcct gggcaacatg 53641gtgaaacccg gtctctacta aaatatgttt ttaaaaaaaa ttagctgggc atgcacctgt 53701agtcccagct actcgggagg ctgaggcagg agaattgctt gaaccggaga ggtggaggtt 53761gcagtgagct gagatcatgc caatgcactc cagcgtgggt gacagagcaa gactccatct 53821caaaaaaaag gagagagcac agtgtctggt atatcctaag tgctccacga gtgccagaca 53881ttggttatac ttcatggagg tgaggtacac agaggtgtct aggtcatgcg gactcactcc 53941atcctccagt taaccatgac tccctcccca cccacctcct gagttctctc tgctgtcaaa 54001ctttcctctc atatctccag tttcccaccc aaaagtccaa acactcaaga ttccttctca 54061tctccaaccc ataaaatctg atcggcttct ccacactcaa gtgtaaatga ctgatggcca 54121tttgattgac actggagggg ctggcacatt tttagcacat ttttagccaa aggtatgatg 54181tgctgatagc ggactctcag gccacaagcc agtctgagga cagggaaagt ttgaggaggt 54241cactcttcca gaaccctttg gtgataacct ttctggcagg cctggtaccc ccagagtgaa 54301aagggctcca gggtcaagac ccagctctgc ccctcattgc tgtgtgacct tcagcaactc 54361acttgattta tctggacctc agcttcttca tctgtctaat gggataataa tctttgtcct 54421gccctcctca cagggctgtt cagaattaaa tgcatctgaa aatgatctgc atttgtgtct 54481tggactgtgg ttatttgttc aagtgtctgt ctaccctgcc tgcacaggga gcgggtcagg 54541gtcttccctg ggacttcatg cacaggtctt tcctgggact tcatgtagga ccagccatcc 54601cgtcagtgct cagtgaacat gagctgcttc cctgtgggat gtctgggagg tgagtggaag 54661gccttcctag caggcacata ctggaacata atcaatccct cccatgcata cagctcacac 54721ccacattctt atccattccc accatgcccc tcagtggcct ggggagacta ggaatacccc 54781acttgacaga taaggaagct gaggccaggg gaggtcaagg catttgtctg aggttgcaca 54841accaggagtg gtgaagctgg gagtcacaca caaggaatca ctgcaaagtc tgtgtccttc 54901ccactctgag taggacttga accagggatg tccgatgctg atgcttgtgt cctgaccact 54961accttgcagc gtcctcaccc acacccaccc caggcctggg aggcagggga gcaccagcgg 55021tctgggagtg aaagttgctt ccctgtggga tgtctgggag gtgggtagaa ggccttccta 55081gcaggcacag ctgcccaccg gatgagtatg tccaggggga gaggagcccc ctgtcccagg 55141atgtgggcaa aaacctggag aagctgaagc tggactttga ggatggcccc tgacYccatc 55201ctgggacagc cacctcccct tccttctgcc ccctccagtc cctcctctcc ttccctgctc 55261tccctcctac ctctttccat ctcccccttt ctgcctgtga ttcctcctgt gactggggca 55321agatccttca cctctgtcct ccctgagcct cagtggctca cctggaacat agggtgatgc 55381ctgcagaagt attttagggt tactttatga gataaattgg tagactgttt ttcccgggtc 55441agccagccag gggttcggtg aataggagcg aacgctgctg ccattccctt ctcccctagt 55501tcatcctgga ggctctccgc tctccccctg gttctaagtc ccctcctcct gcaccgtatc 55561ccccctccca tccaacccag tccccactga ggcactgaga cagggtcttg gcccagggtc 55621cccacccacc tgagctccgc atgtgagccg accttcaccc gcctgcctca gtttcccgtc 55681cacgaaaggg gtgagcgccc ccacgtctcc tgggactgtc cttgggtccg atgcggggat 55741gtggggaggt gccagggtgt gggcgcagca gggagcgggt gtgttaggcc cccgcccatc 55801ccgcgcccga gccccatctg gctcgggctg gcacctcgaa tccacgtgat ttctcggcag 55861cagccgccag ttccatgcac tggcggagca gctctaggcg gcggcttcta ctttcagttt 55921cgtgcagagc gcggaggagc cgcgagcgct gagggtgagt gccgggagct ctgagggtga 55981gtgccgggcg tgccgcgggg ctgcgggacc cgggctgggg cgagcggaag ggagaggatc 56041ggggttcgaa ttctgcacgg agaggggtgg aggggatgtc agaggccctg gagcgggagg 56101tgcgggtggc cgggtggctg gccgatggat agctgggtaa gcggacaggg cgccagtggc 56161ccggcagcgc gcacagcatg cgccccggca gtttggagga agagttgccg gcccgcagag 56221gatggcagat gagggtcgcc tggatggggg gacacagtaa gcggctgtgc tcccctccct 56281gcgcagctcc tgtcacctac cggatccacc cactctctct gtcttttact ttgcaacttt 56341tcattgattt taaaacaatt acggaagtta cactgttcat tgtaYgcaat ttagactttt 56401tggcctaaac aactttttat tgtggaaaat tccaaacata tacaatagta gaaggaatag 56461aattaggttg agccttctgc acctgcgggt tttcatataa tgaagcccca cctatccacg 56521ttcagcttgg acagtcacga ccccaaggcc acctactcac gaccccaagg ccacctaggt 56581agcccaatct ccaccgctgg ttattttgaa acaaatggca gacagcatag catctcaact 56641gaggtatttc agaatgtact aaaagataag gcatctttta aaaacaaaac cacagtacca 56701ccagcatact acaaacattt taatagttat tccttaatgt caaatctcca gattatttct 56761aaggaacatt taacctcata agtgataggt gaatacgtgg gtgttgtttt gttacttgct 56821ttctgcattc ccatgtggtt ttcggtcact attctcaccc agaaagccag gacctgaaac 56881tggctggggt gatcattcga ggaggtcgga agaggctcct accttcagtg ccgtttgcgt 56941ccattttata agttggagtt cttatgtttc cttttttaaa aggttgaagt ttctgcagac 57001ctaaaaagca gtttgaaaac tggcagttcc cagcctctaa aacccggggg tagcaggagg 57061cagagagtac cccggggcca gatcctggag cctcattgtc ccttcaaaYc accttccgtt 57121tctctaaggg agaaataaac cttgaaaagc catgggcttt tcagatgtat ctagatgtat 57181ctgatgcttt tcaggtgtat ctagactccc atccagcgtg ctttctgcaa aacttactga 57241accatagccc agaatggcca tccatgatgc ccacccgcct tgtctcctga agcacattgc 57301caggtggacc tgcacatggt gtggacggca gacagactgg ggctctcaac aactggtatt 57361tgctggatga ccatgaacta accattcaat ttctcgaaac tttggtttct cgtctgtaaa 57421atgaacatat gcaaatctat cctacaaagt gtgttttgtg gattgaatga gatgatacac 57481gcccacccag tgccttgatc aaccttaaaa tgctgcacaa gtgtgagggg agatggatcc 57541ttgaagaaga cagacaagca gaggggaaag atcttggaag agtttcacaa tatgctgaac 57601tttattcctg gatcagaYac agagctgtcc gggtgtccac agggccgggt cagcctcctc 57661agaccccaac acacataccc tcttcccctc tgaggaaaat tcagcaaaac ttcttttgct 57721gttgttgttc tttattcctt tttttttttt tttttttttt gacgaagtct cactctgttg 57781ctcaggctgg agtgtagtgg cacgatcttg gctcactgca acctccatct cctgggttca 57841agcgattctc ctgcctcagc ctcccgagta gctgggatta taggcgcccg ccgctacacc 57901cagctaattt ttgtattttt agtagagatg gggtttcact atgttcgcca ggctggtgtc 57961aaactcccaa cctcagatga tccacccgcc tcggcctcct gtgggattat aggcgtgagc 58021catcttgccc ggactccttt tttttttttt tttttttttt aatagacatg aggtctcaaa 58081ctcctggcct caagtgatca gtctgcctct gcctcccaaa gtgttgagat tatgggcgtg 58141agccaccaca cccagccata acttcttagc atgacttttc ccatttgtta tgagttcaat 58201tgtgcctccc cacaaaagat atgctgaagg cctaaccctc ggcgcctcct aacgtgacct 58261tatttggaaa tagggctgtt gcagatataa ctggtgaaga tgaggtcata ctggagtagg 58321atgggccttg aatccaatag gaccggtgtc cttataagag aggagatgga cacatgaaga 58381cagagacata cggggatcag acaatcatgg acggaggagg aagagagtgc ggcgatgcat 58441ctaggagcca caggacacct agaatggcca gcagccggtg ggaaccagga gaggcaagga 58501cggatcctct cctccagcct tcagagggag cccggccctg accacacctc gatctcagac 58561tctgacctcc agaactgcga gagaagaaat ctcagttgtt ttaatgtacg cagcttgtgg 58621cactttgtga cagcagatac agcagtactg tgacaacact aggaaacgaa tacaccatcc 58681cagcatctct gctctgtttt ccatacagaa ttgaactaag gaccaggcaa gctaatgcaa 58741Rtccaaacac tgtaaaaact ttggaggcaa tctaaggatg tgagccagat ttgtttgcca 58801gatctttttt attttattta tttatttatt ttattttatt tttttttttt agtgagaggg 58861agtctcactc tgttgcctag gctggagtgc agtgatatga tctcggctca cagcaacctc 58921cacctcccag gtttaagtga ttctcctgcc tcagcttcct gagtagctgg gattataggc 58981acctgccccc atgcccagct agtttttgta tttttagtac agacaggatt tcaccatgtt 59041ggccaggctg gtcttgaact cctgacctca ggtgatccac ctgccttggc ctcccaaaat 59101gctaggatta caggcatgag ccaccaacac ccagcccata tattcatatt tttaatggac 59161ttatacatga aaatggtatc tcacttttaa aaataaatga tatggaatta tgcgttaaag 59221gRaaattaaa tcttattata tcatgtttta aaatttttaa tttttcatag ctaatacatt 59281tatgtagttt taaaaatgta aaacatttgg aaagatataa tgaaaaataa gtctccttcc 59341atctcctgtc tcccagccac ccaattgccc tccccgaaga caaccaatgt tatcaggttc 59401ttgtgtctcc ttccaaagac agtctaagtg tgtttttaca aacatggata tatacatata 59461tatatacacg tatatatgtg tatatatata tacacacaca cacacacaca catatacaca 59521tatatagaca cacacacaca tgtgtacttt tcccttctca caaatggcag tgtactatat 59581gtagtgttct ctaacttggg tttttttttt ttaaacttag tgcaatttgg aaatctttct 59641acatcaaaat tttaaaatct gccctttccc tctttctttt taacagctgc ctagtatttc 59701accgtatgac tgctctgtaa ttgatctaac agttgtagaa cacttaggtt atttcctgtc 59761ttttgctatt tcaacagtgc tgtaatgaat atccttggtc aggcatcatt tttcatacgt 59821gggagtgaat ctttaagaaa accaggagtg gatttgcaag gtcaagggga atatgcagtt 59881tgaacttgga taaataaggc aaattatccc cccttaaatg ttgtactaat ttttgctccc 59941accagctgga gggggagggg ccaaatttcc atatttgcaa atctgggaga cgaacaatgg 60001tgtgtttttt atgcctctta ttacgaatga gtttgaacat cttttcaaat atttaagagt 60061cacctgtagc tcattttcca taaactgtca gttcatatcc tttgcccact tttttattgg 60121cttttggtct ttttcctgtt gagttgtaaa agcacttttc atgttaaggg aatttgctct 60181ttgtctatta tatggttata ctgtcattta aaatggtggc atagttgctt atagaatgtc 60241tgaaccatat gcgtcattgt tagatattta tattgggtct cattttcttg tattatatat 60301gggatgtttt ccctgttttt tgtaaaaaaa aaaaaaaaag caagatatga cattttaatt 60361cttccaatag ttctcaccat aatggtgaag gaaaggaaat acgtttggga aaatacttcg 60421ataggatttg gggtggctgg aacctgtgtg caaaatggcc aacagggacc aggaccattt 60481tgggtgggaa gagagtgctt tgcatggtgt atgtgtatgg ggcggagggg ctgtatttgc 60541tcctgaaaga aaagggggtg gagggagggg agaagaaagg aaggagagaa ggtggaggga 60601ggttagccac agagggagat gactcccaga aacttcttag gggaaggatg gcaaaagggc 60661attttcggtg agtccaggaa tgccctctgc tccgtgtgag gcaggtacca gcactgcaga 60721caccggctct ggctcaccac ctgccatcat gagtgcattc agcttctcca Ytgagcttcc 60781acacaccagg ccctgctcta ggcactgggc tacagccggg aacacaacca actccctgcc 60841ttgtggagct gatgaggcag tagaaacaga caaacaagta aacaaagatg tgcatgtcat 60901atgaggtcag gtggaagtga ctgcagtgaa gaaggcaagg caggcgaggg tggagcatgc 60961tRgatagaga ggcagatgta gccttcttgt agggatgttg ggacatttga gagagaactt 61021gaagggagcg tggcagggaa ccatgggcca tggcatctgg aggaagaggg aaccagtcag 61081aggggatggc aagaaacaaa aaagtgggga gaagaagaaa gcagagaaga ggagaggagg 61141gagaagagga gaaaatgctg cttaaagtct gcttttgtgc tcccagatgc tcattgtgaa 61201tgacattttt gcaatttttg caattttgtt ttgtaattta ataatgggct gggctgggcg 61261cagtggctca cgcctgtaat tccagcactt tgggaggctg agatgggcag atcacttgag 61321gtctgcagtt tgagaccagc ctggccaaca tggagaagcc ccgtctctcc taaaaataca 61381aaaatagcca ggtgtggtgg cacgtgactg taatctcagc tactctggag gctgaggcag 61441gagaatcgct tgaacctgag aggtggaggt tgtagtgagt tgagatcgtg ccatgctctc 61501cagcctgagt tacagaggga gactcggtct caaaaaaagc aacaaactgc ttgaattaaa 61561gcaggagccc tcagctagag gagtgcccac ccacctcctc cccaggaatc gcagagctcc 61621caggagaggc catagcctcc tataggtcat ggcattgcag gtgggttggt gtcccagctg 61681gctgtttctg tctcaagaaa ggaagggctg ctcccgctga catccaggcg taagcaacgc 61741cctggtgaac atcctcgtag ctagagcttt gctcacattc tggatggctt ccttaggctc 61801aatcctcaaa gtggactcct aaagggatat atgaactttt attttttaaa tgtttgaaac 61861cgtgttgtca aatttctctc ctaaaagatt gtgccaatgt agagaggctg ccgaccgctg 61921gaagcttccc aatcccccag cacctatccc ttgtgggtgg gatggatcct gtaccatcgc 61981cagaccctca agaacccctt gtcctgcaYg gtcccttctg tccctcctgg tgaaatggaa 62041ggagcatgca tgccttggga agctggtgct ttgaggcatt cagatcttac cgtggcatca 62101tgtttgaaga gagaaatagc ttgctgtttt gtgtgtctct caatagtctt attcactgag 62161ttcttgaagg tgtcctgtgt gtttctggac tgcagaatcc ttttattgaa atggacaggt 62221cagtgttttc ccagagaaag acctatccag taggagttca caagaggatt ttaggaggta 62281tgtggagagg gcatccgatt ccattgaatc gtaaagtgac aaatttgttc ccttttcagt 62341tctttttcat tcttccagac tagtgaaaga gaaagcctca gtcaggtgct aacgcatctg 62401taaacctctc acactcttga tattttcagc aaggggaggc caggcctccg gtcagagccc 62461tgggtctgca gcaataccca gctacagttc aacagcatca tagtgttttt tgttttcctt 62521ggctttcttt ctttcttttt ttttctagac aaggtctcac tctgtcaccc aggctggagt 62581gcactggttg cacagctccg tgcatagttt cctgcagcct ctatttccca ggctcaatcg 62641atcctcctgc ctcagcctcc caagtagatg ggactacagg tgcgcaccac cacacctggc 62701taattgtttt ttgtttttat ttttgataga gacgaggtct cattacgttg cccaggctga 62761tctcaaactc ctgagctcaa gcagttttcc tgccttagcc tcccaaagtg ctgggattac 62821aggtgtgagc ctccatgccc cacccttgag tttcttttcg atgatacctt cagttcgtgg 62881caagtggtac ttcaaaaaat aaagttaaaa gtgacatggt ttctagaaaa gctttaagga 62941aatggtgttc tggctttcag ataagtcaaa aaaaatctca aacatgagta aagagttttg 63001gaaattatac acttacccct agagggtgag tttgtggggg aaatttcata atgtatactt 63061ccagaatatt tagaactttc caaatgagta caggttatgt tcgtaaacag gaaaaataat 63121aaagacataa taaaatgata atttagtttt aaaagacgac catttgttga aagcagtatt 63181atatttcagt taggaaatgg cctctggaat cagcatagat tttattgttt tatttatttt 63241gtttttgaga cagtgtctca ctctgttgcc cacactggag tgcagtagtg tgatcacagc 63301ccactgcagc ctcaacttcc tgggctcagg tgatccttct gcctcagcct cccaaatagc 63361tgggactata ggtgcacacc actatgccag gctaattttt ttgcattttt tgtaaagaca 63421gattttcacc atgttgccct ggctggtctc gaacttccag gttcaagcaa tctgcctgtc 63481tcggcctccc aaagtgttgg aattacaggt gtgagccact gtgcccggcc agcatagatt 63541ttaaatccac cttttgttgc ttgacttctt ggagcctgtt tcttcatcta tgaaatgcag 63601gaaacgtgcc tggaaatatt gcttgagatt ctaatgggga ggtgcaggtg gaatactgta 63661gcctgatgcc cagcctatgc cgggaactca gtacataaca gccaatgagc attggctgcg 63721gactggacat gtcccctcct tgtgctgtag tattaatgtc tccaaccctc acagtgatcc 63781tgtgaagtaa atgtaaaatc accacccaga tgaggaaaac agtggcacag agaggttaag 63841aaaactgtcc aaggttgcac agcactaaaa tgttgaaatc aggatttaaa cccagccctc 63901ctgaccccag aggatctttt ttcaaaaatt atgttctttt attttatttc gagatggggg 63961tctttcacag tcacccaggc tggagtgcag tcacaatatc acggcttact gcgcctcaac 64021ctcccaggct caagtgatcc tcccacctct gtcccccaac ccccagttgc ttaggagcac 64081aggcacacga caccatgcct ggctaatttt tgtacttttt gtagagatgg ggtttctcca 64141tgttgcccag gctggtctca aactactggg ctcaagcgat cctcctgctt cagcctccca 64201aagtactggg atcacaggtg tgagccacta tgcccagcat tagaggctct tttaacagct 64261ccacaattcc ctgtgagatg ggctgcatac aaagcattac tcctgcttga catatacaca 64321aaccatggcc caaagggagc tgggacttgt ccggatgtca gcctctaagt cagttttggg 64381tcagtgagac ccaaaaccaa ggctctagcc gtctagcccc tccaaccttt cttctgcccc 64441agttttatgg gtttggcaat gtctgccagg atgggaggga gcagttcaga tgatccagtc 64501tggaggcaaa attctgctaa attcaaaggg cccagcctca agtcactgat gtcttttttt 64561tttttttttt actttaaaaa aatgtattMa ttctaaacca cacaagtaag acacgaatca 64621gtcttccttg cagaaagtta aactttttat acataaagct gatatctatt ttactcactg 64681cttccagccc catgcctytc ccctctcctg cagtaacccc tatttgatat gtttccagaa 64741ctttatctaa accttcacat aataaaaata attcttatat agggcttact aacttccaag 64801cactgcttta agagctttac atatttaagc cccacaataa ctctgtgagg taggtgctgc 64861tcttatcccc attttacaga tgagaacatt gaggcccaga gaggctatgt aactggcctg 64921gcgtcaccca gcctgcagtt ggtggaaagg tcagtcccaa atctaggcag ccttgctccg 64981aaattcatgc tcttagcctc taagttataa aacatgctat tatcgacctg tgggaagaaa 65041cagtattgct gtttgttgat gtgtattttg agacaagtga tatcatgttg tgcatatcat 65101tatacaatgt gtcttgttta ttccatgata catcttgaag atacagccac gtcaacaaaa 65161aataagtccc tttcctgtac ataatcccac agcgtggtta caccaaactt tattatggct 65221gtctagggtg tttaaagacc gctgctggaa tttctttatc caagactctg tgtgtcttag 65281cccattgggg ctgctgtaac aaagtgccag agactggctg gattctaaac aatagaaatt 65341tatttctcac agttctggag actaaagtct gagatcagag tgccagcatg gtcgggttct 65401ggcaagagcc cttttccggg ttgccgatgg cggacttttc attgtatcct cacatggtgc 65461aaagcaggct agagcattct cttgggtctc ttttataagg gcatcaatcc cattcactac 65521ggctcctccc tcctgaccta attacctccc ccagacccca gctcctaata ccctcctatt 65581aggggttaga gtttcaacat gtgaatttca ggggagcaca aatattcagt cgatcacatt 65641gtgtattctg ggccccaagg ctgtccattt tgctttctgc tgagaagttt agtggtaagg 65701tcaaagggta gaagcgcagt aagatctaga agtcactgct gatttgccct acaagatggg 65761caaaccactt cccagcctgc agctctgagc cctggatcca ggccttctct cctttatgtc 65821tcgagcttac tctgtcccct gccttactta ccaaaccaaa gcaagactcc tctgggacat 65881tcctgccagg ccccatggca aggttggagc tgaggagggg acttccaaac cttggtttta 65941ttttcacttt ggcttgggtg tgccaagccc tggcagagcc acagagttgg ggcacgaggt 66001gccaagaaac agctcccagt ctgaggaagg tgctctgccc tgggaagggg ataRagctga 66061gtgttggtta tggggagaca tcctcaaggt ctccatctaa atatggtcaa cttggtggcc 66121ttcctgtccc cagggccttt ggRgctctca gaggacaaga atcactcagc tctgatttgc 66181tggagaagca gagccccagg tgggaggtag cgaggagcct gtctctcccc actgggagtg 66241gagagctggg tgccacagcc tcaaacagtt gttcccagac atcggcacat ggggaacccc 66301ccgggagctt ccaaaagtgc cagtgcctgc gttctacctc ctgggatggt gctgtgatgg 66361gtctggagtg tggcccattg ggatttttta aagaccctct tcccaggtga tgccgtcgtg 66421cagacaagca tgggaaccac tggcctggaa gccccaggga gaggcctctg gggcccggga 66481cacgcaagcg ggagccttct cctagcagcc ttgctagcca cccacccctt cctgtgggtg 66541ctagacccaa actgccaggg ctgagcagga gaagcaaggc caccagctgg ccacctgctg 66601tcccagccag gctcaggctg gcagtagggc accccagtcc tggaggaggg tgcatctgga 66661aagcttgggg gctcttggtg gaaagttcaa gcctttagca taggaaggtg gctggggtga 66721aggaggcagt ttaaagacta gtgatgtggg ctgggaccca gattgctggg tttccaatga 66781ccagaagaag atggccttgt tggactgggg acagggacca cccaggctca ggcctgaggc 66841tgggagtgca ggcatgaatt tgagtgcagg ctcaggaaca tccccagggc cgccaggcag 66901ggagacacct cttcaactcc tggctcctcg actctagctg gtgacctagg caggtccctt 66961tcccactgtg agcctcaatt tcctcatgtg taaagcatca tgactgtgtg ttttaagtcc 67021tttagggccc tatctttgtg agggacaaca gagacgatgg ttttcacagg caacagtcac 67081ccctgcatca tcactgactc acagaaatgg atatgcccta taaggacatc tcttgcttgc 67141cctgccagca tttgtgggcg ggggaggaaa tggccactaa ccattcctga aggcacctcc 67201caagggaccc ctgaaaggag ggtgacgcct cccagaaaga gaccagtggc ccagcccagc 67261cctgctctta gttttgtttc ctctgctttg aatgccagca aggcctttcc ctcccagggc 67321ctcagtttcc ccatctttac aatgggcaca gcatagaccc catcactggg gctctttctg 67381ctctgccacc ctgtgcatcc aggctcaggt gttatccgaa gccctgacat catcagccat 67441tcccgggggg aaggcagagg cgctgggttc tctgtgtccc cctggtaggg cttggtgtag 67501gattcaggga ggattggagg gcctctgtgg cctcaggtcc tcccccaggg tccccaggcc 67561ctgacctgtc cacctggacc taaagaacca acaacagcag cactttctgt ttctttgagg 67621tatttctgtc caaatgtctg tagtcatgac tggggtgcct ggctatcacc ccacaaactg 67681catcctttct gatctgcaca gatgagaaaa ctgaggtctt gtttcccctc catcccaaga 67741cctaatggga cctcctcctt aatctaatcc tcaagaggca gaaacaggat ggaaagcaaa 67801ggaatggcag ccagaagtct gYggctccac tcctgtcctc tctgcatccc tcacttcctt 67861ccttacacca ggcgtcaggg gctggagaga cggaagaagc gatagcaacg acaaccacac 67921tagtggtaat catagcttgt gtggattgaa cctttttttt atttttcatt tttaattttt 67981gtgggtacat agtaggtgta tatatttatg ggttacatga gatgttttga tataggcgtc 68041cagtgtgtaa tcacatcagg gtaaatgggg tttccatcac ctcaagcatt tatcctttgt 68101gttgcaaaca attcaattat actttttgtt atttttaaat gtacagttaa attattactg 68161actatagtta ccctcttgtg ctattgaata ctaggtttta ttcattctaa ctattttttg 68221tacccactaa ccatccccac ctcccccacc ctggcctcct actatacttc ccatcctctg 68281gtaaccatcc ttctactctc tatctccatg acttaaattg ttttgatttt tagatcccac 68341aaataagtga gaacaagtga tgtgtgtctt tctgtacctg gcttatttca cttaacataa 68401tggcctccag tgccatccat gtccttgcaa atgatgggat ctcattcttt ttgatggctg 68461aatagtactc cattttgtat gtgtgccaca ttttctttta ctcttttatt cttttctttc 68521tttcgagatg gactcttgct ctgtcaccca ggctggagtg cagtggcacg atcttggctc 68581actgcaacct ctgcttccca ggttcaagca attctcctgc ctcagcctcc caagtagctg 68641ggattacagg catgccccac catgccctgt taatttttgt acttttagta gaggaggggt 68701tttgccatgt tagccaggtt ggtctccaat gcctgacctc aggtgatcca cctgcctcag 68761cctcccacag tgctggaatt acaggcgtga gctgccgtgc ccagstgcca cattttctct 68821gttcattcat ctgtcgatga acacttagat taattccaaa tcttggctat tgtaaacagt 68881gctgtcataa acatagtagt gcagatatct ctttgagaca ctgattcctt tcttttgggt 68941atatacctag cagtgggatt gctggatcat atggtagctc taaatttagt ttagtttttt 69001tttgtttttt tttttttttt gagacagagt ctcactctgt ctcaaggctg gagtgcagtg 69061cgtgatctca gttcactgca acctctgcct cctgggttca agcgattctc ctgcctcagc 69121ctcccaagta gctgggacta caggcatggg gcgccactac gcccagctaa tttttgtatt 69181tttagtagag acagggtttc accatgttgg cgaggatggt ctcaatctct tgacctcatg 69241atctgcccac ctcggcctcc caaagtgctg ggattatagg tgtgagcctc cgtgcccagc 69301cctattttta gttttttgag gtacctccga actgtcctgt agtggctgta ctaatttaca 69361ttcccaccag tgtatgaggt ctcccttttc tccacataga ttgaggtgtt tgtttgtttg 69421tttgtttgtt tgtttggaga tgaagtctcg ctctgtcgcc cagactggag tgcagtggcg 69481tgatctcagc tcactgccgc ctccgtctct tgggttcaag ctattctccc acctcagcct 69541gggattacag gcgcctgcca ccacgcctgg ctaatttttt ttattttaag tagagacgag 69601gtttcctcat gttgcccagg ctgctctcaa actccggacc tcaggtgatc tgcctgcctc 69661agccttccaa agtgctggga ttacaggctt gagccaccat gcccagcctg gattgagttt 69721gtatcatgtg ccaggttcca tgtcagggat tccacatgcc ccatcccaca accatcttgc 69781caggtgggca ttatgatgac cccccatagc agacactgtc agagctcccc caaccccatc 69841tctgcccgga tgcccccact cactgtgggt gacacctgca tccttcttcc ggggtcgcct 69901ttgcctggtg ggcattgcct tgctcagaga tgcctgggca gtcatgcctc cctcccccgc 69961ccaatggggg tgcagtgacg tgaagtgtga ccgaccaccc agaccccttg cacatgcgca 70021ggatggacct cttctatcat cacgctccag ttctcccatg tggcaggctg aagcRaggct 70081tttgaaacca tatctttgct cagctgcctc tgctacccta tctttcttcc accaccccct 70141caataaacaa cttccacagg aacccccttg ggctctgctt ctagggacct gacctagaag 70201atcctcattt tacagatgag gacgctgagg tacgaagagg ctgcgtaacc taccagggtc 70261acacagcttt ggtgtggagt agggactcaa atccagggcc ctggtacgga ggcatttgcc 70321ctcaccaaga ttcaggcact gagagaagcY gacgggccta gagaatggca agacttgaga 70381tagcagaagg aaggagagag ggtgagaaat ggatccccgt gctgaggtct ggtctggaag 70441gagaagtctg caaggtagga aaaagaggaa aggggacgtg aggtagggaa gcaggatgtg 70501caaggcgggg acttgtgaaa gagaatgccg gggggtggtg agcagcatct catgggcagg 70561tcagcaggtg caggagaggc ctaggagggc cggggactgg atgatgaaag ggcatttgtt 70621tatttatcct ttgtttttgt tttttgttgt ttaaattgag accagctctc acaatgttgc 70681ccaggctcgt ctcgccaact gtctgcccac tcctggcctc aaacgatctt cctgcctcag 70741cctccaaaag tgttgggatt acaggcataa gccaatgttt attcgtcctg agccttccct 70801ggacacctga tatgtagctg ctttgtggtg gtcacagttc catggtgtga ctttatcctg 70861gaggcagtgg gatgctgata gcaagttcag acaggcatga ggccagacca gagctgagtt 70921ttcaagataa ctccagccag aggtatgcgt gcatgtgcac atgtgggtac atgtatgtgt 70981gtgcacgtgc atgtgtgtat gagtgtggac tagagatgga atggctttct gagcaaaggg 71041aacagccaag gcaaaggcct gtgggctgca gggagagaac aaggtttgat ctctgcactc 71101cctcctctaa gctctgtgtc aacagcattt tcaggacagc aggaaggatt cccaaagagc 71161acagcagcga gggcagttgg agggaggcgt ctgctgggac actgaaacca gggYggggta 71221aactcccctc gcacctcctc agactcagag cccccataaa tcttccaggt gcaccccagc 71281ccttttccct ctctctgtct gaactggggt ggcatgatcc caggactgca actccacgtg 71341tctggattcc ccttactgac tgcgtgaccc tggccatttt ccctccacct ttctctcagc 71401gcccccattt gattactcgg cctcaggggt gcactgggga ttcttagagg taacacttcg 71461aacggaaagc cagaggtcct ggtgccaaaa tgcaaatgtg agggcttgaa agttcatcgg 71521gttttatttt ctctgttccg tttgcagtag ctcttctttc ttgttttcta tagaacagta 71581aaaaacttta tgcatttgaa tatcgtgaac cttgtaattc atatgcaata taaaaactac 71641tagctatgca tggagaaatc aattgaagaa cagtggctta gcatgtcttt ctcattcttt 71701gatggctacc tcaaatcaaa tcaatatgaa cttggggtca ggcatggtgg ctcacacctg 71761taatcccaac acttggggag gccaaggcag gaggatcact tgagcgcagg aatttgagac 71821tagcttgggc aacatagcgg gacctcatct taatattaaa aaaaaaaaaa agagaaagaa 71881aaaaagtgaa cttgggaggt ttgaatgaca actcgtatag ctgaagtaat agatatattc 71941aacttttaaa acttaatttt cattagataa cacatgcata tggtataaca ttctaggcca 72001ggtgcagtgg ctcacatctg taatcccagc actttgggag gccaaggtgg gcagattgct 72061tgagcccagg agttgaagtc tagcttgggc aagatggcaa aaccccatct ctgcaaaaaa 72121cacaaaaaat tagctaggtg tggtggtgtg cacttgtagt cccagctact caggaagctg 72181aggtcgggga atcatataag cccaggaggt ggagactgca gtgagccacg accacaccac 72241tgcactccag cctgggtgac agagcaatac cctgtctcaa aaaaaaaagg aaaaaaaatt 72301cctttcactg gggacaatta cctttatgaa tttcttccag aaatattaat gtatttgtat 72361acacattttt ttttgagaca gggtctcact ctgtcaccca ggctggagtg cagggatgca 72421atcttggctc actgcagcct ggataccccc aggctcaagc aatcctccca cctcaacctt 72481gcgggtagct tggacaacat gtgtgcacca ccactcctgg ctaatttttg tattttttgt 72541caagatgggg ttttgccatg ttacccaggc tcgtctcaaa ctcctgacct caagtgatct 72601gcccgccttt gcccctcaaa gtgctgggat tacaggcatg agccaccata tttatttttt 72661ctttacacaa aggaagcata ttctacaccc tgttttgccc cggctctctt ttaaaaaaca 72721gtgtatcttg gataccattc catatcattc cataaagttc atcttcatcc tctttttttt 72781tttttttttt tttttgagac agggtttcac tctgtcgccc aggctggggt gcagtggcac 72841tatctcagct tacctcaacc tcccgggctc aagtgatcct cccacctcag cctcctgagt 72901ggctggatgc tgcttctttc tttttgatgg ctgcaaagaa ttccattgta tagatgtgcc 72961ataatttaat cataatatgt cctctttgat catttaggtt tttccaatct tttgctatta 73021caagcaaggc cacgttaaat atccctgggc atattatttt gcacattatg aagatatctg 73081taggctaaat acctgtgtat ggaattgctc agtgtgatta catggcattt accactttgg 73141tagataaagg ttgtatcctg ttacatgtcc acggatgaat ttcttccaga aatattcatg 73201tatttgtata cacatttttt gagacagggt cacaaaaccg ttaacacatt attattatta 73261ttattattat tattatttca cattatttag ggaagtttca tttttttgtg agctatcact 73321gataatttct tatttactca tcatcttgta tttctcttcc ttgcacattg gggaaatgtc 73381tattctagtt tttaatgtaa atgttcttgt ggctttttta gaaaaaatca aaatgtgcac 73441attaaaaagt ttgaatggtt caaaaggtcc taaaaagggt ccacagtgga aaaggctctc 73501ctttcccagc aggcccagat ccctcctcag acacaagcct gtcgtcagtt tcctgtgtag 73561cttcccagag ttggtcacta tgtatgcaaa tacacacaca tttaaaatat acacactcca 73621cgtccacgtg gaatgatgca aagcatctaa gtgcccgtta attcacaacc ttgccaactc 73681agtaatatcc ttgcgaatct aatagctaga aaatggtgta tctttttagt tctaatgtat 73741tatatttatt ctattttctg tgagcttgaa catcttttca tatatttaaa agtcattggg 73801gccaggggtg gtggctcatg cctgtaaccc cagcactttg ggaggccgag gcaggtagat 73861cacctgaggt cagaagttcg agaccagcct ggtcaacatg gtgaaactca gtctctatta 73921aaaatacaaa aaaaaaaaat taatcaggca tggtggctcg tgcctgtaat cccagctatt 73981gggaggctga ggcagaagaa ttgcttgaac tggggaggtg gaagttgcag tgagtggaga 74041tcgtgccact gtactccagc ctgggcaaca gagtgagact ctgtctcaaa aaaaaatttt 74101taaatgtcat tggtgtttcc cttttttgta aaccttttat gttctttgcc tagttttcta 74161tgaggtcatt ggctttttca gtgttggttt ttagtaactc tcgatttatt aaaacattta 74221tacatttata gctttgttta tgatgtatac atttataacc ttgtctgtga tgcagatatt 74281tttccaattt gtcatctggc ttttgacctt atctgtggta ttctttgaca atacaaaaat 74341tttaatttat atctgatcag tttcatccag ttgttttttt cttttttcat gatttctgag 74401ttttgtgtca tgcttagcaa gatgctctct actctgaaat tattttctgt aaaatatttt 74461tttcagccag gcatagtggc tcacacctgc aatcccagaa ctttgggagg tagaggtgga 74521aggattattt gagtccagga gttcaaggcc agcctgggca acagagcaag accccgcctc 74581tattttttaa aagagaaatt tttttaaaat actttttccc atgttttgtt tgagcactta 74641cattttctta acttttttgt cgtgaaacat aacacataga aaaatgtaat gtaagtgtac 74701aacttcatga attgttataa attgaacata tagctttctg gtactttcat ggtttaactt 74761cttacattta aattttggtt tcatctagac tttattttga tataattgtg aaatagaaat 74821ccatttttat tctaaaagaa cactttttaa atgttcatga aatgttttaa aatttattat 74881ataccaagca agaaaaaatc tcaacatagt cctcaaagca gaaattgcac aggacttatt 74941ctttgtacag tgtaatgcac aaaaactaat aagaaagcct taaaaacaac cctttattaa 75001attactccat tccaaattat aatttcaatc tacttagcac ataagtattg atatacattt 75061atcgaaattg gccaaaagtg ttttattatt ttttaaaaga aaaagcctag caatggctta 75121agcaataaag tttttaattt taaagaataa caaacagcct cactaaatca gaggaaagaa 75181ataaaaagat aaaagcaaaa attacagaac tagaaaaggg accattccag caaaattgat 75241aaatgaaaac aaaaatttgt ttttcaaaca taagagttac caacggctgg tgaatctaaa 75301aagaagaaaa aacaaagtaa actaaattaa atgtgaagcc atgaagagat ttaaaattat 75361aagaaaatag gccgtgcacg gtggctcacg cctgtaatcc cagcactttg ggaggccgag 75421gtgggtggat catgaggtca ggagcttgag acccgcctgg ccaacatggt gaaaccccgt 75481ctctaccaaa aatacaaaaa ttagctggct gtggtagcat gagcctgtag tcccagctac 75541ttaggaggct gaggcgggag aatcacttga acccaagagg tggaggttgc agtgagccga 75601gaccttgcca ttgctctcca gcctgggtgg cagagtgaga ttccgtctca aaaaataaat 75661aaataaataa agctgggcgc agtggctcat gcctctaatc acagcacttt gggaggccga 75721ggtgggcgga tcacaaggtt aggagatcga gaccatcctg gctgacatgg tgaaaccccg 75781tctctactaa aaatacaaaa aatgagctgg gcatggtggc gtgtgcctgt ggtcccagct 75841actcgggagg ctgaggaaag agaatggcgt gaacctggga ggtggaggtt gcagtgagct 75901gagatcacgc cactgcactc cagcctgggc gacagagtga gactccattt caaaaaaaaa 75961aatacaagaa catattatat acaactgtga gctacaccat gtttgaacat cttgacaaaa 76021tgagcaactt tctcaaaact aaattgccaa atgttcattt ttaaaaagcc aaaaataacc 76081aaccaacaca atgaggataa atttaaagtg atcaagtttg atttttttaa aaaactgttc 76141aaacatcttg gtagtgctgg gcaggaagtt ggctttcatt ctgggtgaga tgagaaggct 76201ctaaggggca taggacctga tgtcatggga cctgatgcag gctctgtctt aatagctgac 76261tcctgcgtgg agtaggttat tgggggatgg gtggacagag accagggagg aggctggtgc 76321aggaatccca gtgagaaatg atggtgtcca ggctaggcca tagcagtaga ggaagcaaga 76381ttctggaatg cacttggaaa atgaagccat ctttgggctt gagctcaagg aagcatgaag 76441ctgcccctaa ctgaagtggg gaaggtggtc tggggagcag tttaggagga gagatcagga 76501gctcagtttc ggacttggaa attttgaaac gcacatgagg catccaggtg gagttgggag 76561aattaaataa tgctgaaagg tactcacttg gcaagtagca aatattcgct atattgtctg 76621ttcaactttg tcaactgctt ttccagtaga aatgtagtgt ccttatcaca catgcataaa 76681tgttaaactc aaaagtaaaa tgaaatattt tctcagtaga tatgaataac tttttaattt 76741ttttgttttt tgacatgcgg tctcactctg ttgcccaggc tggagtgggg tggcgagatc 76801atagctcact gcagcctcaa actcctgggc tcaagcaatc ctctgccaca gcctcccgat 76861tagctgggac tacaggcaca caccaccacg cccagctaat cttttatttt ttgtagagac 76921tgagttgcac aatgtagccc aagctggtct ccaactcctg agctcaagct atcctcccac 76981ctcggcctcc caaagtgttg ggattacagg cgtgagccac tgcacctggc tgctttttaa 77041ttgtaatatt catcctccat ccatcatact tagtgcaaat atttttcccc acctattgcc 77101ttcctatttc tcttttattt cacgtcattt agggaagttt catttttttg tgagctatca 77161ctgataattt cttatttact catcatcttg tatttctctt ccttacacat tggggaaatg 77221tctattctag tttttaatgt aaatgttctt gtggcttttt ttaaaaaaaa atcaaaatgt 77281gcacattaaa aagtttgaat ggttcaaagg gtcctaaaaa gggtccacag tgggaaaggc 77341tctcctgtcc cagcaggccc agacccctcc tcagacacaa gcctgtcatc agtttcctgt 77401gtagcttccc agagttggtc tctatgtatg caaatacaca cacatttaaa atatacacat 77461gccacagctt tttaaaattt gaatttatgg gccatgtgca gtggctcacg cctgtaaccc 77521caacactttg ggaggccgag gcaggtggat cacctgaggt caggagttcg agaccaacct 77581gaccaatatg gtgaaacccc gtctctacta aaaatacaaa aattagctgg gcatggtggt 77641gggtgcctat aatcccagct tctcaggagg ttgagacagg agaatcgctt ggacccagga 77701ggcagaggtt gcagtgagct gagatcgtgc cactacactc aagtctaggc gacagaggga 77761gactccgtct caaaaaaaaa aaaaaaaatt gaatttagac tcaggtgcag aggctcacgc 77821ctgtaatccc agcacttacg gaggctgatg aggggagaat cacttgagtc cagtagttca 77881agaccagcct gggcaacaca gggaaactat gtctctacaa aaaaattaaa attagcctgg 77941catggtggag tgtgcctgtt gtctcaccta ctcaggaggg tgaggcaggg gaatcacttg 78001aactcaggag taggaggctg catgagctat gattgcacca ctgcactcca gcctgggtga 78061cagagcaaga ccctgtgtca aaaaaaaaaa aaaagaaaag aaaattagac taccatcata 78121gatggtacaa acacttccca gtttgttgct ttcgttttgt ttgttgccat ttttactata 78181cagaaaaaaa aggcaataaa gaatacatta ttataatatg ttaatttcta ctgcattatt 78241tttaacaact tcattgaggt gtaatttaca taccgtaaaa tctatccatt ttaggtgtag 78301gattcagtga tttctggtaa atttactgag ttatgtgacc atcaccacaa tctggttttg 78361ggacattttc attactgcca taatattcct caaacccatt tagaatcatt cctatttctg 78421ctcccaggcc caggcaacca gtaatctact tgctgtttca atagatttgc cttttctgga 78481tgttttacat aaatagaatc atacaatacg tgatctttag tgatcttttt tcacatggca 78541taaacttggt tcctttttat gttgaataat attccattgt atagatatgt cacagtttgt 78601ttctccattc atgagctttt gggtatttgg gttgatttca ctttttgact gttatggata 78661atgctggtac aaacattcat atacaagttt ttgtgtagat gtatattttc atttctcttg 78721gaatagacct aggcgtggaa ttgctggatt agatggtaat tctattgttt tacattctaa 78781ggcaggggtc cccaaccccc aggctacaga tcagtgtcgg ttcatggcct gttaggaact 78841gggccgcaca gcaggtggtg agcggccatc gggcgagtat tactgcctga gctctgcctc 78901ctgtcggatc agcggtgcca ttagattctc ataggagcac aaacccagtt gtgaaatgcg 78961catgtgaggg atctaggttg cgagtttctg atgagaatct aatgataaat gtaatgagct 79021tgaatcatcc cgaatccatc ccccaacccc aacccccgtc cggggaaaaa atgatctctc 79081atgaaatcag tccctaggcc aaaaagtctg gggactgctg ttctaaggaa ctgccaaaac 79141tgccaaactg tttttcagag tggctggacc attttacatt cccaccggca atatatacgg 79201tgtcctgttt ctttacatcc tcatcaacat gtgtcttttt cattacagcc attctatgtg 79261ggtgtgaaat gctatgtggc tgtgtttttc atttccatgc agaaattttt aatttaattg 79321cttagttaaa aataattctt taaatttaaa acactcatat tgccatatgt atatttttat 79381actacaaagt gatacaattt ttgttaattt tcttgccttg attactcttt acctgtatag 79441tatttaaatc tttacataag atcacaatta tagtgaatag taatttattc aactcaaaat 79501cttcatagga tttagaaaat ttaagtcctt taccaaaggg attgacttYt aacttcatta 79561aaggttgcga acagtatcaa atccttggta aagccacatt caatctttgg cactccagga 79621ttttgagcta taactgtagt gatcactaat atgtcatttc taagctgacg gtcataatga 79681ctaaaacctc tcatagattt taaaatactt ccttcaaagt cagtaaacat tccaagttac 79741taagctgttg aacaatttct tattttgaag attttttctg ataagttcca aagtgtttct 79801gatgaaggaa ataaaagctg tccagagaaa tctgagtcat tgaggtgagc acagattcca 79861ttggctgctt gtgctttcat cattacagta caattaactt cagaagtcga gagatgctgc 79921agaattttaa gtacccaaag tttctcaaca agttgatttt caagcaaggt cattaactgg 79981gccattgttt ttgccaatcc tccaacttca gccatttgaa tcttgtatga tgaactcact 80041tgctggtaac ctggaatatg ctcctttggt ggttgtgcct gataaaagtc aacaatacgt 80101ttacaatttt ttatcctcaa ttcagaactt ggtattttca ttaagtcacc cagaagcgca 80161actgaactag cagtatctcc agcataagtt atttcatccg atactttctt tttcaaaaat 80221ggcaagccac actattttat gatatcatat gcagattcta caaaatgcgg ctggttttca 80281atttttactg cacacagatt cagaatttca aatgtctgta ctaaatccct taagggaagt 80341tcattctgat aacactgtac cagtttcttg acagatttaa gttgcttttc ttctaagcct 80401tctttagcag tctcttcaaa aagtttgatg acaccattaa ggtccacagg cttcaaaaca 80461ccagttccac atggcgaggg aggccttggg aaacatcatc atgatggaag gcaaagggga 80521agtaaagacc ttcttcatgg tggcaggaaa gagacagcat attgctatgt attttaacac 80581cacttatttc cccccttttt tctttcaata tcatggatta aatagtgtat tttaaatgag 80641tgtgaggctc acttggactc tattccatcc ctttggccaa tttttttcca tttttaatcc 80701aagaatctta aagtttgaat gatsgtcacc ttgttatata ttttattatg tttggaagct 80761tttttgctat tgtctgatcc tcccaacaaa tagccttttg atagaattgc ctttattcgt 80821ttctttatat ctaattctaa cttaagatta gacttaaaag taacttcagc ggttttatta 80881tgaaatattt aaggcatata aatactgcct cttttctcta aatgactgct aatataattt 80941taccaagttc tgttaaagta tcctgttgta cataaagtgc tgttgttttt tatctgcctg 81001ttaacttaga gtgggtttta ttttctttca ctttctgtgg tcgaaggcaa gtatgtctta 81061gtcagtttgg gctgttataa tgaaacactg tagactgggt ggcttaaaca acagaaactt 81121gtttctcacg ttctgaaagc cgggaagtcc ccaatcaggg agccagcagg cctggtgtct 81181ggagaggatg cgcttcctgg tttgcagacg gcaccttctc attgtgtcct cacagggcag 81241agagcaaaaa gaaagggcaa gcgctctcca gcctcttcct ttttcctttt tttttttgtt 81301tgtttgagat ggagtcttgc tctgttgccc agactggagt gcggtggcat gatcttggct 81361caatgcaacc tctgcctcct ggcttcaagt gattctcctg cctcagcctc ctgagtagct 81421gggactacag gtgcctgcca ccatgcctga ctaattttta tgtttttatt agagatgggg 81481tttcatcatg ttggccagac tggttttaga ctcctgacct caagtgatct acctaccttg 81541gcctcccaaa gtgctgggat tacaggcgtg aaccaccgca cccggcctcc agcctcttct 81601tgtgagggca gtaatcccat cataaggggt ccaccctctt gacctaatca cttcgcaaag 81661gtcccacttc caaataccat cacactgggt atttaggctt caccaggtga attggtgggg 81721gaggtgggac acaaacattc agtccacggc aagtttttat ctcaatttgc taaagcctct 81781ctttatttct cctgttacag tgtgataatg ttttttaaaa tatcatttag ctggctgtgg 81841tgacccatgc ctgcagtccc agctactcag gaggctaagg caaaaggatc acatgaacct 81901aggagtttga atccagcctg ggcaacatag aaagaccctg tctcttaaaa aaataaataa 81961ataaataaat aaataaataa ataaataaat aaataaagga aagaggctgg gcacagtggc 82021tcatgcctat aatcccagca ctttgggagg ccgaggcggg cagatcacct gaagtcggga 82081gttcaagacc agcctgacca acatggagaa accccttctc tactaaaaat acaaaattag 82141ccgggtgtag tggcacatgc ctgtaatccc actacttggg aggctgaggc aagagaattg 82201cttgaaccag aaggtggagg ttgtggtgag ccgagatcat gccattgcac tccagcctgg 82261gcaacaagaa Igaaactcca tctcaaaaaa aaaaaaaaaa aaaaaaagaa agaaaagaaa 82321agaagcactt aaagaggctg aggcaggcag atcacttgaa gtcaggagtt caagaccagc 82381ctggccaaca tggtgaaacc tagtctctac taaaaatact agaattagcc agacgtggtg 82441gcacatgcct gtaatcccag ctactcggga ggctgaggca tgagacttgc ttgaacctag 82501gaggtggagg ttgcagtgag ctgagaacat gccattgcac tccagcctgg gcaacagagc 82561aagactccat ctcaagaaaa aagaaagaga gagaaagaga aatatagaca tatgttatta 82621gtcccccagg acctcttcaa aagatctctc ttttcctttc ttatttctaa ttaccatact 82681acataccaca tccattatgt cagaatgtgc cctatttacg tggatttgtc taaagtcatg 82741gaactctgag ttacattccY cgtatttcac agagacacaa tcagagcaca cgctgatctt 82801ccgcctctct tcatttcctc catgtactct gtagctacct gttctcttct ttgttcgtag 82861gaagatctct gttgcgtggt gtgggagttt gtgccagctc atctttttat tgtgctttct 82921gtgagcagct atcaatgcct ccaggtcttc tctttaagaa gaatgtttac tctcaattgt 82981ttaaaaaact aactaaatag gaagaagatt gcaaatccta acctttttgg ggttttatat 83041tgggggcaaa agttggcatc tgcagtaagg attatttcat tttcatctga ataaatgagc 83101agtaataact ttgctgactt gcagggctca ttgtgatgcc aaaggacatt gtaaggtgca 83161tatctgaatt ggaggaggaa gaggggcatc ctctgagcca ggtgtggcag gaggtgacat 83221caaggagggt taggctgcgt aaacccaggg acacaaggag ctgcagtcgg ggaagaacaa 83281ggtctagcgg atggagttgg ggggactgtg tctggagcca atgttctcag atgtgtctcc 83341ggcaggcagg ggcaagggct ggtgcctggt ttacagcatt ccttttggag tctggaatag 83401tggggaaaac tccctgtctt ctctcccctt aggagtctgc actatggaaa caacctgtca 83461atccagctca aggcacacat agcccagaca cccatgagac cctctccgtg gggaccctag 83521agcacctatc atgaacgagg agaccaagta ggtgattttt ctctcccctt cacctcctct 83581tttcccctcc tatctacttc gtcacttttc tcctactgcc aagactggtc cctacccaaa 83641tctgccttac tgggctgccc ccaacacaac aaacctgccc ttgaccatca ttcagcatga 83701gaatgtaatg atcagacaca gaaaatcagg aagagttttg agacagcatc taatctaccc 83761attttagaga tggtatgact gaggcttata taggaaaaag gcttgctcaa ggtcacacta 83821tgagtgtatc agctaggata ggccaggtta cactgtagta catgcaccct ccaaagtccc 83881agtgactcat ggcaacaaaa gtttatttcc tgctcttgct tcctgtctcg tgtggattga 83941ctgggattgc tcctccacat catcctgtct cccacactca ggctaacagg gtagccatga 84001tttgcaatat ttctggtccc tggagtagaa aaaaagaaaa agtactgtca attacacctt 84061ggttcttaaa acaactacag gcatgacatg agtctttttt agcatgtttc attgaccaaa 84121gcaagtttca tggctgtgcc caatttcaag gaggcagaga agtgtaatcc caccaaaggc 84181ctagaaagag gagaaagagt atctgtgaac agccctaatg cctaccacag agaattagta 84241acagagctgg gccatggatc tttcaaccat cgtcctctgt ctccaagatt attccccatg 84301gaaatctcaa tgactgataa tgatgccatg tgctttttct cctgaggatg gttagcctta 84361aagagatttg tgtcatatcc taaggggata gaacactaat gatggaaaca aaggtattgt 84421gttagactgg ctggaggaga aatttgagga tgagaaacat caccagaaat cacaccatcc 84481agycactact tacttccatc tggtgccatg ctgccctgga ccatttcttc atgggtagca 84541tggatgctgg acaccatagc ctggaaaaga agttatgccc taggctatgg ccacatcctt 84601ttgccgagat cacacaaaat ccagggactt agccttgtct ggtgccatct aggcctccaa 84661ggccttggaa atttactggc ctgggaggca agaaacctca gttctcctcc tgcctctgtc 84721atsgacttgc tgtgtgactg tgggcacacc ctttgccctt ccttggccat ggtctttccg 84781tctgcttagt ggaggtggtg atccctgcct gaggatcatg gtgtgagcat gtttcataaa 84841ttctaagact ctctgcctga gtgaggacct attgctatta gcccagttga ggagaaatga 84901ccagtggggc tgacagtaca tccaagtatg tctctactgc atgcccaggg agcagctggg 84961ctctaaaccc caggacgcat agtactggcc tcagcctctg ggaattggga aggctggctt 85021ctgggtggac ctcctactct tttcagagtt agaggtggac tctctggagt tgtgaggata 85081aaataaggta tataaagcac ttagacagtg cctaatacac agtgagtgct ttccaagtgt 85141gtactgctat gaggaaggac atagtaccag aaagtacatc cggaaatgtg atagcatagt 85201ccactagatg atggaccacc atttagttgg ccaatcccca actgttggac atttaggttg 85261tttccaattt ttaataatta taaataatct tgcagtgagc atctttgcaa ttaaatattg 85321tatatacgtt catgatcgtt tgccaaggtt agagtcctag aaatggaatt actgaatcaa 85381aggaactgca cattatttag gcttttgctg tgtaaatgcc ccgtgccctc tattggtact 85441tctagaagga ggtggtttaa gagttccagt tcggccatca tgatggctca cacctgtaat 85501cccagcactt tgggaggccg aggtgggcag atcaccctga ggtcaggagt ttgagcccag 85561cctggccaac atggtgaaac cccatctgta ctaaaaatta caaaaattag ctgggcattg 85621tggtgtgcac ctgtaatccc agctactcgg gaggctgagg cagaagaatc gcttgaaccc 85681gggaggcgga ggctgcagtg agccgagatt gcaccattgc actccagcct gggtgacaga 85741gcgagactct gtctcaaaaa aaaaagagtt ccagttcaga agggaagaat ttgcttagca 85801aaagaggtga tggtctgtgt tagctaggat tagagttcac tgtgagtagc agaaagcact 85861aaacaacagt ggcttaacca gaatggaagt gtatttccct gccatgtaac agaagtccaa 85921agtcagtgag cacagggcag atgggcagct ttaccttcca ccttcctcag tatgggttcc 85981acctcatgat tcaatatggc tgcccaagat ccagccatcc catcaacaat ccagacaaca 86041ggaaggagca aagaagaata gacaacattt ctacttacat tccactggcc aaattacatg 86101gctacatata actgcaaggg agactgggat ataataatct attctagatg accagagaat 86161atttagtagt tctaatactg aggaagaagg gaagaacaga ttttgggaga caactagttg 86221tctctgccat gaggttagct cagactgaga gcagcataaa tggttcatgg aaataagaat 86281gagtaatgca tcctcacggg actgtccaca ggggatgaac aagaccattc cagagagcca 86341gggtgcaggc acaaatggag tagggagggg gtgcagaaga aacagactga tctctggagc 86401tgatccctgg gcagagacat caagactcca tggagaggcc acttccctgc ctattttatg 86461tcKctcaaat gtagggactg gttcttgact gcttgacttc cctagaaact cccttatccc 86521ccaactcacc ttScccagat caccttcccc ctagttcatc tgtaccccac tcacctgccc 86581tcaggtcacc tgccccacaa ctcacctgtt ctccatctaa tcttcctccc agctcccctg 86641tcccccaaat cacctgcccc caatttgctt gtcctccagc tcaccggttc cccagctcat 86701cttccttcca gttcccctgt cccccagctc acctgcctgc cctcagttca cctgtccccc 86761agttcatctt cctcctagct cccctgtccc ctagctcacc tgtgccccag ctcccctgtc 86821ccccagttcc cctgtccccc agctcacctc cccctcagct ctcctgtccc ccagctcatc 86881tgcccccagt ttacctgtcc cctagctcac ctccccccca gtttacctgt cccccagctc 86941acctcccccc agtttgcctg tcccccagct caactccccc caagttcccc tgtccctcag 87001ctcatctgcc cccagtttRc ctgtccccct caactcacct atcttccctg tccctccagg 87061gctggctcct catggacccc gttggcctcc agctcggcaa caagaacctg tggagctgtc 87121ttgtgaggct gctcaccaaa gacccagaat ggctgaacgc caagatgaag ttcttcctcc 87181ccaacacgga cctggattcc aggaacgaga ccttggaccc tgaacagaga gtcatcctgc 87241aactcaacaa gctgcatgtc cagggttcgg acacctggca gtctttcatt cattgtgtgt 87301gcatgcagct ggaggtgcct ctggacctgg aggtgctgct gctgagtact tttggctatg 87361atgatggtaa gggcaggtgt gagagacgca aagcagccgg gccagtacct gcgtcatggg 87421gagccgggag gagcccaggg acccacctaa gtcagagaga gggtgtagcc cagccacgat 87481ctcaactcta tatttctgga cacttcccag aacaaatttc ctaatgcgcc caaatctgct 87541ggggcctgga ggacccctcc ttctacctgg cttcactcga attgctcgaa ttgctgagtg 87601actgcttcca gccacaaaat cccacatagc ccataatgcc cgagagattt atttcttctt 87661ttttggcggg ggtggggggg tgttttttgt ttgttgttgc tcttgttggg ttttgtttgt 87721ttgtttttta tttttgagac aaggtcttgc tctgttgcct aggctggagt gcagtggcac 87781aatcatagct cactacagca tccaactcca ggactcaggt gatccttccc acttcagcct 87841cccaagtagc tgagactaca gtagaactat aggtgcatgc caccacatta ggctaatttt 87901tcattctttt gtagagacag gacatcactg tgttgcccag gctggtcttg aactcctggc 87961cttaagtgat cctcctccct tggcctccca aagtgctggg attatagatg tgagccatca 88021cacctggcta gaggtgtttt tgtttgtttg tttgttttgc aacatggtca gaaacagtga 88081cggggagcgc cttctgccct ctactgatcc gtggggattt cttgggcacc ttctgtttgc 88141acagcactga ggggaaacac aggacattga ggggcaacag cgtttttgga gagaaaatgg 88201gaactcactt aagaacccta cttcaaggca gagtgtggga attgcggggg gcggggcggc 88261gcatgcacat atgtgcagac atatgcagat acacacagtg catatacatg tgggtatgtg 88321cgtaaaccca tgtacacatg catgcaagtg tgcccacatg cataacacat gcatgcctgt 88381gcacacacat acataaactt gcatgcactt gcatatacac acatttctgc ccagcactgc 88441cttcaaagct caccaccaca ttttctccac aacagataac tgagtgttcc atttagttct 88501cccttgctgg gtatctccct gtgagatgag ccctgagcca ggcgccaggc cagagctggt 88561ccccagcatc cctcgacagc cccataccac attatactct cccctctctt gcagggttca 88621ccagccagct gggagctgag gggaaaagcc aacctgaatc tcagctccac catggtgagg 88681actggagttg gggggtggga agggggtggt gagcactgtg aagtccccac ttccaagctg 88741gaggaggctg tggaggctgt ggtggctggt cacagccatt tctcataggc catgctcttc 88801gtctgtccct gacccatttg caagtgtgtc aagcccctaa ctgtgtctcc cagccccacc 88861tcctcctgca gtgactttca aggctttagc ttgtcccgtt accaattctM aagggtctaa 88921cctggaaccc ctcaggaagc ctgttctcac tttaYtgccc gcaacccgca gggcctcatc 88981tcccagcccc aaagtgtcag gcttcatggg gaagccctaa Ycaccccctc ccgacttgta 89041caccttggac ttgacRctca cccacagctg ctgtcttcag cgggggagcc tttggctgat 89101tttctgaagg agctcggaag ggccagtgat taaataatga ccacctactg attcgtgggt 89161cacactttca tctcagcacc ttgatttcta cagaagcatt gactttactt atcgctgtgc 89221atggcagtaa tgccaacaga aaacccaccc tataacctca gctccccagt gaatcaaaac 89281tgtccaagtc cctggtccaa taggcccgca tcactgagct ggcatcatta tctcatggct 89341gtaaccagat ctgattgata tttggccagt gagtcccttt ttaatatagg atttgttaag 89401atattgaagt gctctcgaac tggttccagc ctcctagctg gacaaggagc ccagggccct 89461gctccagggg ctgttcctat tggtcactta gcttagctgt aggaggaccc tgcttgactt 89521cttaaaattc tttcctgagt cctctttcct ttctccctcc cttcctttct gggtcccctt 89581ccttcttgtt ttccttcttc ctttctcaaa ctcttccaga aaggcattaa agtgattgtt 89641gaaaatccat acaatgagac aagactccag aaactattaa gggaagtgag gaaatgtggg 89701tgataagaaa tcgggatgat aaaaccctaa cagaggaggg aggttgcagg cctggccaaa 89761ctcactggga acttcactta tatgaccacc ggcggcttcc atagagcttg gtccttccct 89821gatggacaga tggccattta gtgcttggag ctgagtgttt ctRggggctg ctggctgtYg 89881gccctgcttc ctgcatcctg gactgcaaga ctgccaggcc cagaacatcc accttccaat 89941gggggtgcct agaagtccac tctgcaaata gaagctggtg ttgacccagc ctctgcctgc 90001tgcctgcctt tccagtccct gcatgtgggt gtacttggct gcctgccacc tgtctgtgga 90061ctggaKttcc ccaaaggttc tgggtaaccc ctcagcccag Rtccccagac cccactcctc 90121caccccttcc tttgcttttt caggcctgaa gcgcccacat cagagctgtg ggtcctcacc 90181ccgccggaag cagtgcaaga agcagcagct aggtgggtac cagtgtgggg aggaacataa 90241acagagagat ggggttgcct gggggccaag acccggaccc tggaccttgt cccctgccaa 90301taagcctcca gaggcccagt gactcttgtc tcgcaagttt ccaggagaaa aggactggag 90361gtcttgcaca ggcagaggag aggcctgggc tggcagcgaa ggggctcccc acccatctgg 90421ctgcatgggg acttctctga agagggctcc gctgaaggag ctggaagcac catacaggtc 90481tcagcttcgc tgtccaggct tttccaggga caccctatgt cctcagtgtt gaaggagacc 90541cagggaggag accagtttgg gggtgagcag ggatggggag gggtgaggag gcctgggaga 90601aaccttcaag acagaggccc tgggggccca tggggagaca tggagctgct tagggcaggc 90661caggccagtg agagaagaaa ggggacttct ctcattcttg accagttctc agaatggtcc 90721tcgttctcca cgagttgtcc cactaactag agccttctct gcccccactg ctgccaacca 90781tggctataac cagaggattc acatccgctc aaaatagttc actaccttgg cctttcccca 90841tagggttccc ccaaaagcag ccccagccct ttccctgggt cacctgagac ctctcaatga 90901cccaggaatt aaaaggttaa catctggcac tgtaggggcc cagctcccac agttccgatc 90961ggtaatgcct aggcctacag gttgttagtg ctcacccctg ggcattgcag aactgaaata 91021ttgggaagRg gcactgggga tccagatatt acagaaagag gttgtttcaa taagatattg 91081caaagtagct gggcgcggtg gctcacacct gtaatcccag cactttggag ggctgaggca 91141ggtggatcag ttgagcccag gaattcaata ctagcctggc caacgtgaca aaaccttgtc 91201tctactaaaa atacaaaaat tagctgggtg tggtggtgtg cacctgtagt cccagctact 91261caggaggctg aggcaggagg atcacttgag accaggagtt tgcggctaca gtaagctagg 91321atcgtgccac tgcactggag cctgggcaag actgagactc tgtctctggg aaaaaagaaa 91381aaaaagagat gttgcaaagg aggtgcccta ttcccctccc cccatcttac tgaMccctta 91441caccacgtgt actattgcag aatggataga accgtgaccc acacttcccc attcccacct 91501gcctgtttaa atagctaccg gagagcctat gctcccttag aactgattcc ttctggaagt 91561tggggtggca cagatccccc tatggtgctc ttgacacccc caccctcacc ccatcataca 91621tacacatctg gggctacagg ctcccaacag cccaatactg gggcaagaag acatgagcag 91681agggccgggg ggtcctctcc tcatgccatg tccctgcccc ttgcagagtt ggccaagaag 91741tacctgcagc tcctgcggac ctctgcccag cagcgctaca ggagccaaat ccctgggtca 91801gggcagcccc acgccttcca ccaggtctat gtccctccaa tcctgcgccg ggccacagca 91861tccttagaca ctccggaggg ggccattatg ggggacgtca aggtggaaga tggtgctgac 91921gtgagcatct cggacctctt caacaccagg gttaacaagg gcccgagggt gaccgtgctt 91981ttggggaagg ctggcatggg caagaccacg ctggcccacc ggctctgcca gaagtgggca 92041gagggccatc tgaactgttt ccaggccctg ttcctttttg aattccgcca gctcaacttg 92101atcacgaggt tcctgacacc gtccgagctc ctttttgatc tgtacctgag ccctgaatcg 92161gaccacgaca ctgtcttcca gtacctggag aagaacgctg accaagtcct gctgatcttt 92221gatgggctag atgaggccct ccagcctatg ggtcctgatg gcccaggccc agtcctcacc 92281cttttctccc atctctgcaa tgggaccctc ctgcctggct gccgggtgat ggctacctcc 92341cgtccaggga agctgcctgc ctgcctgcct gcagaggcag ccatggtcca catgttgggc 92401tttgatgggc cacgggtgga agaatatgtg aatcacttct tcagcgccca gccatcgcgg 92461gagggggccc tggtggagtt acagacaaat ggacgtctcc gaagcctgtg tgcggtgccc 92521gcactgtgcc aagtcgcctg tctctgcctc caccatctgc ttcctgacca cgccccaggc 92581cagtctgtgg ccctcctgcc caacatgact cagctctata tgcagatggt gctcgccctc 92641agcccccctg ggcacttgcc cacctcgtcc ctactggacc tgggggaggt ggccctgagg 92701ggcctggaga cagggaaggt tatcttctat gcaaaagata ttgctccacc cttgatagct 92761tttggggcca ctcacagcct gctgacttcc ttctgcgtct gcacaggccc tgggcaccag 92821cagacaggct atgctttcac ccacctcagc ctgcaggagt ttcttgctgc cctgcacctg 92881atggccagcc ccaaggtgaa caaagacaca cttacccagt atgttaccct ccattcccgc 92941tgggtacagc ggaccaaagc tagactgggc ctctcagacc acctccccac cttcctggcg 93001ggcctggcat cctgcacctg ccgccccttc cttagccacc tggcgcaggg caatgaggac 93061tgtgtgggtg ccaagcaggc tgctgtagtg caggtgttga agaagttggc cacccgcaag 93121ctcacagggc caaaggttgt agagctgtgt cactgtgtgg atgagacaca ggagcctgag 93181ctgyccagtc tcaccgcaca aagcctcccc tatcaactgc ccttccacaa tttcccactg 93241acctgcaccg acctggccac cctgaccaac atcctagagc acayggaggc ccccatccac 93301ctggattttg atggctgtcc cctggagccc cactgccctg aggctctggt aggctgtggg 93361cagatagaga atctcaggtg agtaagagtg gaggaggacc ggggagggga ttgggctttt 93421gggggagcag aggccaacca gtctagcaaa cctctgccaa gaggactgga taagaatctt 93481gaatatcaga acataatggc ctgcaatgca agagaggaag ctccatacct tgacaggacc 93541tctggagtcc aaatggatcc tcctcttgtc tgtggtttct ggactctcag tctctctgtc 93601aatcatgggc tgaaaccaca gatccaggct gtctaYagag gctggtggac tttgggcaag 93661tatatggagc acacacatgc acactgtcta ggccagagtt tcttcgttcc taaaacgaat 93721ctgtaacaat agccaactca tatggtcatt gcaagaatga gatgggatgg tgcatgaaaa 93781acattttgta gcacagtgcc tagcacagag caaaggctca gtgtgcatat tagctagaga 93841aaaggctagg ctgcagtaac agagaaacct cctccccaaa tacagtaggt taaataatgt 93901agaattttat ttctctctca tggaagagtc agaggtaggt ggtccaggtc agtggaatgg 93961ctcagctcca cgcagtcatg caggtaccca ggtccctccc attttgctcc attatcccct 94021tgggaatcct atctgcacag tcaaagctac gtcatgggta tatctgtgct ctggcttggg 94081aacaaggaaa gagcatggag gaacatgtaa atgcattaaa gttcagtcct gaaactagga 94141tacatccctt ccactcacat tccattggtg agaacatagt cacctggcca cactgagctg 94201caagggaggc tgggaaatgt gatctctggc tgggaaacca tgttcctcat gatagctcta 94261ttgctgtaga tgaagtggag aatgggcttt ggtggacgat tagaagtctc ggccataata 94321tgtatttgtt agtactactg tattaagccc catctctctg aggggatggc gatgacttct 94381cagctctgcc cagcactgat cctctgacac ttcgcttctt cttatggcag ctttaagagc 94441aggaagtgtg gggatgcctt tgcagaagcc ctctccagga gcttgccgac aatggggagg 94501ctgcagatgc tggggtgagc caggccttgg agctgagaag ggtcttcagc tggggatgcc 94561tggtactctc ctccctctcc ttaatcctac acaaggcaga ttcctggccc cgcccttttc 94621ccctcctcgt tcctccccac catctttgct tactctgtag gttagcagga agtaaaatca 94681ctgcccgagg catcagccac ctggtgaaag ctttgcctct ctgtccacag ctgaaagaag 94741tcaggtgagt gatctccagg agggctcact gactggggag atgagccact gcccaggtcc 94801cagagtcccc ctggggcctg catgttcctc caagtctgac cagtagtttt tcagtgagac 94861ccagagaggg aaggcatcag tccaagtact cagtagggct gagtccaaac ccaaggctcc 94921cagagtcccc agagcaaggc ttccttcatc taatatttta gactgtcttg gaggagcatg 94981agtgttaagg ctccctccct ctttctgaaa tcccaggaga agggatagca gtgcgtaggc 95041aggaatcagg cactgtgggt acacataact acatgaagca ccacacacct tcacatttgt 95101tcacacatgt ctacacttgc acagaagtac acaggcatat gcaagaatat gcacccacac 95161atgcccactc ataggtgtgc acatgtatgc agcgcacaca tgcaccttta actacatgta 95221cacattttga tgccagcaca ctctaactaa ctaagcttgt atgcacaaat atactcctgc 95281cctgtgtctg ggactcagta ccatggggag gagtgggacc agtgaccctc ctccctggca 95341gaacgctgct agtttatatg gcacctttat gacatttaga gaaggcacca cttcatccac 95401acagatgcag cctgtcacta aggttcagca cttgttgagt gaaaataata agttggacca 95461cagtccctat tgtcgtctcg gctgggcatc tgtgaaccgt gattaagcca aggttcagaa 95521gtactgaact tgttgacatt tttcttccct ggctattgaa agtgacttta attttgtctt 95581tcatcaccca ccattgttct taatcaccct cactcccctc aactcatctg tctccccagc 95641taggaaccca ggcaaggctg gcttgtgaac aggtggggaa agtgaggtca gagaggaatg 95701cgcttggctc agggctctgg gtgagtcagg gccacagccg aggctggaag cgatgtctgc 95761agcgtcccag tctagacttt actcattgcc tggggggcct atcccaggca catcccaggc 95821ataagccctg ggagcagggt gctaggtctc ctcattgtgc ctcatgaaga ggccgtcagt 95881ctgtcttcta gatcccttga gccgccaggc aaaaacttcc ccataggaac agaatagacc 95941cagaattgag gccagcagag ggtctttcct cctagccgtg cctgctggaa tccggcgagc 96001aggccatcag ctctttctgg gcccctgtct tatgtctccc acatgagggt ggccatcctg 96061tcacttgcta actggggctt gcaagtgccc caaccccagg gcaaagggac tgggccttgg 96121tctcctcgca gttttcggga caaccagctc agtgaccagg tggtgctgaa cattgtggag 96181gttctccctc acctaccacg gctccggaag cttgagtaag tgatctttcc actgcctctg 96241cagcccagtc cctctctata cctgattcac cccactccct tgcaaggcaa ggaaggtctg 96301gggcccctgg ggtaggggat tccactcctg acacctttgc cacaatcttg cagcctgagc 96361agcaacagca tctgcgtgtc aaccctactc tgcttggcaa gggtggcagt cacgtgtcct 96421accgtcagga tgcttcaggc caggtgagca gaaggaaagg gatcttggcc ttatgggcct 96481tgagaaaaga ggaggggaaa gtgggatggg aaatggagga ggcccctcgt ctgcaggata 96541aatctgtgca taccctactg ttttactcac agctttacct tcagaaccta gctcaggctt 96601ggcacctggt acttggtata tattgaatta atggatggat gaaaagatgg atgtatggat 96661ggatggctga aaagatggat gggtggatga aaagatggat ggatgaatgg atggctgaaa 96721agatggatgg gtggatgaaa agatggatgg atggatggat ggatggatgg ctgaaaagat 96781ggatgggtgg atgaaaagat ggatggatgg atggatggat ggatgaaaag atggatgggt 96841ggatgaaaag atggatggat ggatggatgg atggaaagat tgatgggtgg atgaaaagat 96901gaatggatgg atggatagat gaatggatgg atggatagat ggctagctga aaagatggat 96961aggtgtgtga aaagatggat ggatggatgg atggatggat ggatggatgg atggatggat 97021ggatgaagga atgagtcaac aagtctacga agcacaggca tagccaaagg aataattagc 97081tgtgtgttgc cctccatggt gctgaaccaa tatagccagt tttgatcatg gggtaggtta 97141ggtgtcattt ttcttactca taaaaatttc tattaaaaaa aattcagccg ggctcagtgg 97201ctcatgcctg taattccaac actttggaag gccaaggcaa gaggattact tgagctcagg 97261agttcgagac caacctgggc aacatagtct ctacaaaaaa tgaacaaaat taatggggca 97321tggtggtgca tgcctgtggt tccagctatt tgggaggctg aggtgggaag attgcttgag 97381ccgtggaggt tgaggctgca gtgaggtgtg aacacaccac tgcactccag cctgagtagc 97441agagtgagat ctccatctca aatttaaaaa gaaaggaaaa aaaaaaactc acaacattct 97501taatttttat tatatttctt gtgaaaattg tttttctgac tttttaaaac actcttgcag 97561gattcatcaa gcaaagacat aatcagtatt tcacatgcct gatttaaaac acttttgcta 97621gttccgttat gtttccctgt caattgcaaa atcagcttta tctggatgtt tgaacatttg 97681gggcagaaaa gggtgtgcct gtggtgggtg atgtgctggt ttccagtctc tgggtttcat 97741ggcttggtat ctgatcctgc agggaggcgg acctcatctt ccttctttcc ccgcccacag 97801agacaactgc agagctacaa aggtaagaag ccaagaggcg gtgggcctgg ggccatcctt 97861agaagcaact tgggctcagg cagttgaggg gaaaggtgac caagagacta ggaagagctg 97921gggtatggga attcttcctg ctactgctgc actgaggaat gaattactgc ttaggttggc 97981aaaggatttt aacttcctct agYgtgtcca gaccacatct ggtccctgtg gaaggaagca 98041tccccagaag actcagagga aggcaaaccc ttggggagaa cataaagata ctgttagact 98101ggcattacta gatggtgaaa actctggaaa agcccttaca actgaaaaaa gacagggagg 98161gcaattatgg tacctacatc ttagggttgt tctgagaatt aaatgagtta ctacacttaa 98221ggagtttaga gcactgttgg catgcagtgg

following is a genomic nucleotide sequence of a PROL4 region (SEQ ID NO:2). >12:10841901-10975900 1 tgaggagctt gaccagccct ttaaaccaac tcagcaagtctgaatgatgt gcaaagggac 61 aattagatat gggtaagaag tccccaacct caacccataaaggactgtgt ctgaggttct 121 tacagcatca agggagatgg ataccatggc ttccatcaaggtgctgctag tcctgctctc 181 aaaggctttg gtgaccctga gctctgctca gagcatagatgatggcaaRa ggaggaaatg 241 gggacaatac aatggataac ggggttaaaa tgcatggaaagtactagagt tagattgacc 301 ctttcagaaa ttaagcactt tattttcaga gagtaggtcaataaaagagg aggaaacctt 361 aatttctRtt tttcactgat tgcagatact tctctgaaaatctcggccta agtcttaagt 421 caacgtcttc atgatgtaat tttgccgttc taatgtatatggattttcag ttgttgattc 481 tgtttgtgtg aatcagggca tctaaattga acggaaaatctatatttttc atttctataa 541 tctataatag aattctataa ttctataatt catctagatatcRcatttcc ttgacatcaa 601 actcttgaac atttaaaaat attgataatt ttgattaaatttgtacttaa tcaaaatttt 661 gatgctaagc aggcactatt aaattctttc tattccattccttaatcctt taaaatacag 721 gtcacccctc attcagagtc aatgattggg actagggttctactttggga aactttggtt 781 tattgtatcc aaatctttat aatttttttt ccctaaagctctcttaggca tttttaaatc 841 tcttgtgtct aaacctaagt gtctttctag aaattcacagctggggtttt atcaaatcat 901 gttttaaatt tgtcttcttt ttcatttttt tttttctcttaaaatcctaa gtactgttgc 961 tgaagagttc tttcaagagt aaggtaaacc tttattcagggatgtgttct tttctttgtt 1021 cttcagaagc taccatagtt ttcttttttg tttaataaacattctcaaaa ttctcaaaca 1081 ttctcaaaat tgagtttagg gagtcccaaa gggaaaaagagaattacaaa atctgaaagt 1141 tttagtggtt ctcaataata acattgattg attgagacaagcctcctctg tcacccagat 1201 tggagtgcag tggcatgaac acagctcact gcagcctccacctcctgggc tcaagctatc 1261 ctcctacctc accctcctaa gtagctggga ctataggcacacaccatcac acccagctaa 1321 ttttaaaatt ttttatagag atggagtctc accatgttgcccaggcttgt cttgaactcc 1381 tgggctgaag atatcctccc acctcgtcct cctgaagtactgggactaca ggcatgagcc 1441 accatacttg gcccaataat gttaaatatg gaccatctccttcattttca cattagaatc 1501 tcttgtgtca gagttgtaat tagcatttga acatcagcagaatatagaca cagtacttat 1561 ttgtaaatgc ttttctctaa atctttctac aaccaatggctaatgattga ataacacaaa 1621 tctcccattt ttgcaagagc aataactccc ttgacatataactaatttga agcataaaga 1681 tacataaaat ttgcctacat tattgtagtc aatcagtgaatgtatcattg cactttcttt 1741 tgccactctg aattaaaaca caaatgcttc attctttagttttcaagtcc tgataactgc 1801 ttaaacacta atttgtcaca gagtaagtga aatttaaagccaccattagt cacacacagt 1861 ttacatgaaa aatttgaagt ccattctaat ggcctggggagagactctgt gtaggaggaa 1921 ggatataata catgcccaga gtacatgaga gagctaatgaattatgccac caaagaagtg 1981 gcagaattga gtaagtgagt caaccaacaa ttttgttgttcaaagaaaac ttttatgctt 2041 aatgattttt ttcccctgtt tctgacttgt caatgacccttctactgagt ttatgatgtc 2101 atttcaaact tcattctttt tctcttcttt ctttgaaggaggctttcaag aggaagatga 2161 aaagacctaa ctaccatgtg agctattttg agttcaattaagttattaag aattgttcta 2221 aatggattca acgaacctct gggttactgt tctaataaaacatttctaca agtttttact 2281 gtaatacaaa taatcatgtt taaccatgaa aactttttttctctctttat ttacaaatat 2341 tttctttttt gtacacaaag ttcctggcta ctctaggctggttagtacag ggttgatcac 2401 cctaaaatat ctttgccaca ataYgaatga tgtgcttttgatggtatgtc ccagatacat 2461 atttcctttt gtggcctgac atatggcatt gtctggagaatattcaatat atgcttgaaa 2521 agagaatgtg ttgtctcttc cttttatgga atctttcataaatatcagat caggttgctt 2581 gatagtgttt gttaatgtat tctatattct tacagatgttctgttgttca acaaattact 2641 gaaatagaat cattgaaccc tttaaaaaaa gatgtcttatatacaatcca aacaatattt 2701 ctctgggatg tagagatgat ctacattgag gcaatatatcctttgagagt tttgacattt 2761 tagcataacc atgtattagc ttttgattga atgtcttgtcacagcatctt gataatcatt 2821 gcctggtgca gtccgtggac atacgtcttt tgtggagaaattttcttcaa taaataatta 2881 atagttcaat ttaggcttct tattttatag acattttctacataattttt ctcactttta 2941 cttatagatt gtccttcatt cattgatctg caattctcaaataatctttg aacttactgt 3001 gacaatatat ttagccataa tatatacctt ttctgtgattataagattat tcatagaatt 3061 ccacagcatt tattaagaac tgtcacttta ttgagtctttcccaaaagta gagctttgtt 3121 ggatgatcca agaggaacca tctaacgcgg actatgagaaggaactagtt gcttttcata 3181 aagatgcaaa taacagcaag atgctgttac agttgttttgaattagtcac acaactttgc 3241 atctggaaac acggtttaga actatgaaaa tgccctctactcttgatagt tgatattagg 3301 atattaattt ctatttgctc tgaaacatgt tttaaaaaacagcaactcta aagaaaggaa 3361 gagttacaga agagagaaac agggatacac agacagagtcagaaacagag acagagataa 3421 agagatccag ccagctgtac ataagattcc ctacattacaattacagata cactaattaa 3481 gttttaggag taattaatca cataaaacat taatgcaagaactgaagtta atttagaaac 3541 atcttatctt tgtttgtcct agaaaagcat agtttctcttcagatttgtt tatgttgttg 3601 gaattttctt ccttttagaa tagaYattac tttccaaatcacctttagag atgcatgtct 3661 taatttattg ttccccagta ttaggataaa tgaatgacttgaggggtaKa ttagagctat 3721 ggactcacca aaaatcacag ctaattccgW ctctggcataaagtagctgg aggtggcaat 3781 gagaaaggac aaatagtagg caataaagag gagaaggaaggaaatgacag ctttcagggc 3841 tctcacatgg gcttctgtgc tggggtctct gcaccctgtggcactgagct gcattcgcct 3901 gatatgtctc cgcagggaga ggatcaagag gaaaaaggacattaggcaca cacaaaaggg 3961 gagcagcgtt gccaggttga gaaataactt ggtagaagcatgttgagttt tatttactct 4021 gcaactccaa gttaagtttg ttttcctctt tgccttcacacaaaacctga aatcagcgtt 4081 caaattctca gtggctggaa ggctaataaa cacagagagaaccacgcacc ccagtagaat 4141 ccaggaaatc accctgtcaa ttctccactt catccagaggaaaagtgggt gaaagaaatt 4201 acctatcttg aagaaatagt aaatgctgag gcaggttgcaaaccagatac ttaaatgatt 4261 ggttagtgtc cagaagaagt caatgattct catttctttaccagtggcat agacatctgg 4321 atatagcacc aatataaaac aatctaatag tattacgcacaatagacaaa ttctggatat 4381 ggccagactt gtgaggatta aatcaatgga ggcaattttcctcttcttga cccagtccat 4441 gcagtttacc aatccaatga atgcattccc taagatccccactgaaaact ctccaactgc 4501 taagaacaat aaagtagtct gcactttatc tgccatgtttaaatatgcaa ttagtttcta 4561 gttgacctga tggagtttga catccacacc tgcttcttagattttgatgt agttttcttt 4621 acctctttgt tgtagtctgt tttgtgatgg agactggaatgataaatgaa gactggagct 4681 atcttcatta aatcctaatt agtcttgaca aacaatgtgtttgScgatcc ttggcctggc 4741 cactgtatgc ccatttacct actcagcagg ttgtaattttYcacatacgg ttaatttagg 4801 ctgagctact gtttactaag atgcaaattg ggccaattccctttcatggt cctgcattgt 4861 ctttctgagg ctaaggtgta cgcattttgg attcattaatttgtgatgta tgctagtaac 4921 ctcaataggg ttgctagatt tagcgactaa aaattcaggacactcagtta aacttgaatt 4981 acaggtaaaa aaatcaataa ttttttagtg tatgtccctgattacttaag atcagtgctt 5041 tcaggaagta ctgaagttca aattagattg agtgttccattctggcaacc tgaacatcaa 5101 gaatttggta gcaagtataa aactggtaaa attggaaagttcttgaagaa tttttatttt 5161 gctgaatgtt aatattgata attagtaaag aatccaaagtttggttccat agtgagagtt 5221 atatattttc aggaacttta aaaagttaac actaaatgtaggttatttga ttccaaagaa 5281 actttaggtc attattttgg ctgttcagat gaacaattacatggcatagt gtctcttaat 5341 acaacacatt gtcttactga ctaaatgact tttaatggttatttagcaat gttatatata 5401 ataaggtaga ggacctgtaa taaaaaaaga ctattgattaccttaaattt ttcattttca 5461 cttaaaatgt tctcaaggaa actttgagag gtattagatatgtccattac cttgtttgtg 5521 gtgaagatat catgggtgtt tgaaatttta tacattaaatatatgcggtt ctttttatac 5581 caattatact ttcataaaga tttcaaaaat ttaaaaaatgttctcaaggg tagttgtatg 5641 catgagagag gagatagtaa aattctgtat ttcattctctccactgtcct ccatacaaat 5701 tgcatgagta tctagatcct caacccttct agcaggagactgaagattaa actttccaca 5761 tctctagaga tgaattttta gtttgaaata gcttctctaatttagagtta ctgagaagcg 5821 ctttactaca tggggttcca tagaatagtt cttgggtattcacaccttcc tgggaatgca 5881 tgtcttctct tatccttcct taaaggacaa accatcattccggagatgta gccttaactt 5941 ttggacaatc tgtatgatta tgtagccatg ttcacaggcaactggaacat cacctatgat 6001 aggacgatct tggatatgat ccctgaccta gacttatcatttcttttgtt ttttttttga 6061 gatggaattt cgttcttgtt gctcaggctg gagtgctattttggctcacc gcaacctcca 6121 tctcccaggt tcaagtgatt ctcctgcctg agcctgccaaRtagctggga ttacaggcag 6181 gtgcaaccat gcctggctaa ttttgtattt ttagtagattccgggtttct ccatgttggt 6241 caggctggtc ttgaactccc gacctcaggc gatccgtgtgccttggcctc ccaaagttct 6301 gggattacag gtgtgagcca ctgtgcctgg cctagactcatcatttttac cctacagttt 6361 atcctggtaa atgaagtgct catgggaact atgatgatgtattttctcaa ataattttta 6421 gacttgtttt tgtttagttt tggaaataaa tgtagattttatgaatacca atgttgtata 6481 gcgtgaagtt atgtgaatat atagttaaaa aaaaagttctatgagagagc attttgtcaa 6541 aagcttacta ctttgaaccc cagtcactgt ggtcactagagatgcttgaa acattagaga 6601 ggcaggaaac aaaatatgga agaaaaaaac ttaaccctaatggggtaaaa ccttcccaaa 6661 agtactaatt caataaaacg tttatttcac ttaaaccttaggaaaaatat aaagttcatc 6721 ctagtataaa atctacctgt attttgtaga atacattaatatatttcatc aagagaacat 6781 gaattcttgt gtcttgtaaa acaaagagta acatatacagctagcatatt agaaatatag 6841 ttcataaaat ttatatactt ggggctataa atattagtactttacagagt gagttttaca 6901 taataataag acaaacaact gataattttc cggtaattagtattttagtt ataataaaag 6961 gaaattagta gattgaaata catcagaatt aaacagaaagtaatgggagt aaaagcaata 7021 atgtacagcc atattagtac atacacaacc tgagatatgccagaccaaac gtttcaataa 7081 tattatagtg aggatgKatt tcatgttcac agaaaaattaaaaattttag tgacttattt 7141 tccttttttt tttaataaaa atttatctgt attttttgtaaacattcgtt tcttcttcca 7201 gggacagtat gtgcttattc catggtatac gctattttcttcccaattta tttcttttgt 7261 taagtcctga gtgaaaacct ttgcagggtc tggttagagaaaagaacctg attgagcagt 7321 ctgactaata tttgttatcc ccattctttt atccagattgtgaagagggc ttaggaacac 7381 cccttggtgg gtggcacttg aatgggttcg attcccacctaggtgctgca tgaaaagcaa 7441 gttgtttgag aggaaattta attccaagga aaatcaatagaaatcatcag aataaggtca 7501 accagaaaat attacctagg agaccatgga aatcatcattcgggaaaacc aaagacagac 7561 cagagaacat ctgagttcct aatcaataag ccaaatgggggtgactcagc agagccatga 7621 aaggagcaca attaaggagc ggcaataaaa atgtatgacaaagaaaaatg agaaaccagt 7681 tactgtagct caggtccact ttaccatgag ccaaagtaaaatctaaaaag actttaacta 7741 ggcaatacag gcctaacact tgcgttgagg agacaagaagagaagcatga gctctgaaat 7801 tcttcgagga aagggagtgt aaagatgagc atttctgagttgtaaaggga tagaaaagat 7861 gtattcagta gtttgctact aggtcctaat aatatttaaagatatgcatc caaaataaat 7921 gagataactg gggaaaaatg agaatagact attaagaaaactatattttt tgttagagaa 7981 gatttggttt taaattccat ttatacagct aagatttcatatcaYgcagg caatttttct 8041 acatgtcagc attctgacaa atgtctgcct cagtttattatttaaaacaa ttaaaataag 8101 tgagtgaccc aaggggtaga gaattgctgc aatctctccaaactccacag ctaacttgta 8161 ttttgtcata agatagctaa aggtcatcaa aatagaagaaatatagtata ggaaaaaaaa 8221 gaagataaat gaagtcatag ttttaatggc tctcacatgaacttctgtgc tggggtctct 8281 actgccggta gcatagagtt ttatttgctt ggtatgtctccataaagatc ttactaaaag 8341 gaaaaatgat atcagtgaca caataaatgg gacaattgcaaacaggttaa agagagtYaa 8401 gggttcaaag tatggtattt tactcacatg gaacatttcagtaatgtttc ttttatgttt 8461 ggcaattgca tgaaacctat aatcacaact cagtactattgctgctataa ggctgaccaa 8521 caaggaaatg gcaaagcatc ccagcaggat ccagtgcaccaccatatcaa ttttccactt 8581 cagccagaga aaaagtggat gagaggaact ggctatcttcagaaaataga agacattaag 8641 gcaggtggta atccacatat ttaagtagtt ggcaaatgtccagaaggtaa aaatgactat 8701 ctgttgttta ttttttgtat aaacatctgg gttcagtactattacaatgc catttacaac 8761 cattacactg atcaaacaaa ttctggcgat aactaaattggtaaggatgt agtcaactgt 8821 ggaaatcttt ttcttcttaa tccagtcaat ccagttgactagtgcaatgt atccattccc 8881 caatattcct agtatgaatt ctccagttat taggattataaagatgttat ctgcaggact 8941 gaacatgttt gtagagagaa caatctgatt tcaaatatcactgtagatga gctaatttac 9001 aggtgacctg ttaaagcatg atagatcaat tcttcgaatcatgcaataat gttttcatct 9061 gctgttattt cgacgttgtt attcttccgg aagtgttcagctctcctctg aaataggatt 9121 gtctctacac tcctgaagat gaaaccaact aatgagcagcttgactagtt atttatagcc 9181 tggaaaaatt ataattatta tcctttagtg agtgatacctgaaatgtttg gctgaaattg 9241 ctcccatgca aattcaaaga gattttatat catgaatttgcaacaggctt cttgctgtag 9301 ctctatattt ggatcttcaa attgagtttt gaatagggagtcataaaaaa agagataaat 9361 gtttaataaa aacccaactg tgtaaataaa acttcattttcatcatgtaa gttataaata 9421 agccacaaat cagttctccc agatgcaaag aaattttcactttggaagta attagtacaa 9481 ttagattgta gatatttaac atttagtcag aggataataattcttgtctt catttaaacg 9541 gctaattagg gaaggatata atacaaatct aattagaaatgacttttttt taggcaggca 9601 catgataata tttactaaga atttgctttc ttttcaattgttcctcagaa gtgaaatatg 9661 ccaggtaaag atttgttatc tttagttttt ttttaatgtgacaaataccc ttgaagacaa 9721 ttcatttcct gaatcactgt ctcttgtata ataagcattgtaatctaaaa ttaagaaaat 9781 ttaaatttta cattattcta gttccaatca actccttttataagatttgt aatctcacag 9841 tgcatattat agtcatattt ctagataggg caaacacataaaaaagtaac tacttgggag 9901 actgaggcag ggggatcatt tgaaccggga ggcagaggttgcagtgagcc aagatcgtgc 9961 cactgcactc caacctgggc gatacagtga gactctgtctcaaaataata ataataattt 10021 ttttttttta cataccagta tttccaagga ataggactaattctactatt aaattagatt 10081 tatttgtttt ggggtaaggg aaagtacaac ttagcccaaaatacatttga atgtaggtaa 10141 tacRaatcac aataccctag ggcttcttgt atttaaatagttctacaatg tatacaacac 10201 atgcaggatt ttttctattt cttacatgtg tgtaatgtgtaacaggaaag cataaactta 10261 ataacttaaa gaaacctagt tatctataaa ctactgctggaaacaaaata gaaaagaaaa 10321 tgatgcagag ggaaaaaaat atgtcagaga tttctgagttagaaggctag agagaacatt 10381 ccaRggaatc tttgggaaat gacttctaaa attgccatcaccagctagag tactcatgaa 10441 cacgctgact actgggaccc tcaaccttct ttctcaaggtggttctgcta tacataactg 10501 tgcctcctct ctctgtcaaa ctttcattct tgagattatactccttttcc cacatcatat 10561 atcatcttag atagaatctc attaaagatt gtttttctcccttcttgatc aagacaaacc 10621 cttccagaaa tggagaggaa agggcggtga agataaggaccggccttcat ttcaaagtag 10681 agagttgttt tgcatgattg attttatagc aagggttgatgagttctaaa atcttttccc 10741 caaagcctgt ttgcctgaca ttcttctact cattgagttcaaggcctttg ccagtatttg 10801 tcagaaaaga agtagaacct ctttgtattt agtcaatgacataaaatgat gaaatgttag 10861 tgtgggattt agatgcattt tttttttctg caacgacaaagaatatacgg tgcaatgata 10921 acattagatt atccattaaa caagacaaaa gtgtgaaggaacacaaaaca aatcttcaga 10981 atcatgacag atattaatga cttccaatag catataagagaaaatgaatg caaacaagag 11041 aagggagaat gccttttgtt gatttctttc atccttgtagtcaggatact ctttaggggt 11101 ctctatggaa caaaaRgctt tcttcttcta aRgaaacacttcacaaatct taacatcttc 11161 agaaaagctt ccctcaactt gctatttccc ataattagaatgaatgaatg gcttgatggg 11221 aaaatgacag ttactatgtc accaatcatc aacactaattttccctgagg aatcagagcg 11281 ctagaggtca taacaagaaa gactgggtag tacacgatgaggaggagcag aaagatgatc 11341 actgccttta tggccctcat gtgggcctct gtactggggtctctgaaccc tgtagcatgc 11401 agtcgaatct gcttggtgtg tctaactagg gagaaaagtaacaagaaaaa tgagatcagg 11461 caaaggataa agggaaccat cRcccccagg ttcagggttaactgtttgaa agtacctgga 11521 attttactca ctttgaattt ccaagtaatg ttttcttcatgactgacttt gaaaaggtga 11581 taccacatat catcattctt tggaacacta ataattaaagagataagaaa ggaccccaga 11641 agaatcgcaa gcatgacctt gttgatcttt agcttcagccagaagaaaaa tgggtgcgat 11701 atattggcta tcttgagtaa atagaagata ctgaggcaagaagtaaacca gagacttgaa 11761 ttattggcaa atgtccagac aacattcaca atgcttactagcacgctatt gccatatgta 11821 cctggaaaga gcagcataaa gaagccatct aatgatattacacacagcag acagattctg 11881 gagatggcca agctgatcag gatgatgtca atcaaggaaatatctcttct tttgagccag 11941 tcaatgcagt taactagtac aatgaatcca tttccccaaatccctatggt caattcacca 12001 gcaattaaaa taatatatat tgcctctatt gcacttggcatgccagcaaa gacttgtgat 12061 ttttgaatat cactgatgat gaattgactt tcagctgaccagtgaagaac aatgtatctg 12121 tctgcctctt agaccatgat atagttgtct ttatctctcctttttgttag ctgtgatgca 12181 gatgaagaag atagccagtc ctgctctggg ctagagctgcctaaagactt caatgattgg 12241 ggccagtaat ttcctttggt gcagtgatga ctgaaagcctctaggatgca aatgggaatg 12301 gatctgattt cttaaatttg cacactcttt cttattttaagctctgtata ttttggattt 12361 atcaatctga gctgttgacc aggaacttca agaaggtatcaattacttgg aaaatatggc 12421 tagaatttaa tttgctggat gactatccat ccttgttagtatattgacat agttattgct 12481 attacagatt ctaggtttac tgtgttagct tgtgtggccagtttgcttgc ttggttgact 12541 aaagctttaa acaaattatt attaacatta aatgaggcttaaatggcata actggtggaa 12601 tgtagaggaa agaatctaaa ggtgtaggaa gataggagtattaaggtgaa tttatcatat 12661 gcgaacttca ccttctcccc attatatgac ttaagagggccaaaaggaca ctccattcac 12721 tagggcattg agaaatacat taattaggta attcccaatatcctcaaaaa gctctgcagt 12781 gtttgtcttc tcaactgaag atacaatgag ggaaataaacatgacattgt agtgttgtca 12841 gcaaatgaga cacggaaaat tctggtttag atgttagggtttcttagagt gttcttgctt 12901 tgtacctgaa tgtagcttcc atacatagaa aaaatagtcaaaatactggt cacttatctt 12961 tagaatggaa acagatcagc ctgaacagtg aggaggacaaagagcaagaa agtgatattt 13021 ttggaactaa aaagcgtgat agatatacaa gactcttcagtctgaactga gtgataagaa 13081 tacatgctct gaattttctt agctctattt tttggcagcaggtgtattat aaatattagt 13141 atgctaatta tacacacatt ggaaacacac tgtttcattgggacatattg aaacatgtca 13201 ttgggcatag tagaacatat ttgaaataat agaaaaaggcacacagcaat acacagttcc 13261 cagaaatatt attttgaaac taatattaaa ttttaaggcaatattaaata ttgcttatca 13321 aacaaacaca acaatacaaa gtatttaatg agataatgtgtcatatgttc agggttttag 13381 acaaaataaa gagataaaaa ttaatcttgc agatttttatgagaaagtaa atagctattg 13441 ttattctatt cagtgaaatt tggggtttct ttcatgcttctctgtgaatt ttgtttctga 13501 agaaatatca gattatacaa ctcaaaaata cacactgtgcttgtcatgac caaagccata 13561 aaacaggtaa tattctgaga aggacttcac tagagtaaagaaattgattt gcatgcaact 13621 gacagacaag gtcgcaccat gttcacatct cagggaatgcaatatttcat tattttaatt 13681 aaactttttg aaagataaaa ttttgtggag gggaataaagtaggttcaag gttattattt 13741 ggaaatggaa ctactctggg atcaatttgt aatgtccagaaaagatgaag atgaaaatat 13801 atcatgtagg agggtgatta ataactgaca tattaaaaattcctggtaaa tccagatata 13861 gaatccaagc ttgccttctg aatcatggtc tttcccactacctcacttct tttccttagc 13921 cgtgtggaat actgatcaat ttaagaaagt tatgcctatattcaaatgta aacgaaaata 13981 tgctatagga tatgaccaga gttacctaag ggcctatacttactaaaatg ccaccctctt 14041 ttatctttcc ttgctataaa taaaacccaa atgatctcaatttatttgac accgttgaat 14101 atgttttttt tgtgtgtatt gtgtgtgtgt gtgagtttatgtgttatttg ccctttggat 14161 tttatcttag cattgagaag ttaaagaggg atcctagctgacagacttta acatctattt 14221 tcattgcaga aacactttaa tctagaatgt tagtttattgattatttctg tctctactag 14281 aaaacagatg aaatactggg aaacttatga catcatttcctgtatgtctt tctatgagaa 14341 gtgagcaaac acaaacctac ttaggcagat atttcttaaatttcattttc tttcctttgt 14401 ttaaatactt tttaccattc tttagaacct tacatcttttacaggcattt gtccttgagt 14461 tatgccactg attattactg atcaagagaa atgactatactcttcagatt tataaatcaa 14521 gaacaggata ctgcgatact gtcYtatttt ctgacaacttatattttgaa tagttgctga 14581 ctttatctcc taccccattc taaactcttc actatatccattgggatccc tagtaaacca 14641 ttagcagaag ccatatattc tcatttgttt ctctaacagttcactttatt ttcttcattt 14701 gaactcgagg ttctggaact tttttgttca atggactactttgccagtct ggtgcatgct 14761 ataatttcag aataatgttt ttaagtgcat gaaataaaaatatttgaaat gtataaggaa 14821 tccatatata tatgcctttt tcttgcagta tgaaatatattgttaatata atataggaat 14881 ttatgtgctt tttaattgac ataacaaaaa tatctggcagataaagacta gtggtgaaag 14941 taaatgacaa taggaggtac ctgtaacgac aaaaatttttggcttcctga agtgaaaaaa 15001 ttacagatac tgctggtatg gtatgtgctt gttacttacattcaagacag aagaaatact 15061 agacttaagc taaggattaa taaaaataag tatataatttggtgcccctc ccagttcatt 15121 catcactcat gtccatgaat cttttcattt taatactctacaattaaatc ctgtggcagc 15181 tgttttctct ctcacaccct catagaagtg ggtctaaagctgggatgggt gtttttcttt 15241 ccctctcgta actttcagat tactctactc tctttcctttttcctttttt tttttttttt 15301 ttttgagaca aagtttcact gtgttgctta ggctgttcttgaactcctgg cctctagtga 15361 tcctcctgcc ttggcctccc aaaatgttaa gattacaagtgtgagtcact gtgcctggcc 15421 agattattct ccgtctaatt cctcacaaga catacagcagttcattatct cctgtcatca 15481 ctctatacaa aatattacct ttccttgtca caatcataagccagtcttcc ttccttgatt 15541 tttgctttct acctgaaatt aagtagttaa atatactaaatttcaccacc attctttttg 15601 ctaaagttcc cctatctttt tgttggatga aataatcttctattttttaa aaaaattcat 15661 tcagtaatct tgtttgttgt atttttcWtt ttttaaaatttaattatgga catataatag 15721 ttgtacagat gtatgggggt acatgtgaac ctcatgaaaacagagcaata taaagacccc 15781 tgttgagaag ggataacatg aatgagaaat atgcatgtctctttaagaag aggaacagtc 15841 ttaattaatg gataactata tagagtcaat aattcaagcataaaacactt tattataata 15901 gcatattggg aatagtcttg tctatttgta taaaaatggtgaagaaatag aatatttggg 15961 atccatggag acaaataatt tatttgctaa ataacaaaatttggacaagg cttgaaatat 16021 atataacagt taagagcttc agctctggtg ccagacagctggagttgaag cttattctca 16081 gttttaatga ttgtgtgatt taaattgtgc tgaatcttttctttctccaa tgtgtaaaac 16141 attgatcata gtgctatcta tctcatagaa atattatacttaaataaagt cttgcattta 16201 aaatacttag aatagttctt accatatata ctcaaaaaattttaggtact attattattg 16261 aattttttgg cattccattc aataaatcac gattctcttatatagttgtt ttgctataga 16321 gcctttatta atgtgtctta attacaattt cctatccataatatttggcc aaatttcaca 16381 ttttatgatt gccatagttt gaatgtttgt attttcttcaaaattcatgt ggaaatgtaa 16441 tccctaatgc aacaatatta agaggtgggg ccttttaggagatgattatg atgggtttgc 16501 cattatgatg ggattaaaag gcctgaaggg aactaaataggtcttttaac ctttcccctc 16561 ttctgccatg aaaagataca gcattcaagg ttccatgttggaagcagtga cgaagccctc 16621 accagacacc aaacctgcca ctgccttagt cttggactttctagccttca gcactatgaa 16681 aaaataaatt tctgttattt ataaactacc tcatctcagatactttttat agcagcaaaa 16741 tggactaaga caatgccagt cacatgaaat cataaaatatggcaacataa tagttatggt 16801 ttaacaattt gttttgtttt ccattactca ctgaaaatgtaaacaaactt aaatggactt 16861 agttgttttt ttccttcata tgtagttctt ttctttattcaatattaatg cccagtgcat 16921 ctcataatat taatggacag gtgactattt tgtttctctctgcaatggca taaataaaat 16981 gattttaaca gcatttcaac ttaaacacaa taaattatttatttatattt gacaacacat 17041 ctaaatgttc aggttaagta aaaatattct cagatatataggtttcagac gtttactaaa 17101 catagaactt ttttcaacat tttggaggct acattaacaaaaactataat ggggtgagga 17161 gatacctata tgaatatgtg gagtaaagtt tataagaaattttgtaaggc ttaggatgtc 17221 aaaaaatttt ttaaaaataa gaaaagaaca tttgtatactataaaaatct taaaactcca 17281 gttgtaggta gaagatcaac acataaaact Stgttgtgtttctacacacc agcaatctga 17341 acagtgaata taaaaggtaa attaagaaaa caattccaaaataatgaaat aactaggaat 17401 aaatttcacc aaagaagcaa aatacttata tacaaaaattgatgaaacat tgctgaaaga 17461 cattaaggaa gacacaagta agtagaaaga catcccatgttcatggataa gatgacttga 17521 tactgttaag atatcaatac tacccaaagc aatctaaagattcattaaaa ttcctatcaa 17581 aaatttctac agacttcgtt tcagaaagag gaaaactaatgctcaaattc aggtgaaatt 17641 acaagggaat ccgaataccc aaaacaatct ttaaaaaggagaacaaagtt ggaaaactca 17701 catttaatat ttttatttta tatatatata tatacatatatatatataca tatatatata 17761 tatacacata tatatatata tatatatata tatatatttttttttttttt tccctgtatg 17821 cctcattaag Kcttccctga gccctgggct ggcttcttccatggaagata gcagaagaag 17881 caaggcttgt ccctgttcac agaagatgct agccagatcaggagctgaag atagcaaaag 17941 agggagaagt ccaacccaca gtcccaggcc ctggtgagtaaggtctggtg cgaggagaag 18001 gctgatgata tggaagggtg ctccagtcct gcttcttctgcttggaaatg gagaatccgg 18061 agaacctggg ggatgcaaga tggacaggct gctccttgagaacatccagt ctcctagaaa 18121 aaatgggtgc tctcaaggtg actgaatgaa ctcaactatgccttgttatt ttaaaaccca 18181 ctgtctgctt tctgaattga caatcaagaa tccatgtgtgtcttaaacaa caattgttca 18241 ttgtagtaga acccactggg agaagccttt tggctcaggaactccatcta gccacagctg 18301 attgaattag gggtggatac ctaacccaag tggagccaatcttattctgt gcccactgca 18361 atgtaagatg taaaataggt atttttgtct ccttggatcagtgctgtatc tttagtgtct 18421 agaattctgc ctaccacaca gtaggggcta catgaatatttaatgaacaa atgaatgaat 18481 aaataagtct ctgtgaggaa tttggaattt tgaccttggaaacacagaga atggcagttg 18541 tggaaactga gtcacattgg ctgcataagc tagaaagaacacccactcct tcccatgtct 18601 gaggtcttcg ggtctatctc agtcatgact gagtcctgactctttctaaa gatgattgga 18661 ctcctcttaa ctttaggaca gatggctcaa catcctaactataaaactcc ttcctccttt 18721 cctgcttaca ttcatttctg ttgcaaccaa aaacctttaatgacatgctg agaaattttg 18781 caccttgaca tattctgttc cccaccttag gagcttctctaggagtcata atcactcctg 18841 gctgtacctt gggctgcatc cagatctgcc ccaatgccaccacccccagc tctgtgggct 18901 ctggctctaa gactacaggg acccagaagc aggaaagagtggcatcatgg aattccatgt 18961 cctttagggc aggtgggagg tactatgcct cttctccatccaagaccgct cactacatcc 19021 tgtcctcaga tatggctctt gggaccctca agtcagatactcctaatttt tcatctgctt 19081 ttgctctaca gacagaaaga ggaggtgcca aagaaatctacagattcatt caaatcctta 19141 ttaaaaattc tcacagactt tttttttcag aaacagaaaaattgatcctc aaatccataa 19201 gaaattacaa gaaaccttga atttccaaaa caatcttaaaaatggagaac aaagttggaa 19261 ggctcacact taatatttta aaaattcact acattacttctcagcctttt ggctaagatc 19321 aagtataaaa cttactacaa agctatggta atcaagactgtgttttattg gcataaggat 19381 agacatatag atcaatgtaa tggaattgag agtcttgaagtaagcccata tatctatgac 19441 taattgattt tgacaagtgc gtaaaaattc attagggcataaagagtctt tttgacaaat 19501 gatgctagga caattagata tccacatgca agagaatgaagttggacccc taactcacgt 19561 catatacaaa acttagctca atatggatca atgacctaaatataacaact aaaactaaat 19621 acacctagaa gaaaatttag tggtaatctt tatgaccttggatttggcaa tggattctta 19681 catatgacat gaaaaaagta agccacaaaa gaaaaaatacttaaattgga cttcacgcaa 19741 aataaaactt ttgtgtatca caagccactc caagaaagtgaaaagacgat gtatagactg 19801 ggagaaaata tttgcagatc atatatctgc gaagggcttaaaattcagaa tatgtaaaga 19861 acctctacaa cccaacaaaa aatccaattt taaattgggcaaaagatctc aacggacatt 19921 tctccattga tttacaaatg tctagtaaac acataaaaagacgctaagca tcattagtca 19981 ttggggaaat gcaaaccaaa accacagtaa cRtattacttcacatccact agaatggcta 20041 tagtaaaaat cctggaaaat aacaagggtg ggcaaggatgtggaaaattt ggaactctcg 20101 tacattcttg gtgggaatgt acaacgataa atctactgtggaaaacagtt tggtgactcc 20161 acaagaagct aagcatagga attgctgtat tatccagtaatttcactcct agtatacatt 20221 gaaaacagga actcaaacag atacttatgt gccagtgtatattgcagcat aattcacaat 20281 agcccaaagg tagaaatcac ccaagtgttt ttcaactgatgaatatctaa acaaattgtg 20341 gtgtatacat acaataaaat attattcagt catacaaaagaatgatggtc tgatacatgt 20401 taaaatattg gtgagcctta aaaacatgct tagtaaactaaaccagacat aaaatgagaa 20461 aatctgtgtg attctactca tatgaattgc ctagactaggccaattcata aagccaaaga 20521 ggagatcaga ggtcaccagg aactgtggga aaggaaaaatagggagttac tgcctaatga 20581 gtccagagtt tctatctgtg gtaatgtaaa agttttggacacagaggtga tgcttgcaca 20641 acattgtgaa tgtagttagt gccacagaat tgtacacttaaaatggttaa aatgacaaat 20701 ttgttatata aatgttatca caataaaatt aaaacaaaataaatgtgtaa attaaaaaat 20761 taatattcca gaccataaaa acatgcagca tggaaatgactgtcattaga taagaacagg 20821 ttaaactttt aattttatat gaaatgaaca taaagataccaattttaaac ccagcacatg 20881 aaaatttgat atcaatagaa acaggaaagg aaaaagatctgcaataaagc attaaaatag 20941 agtaaaaaga agaaatgaga agatagaaaa caatgcaatcacaattgtat tgagtgtaaa 21001 aatgtttctc aatagtaatt ttgagattac attattaaataaagtgtcaa aaatgaaata 21061 tttgttttct acaagagaca tttaaaaata aaatgcagaagtgttgaaaa tgcagaggtg 21121 gaaaacacac aatttagaaa tgataaatgc ttgaagtgatggatcctaaa tatcttgact 21181 tttattacac attatataca tgaaacaaaa tatcacatataccttacaaa gttgaacaaa 21241 tattatgtat caaaataaaa taaaataaaa tgtaacaaaattagggaatt taccaagggt 21301 gaaataaaat ttaaagcaaa aattttcatg gcaaacttttttaatatcag taaaaataac 21361 cataaaaaag taatgaccat gaataaaaat gcaattatcaacattgcttc aaattaaatg 21421 caactacaaa taaaataatt atgagcataa agaaataaatcagtaattgt aagtgtaggt 21481 ttactaattt aactattgga aattgtcata tattaaagacgtaaacaagt aatatcatta 21541 atattgttca tctaatgcat atacatttaa ctttaacacatcaaactcat ttaaagaaaa 21601 tcattcacaa aaatggatca tgtattgggc caaatgaatcttatcctagg ccaaatatca 21661 tacacatatg ctgtctgact caaataaaat aagatatcaatacaaaagtt acctttaaaa 21721 atctcattat tgttaaatat ctcttgcatt aaaaggaaagtgtaaagaat attttgaaat 21781 tatttgaatt gaatacaata taagaaccat gtgccaacatttgtggaata catataagtt 21841 gatatttggg cagaaaacat acaattttga atacctttactaaaaaaaaa aagaaagatt 21901 ggacaaagct agtgaaacaa acaacttttg ccttcaaaagtatggcaaac cattatccta 21961 agcaaattaa cacagaaacc gaaaaccaac tatcacRtgctcttgcttat aagcgggagc 22021 taaacattgg ttcacacaga cgtaaaaatg ggaacaatagacactggcga ctaagtggag 22081 agggagggat ggcaggggtc agggggcaag gactgtcaaactacctattg agtaccatgc 22141 tcactacctg ggtgatgggt tcaattatac cccaaacctcagcatcaagg aatataacct 22201 actaacaaac ctgcacctgt accccttaat ctaaaatagaagttaaaatt atttttataa 22261 attaaaaaaa tctgtataaa aatatatgaa aaaccagatgtctggaaaaa tccttccagt 22321 caggaaaaca taaagatact gaaaaagaag ccttcagaaatatttttaaa gtgtatggtc 22381 cagctgtcaa gagagttaaa aaagaatcct cagatgccaaaaatagtagg aaagtgcaaa 22441 tcatgagagg aaacagtgct gaagatagag ttagtccctaactaactatc ttggagttgg 22501 atagaggagt ttatcgagga gacggcattg tcactctcctttaaaccagt gtgtaagtat 22561 tttttaattt tgttatgttt ttaaattgat gttccgcagtgaaaaggtat atgcaaaact 22621 atccctaaag gctgagggag ctgagaggcc aaagaaagagtctaacaatt ccagtttctc 22681 aaaaagaaat atttattgat ttatttataa tttttacttcaacaactttt tgggtacaag 22741 tgtttttttg tcacgtggat gagttatata gtggtgaattctgagatttt agtgcaccca 22801 tcacctgagt agtgtaaatt gtacctaata tgtagcttttttatccctat cctcccttcc 22861 Rgcctccccc ttttgagtct ctaaagacca tgatatcactctgtatgtct ttgtacactc 22921 atagcttagc tcccgcttat aactgaaaca tacagttttggttttccagc catgtgtgtt 22981 acttcactta gaataatggc ctccaggtct atccaagttgctgcaaaaga tatatatatc 23041 ttatacatat atatatctat cttatacata tatatatcttatacatatat atatcttata 23101 catatatatc ttatacatat atatacacac acaatcttttttaaaagaaa gcaatattta 23161 ataggaactt ataaacagag gcaatgtctt gggtggccgcaagatattgg atccccacag 23221 ccgccctcca gaaaatatcc tttatatatt aagctttttgggtaaaacat gtgcagctct 23281 tcatacctca ggctctcttg ctaaaactta tgaccattaagaaggttaga taagcatctt 23341 tatgaggggc tctctatgtc acaggaatta tttaaagaacttgctgcaga acacattggc 23401 atgcaggagt taaacatcag tcgttgtggt agtttcgcttcaggatggca tcactctttc 23461 tacgcaataR gctattttcc tacgttatat aataattactgtacacattt atcaRgtaca 23521 tagtgatgtt ttgaaacata caatgtatag tgattcaataggttaataag aagatctatc 23581 atcttaaacg tttatcattt ctttgttggg aatattaaaatcctccttcc tggtatttaa 23641 aactatgtat tattgttagc tatagtcatc ctatagtcctataaaacact agaacgtatt 23701 cctcctatct agctgtattt tgtatacttt tacaaacccctatccttccc agcctctggt 23761 atactctgct cttcttttta cttctatgag atcttctttttttagtttcc ttatatgagc 23821 gagaatatgt ggcgcttaac tttttgtttc tggcttatttcaRgtaccat aatgtcttcc 23881 tttccatcca tgttgtagca aatgacattt cattttttaaaaaaaactgt tatggctaaa 23941 cagtattcca ttgtgtacat atacagcatt ttcttcatccattcataaac cagtgcttat 24001 gttttaaaag accatatgca tgttggataa gatacctggtttggacttct ccaaagttag 24061 aagtgagatt ggatactatc aacaagccaa gatccctgaaaggcaaaMcc ttaataggca 24121 aatgagaaaa taatataYgt ccaacaaaag gctaaagtaggaatacttaa cactcagcca 24181 gggcagtgaa ttgaaagggg aatgtaaagg ggaatgaaagaggaatgttg ctctaagaat 24241 ttgttataat agcctttcac tcatgtatat ttggggctagaagttgaatt ctacatagcc 24301 taaatcatta caaactgaaa agtttagctt aaagaagttgatactggaag tgatcccagt 24361 cagcctttac aaagtcaaac tttttctggc agacgtcactgtaaacctga tctcaaagaa 24421 ttcccacaga taatattcta ataaaactga gttcaaaataaaatcataaa acatgtgagg 24481 accaaaccaa tataattgaa agttagtaca aacaacaaagttagtaatca gagctacaaa 24541 ggcagcagat atcagaatta gagcttctgc agtctgtgcaaatgctaagg aaggttcttt 24601 ctctttccct tgtttctcaa agcaagtatg actaaacagatattttttca caggcctcat 24661 cttttattta attatatttt tcatatgaca taaattattgcatagaaaat tacataatac 24721 tacaagaaaa taaaaattat ctataatccc aatatctacaggtaaagaac ctagacatcc 24781 agtttatgta taaatctaat caaaacagat tttttaagtaacatgcttat tatctgtcat 24841 ctatctatgt tgagatctat tttacaacgt atgtagaaacatgttaacaa tggttagttg 24901 agtactgcaa atgtgcataa tcaatttctt ctttatatatttttgtgttt gctaatttta 24961 gatgaaactg tattttatag tacaaaatgg agcttttgatgaaaggaaca ggtatcagta 25021 aaaataagct aatattggca aaaatagcag aagaaaacataaatcaatat gagatcatgt 25081 aaattaccca cctattgccc caaacattac aaactagttacttactaaac agcaaattaa 25141 gaaaaaatca taacacatat atggcatagt gttaatgtataaactagtca caaaattggt 25201 aagaaaaact ataagacatg aaaaaataag gagaaaacaaatgaacaatt cccaaaaagt 25261 atatgctatt ggttaatccc gtagtaatgc ttaacttcactagtaatcaa atatatgtga 25321 agtaaagata tttttgcaat caaagtagtg acattaaaaataataacctg tttgattttg 25381 agactgcaac aggacggata ttttttcact attaacatataggtttatta tatctacaga 25441 gcaatttatc aattctgcaa ggaatttatc aagtttctccccattggaat tttccttaaa 25501 aataatttac atactgctgt ctctatatat tagtcacactggacctttag tttctggact 25561 accctctact ccttcttgct tcaaggcctt gacatgatggtttcccaccc caaatttctc 25621 ctcttttgtc caccatactc ctacacatta ttaagatatcaaattaaacc atcttaacca 25681 cagtgacatc ttgtccattc tcatgaccag gtcaggtcagcatcctatag tttttctctc 25741 ccaatatcat aaatatcatt gtttaagtta gctagctattcatttgtttt ctattagaat 25801 atattatcca ttatattggt atgttgttca ctactatatcctcaatacct agccaagtta 25861 gcattcaaat agtattcata tgaatgaata gtattcactctctataatac tcatattatg 25921 tttatttcta aaagtggaaa actgaagaca acttaaacgtaaacgatatg ataaacacta 25981 agtatattat tgtatatttg caaggggagt tattatgtaccccttaaaaa gattattaca 26041 aataattttt gaaaacatgg gaaaatgaat aaattctacataatcaacat aatctataca 26101 gagaacatag ttctttatac aatataattt caaatgagccaacctggtat atttggttga 26161 aataggcagc agcattaggt ttttttttct gttatctcttaactatctgt acattctaaa 26221 cacaatagtt taaaaatata aaaatattga tttggaaacctgacatagaa aattaattaa 26281 gtccttatat ttggaaatat tccactcatt aggccataagtatgagatca aggcactttc 26341 ggaagttgcc aaaataagtc actgagttta taacctgggttggacataaa agttaacttt 26401 aaaactcaaa atttgaaacg tttctgtgag tgcaagtttgtgtatatgaa tgtgtgtgtg 26461 tgcatgttYa tgtgtgtgtg tgtatgaagc ctgaagacagaacatttcat atttcaccca 26521 gattctatcc tgtgttcatc ttgtgtcctt tggtcagggactgtgttgaa tcttgcagcc 26581 tttggatcga tgcttctaga aaaatcttta ctcaactttaattggttcat aacctgcctt 26641 atctcaaatc actgattttg aagagcatat ttatttcatcagtgttttga tcccatatcc 26701 ctcaactaga cccttgtttc cctaactata tttcacagaactacatctac tcctctgact 26761 acttgctaca catcgtacac cagtaaaaaa ggatgttagattctaaaaaa tgaaaaaaaa 26821 acctttaaat aattaaaata gcattctgta aagaagtaccaccatctatc ctcacttcca 26881 tgttctaaat gtcaagattg atctaatttt ctttctttcttttttttcct ttgggttttc 26941 tgggagaatg ggtgtccttg cttcagaaaa gtcaggtaaagcacaacaag aagttttatt 27001 ttagagctta ttctcaccgt tataatcttt acagcgaatagtctccaggc tgatggtctt 27061 cctctaggaa gtttgtagga agaagttgca gggtggggaagctgactaag ccactgaatt 27121 tgcatgtgag attaaaatct cctcaaagac tgagattccctgaaacatag tcaaaacaat 27181 tatggaagat ttcagtgatc tttaatgaca cagttaaaaagacaatgcaa gaatgcagtg 27241 caatgaaaca gatcttcaag gatttattca ctggattcctttcgtggaac atccatctct 27301 tcccaaggtg tctatgtgac tctgagattt ttccttttctcacagcactt caattgctgc 27361 agtaccctca aagaggcttg ctttagcttg ctgtttcctagaattaagat aaatgagtga 27421 ccccagggat agatggctgt ggttgtcatt ccaaacataagcagcagttt gttttctcgc 27481 acagtaaaac atgatatttc tatggccatg cctataaaatacaagataaa gaggatgatg 27541 aaagatatca aaactttcat tgccttcaca tgagcttctgtgttggagtc tctcaatcct 27601 gtcacattcg attgcatctg cctgttgtgt ctccaaagggaaatgattaa aaaaatacat 27661 gtaattaggg atagtgtaaa gaagaaaatg actcccagatttagcaaaat ctgtttaata 27721 aagtattcac ttttatacat gttgagatcc cagactgtgtcattcttcRt tttataatca 27781 ttaagaatct tcgcaatgta tgcaaaatta agtaacgatgaaataagtaa gaatactatc 27841 atgaagggaa gaaccatatt tgttctgctc ttcaaccagagaaatatgta gttggaaaaa 27901 tttgctatct tcaggaaata gaagatgctg aggctggtggcaaaccacat acttgattga 27961 ttaccaatta cccaaaagta actaatatat tcaattaggttaccggaggc atatatattt 28021 ggagagaata tctgtataaa tccatctgta attattatccatatcagaaa aattcttgaa 28081 atagctaagc cggtgagaat aaagccaatc gtagataacttattcttggc acagtcaatg 28141 cagtttacaa gtccaataaa tccattcccc aaaaccccaaacactgactc actaactaca 28201 acaaaaatga agatgccttc cactacacgt agcatatctgctaattctta atattgcttc 28261 tgttacatct atcttagatt acctgctgca gaatgaggcatatattggct gctcgacgga 28321 agtgtgactt tcttctcttt cattatataa agacttgaaattgccccagt aatggcttga 28381 gatggaatca gaaatttctt aatgaagacc tttccaaatatgtctacaaa acatagctag 28441 tctggagaat gtatgttcag actgaatgcc tatctataatttgttaaaat gcaaatgaaa 28501 gtttctgttt catggatttt acatgtcctc ctgagactattttgcattta ataaattgaa 28561 ttgtggagga ggagtgccaa acatatacct atagaatgtaatgcaaaaca gaaacaaaaa 28621 ttttgacttt atcttttaaa atatcaaatc attatttatgtcatttataa tcaatttata 28681 tatgtacaga aaatactata tagaaatgaa aaacaaatttagccactgta gatacttact 28741 ctaaatgatc gatttaattt ttaattaaaa tttaagctcaactctaggta tgacaagttt 28801 cacaaatatg tcacaaatct gttgctgttg ttttatacttatggttatta cacccttgaa 28861 tgcagaagat tatgttgact acccttgaca tgaaaggctcttaggaataa tctcttcatt 28921 taacacatta ctgtttgctg gcacccattc atttatccaacaagtattta ctgaatgtct 28981 agatacaaca agctctcttt tgatctttga gggaatatagtggtagacag aatttcaaaa 29041 cttgtgtgat ttatggagtg tatattctaa tgagggaggtgggaaacaat taagtaaatc 29101 aacatactga tctaagaatt aaaagtgaaa ttaaaagtgaaatctaacat ttattgagct 29161 tttattatat tcctagcgct atgttaagta tctttttctcatcttaattt cacaagcctt 29221 caatccttta aaagaggggc tatttttgta tttacattaaatatgaataa actaagcttt 29281 ggagaaaaga agaccttctc tcacttatcc tggcatgactaccataaata aaattgcaca 29341 atgatttcaa aatgaagtta aattaagctt aggaatagactacaaatgat agcaaaacaa 29401 aacaaaccaa ccaaaagcaa gtagaaaact taaattctcatgggctgcac atactgaaat 29461 tgacagataa gaatatgtat agataaatta gctaatgtatacactgttta caaaaaacaa 29521 gatgcaattt cagagaagag cagcaagaga ctgttagaaagaaattggaa catttgggga 29581 aaaaacttct agaaatggag aatataatta tataaaatgtaaatgaacta aatactccaa 29641 gtaaaaaatt aatagtaaca aagaagaatt taaaactctgacYgtatact gcttacaaga 29701 aacacaattt aaatatagaa aaggaaaact ttaaagtaaaattatggaaa aataagccat 29761 gcaaacatca tccaaaagaa atctggcata attataacaatattctataa agcagacttt 29821 aaggtaagaa gcagcattag agaagaaatg gggagtgtactttatagtga taaaaatgct 29881 caaatctaca gaaaggtagg gtaatcccaa aaatttatgaaccaaataat ctaccaaaca 29941 tttacagaaa aggaaaaata agacaaatct acaatcacaatgaaagactg taaaatacat 30001 aactcaataa ctcatcttag aaacaccaaa aatcaatagatggaaaatac atgaaaaaac 30061 aattgacaaa cttgtgttca acggtaaaat aattcatattcttttcaagt gcacatagac 30121 tatttttacc aagactgatc atgaatccaa aagaatatctcacaagtgtt gaatgttctc 30181 tgaattcaaa ggaataaaat gaagttcaat aataaaaagataactagaga aagaaacaat 30241 ttactaagac aaaaaacctg taaatactct tgagtcaaagaagaaataac aaaaattaca 30301 aaaatatttt taagtaaata atacaggttg tgtatcccttatctgaaatg tgtgagacca 30361 gagagctttg gatttcggat ttttttttct ttttttttaagttttggaac atttgcatca 30421 tactgattaa gcatctcgaa ttcaaaaatt tgaaatccaaaatgttccaa tgagcatatc 30481 ctttgagcgt Matgtcagcg ctcaaagagt ttcagattttggagcatttc aaatttcaga 30541 tttttggatt agggatactc aacgtgtaat aaagaaatgacctattaaaa tttacataat 30601 ccaattaaag ccattctttg aaggaaattt gtagccttgaatgcatcttt tagaaaggca 30661 gggtaaaaac agatgaattt tgttttcatc tcaagaagctagaaagctaa gagtgaaaga 30721 aaatagaagt caagaaatta tagtWacagc agaaatcaccgatagaaaag aattaggtac 30781 agtaatatac taatgttggt caaaagaaaa tacaaattactacagtcctt agagatatca 30841 gaaatatggc ctgggtgtgg tagctaatgc ctgtcatttcagcactttgg gaggcccagg 30901 tgtgagaatc acttgagtcc aggagtttga gaccagcctgggaaacatag caagacctta 30961 tctctactaa aaaaaataaa taaataaaaa taatttagcagggcatggtg gtgtgcacct 31021 gtagtcccag ctacttggga ggccgagcag gaggattgcctgagcccagg agtttgaact 31081 tgcagtgagc tataattggg ccactgcact ccagcctgggtgacagagtg agacctcatc 31141 tctctctctc tctttctctg tctctctctc tctctctctatacatacata tatatacata 31201 tatatatata catatacata tatatatata tataatactatataatatag ttacatatcc 31261 ttgtatataa tcttttatta taagacaata ctgtgaacaactttatacaa aatttcatca 31321 agaatactta taagactatt acaggctaat atagcctgaacatagatgca aaaattccaa 31381 acaaaataga gtccagcgat atataaaaga ataattcattataacaaaYg gagttttaaa 31441 agccaattgt gagattaaaa tgaaataatt gaagatacagtcaattaaca caaaagaaag 31501 ccagaaaaag gacagaggga caaaaataag tgagataaatgaaaaacatt gagcaaaatg 31561 caagacttac agccaaccat actgataatt acattaaacatgatttgact aaacactctg 31621 atgaaaagat agagatcatc agaatgaata aatgaaagcacaattaaatg ttatgcctag 31681 aagataaaac attaaacata aaggcacaga tagattgaatgtaaaggaaa gaaaaagcta 31741 tactatggaa tttgtaacca tagaaagcag gactggttStattctcaaag tggctatatt 31801 atcaagacaa gtagtagaaa aatagaggga agttgtataatgataaaata ttcaatttat 31861 caaaaatact taacaatcct gaaggtgtat cagactaataattgtgactc aaaataaata 31921 aagcaaaatt aacagaacta aagcaagtga taggcatagcaaagatttaa aaaccccttt 31981 gctgtgatct gcatgtgtct cctccaaagt ctatatgttgaaacttaatt gccagtgtgc 32041 cagtattaag agatggttcc tttaggaggt aatgagggcagacctctcat gaatgagatt 32101 aatgaccatt ttgatgtgca ttctaggtat gttaactctacatgccttag attgtgcaat 32161 agtaactggg ctcttatgaa agggttttaa caaMttccacaagttctaga ctaatttctg 32221 cagttacatc agcagcaaaa atattcacaa aaacaatggtgccactgcat ttcagcctgg 32281 gcgacagagt gagactccat ctcaaaacaa acaaacaacaaacaaaaaac ataaagtaac 32341 tggctaaatc catattatta cagagacaag attgttacctagaggaaata agtggttttc 32401 attctatttt gcttacttct gttaaatttc taacatccacYttctgtaga tctRaatgRg 32461 tggattacaa cttattcaca cctctttttt atatataggtgattattttc tctttaaaat 32521 attttaaaaa ctattttaga ggtcagtcct ggcccaggaagaatttattt attttatttt 32581 atttatttat ttatttattt atttatttat ttatttatttatttatttat ttttgagaca 32641 gagtctctct ctgtcgccca ggctggaatg cagtggcgcgatctcggctc actgcaagct 32701 acacctcctg agttcacgcc attctcctgc ctcagcctccccagtagctg gaactatggg 32761 cacccgccac cacgcctggc taattttttg tatttttagtagagacgggg tttcaccgtg 32821 ctagccagga tggtctcgat cttctgacct cgttatccgcccacctcggc ctcccaaagt 32881 gctgggatta caggcctgag cccgcacccg gccaggcccaggaagaattt aaatggtact 32941 tactcagtga cacaggacat atacaaaaaa agagtacgatgaagcaagaa agattacacc 33001 aagacttctt gttgacttct ctctgaggcc tacctgtgtagctaagagtg cccgtccttt 33061 caggccacat ttcaggtgcc agagaatccc caagaaagttttcttcattt ggccgtatcc 33121 aaaaatcaca acatatgagt gacccaaaga atactgacttcatatcataa accagatatc 33181 acaaccagtt tgttctgtag caaaaaatac ccctgtaaatgtcaagaaaa gggcaaattt 33241 ttatagagca acaagacaaa aaaggaaaac cgttttcttggctctgaaat gggcccgtat 33301 actggggttc tgcaatccct gaaattgaac ctaatgtgtttcatgtgtct tcacgaagaa 33361 aggagtaaca acaaaaagaa aatcgaagaa atagagaaagaagagagaga tccaataaag 33421 aagattatta tattattaac atttttatta ttacttacattgagtaatcc agtcatatgt 33481 ctttcatatt tttttggaca cgataccaga agacatcaaaagtgtacgtc aaaggaaagt 33541 tggtaaacag aaaaggcaga taccagtgga agtatgaaagctaccttgtt aattctccat 33601 tttagccaaa agtaaaagag ataggagaaa ttgacgatctttaggaaata aaagacagca 33661 agacaagtag caaatcaagt gcacaagtgg ttggccagtctccggacaat ggtaaggata 33721 tggtttcttt ccctagcatc agggattttc taatagaagaatattgattc tggctaaggc 33781 caaactagta agaatgaagc caaccatgca taacttctgatttctcatcc aggcaatgca 33841 atctgtgagt ccaatgaatt aatttcctac attccttttataaatcccct acctattaca 33901 gtcatgaaaa cctccaatat attcaacatg gctacaaagcgaaaattctg atctgcagtt 33961 tgttgtgtga ctaattttca gatgacttac tggataataacatagatctt atgtttgatt 34021 gtgcagtgtt tttattttat catggctttt ctttttagctataaagttta gcaaactgca 34081 gtaagacagt gaaatcactc tctgtaacat tcattgaaaatttctaacta cctctgctaa 34141 gaatttccag tcttggtcca gctgtcagct acaaactgtaaatttcttta tagaaagatg 34201 atcacactct ggggactgtt gtggggtggg gggagggggagggatagcat cgggagatat 34261 acctaatgct agatgacgag ttagtgggtg cagtgcaccagcatggcaca tgtatacata 34321 tgtaactaac ctgcacaatg tgcacatgta ccctaaaacttaaagtataa tttaaaaaat 34381 taaataaaaa taaaaaaata aaaacaaaat taaaaaaaaagaaagatgat tgacaaacca 34441 aattatcatt tgctaacatg taaataaaga cacagactaattttcattgt tttgaataaa 34501 attagttctc ctcttccaat gaagtgtttc atttgatttacaaatctgaa gtttgaaaca 34561 gaaacctttc accacattaa aattattcaa catgtagggaaaaccagcaa tccttatgct 34621 aattaattat aatgcagtca cagtgtagat tgctacattacaaagtaaaa tacagagctt 34681 gaaattttca agaaacttgg tgggcaatat atgaatctaatatgagtatc ataattatca 34741 ggaaaacttc ccccaccgct ccccaccccc cactataaagatgcaggcct ttcaaataat 34801 ctgtcagtag cattttaaac acatttttaa aaatgatgtctttcagtgaa acataagcac 34861 cccaaactaa acatggaaaa agtgaacttc ttttgtttcctcaaaaacaa gattctagca 34921 ctttgggagg ctgaggcagg cggattgctt gggcccaggagtttgagacc agcttgggca 34981 acgtagcaaa accccgactc taccaaaaat acaaaaattagctaggttag gtgtgttggc 35041 acacgcctct gatcccagat acacaggagg tggaggtgggaggatcacct gagcccaggg 35101 aggttgaggc tgtagtgagc cacgaaaaaa aaaatgttttttttgagata Waagagtctt 35161 caaatatttc cacagaactt tgttaaggtt acattgtcttgaggggaaaa tgcttaaaaa 35221 gtcatgaata tacttaggat gatctgttat gtgtatatccatatacacag atataggtac 35281 atatatggct acatatacat aaacatatac atgtatacaaatacacacag acacatctat 35341 ttatacacac attgtactgt gWtttgtcct ctacttctctgtattttagc ttgtctggtt 35401 tttgttactt ggattgcttc tttttttgca ttattttattatttttaata aacgcaataa 35461 ttgtacatat ttatgtgata cagtgtgata tgtgaatacatttatacaat gtgtaatgat 35521 caaatcaagg caattagcat atccgtttct caaatatttaacatttcttc gtgttgggca 35581 cattcaaaat ttgtttttct agctcttgta aaatatacaataaattgttS taattgtagt 35641 caccctactg tgctatagaa cactagaact tgttccttctacctggctat acttttgtat 35701 tcattaacca acctttggct atctccctct cccttctcccctatcacatc tctaggaacc 35761 attattctgc ttcctacttc tacggaatca actttattagcttccacata tgtgtgaaca 35821 tgtcatgttt gtctttctgt atctggctta tttcacttaacagatgttct ccagtctcat 35881 ggatgcttcc ataaatgacc aaatttcctt cttttaatggctaaatagta ttccattcat 35941 tatgtgtata tatcatgttt tctaatccat tcaaatgttaatggacactt aggttgattt 36001 catgtctcgg ctattatgaa tggtgttgca aaacacatgggagtgcagat atcacttcga 36061 catattgatt tcctttcctt tggatacata cccagtggtgggattgctga atcatatggt 36121 agttatattt ttagttttta agaacttcca ttctgttctccataatgact aaactaattt 36181 atatttgtac caacagtgtg taaaacttcc tcctccacatcctcacaagc atttgtgttt 36241 ttttgtcttt ttgaaaatag ccattataac tagaataaaataatatctca ttgaggtttt 36301 tatttgcatt tctctgatga ttagttatat tgagcacctttttatatacc tattggccat 36361 ttgtatgtct tcttttgaga gatatatata cagttaatttgcccatgttt taatctaatt 36421 atttgtgttt ttgactgagt tccttctata gtctggatatgaattccttg ataaatgaat 36481 agttttcaaa tattttctct tattctagag gttgtatcgtcagtctgtcg attgtatatt 36541 tgtgtatgct tttatcaaat caacataatg taaactaaatgagataaagc taaaatggtc 36601 atcgcttgct taacataaaa tgcattattc atctaattttattgttacta ttatataata 36661 ctataatttt aattttgcca ttgattatat agttgcaattctgatggaaa cagtgaaatc 36721 actttctttt aacttatttc aggcagataa tttcatcaatgaggctcaat gatctatcac 36781 caaacataat aaaagaagtt ggattaaatg aaagggatgtgtgctgagtt atttgttgtc 36841 ttcatttttg agagaattac caatcaattc aggtgtgtttatgaggattt agatttcctt 36901 atattcatgt aacaccgaag gttcctcagS acacagagagaagcctgttt cagtttgctg 36961 ttccataaaa tcagaaaaaa atgaatgact gaatagatagaaaaatgcta actcaagagc 37021 aaaaatctgt gccacaatgc tatctagatt aaaataatttcgctttataa tcgtattgct 37081 caaatagtat ataataaaga ggagcaggaa tgaaattatagatattaggg gatgtcaaac 37141 ttatccttgc attcttattg tacaaaattt cctcagtttacatttaagcc cacatgtaaa 37201 attacagcag aatattctgg aaattgccca acaggtgagaataaaatcta tcagggagag 37261 tttccagcgc ctgatccaat caatgcagtg aaccagcaccataactctac ttctctgaat 37321 ccccattatg aaatctatag cagaaatcat tgtaagcatattcttcaaca tgtttgccat 37381 gcttgattat gggatctctg ctttctgtac accactgatttataaataag atgttataaa 37441 tttgtgtatc caaagcttta gtgttcaata tgtttctttatcctgtgctR ttgattgaca 37501 ctggaaaaga atggaatgcc tccagtgtgc tcccagacaaagtcctagtt gtctttgaaa 37561 aacgaaaact acctttattt ttctcagaat ttcagctagtgattttcaag gcagatatta 37621 tattacctgc tgttgtgaca ctgtttataa tttccttgtaaagctatcaa ctaggatttg 37681 aattctgatt tgttaaaata caaaatagag attttaaaatattttttgca tctttttttc 37741 cttaagattt aaatgttttt tattcattca ccttagttataagctagaaa cattaaaata 37801 tggtgtcttc atggatctcc tgagaagcaa ggcagtcattgggataaatg cctatatgag 37861 aaagcaagga gagagcgaaa ggtgaacaga gctattgtactaggatataa ctttgacccc 37921 tgtgaaggag agagagaggg aaagaaggaa ggtcttaggcagcagtgcag tcctgagaac 37981 atttcaagat gtccagtagg gagttatcaa gcccaaggtgcaaattagag gagtcctgag 38041 tctcccagga gcaggcctga ccattgaatc tgcccccttctctgtctttg gctgaaagcg 38101 gctcatggaa gtgaatgggg tgatagattt catagcataggtgccatgct tgttagctgt 38161 tggggaaata cgattaaaaa caaagtctcc tcccaacccagaaagtctct ccacaaatgt 38221 aatagagaaa gaaaatagtt ttcttattaa ataaatattaaaccagaatc tgatatacat 38281 tacaggcaac ctactaagag actgcaaaga cagaaagagatctcacccgt ttttatatag 38341 ccaatatatg catatgaccc aatatataca ttttctcaagttaagtgata actaatcccc 38401 aagagagagg acttggcagc attatttatt aYgcatagtttatcttaaat ttacctggaa 38461 attggggtgg ccatctgtgt tagttaacag actttaaacaaaggacaaat aaacacagat 38521 ctttatgtag gagatagtct tgccatttgg agcctggtggccattgaagt taggctcctg 38581 ctcccagcag aaactgagag ataagggtgc ttatttgtaaaagagatgat taagcttctt 38641 tatttttagt gtgtatttgc ccttctgcag ctaattgtgctccctcaRga cagagatttc 38701 agaggcacat tcctatcaca tgccacaaat gtctaaaatgacagtttatc ttcttactca 38761 cctcgccaat acatattatg tataaagtac ccaaggtttaagtaaataca caaaatctgc 38821 tttctgatac tatatcagga tcgcttagag tctgtggcagttttgaaaca tggctgcaaa 38881 ctctttgatc ctcttccttt tgaggcctag ggtctgcagaccttcctctt aaaactgagt 38941 gggcttgtaa ctgctttaaa caatgacgtg tggtggaaacgacatcatat actttctaag 39001 gctatcaaga aaaactgtat ggcttttccg ttactcactggaaagtttcc tcttacaatc 39061 ctgaaccact tcataagaag tctgacaaag ttgagaccagcatgctgtga ggaagctcaa 39121 gccacacgag gaggccacat gtaggcaata tagtcaaccatttcaaatga gcccgtactt 39181 ctgatcatcc aagtccagtg atagacatga gagtaaaggtctgcttggtt attgcagccc 39241 ctgccacttg agtcatagtc cgctgtctac atattctaaggtgaaggtct agacattttg 39301 aagaagagac aagccttctc atagtgccct gtctgaattactgacccaca gaatctggaa 39361 gcataataaa gtactgttgt tttacaccca ctagggcctattggggtggg ggttggggga 39421 ggaggaacat caggaagaat agctagtaga tgttgggcttaatacctagg tgatgggctg 39481 atctgtgcat cagaccacca tggcacatgt ttaccaatgtaacYgcacat tctgcacatg 39541 tacttcagaa cttaaaagtt gaagaaaaaa tacacaaaataaaataaaat aaaatattgt 39601 tgttttgtat ctctgagttt tggactcagg gtgttttaaagatcaatgca taactaaaat 39661 agaattattt agcacataca taaggaaagt cttttactagcttagttttt attttcttag 39721 catcattatt tatagtagcc aaaagataga aaaaaacccaaatgttcatc aatgaataaa 39781 taaatagaca aaaggtggtt tagtcatgta atcaaatattatttaactat taaagggaat 39841 aaagtactga tacgtacaac atgtatgagt cttgaaattatgctaagtaa aagaaaccaa 39901 tcacaaaaga tcccatatta tataatgcca ttcagtcgaaagtccagaat aggaaaatat 39961 acagatgcag gaagaagatt aatagatgct taggctggggtggggagaaa ggtgggatga 40021 tgggggaata gggaggtgat atctaatgga tatggggtttctttttgaag tgataaaatg 40081 ttctacaatt gacagtggtg atggttgcac atgtctgtggaatatattaa aaatcattta 40141 attgaacact aaatgagtga attgtatatt atgtgaactatatctcaatt aaagcattta 40201 aggcaaaaac aagttacata tgacattttt ccttatagaatatgtaacat ctgtgtatat 40261 ttttcataat tttattatac cttgtagtaa cactattagctcagtcatct ttaattctgg 40321 tgaggtataa ataaaatacc aaaagcttta catcatttcacttttgttga ctattactta 40381 ttgagaaact ttttactctt tggatgagac atgataggataggctatgct gtagcatcta 40441 caatgtaagt ttcatgaaag gttcatttct cggttcacattacatgacta attgaattta 40501 aaaatgcaga gtgaggtgag gatagttttc atgtagtctctcaggaatac aagctagtgg 40561 agtatctaac actaaacatg tgtctttcaa gtccctcacttaggaagaaa agagacacga 40621 agagccttct tttacatatg aatattcaac tgttccaaaatgattagttg aaaagataat 40681 cctaatcttt ttttttcagt ctttaagtgc tcttttcttttattctcagc cccctttatt 40741 gttattatta ttttcatttc attattatta tactttaagttctagggtac atgtgcacaa 40801 cgtgcaggtt tgttacatat gtatacatgt gccatgttggtgtgctgcac ccattaactc 40861 gtcagatttt atgtttcttt atcctggctt gataaaacccattgtctcag gacttttctt 40921 ctaaatagaa aagtagtctg aaggctcgtc ctgattcattttaagactgt agtatttctc 40981 ttctgccagt taatatgttt tcactttttc tattttctttttatatccaa tacatttcag 41041 tatgaaattt tgtgatggtc cactagtctg atgtgcacaatttagccagg aataaatatt 41101 agtggattca aaataggcac tcaaactatc tgtccagattttttaagtgt tgttggtttt 41161 tttctttctt tttttttttt tttcaaattt caactgatgaatgaaagtag ctgcctagag 41221 aataatgagg tgggtgccaa acctgggttc attgaaggcgttatagcacc tgtgccatgc 41281 tataacttag ataagtgacc ttatctaagc ccatggcttcaaatgccact gaaactggtg 41341 aatttatctt ttaggaatgg aagggctata gagatatttgatgaaagaaa actaagagga 41401 tttgtcacca gaacatctac cttttaaaag ggggctaaagaaaattctct agccaagaaa 41461 aataaaaaag aaagtttcaa acaaaaagat taaagaaaacacaatggaaa ggaagaatga 41521 ataaattcat cttccttttc ttcttcagtt tggtaaatgtatttgacagt tgaagttacM 41581 attatgacat tatcagctgt gattgtaaac ttgatttcagtaacatttga agccatgggc 41641 ttagacaagg tcacttagga atagtgtaga tatcaggacaagtaagacct ctgagacagc 41701 catgtgggaa ctcgacatag caagcaagcg atggggagccccacccagtg actgtcttca 41761 ctctagcagc tctcagtgaa ggcttctgta ttaggccattcttgtgttgc cataaagaaa 41821 tagaggggcc ctgcatggtg gctcacacct gtaatcccagcactttgaga ggcagaggcg 41881 ggcggatcac gaagtcagga gattgagacc atcctggccaacacggtgaa accctgtctc 41941 tactaaaaat acaaaaaatt agccgggcgt ggtggcgggtgcctgtagtc ccacctactc 42001 agcaggcaga ggcaggagaa tcacttgaag ccgggaggtggaggttgcag tgagccaaga 42061 tcgcgccact gcactccagc ctgatgacag agcgagactccatctcaaaa aaaataaaaa 42121 aataaaaaat aaaggtaatt aataaaagag atttaattagctcatagttc tgcagggtgt 42181 acaggcatgg cactagtatc tgattagctt ctgatgaggcctctggaagc ttacaatcat 42241 ggcaagaggc aaagggggag gaggcgtgtc acatggtgagagtgggagca agtagaggag 42301 gcgccacaca cattcaaaca accagatctt gcatgaactcagagcaagaa ctcactaatc 42361 accgagagaa tggtgttaaa ccaggagtgt ccaatcttttggcttcccta ggccacgccg 42421 gaagaaaaag aattgtcttg ggccatacaa aaaaatatactaacgatggg tgatgagcta 42481 aaacaaattg ccaaaaaact caatgtttta agaaagtttatgaatttgta Ytgggtcata 42541 ttcaaagcca ttctgggctg catgcagaat gctggccatgtgttggacaa gcttctgcta 42601 caccattcat gaggaatctg ccccatgatc caatcacctcctaccaggtg ctatcacaac 42661 atcgRgaatc acatttcaac atgaggtttt caggggacaaacatccaaac catatcagct 42721 tccctgtgcc aagcatggat ggagctgaga gctttatatgggttacttac aaaatataca 42781 tgttttatac aataatatta atagtacaaa ataattactttgtaccaaaa attaattgta 42841 caaaatatta aaatatttaa cataacattt gtacaatgtatataaaaata taaatatccc 42901 ataaaatttg ataatagcat atgctatttt atttctataatatagacact cttcaatact 42961 cagcaacttt gttcacatta gaccactttt atttgtaattattgatatgt tgggtcacaa 43021 acctgccatt ttactttRtg atttctgctt atccgtttttttttcttttt ttgtaattgt 43081 tgttttgtac gccttgcttt catgtgtgtt actttaattttcttttttta gaattccatt 43141 gaggtttatc tgtggtattt ttgagtatat ctctgtgtgcagacatctta gtggatgctg 43201 taggttttac agcacatgta ataacttatc atggtttaatagggtcaaaa ttttagtact 43261 ttgaaagatg tacggaaacc ttattccttg attccctatatgtctcttta ccctcctctg 43321 ttccttatat agttgtctta aatatttacc tatatacacttaaaatcgca tctgataatg 43381 tcataatttt aacttcagct gtcaaataaa tttaccaaactgaagaggaa aaggaagatg 43441 aagttattca ttttttccct ttccattgtg ttttctttaattttggtgtt ttaaagtttc 43501 ttttttaatc ttttttcttt ttttttttcc tggatagagaattttcttga gccccttttt 43561 aaaaggtaga ccttctggtg acaaatcctc tcagctttctttcatcaaat gtctttatag 43621 cccttccatt cctaaaagac aaatatacca gatataaaaaccagaattga aagttatttt 43681 atttcatcac atagacagtg ttgtgccact tttctatggctttcatctat ttctgatgtt 43741 tcctgcttcc atttgtattg ttttctctcc tatagatgagctatttcctc tagctgcttt 43801 caaaatttgt tctttctttt aattttcaga tattttaccatggtatacct tagtttggat 43861 tctttgtgtt tgtcttggtt agttttatgc actgaaagtttgtgtcccct caaaattcat 43921 atgtagacat cctaaattca atgtgatgtt atttggaggttaggcctttg ggaggtaatt 43981 agggttagat taagtcatga gggtggggcc ctcatgttgagatcagaggc cttataaaaa 44041 aaaaaaagag agagagagag ggagaaggag acaagagatatctttttctt ttctccaaca 44101 tgtgaggata caatgagatg atgtccatct atagccaaaaaatgtcttca gcagaactca 44161 tctgtgctgg cattctgatc acRgtcttcc agcctccagtattgtgagca acacattttt 44221 gtttttataa accacccatt ctatggtatt ctgatacagtagcctaaaat ggctaagaga 44281 ttttgggttt gcttgaattt aggaatctgt gggtttttgcctattgccaa attaaggaag 44341 ttttcagtct ttgagacctt tctcaaagtt gcccatttcttctctccttc tgggactctg 44401 ataacacaaa aattaggcct tcagttataa tactacaggtccctgaggct gtgtttatag 44461 ttttttcctg cttaccttca tgaatttttt atgtggttattgtaagtttc agttctaaaa 44521 tgtccattca gttctttcat atattttaat tttttggctgagataatttt taaatttgtt 44581 tcatatatgt ttgtaagcaa tatactggaa gcatttttatgatgactgct ttaaaatatt 44641 ttctgaataa ttccaacatc tttgtcattt ttgttctggaatcttctaat taccttttac 44701 attcaagttg agatttttcc tggttctttg tataccaaatgatttctaaa tgaaccagga 44761 cattttaggc attatgagac tctagaactt atttaccttttctgtcttag ctagcttcct 44821 cagacactac tccagcaagg caagggagtg agggagggacactgcattac tgccaggtga 44881 tgatagaaat aaaggtttcc tctcaatctc tgttgacacctaggcaggcg gaggttcctc 44941 attactgctg gctggagggt ggattctggg tcaccactgggcctccactg atagcctgtc 45001 tcggaggaag aagtgacttg ttactgtttc ccacatggcctccacaagca ccacctggta 45061 tggaaaggag ggtgcttctt cattgctgct tttagagtagatccagctcc ccatttgctc 45121 tccagtcata tcaaggtgtg gaaggagagt gaggggcttattaccacaag ttggggatga 45181 aagttccaac ttctgctatt ctgagatact attctggaggagggttggga caccttgtca 45241 cagcctggct ggtatgaaga tctaaagatc taagctcttcacttgaggta atagtgtttt 45301 ctgtggtact tgactacagt agagtggtta ttgtcgaaatgttttctgcc ttggtagact 45361 atttccttga cctttgccta aaataatagg cttttgttgggaaattctca tatgtgcctg 45421 ttagtatttc tatgttgatg gcattttcag ctaaaagtctgcaaattata aagcagaaaa 45481 gaaaacttag aggattcatc actatacaat ttattaagtccctaccacat ctgccttggt 45541 ctctccaggt ctgagttttc atatatttta tatataatgttcagggttta tagtttttct 45601 tagtagaagg aatagggcaa aatacattta ctccatttttctggaagcag aaattggttt 45661 actttctgta agaaagcagt tggtgaattt ctgtctaaattctctgcctt ttcaagagga 45721 actgggcttt acttctcaga taacaactga gcaacgatagacctacaggt ggcattactc 45781 tctgtatagg tagagaatag gctgaatatt aaatgttaaggagcagaatc attggaattt 45841 aaatgaggaa ggagacttag tttttattca cgttaaccagccacctgatg acatgcaaca 45901 gtgcgtttgt tcatcaacat accttttaga tggcctttctgcttcttttt tgaatggctt 45961 taatgctaaa gaaagcaagt gtttatcaaa taatctattttttagataat tcattattag 46021 aagttaattc attattagaa agataattta ttattagaaggtaattcatt attagaaaga 46081 acaaaacgag tcttttttaa taaaagaata aataaatttaaatgtattga acttttattg 46141 ttgtatgtca tgtagggtaa ttttgtatac atgttgcattttacaagact taaatcgtgt 46201 aattttatat acatcttgta gggtaatttt atatacattttataaggtaa ttttatatat 46261 attatgcatt Waattcttgt aagtagccct ctgccttagccagttcgagc tgctattaac 46321 aaaatatctt atcttgggta atttataaat aatataaatggatttgtcat agctctggag 46381 gctggaaagt tgaagattga gatggtacca gatttggggttgagtgagag cccattccaa 46441 acagacaatt tctcctggct tctctctcat gaggcagaagtatgaaagaa acatgaacat 46501 tgagtcccta catgtcaaaa gagatggaac agctcagcagcctttgggag cttctttttg 46561 tagggaaatt gatcccattc atgaaagcag agtccacacgacttagtcac ttcccccaaa 46621 gactccatct cttaatacta ttgcataggg aattaagtttcaacataaat ttattttgta 46681 gagacataaa cattcaaact atagcattct gtccctggtcatcccccgaa atatatggtc 46741 ttctcacata ctaagtacat ttatttcatc tcccaaatcccaaagtctta actcattcca 46801 gcatccactt taaaatctaa atccaaagtc ttatctaaatatcatcaaaa tcagatatgg 46861 tgtggccaat tttctaatat tgaaccatct ttaacatcctcatcctcttt caacgtggtt 46921 tattattctt ttagcatact gttcgtttgt ttatgtactattgaatagtt tccctaacag 46981 agtatccttg ctgtcctata tattttgttt aatgaaactcagaatgaatt cagaaacttg 47041 ttgtttcctg tacttaaaaa atctaaaaaa gaattatgtgctttttaatg ataaagatta 47101 gatattaaaa ttttctgtat tcagcgacat tggaggtgataatgagaggt agctggagac 47161 agttacatat caaattcttt tgcttcttag ggttattttctattcaactt tgcctcagca 47221 acttgagtta aatttggtag tctatattat ctgacaataatactgatatt gctggaattt 47281 ttacaatcag aacagagttt tactcaaatt tcttgccctttaaagtacat tatgctgcac 47341 cctttatgaa atccactcaa gtttttaaaa agaatggtgaacgacttagg gaggaagaga 47401 gctgagcaat gaaaaaagag gagaaaagag catagtgagttttatagaga agagatctag 47461 aactacattt atgcccctag aaaccaggat taatagttttggaaatttta agaattcccc 47521 agcacttgca actccctctc atttgttata tattccttgttttcctagag ttacaagaat 47581 atcaatgaaa gatgtaaaaa taattttaag aatttgcatttgtgctatgc aatctaggac 47641 agaaggggaa caatggaact ctttctcctt ggcagctggctttattaaga taaaggagga 47701 gggaagcctg ctggaagcat caatgagcaa gagtcccagatattcttaga ttgtattaat 47761 ggccctcaaa ttctagactc agaaacaccc acaaagagaagcaacaatca gaaattgcat 47821 gctattaata ttttattggt atactgaaga aagagttatgaccacattat ttcaatgtca 47881 tggctttctg aaggaagtta tcttcttatt tattttctgaaacaaaaaaa aaaaccaagg 47941 aaatttatac acaacataca acatggcaaa acttatctaattccttgatg atattactga 48001 agtaaattat tgctttcaag attaagaaaa cagatttttttatggtgttt atgttttggt 48061 tactgttcaa aacattcaag gttatattta tgaaaatgactgcaacttga acctgtagta 48121 gtgaaaaact aatacaagcc tacatatcaa ttaacagtggttgactgagg aaatcatggt 48181 acagtcacac aagagaatag tatacaattg taacaacaaaagaaaaatgt acaaataaat 48241 caccaagaag atctataggt ctccgtgaaa tgataaagttgtctaagaca tgtattattt 48301 gaaataagca aatacagatc tgcatataaa ataatatgtcatttgtggga aaaataatga 48361 cgtatgctta tatacatata aatatttcca ggaggatgttcaaaaaatta agggtggtga 48421 cttttggtga agggtgtggg taagtaggat aaggcgtcactttaagattt agcttcatac 48481 cattctgttc agttagattt ttttttcctt gagcatgtactaatttcaaa ataagtattt 48541 taaaaattaa ataagatata agagcatcaa gttcaagtatcatttagcag ggacactgga 48601 cctgagaggc tagtgtgagg caggactgag caaagagtcctctggcggaa acctgagaga 48661 aacctgagat ggagagtcct tgggtttgag aaggcagactcaaagacagg aggcagagca 48721 ccaaagggag agcacacccc acttcctcat taccaactcattttaatgag acaagtcatc 48781 ttaagaaaaa agttggggag gtcagtattt caaaagataagaattctttg aaccacttgt 48841 taaaaataca aggacttgca accttatcca agaatatctgaatacaaatc tttagaaatg 48901 gggtccagga ttatgtaatt tcaacatatt tccaattgatttgtgaacac gataaagttt 48961 gggaatggta gcagtttggg catttaatgg agaatgaactggaatcatac ctgccactga 49021 attctagatt accagagtgg ttgctcctgg ggatgtcttgctggtctgtc cctcYggaag 49081 aatgatgatg cttcctgcag gctgacagaa ggaaatcggggtagagagag ttgacggtgt 49141 cctcgtYggg gtggtcgttg ctgattttga aaaggaggtSggggaggatg gcggtgatgg 49201 cctcctggtt ttggtggtct ctgctgagga ccatcatcttggttaccact atcaccaggg 49261 ggtctaggta ggagtccttc aggaggaggt ctctggggtccctgatctgg tctctgactt 49321 gagtcctcta catctgtgtg agtaattaat ggacaagaatgcatgagctc agtgaagaaa 49381 aactctcctc tctttatact tctctcacct taccacacagccctgttctc cccaaacctg 49441 ctgcttgact gtcaacttct tttgtgccca ctgttttttttttcttttta tgcagctctc 49501 aacttaatcc atgtaagagt gatatggaaa gtctcatttatttgctcctg tgacaagtat 49561 gtttccttaa atgaaaagag gggttcttgc atatcttagagttgccagag gatgtctcca 49621 aggacagaaa aaaaaaaaaa agcaaaagca ttatagctttcagttatgtt actggttatt 49681 ttttattttt attttttggt tgctgtaatg acttacaaactgtgactcca tctctgggca 49741 cctaagccct atgtggaaac tagagctgat ttcccaggctcctggaacca agtagagaaa 49801 tactaagaga cagaagagaa agacaatgtg aaagcctgcacatgatcact aaacagctgt 49861 ggcaactatt tatcatctgt agtgacacag ctgagtgggtcaacacagag aaaagacatt 49921 catgcagtga tttttttgtt ttgttttgtg ttactccctgaagccacata accgtgcggg 49981 ggaacactgg gggaagaaag aagcagttaa ggatggaggagtcagaatga gaacacttct 50041 catttgttca tcatctctca gcagtcattc cagcctgggaaaaaagcctg cccactccta 50101 ttgtcctcaa agccaggcat ctctgtattc aagttggtggcctggctcat ggtccccaga 50161 atcaaagatg ggtaagaatt gcctatatat ttaggggcactaatattaat caatgcctga 50221 cccctcttac cacctcctcc caaaaaaatg atgagaagaagacactgaag gaaactaaaa 50281 agaaattcta gtggaaaaag Raagaaaaga attggattgaggatcattgg gatttacctg 50341 gtatggtgaa agtaaagtct tcatagttca catctaggaaaagaagcaca ggatgaagtg 50401 aacattacct cataaatctt tcagggctca tagtggtctatagggaaagg ggtcttctgg 50461 ccacaccctg tgcatcccct aagttacctc atcaaccatgttctgtgaag ctgctggaag 50521 gggaggaaga tgttagggga ggcaggattg ttactatcgctgagcatcaa ccacgaattc 50581 aatagaagag tccctttttg tcatccttag gatccctcaaaccctaatgc taatttaggc 50641 acacttctct gggtattttc atgtcatatt ttagatctttaccttcttcc ttctttaaat 50701 attttgatca tattcacaag cttgcccaag aggagcaaccaggagtaaat atttggatat 50761 aatcttacac atRctgcctg caacatattg aaattttaaatgtgattgga agaaaaatgg 50821 gaatttgggg acctgatatt tctataccac tatgtagaaagacacaaatg tattatccag 50881 tcctctatgt ctgacagtac tctgatgttt gtttatctctttcattgatt ctgcatcccc 50941 tgtacagcaa ggacaccatc atcgctgtcc actgagattactgtagcatc ttcatagctg 51001 gacttccctg gatcagtctt gcattctcca ctgcatccttcacagcacag ccaggtcttt 51061 ctaaaaatac aaatcttact ttgtcattcc tctaattgaaattctctatt gggttccaag 51121 tgtatatcaa ataaagtata aggccttggc atggaatgagggcacaacag atgtcatcct 51181 tggaatgaga gctccaaagc cctacccttc ttctcaacctccttcctccc ctcctgctct 51241 tcYgctgcag tggtgtaggg tgaacccctg aggccctagaagagtcatgg cccctcatcc 51301 cactccccat ctcctttttt aaaaaaataa tttttttaccattatctgtg ctctgagctg 51361 agctcagagc cagaaggacc actgagagca ggaccagcagcatcttgaag gaggctctgg 51421 agttgctccc aactctgcgt tgagagaaac atggcagctccctttataaa catgagcaga 51481 acaatggcac ctttgagctc catactgggt ggacctcaccacctgaaagR ttaggttttg 51541 ctttcctctc ataaagttga atgcgtctct tattactttttgggatttta gcccagcagg 51601 atgtggtggt aggatgtggt taggtacaaa ctgtgacttgagcatagttt ctgaagaaac 51661 agtatccaag caatcagcac aaatactaca attgaagtttggtcatcatt tgattttcaa 51721 ttgggttaga cagttagtct ttccccttca ttggttgtctgtgtgcacac gtgcttacgt 51781 ttgtatatgt ttgtcagcag tgatcttata gccagaagtgaaaatgatta atatctctag 51841 ctattagtgt agcataaatt ccatttttat agcatatatacctatatgga tgatatgtag 51901 cttaatttca taataatata gagatcattt ctgtttaatgttgagttgtg tgaggacaac 51961 acacacttgg gcacacgtgg atgacagaag ttttaccctcaggctactct ggcccaaaga 52021 gattaagatt ctcctaatct agcattaacc agagatactttattgttgta gaacacaggt 52081 attaaaacat tctatcttta ttaataYcct ggaggttagtaaggcaaaat tggccacaga 52141 aagcaccatt agatttctgc atcagaacag tgtgaatacacagttccttg aacaagccat 52201 gctattttat gtccctaaga cttcttccat gatctttctcttttctagaa tgttctatat 52261 gattcagttt aggtcttact tcttgtacca aatattttaatcacactggt tattgagtgg 52321 acacaataat acaatctaat tagatagtat gatagcctgttgaaagcctc aagtattaca 52381 ttgattttct attttttaat tagtttccat tctatttgcttctcagattg cctttaccaa 52441 agattatttt ctctgcaata ttcttatact cattcttcttcactcttgtg ctgaacctta 52501 cttgttgata tgttatattg tgaatcaatc ctccctatgtctatgtttta tatcttactg 52561 ctttcttcat gcgttctggg agaaatcttt atttgattttctaaacaagg ttctgtactt 52621 ctctttgctt gctttcttct tttttgtttt taaaatttggcaccaaggtt ttgtgtttct 52681 gaaaagtttg gtagggtcct gagttggtag cttaatcttcacctgctgat ttttctgtcc 52741 tatctagtag ttttctgctt atattttcat ggaacccctagatcaatcag tatcaccagc 52801 tgccactttt cattacaggc Rttgtatagg tctcgtattattgttacatt tttgtacagg 52861 gctggtgata atttgaggat agcttggttg gaagtatgacagggtcgggg gaatgtgact 52921 ggaaatctta aagacaggcc tcttgaccat tgtggataagtggatcctgt gctgtgcaga 52981 tgaaggtctg gtctggcaat tctgaagggt tggaggtaatattacaggaa gattttctaa 53041 tcaatggtgt ttgttatgga taactttact ttggcaaagtgaccaaaaaa taaatatttt 53101 ctctggcata attatggaca tattttgaag gtgaaatatattatgagtta gcaattcttc 53161 tctatgtggg aagcagaaga gttctagctg tgctttagggttttatgggt gagagagagt 53221 agttgtttgg gcctacagac ccagctgtgt tactaggattgctgcctctg atttcaccag 53281 acactgttta acatgggaag tggagataag tagttctgcctaggtggctt atggtagacc 53341 Rtcaggtagg aaaggaaggt cagcgagaag gagcaSaggggcgtgccatg cagacatgat 53401 tcactgtgtg ggtggctgag gaaactgcag tcctggtggaaggcaggctt ctccttggct 53461 ggtgagtgca ggaagacaac aggtgatgcc tgcaaatatcactcagctca gacccttacc 53521 ctgacattct ccagtgtaag caatgagaga aagagattaatgtatcagga aataataaac 53581 atcagctatg atctcaccac acacaaccac aacagaggtgacggaggagc tgaaacagac 53641 actgtcagtg agtgagggtg gggtcaaatc gagaagcaggactgcagcag gagcttaggc 53701 acttgtaggt gagtggacac tctcagcaat ccacttgccttttacaacag atgaaaactt 53761 aacatacccc taatataatt cttgatttct acttcctaaaacctgctctc cttcatacta 53821 atttgttatg gctaccctaa aaaaatgccc cacaagtggcttaaacaaca gaaatttact 53881 ttctcatagt tctggagctt gaagtacaag attaaggtgtcaacaggttt ggtgtcttct 53941 gaggccctgt ctccttcact tctagatggc cggttcttgctgtatcttca catggggttt 54001 tatctgtaga cgtgcatccc tggtacccct ctgtggtctgtgctaaYgtt cttttcttat 54061 gaggacaggt gtcagatggg attagcacca accctatcagcttcatttta acctaatcac 54121 ctctttaaag gccatgctgc aaatagagtc acattcagtggtatcggggg taagggattc 54181 aacatgagag tttggaggga acacaattta gctgataacacctcacaatc ttttctcagt 54241 tcatgacatt tgtttcactc aaccattcaa aaacataagtgtcactcttg attgtttagt 54301 ctcctccgtg tactgccctt agcaaatcca attggttctaccttcaaaag atatcccata 54361 tatgatcagt ttcataatct ctatcagaag gYcctgagtcaagccatgat tctccctcct 54421 ggaggacttt caacagtctc cagctgatct tggtgacattcctgcccctc accacatccc 54481 aWgcagttct ccttacaaca gccagagtga tcttttaaaaatagaattta gaacatcagc 54541 aagatggctg actagagttc cctggcactc atttccccacaaaaagaaat caagaaaaca 54601 aacaatttta tgtcagccag tgtgatttaa aaagtatgctggagaccacc aggggaacag 54661 aaacacctct gtgaagtaca gaaactcaat atagcatcctgcctctgaca tactctctct 54721 tcagtcaagg ttggctcagg gtcatcagta actccattctacaggaaaaa gtaagctgta 54781 gttccccagt ggtccacatt gccataaaaa atgtcaacaatctttgcccc actgttctca 54841 ctggcacaaa aaccagtctg gagagcagtt gggatttccaaactgcattg ccttacatag 54901 taagagacca ccttgagcac cctctgcccc tgcaatgtaaggtgctatgg ctcagtgcca 54961 tctccaaact gaacctacat agagagtgtg tcttgctttgagcatcagta gcacctggct 55021 ctctatcact tgaggcccca ccatcattcc aacacattcatacaggtatc tgcagcacca 55081 tgaccctagc ttcccagagc ctagccagat aaactaatcaagaccccggc atccgaaccc 55141 atgtggtgca ccaccaatcc agggaagaag tgaacagcagggaagccacc catctgccta 55201 ctagccacga cacccacatg cacccacacc tcacaaccagctagtctgct gagcctgtgc 55261 atgctggtgc ccagcctgaa aactggtcct gtggtgggcccacccccagg actacagcct 55321 gcttgggcca caacaactct gagtttgtgc atggtctaataattggtcct gcagtgaccc 55381 tgcctcctca gatgggactc tgcagagatg gatggccctgctacacctgt gagcactgac 55441 agcaacccgc ccacccagca ggaagacMgc agtgcatgtaagtgcacaca ccttgagaaa 55501 aggctcttcc cactgctgct ggtggcacag ttgctgctgtcaccaccggg ggctgcagca 55561 gtgaaatgcc agttggaccc agcaaagtgg caggatcctcagcattctag catatgcagt 55621 gttctgcacc tcaggcactg gaaaggctgt gaaccagacatagggagcca aagcacatgc 55681 tttccagaac cagagagctg cctccctgtg gctgctgacacagacagcaa tgtcaccccc 55741 gcaacacagc agcagagatg ctgcacactt gcatgcaacctggggacagg ccctctccat 55801 ctgctgctga gtctgctgat gcagctgggg gccagagcatgtgccactgg cagtgacctg 55861 acttccacca gcagcagagc cactgtgaac ttgcacgtaccctgaggact ggcttatgct 55921 gctgccagaa gccaaagtgt gctccacatt gccagagagctgcctgctag tggctgctgc 55981 cactgacaat aaccacaccc tgccctagca gcacagctgcagcaaatttg catgtgcctc 56041 gaggacaggc tttcttcagg tattgctcct gctattgggagatccaagga ttaccccatc 56101 caacttacca cagccaacat ccatgtacat aactagggggactgaggaca ggcctgccaa 56161 gcctggttcc atcatcccag tgcccaggca cactacccaggggtgtggca atcatcctgc 56221 tttgtccacc accactggca tttgcacatt cctttaggaggactgagaat gggcccaacc 56281 agcctgacag tagcactgta gccagaaaca atatgcatgcaacatctgaa ggtctggaga 56341 ctggccccca gcccatcaca accacctcta acaccagagcttgcaacttg agaatctgag 56401 ggttgtccaa ccaagctaca gaaattgccc atggcaagcacattcccaga ggcctaaagg 56461 cccacccacc ctttcagcct accattgcaa ctgctagtacccaggtaagc tacttaaagc 56521 ccccagaatt gttctgctta aagctgctaa aactggtacaaacatatgtt gcctaggtgc 56581 caaatatagg cactctcagc ccactgttac ccctactggggcccaagaac tggcccacct 56641 aaaatctctg tccccaggaa aacctcacta cagcctccaatcacaactac agcctaagcc 56701 actgaggaag tcacagacac cacttatgct gttaatagctgaaggaataa tataagacca 56761 ccttactgta cacacacaga atcaaagcca aagtgtcctactgaatcaac accacagata 56821 catcttcaga aagaaaagtt aatgttttgg atctatgtcactgcccaaat ctcatgttga 56881 attataatct ccaatgttgg gggaggggct tcattggaggtaattggatc atgggggtgg 56941 atccttcatg aatggtttag caccatcccc tgcgtactattcttatgacR atgagtgagt 57001 tctcatgaga tttgcttgtt taaaagtgtg tgtcacctcccccccaccca gtccttctcc 57061 tgctatgtaa gatgcctgct cctgctttgc catttgccatgagcaaaagc tccctgaggc 57121 ctccccagaa gcagatgtca ctatgcttcc tgtacaacctatgaaactgt gagccaatta 57181 aacctctttt cttcataaat tacccactct caggtacttctttatagcaa tgtgagaatg 57241 aactgataca gaaaattggt accgaagagt agggcattgctataaagata ctaaaaatgt 57301 ggaagcgatt ttggagatgg gtaatgagca gaggttagaagagtttggag gactcagaag 57361 acaggaagat aagggaaaat ttggaacttc ctaaaaacttgtaaattgtg accaaaatgc 57421 taatactgat atggaaaatg aagtctgggc tgaggagatctcagataaaa ataaggaact 57481 tattgggaac aactggagca gtcacttttg ttatgctttagcacagagcc tgggtgcatt 57541 gttcccctcc tttagggact tgtggaactt tgaacttgtgagtgatgatt tagggtatct 57601 ggtggaagaa atttctaagc agcaaagtgt tcaaaatatgacctggctgc ttctaacaac 57661 ctaagctcat atgcctaagc aaagaaatga gctaaaactcaaatttgtat tWaaaaggga 57721 agcagagtgt aaaagtttgg aaaacttgca gcctggccatgtggtagaaa aaaaattccc 57781 attttctaga gaagaattta ccctagctgc agaaatttgcatgagtaaag aataaccaaa 57841 tgttaacagc caagacaatg ggagaaagac attgaaggcatttcagagac tttcgtagcc 57901 acacccacat cacaggcctg gagtcctagg agggcagaatggtttcctgg tccaggcgca 57961 gggccccact gacctgcaca gcctccaaac actgtttcctgcatcccagc tgctccagct 58021 ccagctgtgg ctcaaagggg tccagctgca acttgggctgctgcttcaga ggatgccagc 58081 cataagcttt ggcagcttcc atgtggtgtt aagtctgtgagtgtgcagag tgcaagagtt 58141 gaggtttggg agcctctctt ttgatttcag aggatctacagaaaagccta aatgtccagg 58201 cagaagcctg ctgcagaggc agtgccctca cagagaacctctactagggc agtgcaaagg 56261 gaaaatgtgg agttggaacc cctacacaga gtccccactggggcactgcc tagtggagct 58321 gtgagaagag agctgtcatc atgcagaccc cagaatgacagatcctctgg cagcttgcac 58381 catgcacctg gaaaagctgc aggcactcaa tgtcagcccttgagagaagc tgtgggaaat 58441 gaaccctgca aagctacagg ggtggaactg aaaaggccttgggaacccac cccttccatc 58501 agtatgcctg gttgtgaaac atagaaagtc aaatgaggttattttgcagc tttaagattt 58561 aatgacttcc ctgctggttt cagatttgca tggggtctgtagcccctttc tttttacagc 58621 tttctctttt ttggaacagg agtgtttacc caatgcttgtactccccttg tatcttggaa 58681 gtaactgttt tttgaattta caggctcata ggtgaaagagactagccttg tctgagatca 58741 gcctttggac tttcgacttt tgagctaatg ctgaaatgatttaagacttt gggggactct 58801 tgggaaggca tgattgtatt ttgaaatgtg agaaggacatgagatttggg agtagtgatg 58861 ggctgaatga catggtttgg atctgtgtcc ccacccaaatctcatgttga attgtaatcc 58921 ccagtgttgg ggaagactgg catgttggga ggtgactggatcatgagggc agattcttca 58981 taactggttt agtactattc tctcactgct gttctcatgatggtgagtaa gttctcatga 59041 gatcttgttg tttaaaagtg tgttgcatct cgcccccacttggtcctgat cctgccatgt 59101 aagactcctg ctccagcttt gccttttgcc acaagtaaaagctccttaag gtttccccag 59161 aagcatatgc ttctatgctt cctgtacagc ctgcggaaccatgagccaat taaacctttt 59221 tttgaaaaat aaattactca ctctcaggta tttctttatagcaatgtgag aatggactga 59281 tacaaaaatc ttcccctatg aaagccaatt taaaaaacagaagaagcaac tatttcacca 59341 gatgcgcaga catcaatgta aggacacagg aaacatgaaaaagcaagaaa atatgacacc 59401 tccaaatcaa cacaatactc ctccagcagt agattccaatgaaaaagaaa tttatgtaat 59461 gttggggaaa atatttaaaa aatgatacta atggagctcagtgagataga agagaacaca 59521 ggtaaaaaat agaaagaaac cagaaaacca tttcaggatattaaagagaa attcaacaat 59581 gaaatggata tcataaaaaa gaatcaaaca gaaacgctggagataaataa atcaataaat 59641 gaaagaaaaa tacatctgaa agcttcRaaa atagaataggtcaRtgagaa gaaagaatat 59701 cagaacttga aaacaaatat tttaaataac ccagtcagacaaaaaaagag ggataaaatg 59761 tgaaaaagat gaacaaatcc tacatgacat atgggatgccataaagcaac caaatttttg 59821 aatttgggtt tttcccaaag gcaaaatgaa aggcaaaaacataaaaaacc tatataatga 59881 aataatagaa gaaaacctct caagtctagc aagagagttaaacacctaga tacaggaagc 59941 ttagatagat acaacccaaa aatattttct tcatgtcacattatactcaa actgtcaaaa 60001 gtcaaagaga aaaacactat agaagtggca gagcaatatggtggaataga aggctccacc 60061 aatcatccca cccacaggaa caccaaattt aacaactgcctacataatca aagcacttcc 60121 ataagaacca aaaatcaggt gatcactcaa agtacctgcttttaacttca tattactgaa 60181 agaggtactg aagagggtag gaaagacagt cttgaattgccatcgccacc cttgtcttaa 60241 cccagcagca actacataga atggagaatc tgtagacttggggagggaaa gtgtggtgat 60301 tttgaaactt cgcattgaaa tcagtgctgc cttgtgacaatggaaggcaa aaccggggtg 60361 aactcagctg atgtccaccc acagagggag catatagaccagccctagcc agaagagaac 60421 tgtacattcc agcagccaga acttgagttc tggcaagccttgtcactgta ggctaaagta 60481 gtctgggtct ctaaataaat ttgaaaggca gtctaggccacaaggactac aaatcctagg 60541 tgagtcctag tgctcagctg ggctcagggc aagtagactggtgggggcgg tgggtacatg 60601 acctactgaa acatcagctg gggtggctaa agcagtacttgggccatccc tccctcaacc 60661 cccagctaca cagcttatgg ctccaaaaga gaccacttccttccacatga ggagaggaga 60721 aggaagagtg aggaggactt tgtctggcat cttggataccagcttagcct cagtaggata 60781 gagctctggt cagagtcatg aagccctgat ttcagactctagctcccaga tgacatttct 60841 agacacacac tgggctggaa ggaaacctac tgcattgaaaggaagagcca agtaatgaaa 60901 gaaccaatca cctgctgatt aaagagcact tgggccttgaataacaagcg gtgatatcca 60961 ggtagtatgc tgtgggcctt gagtgagact ctgagatgtgctggtttcca gtgagaccca 61021 acacattcct agctgtggtg ggtatggtca gagattcctcctgcctgaga aaagcagaga 61081 gaaaaagtaa aggggacttt gtcttgaacc ttaggtaccaggtcagccac agagaagtat 61141 aacaccaagt gggctcttga ggtccccaat tccaggccctggctcttgga cagcatttat 61201 ggacctgctc tggtccagac ggggagccca ccactataaaaggtgagtcc tagatctggc 61261 agcattattc accacaggct gaatgaagag cccttggtccttaagtgagc atcagtggta 61321 gcctgagaat attcccatag gcttgtggtg atggtggccagggggcaagg tgcctgcata 61381 tgaaaaggga agattagaat gggaagaatt gcatctactggtttgagtgc cagcttaaca 61441 gcagtacaat agaacacaag gtaaatttcc aaagttttggactctggtcc gtggctcctg 61501 gacagcattt ttggacctgc ctggagctaa gggatcttgctgctatgaag agaagcacat 61561 aagcctatcc gtaagcccca aagcagtact aagaaggaagattattgcta tatgtgccta 61621 catcaaaaaa taaaaaaact tcacataaac aatctaatgatgcatcttaa agaactagaa 61681 aagcaagagc aaaccaaacc cgaaattagt agaaaaaagaaataaagact agagcataaa 61741 taaatgaaat taaaattaaa aaatacaaca gaagaatgaaacaaaaagtt tgttttttga 61801 gaagatgaac aaaattgaca aaccttttga cagactaaccaggaaaacaa gagagaaaac 61861 ccaaataaat aaaattagag atgaaaaagg agatattacaacctacacca cagaaattca 61921 ggggattatt agagactact gtgagcaact atatgctaataaattgaaga aactgataaa 61981 ttcctagaca aatgtaacct gccaagattg aactatacagaaatccaaaa acctgaatag 62041 acMaataaca agtgatgaga tcaaagccat aataaaaactgtcctagcaa acaaaagacc 62101 aggacctgat gacttcaatg ctaaatttta ccaaatatctaaagaagaac taatactgat 62161 ccttctcaaa ctattctgaa aaatagaaag ggaaggaatactttcaaact catttggtga 62221 ggtgaatatt accctaatat gaaaactaga caaagacacattaaaaaaaa tacaggccaa 62281 tattttggat gaacattgat acaaaaattc tcaacaaaatgctagcaaac agaatgcaac 62341 agcccattaa aaatacaatt catcattact atgtggcatttatcccatgg atgcaagaat 62401 aattcaacat gtgcaaacca atgaatgttg ataaatcatataaacagaag gaaggacaaa 62461 actaatatgc attgcaatag ataatgaaaa tcatttgataaaattcaaca ttccatcatg 62521 ataaaaatca tcaaaaactg ggcatagaag gaaaatgcatcaacattatt aaaaccatat 62581 atgacagacc cacacctaat atcatactga atggggaaaaactgaaagct ttcctctaag 62641 atctggaaca tgacaaggat gctcactgtc atcattgttattcagcatag tactggaagt 62701 tctaactaca gcaatcagat gaaagaaaag agaacggaagggaagggaag ggaagggaag 62761 ggaaagggag ggaaggggag ggaaggggag gggagggcaggggagggcag gggaggggag 62821 gggaggggag gggaggggag gggagggaaa agaaaagagagcatccaaaa tggaaaggaa 62881 ggagtcaaat tatctttgtt tgcagatgat qaaactttatgtttggaaaa aaataaagac 62941 tccacaaaaa tgtattagaa ctgatagaaa cattcagtaaaatttcagga tacaaaatca 63001 acatacaaaa ttcagtaaca tttttatatg cccacagggaacagtctgaa aaagatcaag 63061 aaagtattct tatttacaat agctacaagt aagataaaatacctaggaat taaccaaaga 63121 agtgaaagat gaaaagtata aaagtataaa acattggtgcaagaaattga agataacaca 63181 ctaaaatgga aagacattct atgttcacgg attggaagaatcagttttgt taaaatgtcc 63241 atactaccca aagcactctg cagattcaat gcaatccctatcaaagtatc aaYgacattc 63301 ttcacagaaa cagaaaatac aattctaaaa ttcatatggtaccacaaaag actcagaata 63361 gccaaagctg tcctaagcaa aaagaacaaa tttggaggaatcacattacc tgactttaaa 63421 ttgtcttaaa tactacagaa ctatggtaac caaaatggcatgacactggc atagaaacag 63481 acatKtttgg actgtgagcc tggcctctat gaaccatcgtttcagctcct gattggtcca 63541 gagccaaggc cctgggccaa gctgagtcac aagttctccaagacagccca tggactaagt 63601 gcattccttc cccttcccag tccataaaca ccctggaccccagcctcata gagggcaatc 63661 catttgggtc cctctttccg ctggcagaga gctttcttcttttgtttgtt aaacttatgc 63721 tttaacctca cattgtgtct gtgcttctca atcatcttggacgtaggaca aagaactYca 63781 gatattatct cagacaataa gagactgcta tctgggtccgttggcaaaac tacaacatta 63841 caacaagaaa actttgggga aactctccag aacaccatagtgggcaaaga tttcttgagt 63901 aatacctcac aagcactggc aactaaagca aaattaaaattaaaaaaaaa cttcaaagat 63961 aaaaattatt aaaaagagaa aagcatccag tcatatgtaagggaactatc accagatcaa 64021 aagagaattt atcaacagaa agtttatagg ctaggagagaatagtatgga atattcaaag 64081 ttctggaaga aacactttca gtcatacata ctatactcagaaatgttatc cttcataaat 64141 aaagaggaaa taaaatcttt cacagataag caaaagctttagactaacct tacaacaagt 64201 gctcaaggga gttctacatc tRgaagcaaa cagatgataatcactatttt gaaaatacat 64261 aaatactcac tgttagagaa gacacacaaa gaataaagagaatcaagcct tattatttca 64321 gaaaactaaa agaaatgata aacaataaga gaggaagaaaggaagaaagg gacatataaa 64381 acaaccagga aacaatgagc aatatagaaa aaccaaaacctcacatgtga ataataaccc 64441 tgaggtaaac aaattaaatt ccccatttaa aagatatataagtcatataa aagggaacct 64501 ccattagatt aacaacgaat ttgtcagaag aaaccttacaggccaggaga gaatgagatg 64561 acatattcaa agtattgaaa caaaaaaaat cttatcagccaagaatacta tttccagcaa 64621 agctattctt caaaaatgaa gtagaaataa tggcgttcctaaacaagaaa aactggaaat 64681 tcatcactac tacactggtc ctacaagaaa tcctgaaggaaattctatac tttaaagcaa 64741 aaagacaata tggtccatca caaaaacaca cacaagtataaaactcactg gtaaaccaaa 64801 tacacaaatg aaaaagagaa aggattcaaa ttttaccactacataaaatc aacaaatgac 64861 aattataaac aataaaaaat atagaaaggt aaaaggacatatgaaataac cagaaaacaa 64921 ttaataaaat gacaagaata aatcctccgg tagtaataagcttgatggta aatgaactaa 64981 aattttcagt ttaaagatat agactggctt aatggattttaaaaagcgtg acacaagtat 65041 atgctgccaa taataaagta aatttctctg taaagacacatggacttaaa gtgaagggat 65101 gaaaaatgat attctatgca aatagaaaca aaaagtgagaagtactatac ttgtatcaga 65161 taaaacagat tttaattttt aatttgcaaa cagcaaaaagacacaaaaaa ggtcatcatg 65221 taagataaag ggatcaattc agcaagaggg ataatacaattctaaatatt caaacaacac 65281 tggagcaccc agatatataa ggcaaatatt attagacataaagggggaga tagattccaa 65341 cagaataata gtgagggact tcaatgcccc attctcagccttagacacat aatccagagt 65401 gaaaattaag aaataaacat tggattttaa ctgcactttagacatgtctc aaaaaaaagt 65461 atacgaatgt ccagtaggta tataaaaaat gctcaacatcactcatcatc agggaaatgc 65521 aaattaaaaa cacaatatca tccttctcca gttagaatgggtattattaa aaagaaaaaa 65581 aaaacagatg ctggtgagga ttcaaggaaa aactcttacataccgttggt gggaatgtaa 65641 attagtacag tcactatgga aacaatatgg agatttctcaaaataactaa aaatggaatg 65701 accatatgat ccagcaattt cRcttctggc tatttatccaaagaaacaga aatgaggaca 65761 tcaaagggat acctatattc aaatgttgac tgcagcactattcaccatag caaagatatg 65821 gaatcaacct aagtgtccgt cagtggacaa atggattaaaaaaaaacagt ggtatatata 65881 cacaatgtaa tactattcag ccataaaaaa agaataaaatcatgtaattt gtagcaacat 65941 ggatggaact ggagggcatt atgttaaggg aaataagccaggcacagaaa aacaaacagt 66001 gcatgttctc acttatgttg atcacacaga ggtagagaatagaataatgg ctacaagaga 66061 tggaccagtg tttgtgaggc aggaggacaa gtatgaagagaggttcatta atgggttcaa 66121 acatacagtt agaaggaata aattcaaata tttgataacagattagggtg actatgttta 66181 acaatatttc aaaatagcta gaagaaagga cttgaacagttccccaaaca tagaaatgat 66241 aaatactcaa gatgacagat accctaactt gatcattatacattctatat gcttataaca 66301 aaatatcatg tgtcYcataa atatgtacaa acattatgtatcaattttta aaaatcagga 66361 ttgatagctt tactgctgaa ttctaccaga catttaaagaggaattgata ctaattatcc 66421 tgaaacaatt ccagatattg cagaggaagt aatacttccaaacgcatttt acaaagccag 66481 cattaccctg attccaaaac caaagataca acaaataaaaaagccacaag ccaacatccc 66541 tgatgaatat caatgcaaaa attcaacaag attttagcaaagtgaacgca gcagcacatg 66601 aaaacgatca ttcactatga tcaagtgggt tttattcaacatatacaaat caataaatgt 66661 gatgcaccct attaacaaaa tgaaggacaa aggccatatgatcatctcta tagacacaga 66721 gaaagtattt tataaaattc aatatccctg cataagaaaagctcacaaga aattagacac 66781 acaagatatg tacctcaaca caaaaaggcc atatatgacaaacccacaRg taacatcatg 66841 cagaataagg aaaggtgaaa gatttcctct aagattaggataaagataag gatgttcact 66901 ttcatcactc ctattcaaca tagtattaaa agtattagctagatatacta ggcaagagat 66961 agaaaYtaaa Maaattgaaa ttgaatagaa ggaagtcaattgttcatctt ttYggatgac 67021 atgatcttat ttatagaaaa ctctaaagac ttcaacaaaaaactgttaga ataaatctgg 67081 taaagttgca gaatagaaaa tcaatgtaca aaaattagtagcatttctgt atgctaatgg 67141 tgaactatat gaaaaataat tcagaaaaca atttcatttacaataaccaa aaaagaataa 67201 aatacttagg aataaactta accaaggaga taaaagatctctaatactgc aaactatgaa 67261 acattgataa aagaaattga aaaagatgca aataaatgaaaagatactct atgtttatgg 67321 attggaacaa ttaatattgt ggaagtggcc atactaatcaaaatgatctt cagatttaat 67381 gcaatcccaa acaaaatacc aatcagcata cttcacagaaataaaaaaag cacacacata 67441 ttttccgtct ttgagtaatg tatgctctga aaggggaaaaaggcattgag atacaaaagc 67501 acattataag atagaaagct ctatgtgcca tttcctgtcatttaccttga tattagatcc 67561 aggaaactct ctctattttt aggccaaatt attagcaaacttaattgccc ttcgccatgt 67621 aatttaacat aaaacacagg ttctgggaat taagatgtaaacatctttgt gaggcctata 67681 ttttcctcct gcagagagaa caggtaatct tacatctctcaaatttgttt cagttgtgta 67741 tggagacagc tatccctgat ttggtaatgt ttaaactagacttttgcaaa aggatatgta 67801 tgagtttata taaaatttga tagtattgca tgtatttatactgtgcaata tgtatatgca 67861 atagtttaag tgacaaaact aagtggtaca aatttaccttgaggttggct gcatttgtaa 67921 gttggggcag tcacttcttt ggagtgttgt gcagacttgggaatgagaag ctgtagattt 67981 cattctaact ctgccactta cagcaggaca accacttgcaaagcccagct ttctctatta 68041 aaataagacc ctaaatcttc ccaacaccaa taatgaagatgacattctca gatcaggaaa 68101 atgaagggtt cttcagaagg atcatcactg tgtcaggggcagcaactgga gatctgaaca 68161 aaatagctac ctgggcatcc aatccctcta ctgtgaaatgcatgattctc cagacctttt 68221 ccaacacgca tagagctttc tgatcgtttt agtgcctactcttaagtatg aattgaatca 68281 aaggttgacc agacaattga agaaattctc catcaaatgagactcaaaga atcagaacaa 68341 agtgtaattc tgaaggaaaa gaggtaagat aaacagcagaagaaacgttt acaagactat 68401 aattaatata ttctgatata accataaaac ataaaaggatgctatcaaat agaagattga 68461 ccagagaaca caaaagaata tgagcaattg taaattaaattgataagttc aataaaatgt 68521 aaagttaagg aaatccccta tgaggtaggg caaaaaggcaaagatatggt ccataatgtg 68581 aagacaatta aaaaattcac ccaacatgaa ttttagaaaaagaaaacaga gtatatattg 68641 ggaaagacat tatcaattaa atactgccat acattacccagaacttatgg ataattctct 68701 ccataatgaa aaaagccatt gattatctaa aagaccagatttaacatttt caggccccag 68761 tgcaggaaga caaatggagg ctcacatact ctatctctgaataaccatac attataaagt 68821 aaaatcgacc ctaggttcag gttaggtttc catccaagtcctgaagcctg tcccttggag 68881 actgcccttc taacctggcc tagagacaga agaatcatgtgtttccctgc accaacactg 68941 actgatcctg caagctgaga ggtgggaaaa tacggagtggcagggctgtc tgctggagca 69001 gtgggcagga gctgagatga ggcccagcac tgacctggccaagagacaga agaatcatgt 69061 gtttccctgc accaaccctg actgatcctg caagctgagaggtgggaaag tatggagagc 69121 ctctaatgag ttttcagttc agcaaatcta gttaccagtccccagatttc tgtttgattt 69181 ttcaaaatta tttcaatctc tgttaaaatt ctctgataaatcaataaatt gctttcctat 69241 gctatcttgc agatatctga tttccttaaa attgttattttgaattcttg atcagagagc 69301 taaccaactg tcatgtcatt ggggtcagta actagtttggtacctttgtc cttttgggaa 69361 ggccatggct ctttgcttgc tgttgtttcc tgtgggtatacttctatgtc tttccataga 69421 agggttatta attaattcca gtttttcagg tctgggttgtgtttgttgtt attgagtcta 69481 tttgcttggc aaatcctcac tgctgggctg ttgcctcccttttgactcta ggtggtgtct 69541 caacctcact tttaccttgt ctttagtaaa cggttggagtattaactgtc cgaaatggga 69601 aatgtcccaa aggaattacc ctggaagtat gagaaacttggctaggggtt atggtctggt 69661 gacctaagga atgtacctcc taaagcatgg tgctattgaacatctaccct gatttggctt 69721 ctcctttggc caagttatga acagtgtcca gaactggagatgatcttccc tcctccctat 69781 tttgtctctt gctctcttca ggaatattcc tccctgaggacagagaatat tcatcttccc 69841 taagggagtc ataatgcatc tcatgggcta aggaagaacaagtttcttgc cagggaatcc 69901 aaggtggtaa ataatctgtt tgagaacatc aatttcactttattcactct aaaatccatg 69961 aattggggga gattctctgc atgtgtggtg ccaggaagaataggaaggag ggacatcatg 70021 gaggtggaag tcccatctcc tactgtcttc ttgatgattttccacttctc tttggccctg 70081 agaaccatct cattttcata cttgagctat gtatttttcctgacatagat ctcaaagctc 70141 tatatttgtt tttggtattc tgtttgttgg ggtagtaagtcagcttgcct ctacagcaac 70201 actttggaac tagtgggtag ctattttgtt gagacaacaatatgctatct ctgaaaccac 70261 actgaccctt cagaaaattc ctccctcttc ctgacctgcaagaataataa tcactgccga 70321 cactttggag ctggtctggg ttcatcccat tccatccagcactgctggca cccaWgtact 70381 tctctgatgg gtctgaggac aaacctacac agcctgtgccaagaccattg ccagtacgta 70441 cctgcatgtg acaccttgga ttcttaggac tggcctacataggccattgc catcaccatt 70501 accaccagca cattcaacct ggaagcctga gggttgtcttgctactgcta ttgctatcac 70561 ccatgccagg cacattgcac aaagtctcaa gaaccactcactcaccaggc ccaccactgc 70621 aacttccagc accacaggaa actccctgga ggtacaataatttacccaac tgaaactgtg 70681 gatattggtg tcaatgtttg ttgtctggag gctcaaagaccggcatgctc attccactgc 70741 tgccactaac atgtgttgca gactggccca tctagtacccctgttcccat caaaacctca 70801 caaaaacttt tactaacaac ctcagtctta gcaactgaggaaatcacaga ccactgatcc 70861 cgactagaga caaagaaacc atacagatat tatgctagtgtatacaccca gaatcaaagc 70921 taaagtgctg tatgcaacca acactacaga tatatctatagaaaaaatag tgttccccta 70981 tgaaagccaa tcaagaagag gctgttactc cagacgtgcatatactaata tagggactca 71041 ataaacatga agatgctaga aaacatgaca cccctacaggaccacaatta ttcttcagca 71101 ggagattgca acaaaaataa aatctatgaa cttctaaaaaattcataaat atgatatcat 71161 agaagcttag ttaaatataa tagagcaaag ataatcaatacaaaaatctg aaaaaaatca 71221 gaatatgaat aagaaattca ccagatacct agatgtcatgagaaagacca aacagatatc 71281 ttggtagtaa agaattcaat gaataaagta aaaaaaaaaaaatattttca aaagcttcaa 71341 cgatagacta ggtaaggcag aagaaaaaaa ttcaccacttgaggactaga tttctgaaat 71401 aaaccagata gaaaatataa taaatcatct aaataaagccagtattcaat ataacatacc 71461 atgtagttac caaaggtttg attctgagta ttccagaatactcaagaaga agagatgggc 71521 agaggcatga caaaaatttt aaactaagca catgtaccctaaaacttaaa gtataataat 71581 aataaaataa aataaaaaaa gaaaaaaaaa taatagctgaaaatttgtca agtcaagtct 71641 agcaagagat ttagacattc aaatagagga cctcaatgatactcaaattg acacaacccc 71701 agaagtcctt ctccaaggca tacagtggtc aaactgtcaaaagccaaaag tcgttaattc 71761 taaaaacagt aagataaaag catcgagtca tgataatggaaccctcatca gattaacagt 71821 gattttcact gtataaactg tgaaagagag ataaaatcatatatttaaag tgttcaaaga 71881 aaaaaaatct gccagccaag agcactatct tcagcacaggcgaacttcaa aatgaagtaa 71941 caataaagtt tttccctgaa aagcaaaaac agagaattcatcaccacaat gactgcccca 72001 caagatatgc ttaagggaat tctgctcctg aaagcaaaagtacaatatct accgtcatga 72061 aaacacaaaa aatttaaaat actccctagt acagcagacaaatgaagact tgaaatgatt 72121 taaattttac tattatagaa aaatactaaa ccacaataataaaaagtgag agaaaaagaa 72181 acaaagtata tgtaaaacaa acaaaaacaa ataacaaaatgaagtaattt ctcacatatg 72241 aataataatc ttgattgaaa attgattaaa ttctccacttgaaagacata gactggctga 72301 gtgaattttt aaaaaatatc ctaactatat gctgcttacaagaaactgac ttcacctaat 72361 aagacacata tagactgaaa atgaagggat agaagaaaatatcccatgta aacagaaaca 72421 aaaacaagtc gaaataggta tattaacatc agataaaatagactctatgt caaaaacagt 72481 aatgagagaa aaagaaggtt attatttaag aatagaaagatcaatttagc atgaggatat 72541 aacaaaacta aatatattcc cccaacacta gagtatacctagatacataa agctatcatt 72601 atgacatcta aaaggataaa tagattccaa tacaatagtagtgtaaactt taacacctga 72661 ctctcagcat taaacagatt atttagacag aaaatctacaaagaaatatt ggacttaatc 72721 ttaagaataa ggggacccaa tatacattat agacatttttatttaacagt ggcaaaacat 72781 accatttttc ttattagcct gtggaagatt ttctaatacagaccgtatgt taggccacaa 72841 aacaagtctc agtacatttt gaaaaagcag tatactttattttcatatac cacaatggat 72901 aaaactaaaa atcaatatta agaggaactt tggaaattgtaagaatacat ggaaattaaa 72961 caacatgctc ctgagcaacc actggttcaa tgaagaaatttagaagaaaa ttaaaatgtt 73021 tttgaaatat gacaatgtaa acacaacata cagcacaaatagtgctaaga ggggagatgc 73081 tctgaataaa ttctttcatc caaaagtaga gagatttcaattaaacaacc cagtgatcca 73141 cctgagggaa ctagaaaact aaaaacaaac tgaaccccaaattagtgaaa ggaaaaatat 73201 agtaaaaata agagcagaac caaacaacta gaaactaaaaaaaaacaaac ccaaactcaa 73261 tgtaatgaaa agttggttta ttgaaacaat aaataaaattcctaagtgtc tagctaggct 73321 atactatata tgtgtgtgta tatatatata tacacacacacacacgtata tatatatatg 73381 ctatactata tatatagtat agatattgtg tatctatacatatataaaaa tatatatata 73441 gacagaaaaa actgaatgaa acaaaatcag aaactaaaaagaagatatta caacttatat 73501 catagaaata caactgtata ttttggacaa taatacactaatgaaaccgt atgttttgaa 73561 caagtataca ctataattga acaccatgga aaaagattaattccctgaca tatacaactc 73621 acacaaaatg agccaggaaa gaagggtaaa cctgaacagactaataatga gtaaagaaat 73681 agaatcagta ataaaaacga aatctcccaa caaagcaaagtccaggccta gatggtttca 73741 ctgatgaatt ctaccaaact tataaggaaa aaggaaaagcattcctcatc aaacaattcc 73801 aacaaattca gagaagagaa tacttcctaa ctaattttacaatgccagga ataccccaat 73861 gctaaaaaca tacacgaaca caacaacaat aactataacaaaaattagag gtcagtattt 73921 ctgatgaaca cagacacaaa aatcctcaag aaaatgttagcaaaccaaat ctaagaacac 73981 atcagaaaga taattagcaa taatcaagtg gtatttatcccagggatgca agaatggttc 74041 agcatacatg aatcgataaa tgtggtacat cacattaacagaataaagac aaaaccaaat 74101 aagcatatga atagatgWag gaaaaagcat ttgataaaagttagcatcca tttatgataa 74161 aagttcccaa cgaactacac aaagaaggaa tatacctctatataatgtaa ccacatgtga 74221 cgaactcaca cctaacatac agaatgggga aaactgaaagccatttctct aaaaactgaa 74281 ataagacacc actcagtcaa cattttactt tatgtcatgtcctagcgaga agaatcaggc 74341 aagcaaaaaa aataaaagcc atccaaattg gaaaagaggaagttaaattg ttcctgtttg 74401 cagacgacat agtcttatat taggaaaaac acactggggcctttcagagg gtagagggcg 74461 gaaggagaaa gaggatcaag aaaaataact aatggtactaggcttgatac ctgggtgatg 74521 aaataatctg tacgacaaac ccccatgaca caagtttacctatgtgacaa acatacagtt 74581 gtacgtctga acttagaata aaagttaaaa aaattgttaacataaaaaaa gaaaaatcta 74641 aactccacca aaaaaattta ggactatcca ataacattgcaggataaaat atcaatatgc 74701 aaaaattagt agcatttcta tatactagca ataacccagcaggaatgaaa aatcagctat 74761 gaaattccct ttacaaagct ataaaaaatt aattactcagtaatacattt aagcaagcag 74821 actgatgttt accagctata tgactgaaaa tgtcaggcaatcatattgag cttttgtttt 74881 ttcatctgaa gagtaaacat aatgcaagga attattaaagggctaccata ggaataaaag 74941 agctaatggt ataaaacatt attagtatta ttagtattgcatagtatcta tgcaatagtg 75001 taagtcagaa actgaaatgg cagaaatcta cttttaataaccatttacat ttaatgtaat 75061 tattggtatt tttgagccat ttttgttttt catatttatactacttttga ttgtttttct 75121 ttttcctgat ctatggattc cttgaaaatg ttttttgattccattttagt tgatctgtaa 75181 attttttgcg tgactctctg tacagcattg ttagtggttgctcYgtgtta cattaYgcat 75241 acataScttt tcacagccta gtgctgtgca cattttaccctattcagtaa aatatggaaa 75301 ccttgctttc ctgtcttttt aactgctccc cctttcccactgtttgtaac agaatcatat 75361 taaatatatg tctatgtgca tttagtgcca cagtaaattttgtcattttt gctttgacat 75421 aaatttattg tcttccaggt ttgcagctca gaaatctgaactgggtctca ctgcgttaaa 75481 tccatgtctc aaggctgagt tccttcctgg agtctctagaagaaaagctc attatcttgc 75541 cacattcctt ggccctcttc catctttaaa gctagcaatggccagttgag ccatttgaaa 75601 ctcatgcaga aaacgtgaga agaaagatat tttgtgtttacctactttgt atttccaatt 75661 tttgtttttg gcttgttgtc attgttgttg ttgttgctcattcttgttgt tgttgctcat 75721 tcttgatgtt ccagaaaaat cctattctat tttttcctttatgattagtg accttcatag 75781 aaccatttta taagataggt ctgttggaaa gMaattctcttagatttttt tttctgtctt 75841 ctaagaatgc ctgtattttc tctttattga tgagtagttatattggacat aaacatctgg 75901 ttgacatttt ttttctttca gcactttgaa tattttagctacatcctatt ggttattctg 75961 ctctctggta ataacaccac tgttactcac attgtgttttccctatagct atggtgtcat 76021 ttgtttcttg ctgctttcaa aacatttttt ttagtttttagaaatttgac tacagtgttg 76081 cttgcatgga ttttcttggg tatattctgg ttggcagttgctcatcttct tgcatctgta 76141 ggtttatgcc cattgcaagt ttggagaatt ttcagccattatgtctttga gttctctctc 76201 aatagcatcc tcattgttct tcccttctga agctcagattttatcattat cacattattt 76261 gttataacct ttcttgttca taacagtatt taaaattttaccctgtgttc gagtatacag 76321 tttctattaa tccagcctca agaatactga ctctttcctccatcatttcc tttctgttag 76381 tgatcacacc aaataatatt ttatttaatt ttttacactacatatctcag tatcaaaatt 76441 tctatgtcat tatattatat agtttctttt ttctgctgggaatttttttt ccatttaaat 76501 tgagagtgta cacattgacc tcataatgga tgtttgtaacacctgcttca gtgagattga 76561 aaatctcacc gttttaatat ccagattgtt tcaaggttatcatctcttga ttatttaact 76621 ccttgagaat tggtcgattg tttctgattt tataaagtatgttgagtaat tttctatttt 76681 atcccacaca caggactttc tctgtttaca gtacacaataagttcaatga aggaagtttt 76741 ggacattgga gggtagaggc cttcttgctt aattgagatccactcactgg ttcagatagc 76801 aggggatttt gtcccactaa catttttcta cttggagcttctgcctggat gatcctttat 76861 ggctcttacg agataaaact ataagtatat ttaacatcatagcatacata gtgtgcactg 76921 aaggggtccc aaaaaattcc tattcacaaa gtcttttttagttttctttt gtgcatcatt 76981 tgctcaaatc tcaattgaat ttgaggactt cattttcctttcttccctac agtaacaaca 77041 cagtttaaca cctgcttaat tagagtctat tacagtacccgggtaggcct gctgtcttct 77101 gactggggat ttatagaaag tggataatac ggctgaggtaaaatcaacga ctacaggcct 77161 ggcgaaatgg ctcatgcctg taatccaact actttcaagggcagaagtgg gagaattcct 77221 tgacacaagg aatttgagat atgcttgggc aacatagtaagacccagtct ttataaaaaa 77281 attacaaaag gatcctggct cggtggtttg cacctgtagactcagctatt tgggagggtg 77341 agctgggagt atagattgag cctgggagag caaggctactgtgagccatg attacgccac 77401 tgcactccag cctaagagag agagcaagtc aaagtctgaaaaaacaacaa tgaaaaccac 77461 ccaaaaaaca aaaaagacta gggccaccaa aaaggctttctttttcactt gacttcttgc 77521 tccagggttt acctttttaa agtgcacctc ccagtgtctagggagtctac agacttggag 77581 tctcttcctt tcttgcctcg gtatcacaag tggctccatcctatatcctt cagcactcct 77641 tccccatttc ccacagagcc aagtagaata gtgatttgaacacatataag caattttaat 77701 ccctaccctc cttgatgctt tacaaaattt tgggatggatctgttaagct tttgtctcat 77761 ttgagtcagg gatatctcag gcccagctca aatccttaaaggaactgaga tcagagtaga 77821 ggaatagaag tgataaggga gatatgtata aagtgtcttcatgcaagtaa acaagacaca 77881 tttaatttca aattcaagca gtgcagacat tgctcaaaaccaattgaata aacatttgaa 77941 ttttaggtct caccaaaggt ctacaacagt gatggagtatctttaattct tctacctctt 78001 gttctctatg gaaaaaagca aatgtaaagg tgtcatttggtaagtttgtt tcaattctac 78061 catcagatac tcagttttca accatttccc tatcctactcattaatgaac agcataaggg 78121 aaattttcat acattttggt caacatacca gctattggtgtgtccttttt acattaactg 78181 aagaataata aatagtcttt acagatgtta cgagattacaaaacaaaaag catccaaagg 78241 atctaaatta gggtgtaatg tggatgccta atggttttctatcagaactc tcaccaattg 78301 ccctgtctga tgagagaaat gagcaggagc tctgtcgtggtgaaggactc tgctgaagct 78361 cccccaggca tttctctact agagttctgg ctttctcaaaatattctcag aataagcata 78421 tattgtcctt ctctggccct tcagaaagtc aataagcaaaatgccttgag cttcccaata 78481 aactcttaca attacctttg cactttcctc atccactcttctttgactgg accacttcca 78541 cctcttggta gccattgctt tgattgtgct ttgtttcatgattgtactga taaagccatg 78601 ttatatctgc tgtcacaatt ctttgatgaa atgcttcaggatctttatcc ccttatttaa 78661 attttcaggg aagcttcttc tcttgtctgt agctgctctggttgccatag ttttggcatc 78721 cacagagtga aaaatttgct caacattaat ttttcagttagaatcatgta agatgaacca 78781 attaaaaaac atgaagggtt ggctattgtt tgtactcttaatcttcattc ctcttctatc 78841 atggtatcaa caagatacat ttgatatgaa tactctgtcaccgtagactt gaggttcagc 78901 accatttcat cccttcttga aataacatat ctacttgtgaactgctgatt tcttcagcca 78961 ttatccaYat gaacttttca gaaagcatca gtggtttYaccttctttcat gcaagcttaa 79021 ccataaattt tctctttgtt ttcactttaa ttttagaaaaatgtattttg ctcttatagg 79081 ggatgttttg aaactcctgt cttatttttc attattccttgaaatagctc ctattcagac 79141 atcttacagc atatcagttt aggttcattt tgatgccaaaaaatgtggaa tccttttatg 79201 gtatttctta atataacata cattttcatg aactttttaaagacctacta cattacagga 79261 aggcctttca ataatataag aattgctaca ggtaacattgaattttctag ttgattaggc 79321 agattaaMtt tctgtttttc tgctgacata aatagtgatgtgggttgaga aggaatataa 79381 taccagtggt caaatattct gcagctatgt ggaaagtcaaacagttgcaa ctaagtgtca 79441 catgctttaa gggagtcata gagtagctgc ttgggcctagcaggtaccct gatgttaaca 79501 ggatgattgt ctctggtttc accaaacatt acttcaaaagagttactgga catgagcagt 79561 tttgcctagg tagcttacag tagaccttca agtaggtaaggaaggtcaga gagaatgagc 79621 agaggtgaat gccagtcaga tgtgcctctt tgagtgggtagctgaggaag ttccagtcct 79681 ggtgggaggc aggcttctcc ttgtctcctg agtccaggaagagaacaggt gatgcttcca 79741 tatatcactc aactcacacc ctattcctga ccctctctaatgtcagaaat gagtgagaga 79801 gatttgtgtt tcaggcaaga ataaatattg ggtatcaacctaccagacaa ccaaaacaga 79861 gattaccaaa gagctgaagg agacactgtg actgacttagaatggagtca aagccaggag 79921 caggactgca gcaggcgctt aggcatctgt acaggagtcaaggacctcag caatccacct 79981 gtcatttaaa atgaatgtaa acataccgtg taccaagtagaatcttgatt tccaccttct 80041 ataacctgtt ctctttaata tcaacaatca ttaatttcagaccaagaaac cagaatatat 80101 tgagaaacac atcaatgaaa caatacattt cccagaactaaaggttaatt ctccatatcg 80161 aaagggccca atgattatcc aaaagcccaa atttaacattttcagggctc agtgcaagaa 80221 gacaattagt ggctaacata ctccaactct taataatcacacattataaa gcaagctaga 80281 ctctaggttt agatcacatt tccatgtaag tccttcagcctgttgcttgg agattgctca 80341 tataagctgg catagagact aaaggtgtct gtgtttccctgtaacaatcc tgaccccgca 80401 atctgagagg tgggaggagg ggggcacagg gctgtctgctagagaagcac actagacgtt 80461 gggctggagc ccaggactga tctgcccaag cttcgagagcccctgccagg catgttatgc 80521 tcccctatgc ctcacttctg agtttggact agtggcaatgtctacacagc tcccaatgat 80581 aaccctactg agcataggac tggcagagaa taataaccagaatatacaaa caaacaactc 80641 aaaaacaaca aaatagtcca ttaaaaagtg aacaagagatagaaatggac atcttttcaa 80701 ataagacata caaataatca acagtatgtg aaaaatactctatatcacta atcatcagag 80761 aaataaaaat caaaaccaca ctatcacctt accctattcagaatggctat gattaaaaca 80821 aacaaacaaa caaaacagat gttgggatgg atgtggagaaaggagagctc ttatacaccc 80881 tgattggaaa gtaaatagta taaactctat ggaaaacagtaYgatttctc aaagaactaa 80941 aactcgatta ctattcaatc tagcaatctt actactgtatctatgcagag gaaatgaaat 81001 cagtatatga aaaataaatc tgcacttcta tgtttattgaaacaccatac acaaYagcaa 81061 agatatggca tcaacctaag taaccatcaa tggatactaatgggtttgta ggtaaagaaa 81121 tcgtgatata tacacaatgc aatactactc tgcaaaaaaaaaaaaaaaaa aaaaaaagaa 81181 ggaacgaaat cactcctttt gtagcaatac gaatggaactggatgtgaca gataatatgc 81241 tatgtgacaa aggccagata cagaaagtca agtataacatgttgtcacta ataagtaggt 81301 gctaaaatgt gtttacatag atatagaggg gagaactatagacactgaag ccttggaaga 81361 gtgagtgggt gggagggaat taagagttag acattaattagtaaatgcaa tRtttgttat 81421 tcttgttatg gaaaccctaa ggccatgatt taaccattatgcaatgtata catgtaacaa 81481 aattatcttt gaaccacata tatttataaa aataaagtttaaaaataaat tgagggtgac 81541 cgggtgcggt ggctcacgcc tgtaatcaca gcactttgggatgccgaggc ggatggaaca 81601 cgaggtcagg agttcgatac cagcctgacc aacatggtgaaaccccgtct taactaaaaa 81661 tacaaaaatt agccaggcat ggtggtgcat gcttgtaatcccagctactc aggaggctga 81721 ggcaggagaa tcgcttgaac ctgggaggcg gaggttgcagtgagccgata tcttgccact 81781 gcagRccagc ctgggtgaca gaatgagact ccatctcaaaataaataaat aaataaataa 81841 ataaataaat aaataaatat aattgaaggc aaatttaagcatattttttc aaaaaagaaa 81901 aagagggaga tggagtagga gaaataaaaa agaaggaagaggaggaggag gaggaagaaa 81961 gagcctgcat ttctaattgt cataattaaa tttccataagcagatactat tttttttaaa 82021 aaaaacctaa ttagaatttt acgggtctga taagaaagaaaatgctaaaa aaaatatgRa 82081 tactcaRtgt gttcctattt tgtttgaaag cctagaaaaaaacaatatca gagactattc 82141 tacttagatt ttttggacaa agaaatctta agatttttaactcgggaaca tttaattcct 82201 cacaatttca tgtctgctgt tctttcctaa aatcattattgtgaagcaga catgactata 82261 tccactagtg tttcatgtat gtgatctgct tagtcttcatcctttagggg agccattttg 82321 attattcaaa aatttagaag aggaaactta ggctctgagacttaagtagc ttcctatgag 82381 tccagagtta gcaagacata gttcttaaac tgcaatctgagagattccaa attctttgcc 82441 cattcaagtc aggctgcctc tgatgaaatc catttaggttttcttaagaa tagtaagact 82501 cataggcagg gacagaactg atcagtgata aaatatgggaaaataggtct agtggcttct 82561 ggggaataga ggcatagaac catactcatt tccctagaaaacaaaatcgg ttacttttga 82621 acttgttaaa atttctctca gcttctggga ttttctttcattggtattgt tttgcttaca 82681 ttcataagct gactagaata tcaagaaatg acctgaaaataattgtacaa cttaaattcg 82741 agctatagaa gacagagaag gaaagaaaca gtggaactccttccccaggt ggatgactta 82801 ataaggataa ggaggagaga agcttgctgg aaacatcaaggagaaggggt cctggatatt 82861 cttacaatgt gaacatgggt ctcagattct agagtcccaaacactcagaa agagacacca 82921 ccagcagaaa ttgcaagctg attgttttat tggtataYtgaagttagagc atgatgccat 82981 gtttcacggc atttgaaaca ctgttatctt cttattcacttcctgaaaaa gacaagcaaa 83041 caaggaagat tacagacaac acttgacatg gcaggaaatgtctaatccct cactgggggt 83101 tactgcagtg atggagatgc aagcccctac tcttgccctctatcccttct acagccccct 83161 tctccttagt aatattttct ttgttcactc tttaagccattgcacataat gtgatttaaa 83221 gtgttgctta gatgagaaag agaaacaatt attgttatgtgttcatgctt tgctgctgtt 83281 ggttatgtaa gggctatttt tacaaagatg ggcactgcaacatatcatat aatagtgaga 83341 atgtgaatcc Mgccttaata tccagtgaca gggattggttaaatagatta cggaacaacc 83401 aaacaataga atagtataca attgtaaaaa caaactaaccaRtaaacact gaagagatct 83461 aaatgaactc aKgagaaagt tttctaaaca ataaatttttccagtgagta agacacaaat 83521 ctgaatatac agtatggtat aagtgcttgt aataaaagcattatatatat ttatgtagca 83581 atatatattt caaaaagtag attccaaaga gtaaaaggtgggggttttga gaaggataga 83641 agggaggaaa gatgagttag gctcttacta tttagctctgtacatttcgg ttcagtgaga 83701 ttagtttttg ttagcttaag catgtatgac tttaaatgtaagtatactaa aaatgagata 83761 ccattttaca actgaaagtt caaggaccac ttagcaggaatactggacct gaaagcctga 83821 tgtgaggcag gactgagcaa agcatgttca ggtgaaaactttaaatggga gtctgagttt 83881 gaggaaagaa aatagagaga gccaaagggg gaacatggttctatgtcctc attgtcaacc 83941 ctacttaaga catgtaattt gaagaYagtg attctcaaaatcagaattgc cttcaagagc 84001 ttctgtaaaa cacagggtca cagggtcttg atactctatacaagaatatc tgaataacaa 84061 tttttagaaa tgtgttccag gacagggcaa tattaacaggttttcagagg attcattggc 84121 acaatgaagt tggagaactg tagcagttgg agcctttgatggataataaa ctggaatcgt 84181 acctgtcatt gaatcctaga ttactgagga gactgcccctgtggaggtcc ttgtgggcgg 84241 cccccttggg gaggtggtcc ctggggcttt ccaggaggaggtgggggagg accttgctga 84301 tggcctccct gttggggtgg tccttgtggc cttcctcgaggaggacgggg atggcctccc 84361 tgttggggtg gtccttgtgg ccttccttga ggaggagggggatggcctcc ctgYtggggt 84421 ggtccttgtg gctttccctg aggaggtggt ggaccttgttgctgctggcc tccttgttgg 84481 ggtggtcctt gtggctttcc ctgaggaggt ggtggaccttgttgctgctg gcctccttgt 84541 tggggtggtc cctgctgagg gccatcatcc tggttcccatcaccagcaga gggttgagat 84601 tgctgtcctc ccaaaggtgg tccctgacgc tcctcatctaggaactgctc agagtctcct 84661 ccatctgtgt gagttgaaac aagaagagct gagctcatgctggaaaaccc tcctgtcttc 84721 atacctctct gtcttcacca cacggccggc ccctctctgcctgacctgcc tctcaactcc 84781 caacctcccc ccttcccaag gcttcctaat tagaactcctcttaatccac ataagggtga 84841 tgaaaaattg aatttcttta ctcatggtcc ccagaatcaaggttgggaga aaactgtttg 84901 tatctttggg gcattggtat tagccaattc ctgacaagaaaatggtgata agaagacact 84961 ggagaactca tcaatttttc agggaaaaat ggagacagagtttactgaga atttattggg 85021 atttacctga tattacgagg ggaacatctt cctggctgacatctagaaaa gaagtacagg 85081 atgatgggaa aagttacttc ctgaatcatt caaggctcatagtgttctac gaggataaag 85141 gacctctgat cacaccctgt gcatcccctt tgtgatctcatcagccactc tctgatgcta 85201 ctggaagtgg aagaagatgt aagggaaagc agtattgttactacactgag cgtcaaccag 85261 gaactcaaca tagaagggcc cctgtttgtc ctcttttgattccccaagcc ttgatgagga 85321 atgaagatac aataggtctt ctgatcctcg gcatgaaaattctgcagtcc catctgtttt 85381 ctcatcctcc tctcttccct ccactttcct cctctatagcatttgcctgt aaacccttag 85441 cgcttattta gttaaactgc tctgagtatt tcaatgacatatttggggat tcttctgccc 85501 tctttcatct gtaaatatgt tgtttatgtt tgcaagccttctcaacagga gccaccagac 85561 atagccactt ggatataatc ttacgcatgc cattcccagcacattgaaat actacattca 85621 ggagacagaa aaatgacaat gtttcactct ggttattctctaactctatg tagacagaga 85681 caagtgttta cccaattctc tgcctcggca gtagctctgacatgtgttta tctccttcat 85741 taatgctcat ccactgtaca gcaaggccac catcatccctgtctactggg atcagtacag 85801 gatcttcaca gctgtacttc cctgaatctg tcttgcattctccacatcct tcacagcagt 85861 cccatgtctc tatataatgc aaatctcact ctctcacacccctagtacga attctttatt 85921 ggattctatt tgtgcaacaa ataaactatg aggccttgatgaggaatgga ggtacaatag 85981 atcttctgat cctcagcatg agaactctgc agtcccatctgttttctcat cctcctctct 86041 tccctccact ttcctcctct atagcatttg cctgtaaaccccaatcagag tcacaatatc 86101 ttcccccaat tcatcttacc ttcatttaaa tcctgagctgagctgaaggc cagcagggcc 86161 actgacagca gaatcagaag catcttgcag gaggctctggtgtcactccc aactttgtgc 86221 tgggagaaac gtgtcagctc cctttataaa gacaagcaggacaatggtgc atttgagctc 86281 cctaccaggt gggcctcctc gcctcagaga ctggcttctgctttgcttac ttcaggtcaa 86341 gtgtatccct catttctttt gggactctag ctcagcaggaagggttgggt aggatattgt 86401 ttgtgtctaa ttcctaaaag gcacaactat gacttggacaaatgttttga cggaactgtg 86461 tccaagcagt cggcacagtg tcaggattga actttagacatcttttggtt ttcaatctgt 86521 ttggaaagac tgctattctg cttttcactg tgcttttcatttStgtgtat gtgagtttgt 86581 gtgggtgtgt cggggggtgg acactgatcc tacagctaacagtgaagatg gtcaatatct 86641 ctgactgttt tgatagcctc tttccatctc tcatgatgtgtgtgcgtgga tattttcaca 86701 tttagctaaa tttttcatgt aacagagatg gtcttgcttatctgtgagat gtgtgaggac 86761 agcacactcc caggcacaca tatatgaRag aaaggKgctggcagcctgct caggctacaa 86821 gaattgtcca aacttctgtg aatctcacta cactcaggcaagccttggtg ctatacaaca 86881 caggcaagct aaaatatttg tattttaaaa atatctttagggtttattaa agcaaaactg 86941 aactcagaaa ccaccaagga atttctagac ctgaacaaactgaatatatg gttccctaag 87001 tgagccacac gatgtcatat agctaagacc tccactttctccagaatatt ccatagaatt 87061 catcctctac tttatttctt ctaataaata ttgtaattgtcatcactgat aattaaatgg 87121 ccacaattat acattcatct aagataattt gtaatacctgtccaagcctc aattgccttg 87181 attggtcttc tgcttttact cagtaattat gtcacctgtttcatcagagt gttctttctc 87241 accaaggatt tttatctgta atattccaat actcattctttctcattctt tttctcgcac 87301 ttatttgttg aaattttgta ttctgagtct accttgccatgtttactttt cgtcctatat 87361 cttttttttt ctccctacaa aggtgtcctt tatttatccttctaagtaaa ggtgtgtggt 87421 actcctcact acctttactt tcatttttgt ctgtataatttgacaataaa aaattaagtt 87481 tccaaaaagc cctgtcaggg tcctggtttg gtagctctctcctcagctgc tgattgctcc 87541 aaaaattaag tttccaaaaa gccctgtcag agtcctggttttgtcgctct ctcctcagct 87601 gctgattgct ctgtgatacc tagaaattcc ctgYtcatcaattcataaaa tatactactc 87661 agaatcacca gctgcacttt ccatttcaga ccttgagtaggtcttgtgta atttttcact 87721 ttttgtgcat ggttagtctt tatttgagga tagcttggttggaggcatgg ctgagaagcc 87781 aggtgacaga aaatcataaa gacaaggccc tagacaggtgtgcatgagtg gatcctgtgc 87841 tgggcatatg agggtctggt atggctcttc tgaaaagttgcagataataa tacaagaagg 87901 cttcctagta gattagattt tgctacagac gacattgttcttgtaaggga cccaggcaga 87961 ttggtatttt ccctggaata aatatagacc tatttcaggggggaatgcat tcttaattgg 88021 caattcttct ctggccctgt gggaagtaga tcagctctagctgggccgta tgagttgtat 88081 ctcaggcaca gagtagttgc ttgagcccag cagctcccaatgcgttatgg agattattgt 88141 ctctggtttc accaggtact gtttcccata ggacctggagatgagcagtc ctgcctcggt 88201 gacttcaggt caaccatcat gtcaaaaagg aaggtcagagaaaaggaaca aagaggagtg 88261 ccaggcagat attgcctgat gatgtggggg actgaggaagctgcaggcct ggtgcaagac 88321 aggtttctcc ttggcagcag agtacaggaa aagaacaggaaatcctccta aaggtcactc 88381 agctcaggcc tgctccctaa cctgctccaa tgtcagcaatcagcgagaga ctagtgtatc 88441 aggaaagcat aaatatcagc tatgctctca ctagacacatcaaaaacaga ggtaatggaa 88501 gagctgaaag ggacactgtc tgtgagtgaR ggtgggtgaaagggagacgc aggactgctg 88561 caggagcttg ggtacctaga ggtgggtggc agccctcagcaccacacttg ccttctacta 88621 tagatgttca tattctgagg ccctgaaggg ccagtcttcaacatatgaaa ttttaggatt 88681 catctctcat aacacatccc aacattttct cggtcactgacaggtgttta cctagtggtt 88741 caaaaatcct cgctccctcc aggaacttat gcattagccgatctaattgc ctccactttc 88801 aaaatacgtc ccaaatatga ccacttgcct gatctttactagaaggtgat tgttcagcca 88861 tgatgctctc tcctggagga ctttaccagc ctccagctggtcttggtaat gttcttcccc 88921 cagctcatcc catatcagtt ccctcacaac aatcagagtgatcatttaaa agtagaaatt 88981 aggttatatc acatttgtgc ttcaaatctc ctgttttttcttctgcactt gtgataagat 89041 ccaaatttca tgcctcagct ctttcacacc cacatgcctcactctttttt gaaaaccagg 89101 aagctctctg aatggaacag aaagtagaat cttcttcgacttcaccatgc tctagtttgc 89161 ctggaacact aaagatggtt tgccaggaaa gatgaacattgRcagaggga ataaacttct 89221 ctgagagtca gtgagtttca ttgggtcgct gtggtctggcagtgttatga attggcagaa 89281 ttattctatt aattgcacca actaagcatt ctgataatacagttagcaaa taaacacaat 89341 agtctctgag acttcaactt gctttgactt gtctaaaaagtttggtgaca ctattatggc 89401 caattaacta ttaaaaatat cttctatatt tttaaatttgatgttagtgg tccataaata 89461 tatatatttc acaattaatt tagcatattt cttttttttttttttgagat ggtgtcttgc 89521 tctgtcaccc aggttggggt gcagtggcat gatctcagctcactgcaact tccacctccc 89581 gggttcaagc aattctcctg cctcagcctc ccaagtagctgggattacag gYccctcgcc 89641 accatgactg actaattttt tcccatctct actaaaaatacaaaaaaaaa attataYcta 89701 aagaaattaa gtcacatggc taRggtcact gtagtaaatgaggaaaaatt aagttgattt 89761 tctcttctct ttgtaattcc agtttaaccg gatgttgtcgatgttgtttt caatttagaa 89821 aatatagcaa attgttcatt agccatgagt atcactctgctatccaccac tttctaagaa 89881 ggcgcttatt ttagaacaag gctaacacta aattacaggaaattaagtaa ttgtggcaaa 89941 ctagagaagc atagtatgta taataggtct tactcagttagatccacctg tcaaggctga 90001 gtccctttct ggagtctcta gagaagcacc cattttttgtccacattcct tggccttctt 90061 ccatcttcaa tgccagcaat agccacttga gcctcccatcacatagcatc acgtcgccct 90121 tgcactgact cttctgcctc cctcttctgc agttaatgaccttatggtta cattgagtcc 90181 acctagataa tccaggataa cctttctaaa ttcaggtcaattgattcgca aatgtaattt 90241 ccctttgtca tgtaagttaa tatattcact tgtctgagaattaggaagtg tgtgactttg 90301 ggagactgtt aatcttcata gaacacacag gatggacaccttacccatct agacctggtt 90361 tggctcagtg agagttggag agactcaccc aggcttcttccagatatttg catgagaata 90421 tgaatgagtt tatgtataat tagttgttat acaaatatttgtgacttaaa gtatatatct 90481 atgcaaaagc gtaaatcaca aactgagatg gcaaaaatttactttcaata accattgaca 90541 cttaatgcat tttttgatat tttagagctg tcatattttttgttttttat ttatactact 90601 tttgattgtt ttctttttcc tgatctccta tggggccactgaaagtgttt tataattcta 90661 ttttagttga tccgtataat ttttgagtgt atctctctgaacagtgtttt tagtgattct 90721 tctatgtttt actttacatg tacatagctt ataatctagtggtgtacact ttttaccagt 90781 tttagtgaag tatggaaacc ttacatccct ttataatttttaggtcaaaa tgaatttatt 90841 atttttcagt tttgtaggtc agaaatctga actgaggttcactgagttaa atccatgtgt 90901 caaggctgag tttcctccta gaggctctag agaagaatgtcatttttcca catttcctgt 90961 ctctcttcca tcttcaatgc tagcaatggc tggttaaaacatatgaagaa aactcaagtg 91021 aaaaaggaag atatattgtg tttgcccact ttccccctttctSttatttt tacttcattc 91081 ttgatgttcc acaaatttct cttctattct ttcctttatgtttagagaac ttcatttggc 91141 tattatttaa agataggttt gttggaaaga aattctcctaggtttctagc ttttcttttt 91201 ttcttagaat gcctggattt cctttacatt aatgaaggatatttttattg aacataaaca 91261 tctggtagac catttctact tttgagcact ttaaatatttgtgctccttt ctactgttat 91321 ttctggtttc tggtSataat aaccactatt atttatattgcattttccct ataactatga 91381 tgtcatttcc ttctttctgc tttcaatatt ttgtctttaattttcagaaa ttggacaatg 91441 aattttcctg gaatggattt cattggttgt attctggttgggatttgctc agcttcttga 91501 atcYgtaggt ttatgctctt tgctgtgttt ggaaaatttttgatcattat ttctctgagt 91561 cctttttcaa catcaacctc ttctgctttt ctgaaactcagaatgcatga atagtacaYc 91621 atttgttata gcctttcata ttcatgatat gatgtataattttttcaatt attttaccct 91681 gtattgttta gagtatataa tttctattga tccaacctcaagaacactgt ctttcctcca 91741 tcattttctt tatgctattt atcatgccca ataaaggttttttaagacat tatatttttc 91801 atttctaaaa tatctgtttt cttaatttat aatttataattcctattttt ctgctgacaa 91861 gtttttcctt tccattcatt ttgagtgtct accttgacctcatgaaggat gggtacaaca 91921 gctcctgtaa agtctttggt cattttaata tctaagtaatttcaaggttg taatttcttg 91981 attgtttcat tccttgagaa atggtcgatt ttttgtgagttgttttgagt acattgagta 92041 attttctgtt ttatcttgca cagagggatt ttctctttttgcaatacaca aaaagcccaa 92101 taagggtagg ttcggacatt ggagggtgaa ggcactcatggtaaattatg ctccaccaac 92161 tggctcaaat ggccagggag tttgtcccac taacatttttggtggttttc cctgggtcaa 92221 tctctctggc acttatgaga taatatttta agtatttaatatcacaccat acatacagat 92281 ataaagacaa taaaaagcag tgcatcgaat gggtccaaagggccctatcc acaaagtctt 92341 cctaactttg gtgtgtgtgt gtgtgtgttt gtgtgtgtgtgtgtgcactg tttgttcaat 92401 cctcatcagt tgaactcagg cacttctttt tcctttttctttctaaacct gcttgttaga 92461 ggctgtcaca gtagccagaa ggccctgcta tcttctatttgagtatttat agaatgcagg 92521 aaaaaagagg gtaaactata aagaacgact gtaggcctccccggtgcagt ggctcatgcc 92581 tgtaatatca gtacttggag aggcagaggt gggaggactgcttaaggcca ggagttctag 92641 accagcctgg gtgacatagt gagactcctc tgtctttacaaaacactaaa aaattatcca 92701 ggcatgatgg tgtgtgcctg ttgtcctagt catttgggtggctgaggtgg gaggaaaaga 92761 agggaaggaa agaggagggg atgggagggg aggggagggttggctttttt tttttcctct 92821 tggcttcttg cccttctttc tagtgcacct cccagtagggtctcttttgt atttcctcac 92881 atgctattgt cctccatccc tcagcacccc ttcccccaacccccagaacc aagtagaatg 92941 gtgatttgtg cacatatatc caacagggct atactaatttgagtccctac tctccttgaa 93001 gctttgcaat atgttgggat ggttctgtag ggtctttggtgtcccttggg tcaggaagat 93061 ctcaggccca gcccgaatcc ctaaagcaat tgacatcagaatagaggtaa agaagttatc 93121 agggaaatat agagaaagtg tcttcaggcc agtatgcaagacacatttac tttcaaataa 93181 gcattattgc cactactcgg tggttaactg aataagcatctgaatgtaac aactttggga 93241 gatccttaat cttttcacct ttccaccaaa ggcctgtaacaactttggga gatctttaat 93301 ctgtctacct aatgttatct ggagaagaca gctaaatctaaaagtgtcat ttggaagtct 93361 gtttcagttc taccagtggt actcaatttt caaccacttcccaatcctcc tcattaatga 93421 gcaacataat agaagaatat ttatttacca attacaaacattttagtcaa catgttagct 93481 accagttccc atggactttc agcaaatcct attaatcagcttgtaggatc cttcactttc 93541 tcctcagaag gccaggtctt tgggctgtgt tccatcctcgttgccaaaat tattataagc 93601 taacaactta gcttgtcact tgttgtactt gcagaaggcaaaagatggtt tattattcac 93661 tgaagtacta gcagtagtca gaggaatatc atcgtactatttcttcaagc ttcaRtcccc 93721 tcacagggat aagatgagaa actgatgtta catgtgcaagtagtggtgtc tatcaaaaga 93781 gaaatctcaa aattaggaaa ctccagtctt aaaaactttgatcactgtga gtaacctgtc 93841 catcttgcct tctgaagaag aagctttaca ctggaatgtaagcaaatatc tctggagaga 93901 agaagaagaa gagaagagtc tttatcttta aacaaatctgccttYtgacc aagagggata 93961 ccctatttct aaatcccaag gctctttgcc atgacctggtcttgggcaaa gtgaacgaga 94021 ctcctagggc acaaaacgta aaaaggcatt caataggttgcagggcccta aaaccaagtg 94081 gctcctcaaa ctttgcaccc tgcatgcctt gctcacctgatcctagttga ggcccttctg 94141 tttgctcatc aaacatactg tttgctcaca agataagagctagaccatgc agaagtgcaa 94201 tacattcatg gagaattttt ttcccaacat ccatYcccaagatcaaggtt acacagttgc 94261 caaagattgt agagtattct gaatatatca tctattttcttcctggacag aactaaatcc 94321 atcagaagta acttttttaa aaaatgtttc tttcttttgcatacaatttg tttttatttt 94381 agattaagag atgagtccag aggtaagggg gacaggagaactgtgacatc agggatgaca 94441 ctaccctttg agagcactgg agcagttaga aaggattgaaagtgaataat cttatttcat 94501 aaggggtatg tcattttatg attcaaatta agtctgtgattatcacttag caaaattgct 94561 aaaatcatta tcagaatatc ccatatcatg ataggaggaagaaaaagaag taaaatctaa 94621 aattgttatg gaggtaaata gaagactgcc ataaacaagctatctacaga attatggggc 94681 ttaggaattc acagtaccct ataaacaaga acacagctttgagaatttct atactctaat 94741 ttttaaaaat gaagtatatt ttgtgaattt ttcacccacaaattatttta actggaagcc 94801 atcacagctc attaatcagc aattggtgaa ggactaaagcagcccaaatc attcctgtct 94861 caaagtaagg cctgcttgta agcaagtgtg aactggggatgtatgtgcag aaatcagagc 94921 ctaagtatgg taagctggac atttcagaga taaggaaggcagctggtgtg tgtgaaatct 94981 tatatcaata actgatttta ttttttcagt cctaattgctattatggaat ttgtgctttt 95041 cattattaca tcttcacaaa gcatgatttt taactttagaacaatttaca ttttatgctg 95101 gataattctg ttgtgaggga ctgtcatgtg cattgtgagatattgagcag catccttgtc 95161 ctcgtacagt tatcaggtaa aatacagagt gtgtcagttcaatttgaatt tcaaataagc 95221 aacaaataat tgtcattgtc tgtgtatcca attatcaatgctgcaggtct gtaattggtc 95281 ctccatagca cacttacatt atgatgtaaa ctcatattatggttatcaca agtaatactt 95341 atttataaat ttattcttgt tgttttttct tttgtctgaaatgtaaattt aactaggcct 95401 cctatatttt tatttgctta atctgttaac attacaatagatgccagcag catccgtcca 95461 attttaactg aaatgtctcc aggcattatt aaatgtcccctaggggggca aaattgccct 95521 gtgtggagaa ccactgtcct aaagggattt catcctttgcacatggaaat ctcgtggtct 95581 attctctgga tattttaatt ttattcacac atttaaaatttcgttttagg cctgcaatgt 95641 ccaggttatt tttctagggt ttactttttg ttctatcgattaccatataa ttaacttttt 95701 aaaatacttt ctgcaatgtt gaaaatttgt cttttgtctttactgctgaa aattatatta 95761 tttgcttgca atatgttctt ttttttctta gagagaacgtaattgttctt caaaatctgc 95821 ttccaattat ttcattttaa atatttatta Ytgttaccttaaacttgcga ttgtgttctt 95881 ttatttaacc tgctattttt acatgctttc tcattacatttttaagagga gacgttctta 95941 ttaaattcaa ttctgtaaac tgattggtta tccctgatttgtttttcttt tcaaaacttt 96001 atttattttt ctttctttac acagtgactt tcatcaaatgagatcctgta tttgttacta 96061 tcgtcttggt cttctatatt tttatttact aaacctggtaactctacact agatgccagc 96121 agcagtcctc caattttgct tacttaaaat cttactgattttttaatacg tgtccttgcc 96181 ttccttacac agttaaccaa ttgagtaaaa ttttattgttagtccaaaga ttcagattct 96241 acagtacaag tctgttgtag atggtcaaga ttgtaacatttttgatggtc aaagttgctt 96301 agtgttttta actgaaaatg actgaaaata ttaacagaatagggcttata tttggaaata 96361 actcttagtg atgtacattt caattgactt tgtttcatatcctcaaagtt ttcactaagc 96421 tgtagactct actaactatt ttcatttgag ttttcctttctagttttaaa ttatcaattg 96481 atcatcattt ttaggatagg aactaaatcc ctatgtgtttgtacatataa atctgatttt 96541 atatacactc tatacacata catagatatt ataattcttgatgtatttct attctattct 96601 aatgcttatg caacatatta ataaaattta aagatagagtaatctattat attgatcaca 96661 gtgcctaatg aaaaatgcat tttcaatata ttagaattttactattaaat acataatctc 96721 catatatggt gcaaacaagg tagcaatctg ttctgcttaatttaaatcag caagtatttt 96781 caataatgct caatatatca tagaacataa tgagaaaataagaggtaggt gaaagaaaca 96841 aatgtgtagc cctcctgttc ttcttccttt ttttctttctgagaatatca tcactcatat 96901 caggcatgtt tataaaaatg agagattatg tcctttttggcatacttcat cttcttcagg 96961 acacagagag aagcttgctt cagtttgctg tcccgtaaaattagaagaaa tgaatggcca 97021 gatggatgga aaaataccag cacactacta aaagtccttgccgcaacgaa gtctagaaga 97081 ggacaggcca atgttagtat gatactgatg aaataatgcacaataaagag gagtagaaat 97141 gaaattatag cttttatagg ttttacatgg gcttctgtgctgggatccct ggaataaata 97201 ccatgtagct tcatctgcct ggtgtggctc cataaggagaggattaagag aagaatggaa 97261 gccagtgttt ccacaaaagg aaggatgaac atcatctgaaggagaaacag agaagtgaaa 97321 tcatgcatgg tatccatata attaaatgtc aagttgccttttattgttac ccgttcttag 97381 attaaactat taattattat ttccttcaga atggaagttgtgcagaaaga gatcgttgcc 97441 tctagtacaa taaagagaag caccttgtga attttccatttcatccagag gaaaatggga 97501 ttggagaagt tggctacctt gaggaaatag aagacactgaggcaagtggt acaggacagg 97561 cagaaatagc tggatcctgt ccagagaatg tcaaaacttactgcaagatt cttagaatca 97621 ggcattttct catagcctat attgaaagag gtagctaaaattgttatcta caggaaacat 97681 atcctggaga tggccaagca gttgagaata aaatcaatcagggagacctt tcagttcctg 97741 attcagtcaa tgatattaac caatacaatg aatcaatttcccacagtccc cattatgaat 97801 tacactacaa gaattcttat aaacagtgtc tcccaaatgcttgacatgcc tctacatagt 97861 gaatctctgc cttcaataca ccattggctg attgatcctagctgcactgt tatagattta 97921 tatatccaaa ggagtgactt ctttctgttt tattgatggccattggtgta gtctggagag 97981 atccaaggtg tgttctaaaa ttagatttca actaaattgtaaaatataca agtttttttc 98041 ccttgagggt atcagctagt gattttcaag gcaaatattatattccctgc tactattgac 98101 tctggttgta ttttacatgg aaagttctgc attcaaatttgaaccctcat ttgttaacat 98161 gcaaaattga agtagattct cctttatagt tttacaatgccatcttttgc ttaaaggtta 98221 ttcatttgta tgatgtatcc ctttgattca tctaccaccctgggctagaa attctaagcc 98281 cccatataga aaaatgcagt atatatttag agtttatgatcagcatattt cttcattatc 98341 aacctcacta atgcagatta tttataaaat atgcaaagtttaagaaaatg gagaaaactg 98401 ctctctgata taataccaaa tcttttagag agtatggaagtttttggcca ggcacggtgg 98461 ctcacgcctg taatcccagc actttggaag gccaaggcaggtggatcacc tgaggtcagg 98521 agttcgagac cagcctggcc aacatggtga aaccctgtctctactaaaaa tacaaaaatt 98581 agctgggctt ggtggcatgc tcctgtaatc ccagttactcgggaggctga ggcaggagaa 98641 tcgcttgaac ccaggaggca gatgttgcag tgagccgagatcacactact gcactccagc 98701 ctgggtgaca gagtgagatc cgtctcaaaa aaaaaaaaaaaagagagaga gagtatgcaa 98761 gttttaaaac atacgataca tactttggca cttcttctattgatatctta ggagtctgtg 98821 tactctgtcc ttaaatctag aatggtttgt gactaattcaactaataaaa atgctataat 98881 aattctaaag ctaaatcatg aaaagccatg cagttttttcttaatcactg caatatttac 98941 tcttggagtc ctaagccaca tgtaagtagt ccaactactctgaggtcaca atgtccaaac 99001 ccacatgcag aggccatgtg caggccctct ggctcattatcccacgtgag cccagctttt 99061 acgtcctttt accccaggta ctaaacctat gagccaagaaagcgatattt gacctgtctg 99121 atacggtaga tactagccac atgtcacacg tggctttttaaattcacatt aataaaaatt 99181 aaataagatt aaaagtacag agcctcagta ttactaaccatatttcaaat gctcactagc 99241 cacgtgtgac tattttatag ccaacataca taggggatatttctatcatt gcctatcatt 99301 gacatttcta tgtatttctc agctccgcct tagataagttcagcccctgg tcacttgaat 99361 tgcccccagt tgttcataac tttctggcta aaggcttagacattctggaa caaaaagaag 99421 ccatccctgc tatgtcttga acaaaaccct aacctacagaatctttaagt gaattagtct 99481 attcttgcat tgctataaag aaacacctga aactgggtttctgtaagagg tttaattggc 99541 tcatggcttc acaggctgta caggaagcat ggcggcatctgtttctgggg aggcctcagg 99601 aaatgtataa agatggcgga aggcaatggg gaaatcagatacatcttcac atggttggag 99661 caggaggaag agaaagatgg gggaggtgcc ttacactttcaaacaaccaa atcttgtgat 99721 aactcactca ctcactattg ccacaccacc accaagggggaaactgcccc ccataatcca 99781 atcacctccc accaggcccc acctccaaca ttaggggttacaattcaata tgagatttgg 99841 gcgaggacag aaattcaaac catatcaaca agcacaataaaatggctgtt attttacacc 99901 atgaagtttc tgggtgattt tattatgcag caatagagcatcagaataga attatttagc 99961 atatgaatgg tatcaatatt ttgtcatttc taatttccatgtctgttttt ctcttctatc 100021 ccaaataaag gtatccatag catctttatt ttcctcagtgtaaggggaac ttgtataaat 100081 tttctataat tttttcatac gttgagctga acttaatcttaattctgatg aggtcaaacc 100141 aggaaaggcc aattagtcat gttctttgtg taatgcaactgctgcatttc cctcccaaag 100201 tagctagtcc aaaatttagt gacattcaaa accaatggagatataatata ggcctaagag 100261 gagtcaaaga aatgaatgag ttggacagcc caccaaaagatacaccctag gtaaattcac 100321 aaagaaaact ccatgactaa aagaaaggct aattgtgataggcaaacatg aaatgcaaac 100381 aaaactgaaa ctgaaataaa caaagggaaa ttcaggaaaatattatatgc tgaaaaaggg 100441 ttattttatt tgtcttattg aatatcttct ttgttccagccctctaggag gtgggatgaa 100501 gcattaaata caactatttt taaatgccct taataagttgacattctgaa aagatgaact 100561 ctaaacatat aactaataaa atatatatca tacagatataaaatatagtt tataagaagt 100621 tgcaaatata agtagagagg gtcaatagaa agcatgtcagaagaggggtt gaaattttaa 100681 ataaggtgct tagggaagga ctcattgaga aggtgatgtctgaacaaaga cctaaagtag 100741 gtaaagaaat tatccatgtt ttgtctgggt aaagtacattccagacacac ggaacagcat 100801 tatcagtgtg ctaacattgg atgtacctag tgtgtctgaggaagaacaag gaaagcagtg 100861 tgactgttgc ggagatgaga gagtgagacc actaagagtgaagtctcaga aagaagaggc 100921 atgtgcccag aaggcagcac atacagtcaa aagagattattttagaacgt taatatttaa 100981 cgcctgccct gctgtgtttt gtacttgcgt ggtgcctactgctcctctct tgtggctgat 101041 ttctcccttt tggaatgaga atgtctatcc aatgcctgtacaacaactgt atcttggaag 101101 gaaataaatt gttttttatt tcacaggctc atagctggaaggaacttgac ttccagtctc 101161 aaatgagact ttggacttca gacatttgaa ttgatgcagcaacgtgttaa gaattggggg 101221 actattgttg ggatggaaag attacatttt tttttatgtgagaaggacac gagttttcag 101281 gggaccaagt cagaatgcta tggtttgtac acggtttgtttgtccccatc agaactcata 101341 ttgggatttg atccccaaag tagtggtgtt gggaggttgagcttagtgag aagtgtttga 101401 gtcatagggt tggttccctc atgaatgcct tggtaccattttcaatgtag tgagtgagtt 101461 ctcactctca caagactgaa tttgttctag gggaatgagttgtttttcat gagagtgaat 101521 tgttataaag ccaggatgcc cctttggttt attctctcttcacatgtgcc cgcttctgct 101581 ttgaccttct ccaccatggt ttgacccagc acaaaagctctcagcagaaa ctaagcagat 101641 gctaatgcca tgcttcttgt acagcctgcg gaaccatgaactaaataaac ctcttttatt 101701 tataaattac tcagcttcag gtattccttt ataccaacacaaacagacca agaaattgag 101761 gaataatatt tataaaatta tttcagtgaa ttaaattagataaaatggac aaattccctg 101821 aaagaaatta ctgaatcagg cacacaaaga aatagaaaatcttagtgtct atttttaaaa 101881 attgaattct taatttaaaa ctttcacaca aagcaagggatgacctgaat gtttattttc 101941 atcacatatt taagaagaaa aaaataccaa cctacataaatttttcaaca aagtctcata 102001 aaggttaact ctgtgagctt tatttcaaga aggaaagtgggaatctacca gaatcataag 102061 gacacacaga tcccagggag gggaacatgg gcaaacagcccccatgatgg cacccagtta 102121 aaaaagtgaa tgaagctcca gtaggtgaga gaggcagagagcctccctct gtgactcacc 102181 tttccactgg ggatctgagc aacccagtgc tcagagggaagagtaccttg tttctccaaa 102241 gccctggaga taacttggag aaaggcttag agacattgtaagggaaagac actgggaaaa 102301 ggttcaggcc ttttctcaga cccaggactg agagcaggataccattttta attcagatgc 102361 cacaaagtca atcattcttt ggtaactaag cagcgtagctgtgcaggcat tttagtcttg 102421 ggccagatat tggagcacct gctttagagc agggtggggacctccatggc cagaattgtg 102481 gggaaaaaaa accttagcag caaacactga aactgtgctatcccccatca caggcctggg 102541 gcaggagggg agctgctaca gccagtttat cctatgtgatgagacttgca accaggcagc 102601 ttagctactt ggaactagtc tgtgtgtgtc attgctgggtaccccaccct gttcccctgt 102661 gattgtggtg cagcagactc tcctccactc tacctctaggcagaaatctg ggcatttgga 102721 tcactcattc acctggttca gcagcttcat ctgctccacccttcttgtgc agagatccta 102781 gaatagggag gccctctctg cttcatgcct aggcagatcccaggattttg gagcacctgc 102841 tagcaaggtt cagcaccctg aaccacctaa ccttccttgacgtaggtcat ggtgcaatgg 102901 ggccctctct gctccacagc caggcagaac tctagatatctgaagcaccc attcacctgg 102961 ctcagcagcc tatcctcccc tacccttcct gtgcagagattttgatgcag ggaggtcctc 103021 tctgctgcat accctagcat tcaaagcacc tgctcacacagactggcaac ctgtgctgcc 103081 tcacccttcc cgttcacaga tccatgtact gggctgatcctctctgtttt atgcccaggc 103141 aaatatccaa gcattaagag cacctgcctg cctagttaagaagcctgagt caccccattt 103201 accctgtgca gaaatcttgg ttggtggggg tgggttctgctccacaagca ggcagatttc 103261 caggtattca gagcccccac tctcctggat taagagtttaggccaatctc tccaatatcc 103321 ctgtgcaaag aacttggagc cgagggggtt acctagctccacccctagac agaactctgg 103381 tcacttggta gccactcagt agattctccc tcagtgttggtgcttgtgtc tgccattgga 103441 agacctatag gtggacctgc cttgtctggc cccacccaccttgctcccca caacccccaa 103501 ggctgagcag ggagttcaca ccactgtgta ctccatgagtcagaccatta tctgcagcaa 103561 cagagagttt cttccagtaa acaaggatca aatatataaccatcacattg tccaaaactg 103621 gctcttacct ataagtgcca tctactggct tgtaggttgaaccgcacagt ctaatataaa 103681 acctgcaacc tgtaagtgca taaggctata gaagctgggcaaaagaccta cccagaattc 103741 tctaaagtca tacctcctaa ggagggaggg ggaatggaaagggaaagaaa aaagaacaat 103801 aatattatag ggaaataaag aaaaaaatcc taccttatggaaataattac aaaaattaga 103861 agtgccagta ttgccagagg aggaggaaag agaacaagaattctaccacc ataaaaaaag 103921 tgaatgtaat gaaatcaaca ccatcaaagg agcacagtacctctccagca atggtcccta 103981 accaaaacaa aagctcagat atgacagata aaaaattcaaagtatagatt gcaaggaagc 104041 tcaatgagat gcaagacaag gctgaaaatg aatacagagagatttctaaa gcaatccagg 104101 aaatgaagga agacataaac atcttacaaa gaaatcaatcagagcttctg gaattgagaa 104161 acttacttaa ggaattataa agtacaattg aattcatataaagtacaatc tctcatagac 104221 tggaccaagc agaagagttt cagaccttga aaactggtcttttgaactaa cccagaaaaa 104281 aaaattaaaa ataatttaaa aatatgaata cagtcttagagcaatatggg attatgtgaa 104341 gcaaccaaac tcataaactg ctggcattcc tgagaaagaaggagaaatag ttaataacct 104401 ggaaaacata tttgaggaaa taatttaaga aaatttccctaatcttgcta gagaggtaga 104461 catacagata caagaaatca agaggacacc tgactgatgctataggaaat gaacatcacc 104521 aaagcatata gtcgccagac tatccaaggt ctacactaaagaaaaaaatc ttaaaggcag 104581 ctagagaaaa agatcagatc acatacaaag ggaaccccataaggccaagt atatagctca 104641 gcagaaacct tacaagccag gagagattgg aggcctactttcagcattct taaaggaaag 104701 aaaattttcc aaccaataat tgtatattcc atcaaactaacctttataac tgaaggagaa 104761 ataaaatctt ttatagacaa gcaaagggta aaggaatttattaccactgg accagtctta 104821 caagagatcc ttaagggagt tctaaacatg aaaatctgctaccaaaatag cacacctaag 104881 cacatagccc aagaccctat aaagaaacca cacaatagaaactacaaagc acccagctaa 104941 caacttcaaa atagaatcaa aacctcacat agcaatattagccttgaatg taaatggtct 105001 aaataccctc acttaaaagt cacagagtga caaattcaataaaaaacaaa agtcatcggt 105061 ctgctgtctt caagagaccc atctcacatg taatgttactgttaggctca aagtaaaggg 105121 ttgtagaagg atctgtaaca caaatagaaa agaaaaaaagaaagaatcac tattcctata 105181 taagataaaa cagattttaa accaacaaca gtaaaaaaatgacacagaaa ggcactacat 105241 aataataaag tgttcaattc aacaagaaga cttaactgccccaaatatac atgcactcag 105301 cattggagca ccaagattca tgaaacaagt acctccaaacctataaaaag atttagatac 105361 ccactcaata atattggggg acttcaacac cccactgagaagattggaca gatcattgag 105421 gcagaaaact aacaaggaaa ttctggactc ttgaaaaaacgctcaaataa ttaggcattg 105481 aaggaacata cctcaaaata ataagagcca tctatgacaaaactacagct aacattacac 105541 tgaacatgca aaaactgaaa gcattcccct tgagaactggaacaagaaaa gggtccccac 105601 tcccatcact cctattcaac atagtattga aagtcctagccaggacaatc aggcaagaaa 105661 aaaaaaggca tccaaataga aaaataaaag ccatagtcaaactatatctc tttgctgatg 105721 tgaatccata catacaaaac cctaaagact ctgccaaaaggctcctggca cagataaagg 105781 acttctgtaa agtttcagga tacaaatcaa ttcacaaaaatcagtagcat ttctatacaa 105841 taaaaatctt caagctgaga gccaaatcaa gaatgcaatcccatttacaa tagcctcaaa 105901 aaaaaaaaaa aaaaaaaaaa caactaggac tacagctaaccaaggaggtg aaagagaact 105961 gtgaaacact gctgaaagaa atcatagatg acacaaacaaatggaaaagt attccatgct 106021 catggatagg aagaatgaat attgttaaaa tggttgtacggcccaaagca atttacagat 106081 tcaactctat tcctatcaag ctaccaatgt cattttgcacagaactagaa aaaaaaaact 106141 attttaaaat tcatataaaa ccaaaaaaag agcccaaatagcaaaagcaa ccctaagcaa 106201 aaagaaaaaa gccagaggaa tcacattacc tgacttcaaactatactata aagctacagt 106261 aaccaaaaca gcatggtaat attataaaaa cagacacatagaccaatgga acagaataga 106321 gaacccagaa ataaagacac acaactacag ccatctgatgtttgacaaaa ctgacaaaaa 106381 taaacaatgg ggaaaggact ctgtattcag taaatggtgctgggatatct ggctagccat 106441 aagcaaaata attaaactga acctttacct ttcaccatatacaaaaatta actcaggatg 106501 ggtcaaaaat ttaaatgtaa gactttatac tacaataatcctggaagaaa acttaggaaa 106561 cacaattcta aacattggcc ttgggaaaga atttatgactaagtccccaa aagcaattgc 106621 aacaaaaaac aaaaattgac aagtgggacc taattaaactaaagaatttc tgcacacatt 106681 gtataaacta tcaacagagt aaacagacaa cctatagaatgtaagatttg caaactatgc 106741 atgcaacaag ggtctaatat cccaaatcta taaggaatggaaacaattga acaagcagaa 106801 aacaaataac ccctttaaaa acgagcaaaa gacatgaacaggcacttttc aaaagaagac 106861 atacgagcag ccaacaaata ttttaaaagg ctcaacatcactaatcatta gagacacgca 106921 aatcaaaacc acaaagagat accatctcac accagtcagaatagctacta tcaaaaagtt 106981 aaaaaaaaaa aaacagatgc tagtgaagct gtggagcaatgggaacgctt gtaccctgtt 107041 ggtgggcatg caaattacgt cagccactgt agaaagcagtttggagattt ctcaaagaac 107101 ttaaaataaa ctaccattca acctagtcat cccattactcagtatggtgt atgcacgcca 107161 cacttttttg gtctaatcca tttttttttt tttgagacagagtttcactc tgttgtcctc 107221 tcactctgtt cacaagaaaa taaatcattc taccagaaagacacatgtac tctcacaatt 107281 tcattggaac actattcaca atagcaaagg catgggaatcaacctacgtg cccatcaaca 107341 atggattaca ggccaggcac agtggctcat gcctgtaatcccagcacttt gggaggtcaa 107401 ggtgggcaga tacctgtggt cagcagttca agaccagcctggccaacatg gtgaaactct 107461 gtctctacta aaaatacaaa aattagccgg gcgtcctggcacctgcctgt aatcccagct 107521 acttgggagg ctgaggcaga agaatcgctt gaacctgggaggtggaggca gcagtgagct 107581 gagatcatgt cactgcactt cagcctgaga aacagagtaagattctgtct caaagaaaaa 107641 aaaatggatt agaccaaaaa agtgtggtat atatagaccatagaatacta tgtagccatt 107701 aaaaaaaaat gaaatcatgt cctttgcagc agcacggatgcagctaaagg ccattgtcct 107761 gagtgaatta atgcagaaac agaaaaccaa atactgcatgtttcacttat aaataggaac 107821 tatacaattt taactaacac tactaagtga tagaattgagaattgtacct ctgactccag 107881 actcagtcct taactattat agtgtttttt gtggggaccatatgagtgca gaggtgaaaa 107941 tactgacaga tattgaaaga tgctattggg taaataaactacaagagagt tcagagaaat 108001 taatgataaa aatgatcata ctactcctct cacttacctaacttttaagc aaacccattt 108061 gggaggtcct ttctaaaaag aaaataaaag agagaaaatgagaatgctca agtagatagt 108121 actattgaaa caggttacta aaaatgcatg tggtatcaccaaagaacctg ttctgacagc 108181 agagaaaatg aaattaggct ttctgtcctt atactcaaggataactgtgc cttattgcaa 108241 atatttattc caactgttcc ataaaatatc tttgagttttgaaagcaaaa aaagttaaaa 108301 ataattttct tacaggaaaa tagttgatta ttgaacttcattaaaagttt ttaatgtcct 108361 ctctttgaaa gacactgtaa agaaaatgaa atgacaagcctctggctggc agaaaatatt 108421 tataaaacat atgtctgata aagaacttat attcagaatgatatggtctg gctgtgtccc 108481 cacccaaatc tcatcttgaa ttgtagctcc cataattcccatgtgttatg ggagggactc 108541 agtgggagat aattgaatga tgggggtggt ttcccccatactgttctcat ggtagtgaat 108601 aagtctcatg aaacttgatg tttttataag gggtttcccctttcgcttgg ctgtcattct 108661 ctcttcactg ccaccatgta agacgtgact tttgtctctgccattattgt gaggcctttc 108721 cagccatgtg gaactgtcag tcaattaaac ctctttttctttataagtta cccagtcttg 108781 agtatgtctt tatcagcagc atgaaaatgg actaatacagagactatata aagaacattt 108841 acaactcagt aataaaaaca gaaagcaatg aatcaagctaagtagttggc aaaaggttta 108901 aacaaacatt tcataaaaag aggttgtatg aatgacccaaagcatatgaa aaaatcatca 108961 gcctcactag ccattaggaa aatgtgaatt aaaactatgatgagatgcca ttacattctg 109021 actagaatgg ccaaaataga aaacaaaaca gagtacctaccaactgttgg ccagaatgtg 109081 aaataactga aacactcgta tattgctggc aatagtatgaaatggtgcaa ccactctata 109141 gaactctggc agttttttct taagttaaac atacacttatcatatgattt gacattttta 109201 ctcataggta tttactcaag agtgaggaaa ttgtatatccagaagaagag aaatacatga 109261 atgttgatat cagttttatt tataatggcc aataattgaaaaccatttga atgttcatta 109321 acaggaaaga gaaatttttt aatgtggtat atccaacactactcaggaac actgctcagg 109381 aacaaactac tgattaatac aacaaaacag aagaacttcaaaaacattat gctgagttaa 109441 agaagcctca catataacaa tacacactct ataactgtatgtgtgtgtgt gtgtgtgtat 109501 atatggcatc tatatatcta tatctataga tatatagatgcataaaattc tagaaatcaa 109561 aaactctctt attgttacca aaagcagata agtagttgcttgggaaaaag ggctggaggg 109621 gagagggtaa actgcaaagg gcactgacat tgtggtcacacaagtgtata cattgtcaaa 109681 actctaactg aaatgtggac taacatgttg cattttattatatgtaaatt atacctcaat 109741 aatatttact ttaaaaggca taatgtaaaa tatgtatgtacacatacata caatataatt 109801 actaaaaaac cgttatcttt ttatgtggca gttactcatattaaactttt taaccctctc 109861 aattctctca acacatcagt atagtaatag tggcaagtccaaacttccct aattctattc 109921 caaataactg ttctaattat ctgtagctca ccatttaaataggagtactt gagataataa 109981 aataattacc taaatcaccc atctcaatga atgatttagaattgaagagc cattgccaaa 110041 caaaagattg tagatttaca attaacatct aagtatgctgtagtatatgt ttttaattta 110101 caatagaatc tgccaataat ttcctcaaat aagtttcaaattcctaataa tgtagaaata 110161 catgtcataa atacataata ttcttaggta agatgctattatagagaaga atttaaaaca 110221 tttacagtga ccttcacaat aaaagaaacc aggggaagcataaaatatct tttaaactcc 110281 ttgtagactc ctgtttctgt ctgcaatatt caataatctgtggtctgaat ggcttatgaa 110341 attgtgagtt tctcatcgtt tagcccatac cttagctgccaccaaaagaa aggcctgtct 110401 taacttagcg tttcctagaa tcagaagaaa ggagtggcttgaaggagaga agactccaat 110461 cgtctcacaa agcatgtaga tcactgtgtt ctgatacagctcagaaatcc atgatatgag 110521 aacacataga aagaaactag catagaataa aaggaatgagatcacaattt tcaaggcatt 110581 tgtatggacc ttggtcctgg ggtctctgtg tcctttgtaattgagttgca ttttctggag 110641 atgtttctgc agggagaaaa ttaacaggag aaaagagatgaaggccacag taaatggtgt 110701 tagactgaac atagtcatag tgaatttgac cgacactgaaaatgtttcaa agtcactcat 110761 actgaaattc caagttgtgt ttctttcata tcggtccagccagtctttta tatgcatgtt 110821 tatttgtatc agatttaaaa ataagaagac caaggttcctagcagtatca tcagaatcac 110881 tttgtttact ctccacttca aatagagaaa agcagggctagagaaactcg ctattttgag 110941 caaataaaag atgctgaaga ttgtagcaag ccagagattgaagtgattag aaactatcca 111001 gctaaaaatc ataattctta atcctgttcc agacacaaatatggctagat aatgcagagc 111061 taaaaaccaa cttactaata tttcccagat cagcccaattctggagattg ccaagataat 111121 gaggagttta tcgactgagg acagctctct tttactgacccagtcaatgc agttgatcag 111181 tactataaat ccattgctca aattcccaat tatgaattctgcaattatta caagagtgaa 111241 gatactcggc agggcacttt ccatgtcaga acagagaaagttcaatgtct aatgtcactg 111301 ctggttattc actgatctaa aatgctattc acatccttgagtgtccagtg gagttcttct 111361 tccttctcct ttttctgctc cttctttcat tgttggctcaacgtcaaagc agaaatctct 111421 aaagtttgct gatcgatctt cacataactg ttctggtgatatctttattt ttcttcaatt 111481 tctctgctga gccctagcta agatatttat gtcttcaccatgggcagaaa tatttcatag 111541 atgattatgc agcaaagtta aactcacatt tgcaaccatgcaaataaaga tatattctct 111601 ttcattgttt tgtacttttt tgccttgtct gagcatagaaaattagattc aaccagcttg 111661 agttctgagg tacaaatatt ataaaaatct gattcatagaatatgtagct aaatgaagct 111721 tttatggcta atagcatagc caatgaagct ttataaaatatgcaaagact tacctatgct 111781 atttcaaaag agtgctcaat ttcttgtgga gctaaagctggatctggtca atactgtgac 111841 taaagagaaa ctcgtttaca aagcatccat ctttctcattccctgcctca tcactactta 111901 gcagtgctca ccaccatccc tccataggca ccaaatgccttcacatttta tccgcttttc 111961 ccctcatatt tcttttttaa acttaaaagt gataaaaaaacaaattttac aaaatcagca 112021 acagctacca aagaaagcag tactatatta catcataacttgcaaaactg aaaaccaagc 112081 aaagaaatag atacactagt tccccttatc catgaggcctatgtcccaag acccccagtg 112141 gatgcctaaa acagcagata gtaccaaact ctatatacaatatatttttt tcctgtattc 112201 acctacctat gataaagttt aatttgtaaa tcaagcacagtaaaagatta caaaaataac 112261 taataataaa atagaacaat taaaacaata tactataataaaagttgcca ggggttgtgg 112321 ctcatccctg taatcctagc aatttgggag attgaggcgggaggaccgct cgagcccaaa 112381 agtttgagac cggcctgggc aacataggga gaccctgtatctaaaataaa taaaaattaa 112441 aaaaataaaa agtaaaaaaa tgtgttaaaa tatatagtgtggtgtctctc actctctcaa 112501 gataccttac tgtactgtac acacctattt ttggactgtggttgaccttg ggtcactgaa 112561 actgcagata agcggggact aatgtgttca ttgtgaagagtcaaggttcc tcaccccgcc 112621 ccactctttc aaaaactacc gaaaaaaagt ggattaccaaaattctgaaa acgtggagac 112681 tcaagaaaga ttgtctaatt caaaattaat ataaataaaacactttatct tgattataag 112741 ataataagta gccatttagc tagatgaata caaagctatttttatttaat aaagatataa 112801 tttgtgaatt atgaatgttt gtattttatt actcatgcaatctttattgg gtgatctaca 112861 gagtactaaa aaatttacaa aataattgca tcatctttaatgatttgcaa gtttcaatta 112921 taacttaaaa taggtagata ttttctattt tgttgttataatggtatatt tgtcaagata 112981 gaatagaaaa tgtctccttt aacagtttgt taggtttgtatatttatgac ctttaaatat 113041 gtagctatgt ggcatgtggt cttccatttg cattcttgacccagggctga agagatcttt 113101 tatacttttt cattttaggg tgttcttcct acctatctagagaaaagggt ttggggatgc 113161 cggtcacaac ttgataatac cccaaatctg tacaaataggcctgaggaat ggtactgtac 113221 ttgctggtat atgaaaaaat accacagttt ttagaacttgtgatataaca catgacaaag 113281 acatagagaa tggataacag gcttgcggtt ctgagctgcattttcctggt gtgtttccat 113341 aagagaaaac atttaaaaat ataaatttaa tcaaagagatgtagtctctc acttctataa 113401 gagaaagaga atagatgcta gggagcatca cagaaaaactttttcagcag tctgaactga 113461 aaaggaaaat gaggcaaagg gcaggaagag gaaacaatgaaaggtttgag tatgtaaagt 113521 attttttaag tcactgatgg atgcagtttg ccaacgttgtgttaggatat ttgcagctat 113581 gtttttaagg aatattgaca tttaattttc atttctcatattgtgcctat gagaatttgg 113641 tattaagatt tagctaacct catatcattt gtttagaaatatttcttttc tctttttcat 113701 ttttgctgcc tattattgtt acatataaca aatatagaacatttaatgaa gtttaaatgc 113761 tatagtataa agtgcacact aatttaaaag cctcccatggcaagaaagag aactttgtaa 113821 accccataac accttatttg ctcctttcta atcacaacccctatcttcat tccagaagta 113881 acaactatct gcaattcaaa tcacgttatt gtttaactttgtatatttta atttgaatat 113941 gtatccctaa gcactagctt ggttttgcct ggttttggattgcacatgaa tggaataata 114001 ctgtacacaa tatttttgaa tttaactatt tttgatcaatattatgtttc taaaattaat 114061 catgttgcat atagagatag atcatttatt caattgctgtatagtattaa tggaatgaca 114121 attcaaaatt tatctagtct agtggaaaca caaagttggtttattttgag tttggagact 114181 atgatgatga tgatgctacg aacattcttt aacatgtaacttattattga gtacatgagc 114241 atacatttct atgatcttga tctggaacca ttccgttgcctatttcttct ggtcttgttt 114301 tggcagttac gactttctag atatttgtca gtttcaattatttatgtttt catgtaattt 114361 tgattataat atcatcttat taaattttta ataccaacagaatatatcct ttaaattctt 114421 catattattt gcttttcttt tcatgattta tcttaccagaagtttgccta ttttgttagt 114481 ctattcaagt ctgtcttggt cgatcttttc agatgtattatttttcatta atttcatctc 114541 ttatattgtt atttaattat ttttacttcc gttgagtatgtttgctgtta tattcctggc 114601 atacgttttc ctcttagaat ttttcagcat ttctcacaaggttttactat attttattat 114661 acttaatttt aaatattttc taaatttatt gtgatttttcttccaccata gactattttt 114721 aaaatatatg ttcgagtttc catttacata aaattttctctgctaaaatt tatgttatgc 114781 atatatggtc aattttgata aatgttttgt gtttgctgaaaataatgtgt atcctgtagc 114841 tttggggtgc agtgtttcat acatccacta gatctatttgtaatcatgtt atttaaatcc 114901 tatttacctt ttctgagttt tgtcttttta ttctattatttcttgagatt taatgttaaa 114961 ctatcctgct atgagtatag gtttatctat gtcttttagaagtcctgtca acttttactt 115021 tacatatttt gatgccatgc tgatgagtgc atagagcgttagaattgtag tatcttcatg 115081 gcaaatataa cctctactca catgaggtga cattatctattctaatgtct ttttccttaa 115141 aatctattta gcctcacgcc tgaaatccca gcactttgggaggccgaggc gggtggatca 115201 tgaggtcagg agatcgagac cagcatggct aacacatggtgaaaccaagt ctctactaaa 115261 actacaaaaa attagccggg catggtggca ggcacctgtagtcccagcta ctcgggaggc 115321 agaggcaaga gaatggcagg aacctgggag gtggagcttgcagtgagcca agatcacacc 115381 actgcactcc agtgtgggtg acaaagcgag actccgtctcaaaaaaaaaa aatctattta 115441 gtatatatta aaatgattac attagcttat ttttgttagtgttttatggt gtgatttttc 115501 taaattttac ttttaacttc tctttgttat taggatttagatatatatct tgtacatagc 115561 atagaggtcg taaaatggga cctagagcta tatgctaacattttattcct tgtaggataa 115621 gaaatgtagt cttgtaggag tcccctctcc cccttgaagttgtatattat gtaaattttt 115681 ggtcaataaa atattgtgga aatgacatgt tgcttcagagtgagagcatt tactcaccac 115741 tataagactg caaatttctc atcttcttgc cttgacactcaaacaagggt caataaacca 115801 tctatcagcc tggattcttt tgtgaccact atgagcacaggacatgtaac agaaatcaaa 115861 aacaaaaaca aacaacaaaa acttctccta cgtggagtccctgagctttg gggatcttgt 115921 tagtacagca tagactaagc tatcttggca agtatgagactggcctcttg aactggtatg 115981 ttactaaaac aaaacaccta acttatgtga cactggcttaatacttgatt cattggcagt 116041 gagtggcaag aaaaaacatt actgtttact gtaaaacaagatagggagtg gcaaaacatt 116101 cgatagcagt cacctgtggt tatgtgggag gtaaatcacatatttgaaag ttttgtaact 116161 ctagaaaaaa aatataattg aatatattaa tggttcatttgacaattaaa aaaattgact 116221 ttagaaaata attgcctact gtctagctaa atagcaaacagagggagatg ctctaaaaga 116281 aaatgatatt tatttgggag taatattgca atgagaatacatgtgccata gtaaattatg 116341 tgtgtattca gggacattag aaaagacaag gatttctaaaagaggaagat tacataattg 116401 ttttgagata attaaccttg gatccaagga tcaggaacaagggtgacacc tgtctaaggt 116461 tagacaagga ggttctgagc agatgtcctt gcagaagtatctttttgtgt agggttgcag 116521 tggcctttgt gcaatgttgt tgttttgtag agtcttttctggtagctctt gttataagtc 116581 gtatttacat taagaactct cccctcatgg ccttctccagctccatttgt cagagtttta 116641 acacaagtga cttcattttg attctgacaa ttttatactactttgcaagc aggaaaaaaa 116701 tatatcagaa agaagagtct agaaacttga gattttgcagagctggaaga ggcaactgct 116761 tctcaacact gaatagtatt tgagtttcta agcctggttaaaactcagcc atggctgggc 116821 acggtggctc acgcctgtaa tcccagcact ttgggaggctaaggcaggtg catcacaagg 116881 tcaggagatc gagaccaccc tggctaacac ggtgaaaccccatctctact attaatacaa 116941 acaattagcc tggcatggtg gcgggcacct gtagtcccagctacttggga ggctgaggca 117001 ggagaatggt gtgaacccag gaggcagagc ttgcagtgagccaagatcat gccactgcac 117061 tccagcctgg gccacagagt gagactccgt ctcaaaaaaacaaaaaacaa acaaacaaaa 117121 aaactcagcc ttaagactag gattatctca agggtatggttgttacatcc atgttaaaaa 117181 ttctgactag attatattta cagaaaaagg ttcaactgacagttactgct ctttcagttt 117241 gacaaggtaa tatcaaggca gagtgattaa atatctggtttcctgaatga agccttacaa 117301 tgctcaaagt atctgatatt aaatccagag agaaaagcagaggggtgaaa aagcaacaaa 117361 acacagcaaa tataagaagc agcagactag acaaaaactgtgacttgttt gcttgtatgt 117421 gaagctgaca ggaattaagt agactaaaaa attgagtttcttaaaaaatt gtgcttctga 117481 aagaaatgcc aacccaaact aggtcacacg tgtcaaagtatattaaaatg gaggccaggt 117541 ctgaagaatc cctaggcaga caaagccagt taggtctcatatgaccttaa aactgattga 117601 tttacaaaag taagcaaaaa ttaacttgag ctattttttataaatgccta tagtaaagaa 117661 aaacagaact taaactcaac caatcaaaga cagccaacaggatagccaaa catttgattg 117721 gttagctata aagttacctt atttcatctg tcctgtctttgcttttcttc tttgtctgtc 117781 ctataaaagc atccccatcg cgttccctcg gtagagctccggaaccacct cagtttggag 117841 ctttccaatt tatgaaccat catttgcaaa taaacttttaaaaaacttta gtgtgcctta 117901 gttgacctta ttaacacatg ttgagctaaa accctgtattagtctgttct catgttgcta 117961 ataaagacat acacaagact aagtaattta taaaggaaagaggtttaatg aactcacagt 118021 tctacatggc tgaggaggcc tcacaatcat ggtggaagacaaagaaagag gaaagggaca 118081 tcttacgtgg cagcagacaa gagagagttt gcaaggcaactcccctttat aaaaccatca 118141 gatctcatga gaattactta ttcattatca taagaacaacatgggaaaga cccgcccacg 118201 attcagttgc ctcccactga gttcctcccc atgacacatgggaattactc gggagccaca 118261 attcaagatg agatttggat ggggacacag aaaaaccatatcaaaccctg ttccagagat 118321 gcagtttaac atattatgag acaaatggga gctcacagacaatatgagaa tctcttgaag 118381 attacacttt ttttcctagt tctaaaatta catcttttttaatgagactt atattcttcc 118441 cccaactcag aacatttcct tgtctttaag ggagacctgccacagataga gaaccataga 118501 aaataacagt aacaaccaca taaactgtca tgttagctatgaaaagagtt ggtggaagga 118561 atgggctctc accaagacag tgaaaaatag aggtaggtggtaaacaaata tgcaaaagga 118621 ccattcagaa acccaagcaa actatcattc tccccattttactggtgcta taaatacctg 118681 aaaaaaagtg ccacagaaat ggattagtgt tatcatcaacaaatatattt gagccgggca 118741 cagtggctca cacctctaat cccagcattt tgggaggccaaaatgggaag atatcttgag 118801 cccaggagtt tgagaccagc ctgaacaaca taaaaagaccacctctctat aaaataagat 118861 aaaataaaat aaaataaaat aaaataaaaa aaatagctgggcatggtggt gcacatctgt 118921 agtcccagct acttagaagg ctgagatgga aggattgcttgagcccggaa ggcccaggct 118981 gcagtgagcc atgcttatgc cactgccagt cagcctgggtgacagtgaga ctcatatata 119041 tgtatacatt taaatatatg tatacatggg tgagactcatatatacagat atgtatatat 119101 ttgtacgtat gagataagct ggtgagaatg aggtcattgaagcagatatc ctgttccttg 119161 ccaattaagt cagtgatgaa ttccatagtt aataccaccatgaaaatgtc ctcctctata 119221 cctggcttct cttcagagag aaaaattcct aattttataattttttttat tttcaactaa 119281 gggattaata caataattaa gggattaaat atttaatatttattgtttca gtacttctct 119341 cttgctatgt ccattaattc cattgctcat tgttaatttgttgttaatta cattaaaatc 119401 agttcttctg taggatcatg tcatattgat ctttatgaaatcagagcaat catttttcaa 119461 atgattcagc tggcaatttt catactaaca aatattttagttttttataa gagccacagg 119521 tgataacttt ccatattcaa aagaataccc agtgaagtctagtgttctca gatgcagtta 119581 cggacagttt tcctttcact gttttcactt gttgcttcaccctaaacttc aatttatgta 119641 cacattacag tccccaatta ggagactaac aggatgttgaagaatggcat tggcaggaca 119701 tttctgaatt cagtagcagg aatgaactca aaaccagcagtactgagtca gttcttgttc 119761 tcttctgact gaattcgtgt tcatggaaat gtttctgcaagactgagtgg tcacagtgtc 119821 agaaggctcc tcaggttttg cctcttcaca catgtccatttcccctttga ccttctgcca 119881 tgtcaagata cagctggaaa gccctcacca gaagctgagcagatatcagt gcctgctttt 119941 ggaacttccc agcctgcaga aacattaggt ctttatagattgtccagctt caatattatg 120001 cagccttaaa aaggaacaag atcatgtcct ttgcagggacatggatgaag ctggaagcca 120061 ttatcctcag caaactaatg caggaacaga aaaccaaacaccacgtgttc tcgcttataa 120121 ttgggaggtg aacaatgaga tcacatggac acagggaggggaacaacact tagcgggcat 120181 gttggggcag gacgggggtg ggagagcatt agagaaaagagttgatgcat gctgggctta 120241 atatctaggt gataggttga taggtgcagc aaaccaccatggcacacatt aacctatgta 120301 acaaacctgc atatcctgca catgtacccc agaacttaataaataaataa ataatacatt 120361 tttaaaagat ccacatgatc aaaaatgcaa aaaaaaataaattatccaac ttcaggtatt 120421 cttttatagc aacataaaat ggactaagac aaaactctgtggtgaaatct ccaattattt 120481 gtaagatgaa gtcgtagaaa tgggtgaagt ccaaaaagcaggttataatc tatataaaca 120541 tttaagactt ttattaatat tgacaaagtc ccctacaaaatgcatgcacc aatgtaagct 120601 ctcactagca ttaaacgaga ctgctcgtaa ctttgcacacattataattc tatgtaacaa 120661 tttacaaaag caaaatgtgg ccaatttgaa atgcaaaatagagcacttgt tattcatttt 120721 accatagtac tatactaaac ttacgttttt gtttaaatgtgaggctgaat tttttatata 120781 tgataatctg gtttttaaaa ttatttttat cttttttataaaacagtaaa agtattaaat 120841 ttttttctac tgtttatatt gcaaatagac tttaaagtttttaatctatt atttaccttt 120901 ctatatttat tttcttgagg cattcagaaa ttcttccagtcatgctatta aatgcattca 120961 ttttaaatgt actctgcctt taatatcatg tttaaacatgtcttcccaac tttcaagatt 121021 acaaaacaat tcttggtatt ttctctgttt gattgtttactttttcaatt ctatatttaa 121081 tcctcttatt tactttggaa gaagaagtat gttacacatttgtcttattt caaattgcaa 121141 tattgttgtt acaatactaa ttattgcagg gttaatttttcttcacatat ttaagatgga 121201 aaaatctatc tcacacaata tatttgtaaa caaaaaaagatatttataaa ctctataatt 121261 tttttgttgc actggagttt cctcattctt tcatgatgttattctatgcc actctttaaa 121321 gagaagtgtt ggaggacatg aaaggtgaga ggatagacatgtaactattt gttggacacc 121381 taacttagga tggacagatt atattatttt tatcagcaaatgtgaactca tcagtctttt 121441 ccttaatgac atgtgcttcc tcaaattaaa ggcagtagagtcttattttg attttcttgt 121501 aaaagtttta aagaattgtt ttcaaatcta aagtgatagtacatctaatg tagtttttct 121561 gtttgttgtg agttagaaat acaattttgt cccaatattaaatgacagag gtctcaatat 121621 cattagttga acagtctacg tttttttttt gttgatttctaatgccaaat ctatcacgta 121681 cggaggttcc tatgtgataa gaataaacaa tctggtctcaccagacctgt ctttagtttg 121741 atctgctgac ctattactta ttatatacat tttatttctgtgtcacaacc ttcattgttt 121801 tgattttttt aataattttt ttgagacacg gtcttgctctgtctcaaaaa atattaaaaa 121861 aattttttta atcttttttt tttttttcct gacagagtcttgctctgtag cccaggctgg 121921 agtggctcaa ttaaggctca cttcagcctt gacctcctgggctcaaacaa tcctcctgtt 121981 tgattctctg tgtagctaag actacagttg ccactatacccagctacttt tttgatgctt 122041 ttagagacaa ggtctcactg tgttgctcaa gtgggtcttgaatgccttaa ctcaagtgat 122101 cttcccatct cagcctccca acatgctggg attacaagtatgagccacta aataaataat 122161 ttaaataagt cttgctatct actggatacc cctaccttaattttcttttt caagaattgt 122221 ctgaattatt tttgaatact taattcatgt gtaatttaggactgttttgt caatttgaaa 122281 ataaatttct ttaagattct ggttagaatt atattgaatatgtgaattaa tttgagcata 122341 atcaatgtct ttgaaaatta atttttgcat atctatatagttcaaatcct tcactaaagt 122401 attatagtta tgtaatttca caatatataa catcatgaaacacttttctg agacttagtc 122461 ataatcaaaa tatagttctt atttgtaata agaaagggaatttttttaat tccacatttt 122521 aatggaccag tgctggtgaa taggtgtaat tttagcccatatatattgat attgtactca 122581 gcaagcttat caaatttgtg ttatttcaaa agttttcctgttgattttta aattatctag 122641 gtggaattac tactattagt atataatttc tttaaatttccatccaaatt tctatgtaac 122701 aatatttttg tcaaagtgta ttagttaaat gacctaacgaaattgtcaat aaacaagtat 122761 ctttgtctta atcttgatta aatgaaaagc tctatgttacactaataact atgcttctgt 122821 atttgtttag tagacataca agttattaaa tgttgcttctatttaaactt ggtttgggta 122881 tttatcttga ttaggagtta aattagatta aacatttttgcaacatctgt tgagataatt 122941 ttatgtaaat taaaattatt tactttctag ttttaaattatcttcaaatt cttagaatga 123001 atcttttcat ttgtaatata ttttaagtat atattatgagactaatttac ttttacactt 123061 ttttttagca gttttgtgcc tgtatctaat tgcctaccttctcattattt gctgtcctct 123121 actgttttta ctcactttga gaataatttt ttaacttcttaaaataaatg agaataaaac 123181 atttaggttc actgaagtag tttggagaag gtaaaaattatgacattttt aaacttagta 123241 aaagtttttt ataaatccat ctgaatttga tatcttgtgtatattttgat agtccattca 123301 aaatctttga aattgttgat ttattaaagt tttttactactaatataaat ttttgttaac 123361 ctatgctttt taaaaacttg gcaatttcaa ctgagtttttaaattttgga ataaaattat 123421 ttagaacttt gattatataa agctctgcta tttagtattttctacatttc atcaattttt 123481 ctgcttctcg tatttcttga tcagtaccat cagacttttgaatttcctaa tctttaaaag 123541 attttggttg tactaccatt gtctattata ttttgattttctatttattt attcattcat 123601 ttatttattt actactttga ttttggttga catccaaaagcataattact gcccttcaga 123661 caaattctaa cttttccttt tgctgagtct atgacttgggaaatttactt aaccttgcta 123721 tcccttggat ttttgtttgt ttagtttttg agacaaggtctcacgctgtc acccggggct 123781 ggagtgcagt ggtgcgatct tggctcactg tagttttgacctcgcaggct caagtgatcc 123841 ttccacctca gcctccctag tagctgggac tacaaaggtgcaccaccata cccggctaat 123901 ttttgtattg tttgtagagg gcagtggggt ctcaccatattgcccaggct tgtctcgaac 123961 tcctgaactc aagcatccac ctgtcttgac ctcccaatgttcggggatta caggcaggtg 124021 ccacagcacc ctgtcatgcc tctgattctt taaaaaccaaagggaatgag actgttttta 124081 ggaataaata taaaataaat agcacaccta aaccacctagaacagtacca tgtactaagt 124141 aagcgcaagg tacatattag ctccttctta acttattcacccttctttaa atttttctgt 124201 tgttcatttt tcttaactga aggtgaatat gtgcctcattcatctggaaa acttttttat 124261 aaaacatgta ctaatggata tggattctta gctttagccggttttttcat tttgattgtg 124321 aaaagctttt tcgttgctga gttctaaaca tttatcttttatgtagaaat gttcatcttt 124381 ttctcagtta tagagaatag ttcaattttc aaacttttttgattgattta acattttact 124441 actttacatt ctacgtaata cttattccat ggaatctgttctttgttata gtgtacatgg 124501 tttaggtgtg taaatgtttc atgtgtattt gaatgtttgtgtccagattt ctgtcaagta 124561 cagatttcta cataaaagta ttgaattaat ttttgctaattgcattctta aaatattcca 124621 tatcattctt tcttctgctt gatatggcag tttattaagtgtgtcttaca ttcttattat 124681 ttgtggattc ttcagtttct gtttgtgagg cttctgctttatctatttac gtaatgcatt 124741 gttaggtatg tccaaattca tgattattgc atcttttccatgaatttttt ctttttcttt 124801 tttttgagac ggagtctcgc tctgtcgccc aggccggactgcggactgca gtggcgcaat 124861 ctcggctcac tgcaagctcc gcttcccggg ttcacgccattctcctgcct cagcctcccg 124921 agtagctggg actacaggcg cccgccaccg cgcccggctaattttttgta tttttagtag 124981 aaacggggtt tcaccttgtt agccaggatg gtctcgatctcctgacctca tgatccaccc 125041 gcctcggcct cccaaagtgc tgggattaca ggcgtgagccaccgcgcccg gccctttttt 125101 ttttttttga gacagagttt tgctcttgtt gcccaggctggagttcaatg gtgtgatctt 125161 ggctcagtgc cacctctgcc tcccaggttc aagtaattctccttcctcag tctcctgagt 125221 agctgggatt acaggcacac gccaccatgc acagctaatttttttgtatt tttagtagag 125281 atgggggttt caccacattg gccaggctgg tcttgaactcctgacctcag gtgatctgcc 125341 cgccttggcc tcccaaagtg ctggaattac aggcgtgagccaacatgccc agccttcgat 125401 gaattgtttt attcttacta ctctgtaatc acctgtctttaactgttact gttgtttact 125461 ttaaatgtta tgtgattatt agttataatt cagttaaatatgacaaatat aaacaagcaa 125521 ttgaatatat gcatgtggaa gagcatctat ctttcacataactaaaaaat acataggaaa 125581 gtttttgtgg cacaaattgt ccaagggaat agtgtgattctatcaccaac aaatgatagg 125641 ataatagaca ttaggcataa gtagcagtta ctgtgctcattagttagtgg tagattctac 125701 ccatttattt tctgtcactg tggttttctg ctttaggtatgcctcttgaa agcagaacat 125761 agtcgtgttt tgttttctta tttattctct gtcttttgatagatcaagca aatttattta 125821 aattcatgaa catttctgat aacattgaac ttgcttttactgtcttatta aaattttgct 125881 agttatcact ctcttttcac tctttttttg ctttgagattaaagtgataa gttcttttta 125941 ttcatttctc catctgtttt tttttttttt tttttttttgagacggagtc tcgctctgtc 126001 gcccaggctg gagtgcgatc tcagctcacg gcaagctccgcctcctgggt tcacgccatt 126061 ctcctgcctc agcctcccga gtagctggga ctacaggcgcctgccaccac gcccagctaa 126121 ttttttgtat ttttaataga gacggggttt caccgtgttagccaggatgg tctccatctc 126181 ctgacctcat gatccacccg cctcggcctc ccaaagtgctgggattacag gcgtgagaca 126241 cggagcccag cctcatttct ccatctattc taagtttctatttttatata acaaatcatt 126301 ccccaaattg caggcttaaa acagcagttt attacttttttcctcacgat tctgtgaatt 126361 gcctggactt agtggatagt ctctcattgt ggtatttcatgtagttgcca tcagataagg 126421 gatgggacca cagtcatctg aaggctcaaa tgggatgaacatctacaatg atccaactca 126481 atggctgaca tagggaggat tccctctttt tctactgattggaatagttt cagaaggaat 126541 ggtaccagct cctccttgta actctggtag aattcggctgtgattccgtc tggtcctgga 126601 ctttttttgg ttggtaggct attaattatt gcctcaatttcagagcctgt tagtggtata 126661 ctcagggatt caaattcttc ctggtttagt cttgggagggtgtatgtgtc gaggaatgta 126721 tccatttctt ctagattttc tagtttattt gcatagaggtgtttatagtt ttctctgatg 126781 gtagtttgta tttctgtgga atcagtggtg atatcccctttatcattttt tggggggtct 126841 atttgatttt tctctctttt cttctttatt agtcttgctactggtctatc aattttgttg 126901 atcttttcaa aaaaaccaac tcctggattc atggatttcttgaagggtgt tttgtgtctc 126961 tatctccttc aattctgctc tgatcttagt tgtttcttgctgtctgctag cttttgaatg 127021 tgttggagag cagtttctca taaaagcttg tggattcaactgtaaaagtg gcaatccact 127081 ctgactcccc tctccactgc agagagcttt cttctttcacttattaaact tttgctccaa 127141 cctcaccctt ttgtgtccac actccttaat tttcttggtcatgagaccat gagctcagat 127201 aacaccttag taataatatc aatgacccgg acctgttttggtaacaccag ctctggggcc 127261 aggacacagg ggcactctca ggcagaagac tgaaatctgtgcaagtggta tacaaattta 127321 tcccttcata cacaaatctc aggccagtct ctctcttggacttctaaaat tttacattca 127381 ttgtgatgag aatcaacatt tttggttcat ttcaaaggacaggctcaagt tttgccaatg 127441 ctaatatggc tcagcagaaa aatgcatgtg gcttgtattaagaaagcaca taaaggatct 127501 acattgactg ggttttcata atcacaaaaa acgaataaatttgtatgagt atctttatat 127561 gaaggcaagg aggaaaacag gtgaatggaa gcctacgtaaatgtgcttat gatttaaaac 127621 aaacttattt cctcaggaaa aatatatttt tcacctcacctgttaaagtc ttaggaaagc 127681 ttaaccttga taatctccta ataacagaac ttatgtttcttttctgaacc tcattccttc 127741 tcatattgta ataatgaaat gaataaatga atagcaagtgagtagtggtg cacactaaac 127801 tctgagtact gactgccttc tattccactt aatacctttataagaacatt gttatgtacc 127861 taagtcatgt catctataaa actaaggtaa tgataacaatcatatgtccc taatataatg 127921 gttttgatga ttaagtggaa catagtaaat gtttaaaaatgttttctatt ataaccaatt 127981 tggctaggat aggtggaaag aatagaaata tgataattaattgtgaaatg tataatatat 128041 attaataggt catcaacatc aaattaaaaa atatagatgattaccatgat agtgaaacca 128101 aagtttttag acaagtcaaa gcaaagttga agtctcagagaccattgtgt ttatttgcta 128161 actgtattat cagactgctt agttgataca gtgaatacaataattcttcc aatgtacata 128221 aactgccaga caagcaactc aatgcaattc actcactctcggagaagttt atcccctctg 128281 ccaatgttca tctttcctgg caaaccatcc ttcagtattccaggcaaact agagcaaggt 128341 gaagttgaag aagatcctac tttatattcc attcagagagcttccgggtc ttcaaaaaag 128401 atttagtcat gtgggtgtga aagagctgag tcatgaaatttggaccttat tgtaagtcca 128461 gaaggaaaaa caaggggttt gaagcacaag ggtgatatgatctaatttct acttttaaat 128521 gatcactctg attgtcgtga gggaactgat atgggatgtactgggggaag aatgttacca 128581 ggaccaggtc gaggctgtta aagacctcca ggagagagcattgtgggccg aacaacaagc 128641 ttctagtaaa gatcatgcaa gtggacacat ttgagatgtattttgaaggt ggaagaaatt 128701 agataagcta atgggtagtg cctggaggga gtgaggatttttgaaccact aggtaaacac 128761 ctgtgagtaa cagagaaaaa gttgggatgt gttatgaaagatgaatccta aaatttcata 128821 tgttgaagac tgatgcttca ggacctcaga atgtgaacatccatagtaga agggaattgt 128881 gttgttgacg gctgccaccc actctaggtg cccaagctcctgcagcagtc ctacttctcc 128941 ctttcaccca cactcactcc cagacagtgt ccctttcagctcttccatca ccccttcttt 129001 tgatttggct ggtgagagca tagctggtat ttatactttcttgatatgct agtctctttt 129061 gctgattgtt gacattggag aaggtcatgg aagggacctgagctgagcga cctttaggag 129121 catttcctgt tcttttcctg tactctgctg ccaaggagaaatctgtcttt caccaggctt 129181 gcagctttct ccgttaccca catcatcagg caatatctgtctggcacccc tctttgttcc 129241 ctttctctgg ccttcctttt tgacatgatg gttgacctgaaatcaccaag gcaggactgc 129301 tcatctccag gtctaatgga aaacagtatc tggtgaaaccaaagaaaata atcccagtaa 129361 cccactggga gctgcttggc ttaagcacct accctgtgcctaagatacaa cccatcaagc 129421 ccagctaaaa ctgatccagt ttccacaggg ccagagaagaattgccaatt aagaatgcat 129481 tcccacttca aatatgtcta tatttatttc agggaaaatatcagtctgcc tgggtccctt 129541 atgagaacat tgttgtctgt agaaaaatct aatctattaggaagccttct tgttttgtta 129601 tctgcaactc ttcagaaggg ccagaccaga ccctcgtatgcacagtacag gatccaccca 129661 tgcacatctg tctagggcct tgtctttatg attttctgtcacatggcctc tcagccatgc 129721 ctccaaccaa gctatcctca aataaacact agccgtgcacaaaaagtgaa aaattacaca 129781 aggcctacac aaggtctgaa atggaaagtg cagctggtgataccgagtag tatattttat 129841 gaattgagga acagggaatt tctaggtatc acagagcaatcagcagctga gaagaggagt 129901 accaatccag gaccctggaa ggatttttca gaaacttaatagtttattgt ccaattgtag 129961 agacaaaaat gaaaataaag atagtgagga gtaccatacaccattgctta gaagattaaa 130021 taaatgtcat ctcccacaag tcatgtgggg agaaaaaaactatataggaa gaaaattaca 130081 aacgacaagg tagtctcagc atataaaata tcaacaaatgaatgtaagaa agagaatgag 130141 aaagaattag tattagaata tcatagaaaa aatcatctttgtgaaagaaa actctgatga 130201 aactggtgac ataaatactg agtaaaaaac agaagaccaatcaaggcatt tgaggcttga 130261 acaggcatta caaatcatca agttgaatat ataagtatggccacttagtt attagtggtg 130321 aagactacgg tatttgttag aagaaataaa gtagatgatgaattctatag aatattctgg 130381 agaaagtgga ggtcttagct acatgacatc gtgtggctcactttgggaac catgtttcag 130441 tttgtttagg tctagaaatt ccttggtggt ttctgtgttcagttttgctt taataatccc 130501 taaagatatt ttttaaaata caaatttttt agctttcctgtattgtacag caccaacgct 130561 tgcctgagtg tagtgagatt cacagagttt ggaaatttcctgtagcatga ggaggctgcg 130621 gggcagcctt ctgtcatcta tgtgtaccca gcagtgtgctgtcctcacac aactcacgga 130681 taagcagaaa gcatctctat tacatgaaaa atttagctaaatatacaaat atccatgcac 130741 acacatcatg agagatggaa ataggctatc aaaagagtcagacatattga ccgtcttcac 130801 tgttagctgt aggattgctg cccaaccccc tgacacacccacacaaactc acatacacaS 130861 aaatgaaaag cacagtggaa agcagaatag cagtctttccaaacagattg aaaaacaaat 130921 gatgtctaaa gttcaatcct gacactgtgc tgattgcttggacacagttc cgtcaaaaca 130981 tttgtccaag tcatagttgt accttttaga aattagccacaaacaacatc ctccccaacc 131041 cttcctgcta ggctagagtc ccagaagaaa tgagggatacacttgacctg aagtaagcaa 131101 agcagaaccc agtctctgag gcgaggaggc ccacctggtagggagctcaa atgcgccatt 131161 gtcctgcttg tctttataaa gggagctgac acgtttctcccagcataaag ttgggagtga 131221 caccagagcc ttctgcaaga tgcttctgat tctgctgtcagtggccctgc tggccttcag 131281 ctcagctcag gacttagatg aaggtaagcc gaattgggggaagatattgt gactctgatt 131341 ggggtttacg ggcgaatgct atagaggggg aaagtggagggaagagagga ggatgagaaa 131401 acagatagga ctgaagagtt ctcatgccaa ggatcagaagacctgttgtg ccttcattcc 131461 tcatcaagac ctcataattt attgattgca caaatagaacccaataaaga atttgtaccg 131521 ggggagtgag agagtgagat ttgcatttat agagacatgggactgctggg aaggatatgg 131581 agaatgcaag acagattcag ggaagtgcag ctgtgaagatcctatactga tcccagtaga 131641 cagggatgat ggtggccttg ctggacagtg gatgagtatccatgaaggag ataaacacat 131701 gtcagagcta ttgctgaggc agagaattgg gtaaacacttgcctctgtct acatagagtt 131761 agagaatcac cagagtgaaa tattgtcatt tttttctctcctgcatgtag tattttaatg 131821 tgctgggacg ggcatttgta agattgtatc taagtggctatgtctggtgg ctcctgttga 131881 gaaagcttgc aaacataaac aacatattta cagatgaaagagggcagaag gatccccaaa 131941 tatttcattg aaatactcaa gagcccttta actaaataagcactaaggct taaggaatca 132001 tgagaggaca aacaggggcc cttctatgtt gagttcctggttgacgctca gtgtagtaac 132061 aatcctgctt tcccttacat cttcttccac ttccggtagcatcagagagt ggctgatgag 132121 atctcaaagg ggatgcacag ggtgtgatca gaggtcctttatcctcgtag aacactatga 132181 gctctgaatg attcatgcag taacttttcc catcatcctgtacttctttt ctagatgtca 132241 gccaagaaga cgttcccttg gtaatatcag gtaaatcccaataaattctc agtaaactct 132301 gtctccattt ttccctgaaa aattgatcag ttctccagtgtcttcttatc atccttgtca 132361 ggaattggct aatatcagtg ccccagagat ataaacagttttctcccaac cttgattctg 132421 gggaccatga gtaaagaaat ttgatttttc accaccctaatgtggattaa gaggagttct 132481 aattaggaag ccttgggaag gggggaggtt gggagttgagaggcaggtca gggagagagg 132541 ggccggccgt gtggtgaaga cagagagata tgaagacaggagggttttcc agcatgagct 132601 cagctcttct tgtttcaact cacacagatg gaggagactctgagcagttc atagatgagg 132661 agcgtcaggg accacctttg ggaggacagc aatctcaaccctctgctggt gatgggaacc 132721 agaatgatgg ccctcagcag ggaccacccc aacaaggaggccagcagcaa caaggtccac 132781 cacctcctca gggaaagcca caaggaccac cccaRcagggaggccatccc cctcctcctc 132841 aaggaaggcc acaaggacca ccccaacagg gaggccatccccgtcctcct cgaggaaggc 132901 cacaaggacc accccaacag ggaggccatc agcaaggtcctcccccacct cctcctggaa 132961 agccccaggg accacctccc caagggggcc gcccacaaggacctccacag gggcagtctc 133021 ctcagtaatc taggattcaa tgataggtat gattccagtttattatccat caaaggctcc 133081 aactgctaca gttctccaac ttcattgtgc cagtgaatctattgaaaagc tgttaatatt 133141 tccgtgtcct ggaacacatt tctcatgagt tttgttcaaatattctggga taaggtagca 133201 agatcttgtt tttaaacaat ctcttgaagg caattccaattttgagaatc actatcttca 133261 aattacctct cttaaatagg gttgggaatg aggacatagaatcatgttct ccctttggca 133321 ctctgtttcc tttcctcaaa ctcaaagact cccatttatttaaagtttta cctgaacact 133381 ccttgttcag gacaggctca gtcctgcctc acactagcatttcaagtcca gtattcctgc 133441 taaatggtcc ttggactttt agctgttaaa tggtatctcattttttaaaa cacatttcac 133501 atttaaagtc atacatgctt aagctaacaa aaactaatctcactgaatag acatgtacca 133561 agctaaatag caattcattt ctcctcccct ctacccttaccaaaactccc actttttact 133621 gtttggaaac tcctctttga aatatttatt gctacataactatatataat tttcccacca 133681 ctaataccac actttatatt cagttctgtg tcttacttattggaaaaatt tatttcttag 133741 ctttcacatg agtttattta gatctcttca gtgtttaYtggttagttttt ttttttacaa 133801 ttatatatga tgctattgtt tggtttttcc ataatttacttaaccaatcc ttgtcactga 133861 acattaaggc tgctttcaca ctgtccctat tacaggatatgttgcagtcc ccatctttct 133921 gaaaataacc cttaacgtat ccaacagcca caaagcatgaacaacctaac aataatcgtt 133981 tttcgtcctc atctaagcaa

Following is a genomic nucleotide sequence of a GRID2 region (SEQ ID NO:3). >4:94744101-94841750 1 tcaactcctg ggctcaagca atcctcccac ctaagactctaaaataactg ggactaccac 61 ccagtcaaat gccaccacat ttgactaatt tttataaatgttttgtagag atagggtttt 121 gatatgttgc ccaaactagt cttgaatttg gggcctcgagtgatcctccc acctcaacct 181 cccaaagcca tgggattcca ggtgtRagcc actgtacctaaccctcttct ggaaaatctt 241 aaRgccttag attgcattcc tgtctttata ggtcatatttctcattattc tcttcttttt 301 gaccttttct ttgcaacttg agagttttaa aagttcacttattgtgtaac ttattcatta 361 gtcctgatat atcatttgca ttctttacta ctcccaactgttatttttat tctgcagtag 421 aagtttgtct ttattctttt ttgttttctt ttctcatattatctcatctt ttatatcatt 481 accttccttt cagcctattc tctttgttgg ttctctactgatgtttcacc tagattttca 541 ttgtttaaag gcattcatca gattatattg caacatttttattgaatgca gccagaattt 601 atgtgaggta tgtactacct ctgagacttg agggtgatattctgatttgt ttagggtgca 661 atgagttttt gtgtttgttt atgtttctat aaaccatccaggcccaatct ttatttttgt 721 caatagttaa agaaagtgaa gcatcttttg cctcctctacaatccattta gttaagttca 781 gtttttcttt ctcagaccta gagttaaaga tcaaatagatatgttcagca tttaatcaaa 841 tttaccatgc atagtaggta ttccatgtat aggtttttgacagagattta ctaaagttat 901 ttggtctaat tatgacagag catttattcc agacactgtcttgtttatac tacttatcac 961 tcagtgacta aatcttccaa aacaaaaatt attgtcatctcaccctctct acttccataa 1021 ccttacggtg gtttctgctt tgagtagtag tctctggatatttgtgaaag agtgtaactg 1081 agaaggcagc aggtagtgaa taagagaaca aagtctctggatactaataa agatagttct 1141 catatagttc aggatagctt gctgcttact tggccataaagctcttacag cctaaagtta 1201 ggcataatca aaggaaatat tgttgcggga agtcagggaccccaaatgga gggactggct 1261 gaagccatgg cagaagaaca tggattgtga agatttcatggacatttatt agttccccaa 1321 attaatactt ttataatttc ttatgcctgt ctttactgcaatctctaaac ataaattgta 1381 aagatttcat ggacactaac cacttcccca atcaatacccttgtgatttc ctatgcctgt 1441 ctttacttta atctcttaat cctgtcagct gaggaggatgtatattgcct caggaccctg 1501 taataattgc attaactgca caaactgtac agcatgtgtgtttgagcaat atgaaatgtg 1561 ggcaccttaa aaaaagaaca ggataacagc aattgttcagggaataagag agataacctt 1621 aaactctgac tgcctgtgag ccgggtggaa cagagccatatttctcttct ttgaaaagca 1681 aatgggagaa ataatgctga attctttttc tcagcatggaacatccctga gaaagagaat 1741 gcgcacctgg ggataggtct ctaaactggc acccctgggcatggtcatct cttatggtcg 1801 agactgcaga ggtgagatag actccagtct cctatagcactcccaggctt attaggaaga 1861 ggaaattccc gcctaataaa ttttggtcac actggttgatctctcaaaac cctgtctcct 1921 gataagatgt tgtcaatgac aatggtgcct gaaacttcattagcaatttt aatttcacct 1981 gggtcctgtg gtcttgtgat cttgccctgc ctccgcttgccttgtgatag tctattacct 2041 tgtaaagtac ttgatgtctc tgacccacac ctattcacacactccctccc cttttgaaaa 2101 tccctaataa aaacttactg gtttttgtgg cttgtggggcatcatagaac ctaccgacat 2161 gtgatgtcta ccccggacgc ccggctttaa aatttctctcttttgtactc tgtcccttta 2221 tttctcaagc tggccgacgc ttaaggaaaa tagaaaagaacctacatgaa tatcggggca 2281 attccccgat aaaatatatt atatagggag actcaaagcatatttctacc ttccctgtac 2341 taatttgaaa aagaatatat atggttgata aatatatatatatatttaga ataagtatta 2401 agccccaatg ttatgcatac tcaaaggaat tatacaacataaagagaatc aaagtatatt 2461 tctactYtcc ttggactaat tcgtcctgtt ctggttgtaagtatgggata tgaaatacat 2521 agatgaaatg tatgcttttg aatctatccc caatctcaatttagttgttt caaattattt 2581 gagaatttct catttatctt tctctaactt gaattatggagcctgtggct ttattttttt 2641 ttttttaagt tttttttttt ttggtcagtt gttgaaatttttaaacagat atttggagga 2701 ggtgtatcag agccagctag ttttaagtct cagactggtcactcttaaaa taatcccacc 2761 cagtcatgcc atcatcaaat ttagttgcct ctctccttggcttaaattta atacattttt 2821 tccccaatct attggactct ttagaaatgt tttaaacaaaaatgactaat ttatttttta 2881 aatgtaatta attttgttga ttgaataaga atgtttaaaaatgaaacatt attgtgaaaa 2941 ttaccacatc cttggtttaa aatagcctga gaaaggaaagcacagccata agaaataaat 3001 atacgaatag gttaatacag aggaaattgt gccagacaaagctggttgct tttttgatag 3061 ctttacagcc ttagtaaatg aacagagtgg agtagacataatgcatcttg acttctagca 3121 aagtactgga gacaatgtcg tgtgaaattt tgattgcaaaattaatttga gttggcttga 3181 aaaacactgt taaaggaaat ataagctggt tgaaaagacatacagagaag agtgttgatt 3241 aatgtcagtg aactaatgag ttgagtgata tctccagttaaatgtcacga ttgtgagtgt 3301 tggattgaat tatttttgtt atctgttgaa aaaataaataacatagggtt gattaaatta 3361 gagcagaaaa tttatcagga gacaccagtg attacagaaaatctatataa agacatgaat 3421 tcatttcaga gaaaaaccag tgaaacaata taattttacttaaagagtcc atttttttaa 3481 tcacaaaatg gaattctatg aagtatcaat gcttaaagaccttcaaacta cagaatttct 3541 caaaactaaY tggaaaacaa aattaactat ctcagtaaggctagaacatc attttaatcc 3601 agttagtagc tacattttat cccaaccaat aaagtgacagttaattctta cctagaggat 3661 gtggttaaat gtataattat tatttaccag aaataaaaaaagtatctaga aaatcagtaa 3721 cctctagaaa ctcttgggca caggagaggg agaaaactatggaaggggaa gtaattatgt 3781 tcttaactat gttacattct taagttacat tcttaactatggggatatag taagactcta 3841 aataactaaa gttttttttt attatacttt aagttttagggtacatgtgc acaacatgca 3901 ggtttgttac atatgtatac atgtgctatg ttggtgtactgcacccatta actcatcatt 3961 tacattagat atatgtccta atgctatctc tccccactcccacctctccc cacaacaggc 4021 cccggtgtgt gatgttcccc ttcctgtgtc catgttatttttgaaatatc cagattaaat 4081 gtagttaagt aaaacaataa actttttcaa tgtaggttttttaatgctaa tgaaaagcta 4141 gtcagcatgg ggtaaattag attaaattag attaggtaaattggaataag aatcaaatga 4201 tcacaatcat aaaatgggat tagtcttact ataagtcacacctgcaaaag gaagaagata 4261 acctggaaaa atttaaagag tcttccactt tacgaagtcgtagtgagttg gcaagtttta 4321 ataatttaaa agaaaacgtt gatttcatct aaaaagagagagagaaagtt ttagttattg 4381 attcaacatg tatttattga ataacttaaa tgtgcactatttttggtttt gtgagcagtc 4441 ccaaacgatt aaaacagagt gacttgttcc ctgactatcttaaattatcg caaactgatt 4501 ggcttaaaac aacggaaatt tattctctca cagttttggaggccaaaagt tcacaagcaa 4561 ggtatcagca gggctgcact ccccctaaag gctttgggggagaatgattt tgagcgtttc 4621 agcttctgtt agctgtcagc attctttgat ttgtggcaacatcactccaa tctctgcctc 4681 catggccatg ttgcctcctc ttctgtctga caaatctccctctaccatag tcttaaacaa 4741 acagttgtca ttgggtttag tgcatattca tgtaatctagaacactctcc cctctcaata 4801 cttaacataa ttatatctgc aaagatcctt tttccaaataaagcaacgtt tgtttccaaa 4861 ttttaggatt taatatctct gggtgaccat gatttaacctactacaaaag cattttctaa 4921 caagcagctt aaaataggtt tcttttttct ttgagacagggtcttgctct gttgtcaagg 4981 ctggagtgca gtgSacagtc atggctcact gcagcatcacactcccagtt tcaagcgatc 5041 tttccacctc agcctcccaa gtagtttaga ggacaggtgcatgccaccac acacagctaa 5101 tatttcattt ttttgtagag acagggtctc tctgtgttgaccaggctggt ctaaaactcc 5161 tgagatMtag caatcctcct gccttggcct ccYaaactgctgggattaca ggtataagcc 5221 actgtgccag gctcaaaatt aggtttctat tgctgcattaatatatacac agRgccatct 5281 ctttctcttc taattatttt acaaatataa agagcatctgtaacacaatg aagctgtttt 5341 ccactttagg aaaagacagt tttgaaaatg tgattaattatttgttgcag gttaggcttc 5401 atacagtcat attcccagtg cacattgtcc tcaagatctccccagtttgg cactgaagcc 5461 ttattgactc tgtctctgaa agaggcctta aatgatcccccttccttcta atcctcactg 5521 aaattccctt aggttaagcc caatttatcc aagggctatggtcttctact tgatgttctg 5581 tttttaatcc ttgatgctat ccttctcctt ccatacaaacagtagataca catattttca 5641 aaacacagac tttcttgtgt tctctactta aaaatctgtctagctttcag atacataaag 5701 gaaaagcaac ctggttttaa gagcatgcaa tgYttcttcttaaatttccg atccctgtcc 5761 ttgtctgcag atttatcact taacattcct ctccttgcttcataatacta cacacacaga 5821 catacacaca cacaaacaca cacaaacaca cacacacaaacacacacaaa cacgcacaca 5881 caggcacaca cacacacgca cacacaaaca cgcacacagagacacacaca cacacgcaca 5941 cacacacaca cgcacacaca gttccttgat attgcagtcacacagaattg ttcactgttt 6001 cttgaaaata atcataaact gcatttgctc atgttattttttgagatcag agtgttcttc 6061 cctctttttt ctgctttaag gttttctctt tttttgcacagctattaaaa cttggccaac 6121 tattatattt tctctatagt tttccctgat ccttacacatcttcatagct ccttacatgt 6181 aactctatta ctgtgtgcat catatgcgat cgtgtttattttccctgtgt gatttgtttt 6241 ttagactatg accaccttga agacagaact gggtcttatctatctttaca tctcctagtt 6301 gtttgcatgg ttcttgacac atagcaaatg ctcataaacttctgatgaac taaattactg 6361 taaattaaag tcattgatac atttaaatgg cacgatttacctatatctgt tttataatca 6421 tagttaacaa attaagcaaa atacttctag gaccaggaactcaatatgga gacactccat 6481 tgtaagagag aaagatgcat tgaatcgttt attcagaaagaacttattat gtcactgtag 6541 gcactgcaca aaggctaaaa atcccaaaat agaattgttaaaaaattagg gtttaagatt 6601 aaaggcaaag gaaatcatat ttgttgagac tattagggttgcttgaagta aactaatgcc 6661 aagtttaagt aaaaagattc ctgctatatg atataattgttaagcctaag agaccagcag 6721 gcaccaggta cagttacatt cagatgctca agcaatgccattagggaact gcccatgttt 6781 agccattact gtgttctgtt attccttttt tttttgttttgttttccctc tcaggcagat 6841 tctactcatg tggtaacaag actattattc tagatactgtactcatattt aacagtctca 6901 gggaaagagt gaagcctttt actcaatcgt tccagaaaatatctaatgga tgaagggaag 6961 gctctaactg gccttcgttg gaccacttac atttctttgaaccaattact ctggtcagaa 7021 gatgaacatg tctgatcttt caggattaga ttcacatgcaaaataatgtt acaattcaaa 7081 ctaggaaaaa taaattatat atttttaaga gactggatgaaaatactcca tacatcacat 7141 gtgcttttct ttttctgtat attggtatat acataatcactatttctttt tttttttttt 7201 tttttttttg gagatggagt cttgctgtgt tgcccaggctggagcgcagt ggcatgatct 7261 tggctcactg caacttccgc ctccaaggtt caagtgattcttctgcctca gcctcctgag 7321 tagctgggat tacaggcacc caccaccaca cccggctaatttttgtattc ttagtagaga 7381 cggggtttta ccatgttggc caggctggtc tcaaactcttgacctcatga tctgccagcc 7441 tcggcctccc aaagtgctgg gattacaggc gtgagccactgcccctgacc caccactatt 7501 tcttttgtaa ttaaaaagta catatacact ttatattttgatgtagatgt aagaatagcc 7561 tctgcttcaa agacctcatt ataatagtat taaagacacacaagtaaaca aaggtaacaa 7621 taaaatagta atacatatca aatattaagt atctcaggaataattattcc ttctaaggaa 7681 tggcaaagaa atttttcaca ccacaaagtt aattttgaattgggtcttca aggaacataa 7741 caatttgaaa agacagagag gactttccag gaaaatgtaactacatatgc acaaatgaca 7801 gtagggatta tagtaaKWac acttttatta ttattctacctcttcactct attcaatttc 7861 cctgactact acaatgtccc catgttggtt tctcctttcttgtattccaa gatattgtgt 7921 cctgcttttg cttattattc tctacccctt aagcttctcattgcttcctc cttcttttct 7981 cttttttctg ggcctccctc tttgttttta cgatccctgggaggactcat ttaattgact 8041 cctaaatggt ttctgtgtct ctagtgtctt gtagacattagaggcacaga aaccattttt 8101 cactgtctat tttccactcg gcatccaaac taattttccataaacattgc ttccatcatg 8161 gatatgtgct ttaatagcat tggaatcaga cagatcttaagcacaataca atagtatata 8221 taccagatac cagttatatg actgtgagaa gatagtaacttctgagagtc tcactactaa 8281 tgcccaactt gtaagataat tatgaaaata tgtacaacatatgtatcact ctcagcagtg 8341 ctagccacta gatagatgtg caatatataa gtagataatacttttgtggg tatctatatt 8401 actcctttgc ccataaccaa cagtgaacca tcatgttcctttttaagctt ggtggtggat 8461 acaggtgttc actttaaaag tctcagtctc agatttcaagtcttcaaccc atctcctaag 8521 ctgtcagagg cctttacaat ctttacctac ctcctttattcagtcttatt tcctatcatt 8581 tccccacagg aactatttgc tggcccttta aKttctcccttacctcagaa cttgctaata 8641 tttatcactc ttaaatatat gtttttgctt gtgctgttcctcaaactaga atttttctgc 8701 catcttttgt tatcctctct cttcttttag tttaaataccttctgttctc taagtttttt 8761 ttttccagga caacctagaa aaaaatatct ctttagtttctacagtggca gaaggtaatg 8821 tgatttacca atttaattac ttcatattac aatatagtgaccatttatat cagtgtatag 8881 tacaaatatg tatactgtgg tcttactact gattatcttattcttattta ttgcctctcc 8941 aacaagatgt tatcacccat agacaagaat catattttatatatctttat aacccaaagt 9001 gcctttttaa aattaatgta tcatgtttgt tttcctggaacaggaactga aatagagagt 9061 atgaattcat ttgtaactga atctttgctg taatagttatccccagcatc cttgctaata 9121 cagagaatga agccttaata cctaaagcag ccactgtRtgtgattaatta acttctctcc 9181 tatgcatctt gacaggtagt ggctatgtgc cattcttgggagaactggga aaatggtcac 9241 tgaaatcatt tttgttgttt ctagttccaa tgagaaatgccagcYgggaa aggctgctac 9301 agtatgtagc aaggtgcttt tgctgaaata ataaaagtatttgcaaggaa atacttgaaa 9361 taagtgctaa atttccaaag attgatctac actacagaaggcttattagg gtatattgat 9421 actagtatgt gttgtcttaa gtggtacatt gtagtttccatgccaattct tggtttaaaa 9481 cgcagcatgc atctgttata tgccactttc cacttaaatgcctaagacag gtagattgca 9541 ctgtcaagtt aggtcattat aactccaaca gatgttgtcatgtagatgaa gaaaattcat 9601 tttaaatgat atcatttata aaatgtgttt ttctttgtacctaaaaaagg caataatatg 9661 tcatattagc tcttcagatt caggttgcaa tcagtatggcatgttttgat taaacacttc 9721 acaatttcaa aatcagtgca ggataatgat ggagcacatgtgaggaaaca gcaatagcag 9781 gggtgtttac agtatcccag ttgtttcaag cttaataacacttcatgtta tcaatcagtt 9841 acaaaggaat aattggtgga taggctgagg aaatggtacagtaagaaatt gtacttattc 9901 taaactatag ggatggaagt gcagccaggt caatacttatttggttagta tattgtaact 9961 aatcatttga gttttgccag ctggtatctt atttttgcttatttgtttaa ccgcttttaa 10021 ataagtatat aattttaaca atttaagtca gttgaaatcccccccataga gctctcctcc 10061 aaatgcatcc ctagctacat tcttctttct ggagattaaccagaaattgg tggtttcatc 10141 ctgtttatat gaatacattc ctagaagata aaatcatattgtagatattt gtgacaaaat 10201 tttcctcaca actatatgaa agagaacgaa ggaaggtgtgtggttaggag ttagctgtca 10261 ttaaaagtgt gtaaattttg cacgttccct acatttattttccagtaaat tttactagtt 10321 tctttctaat cagttgggac taaagtcaat cgacctgtctctgcttcttt caatttcatt 10381 tcccaaaaga attttagtgc ttctctatta ttcttaactctgccttgccc caatacatta 10441 atcaatgctt tgggtaacaa cattagattt gtacagggaaggaccaggga atggcattcc 10501 aaagcaacac atggaaaggg caagagctga atttcataaaccgtaaccta atccatcact 10561 gcagctttcc gttgcctcta cccagactgc agccaaacatactctaagct gccactttag 10621 agtatcttta ccctccatgg gagtttttct ttttttcccttttttttttt ttttttgagR 10681 cagaatctca ctctatcgcc caggctgaag tgcagtacaggatcttgtct cgctgcaagc 10741 tctgcctccc gggttcaagt gattctcctg cctcagcctcccgagtagct gggattacag 10801 gcactcacca ccacgcccgg ctaatttttg tatttttagtagagatgggg tttcaccatg 10861 ttggccaggc tggtcttgaa ctcctgacct cataatctgcccacttcatt ttcccaaagt 10921 gctgggatta caggtgtgag ccaccgcgcc tggccaggagtttttcactg tgatgttgtg 10981 taggtctctg gaatgtatgt ttccctctgc agtttccccagtgttgattt taagggagga 11041 agccagagca cttgctattt caccatcaca cttgtttgaagtgtgagtta ccatgcaatg 11101 tttaaaacat gagttacacc tttttggagg atgctgtagaataccgattt attttttgtg 11161 tgaaaacaaa ctctacctca ggttaatcaa ataattactgaagaaaaggt ttttctttat 11221 tcaaatattt cagcacatga atgaagaaga aatggtaaaagtaaaacatg aacatttggc 11281 catcactaat tatattagtt tgtagaggct actgtaacaaaattccacag accaagtggc 11341 tgaaacgaca aaaatttgac tgggagttca aaagcaaggtgccagtggat tgggttttcc 11401 ctgaggcctc tctcttcagc ttgcagatgg ccaacttttccctgtgtcct aacatggtct 11461 tctttgcacc cgatggctct ctttatggtc taatctcctcttcctgtaac tacaacagtc 11521 tgattagttt tgggcccacc catatgatct cacttaaccataattatctt ctttaaagac 11581 cctatcttca aatgcattgt gaggtactgg gggttagggcgtcaacacct aaatttgggg 11641 aaacacaatt cagctcatta aatgaatgga agtcatacctgaaatcaatg acggatcgta 11701 tgactacaaa aaaaggagac aaacattata cacctgctgtttaaagtata caacacaaaa 11761 ttattgacaa atattaagct gaattaggtc aatcttctagatttaccaat ttgcaaataa 11821 tgcaatccac agagcaagat gtggatatgg gggattcagacagcaYaatc tagctcggga 11881 aactacagaa aaaaaaaatg acccattttg tgacatataattgaaggaac aaatgaagat 11941 agatgaatgg agaacccRta aataaagcaa ttcaagatacacatgaactc ataaaacata 12001 ggtcctaatt tgagtaaaga aagaaaattt taatactgactggWtatttg atgacactga 12061 gaaactattc taactgtgca gctatgaaaa ggaatgagatcatgtccttt tcagggacgt 12121 ggatgaagct ggaagccatt atcctcagca aactaacacagaaacagaaa accaaatgcc 12181 acatgttctc acttataagt gggagctgaa caacgagaacacatggacac agaaagggga 12241 acaacacaca ctggggcctt ccccggggbg gagtgcagagagggagagca ttaggaaaaa 12301 taactaatgc atactgggct taatacctgg gtgatgggttgataagtgca gcaaaacacc 12361 tttgcacaca tttacctttg taacaaacct gcacatcctgcacatgtacc tgcacatgaa 12421 ctaaaaataa aaataaatta ttctaaatct tgggaagtatgataatggtg tcatgattat 12481 gttttaaagg acttcttaac ttttagagat atataccagaatagaaataa tattgtataa 12541 tgtctgccat ttgtttcaaa tcaaatctga ccaaagtaggggcaagaaag agtaggcgca 12601 taaataaata actcattgat aatgagttga taattgtttcagtaggatga tgaatatttg 12661 agtttataat tttattctat ttatatattt gaaaatttttatactgaaaa gttgaaaaag 12721 ctttatattg gtattttaaa ctttgtaaat gtKcactaaaagaagatatg tctttttagt 12781 tgttttgcct gagttatgat gagctttgat gagtcaatcaggctgcctct tgagttttcc 12841 ttgtaaggtt aattgggtgc actggagagc tagctgaggttaggagagca ggctctggtt 12901 gaaggacagg gtttcggctt ggcagactaa ctaccttctctgattattaa actttgatgc 12961 cacccacatt agcacagtat tctagtcacc tgtgataacctgtccagaag ttgctctgat 13021 cgtgttcagc tcaataagtt tagacttcac acaatagtgcttgtcatttt ttaccctctt 13081 gtaccaaata tatttcaaaa tatgtccaat ccagctgttaatttgactga ataaaatatt 13141 ataataacac ttctgaggat tgagatgatg tttgaagtttttcaagagtc ttttccctct 13201 ggattagaga acatctctgc cagaatcaaa atgagtctcaaaattgacag tttttacttt 13261 acttgctaca gtaacctggc attttaaaaa gcattctggaaaaaaaaaat gaatcttctg 13321 attgtgtaat aaaagaataa gaatagaaac taagataaaatgattctaca tgtatatctt 13381 gacttatgtc tatattttat aatttcaact tttacgtcaaaaaattgtaa aatgtcacta 13441 attttataag gtttaattta acagatcaaa gctcataggactttgttgaa aacaatgaat 13501 gcaaatcctc agtacagttc caggtgtgtc atgagccctccaaaaatgat agttacaatg 13561 actgtttcaa aaaatttata ccttcaacaa aactttaatcctatttttat atgcctgtct 13621 gctctgaaaa taatttaata tgtttaaatt cctagaataaatcttacttt aaccaaatta 13681 ataattctta aacttaaatt tagatttcaa tgaatccccaaaatggtttc cacataaagt 13741 aatgcagcta atatcagcac tgctaacaga ttacacagaaaggtctatta taatgattga 13801 gtaaatattc ctgggaaatc aaccttgtta aatctatatcctataaggaa aggtgcatat 13861 ctgttttttc tagtaaagat tccgtaattg ttattacaagttaatcagct tctcccaggt 13921 tcatttactc tggattttat taatttatta caggaaattattgggtaagt aaaccacaat 13981 tataataaaa tctcatacat tttattatat ggttcacaaactttataaac acctgtgatg 14041 ggattctgct attgatattt aagtggatat ttattaaggaactagagata cttctcagta 14101 agattagctt agagaacggt cctgtttttg atctcttaagattccaaatt tttattccaa 14161 attttctgat attgcccaag tgtcttttct gaatctatgtctttattatt tggcttaaaa 14221 cctttgtgca tctggtgctc tattctgctg tgactttggagaaaggttac agtttgtttt 14281 tgctgggagg aataacttct tgaatgatgt cccaactgaaaattattttt ttggttggat 14341 agaacatccc tgtacaaaac ttcctctatt atcccttggataattcctgc tgtatataga 14401 ggtccaagat tgattaatga ttttatcttc agtaacctgtgatctaaatt accattggca 14461 agacaagagt gatacctgtt tttttattct ctacattaataaaacatgcc ttaagaaatc 14521 tatatacttc atatagagga tatgatttgc cactaagcaaactctatgaa aaagaattgt 14581 gttttcatag taaggcttta ttataaacca aatatgaccaactttcagct aaactgtatt 14641 atctaatcaa gtttattccg tcactttact tgctattatcaataagacaa gtaatatatt 14701 taaatataga ataagtaaca agcaacatgt taactgaattaatatattgg taaaatatag 14761 attttaaaag ttttttgaaa atatttgagg agggttgcacttttgctccc tctgatttta 14821 ctacgtatgt ctctgactgt atgtaatcac acctagcttatgctgtccct tccattctct 14881 acacttgact ttagaaagtc aaacgtagac tggagcaaaaaggggagaga tcttttccta 14941 ccatttagca gattaaaagg tagattggaa atgcatctcagtatctcagc cataaatgtc 15001 taagggataa taatcccgct acatatttat taaactaattacaacatatt gcgcaaaata 15061 aaatactttg aatcagaatg acttatgaat ggtcgattgcatacaaaagt gctggagata 15121 tggtgataaa aagatgattc agccatcaca aagctcaaaattctactggg agaaacaggt 15181 aaagtgactg aagcccagga taatatttca aataataagtacagtgagat acttatttga 15241 gagatgcaaa ctcaacaaca gtttggtaag tactttctctattctaattt gaatacattc 15301 tagtcaattg agatacacaa agcatcaatt ctccccattggatttggatc ccataaaact 15361 acccatttcc ccaaatagat agtgttttaa cttaacagacctcataaaaa gcttgacact 15421 aaaatgagaa tttataaatt gtgatctgta gtaagttaaatggtgcccaa ccctgcgaaa 15481 aacaaaaagt atgtccatga actaatccct ggaacatgtaaatgtgatca aatagggaaa 15541 agatatcttt gcagatataa ttgtaaagaa tttcaagatgagatYgttat ggaatatctg 15601 agtgggctcc aaatctaatg gcaagtatct ttattagagaaagacatacg gggatttaaa 15661 acagaaaagg aagaggagac agccatgtga aaatggagacagagattgaa gttatgcagc 15721 cacaggtcaa agaatgcttg gaaccaagag aagctggaaaagacaatgaa agattttccc 15781 ctagagcctt tggatagagc cctgcaaata ctttgatttcatatctgtcc tctggaactt 15841 ggagagaata attttttgat gtttaagcag atgactttggggcagtttgt tatggcagcc 15901 attgaaagca agtcgtgatc tagtttgcct tattttccaaagcatagata attttcaatt 15961 tcaattagga aaattgaaat tccacaaaat gcaagtgcatacagaaatgt ctatctgagt 16021 tgcagttggg aaacattttg catttaaaaa ggaaaagttttaagatataa aagaaagtct 16081 tgagaaaaca agagaatatc cctcatatat gtacaacttcttactaatag aagccttaga 16141 tgtctgatca atttcagagg caaaggctac cattgactgttcattgacca ttgacaagcc 16201 atgtgtttca atgatgaaat accacataac aaaatactctaaagcatatt gtcttaaaac 16261 aactgtttta ttactaaggt tcctgcggat ctgatattcaggcaggattc agtggggata 16321 gcttacctct gttctacatg gcgctcactg ggatagttcaattagggtaa gaggattgaa 16381 gatggtatca tttgcatgtc tggggctttg gtcctggctgttggctgagg tgctttagtt 16441 acccttttct ctccacatag cctctaatta ctcaatattctaccccagcc tttaaaaaaa 16501 attttattgt ggtaaaatac acatataaaa tataccatcttaaacatttt aagtgtacag 16561 ttcaatgata ataagtacat ttatattcct ttgcatccatcatcacccat ctattttcaa 16621 aactcctttc atcatgcaaa actgaaactc tatacttagtaaacaatgat tccccatttc 16681 cctctctgtc cagcccgtgg caactgctat tctttctgtttctatgattt tgaccatttc 16741 aagaaactta tgttaagtgg aatcataagg tatttgttattttgtgactg attcatttca 16801 cttagcataa tgtcctcaag gttcatccat gttgtagtatttctatcctt tgtaaggctg 16861 actgataagg agagacttac ttctgtcatt taaaaacttgttttctatat ggcttatagc 16921 atttttgtcc cttgcttcct gcgtgactgt cttcttttgtgtttagttga ttattgtaat 16981 gaaatgttta tgtatctatg tcattgactt ttgtatattttctatagcta ttacatttaa 17041 catcctacag ttataacact cttatttaga tttatgccagtttaactcca ataatacaca 17101 aaactctctc ttttacattt ccatacccac ttcttttggttgctgatgtc ctaaaactac 17161 acctttacac attgcaggtc caaaaacaaa aactaataatttttaaatat atcagtttct 17221 tgaattaaac cattgttaca ataatagtag cttttataattgtccatata cttaccttta 17341 atcttcagaa ctcacattaa cgtttcttcc agacaagtctagtgttaaca aactcctctc 17401 aacttttgta tacctagaaa tgccttaatg tctcccttagtttgaaggaa atttttgcca 17461 gatataggat tcttgcttga caggtttatt ttctcttagcactttgtata tattggccca 17521 tttctttatg gtttccaaaa tttctgataa gatatatgcttataatttta tcaaaaatcc 17581 cttgtatgtg attaatcact tctcttttga tgtttttgatattctctttt ttgtcttcat 17641 catttcaaag gttgataatg tggatctctg agctcattgtatttagagtt tactgagctt 17701 cttaaatgtt tatattcata ttctttgtta tatttgggagttttggccat tatggcttca 17761 aatatcctct cttcttttct ctgtcttctt atggactccataatgcatct gtttgtccac 17821 ttgatggtgt accaatggtt ccttattctc tgttcacttttctttaatct tttttctttc 17881 tgttccttgg attgataatt cccatggtct tctctttacatttgctgagt ctttcttctg 17941 gctgcttaaa tctacctttg aaaatctctg gcaaatttttttcatttcac ttgtactttt 18001 tagctacaaa atttctttct acttttaagt tttctatctctttatggacg tttataattt 18061 attcatgtat tatttcttga tttcctctac ttttcttttagttctttgag catctttatt 18121 acagttgttt tattacagtt gtccagtagg tcccccatcatgtctttttc agtgacagtt 18181 tctgttggct tattttattt tatttttaaa tgagccattctttcctgttt ctttggatgc 18241 cttataattt ttttttgttg aaaattagac atgtgactgtagtaatgtag taactccggc 18301 aatcagattg tacttatttc tcctaggttt tcctaggagaaatttcctag gttttcccct 18361 tttgttctaa atgttgtaga ctagactgtc tctgtgcaaaggatcaatct gagatgtacg 18421 taaatttaag gtcttctcaa gtctcttttg agcctgcacctttccctggg catacacagc 18481 aactttctaa tttcccgtgt gtatgtggtt gcttttaaatgtcttagtat ttaatgtcta 18541 cctccaaaaa gaggaaaaga gaaaaatgaa ggtggcaagcaggcactggc catttaaatc 18601 ccctggaaat tacttcagcc agaggggtag gggtgtgcaacaatggaggg gaggtgcaac 18661 aacagtgact acctagccct tcgtctacaa gtccgtgattagaagcagca gtaagcaatc 18721 agagcacaga tctccaatat ttggattata acattccttttgcccatgct ggcttttgtg 18781 aactgcatgt aagttgctcc tgaaacatat tcatgNctgcctgccattgg actaagggtc 18841 aggataggta gctgctactg ttttaagagc ttaaattgatcaaaagtaac cataattttc 18901 cttataagct ttccactgga agtttcaagc cttcaatagactccagaact ccagaatatt 18961 tatatcagat agattctgcc agtgcagttg ttgcctacatggggagacag atttctggtg 19021 ctttctattt tgccagcttc ccaaaatctt cctttctaatcttgtttctc tccttgaaag 19081 ctggggagaa ccagctgtaa ttctaatttt tgttgttagtggactatggc tgtgagaaat 19141 gtcctctgga tggggatgcc actaccccct acacagctcacatctcactc acaactttgg 19201 ttttcccctg ctctggacca gccttttttt atttctttatttctttttca cttgattggc 19261 ttccaaaaat gagaagagaa aacctgtgag atctcttaagattcatgcca ccaaatcata 19321 tgtcatcact tctgccacat tttactggag aaaactcacagagcaaactc agattcaaga 19381 aaaggggctg tcttagacct tttgagcttc tataataaaataccttagtt tgggtaattc 19441 ataaatagta gtaatttatt tctcagagtt ctagaggctgagaagtcaag gcatcagcag 19501 attcaatacc tggtgaaggc ttgctctctg cttcgtagacggcacatctt gcagaagggg 19561 tgaacagtat cctcacatgg ccaaagggga aggcaacttccttcagttca ttttataagg 19621 gagctaatcc tattcatgag ggaagaaccc tcgtgaattaatcaccttct taaatcttta 19681 atacaattga gttccaacat atgaattttg gagcgcaacaatattaggga agtggactcc 19741 atctcttgaa agaagaatgg tatgtgtgca cagggataggaggaattatc tgtggccatc 19801 ttgcaggcag actaccacac agtgctctgc tttttcttatcattttcttt gatgacatct 19861 gatcctttct cacaaatatc tcctttgtaa aacactcagcacttattatt tatatcagtc 19921 atttgggaat tagtcatatg ctgcctagtg gaatttgttgtcattaaaaa ttttttcctg 19981 ttttgtcatt tcaaaaacaa tcacaaactc ttggaggttaaagatgatgt tctatatttc 20041 cttgtacccc gtaaaaccag catagtaggt atttaataaataactgtctt cttaattata 20101 aattttaagt ggtgggagaa tgatgtattt acactaatgcacggtggttg tattattatc 20161 cactagattg tttcttgctg catatcatgc actgcattatttcttgctgc atatcaatca 20221 cttagtactg acctcctttt taaaatacgc ctgactttctcatgcttatc ttacatatat 20281 ggttatagct aagagttgtg aatggacaca gatatgggaagcatatttag gaggattaac 20341 aaaagagtaa ttagaagaac acataaatcc acaaggaaaaaatgactctt tttgaagtat 20401 gctagcattt tcaacatgcc tgttgagtta atgatttatgggagtttcaa ttgtttaaat 20461 gtttggaata ctctgcaata aaataaaaat tctcttcaaagttgataaaa atactcagag 20521 gcatacttcc tcatctgatg attcatagtt accaatgaaacccaagaact ccatgcacat 20581 tttcagcaag agcaaggaat ctgatagcaa gagagctggaaaggaagaat catacagtga 20641 cctagacaag tgtcatgtcg gtcttagcat cagataaactgattgcaatg gttgattgta 20701 ctatctctag cgagagtaat ttagaaaacc tgaataagaaaccctctctt gttcatcata 20761 gggatgactc ttggtgtgaa cccaatcata ttaaacaattgtagaatcac caagctagac 20821 attttatgag actaactgat ttattcctca tcttgaaataaaacctgcat gccacaaaac 20881 tcctctatgc tgctgagact tagttctttc tttgagaaaaataagagcca tttattcatg 20941 caataaattt tcattgtgtg tgaattgtat actaggcctgttccatgtgc ttgccatacg 21001 tcagtgaata gaagaggcaa agatctttgc ctatggaacttgcagtctag taggggacat 21061 aagttatttc gtaagcttcc cctttataaa tatcattaatccttactaat aataatactt 21121 acatcaatgt ttatatattt tacattggtt ttctgataatataaaaaaaa attcacaaaa 21181 taaacccaac cactaataag ctgtgagttt tgagtgtagaattcttttca ttaacaaacc 21241 atttcatttt cagatcgaga aactaaggtt tttaaaaaactgattttact gaaaataaaa 21301 tctaaccacc catcctaatg ttctttactt ctttatctgtctttcaaaaa tatcttcctt 21361 tctctatgtt gagttagata tgtattcaca aaatctaccatactgtccat atatgttaga 21421 tataaatttt tgagctcatg tgaaagaaac caagtttgaatagttgaagc aaaacagaag 21481 ggtttattat taaaaaaaaa atgtgttttc tcaaggaccccaaaacacaa agctgaactt 21541 gagaagtgag cgctcaaaac tattaaggct gtctgcatgacccttgctct aattcctctc 21601 tgaaatagtg tctcattgat tgaggtaaaa aatgacagcagtttctagag ctacactgcc 21661 tgtgttcaaa tcctagctat gtcatttgtt agctgagtagcctggaacag gtaaattaat 21721 ccctctaatt ctcaatttcc acatctgtta aaattagcataattataatc atatcaggat 21781 aattctgaca ggattatagc aaaaaaagat taaataatatctaatgactg gcacaatacc 21841 tgcctcataa atataagtct tcagtaaata taaaccaccattattaaata ggagatgtta 21901 agaattgagc tgagctgggg agggctgagc tgccagatgtgggagtattc agtaagacaa 21961 caagccaaaa tgaaagtaac gattaagctt ttattccacttactgtgata gtataatcaa 22021 gagtccagaa agaaagtgcc agcttcacct gtgtttcattttccctacaa atggcaccac 22081 gcaagggtca gatgcattag tacagttttg gataactgtcttactgctca gggaacccca 22141 aacaaaaagt tcctatggtt ttatggacct aagggcaggagaagaagcaa ggggaagatc 22201 taggaatggg aaagtaccaa gtactaagtc agaatggagaaaagtgtcct ccagggtacc 22261 cactcctccc aacaagcagg tcttggcaga ggctcctggaaaggttcatc tcagagtccc 22321 cagtaaagaa gcctctgaat ggaggctcct gggctaagaatgcaaatatg tataaaacat 22381 ggctggccag gggtcctaag tccttgactg caactcccgtcagacactgc agcacattgt 22441 ctgtgcaagt cagtttgggg agggaagctt tcctctgtgaaggcctccca ggcaaagcct 22501 ttgtattgcc tagggcaggg caaaatcata cagaggtttttgacttggag ctggattcct 22561 tgagaggatt tctaggagaa atcctcccaa aagtttcaacagtttatatt tcatttgtat 22621 gttggtggtt ctctttcaag tgagatataa tatagatgaaaagtatctaa catagattag 22681 gtattactct tattattact atagctatgg ttgcttgtatataactgttc agattatagc 22741 tccaataaaa caacttattt tattctctcc agagttaggtaatatcatta ttcctcatac 22801 tgaaggcttt tgaaaaatgt tccttggtct ttactatacagagtttaacc tttctgctct 22861 caggaagaaa gacagttgga aattcaccat ctaaatttaaaagggggaaa aatctcagct 22921 gatagctggt aacacagtgt tggctatgta ttgagatacttacttaaagc atcatataag 22981 aaatgatgta caggccaggc gcggtggctg atgcttgtaatcccagcact ttgggaggcc 23041 aaggtgggtg gatcacctga ggtcaggagt ttgagaccagcctggccaac acagtggaac 23101 cccatctcta ctaaaaatac aaaaattagc caggtatggtggtgtgtgcc tgtaatccca 23161 actactcggc aggctgaggc aagagaatcg cttgaacctgggaggccgag gccgaagtga 23221 gccgagataa tgccactgca ctccagtctg ggcaacagagcaagactctg tctcaaaaaa 23281 aaaaaaaaaa agaaagaaat gatgtacaat cactttaatgtgtattaagg ttcttgttta 23341 aaaatcatct aaatttgttt agtatccaat aaaaattgaagtctcacttt gtgccaataa 23401 ttatgtattt aataaatggg aagaggaaac aaaacagtaatttttcttga acacgtggaa 23461 atttatagat aattgctgca ataaatatta tcatacaaaatatatattta aaaaacttaa 23521 ctacaatatt actgtaaaag agaggaacat gattctatattacagtgata tgaggtagcc 23581 tggggtgggg gcaggagatc aaggagagtt taccccaaactacaggtgtt tatattgaga 23641 tctaaaggat gaggaaaaat tactttacta attcattaaagaattgcagg tgtcttaaga 23701 ttgttccagt caaggaaaac tgtatattca aaaccactgattctcacatt tggctctact 23761 ttggaatcat cttcataact ttacacaata ctgatgcctagatttcatgc ccagattctg 23821 atttcattgg catggggtgt ggctttggtg tcgggatctttttttataac tcccctagcg 23881 gttctgataa gctttagtca gtgctttaga aacaatgtttaaattttcaa ggtaggaggg 23941 ttatgcctta ctcaagaaac cgtaggaaga ctcatatgatttgaattcaa agaatgagag 24001 ggaaggcatt atgacatgaa tYgatgaata taaataataacaaatcatgc aaattctggt 24061 aggccacatc caaagtttgg cctttatctt gagaatactggaaaactatg gaaagacttt 24121 aagcagatgt tataatctca gaatttatgt ttgcaaagaaagactgtaaa gagaagctat 24181 gaaattagct tcggctagga tggtaggaag gaacgagttaaatagattca ggagacattt 24241 ttaaagaggt gaaattggca gagctgggca aaggattggatctgagaggt ggtgagactg 24301 acagcttgat ttttggctag tgggactgag ctgatagtagggatagatag tgagcttaat 24361 cttagagata tctgagctcc ctttgaggta tctaagcagagacagtgaag tgatagtgaa 24421 atataaggag attgctgagc ttgagatttt tattgtttaggagtaaagat ctaaattgca 24481 atagagatgc tttacactaa ccttgtcagt ttcataaaaattactttact ggtacaaaac 24541 atggtaaaag gcactgtaat agacagaaaa atgattcagatggtacccat gaccttaagt 24601 ggttaattat ttaatggagg agataggaaa gacagtgagaatttaatgtg attttaaaat 24661 gaacatctta aaatgtctca aggtagaatg gaatagaagagaagaaaatc cagcaacttt 24721 tatcaggttg taataattaa atgctaaaat attattagaaaaaataataa tattgtgatc 24781 tgatagaaaa ggaaagctaa tagaatttga gggaggggaatgaccccgaa gaatcatgaa 24841 aactctcagg gatacaacat catattagga cacggtgtctagttggaaac attttggttt 24901 ttcattcagc ttgaactgga gaaccaaaaa gaaagaagaataaagaggac agcaggagtt 24961 ttaaatcatg gatttgttta ttcaaatctg ccaYgtaacaatacaacaat actatgataa 25021 gagctaggag tacaactgtg gacaaggtag tcatcattctggcacatagt ttataggttc 25081 atatcatatt tattataaaa aatagtttac ttgagacaatatccctagtt agctttcaca 25141 aaggaaacca gtcaaaataa tgactttatt aaaagaggttaagtagccat tctatgagtt 25201 tgcattccct gtgtgttgaa gcaagttttt ttttttcttttttttttctt tttttgagac 25261 agtcttgctc tgttgcctag gctggagtgc agtggcaccctcggctcacg gcaacctcca 25321 tctcccaggt tcaagcaatt ctcccgcctc agcccccaaagtagctggga ttacaggcat 25381 gtgccaccac acctggctga tttttgtatt tttagtagagacggggtttc accatgttgg 25441 ccaggctggt cttgaactcc tgaccttagg tgatatacccgccttggcct cccaaagtgt 25501 tgggactaca ggcgtgagcc accgcaccca gcctgaagcaagttttaggg cagtggtttt 25561 caaccttggc tatacattgg aattacctgg aaaggcttttaaactactga tgccttggtt 25621 atactcctgg ggattctgac ttaattgtcc tggggtttggatggagcatc agaatttttt 25681 aaagctttcc attctaatag tcaacaaaat attctaatagtcaacaaaag ttgaaagcca 25741 cagttttaag ggcatgtttt atcactcaga ctactaagtcacccgaaaaa tgagactgag 25801 caaggaacaa aagaattctg attatatagg aaaaataataacaaacattt ctttagtctc 25861 agtgtgtcat gaacacttct aaacacttaa catattttaaatcagttagt ggtcccaaaa 25921 actgtatgag gaagtcactt ttactattcg catttgagagatgagaaaac tgaaacccaa 25981 cagcacacgc aaagtaagtg ttagaacagg tacttgaactcaatcagtct tcttctagag 26041 cttcttatct gaactggtat gctatattgc ctctaaatgtagtgtaatgg taattttata 26101 ccagaagaat tataaaagaa aggaaaacct ataaaaataggatatcatta aatcagttaa 26161 atgctagttg ataatagagt gattattatg actgcataaaacatctatgg gttatacata 26221 tgtgtatata gacatatata tgtatatatt tattcaaatatgtctatata tagatatatg 26281 tatacatatt tgtatatata tgtgtgtgta tatatatgctggttctcatg ttttcttcat 26341 ttgtaggaat cagttaacaa gttcttgtta catagtatgaaggttctgtt ttcagtagtg 26401 aaacagggag ataaaagtgg ttgaaccatg tgattttttttctgactttt caaagacatt 26461 ttctcctgaa ggaagctcaa gtgctaaggc tattccacataatctcaaga acgactacta 26521 ctttatagta atgaccaaac tgtagtaata gaatcttaggagcatataat catcctctag 26581 ggaacacatt ttacaaattg aacctatctt gcaggtggaaataccataaa gaaaatKatc 26641 attggagaga ttactggcac actgcattca ttcacatgtcaaagtggaaa acattccaat 26701 ttacttctat aaaacatgta tctgagtcaa cctgccatatgtatttttta cactttctat 26761 ccccttacag aatatctacc aaataaatgc tgcacatttctaagtggctg gcttttaagc 26821 tgctttgcct atggcttttt gttccctctt tttctcagctgagaaatcaa gtaaacaaaa 26881 cattgtggct ggtagcatag gtcctcagtg cagtggatattttgtgtccg gcagctggag 26941 cattggtcgt acagatgggc ttcatttgtt ccatatgaatacaagttaat aaatggagag 27001 aattccattt ggtattcaat taagatctcc cctgactcctgttattaaag cctacaaact 27061 attgagcata aaattaaaat cgagcagttc agctctttttgacattataa cactgcctga 27121 aaaataatgt aaaacccaag agtcacaagt cattccttacagcccatggc aaataagaag 27181 tggaaacatt gaggaagaaa gagtaaatat ttaaaacttgatcattagta cacatgttag 27241 gagcaggcta ctgtgctcat gcaaatacat caatccagtttgttgaacac aagatattac 27301 attaatttta acagaatgtt acaagattat tattgccaaagctccaatga aggaaataat 27361 taaagcacta ggcattttaa ggtcttaaaa tgacagaaaatactatagga aatcaataac 27421 cagaaaatac tatagaaaat caatatactg taattgaaaggaaattgaat tgggtttact 27481 ttttttatcc cgtctaaatt ttcttcagat tgccagaccactgaagatag tgaatcttgt 27541 tttgccatca gcagtgtgac tttctctaag tctcatgtgctgcaagggcc atctttattg 27601 ttttttattt aggaagcact cagctgaata gacaStatgagattttgtgt gtgtgttctc 27661 tttactagtc ttggactcat tgttaaaaaa aacagaaacaaaatcaaaga cacagcagac 27721 aaaaacaacc acctccacat aaccagagaa gtcaaattaatgagaattca gacatcgaaa 27781 ccagaggact tgttagataa gcctcacact tgaacctaagtgaaattctt ctagttttta 27841 tcaaacacta tgcctgtcat tcttgctatg caataagaaacattaccagt tgcagataca 27901 aattcaattt gaatctatat gaatctaact cattttcattgcttattatt ttaaaaagtt 27961 attataatca aacaaaaaag cttgaaatga acctcagggaggtaagtgga tatgaacaga 28021 aattaaagaa tgttaaaatg agaaatccag actgggtgctgtggcttaaa cctataatcc 28081 cagcattttg ggaggctgag gtgggagaat cccttgagcccaggagtata aaacaaaaca 28141 cttaacaatt ctgagtctca atgtcgttga ctataaagtagaaatatctt cccttcctac 28201 cttaaataga tcacatttat gaaaaagatt cataagttataaaacaacac ctcaatgaaa 28261 aatgctgtat ctggacttta aaaagaagtg aattaaatactaattttacc tcttagtata 28321 tatgtgatct taatgaagtc cttagttttc ttgtctgttaaataataatg gtaagggcac 28381 cttctcatat ggtcattgtg aagcttacat gtataaaaagctcagtacca tggctgacac 28441 atagaaagta tttaacagtg ttcaaaaaca taatcaatataggaaataag tattatttta 28501 tttccttaat aatttcttag ttatacccca ttatcatatgtgataagaga atgtacttta 28561 tttctagttt tccagataat ctgtatccta aacattctttactcccaaat tcagtctttg 28621 agcatcctaa ctcttgacat ccacagctaa tatttaggaaacattaaaat ttgtttgtac 28681 atttgaagga agaactaagt aaatggaact taccaatcctaaaatccaga ctgctgttat 28741 taagaagaag aacaactgta caaatgctgt taYcaaaggatgtatgagtc aggcggaaaa 28801 aaaaggagaa cacagaaagg tgaaaagtct ttgtcaagcagaggagagaa attattgtct 28861 tgtaatatat atgagagagt ctagccaaat attaaacatttttaaaatga aaagaaatat 28921 tatacatggt atgaataata tttaagtcat tgcctaggttagaagattag atatatttac 28981 ctattctacc tacttcaaat gcttttttgc tgtcattgtttggaacctcc aaatactaga 29041 atgctttaga attataaaac cattgaccat gttcagttatgtttaaaaga aaacatcatt 29101 ctgaggaaac tggaaattat atatttataa atgtttgtccatagcattgt tgaaacaaat 29161 ggctcaggtt tcactgttaa tccagttgct gatatttttgactactcaat acaccaaaat 29221 ggtacagtca agttggtgct aatagatgtc atttatttacacaaactgac aataggttca 29281 ccaaagttaa gagacgaatt taagggggac agaaaagaaattcctgtgtg tgctttggca 29341 gcttagacat acttaccacg aacaagatta tatcttaaaagctattcgtt aagcttaaaa 29401 ttaagttata tggttgtaat atgtggagaW agcacattggaatagctctt cacttaatgt 29461 tatagatcac tttttccacc aatattattt ggtctaggaaagtaaaatcc tcttgaggaa 29521 gtaattagaa aaagcacaca tcagggaatg taattgaagatagacttttg ttgacctcag 29581 aagagaaaag ctcaaaagaa atttcgtaaa gttttaaatatggcaagagg accacaatgt 29641 ctttgataat tagactttca acacatttgg atttgctaattggatctaag ttctaattag 29701 gctttttatg taactaagaa tgacatatca ttgtatgaatttaatgtgct tttacagttg 29761 aaactaacaa ttagcagcct tttccaaatt attttaataaggaaaaagct ggctctgctg 29821 tttatactgt gacattccct tctcttaaga tatattatgtacattttcat tttctWaatt 29881 aaacaccttt tggggagctt aaagggaaac ttgggttttaataggaagca cctaaattgt 29941 ttatttgtgg gggagacttg ccagccagta tgtagatgactttggaagct acagacaaac 30001 cgcaataaaa agaaataaat cacaatcatc aggaggaaacagcacaaata accatttgcc 30061 cctatgacca ttcagttaat caaaacagtc tctgaatatgatgaaagatc tgttacattt 30121 ttaagtgtgc acatgcggaa tatgttccca aatgtgaaggctcatggatc tgctttcagg 30181 atgaataata ggaaatttct tctcaagatg tgcattacaacattctctct ctctgtagcc 30241 ttgttctgta aacagttatc tgctaattca agtcaaatcatctctttctt tctccatatt 30301 tttgattgag agtcatcaat ttttatccca ttcagatatatagacagtaa tttaaaaact 30361 ggtRgccctc agatttgtac attccaagag ataagacctatataacagca taaaattaaa 30421 ctactatttg ggtgagtaaa gagcagtgag tcattgcaagactttaaggg aaaggctgct 30481 gccaaaatat tccaagcaaa ttgataacac tgccaaaaaggcaaattata ttgttttatg 30541 tgctatgaat actttgaaaa caaagacata cacatgatttttttcaaata aaagcagttg 30601 cccttctaac catattaaaa atagtgtctt ttttcccagtaaaagccata taagatcaaa 30661 ttaaaaccat tgaaactatt agagaatcta acttacagtaattgatatat taaaaaagac 30721 attatgttag agttatatgt gctattattt ttccaattagtacattactg ggactgagtg 30781 attggtgtat acataagtcc tcattaagta aaatgtcctcaatgagcctc ctaaagcaca 30841 ttgggtactg tcatgtgcca tataatgata tttaagagcaagaaatggca tatctaataa 30901 cttcatttcc tatttaaaaa gaaaacctgt gcagtactctttccatacca attattctaa 30961 gagctatcac atagttctcc ataaagacta tacagaggtgatgggactgt tcttcaggca 31021 gaaaaatgac caaagttgtc ttatctttca gcttggKtgctggaatcctg tcacaggtct 31081 gaatgggtca ctgactgaca agaaattgga gaataacatgcgtggagtgg ttctacgtgt 31141 agtaactgtt ctggtaagta ttatctgagc ctcgtggttttgacttttgg aggttacttt 31201 atcctatatt tctttcttta ttaactgatg acataatcttaaaagagatt ttaacaaatt 31261 ctaaatacgc tttctaagat tttttaatgg tcatatttcacagccttcaa tttacgtgga 31321 tcaggatcac agttttgcag acttttaact acagtgtatgtgttaggaat tttcttagaa 31381 attttaggct caatttccat cctacagttt tctacacatgacaaagtaaa ggtatgtctc 31441 aggccttctt tcattaaaac tgtagtgaac atagatagtcaacatatagt gaacattgaa 31501 actataaatg aacataaccc tgaccatgcc aaacaatggtagatttggta aaatgtacat 31561 tctctaatag tgaaatatta acaaactaag aatctttttctcgtaagaac ctacttcaaa 31621 gggaagctgt tgtttaatag gcatctactg tcgactgtattccaggctca gtgctgatta 31681 ctgagaacat tgacaaataa aatgacagat ataattctgtcttgatagaa cttatcatct 31741 agtgattctg tgaatgttga ataggattac agtagtttcccYttatctgt gggggatgca 31801 ttccaagacc cccagtggat gcctgaaact gtggatagttttgttttctt ccaacacaat 31861 cacattgtgt agggcttata ggttatagag tgtggatatgctggacaaag ggatgagtca 31921 catccagggc atgtcagtgg gatggtgtga gatttcatcatgctacttag aacaatgtta 31981 aattcaaaac ttataaattg tttatttctg gaagttttcatttaatattt tttggaccat 32041 ggctgattga ggagtaaccg aaactggtga aagagcaaccatggataaga agagtctact 32101 gtatacctgg gaaagatctg ttacaatttc atttacttattaaaaagtca ataaatggta 32161 actgttataa ttctatacat tatagttttc tttcctaatagatacataca ataagatgtt 32221 aatagaaata ttcttaaaca gtaaaatact caaatgggctctgtctaaat agccataaac 32281 cacgaaggca tacattctcc tttgctattc caatgtgtttaatcatgtgc tagtttatag 32341 cctgtttcaa aacgtaatag tccatagtta gtactttatttttcttcaga aaagtctact 32401 tgtttttaat tttgaaaaag attttcctat gggctaggtaaattgcagaa ttagtaaata 32461 ggagaggaag actttttgat acgttgatgc taattggatgttcatcaaat tttataacac 32521 cagttgctgc cctttgtaca ttgtttacta caaattaaatatatgcaatg taattttatt 32581 gtcaaaaaag tggatttgtg catattttct atgaaaggacattcaagtga gtgacctatt 32641 cttctttttt tgtggcccat aaattattcc tagttattatttttaaaagg gcttaattgc 32701 ttttggtaaa taattgtaag tatttcacaa cttaaatgtgaattcgcttc tgtatgtacc 32761 taagggtatt gatatgcatt tacattgatg cactaatattaaaaaagaca gatatttaaa 32821 gtacagcaaa ttattaatag tctttcctcc agtgattagcatgaaaactc cagtgatgat 32881 tgcagtgaaa gacggtatat gtgctttgtt ttcctttgagaaaagaaagc aaatctttaa 32941 gtccaattaa cctcagtcag ttttaagcaa atagttatttcgcaattact atatgataga 33001 ctcagtaatg gatataagag aaataatata cagaccttgtcctcaaagta ttcatagaat 33061 cttgttaggt tgatgcacat agagaaaaac agctcagattgattaaatat ggttgaccct 33121 tggtgtctgt gaggcattgg ttccaggaaa cccccacaccaaaatctgca aattcbtaag 33181 tttctgatat aaaatgatat agtatttgca tataacctatgtacctcctc tcatatgctt 33241 taaagcattt tagattactc ttaataccta atacagtgtaaatgctatgt aagtagtggc 33301 tctaccgaat tatatattta tttttgttat ttttattgggtttttttcct aatatttttg 33361 agcctcagtt tgttgaatct atggagttga gtctagatgMagaacctgtg gatatggagt 33421 gttaactgta cttactatgt gcaagctact ctactaagtcttttgcatgt attgtcttat 33481 tttgtcctca caatattctc aagaaatagg taatattctgatctcaagag gaaactgaga 33541 catccagaag ttgaaataca tacatgtatg tgtgtgtgtatatatatata tatatatctt 33601 atcattaagg gaaggggtta aggtctagaa tcagacttcctgaatttgaa tcctaactct 33661 gctacttact atctgtataa tgttcataaa tacatgaacctagaagaaat tgaagaataa 33721 catgcaagga gcgattctac gtgtggtaac tgctctggtaagtattacct gagccttgtg 33781 gttttacaga cagtaagtag cagagttagg attcaaattcaggaagacag attctagacg 33841 ttaacccctt ccctcttaat gatacattat actgtctccagtcctactgt gataagtgtg 33901 agaactgagg tgtctataga attttgtttg acttgagagttgaaaatgct tacgtctgaa 33961 taaggtgaaa gtgagtttta ttgggaagga ttgtaggtacaatcatgtta aattcatttc 34021 caggctgaat aaaacaattg ctattttgtt catgaaatagtagcacttca cacagtgtta 34081 acacctgcat accccaggtt caccttcatg actcttaataatctctattt cctgcaagct 34141 tagccctgca cagatattga atatggtttt gtgactggagacatggaagc agtgtttttt 34201 tttttttttt tcaagtcttc tatgatggac ttatcagttgggtgccagtt gagtaacagg 34261 ttgttatact tattacatta ctacaatata caatcattgtgtttaataat tttgcatgct 34321 tcctatgtct ttctgtaact actctgagaa tggaaaccagtcattccctt cttatttatt 34381 ttctacaagt gccttttcag gtctagctct tagttatatggccttgcaag cttcttctac 34441 tcaacactga gaaccactct cccctcaccc cacccagcaactcccgaatc ttgaggcttt 34501 acctcactaa accctgaaac taggatttaa tctatactcaacttgatgtt gtcacctaaa 34561 cgcaacctcc caccccctta tattccaagt atttacgtccaacactttcc agaagagaaa 34621 agggaaagga ccaatgtgta catgggaaga agctatttcctcctttcttc tattgaaact 34681 gaaatctagt cttagctcta cccactccct cttttcttaagaaattttat attatcaatt 34741 acaccaggtt aataaaatgg atttttctta ccaaaattttgaaattccca ttcttatgac 34801 tgttgatatt ttattgactt gaaagaaata acttcatatttattttaaac tacacaattc 34861 agcactctac tgtttgtgat cccatgggca aataagtggtgctctcaaac atttcattga 34921 agagttaaat agaggtatta ttgacgaaga tgtaaatagtgttaaaggaa tcaacaagga 34981 gtgttgaatc accagaacct acctagatga cttacatgaagactgataag gcatgggagg 35041 aagcattatt actagacaaa gcaatagagc catgggagagagaccatcac tcatagtagt 35101 agtgagctac agtgcagaaa aagaaagaga atcactgccaataccaaaat caggctatga 35161 ggaactggga ataagggata cactgatctc actcctcttatctatctctt ttcatggtct 35221 ccaactgcaa atccaaccag aagtcagagg ttatgggagcctaagtaagg aagaccatga 35281 aagttagtct ttcaggacac agaggaaggc agtgaagccctcagtgaatc cagaagaaaa 35341 gtagagaaaa accaacacga gcatctacca tttgataggacctattataa gaaggttata 35401 tatattaact tatttaatcc ttataaaatt tcatatgagtggcactaatt acattataag 35461 gataaaaata gggtacataa gagttaacta aatttctgcctccatatagc tagcaggagc 35521 cagacataaa tttcaagatc agagatcctg gttccacaaaccatgtcctt agccactctg 35581 ataatctKcc ttctgaatca gaatggactc atgttaggtataaatgaaat ttgatataat 35641 tattgagatg tgaggattgc atatgttgga ttgataacttaggagaattt tcctaaattt 35701 cttcatatat agatttcttt gtattcaacc atcaccaaagccacagaaat atgtcttaga 35761 aatggaggaa aagaaagaat gataataaaa gcagtctctactacttggtg tctcttctaa 35821 tctctttgtt tccataattt gcccactgat ctttacttcttaccaagtgg tccaagtgta 35881 ttactacaaa ggaattatga agattttccc gtttttaacttcctgagtta tgcaatgtag 35941 atagcaataa atgaccaatt aagtcattca attgttttcatggtaattca tgaaaacact 36001 attggaaaca ttactgagtt ataaacataa tcataatatatatgataata aaatggaaag 36061 gaattggtta caaattaaaa gaaaggcgtg cgaagctatgctgcaacaag tataagatat 36121 ctgagaacca taaattggca tttgggagga gctcactgattagtaattct tatttaccta 36181 ctttgattgg ccttaaaaaa gacatataca ttcctaataaacatacattc actcatgaat 36241 tcacacaaaa cagtcaaaga tcctgacttt agagagtttttttgttgttt gtttttattg 36301 agacaggctc tcactccttc acccaggctg gagtacagtggcatgatcat ggctcactgg 36361 agcttcaaac tcctgggctc aagaaatcct cccacctcagcctcctgagt agctgggact 36421 acaggtgcat gccaccgtgc ctgactaatt tatttttcgtagagacaggg gctcgctatg 36481 ttgcccaagc tggtcttgaa ctcctggcct caagtaatcctcccaaagta ttgggattac 36541 aggtatgagc caccgcaccc atccagggag tttgattcttgcaagatatc ttctaaagct 36601 ctcaaaatag tcttgaaaaa ctgcagtgca gttgttctccattaaataca caatagagca 36661 tttttttgcc tagaattacc ataaaaataa ttcaatatataaattgaatt catatataaa 36721 tatatgactg gaagtctata gtaaagactg tgatagtgatttttctgcat tttataaatt 36781 aaaaaacata gtttttcata ttttaatctc tctaaaattaggatgcatcc ttcaacttct 36841 atttgcaatt gtaattagca ccatgtttgt ctttattagtgatgtgtaaa atagttgcac 36901 atcttacaat aggaaacatt ttagattaaa taatgtatggtatttttaca aattaagtta 36961 atggatattc ctggaaagtt aagttaaacg tcactgggcaagaagccaca taaagagaat 37021 aactatttta aagaatacct ccaaaagaaa aggcagtaggaaatatacca attagaaaac 37081 ataggacagt aatagcaatg agaaaaatta ctgtctaaattaaaactgtt gcctcaatca 37141 tagaaggata tacacagaaa taggaaatac acagagatagcttatcttga atcattacca 37201 caaagcaata tccaaaatat ctattgagag aatggtaggtttttctaagc aactcaccaa 37261 aggaaggatt gtttgcctgg ctgcagcaga cagccacctactgagttatc attttgagta 37321 atttcttttt cctaaaaaat atccatcatt agtctcactttttggaattt cttttttttt 37381 ttttatggag attgtgactg atatatgggc accagagtcttaatttgtac aaaaacaaaa 37441 aaaaagtatt taggttagat ttagttattt atatacatcagcatgaagta aagcaatgta 37501 cttaatttcc tcttaatata ccttttaaca aaggaataaataatatataa ttcatttttt 37561 attcaaagta cttcacacaa ttttgttgcc tttatttcaccaagtctcct ctatttaata 37621 cacctatgct aagaagcttt ggcacacact gcaaaaagcactSaacttgc agtcaaaagg 37661 tatgactttt agtatcgtac tagtttttct gcttaattgatctttgaatt tatggaaacc 37741 aagacgctgt tctgaacttc aatttttcca tctgtaaaacaggaataata aaaccaatca 37801 caaagggtta ctactatatt aactggatga aactatgtgaaagcaggtag caaaatgaac 37861 agtgYttcta catacattat ctctgcagta aaactatgatgtgtaaactc agtaaagaaa 37921 aaaaagcatg agtgatcata aaagtctaat tattcattgtcatcaaactt cagatttttg 37981 tgggagattg gaggactcta agggagacaa acaaatgtaaactgcagaat aatgagtttt 38041 aggccccgtc actgttacct caatttgaaa acctgctaatgaggaccaca ggaactcttc 38101 tgccagtgaa gctggtactt cctagaggaa aaatgcacaaaaaacaccta ccaccgaaaa 38161 caaaagcacc ctgaattatg attttcaaaa atatctagaagtttatatat caattatWtt 38221 atctcatata aactgtgttt gattccaggc tagctttaaatagactctat attttaatat 38281 ataactaaac aaatgcctcc agtgatagtt ctgaaacgtcattgtcctta aattttttgc 38341 taaggacatt gtccaagtgt ttgctactta gagactttcagaattttcat cgctacctca 38401 aagttcaagc ttttctgagg tcccagcgtt tgagcctcttgaacctggag gcaaggttag 38461 gggttgggaa agtaaacttg tcaagagact ctactgggcaagccctcaca gcaaggagtt 38521 gggaagagag ctgacagcaa gctctggtgg ctcccacagcaatgtcttac gagggctcct 38581 tcggaggagt taataaataa taaagtttgg ttcaggactccaggcaagaa tgacaactct 38641 ggctgaggaa atttccaggg gctgatatct aaaataaacttaaagtcaca ataaaatatg 38701 ataagtgcca tgtaataatc ctacaatgga aatacacaggatagcattgt ggttaagaac 38761 acagttccca cagtttcact tctggggtgg caaatgaggagttccatgga cccactcccc 38821 agcaaaataa gcagagatgt caaaaatgaa aaagaaatgaacaaaaaaca acactaacaa 38881 aaaagtttac attctctgga aataattgca aatcatatatctcataagtg atatttagca 38941 tatacaaaaa tattataact cagtatgaag aaatataaacaaaattaaaa aaaattaaaa 39001 tgggcaaaga tctgaacagt tctgcaatga agatatatttatataaatgg tcagtaagca 39061 aatgaagaga tgctcagcat agttttccat caaggaaatgcaaatcaaaa ccacaatgag 39121 ctatgactac aagcccacta ggatggctat attcaaaatgacagataata acaaatgttg 39181 gcaaggatgt tgaaaaatca gaatgctgat acattgctggtaggaatgta aaatggtacg 39241 actccttcag aaaacagtct agcagttcct caaatggttaaaagtagagt cactacatga 39301 ttacaaattc cactgtatat ataatcccaa gagaaatgaaaacatatgtc catatgaaaa 39361 cttgtactca aatgYtcata gcagcatggt tgattgcagccaaaaatgga aacaacccaa 39421 aggtctatca actggtgaat ggatacataa aatgtagtataccataaaat ggaatattat 39481 ttggcaatag aatgaaatta catgttgata acagtgtgcagcatggatga accttgaaag 39541 catgctaagg gaaagaagYc agacacagag gatcatgtattatatgaatt catttctgtg 39601 aaatgttcag aatagacaaa tctataggga ctcacaggggcaaaaagtaa ttcgtgattg 39661 cctaggtctg ggaaagtttt ggagattggc agatgacagctaacaagagt ggcatttttg 39721 agggggagct ggtaataaag ttctaaaact gactgcaatgatgattgcac atatttgcga 39781 atatactaaa gctattggat tgtgcacttt ggatgggtgaattgtatatt atRtaaatta 39841 tatctgaata aagctacttt ttaaaaaaga gagagtctggttccccagac tgcctatgtt 39901 taaatcccca cctctcctac ctgtgaatgt aggccaggtatttaaactct atgattctgt 39961 ttcttcatct gtacaatgga gataatataa attacctacctcataaagct gttataaaaa 40021 ttcaaataac tagtacatgc aaagaacaaa gaccttctaacagaacctgg cacatgaaaa 40081 gtgtgatatg tgtccattct tcttatgcac agactgttaagggagagcat gaaaRaagta 40141 tccaacttat gggtaaggca gtcggcaagg cttaaaggatgatatttaaa ttcatcttga 40201 aagatgagca gggattatca aggaatagat aagagggaagggatatccag cattagaaag 40261 agtatatgcg gaagtatgta gatatgatat tctatcaagaatttaaagta tcctaaaagt 40321 aaattcaaat attttctgaa gtatatgaaa ttgaaatacagaaaattcca gcaacctatg 40381 tgtcatgtct tctctttttt aagagttcct ttagagacaattcagtaaga aatttctctg 40441 tgactatgaa ggtaatattg tatgatgtaa ataatattcaccaagttaaa aataacattg 40501 ctacaactct gatccagtgt aaagtttagg taaaatagtactcattttta ttattgaaac 40561 agtgtaatca gtagacatag ctctgtgttg tgtacatattttgttttgtc tcttcttctg 40621 agtgtactca tcttaggtga atggggaaaa tatgatttttataaacagac tcttaaagaa 40681 aaatggccta tgcaaatgat gtaatttaga gcaaccatagtatatgtgtt cacttcagcg 40741 ctcactaaat aaaatcacca taagaattga tgcatgacaatggcttgaca atttcttatt 40801 cttttgtatc acaccaattc ttgaaatgat agctgttgcacagacatgtg tgagtattgc 40861 tattgtatat gtggtagcca tgcatgaccc tacttttctcctcttggaag ctggaacctg 40921 aattcttaga ctaatcaatg agtaaatatt tcagagatgtgttatctaat ttacttctga 40981 aaaacagatc tactacttgt ctctagacat ttagaaagagattgacagag tcatttgcaa 41041 attcaaagat ataaaaacag taggtagatt gacttccataatcaaatcaa tatttggtta 41101 gaacatttca caatattcta accgaaatta catgaactacattatatact ttcagaaatg 41161 cagtgtacca ctccatctga acaaaaacat aatgttaaggtgtccttgaa gctttaagtc 41221 atcttttggc aatgcagtag tttcaacata atcacaagacaaaaccagtt ccacataaaa 41281 ttcttcagaa atgaacatga atataaataa aaacttgagagatgctactg aaatagattt 41341 tacatttcag agttacaaag gaacacatta gtagctattaaagcttacag ataacattac 41401 catctagctt actaataaat ctcattgtta gtattttctctagtctttac tgaaccttta 41461 agattattaa tttttttcat attttattga tatggctaggatagagttat taaataactt 41521 cttcaagtta caaatatgct aaaaataagc attgaatccttatctatcta atttactccc 41581 agccatgatg attctaacaa ggactggcct aatttcctgccacaaaaggc tataaaactg 41641 agccaagtat ggaaaaacct tttcagacat tagtcaattggtagaaatgg actttgaYac 41701 ctaaaaggtt ggaaacaact aaatgatccc aacaattatcccagctttct tgctgctggc 41761 agtttccata ctggagacat gaaagtatgg atccaaaacagaacttgtca gcctcactga 41821 gtttacgaag tagagatcag agggtgagaa agctgatgtggttggcgcat attatctcat 41881 ttttgagata tgttgtaaat tagagggagc tgagcaggaaaatatttaca gaaatgtgaa 41941 tcttgcctga gtactagacc atcaatgcta gagagaaattctacaagtgt gggcaaagaa 42001 tgacaggaat cagaacaatc gccagagctc acacatgatttttgagaaag gtttcatttg 42061 gggagatcgt attgtatcaa gaattcaaaa gaaaatgtagaaagtatgta tttatctttg 42121 agttgtgtag tgatcgatag aaatttaggt gaatatctaaatatctatat agatataaag 42181 taggatactc ccacacacct tgctatttct gtgttttacatatttccctg acctcccaaa 42241 atgtccagYt ttttgttagg aagctgcacg ctcatcttccccttctgagc tccaagagca 42301 tgttgatgtt cttgctgttt catagctgat ataaattagcattgggtaaa cgacgaaaaa 42361 acgtcacaga aagtttttct tgttcacatt ttggaaatcttccttctcag tagagaaaag 42421 caaccttaaa gttgcaacat catgattgga tttcagttcacaaaagaaaa taaattttga 42481 caaatcacat atgcttttcc tgtccctccc aaaatgctgcagtcttccat ggcagcctta 42541 ccaaagaaaa aactaagtcc ctaactggaa Sttggattctttcaaaggca cagtaagagg 42601 ttttgaccaa tagagaagca ttgatcaaat cactatccactgcattacat ggtactctag 42661 gagagtctga aatgagaata gatatcttca aaaagcatccaaatctgggg ctggtttact 42721 catctgtcag cttctggaat agtgaacatg ctattaaaattcagctcctt ctgggaggtg 42781 ggaagataat caaggggaga cccaccatac tgcatggaagtcccagaaat ggaaaagctg 42841 atcaaaggaa ggaaggtaat atactttttt ataatatgttccttgggatt tttaatgatc 42901 ggtcgaaata cagaggtttt ggttttaagg tgttattttcacagcatctc caggtaaact 42961 tccaagtatt atgttaRcct ggatagaaac aaaaaagtccataatgcttg aaactgaata 43021 tgtgatcctt gcatcatgtt taaaattgtt cagcaaggaatttcagtttc tctaggtgat 43081 ggaagttagc acgagcaaag gaactttaca atttttcagaattttttttt tttcatgttt 43141 ggaaggtctc ctcctcctcc tcctccttct cctcctcctcctcctcctcc tcctcctcct 43201 cgtcctcctc ctcctcctcc tcatcctcct cgtcctcctcctcgtcctcc tcctcttcct 43261 cctcctcctt ctcctcctcc tcctcctcct ccacctcctgcttctccttc tcctccttct 43321 tcttcatttt tatttttaac cattgctagt ggtatgatttttgaattggg aaaacaaaat 43381 attcagacta aatattttcc caatgattct tatgtagagtaatgtacttt gcaattcaat 43441 taaaaataac aagctaggat ggtgttctga gccaaatgtaatctccaaga ccatggtcag 43501 taaagtactc aagaaaaaag tattgaggcc gagtagtctgaagtctaYtg gggaataaag 43561 aagcagctga ttagcatttg aaagcaaggt ctttcactgttttaaaagtg aaggagaatt 43621 ttttaacaga Rcactcatca aaaacaaagc gtttgaaaattacccctcct ttgttttatc 43681 agtctcaggg aagatgcact tcgaYacacg tctcaggaattaagatgcat ttcgatacat 43741 gtaatattac atttggtgac actgctggaa catatgctgagaaatgcaag aataaatagg 43801 actaatgctc attttcNttt tttttttaat cacttgcaagtgacatttgt ttcatctagg 43861 caggaaagca ggatcttata ggcagggtag gtgtaagggctgattttagg tcaagctttt 43921 caatttatta tgcaataaaa tatagcaata aaaatatgcatctttatatt ttccagtaaa 43981 tbataggaat ctccgtttaa ttttttatat atgtgctaataattaattca gtactacagt 44041 ctggatccac agtccttttg ataagagctg gcacagaggtcatttttatg tgccttatta 44101 ttaatcttaa taatatgtag taatattcct ttatatgcttattgtgacac acagaaactt 44161 aaagcctttg tagctttaag ttcagatgtc tccacattcttttgtaaatc cctgcagatt 44221 tgtttcttgc ttttaaatat cagcaccttt ctgctgtctactcttaggtc tctgccctct 44281 atgcaggaga tcttttcttt taagtttccc accgtgcttcataataaaat caaaagaaaa 44341 aggtacatcc ctcagaaagc attcatctca ctaKaaatagaaaacctgtc accttattct 44401 taaaacctta gatcagccag gccagtggct gaaatagtgagggtagtgtc tacaaagggt 44461 ataKagtagg ggtagcaaaa taattatgta ccttgttctctccacatctt ttagggaaga 44521 gactctttgc tctggaaaat gacttaatac cattgggaggaaaacatttc ctctacccac 44581 ttaggttcct atgtttaggg gcctgtgaat taactgataatagacagatt aacaggagaa 44641 aagaaaaggt taatctacac atgcatgtga agtcactcaataatgagtcg tttcctgaat 44701 acccagaggt aaagattggt atagcaactt aaaaaagggaagggaagtgt ggctagggca 44761 tcaatgtgta gttgatctcc ttcctggRat tagcagaaagctcttggagg agagttaata 44821 gcagctgtat tgggggtagt gtcagcttta gttaggtaagggaaagtcag atcagatttc 44881 aaatattttc actttaatct ttatgctaaa actgtgattccttcaatatg ctgatgaaag 44941 ttgaagtgtg gcttggaaaa aaagagaaag tataagtagatttattctag atataaagca 45001 ttttttaggt atagtagaaa gagagaaaga gaaagcaacaggcacacaca cactgagaga 45061 gagattttga cacgaccatg tcaaagacta agagtaccccaagttcttta tcaaccatgt 45121 agtttctgca acctgcagta tacacacatg catacccatatactgatgga ggcagtctaa 45181 attaatggtt aagaatatga acttcagcac cagagggcattggattcaaa tttcaatctt 45241 tcttactagc tttgtaactt caaacttttt tcctcttggaagctggaaac tgcattttta 45301 gattaaacaa tgagtaaata tttcagagat gtgttatacagtttacttct gaaaacagat 45361 ttattacttg tctctagaag tttagagaga gatcgacagagtcatttgca aattcaaaga 45421 tataaaaaac aggtagactg actaccataa tcaaatcaatattggtttag aaaatttcac 45481 aatattctaa ctcaagttat gtgaactata ttatatacttttacattatt tatctaccct 45541 caagtttcag ttctctcctt tgcaaatgac aataacaataccaaaaaata aaaaaactaa 45601 atgaggtaat gaacgtaaag tgataaggat gatgacaggcataaagtaag ctttttattg 45661 tttttatttg ctttttcctg tagcctgaaa atatgttcaaggtaaaaaaa agcattcttt 45721 tgctctgaat ctcctttatt ctaccaattc ttttctcgtgtaagtgtctc agactcttca 45781 gttgtcttca ctttgtcatt tatttttatc ctcaaatcattgatatttgg cttctatttt 45841 cacttctcca aacaaattgc ttttttgtct tcatgatcttctacgtgaca aattaaatgc 45901 atgctttcag accttacctt cctggacctc tcacctccattggaatccac tggcaattta 45961 ctcccagaag ccctgtcctc tcttgcatct tcaacatcttctgattctga cctctctatt 46021 cctttgttta tttgtttgtt tgtttgagac agagtttcactcttgttgcc ccaaatggag 46081 agcaatggca tgatctaggc tcactgcaac ctccatctcctgggttcaag tgattctcct 46141 gcctcagtct cccaagtagc tgggattaca ggcatgcgccaacatgcctg gctaattttg 46201 tatttttagt agagacaggg ttttgccatg ttggtcaggctgatctcgaa ctcctgacct 46261 caggtgatcc tcccgccttg gcctcccaaa gtgctgggataacaggcgtg agccacagtg 46321 cccggcctct ctattccttt tacctccttc attggattctcttcttccag agtttcatcc 46381 ctggccttca ctatacttta ttgaatgact tcatttactttctagaaagt tataaacaaa 46441 tttttatttc caaccctagc ctctctctcc tgcttcagactcattatcca cgagtactat 46501 tgaaaaaagt attctatatt taatgacaaa aaaacagaaaatatggaaaa caaaaattaa 46561 cagcaaaatc ataaataatt cttccatcaa aagttacttgctaatatttt agtgtctacc 46621 actagctaaa cttaactaca tttccttatc tataaaatttgaggtaattg aggtcccaaa 46681 ctcataaaac tattgagcat gaatacacaa aaagtttttaaagtacccag cacatactaa 46741 gttccatatg aatatttgct gctattattt ttttcaatttcaaacatgta cacacatgtt 46801 tcaaattcaa aaatgtagct ttgtacattt tcttcccatttagcaatatg caaatgagta 46861 tttttaattg aatagatttt tttttttttg agacggagtcttactctatc actctgtcac 46921 ataggctgga gtgcaatgcc gtggtcctag ctcactgcaacctccgcctc ccgggttcaa 46981 gcgattctcc cgcctcagcc tccagagtag ctgggactacaggtgcatgc cactacacct 47041 ggctaatttt tgtagtttta gtagagacga gtttcactatgttgtccagg ctggtcttga 47101 actcctgacc tcgtgatctg cctgcctcgg cctcccaaagtgctgggatt acaggcatga 47161 gccaccgtga ccagctgtga atagatattt ttaacagatgtgatcagtag attttttcag 47221 ccacttagtg aataatttct acagctgcct caaatgtctattaatttatc tagggttgta 47281 tgtaattccc agccttctaa ctgttgacag ttttatttctcattccctga actatgttga 47341 aatgcaaatt aatgtcttta cagagatatt tttgaatctctctattaaaa aaaaaaaaag 47401 aaaaaagaaa aaaacaaacc aatcccgaac ttcaaaatgctattcttggt tttttaaaaa 47461 atgacctata gtccaactct gtgtagataa ttatgtgacagggcattgag acactgaagt 47521 tgacagccca atgtgctgtg ctgtgtcata cagatagacttaaaaaaaaa aagccccagg 47581 ctgggttctg tggctcatgc ctataactcc agcatttggggatcacttga ggtcaagact 47641 ttgagacaag cctgggcagc atagtaagac ccatcccccccccccaaaaa aaaataccag 47701 gcataacagt gtgtgcctgt attcccagct actcaagagtttgaagcagg attgcttgag 47761 cccaggaggt tgaggatgta atgagccgtg attgtgccattgcacttcaa cctgggcaag 47821 ataacaagac cccatatctt aaagcaaaac aaaacccatctctgattgta gcgtatttag 47881 gttgttttca acttcttcct agataacttg caatgaacgttttaaaaata tatacttgtg 47941 cacatttatg ctcatttatg attatttaga tgtacaattattagatgaga taatataaaa 48001 aatttacagc ctttcacttt tatttttctt tttcttttattttactttaa gttccaggat 48061 acatgtgtag aatgcgcagg tttgttacat aggtatacatgtgccatgtt agtttgctgc 48121 acctaccaac ccgtcatcta gggtttaagc accacatgcattaggtattt gtcttaatgc 48181 tctccctccc cttgcctacc ccactaacag gccccagtgtgtgatgttcc cctccctgtg 48241 tccatgtgtt cccattgttc aactcccact tatgagtgagaacatgaagt gtttggttgt 48301 ctgttcattt gttagtttgc tgagaatgat ggcttccagcttcatccatg tccctgcaaa 48361 ggacatgatc tcattctttg ctatggctgc acagtattccacgatgtgta agtgccacat 48421 tttctttatt cagtatatca ttgatgggca tttgggttggttccaagtct ttgctattgt 48481 aaatagtgct gcagtaaaca tctgtgtgta tgtgtctttatagtagaatg atttataatc 48541 ctatgggtaa atacccagta atgagattgc tgggtcaaatggtatttctg gttctagatc 48601 cttgaggaat cgccacactg ccttccacaa tggttgaactaatttacact cccaccaaca 48661 gtgtaaaagc gttcctattt cttcacagcc ttgccagcatctgctgtttc ccagcttttt 48721 agtaatcacc attctgactg gtgtgagata atatctcattgtggttttga ttcgcattgc 48781 tctaatgatc agtgaatgtt gagcttcttt tcatatgtttattggtcaca tatatgtttt 48841 ctttatagaa gtgtctgttc atatcctttg cccacttttttatggggttg aggctttcac 48901 ttttttattg tgaatttgtt ttctggaaaa cttttaccaattttaattca cataagtagt 48961 gYatgagaaa gtctactttc tcacatgctt gcKgataaatgttaacattc atttttatcc 49021 ttgccagtca gataagcaat aaattaaata gttcttgttattttaatttg tattccccaa 49081 aggtatgtta agagagcaaa acaataaatR attcaccctcaatggttgct gttaaacttt 49141 gtctttttaa aatttaagct ttaaagatta tgactttaattattttttct tctcataaat 49201 aatatttctc ttctaaataa ttctaagtca caattatgtagggataaaaa agaaagtata 49261 cattttaatg ctttcctcca ttaaatttga atgtgtgttaattttgtatt cagtgctgca 49321 ggtttttaca gaatactgtc tttggttcca tattgaacattcaccttgag gactttagtc 49381 tattgattga aagctttagt tcatctaata gaattattatgacacacact ccaRctaaaa 49441 atcagtcaag gacagtccca gaagttaata ctcttccaaagactgatgcc tccactgagc 49501 acctctagga agtatgagat ggKgttgcat cacttctcactaagttctta taaccccaca 49561 tccaatatat caacaaattt catcagctct accttcaaaaattacctaga atccaattat 49621 gtcttattaa atccatttta ctgccaagtc acttctgatttattgcaaaa ggctcctaat 49681 tgatctccca gcttcttcac ttgcctcttc cagtctattgtcaatagtaa ctgtcaactg 49741 tStagtaaac tgtgaaaata tatctgatca tgtctctcctctgctcagag cccatctggc 49801 ttcctatctt agtcaaagta acagctgaca tcatcatagtggcctaaaga ctctagtgaa 49861 gctctgcccc atccaaatac attgcctctc agatatttactaatatStgg ctctagcttc 49921 cttggtccta gccacgctag tgtctcttct gtctcttaaacaagtcagag actcttaact 49981 gctgtattta tacttactct ttcttctgtc tgaatgctgtttctacaSag gtgctttcta 50041 attgaaggta caatcatttc tcacatatgc cttcttctcattatttgtKt aatttctctt 50101 cacagcacct atcatcacca tctgacattt tatatatatatatatatata tttccttttt 50161 tgtttgttgt ctgtcactcc cactaaaatg taggtctagaagaatagaaa tgttcgtttg 50221 ttgtttgttt tgttcactgc tgtattctta gtgtgtgcctggcattaaca ctcttttgaa 50281 tgactgaata aatatctgaa tttctgctat ttccctataagtaatattat tttggataga 50341 aatgccacta atcctgaaac attttcaagt tttatatggtttttatcttt aatatttgtg 50401 tggctttctg ctacattcca atatttaatt agctttagtttacatttatt aaatgcttct 50461 atatccagat aatgtcatat cataagtttc agttgacatttattaacatt actacttctt 50521 ataatatttt aacagaaata tgattcatca actccagtgtttgtaaatat Oatcaccatt 50581 ctgaagtcaa atgttgccaa agtcttccat taaatacttgcagacttctc tcatttggta 50641 cagccagtca ctccttacag ctgcaatatg ttatatctcgaatgtatttc ttgatagctt 50701 tctttaagat aactgcactt gcaaagttct ttgtacctagaaaactttca acaaatattt 50761 agagaatata atttactcat gcatgtggat agacacatgtaagattcact taagtgtcaa 50821 atttactaat ataatgaaaa cttggaccaa acaagcaacaaaaaatatta tccagcactc 50881 atactcaaaa cgcagtctga gagtttggta tttttcagttcctaaaggtt gatcatgtgg 50941 ttcataaaat cttagtctcc aaaatgtatt ttcaaatgaatgtcaggcag aaccaacaaa 51001 tgagaactag ggtacttagg attattgtaa ttgcctaatgtgtttggtga ccttgtattc 51061 ccacttacag caacttatgc ttctttcaaa ttcttttgtacaaaaaaaat tgtcttgtcc 51121 actctccact tagcctttaa gtcactaaga tgtggctatttcattagttg aaagcaatgt 51181 ctaaaataaa atattttatt ttaaaaatca atcatcttgcataataatag ctttaacttt 51241 atgctcaatt taaaatattt gtatttaaat aattgcttacagaatagaaa tagaaatgat 51301 atataagata atatagaaga tgtaaggatt taagaagttaggatgacagt atttttattt 51361 acttttatca tttgtaattg tatcttattg tctggtaaaaacatttaagg aaagattaaa 51421 atctaagttt aatgtattat ttataaacat ttgctccttcataaaatctt cctgcaatcc 51481 ctctttttaa aaatgttttc attgcatatc ccgatgatctgcttttcttc tctccatgaa 51541 actcagatac tagttttctt tacaacacct ggattgtttcttcctctMct gctgttcctt 51601 attttgtttt tctattctgg tgtcttgata tctccttttaacaaattcca taccattggc 51661 cttcctaact tgtgaacatt tctaatactc acatatgctctataaaaaaa agtgaggttt 51721 ttccaagaag aacagtggcc attaattctg catatatcaaaatggaatgt gtcatctttg 51781 agaaaaatat aagctcacac catgcataaa gacaactattgtttgcttca gccaggagta 51841 tgttttaggg aaaaaatatt ttaaacaagc cattaagaaatagtctcttt tttctttaat 51901 gtctttgagc tacatatgtg agcagaatta taaagaatttccagtgactc attcttagtt 51961 ttggccttcc tggcattctg tagtcgtgca tattaaaacctctaaacagc tctcagtaaa 52021 gccttggcag tagagcttgt agcttgtacc tatcaaaaatgtcagatgct tttttccaac 52081 gccatcacta gcaaacaata ttacttagtt attaacatataggaattaac ggcaaaatct 52141 gcaagttgca aacagtgttg taaatcaact aagcatttatcatgagattt tttaagtgac 52201 tgaatacaga ttcagaatca tgaagttatt atctaataacccaacaagtg atgtgaataa 52261 atccttgcta atatgaaaat tatcagccac tgaaacacccttttctacaa gcaggtctgt 52321 ggatcctcat gctaatgtac ttagattgat aaaccacagacattctctga aagcttcgtt 52381 tgttccagtt gctgaggctt gtgcttagag catctgtcatgcaaatgatc agcaaatcat 52441 aacctgtaat ttgttttcat ttaataggca gacaggttttcaccaattta gactcaagtc 52501 ctattaatac tctttgaaac taattgctgg aaaatgaattttaatgacag gattttatca 52561 tttattattt tcccttctgt ccctataaag gaagactcaaagccatctac tcacactttg 52621 aaaatatatg ttaaataggc acaagccata atctcctgtcatctgggctg ttgcaaagga 52681 cccctaatcc cctctcttgt aataatccat tctacactctactcttaaaa ttactttctt 52741 actatcccat ttcccttcca caaaatcttc aattccctgtcaagtaaata gcagacactt 52801 taatatggct tcgttgccct ccattatctg tttgcagtttacatattttt atccttattt 52861 gatatcgcaa tataacatgt atacagaaaa aagcatgaMacattaattaa taagtagtaa 52921 agtaacatcc agaaaacact actcagagca aaaagcagacgattgtcatt accacagaaa 52981 ccacctcccc agtgcatctt tctgatcaga cccttcccagcatcccagag tctaaccacc 53041 cagagataat cacttcctct attttcttta gattgtctccagctacgtgt gaattcttaa 53101 ataatatggt caatttaccc atttttgaat gttatgtaaatggaatcaaa atctgctttc 53161 tttggctatt gcttttttca ctcaaattat tctcttaaacattcattcat tttagggtac 53221 agcttcagtt aacttcaatt ttaagctgtt tagtattccactgcatcaat gtaccacaat 53281 atatttatct tttccatgtt gatgggcact tggatttttagattgtttcc acatttgagc 53341 tattacagac aagggtgcta tgagcattct tggatgtatagtttggtgcc cagtattctt 53401 ttgtattcat acctaaggag tggaatttct aggacttagccatgcatatt catactagat 53461 aaatattgat gtaatacgta aaaccaagta atattaacttgtatataagt agctctgtat 53521 gctactagac aagtcaaaat atttttcaaa atggttgtaaatttatatat cgagcaggag 53581 tgaatgctat tcactcctac ttctatttcg acttctagaaataggagcac ttctatttct 53641 ttatatgctt gacagtactt gggattatca gactacctcactttctgttc ctcctctatt 53701 tattcttgct ttaggatttt ctatcctctt gtttattcttccttcaccct ggatattaat 53761 tctgacctat tgcaattcat gaattctttc ttcatctgtttctaatctat taatgactca 53821 gtcatatgtt aactttggta attgtctttt ctaattctcaaatttgttta gttctttttc 53881 aactgcgtaa caacactcct ttgtagtttc cagtttcctgccaattaaaa aagcattgcc 53941 cttcatttct tcaaacacaa taaacataat atttccaatatgcatctgaa aattctagta 54001 tctaaattct tgaggatcaa tttctgttcc ttgtttcttctgcaggttct attattgaag 54061 aattattatg ttggtgcaaa agtaactKag gtttctaccattacttttaa tggcaaacac 54121 cacaattact tttacactaa tctaatattt cttttcaccacatttctttg atttttgttt 54181 ggacatcata ttcaaaaatt atttgaagga atcatgttacacctaaagta atattacctt 54241 tttctagaga ggattgtgtt tttctctgtc aggaatctggagcctccatc agggtaccac 54301 cataaattgt gttaaaattt gagattaaac tatgcagtcctggattttaa tccataggtg 54361 gaaaaaaata gagtttcaaa gaggtaagct tcaattaagacagattatga tgcctccctc 54421 ttctgtaaca tattgtgtga ctttgagttg agattcttcctaagttttct gatctattaa 54481 aaaagatata gctggtaatc cccaaaatca tgattttccttttttttttt tttttttttt 54541 tacagtttct actgtatttc tttgttgtcc tgtcagtaccagttatggtg tgtgttgctt 54601 tttctttctt tatatttttg ttgagcacag tgattgagtacatgccccac taatagggta 54661 taattatttt gagagtaata tctatgactt aacaccatatctctcactat attgagtgta 54721 gtaccttgat gcagtagcta gctgctcagt gaatgttaaatggataaatc aatgactgaa 54781 taaattttag agataggtac atagataaaa gtgatcaaaacaagctaagt attactgcag 54841 agaaggaaca agtatctttt ctattatagt tcccaaagcttcaaccaaat ttcttttgct 54901 atgtttacct gtccaatata ttggctggtt gtactcatagagtactagtc ttagttagaa 54961 tttatgagta atggtccaaa tccgtaatag agcttaaaactaaagcttta aaaacattga 55021 tccagatgaa gaatgatagg atgcaatgaa agtttatttagggcataaaa tttaaaaggt 55081 atgttatcaa taattcattt tgaaagtccc cttaatatagccatttctat tctgtatctt 55141 taaccagtta taggtgacat tgctaggaac agattttgacagcagacttg tgcaagggat 55201 ctgtttgcct agagatttac ttaaaaggta gaattaaagtcatttcaaga aagaaattta 55261 acataagaaa atataaccat ttagtacact aaataattggtctagctagt tatgagttaa 55321 aaatgaaaaa gaattaatag tttttagcaa catatacattgggacagtat ttttgccaca 55381 tctatcattt acctatgcaa taattaaatt cataattttttaagtgtcat atcgaagagt 55441 gttattcaac ttcctggtga ttaaattact gataaacatagccgattttt tttggttttg 55501 caggtcctga aaaatagaga atgcatatga cttccaaatattggtagaag agccagtcaa 55561 ataatatctt ccatgaatcc tcttacaatt aatttgctgattgatagatg ttcaaaatct 55621 agaaatgaat gagttgcatg taaggaaatc agtacatgcttctgctgcca tcatatatca 55681 caatacaagc attaaaagct atattaatta tagaactttatgatcttatt tacatggtct 55741 gtgaatttga tctttatttt ggaattcctt taatccattctgattctaat cacctcagga 55801 ccaactttag tgtatttgta caaggcgtaa tcattggctagctctataag ttgtccaaac 55861 aaccaaatga gatgtcgcaa attctgccaa ttgtgtaaagttttattatg tactttaaat 55921 atatgaatat ttggaatatc aagatgtgtt gtattttaccaataatatta aaggtttgct 55981 tgttcacttt tcacttgtat tatgatgtca tttaaacaagtcagtattat tagaaatctg 56041 attcattctg aaaacctctc atctactgat ctaatgatcttcaatattca ttaacatgaa 56101 atattactat aataggaaaa aaatcggtac cttaacaaaaaatatggaaa tttatttgtt 56161 tatttattat ttttacttat ttatttattt atttatttatttatttattt atgtttttga 56221 gacagtctca ctctgttgcc caggctggag tgcaatggtgtgatcttggc tcactgcaag 56281 ctctgcctcc caggttcacg ccattctccg gctgcagcctcccaagtagc tgggactaca 56341 ggcgcctgcc accacaccca gctacttttt tgtatttttagtagagacgg ggtttcacca 56401 tgttagccag gatggtctca atctcctgac ctcgtgatccgcccacttcg gtctcccaaa 56461 gtgctgggat atttctttct ttctttctta cattctagagccttggcgag ttggcccaag 56521 tgttgtacag cattttcRgt gtcagggaca tagacttctctcctttctct gctgtgtgta 56581 gtctcagata gttaccagag caccagccat tgtatgttctttctagtttt caggtagcag 56641 aaagaataag aaatgttgca caaaccaccc tgtttacatcacgttggcca gaattagtca 56701 catggccatg aagcaagcca ctttgcctga agtaggactacagattgtct ttattctcca 56761 tagtggtaaa atataaaaaa atataattag agaagaagagaactcctctg gaggacaact 56821 agcagtaacc tactctacta actgtccatt tactagttcattttgctcta agataacata 56881 taaacagtga aactaaaata tgttttcaaa attagaaaatgtaagtacct tagataacca 56941 agattaaaga aatgttacaa ataccacaat atcaacatagttatagatga acatcaaact 57001 tagtactgat tttctgaata tccaaaatga aaagaaatacctcaataatt ggttacattg 57061 ttttttatca ttttgaaaaa ggcactactg aggcaaatgaagctctttga ccctgaacaa 57121 ttacaaaatt tatctcatag caatttttag ggagaaaattgtatattata gttgacaata 57181 ttcctataac aggtgcagtg attttataag ctacttacagtgtctttgaa taaaagccaa 57241 aatgaaagca ttatgaaggc tattttgtga atctctgaggaaaaatcatt caaaaaaaaa 57301 aaaaaacctt tggctattct ggcttatata caaggagaaattttagcctg cccagagggc 57361 tggatggact acatacacca tgaatggtta tctgtaaatattactgttgc tgaaagattt 57421 ctgaacaaat gtagaaaatt aaggaataga ttatctagttttctgtagtc tgccagcatg 57481 tttcaaatca atcgagcttt cttaacacca ggtgttctcagaagtgaaag gggcctattt 57541 gttagcttta aaaagacaaa aatgggaaaa gataaaagaggtggaagcag aaaccaattt 57601 gctaaattta ctaaattaga cagtttattg aaaaggtaagtgaatttctc atatcagtca 57661 taaagaagct atttttccaa tgaaatgtgt gtttatatcaaagtcacaaa attacttagt 57721 gaataactcc cagtaaatgc gatattatta atttcagctaaattatatgt tcttagaatt 57781 tttttatctt ttcagattgt tttatgtaga ttatagctgttaaaatttta ttggatgtaa 57841 tttacatggt ttacaaacaa taaattcctg agaatagatacgataaattt atagtttgtt 57901 gaatgattta ttattaagga gtctcagatt ggtcctccgaggacaggaaa aataaattca 57961 tggaccctaa gttttttagt tacttttatc taatttttgatatggtaata attaaaaggg 58021 agatgataag aaattgaatg attaagcatt gttaaataatatgatgcgtt aataatgcat 58081 aagttatatt cctgagttga ttctttcaaa gtataaaacaatttttagat ttaatcaaaa 58141 ctctttaaag aattaatctt tgcttttagg gagcactatagattgacatc agaaatttct 58201 cttatttcca tgtaggaaga accttttgtg atggtctctgaaaatgtctt gggtaagccg 58261 aagaaatacc agggcttctc cattgatgtt ttggatgccttatctaacta cctgggtttt 58321 aactacgaaa tttacgtagc accggatcac aaatacggaagcccacaaga agatgggaca 58381 tggaatggct tggtaggaga acttgtcttt aaggtaagaattactttatt tatttgtctc 58441 atacttaaag gttcaattat ttgtctcata cctaaaggttcaacagtaac accttgaagc 58501 tgatttcata taaaataagt gtgatgtatt taggttggttaagcattagg tggtggaatt 58561 gagtacaaaa gtctcactca atactatttt atgaactttgtggaaagaaa agtagttgac 58621 aaaataaacc taaatgttaa aatatatcct gtaatagaaaagggtttaga agaagacctt 58681 ttctttgctc aacttatacc ccaaaaacag aaagtgtatatgatacgtga tgaaatcaca 58801 tttttttttt gagacagagt ctcactctgt cctccaggctggagtgcagt ggctggatct 58861 cggctcactg caagctccgc ctcctaggtt cgcgccattctcctgcctca gcctcccgag 58921 tagctggact acaggcgccc gcccccacgc ccagctaatttttttgtatt tttagtagag 58981 acggggtttc accatgttag ccaggatgga ctattgttttctttttaaaa atgtgatgtt 59041 attggaaaaa attaaaacat tcactgctta Raaactaatcatttcattct gtaatctaat 59101 caagataggK atgtacatat gtatatattt ttctttgttctttcaattct cagaaaggtg 59161 tttaaaacct ctatgtcYtt ctttagtcct gtcagaatctgcttcagttt tttgaggctc 59221 tgttattagg cacatagaca tttataatta ttacattttcctgataaatt attgaatagt 59281 tgggtttagg tttataactt actgtttggt ttatatgtataccatttttt tctctgttgc 59341 ttcttttcct gtttttcttg gggttaatta aatatatttcagtatttgat tttattgctc 59401 ttactgcctt tttagttgtc tccctttgta tttttgttcgtggttagtct aaaggtcata 59461 atatgccttc ttaacttact acattctatc ttaagttaatattgtactac ttcacataaa 59521 aagtaataaa ttttcagcaa tataatttta tttgtactcactcctttttg atattgtgtt 59581 cacatattta tattttatta acctcatacc aaatatcatagttttttatt taaatattta 59641 ttgtctttta aaggtacaga aagaaaaaaa aaaagacagttgctgggata taatcactta 59701 tctaccgtgt ctggggctct ttattcctta catatatttgagctcccatt tggtataatt 59761 taccttcaac ctgaagaact ttctttagag tgtcttgaagtgcagggcta cgtaggtagc 59821 atatactctc aacttttgtt taccttgaaa tgtcttttttaaccctcatt tcaaaatgat 59881 attcttactg gatatcaaac tctgaattta cagggttttttaatgttcat cattttaaag 59941 ttgtgattct atcagttttg ccatatatta tttctgatgagaaattagcc atcatctgta 60001 tcctgattct ttatatgtag tatatttttg tatgtgttagtggccattct acaagtttct 60061 cttcattttt tggtgtttag aaatttaatt atgatgttctagatatgatt ttctttgcat 60121 ttattgtgct gagagttcac taaacttctg ggataggttacatttagtaa atatagagaa 60181 ttatttcttc aatttttttt accttctttc ctttctcttcttgatttgag gctcagagaa 60241 tattcaagtt agatcactta atgttgtctc ctaagtcactgaggccttgc ttgttttatt 60301 aagatatttt tactccctga ccttcagatt tgacaatttgtcttgatctg ccttcaagtt 60361 tactgatatt ctgccacctt caaatttttg ttaagtcctttcagtagctt tttcattcta 60421 gattgcttat tttttctttc cagaatttca attttttgaaataattttta cttttctgct 60481 gagatttcct atctttttat cagttatgac tttcttttcttaaaagttca tgatcacatt 60541 tataatggct tctttaaagt ccttgtttgc taaaatttcaacatcttatc atctcagaat 60601 acattgcatt ttcctggttc ctcacatttc tggttacatttgattatata ttggacttcg 60661 tggttgatac attgttaaga cttgggatta tgtctctgaaattgtatatt ctgaaaagga 60721 ttgatttttg cttcagtgga tagttaaatc actatctgttctcttgaatt tgtggagatt 60781 tagctttaaa cattgttaag gcaggtctat ttgatgtaatcttggtccct agggcaaatc 60841 ccttggtttt cgtagtccac atatttctaa gatataaccttctggagttt ctggggaaag 60901 cctgtggttc tcctcaagcc cttctgactt aaaggaactcaaaattcaat ctgtatcttc 60961 cctgtcatag gcaacagttg taatcccctc tttagcacgtttagccttct agccattact 61021 ctttgctaat tttcctagga tctctcccat atatgtgcagtttaggggtc agctaaggat 61081 tttagaRagt ttatacacag attttggggc tcactcctctgtggcatcct ccttcttgtg 61141 tttccctttt caatttctgg caaccctagc tgattcaaactccatcctct gacaagttag 61201 ttaataagac tgactttcta cttgagctct gtcgaccccatccccgctga actgaagtgg 61261 gtcctcaatg actaagccta ataaaggagg gctctacccSgtgcagttat tattttcaag 61321 ggtaccctgc agtttctgcc tgctttgggt tgctctccagtcttttctat atattttttt 61381 caatttgatc ctgaattttt aatttttatt ctgatagggctagtccaata aaagctagta 61441 catcattgct agaattggaa taatttctca aatgctttaaagtactcaga aggctagagg 61501 atctaaccta aattgtttgt aaggaatgga gaagagtagaaataaaaggt ttcttaaggc 61561 agtggtaggg aaaataataa agctatttct atattctataaaatcctaat gaatttggtc 61621 tatttaatac ataagttaca tatagaattg tattggaaagcattaatatc gttattttta 61681 atacaaaatg ggcaatcgtc agtcctcttc cttcttattaaaaccgtgcc ccttcatctt 61741 ctattctttt aaaagtcaca aattcagtgt tccacctttatctttctaag ggctgtttcc 61801 tgatctgaac ccagattagc aattacgtta aaattagtgtaaagaaaagg cattatacaa 61861 atttattcta tatagatagt ggttaaaatc cttaatgagttattatgcta ataaactaca 61921 cttgctagat gcaaattaaa cataaaaagc attcaaattagtatgttgat gttgttttta 61981 atttgtttta tgtgcttaca ctagttagtt taattttggagacttacttt ttcacctata 62041 aaaatgagga gtccaagtat gttaatggta agatcacttccaaatactat gttttgaaaa 62101 tataagttta tcgtatagca atcatacaca agaaatagaatgttcttcat aaacaatcaa 62161 tttcaatgta Ytatttgcat gtaagagcat gtcattattgtatgataatt tatagtttag 62221 agggagtcct tttgcaaata aaaaattgag gcttaaagaagcagacatat gatttttaga 62281 cagaaaaaat ataaaattat tcattggaac taggtttttattttgttcaa ccggatattt 62341 agtcactctg actaggcttc aggaaaacta ttaagcaggtggaggagaag aaacctacaa 62401 aaaagactta gttggagtag atagagagat taaaagaaaattgagaaaat gggttgtcat 62461 gaatgccaac agaaaataac atttcaagaa ggaaagagcagtcaacatta gaaaatgact 62521 atcaagttaa gggtacacaa cattccctta actgtattctgactgttatc agtagagatg 62581 taagttataa tttttaacaa ttacaaatgt taattcaattacataatgat actttaataa 62641 aataccataa aataatttag ctttccaaga aaattcatcatattcaaact ttatgaggtg 62701 tatttacccc tataaaccaa aattacaccg ttgaaatggcataaaaatgt tatgatggag 62761 ctccagaaaa gcaagcttat taWtgttatt atatcttctgataatataca tacttttgtg 62821 aatctaagta tattttaaat aaatgttgcc actaaatatctctgtacaag acagtaagaa 62881 ttttaaaatt aacaggatta actcgtgtgc ctttctaaggggctaccaaa cttaaaagaa 62941 tgtttcacat agtgcaatta tatatatcat aatgattatgcaYgtaccat cacattaaag 63001 aagagtcaaa gaagaaatgt tggaaagtga gctctgaattcatatgatta ttccacagtg 63061 attagatctc atgttagaga gctttctcat taaaaccagaagtcagtggt ccagtacata 63121 catactttta agagaggaaa atgcagtcag agagagagagaaaatgattg atatgatgta 63181 catatggagt ctatttaaaa actaataata tagtaaagtctcctcaagtc cttctctgcc 63241 tcactactct tataattttc tgcaattaat gtgttcttaaaatttatgaa aacacaaatc 63301 tggccaaaag atatgaaaca gataaatgag agatatcttctgcctagtac aggagaaata 63361 tttaagggtg agttaatgta actgagaaat tattttctcctagtttagaa gggaagagag 63421 gcccacagag aactttcaat aagaaataaa aagtatatcaagtttaggat agaagtacca 63481 aaaaaattaa aaacaaagat aactctgaat tagatacagttggatgtttg atgatatagt 63541 ttcttctttg gctggctttc aagtcagaag cttttaaagtggagtattta ggggtattag 63601 aagactttcc aagggcaagc cagtatattc agcacagaaaaatttcaagg tgttgagtga 63661 cattttgaaa tagaaactaa aaacttcctt tttcatatattaatttgttt ggaaaagatt 63721 tctataaagt aaaaaaatac agaaattaaa tcttaatgctaaactctacc tcatttaaac 63781 cataattagt actgacccat ggatatctaa attatttttttaaaaggctc tatccataac 63841 attgtaagct gcatttgtag taaaacttca tttttatagacactgaatcc atagatcgta 63901 tactaatYga atcatgactc aattcagaaa aaaagattttatacctaaaa ctttatagtc 63961 actggttatt taaaaaggat tgtaatttta atttgtatgcatatttttga tgcaaaaata 64021 ctttggtgat caagaaaaga ctttcaaaca cacatttctattgtacttga attacaattg 64081 tgtgaggKtg gggcggaata taaatatagt tcaagcataaaataaaatga tataaatatt 64141 ctacctatta aagcatgagg tcaaatcact atgatatttagattctaaca gatatatttt 64201 taaaagtgac ttgacaatat tattttaagt tatcaatatttacaacacaa tatacatatg 64261 tcagtgccga cgcccactca ggaaaatgaa aaccattcctaaaattttga aaagaggaga 64321 atttaatatt gtgggttgat cacaaaggcg ctagaaggaatcaaagagca aaggatgaac 64381 agagtaggac attggaaaag caaaaagaaa aagtaaaacacagatatcaa gtgttactga 64441 tgtgtcagtt gattgtatct gccagacgta tgccccttgatcagacctgg aattgccaaa 64501 aagttgctgc ccgtgcccta gctgctggga tcaagaatcgcctactgcca ctgctgctgc 64561 agccactgaa ataaccattt aaagcagacg gctcttctctttctttttct gtctgatatc 64621 ttcagtggtt tccctcaggt ggaaattatg gagaagccagatggcaaagg agcctgggaa 64681 atctggtttg cagattccca tccctataag gctcacagtatatagcagag tagaaaatgg 64741 tatgtatagg tttaagagaa aacaggtaat gatcagtactgtctgcaaat tgcgcccttt 64801 acaattttct aaatgattgc aaaattaata tgtcaattataaatgtagaa agatacatac 64861 ttaaaaaatt cctttggggt ttatatgaag aaaaaagcttgatggttatt acaatggaag 64921 atttatttta tataaagaac Wtgactggtc gttttggatttaaaaataat ctgttccttc 64981 agcctaattt tacctgagcg tttccaccac ctggaaattaaatctcttca tgctaactga 65041 ttatcaaatR ctaaattata tgacaagaat tttactccccaaggaaaata gatccaaaaa 65101 tggaagaaag ccagaaccca ggaaaatgga aaaaaaggaggaataagtgg actaaattat 65161 aaatctgtaa aattaggata aagtactata cttagcatgtaagtattaat agtgatgttt 65221 ccatgttaaa atatagcacc attgagagtc ctatctctcaatttctaaac ttctagtata 65281 acactaggct tttcttgtca ctgtgcccac ccacgtggtttcatgagagt aaagaatgtt 65341 tttgaaagta gacatataat attggagtat aataagatactatttagtaa ttataatgcc 65401 aataatgtca ttttctgaga aagaaaatgc tcccaagactaaacagagta aatcagctgc 65461 ttgggactac acagctttcc taatttttat ggttgacacaaagtttggca attctttagt 65521 tttactaggt aaatgaattc ctatgatggg tattttagttcatcaatgca ttaacaatag 65581 aaagcatata tttaccggga ggctttataa atattttattttattatttt atttatttat 65641 ttagagatgg agtcccactc tgttgcccag gctggagttcagtggcgcaa tctcggcttg 65701 ctgcaacctc cgcctcctgg gttcaagcga ctctcctgcctcagcctccc aagtagctgt 65761 gattacacgc acccaccacc atacccggct aatttttgtatttttagtag agacagagtt 65821 tcgccatgtt ggccaggatg gtcctgaact cctgacctcaggtgatctgc ccgcctcagc 65881 atcctaaact gctagaatta ctggcatgag ccactgcgccctgcctataa atatttcaaa 65941 acatatgcca cttaacatgt gttaatcaca tgccatggtcaaagtgctat ggagggatgc 66001 aagtgtgaat aagacataga cattgatcat gaaaacattttgttgatggt ctctatgtgc 66061 tatgcactgt gctaggcact ggagatacaa agataaataaaacatggttc ttgcccttga 66121 agagatcttg tcttaatgag aaacacagag gttaaactagacacttataa tacaatgcac 66181 taggtgcaat gatagagaag cactaagaaa gcaacaaggaaggtagagta aaggtaaatt 66241 tcctgaagga ggagaaatat agcctgatga caaaataacaggtagtaatt atctaggtaa 66301 agagggattg gcagaaagtg aaggacgtct actgacagacacaagagtag aagtaaaggt 66361 agaaaggtta gaaacagcat ggaaagcagg ccaaaatttatgagaatttt gcagctctcc 66421 aagcaaaaag tgatgagaat ctatcctacc atactaatgttgtgaagaga atgaaagaca 66481 ggaaatcaga gataaaaagg aggtaggata aacagaatttattggctaaa gtgaggagaa 66541 gggagaagtc tggaataatt catcagttca tagtacaaatacataaaagg gtggttgtgt 66601 aactaattgg gacagagatt gctgaaaaag atttagaggaggaggacagt ttagaatagg 66661 gtgagttgga tatgtagcat tctgaagctc actgtgaaagtgccatagga ggtatacatt 66721 tgggagtcag caacctgtgg gtggtagttg aaatcatgaaagtacatcag attgccaaaa 66781 agaacacatg gagtaagagg atcactgggc taatcttaggatgaaaccca gagaaagctg 66841 tcacttaaga attgacaaaa taagaagaat ttatgaaaaaaaataagaaa gtttcaaaaa 66901 ataagctatg actagcaatg tcagaactat ttgagatgttgatgataaga actgccaaat 66961 gttcactaga ttggccattt gggagttgtt ggttacccaagccagaactc ttttggtagc 67021 atagtgggag aaaaagccag actgcaaaag atgatggaaaaacttggatg tgaaaaggtg 67081 aaaatgacat gcatatgtat tattttttca gaaattttagatggagaggg gaagaaagag 67141 ataataattg gaagggtcat tggttttggt agagtttttgtttgttttta agttaaaaca 67201 gagacttgag taaaattata agctaaggaa gaaaaagaaagaaggaaaat gtaatataag 67261 aaagtcagtt aatatgtgaa ggataaatat cctggaacagattcaactcc atcaaaaatg 67321 attaaactga aaattgattt taatgaggta aagatgttactttgatactg aatataatat 67381 aatgagaatg tgtatgttaa taatagatag gcaagagaatggaaaattga caaagatact 67441 ttcttattcc ttcaaattta ttgataaaaa ttggagattacgtatgttga cagtgatggg 67501 gacatgtatt atgtaacagt ctacaggaga gtggtgaagattgaaataga cattgaggaa 67561 agggaaagtg actgctaaat cagaacagat aaaagaatagatcagtggca ctaaagactg 67621 agtggaggtt tatatgaaac tgacactttt ttattgctgctagtttacac aacagtgtga 67681 ctttcttcag ctgagattga gtgcccaagt ctaggagcagagatgaaaat gttcatatca 67741 aggttgagac atggtggata aacttggtaa gggaattaaatgagatgaaa gaagagctaa 67801 aatgattaag gaagcatagc catgatcaga gacataaacttggataaaat tttggaaatg 67861 ttaattagaa gatcaaatga agttgaaaaa caggtgaagtatgagtgaag aaatgaaaga 67921 gtaggttaga aagacataac cagagactga aatacgaaagttctagaatt tgaccttgag 67981 gttcatggca gagtgttaga gaaaacaaaa gaaatgagtttaaaatagct caaaatggag 68041 tgagggaggg agggatgaat agatgaagca taggggattttcagggcaat aaaactattt 68101 tggatgatac tctaatgatg gatacactgc tgacattatacacttgtcaa aacccataga 68161 aagaatgaac tctaatgtaa actgtacacc ctagttaataatcatgtatt gatattggtt 68221 cattggccag gcaaggtggc tcatgcctgt aatcccaacactttgggagg ccaagatggg 68281 aggatccctt gaggccagga gtttgacacc aatctgagcaacatagagag acctccacct 68341 ctaaaaaaat aaacattaaa aaaaattagc tagacatggtggtgcatggc tctagtccga 68401 gctgttgagg aggctgaggt gggaggatcc ctttagctcaagagtttgag gctgcagtga 68461 gctgtgatag caccaccgca ctctagcctg gatgacagagtgagatccgt ctccaataat 68521 gattgtaagg ttcattaact gtaacaaatg tacctcaccaatgcaacata gtaataatag 68581 ggtaaattat tcacactttc tgctcaattt ttctgaaaacctaaaactgc tttagaaaat 68641 aatatctatc tgaatcatga cccaaaatga ttgttgtaaggaatatctac attgattctg 68701 aaatatatta ggaggttggt cagtttttgt gtgaagagaaacatttagcc aggtctcaaa 68761 gttttcagag aaggagaaga agatgctatt gtgaggtttgtctggtggag gtgatgagac 68821 ctcagaaagc acatgtaatt gacaacataa aacaaaacaaaacaagaatg gagatagcat 68881 gaatagagtc tcattaataa ataacaaaaa acaggttccttgagtatcta agaaaggcaa 68941 aattccatgg ccYctaataa atagtgaaaa tgatggaagagaattcccac gccatgtcag 69001 ctcaaaacaa gtatattttt tgcccaagcc atgctcaagctcttgctctt tttctatgcc 69061 tcctgtgctc atccccactc ctattgtcta ggatcccgcacactgggtta ttgatgtagc 69121 tagtcatgac acttggctga tggagttcat ttcaataccctagtagctaa tgtcaataga 69181 actttcattg attttccagt gtcctttaat tcgttaggtattgacctttt atttaaatcc 69241 cacatgacca ttccaaacat tttccacact tttcaagcctcaatttctag tcccttcatt 69301 ttttacaccc tcgtcatgtt cagttgttaa actctatggtttactgcaaa agctgtgaga 69361 atctagcgta agtcccttgt gttcttgata ttcaacctcaaaatccttcc atgattctct 69421 catctttagt gacatcaaag gcattagtta tggatactgttgacacattt aatacccatg 69481 tgagcctaaa agagttttga atcctctctg agtcacaatgtccacatcag tggaatgaag 69541 ctattgtgaa gatgaaccaa gaaaatgaat tcaagaatgtgttgcaggaa aaacaaatag 69601 atcttgttgt atttgttgac tcggatagat tggtaaaacttcctcaggtt ctgtgtttta 69661 aaaatgtaac agatccaaca catatttgga aggtagtctgataggatttg ctgtggggaa 69721 gtgagagaaa gaccaaagcc gagaataatg gtttccagcttcatccatgt ccctaaggag 69781 atatacctaa tgtaaatgtc gagttaatgg ttgaagcacaccaacatggc acatgtatac 69841 atatgtaaca aacctgcaca ttgtgcacat gtaccctagaacttaaagta taataaaaat 69901 atataaaaaa aatttaaaaa agaccaaagc caacaatctcttctaaactc atcccttttt 69961 aattttgtct tttcagccac agcagcagaa aacaaaaacaaaaacaaaaa aaaacagcct 70021 ttatttccat tttccccagc tccactcact atcttgttcctagccacaat tatctcttct 70081 ctggattgta gcYgtagagg tccaattact ctccctttccactcttgact gccttcaata 70141 tattttaaca cagcagccag agtgatccag tcattttcctgctagaagac ttttgatggg 70201 tttccatccc actcagagta aaaactgtcc ttaaaattgtctacataatc tgcccaatca 70261 attccctttg tgatctcttt atctctccct ctgtcaccatgctccaagct tatggtaccc 70321 cttgtacttc cttatactca ttgaacttgg ccttttcaatttctgtctct cctatctgcc 70381 taaaatgata tttctctgtc cacctcattt ttttttcagcatttgcttaa agcttattag 70441 agaggactgc cattgccacc ctcttcaaaa gaacaacttccatcctttgt actacctatc 70501 tctcttattc tgttttattg ttttttataa cacttacaattattttatta tgtactgact 70561 tgttcatttg tcttcctctg gtaaaatgta agctgaagttggtctgagat ttcatatatt 70621 tggtttacta ctgaagcctc aatatctaga aaatatttgtacataaaaga atgaatggtg 70681 gccacatggc atacattagg taataacact cccctaattaataaccattc aatgggatct 70741 cattgctctt taataaaatc aattaaccta ctatagcctactaaatttgg actttatata 70801 aaatgttttt atctttgttg aaagactaca ctttgagcttttgttcctat tattttaaat 70861 attttgtcca ttaattaaac agcttgttat attcagctactctcacttta cttcaagtta 70921 attgagaatg gtaccattta ttggcacaga agaaatgaatgaaggaaata tttggccagg 70981 agttgcagaa tgcggaatca aaactactat tttagaaatcagtaataata cccagatgct 71041 attctaaagc tttacaggta ttgactcctt acatgctcttactaatttga tgaaatagat 71101 agttaatatg cccattatgt agatttaaga catcaaggcttagagaattt aaataccttg 71161 ctcaaggtca catagccaat aactggaaga accaggatattagcttaaat tgtttgattc 71221 cattctctta acaactatgg atatacctct ttggagctatccaaatggaa aatgaaataa 71281 gtaatctgat atatgagtct ggagttcKgg taaggatatggaatggacac atgcatttga 71341 ggccagtctg catatagatg aagtttaaat tgcccctctacctttcttta tccctacttc 71401 tcttttattt tcagtctctt tcaaattctt tgctccttccccatttctta aaaaaggtat 71461 tctctatctt gaaaataacc tgctcatgta ttctaagctaaattaattag gtgtctttgg 71521 ttgcaaatga caatccaatt gaagtgaaaa gataatttaattctcacaaa actggcctat 71581 tcagggatga gatccaggct cctttcatca ggctttggtatcgcttgctc catctctcag 71641 ctgtgatttt tttgtgtgtg tgttgcccca gttctcaggcaggctctcct ctcttggcag 71701 taaagtgact tttagtagct cttaggctta gatttcatcctcttatctat cccaacataa 71761 acatagattc tcttttacct gcagttccaa tacagagcctcagctttaac attcactggc 71821 tctgactatg aagtattgtg ttcaagagga ggcagtgttctcaatgccag gcctacacca 71881 cttgcccagg tggagcactg agagtagggg tagctacacgcaaaacacct agagcaagcc 71941 tcggggagta gtgaatccca aggaaaagta ggatgctgttatcagaggaa ggggaagtag 72001 ataccaaaca ggaaaaacaa aagatatcct tcaggggcttaaaaacaata gaataagtta 72061 caggaaacta tcaatagtta catgtacaca aaaatttaaatcgacagtaa tacaaaatca 72121 taatgaacta aattaaacta caaacaaaat gctagaaaatatttgtaaaa gtgtaatggt 72181 gacttgtttg tgtcaccgct tcactgctac atgaagtaaaaatcaacaag aaaatactaa 72241 gaccccactg gatagatata aaagatatga acttagagtttcaaaaggag aaactagtag 72301 gcagtataaa tccgaaagca aatatattgc aatggagaagggcagttaat cattttttag 72361 aaagttaagt ttatgaaata tgattgatat atattttattttacaaaata atgtaaatgt 72421 gttatatatg cttggaatta ttttatatgt tgtatttcataataaagtag aacatcacca 72481 gaatttgatg acatacttgt catcaataat agatttgacaacactgcaat taacacaaaa 72541 cccacaagga atttaaaagc atagcttgaa actgaccaaataacaactat actagcactg 72601 atatagagct tacttattcc tgacacagtc tcacatactatgtatttatt acctctcagt 72661 actcacaatg ataagtacta ctattatctc aatgttgtagatgatgaaac agagagaggt 72721 gaagtaactt gcccatagtc acatagccaa gttgtagagaattaattatt caaattcagg 72781 aagtttggct ccagcatcca agtgcttaac tcctttaccagtttccagag aaaaacagtt 72841 ttgtctgcaa agcatttaaa taaacccagt tacatgtcagtgagaaaaac aatactcaaa 72901 cagttcagag ctgttttttt ttaaacctgc atatttgaagaaggttcaga taggagttca 72961 gacagagtat gagtagaaga caaaacataa gattacatagagaaagatWt ggggtgagat 73021 gtaaaagatt tgcatttcta aacttctcga aattcacacttctttctcaa acacctgtgt 73081 cttctttcca taaaaaatta aaacttttag aaaggactgcaatatactgt acagtctata 73141 tctcacttcc ctcttgcatt taatcacact ccatttagatgacattcagc tttaatctgc 73201 ctagactgcc tgtactcact tcatttctca accctagctaaacttgcact ttgtcccata 73261 aggctttcaa actactccca tctcttagta atctcgtcctctgagaacac ctattccact 73321 tcttttcaaa ttacctttgt gtaatgttac acatctatctatatatatct acatctatat 73381 ctataaatgg gcatatatac aatctcagga agagagaacgagagagatgg agggagagag 73441 agaccttctt tttgcatagt ccctgttttg gaaatcagaagactaaggtt atgtttactt 73501 tgtatgccaa gcacacagca gaatgcccta aattcatccatttatctgca catgtttttg 73561 aatgcatgtt gtattctggg cactgtgcct ggcatcatagatacaaagga gtggaagata 73621 cttttcctcg gccttcaaag atctctaagt gcacacgaaaaacaaatgtg tagataaata 73681 attaaaacac agtgtgaaaa atgccatagg gtaatacatacagggtgcca tggcaaccca 73741 ggggccgaat ggcagcttct gcctggggag ttgaaaagatttcacagaag aggagaaatt 73801 taagctaggt cttcagatga gtaggagtat aagaggcaacaatagggata attataaaag 73861 catgtttgcc ttgataaata gtttgcactt tatcatttagccagtacaaa ttataaaaga 73921 gttttacaaa agagaaaaat gtgatttaac ttacgtttaagaaagatagc tgaggtaggc 73981 ctgagaatgc attggagtga gRaagagaaa aaaacaaatggctttttcaa caatctaggg 74041 aggaataatg gaatgtctac attaaaacaa cagtaacaggagactaacaa aaagaatgag 74101 tctgaggaaa gttaatattc tttattttat tttattattattatacttta agttttaggg 74161 tacatgtgca caatgtgcaa gtttgttaca tatgtatacatgtgccatgt tggtgtgctg 74221 cacccattaa cttgtcattt agcattaggt atatctcctaatgctatccc tccccccatc 74281 ccccacccca caaYagtccc cggagtgtga tgttccccttcccgtgtcca tgtgttctca 74341 ttgttcaatt cccacctatg agtgagaata tgcggtgtttggttttttgt ccttgtgata 74401 gtttgctgag aatgatggtt tccactttca ttcatgtccctacaaaggac atgaattcat 74461 cattttttat ggctgcatag tattccatgg tgtatatgtgccacattttc ttaatccagt 74521 ctatcgttgt tggacatttg ggttggttcc aagtctttgctattgtgaag agtgcagcta 74581 taaacatatg tgtgcatgtg tctttatagc agcatgatttacaatccttt gggtatatac 74641 ccagtaatgt gatggctggg tcaaatggta tttctagttctagatccctg aggaatcgcc 74701 acactgactt ccacattcta tggcataata atagaaattgataacttgga ggagaaaaga 74761 aggaagggtt gagacttggt gagaaattta gcagaggaaaatgagcagat ggacactcca 74821 caacaaaaat aggggatgca gaaggaaaag cagattggaaaagggcaaca gggacacaSa 74881 atttggctat aggcattttg attttgtggt acctgaggaacattctcatg gaattgtcta 74941 ggaacaatta gtcagtgagc atctggaacc caagtgaataatgttgagaa tttctaaaag 75001 tttaggaaac ataggtattt ccatcacttc cattataaaaacttcattgg gaatgttgga 75061 ttaataggaa gatcatgcac agaatgatta tttaaggtcctaaattgttc cacctgagca 75121 tgggtaagga gaacaggtgt taattatttt tgctgcgaggggaaataatt taaaaactag 75181 agggaggaga gggttaactt tgggaagaga gttcagagaacctgagaatg tgatgagttg 75241 tccccttttc cacccacact ggagaggggc atgccaggggcagttgggaa ggatcaggca 75301 ttcttcccat aaagtcgagc ctatctctct atgccagcagaacatgctgc agggaggcac 75361 aattcccacc attctgaccc accatttcct aggtagtcaaacttagggtg gcaagaacac 75421 ctaagattcc cacagtctag agaaggagga agagctggcaaggacaaaga ccagaggaaa 75481 tggcctcctt gtgcagggag agcccaccac ccactgggatcaggagaagg tcctgttatg 75541 aggagcaacg cttgtgaatg aagaagggtt ctcagctgggtgcctgaaag ccatcaaaaa 75601 tgttccatga tcacacaaaa gagtcttaca tattttcattactgcaaacc agccttgaaa 75661 atatatgtat gggccaagaa aactttgccc accgaagaagaacctccctg ctacaacaca 75721 cctgccagtg actgcccagt gataaatgct tgcaggccttgtgaaatgag gagatagctt 75781 tgcaaagggg accagagtaa aatacgtaaa aagaagggaagcccttatag agactttaaa 75841 ttggacagtg gctaagactt tcattcttac atgagattaaacaagtaata atagacagat 75901 tgagtttccc agagtatgtc agctgtacac aggcaggaagttaaaaaaaa tacatgtggt 75961 cataagaata catgtttgca cgtctgaatg gtaagtgttcaaaatccact ggatatgcat 76021 ataggatgaa actaaaatat agatatggga gatcatacaacctgtttgta tataagtaca 76081 agaggaaaac atggccaaaa atatttgttg ttgataaggaaggattttaa cactaatgaa 76141 ggaaaaggat aacgagagat ctgtgaaagg aaataaattatgtagatgca aatgacttag 76201 tgaatggctg taggagcaat tttctaaatt tgcaggtgatatttaattgg cagacattgc 76261 aaacagcaag gagcaattca atcagataaa gaacttgaatcattaagtac ttggcctaat 76321 agatggcaag cgcagataag tggtgaataa taagtcttgaatcaaaacta aagaacagag 76381 agtgaacatt tgccaaaggg aatgatacca tccaggaatggctcttagat ttaaggagaa 76441 cttatagaca aggacaaaca aaacttcagt aaaatattacaaataaaaag atttggtggg 76501 ttacagctta tccagctgaa gctgtgatga gaaaatctgacaacttaagg atagataaat 76561 cctgaaatcc acggttggct attgaaattc catttgttacctagaatttc atctatccct 76621 gatcatggtt tggagataaa attaatgagt aagataacatccaagcatat aagaggttac 76681 agatttgggt gaggatacta taaataactt ttaaagacatttcggatatg aaattctcta 76741 tgttctatgg gcataattgt gaggtattgt gtttagattgcttggtcatt tttatttaac 76801 atgccagttc tagtctacgt tagtctggaa attataaacatagagaaata tgctacaagt 76861 aaaagagaat aataaaaatt tattatagcc tagaaaggtagttgatgcaa tatataatat 76921 atttcctctt tctctKtttt tgcaatggtg aatactttactaaatgaaaa ggttaatttt 76981 aatattaaag agaaatggaa acatccttta gtacaacattaaaaccacaa atggaacctt 77041 taaatattca ctcactctag caatcaatgt tccaacctcatttaaaggat tttttttaac 77101 ttgacataca aatattcaga atgaatattt ggaagcagagccacgagcca taccttgaaa 77161 gataactgcg gacttactct tgcctgccat ttggRtacttcagatttcag ttgttctaag 77221 tctcaatgat cctgagtttt ctttttgtaa tttggtaattttgtaatggc aggctttaaa 77281 ataagtaaag gttttcttaa ctaagaacaa aaaaaaatccggagacctaa caaggctcag 77341 ctgtgtccag gtaggagatg actgcagttt ggcttcagaggctccctcta gcagtgtgag 77401 gacatctgag tgtctctcct actcatttta tatttcatttgatactgcac agaatcacta 77461 ggagaacagc cagaattgat ttacttctca tagagacaaactgtttctgg gagctagtta 77521 tcttcttatt aacaaagagc ttacattccc ctcccctccagagctcctaa aacatctata 77581 taatagctac attaagtgaa ttctacaatg attgaaaattttactataat gaagataaaa 77641 attcactcaa ttctagttct tttatccttg atttgcatttgaYtgaaatg tgtatttgca 77701 gtttgggcat ttcagtcagc gattgctcct gtattctttgcccataagca gccttcagat 77761 ggcctcttag aaaattgaag aagtctctac atgactggaaaactctttct gatggtctgt 77821 ttcttcaaag atatagcatt acctcttggg aattatagaaacaatatata ttgttaagcc 77881 tcttctaatg ctactgtaat ttataactgg taactttttcccccacttag cattcaataa 77941 gcatttattg gggcctaatg tgtaggtgga attttggagaatacataaag tatagaacac 78001 agagttcttg ttcttcaaga gctaagcagc tctatagagaagagagagac agaacgacag 78061 agacagagac agagagagag agagaagaga tcaacacatatccaccactt gctttactat 78121 ctatacttca acttttggtg gactcatccc atcttatggattcaattatc atctataagt 78181 agaccacttc cccaattgtt tctttaaccc agacctctcacctgaactac aggcttttat 78241 tcctcacaat gccttcagca ttttccattt ctaatgggtaccYcaaacat taagtgttca 78301 aaactgaatt tctgatttca cccctttccc Ycatccttgtctgttgtagt gtatggcaac 78361 tccaattttc aagttactca gtgcaaaacc tttgagttgtccttgaattc tcttctctct 78421 gtcacgcctt atatacaatg catcagcaaa tttgtctggctgtaaatctg aaatatacca 78481 taaatataaa tacgatcact tctcaccact cgaaagccactatttttctc tctttaggac 78541 tatttcaatg gccctttaac tgctcaccaa gctactgctctttttcctat ttctgttaaa 78601 aaatctattt ttaacagagc agccaaagtg attgttttaaaatgtcagat cgtattacac 78661 tctccctata accctccaat agttttcctt caactctaatttacataatt ccctataatg 78721 ccttatatgt tcaattatat acatatacac ttaccaccatctctgtgaaa tctctttctc 78781 ctacttccct agtatgagta ttgcttaggt ataaatcagaactcagaaaa ataagagtaa 78841 gaattaaaaa ggacatatgt gctgaaaaaa atagagcaatgaataaatac tggggaagta 78901 attagaagga agagattata cagaaaccat aaggcaaaaagttaatcaaa tttatcaagc 78961 atattatagg ggacatggcc aactccacaa tttgttcacctataatctca ggcaagctat 79021 ttaccttgtc tgagtcttag atttggcctc agcgaaaggaaagtggtgat acatcatgag 79081 cagggttgca gcacagctgg cataaatgga ggtttatcccacacatgtgg gctgaaaata 79141 ttagaatcca agctaccaag gtcagagtgc ctggctaagagcttcatatg gaaaaaaaac 79201 ctttaagtca atgacaaaat gggagagact tttttttttcaggcagtata tagccaagaa 79261 tctattcctt gaatgctgga attgaaggtt atataaaagcacttattact accaaattgc 79321 aaagaacaag cagagcaagt aagttgagag agagagagagaSatctttct tggagaacat 79381 ttttggaaaa tgactgtggg caaagcaatg tgaaaatatttagggagaca gaagactacg 79441 aagataaaat acctatatca agaagtttat attgaaattgagaatataaa acagatatta 79501 tgggataagt cccataggag agatacagaa aaacagagaaatgaaggata actgtaaact 79561 ctagggaggc aatgtcacaa actcaggtga aaatacattagaaaaggaaa actaggtatt 79621 tttcagtata tataatcagg ctggaagata aaagctggttcagagactag tgaaaacaaa 79681 tcaggctgga agataaaagc tggttcagag actagtgaaaactaaattgt tcctataagg 79741 actttaaaag gagatcagca tgtgagtaga cttggaggcctgctaaagag ggagctagga 79801 gaaggaatag cacgtggttt ctgagaaaca gtgtagcaataaggcttatc acacataaac 79861 taaactaatc atgatgtttt tgtcactatt gtgctgtcagagaaaaaaat ctattgaaat 79921 aatgaaacat actaattttg agaaacatca gtgtggcaaaacccaagaaa cacttttcaa 79981 atgaatatac tcaaattgca agatcaaaca tctattaattataataggta agaaaaagga 80041 aacattcaaa aaagtccttc aaagcgaaaa tatgtaaattagagtacctt cataatcaat 80101 aaacacttat tgagcatcta ttcatgtgct atcaatgtgttaaaaataca aaagtgacaa 80161 aaataattat tgctcaaaat tctgtgcagt atttagaaagattgggtagg gataaacatg 80221 tatttctcta agattacatt acattatgat aaggtttatggaaaaaatgt acatacctat 80281 gtatatgtaa aaatagaaaa gagtctaaaa gaactttttcaatctcaaaa cagtgtttac 80341 atatgaggaa atgtagYgat gactgttcat tgtttataaatatctgttct ctcctttctt 80401 agtaatacag ctcctgaatc ataatagcca gatacagacttcccacaata tgaatttccc 80461 agtttctctc cttagctagt gtggtcatgt gactaaattgtagccaatga tatttaagca 80521 gaaatatcat gtgatgcctc tgagaaatgt tcttaaaggaaggattttta ttcttattct 80581 tatcttcctc ctttcttctg gctggaatgt taataggatgacttgtgttc aagcagccct 80641 ctaaagacat gaRttggaag ctatgtactg aggagcacagagcaacaaga gaaaataagt 80701 aaaataagtt tggttcttag ctctggaagc gtgataaacctgggatagtc agccagggaa 80761 cttctttcat gttgattttt aagccagtga tattttgggcatttctttta cctgcaggaa 80821 gaaaataggc tgattttata taatttgacc ctttaaaaactagaatatat tcatacatag 80881 tttttacagt tcagtgttta aacttattta tttttcatcttagttttgct tccctatgtt 80941 ttgtaaatgg taaacttatt agccattctg tggtatataaaagtttcact tttatgtttt 81001 gtttttatat tggcccaact tccatgttca gctaaatacactaacataat ttaaattact 81061 gcatttcatt gggaatccaa gttagaaaat tccttttcccacatttttaa tacctaaaac 81121 ttgggaaatg aataacatta taagtacttg aaaataatattcactgattt atcatgtgta 81181 agccctgtcg aaatatttat tttctactga gcttggaagatattttaaaa tgattccatt 81241 aaagaaattt ttaaaatttt tgaaatatct atttacttaagatgttgaag aaaacataag 81301 catctacctt taccttccat tttttagatc tcatatgatatgacaaataa atagaaaatt 81361 agaggaaatt ttacaacagt actctaaatt aagtaagggtaccctccatg aaccaggaat 81421 tggcagattt caaaaaagca cactcctaag aaatttctgtttttcacgtg accctgacaa 81481 aatccattaa ttgtcctaat ttggaagggt acaaactgcagaaaagagca gggtattagc 81541 tcagcaggaa gcataatatt tcattgccaa gttgctgttgtgactcagta gcagcacttc 81601 tgttatcttc tgcatggctc ctcactcttc agcgagcaaattaggactca ttcacatggt 81661 gatccagggt tcctggcaca gcaagataga tggcaagccattgtgccagc cctccaatgc 81721 tctgtttgta tcacattgct tttctcccat tgatcaaagcaagttcctgg ctaatcccaa 81781 agttaaagga tagggaaata gactgcacca attgttggaagagctgaaca gtacattgca 81841 aaggggcagg aataccagaa taggaagaac tttgtggctatcttatgtaa tctgccgtgc 81901 tgatcagtta gactaataaa ccaagagaag aaatgaaggataataaattc aaatacctaa 81961 tagagatcag gctgttaagg tataaaagta aagttgggtatagtacctat gcacacaatg 82021 tgcttgaatt gtgagagcta aagtgaattg aagatctcatgaactttcca agggggcact 82081 gtctctcaaa ttcaacaagt tgcttgaaac agactccagcaattttcaca tgaaattgta 82141 agtttgaatt tctatgtaga ctctactaat tttgaattctggctgaagta ttttaaaact 82201 gtggttgaac ataaccacat ctgtaagtta cttatgaatcatggaccatc aacatgtgag 82261 ttttgataga gaagactgaa aggataacat tttacaactctattaaatat aatataaata 82321 gtacaattaa tatatatttt atataaattt gtgtcctatagacactgaag acccataggt 82381 taattacaaa aattgataat acatcatgtg cctccaactccaaaatttat atacaacata 82441 agtaatacct aacaaattac cagattaaaa cacaataaaattaagatgaa taacatttta 82501 aacaaagaca aaatctcaaa ccacttggaa gtaaaaatagcaaatgagca aaaactctca 82561 tacactgttc ctagagcaaa gaggaaataa aaactaaaagtatagactat ttagaaaata 82621 atgataacaa aagcactaca tgtaaattgt ttttaaaactttgcttacag aaacgtgttc 82681 atctttaaac tctacttaat tactaaatta aaataatgaatcaagccttc aactcaataa 82741 acaaaatatg aagccattaa agcttgagtt aaaatgcattaaaatctgta ttttagaatt 82801 cataaatcaa tagggttaat tgaagggaaa aaacagttctttgaaaagat ataaaacaaa 82861 aacaagcctt gggcatgtct ggttttggaa aatgaaatgcataaataaaa gtatcaaaaa 82921 catgtgtaca ccacaattaa gaaaaatttt cacaagcatgatggaacagt aagtacaact 82981 ttatcgtaat tatttgaaag aaacgtatgg catgaattattatcaaggaa aacatcccat 83041 cattaaaatt gacccaagaa gaaatagaag acaattaaatatatgtccaa tatgacaaaa 83101 aatgttattt gtttacaaac aaattaataa tgttatctcctgcctgtttt cttaatttca 83161 ggaaagagta ggccaaatga ttaagacatt tcataaaagttaYccaatta attctgaaga 83221 gctagagtaa ttcccaaaag aatggctgaa aatgaaatcacatacaaaaa aagaaaaaac 83281 attcaatatc actttagtag tggtaataaa aatacttattaagtgataat tgaattcttt 83341 tacaaaattc ttttggatat gaatatccaa aaatcttaaatgtcctttca tatttaatcc 83401 gtcagcatat tagaacatag taaagtatta ccaaacagggtttattacag aatgagcttc 83461 attcaaccaa aagataacag gagaatcttt ggcaaaatatctaatagtaa gcaaagttgg 83521 agacatggat tagataataa tatgaaatca tcatgtgttctaaaaatgaa agaatggtat 83581 taaaaaatca aaggaaaagg ggaaagtagg ggaaaataggatatcgtaat gcactttagg 83641 gaataagtgg aagtaacaga ttttatttaa aattttcaaaacgttaactg gaaaaaaagg 83701 agtaaaagca taataaaatt tttaaatatt gaggttaaaaactataataa aagtgcaaat 83761 ctttctagat agtaaaagaa aaacctacca aagaaaacatgctacataga aaaacacagt 83821 aaatataaca aaatctacac aataataaat aacataaagaatagattaaa taagatacat 83881 ttttgggata tggatcaaac actacaccct cataatatgccattttctca agcatacatg 83941 gaacattgac aaaagtcaat catatattga ttggcctcaaagaaaacact attaagttcc 84001 atttaagtac tttattacaa atagtgttcc ctcatcaaaatacaataaaa ctagaaattt 84061 agaagaacag taagagaagg cttttcacct gaaattaagaaatcatcatg ttaaacaact 84121 gtagaaaaga gaaaaagaaa caaaaattat ctttaaaattagtggtaata aaaatactat 84181 atatcagaat ctgtaaagga tacttaaagg cgtaattagtagtcttggcc ctccccttcc 84241 cttcccttcc cttcccttcc cttcccttcc cttcccttcccttcccttcc cttcccttcc 84301 cttccccttc ccttcccctt cccttcccct tcccttcccttccccttccc ttcccttccc 84361 ttccccttcc cttccccttc cccttccctt tcccttccttcttttcttct ttccttcctt 84421 cttctccatc tccctcctat ttttcctcct tcccttttttttccaccctt tcttccttcc 84481 tttagcattt cttcaatcct tactttctcc caagcaaacctggaatcttt acattgtaat 84541 attatttaaa agccaaataa aatcaaataa tatttaagaaaactatcaag aaatttcaaa 84601 tatttacaaa aatttattct cttgaacagc aagagaaggaaacagtttag taagttagat 84661 aggcagaagt agacctagta cttgtgaaat gtgtgccaaaactcttcagt aactattttc 84721 cttctgcttt ctgtagaaaa tatttaaagt tgaaatgaaagcatttcctg aagtgatacg 84781 tgaattacta caagcaatat gtacaaaaca tgaaaaacagaggtgatatt tagtttggaa 84841 ttgtcatatt caaaatcaga aataaaattt tcattctgatagagcagtga gtttgatttt 84901 attctcagat taaattacag aatggattat taatcagatgtgttttagat gattaaaata 84961 gcagttcatg tttttgatag cattagctat tcaggatcaaatgaatatct gattaaatat 85021 gtattttgag ttcaataatg ccttttacaa agcactUaaaccctataga caaacgtggc 85081 ttgatggcag tgtagtaagg aatatttata ggtgattgaaaagctatatc taaagtttta 85141 actagtgagc tatcatcaaa gtgcatagaa ctttagaggcatattgccat actttgaact 85201 tgccaatgta tgtttcatca atactttact agtaatgtgaatgaagaatt aaaggcttgc 85261 tcctcaactt ctgaatggct caaacatggt aaaataaagttgacataatg aatgagaagt 85321 atagattaca gtagatgtga aaaatacaaa agaaccaaagaatgaaatta aagagacaaa 85381 tataaaatct aacatttgct tcaaYtgcat aagactttgtgggctactag tttgatagtg 85441 tttattttaa aaatattttt aaaattttat ttgaccttaaatttagcata aggcagtatt 85501 aaaaatcacc taataaaaat gatacagcct ccaattttatttaaaaagtg tataatttat 85561 atcaattgag gaaactgatt atttagactg tgttcacaggagaatgacaa gtattttgag 85621 ggacagaaaa tcatatcttt ggaaaaagtt tgaaagcttgtcatagggca gatctattta 85681 gattgataac ataatgtatg gatgacaaaa ctaaaacatctaataaagag tacagggaag 85741 caggtattga ttcattataa aatcaaagtt ttataataattagaatattt caaatgcaaa 85801 attttcatct tagaaaaagc aactggactt ggtgtaatgttaaaataatg catgtaaaac 85861 acatgatact tggtatatag taagcactaa ctaaatgcatactattaatc ttaccaatat 85921 aatgttctta gcattatatt caagaataaa ttagatctttagttgttaca tataatcatt 85981 tattttagat taaatttaga gtaaatgtat ttcaatttgtctaagcttct aagattcagt 86041 gattatattg cttattagtc atgctccatt gtatcatccatccatatcct tttcatgact 86101 tgagagaatg caaatcttgt atcttctctc aaggaaaatattatgactat ataattaaaa 86161 taattttaaa aatgtattta tattccagtt ttagtgctagtcctaatcaa aatttaaata 86221 atcattttac aataaatatt tgacatataa tattttgtaatatattgaat tttctatagt 86281 agatgcctaa ttatcttagc tatttgcaaa taatgtaagtgtaaatatac tgataaatca 86341 agaaccttac tttaattatt gacataatta ttcaggatattaatctttta tcattgctat 86401 aaaacagtca attttcatat tgccattttc tttttattatKtaaatacct ctctctgttg 86461 ggaatgcaga caaaaagatg gaagagattt ataataattaggtaatataa gtttagtagg 86521 ggctccatct ttatttattc atatattcat agcaagtatttattgaccat gaattctatg 86581 ccagaaaatg tgatatgtaa tttttttaaa agtcacaatccttgctttca tggagcttat 86641 ggcctagtgg aggagtcaaa cattattgaa aagatcatataaacatatgt aagattgcag 86701 ctgtcatagt ggctaggaaa gtgaggaatg taaacttctgatagtgtaaa caaattattg 86761 ttccaatcaa agagatagtg gatagcttcc ctgtgaaaatgaaaatcaag ctgacatata 86821 gatataaatg agtgtttact tgaagtttca ggagggtcaatccagactgt gggaacagca 86881 tgtacaaaga ccatgcatca ggaagaactg ctggaagggaaaatggtaca gtatgaatat 86341 gttgaagcag gttggggata gcctacRtaa aggtgtgtagaccatgtttt taatctttct 87001 cctaagagcc atgcaaattc attgaaaatt tttaaagtgtggcaggagac aggatcagat 87061 ttgctctata gaaagactac tttggctaca atagggagactgaagaggaa gaaaggtaga 87121 Yttcaggtag tcttacaagg agctggagcc taggaaaacaatgaaagctg gaaatagtaa 87181 agaaatgaat ggatattagg atgctcagga ggtaaaatcaataatgatag atttgatagg 87241 gggcataggc tggagaagat aatgaagcta acaagttttatgacttatat aaaaagacac 87301 cattaaagca agagatgttg gaagaggact ggttgaatatcataagtatg atgttggcca 87361 tgttaaatgt taaatttgag gtatttttaa gtcatttagtagaaatgtca attatgtagt 87421 tgaataaatc agtctagatc ccagagctca gatacacatctagaaataat acatttgtaa 87481 attgtgtgta gatggggaca atagcagtaa tgaacacagatggaattgcc taggcagaag 87541 agctcaaatg agaaaaggat gtaggcccaa gtccagagaactgctaacat gagcttgaaa 87601 aaggaactga gaaagaggtg tatgttatgt catgaaattcaagggaaagt tgtgtttgtg 87661 atggagggag aaatcatcaa tgctaaatgt caagagacctgattagatga gctttgaagt 87721 atatccacta cgttcaacaa tacaataatt aagaagtactttttccatga ggaatagggt 87781 atccaacaat atggggaaaa agagttttct cagagtgactgggatatata aaaatggaga 87841 gaaaatgtgg taatgtggag taaccagaga gtagagggaagggctcaatc tctttgattt 87901 cattttaaca agaaacaaag aatcttttaa tgggaaatctagttaatagg atgatgttga 87961 atgcatgaga caggaactta agtatctgaa atagagagagattgatacat ttaatgatgg 88021 aaggttagcc ttaggaaagg ggacagctcc tgtattgtggaagcagataa atagagtaga 88081 ctgggtatgg gctataggtt ggaacacaga aataagtatgatttttaatc tcccatcttc 88141 tatgttctct atataataac agtaggatta aagatgtgacaagtcgttgt aaaaagtgga 88201 caagtgagtt gaccaaataa ataaaaacct aagaagggtaagaataaata attgtctttg 88261 attatttctg gttatgttgt ttattgatat ttaggaagttttagaactgt ctttctgaat 88321 tagtggtcct tggccttttt ttttttatta tactttaagttttagggtac atgtgcacaa 88381 tgtgcaggtt agttacatat gtatacatgt gccatgttggtgtgctgcac ccagtaactc 88441 gtcatttaac attagatata tctccaaatg ctatccctccccctcccccc accccacaac 88501 aggccccggt gtgtgatgtt cgccttcctg tgtccatgtgttctcattgt tcaattccca 88561 cctatgagtg agaacatgcg gtgtttggtt ttctgtccttgcttggcctt tttttaaatt 88621 ttgcttcagt gcagcacctc taatataagt aaggactaccacatactcat tatccattac 88681 agtgttctgc tgtatcattg attctgaagg atggagtgaagcacctcttt aggaagacta 88741 tacaaaactc agagatggga aagagagaga gagagagagagagagagaga gagagagaga 88801 gagagtgtgt gtatttgtta gaaaaagttc attatatgattctgatatgc ctcccacagg 88861 gaggctagaa atttctcctt gaaaatagaa tagccaaatgttgaaaatag accagaattt 88921 aggaatttgt ttatagatat atgacaagta attgttaaatttaataaata gtgtagcaca 88981 tacaaataat cagctccctt ttcctaaata aaaaactttagctattttta ttctatttta 89041 aaaattcact tcgtgatatt taaaggtatc cattattaaaatcagtaacc attccaattt 89101 ataatcattt aaaagtaaaa catataatta tttattagaaaaatatttgt gaacatttta 89161 taaatattat caacatgttg tcagtaaatt cctcctcaggtggaattttc tgactttggt 89221 tgagtcatta aatgttttgg gtcactagtg atcaattagtaaattactga tccatagtac 89281 tttaatggcc tgaaaaagta aatgcatctt ctgagcacctaattgaatct tagttattta 89341 ttcatcatta aaatcaagat aaatgagtca gctcacaataatgaaactga acagttacat 89401 tcatttcaca aatagattaa actaaagctc agaaaagacgggacaaggga gttaaacatc 89461 caggttagat tttccatctg aaaagaccca attcccaatatcttgctata aaaattaaat 89521 acatatatga gtttgaaagc aatatttttc tgcaagttcattttcatata gcgttattaa 89581 gtttaatacc attcagttaa taattgtaga tccatgRtacgtatactaat atggtcttgt 89641 tataatgtaa atagtacagt ttaaacagca aaatttcacactgttttaat ataaaaaatt 89701 tcctagaacc aattaccctg agtgcctact atatggttcatttggaaaat gaatgatagc 89761 tgcaatatgg taaaatgatt atgagcacag ccttcggaataagtaaaaca tagctttaat 89821 atctaacttc tgtacttact aactgagatc tgaacaagttacttaaactt ctaaggttta 89881 gtttctccac ttacgaaatg gagaggaaaa cacataattcatagttcatt gtaaggacta 89941 agtaaaaaaa aatacttaaa tacttagcac agggcctagcaacaaagata tatgaactcg 90001 gtacacacag acatacaaac acgtacccac actgcttaactttagctaga atatttttaa 90061 aagcattatt agtttgagac tagattcatc caagttggtttagcattcta ctagggatgt 90121 tacttttcat atctttcttt gtttttgttc ccaaaagctataaacacaag ttttatcttc 90181 tttcctgaaa caattaaaaa gaaaatacaa gaaaatgttcaaaaaataat ttactagtgt 90241 ttttgtttgt ctggaagggt catcctgcag ctttaatcacagctgaagat cattatttca 90301 aaaaagacac atcttttaga tatttaaata tgtattatcatattatgcag tttaaatgtg 90361 aagtcatcta ccctctggtt tactgagatt ctgttacattttaaataaat agtaaatgct 90421 gtagttaaga gtgctatcac tgagaatcta agaagacacattagaaaggg attattactc 90481 caagaagaat tctctttcct taatcattac tttttcattaaaatatttct gaagtttata 90541 caatacaatc atagtagtga ccttaggcat atgacaccttagtgctataa aaactctaac 90601 aaatctgttt agagcccaat aatataaatt tctgtgaagagaaatataaa ccaatggtaa 90661 aatgccatgt tgaagtttct aaatagccca cctagtaatttacatgttaa gaacatattg 90721 aatatcttgg gaacatgttc ctagaaaaga ttctccattataagcttttc ctttactgtt 90781 aatgattgct ttgataaaat gacccacgaa aaaagacaattttctccctg tgttatctgt 90841 gtctgaaata ttcttcactg attttacata tgctgataatatgaacaagg taatttgtct 90901 gactctggtg gggcaagatt ttcagtaaga aatgcaaaattcacacatag ctcttctgta 90961 agaagtctWt gcagtgtttc agtttatata aaaagatttatattgcctga ggcatctatt 91021 tttttttcct cgaggcaagc tgaagtggca caaatgaaattgtttttcac actgttaatg 91081 tattcctttc tctttcaatt tctcagagag ccgacatagggatttctgct ttaaccatca 91141 ctccagatcg tgaaaatgtg gtggacttta cgacacgttacatggactac tcagtggggg 91201 tactacttcg aagggctgaa aagacagtgg atatgtttgcctgtcttgca ccatttgatc 91261 tctctctatg ggcttgcatt gctggcacag tccttctggtgggtctactg gtctacctct 91321 tgaactggct taatccccca cgattacaaa tgggatcaatgacgtctact actctctaca 91381 actccatgtg gtttgtgtat ggatcttttg tacaacaaggtaaggagcaa aagtacattc 91441 tagtatttaa aaaaaataga atgtgatgtt tccaaagccatgtattcagt taataaggtt 91501 ctgtatctga catcaataag tgttctaaag ggtactcacagaaatgctca gaaaagaaca 91561 tttgctactt cctcctagtg gatctcagac acaccaagtttccagcaata atggttcatt 91621 gtcttttgat gtaagtttaa tttcatcaac aaaggcaaatgtgaatgtca aaggtaaagc 91681 tcacagaaaa catcaagatc agattttcct ctccaaactctgactttctg agaaatagcc 91741 ttataaggat gaaattactt attaccagag aaggtactatttaattattt acatatgtaa 91801 gaaatgttag gacttgttct ttgttcagag acattatttcatgcattaaa aatattgtca 91861 ttgaaaagac tttggttaat ttgagactga tgagaaattttgtgttttat cttttacttt 91921 gatatgtaat ctttactggt gatttaactt acaatttcactattttctaa agaatgtcat 91981 tgctctgtta tttaatatcc aatctgccca actctttaaacttgtccaat taattatttg 92041 gctctcactg tttcaaaata gaataatatt tattgcattccttacttgag actattgcct 92101 catagagaat gaaatagtaa gaaaattgat agtctcttgcaagctctaca catatattga 92161 agaggaggat gtttgctaca atggagacat tatacttttgtgctaatatt tacaactctc 92221 aataacatct ttctttttat tcctattttc tcttggaccttccatttaac ttcatttttc 92281 tattctaatt taatcaaaga ttagtctaga accttcatctgacttgctaa cttctaatct 92341 agcccttaaa ccttttgtaa gcatgctttt ccctccacccttccatatcc agggctctaa 92401 gcttacctgt tactgtatat ttttcacaga cacttttagttctttcatta ataattaatt 92461 cagtaaatat ttaatccaca ctatctaagt agaactttcctaagcaatta gtgtatgatg 92521 gtgagcaaga aggtctttgc catcattgcg ccacaacagaagtctaatga gacacaaaca 92581 tgtaaacaca ggattacaat tgtgggtaga aagttctgtggtaggataag catagactgt 92641 taggagatca taggtgtaga agcacttaat atatgttaatgtcgcagaga aggcttttta 92701 aagtaacact ttatacagag agacagagtt aactatggaaagtgcttctt gaggaagatg 92761 aatggagaaa aggagacata aacggttagg atggaatttcagaccaagaa gtgagagaga 92821 aattaagata gtccaagcag agaggatcta gtaaagatgaggctgaagct acatttgtta 92881 ttataaggag tttgaactga tccttgaaat gatcagatatttaaagataa taaatgggaa 92941 aatacagatt caaattttac tctagaaata ttctggttataatgtggaga atagattgaa 93001 aaaaggcaat gtgaaaagtc agacaaatta gaagactattacaataactg agcaaagagt 93061 tgatgtggct tgctcaaagg taaggtagct ttgggagtcaagaggagagt attgatgaaa 93121 tggaatcaac aggctttgat tacctatagc gtggtgtgggggtcagtact gaagaaaaaa 93181 ggagataaag ttttatccta ggtttttgaa tagaaaattatgtatattgg atgtggatct 93241 catttactga aaaaataaaa cagaaaaaac agatatgacaggagagatga tacattcatt 93301 ctgctgcaca ttgagtttga gctatccgtg aaacatccaaaagagtatta ctaatgtgtg 93361 cttagtggtt tgatatattt gtcagatgca cagaagagacatctggactt aatatatatt 93421 taggagatat tggaatgtag gaaattttga agacatgggagttaatgaga tcacacagag 93481 tagttgctct taattgcaaa ctcctgattg aggaccactggcccatacaa agcctaggac 93541 agagctctaa ggaaaggaat ggatagagga gcactcagtggtgcctgata aggagtgact 93601 ataaatgcac aagtgaaaat cagagcagct agtagtatacgtttggttga ctgtgttaaa 93661 cctttcaaag aatcaaatga aattaaggct tgaaaggtttttgttggatt taacaataaa 93721 tttattattg aagcaaaaaa ggcttgtctt tgccctatgatgtctgaggt ttcagctaga 93781 caattcagag gccagaggct aaaatcatcc aaaggcttgttcatttcact cacatgtctg 93841 gtggttgatg cggtctgtgg gctagtggcc tcagctttttctccatttgg gcctctccat 93901 gaggtcactt tgcatgggat agtttgactt tctcacagcatggtagttgc tttctccaca 93961 gagaacatcc caattaaaga attttatgtt ccaggttgggagtcataaac ttgtgtgatg 94021 gtgtgaattc ctcttgcaga gaacatcctg gatgtacccacctcatgggt ttttatgaga 94081 atttattaat gaatggttaa aattgtgaag ttcttagatataacttatat aagtgtcaca 94141 taagtgttta ctaaataaat aaaacaatga attagactcagcggcagata attgacatat 94201 gtaaaaaaag ggataacaag acaaatgatt gtagatcattttgatgtaSa tattgattga 94261 aatagtagat catggggtat atggagaact agaaagagaaggaccaagaa tgcgaatgta 94321 aagaagagta agggtagaaa ttgatagagt aggcctggcatggtggcaca tgccagcact 94381 ttgggaggcc gaggcaggcg gatcacctga ggtcaggagttcgagaccag cccggccaac 94441 atggtgaaac accatctctg ctaaaaatac aaaaaaatgccaggcatggt ggtgggcgcc 94501 tgtaatccca gctactcagg aggctgaggc tggagaattgcttgaaccca ggagcgggag 94561 gttgcagtga gccgagattg tgccattgca ctccagccgggatgacaaga gtgaaactcc 94621 atctcaaaaa aaaaaaaaaa aaaaaaaaaa aagatagagtagttaggaga gagtagtaga 94681 ggtctcaata agattacaga aaaagagtga aaacataattataaatgagt ttggagtctg 94741 tatttataaa ataggacatc taagcctaat tttttttgaaatgtaataga ggagaacatg 94801 ttgtaggtat gtggatacaa attccagatg ttaagatttggtgaaggact tcaaagctga 94861 tcaaggaact gagagataaa gggttaggtg agccagctgtaatgatggct tggattagac 94921 tggagaaaaa gaaattgagc catgtgtcaa agttgtgaataaataaggaa ggatgacaag 94981 gatgttgata gatatgagca ttgaccagaa ataggaggtgacatacctag atataattaa 95041 cctctgtttt catcttactt cttccaattt attcagtttttcagtgtatg atttcaacaa 95101 ctgccaatat gctgatgact cccagatcta catcatctattcttgaactt cagatttgta 95161 tattcaactg ttacctaggc aactctctga ctataccagaggcaccataa atttgaacat 95221 gccaaaaatg taattcattt tagaatttga accttcttagcctctcacat tctctattcc 95281 agctgatcca ccaccaacca ttcagactca tgttagaaactctgaaaata gttttcattt 95341 cttcctcttt ctcatcttgt atatccaacc ataatcaagtcctgtcaatt ttacctacta 95401 aatatttcta gaagtggaaa tctcttctta tctagaaagattagcctaga ttgtcaccta 95461 gattgaggct acctcatttt tcacctgaac tataggagtctcataagtct ttcttcttcc 95521 agtgttgtct cctgcaaata tacgattcac atacttgcatatatacccaa gttatccatg 95581 ccatggaaag ataaacacat aattcccatt cttcagttattttcttggct tcccaatgcc 95641 cacagtttag aagtctgagt tcttcagagc ataaacaatattccatcacc tggctcctgt 95701 ccaacactca gttctcatct cttaccattc gtgcctcacatttaattgcc cagttactcc 95761 aaacttacgg tttgaccata agacaagact ctttcctaactcttcacctt Kgtatctgct 95821 ctttccaatt ccttgagtgc ctttgttacc aacctgattagactctactc ccactctctg 95881 tctttataca gcaagttcct attcatcctc taattttttgcttttatttc tctactttca 95941 agaagtcttt cctgggattg agaaccatct tctatactcctcaacatgta tttgtcatag 96001 tacttaccaa ccatagtgta attccttttt ttaaacatgtctccacctga gtcctttgaa 96061 tataagatag tgtactattt aatctgtgta gtctctttgcttatagttaa gtgccttgta 96121 catgttaggc atttaataaa ttcatattga taaatttaatgaatggatgc taaatactta 96181 actaaataaa gcatggaggt tagctgcaat attttgtttattagattata gtctcaaata 96241 aaacactaaa attatccaca aatgacttaa aactcactaaaccaaaaaga aatttatctg 96301 taatgtcccc actgagttgt tgtaagaaac taagaggagtttataagtaa ggcatcctct 96361 tggttgaagc ctcatttgtg aagacgttga catacttatgaattggcttt ctgaattctt 96421 cctccaagtg accaaatttc aataagtttt ggagcacacccaggaaaaaa gataaataga 96481 attacccata tggttagcat gcttttctag ttctataaacatgcaacaga taacaaaagt 96541 tataaatatg ttaaatacct ttctatccca ctgctcccttaaatgagttg cacagcctgc 96601 aggttgattg tacatttata caaatttata atgacagataatgctgaatc gcatatgatt 96661 agctacttca tctttgaaaa gctgctattM ttccacttgctaacaatgaa aaggtgaatt 96721 cattaaaaga Yactgagagc atctttatgc tctgattaaaaataaatgaa gaaaaggctt 96781 ataaacccta tgaaaaagag aataaatcaa tgacagtgtccatgtatagg caattgactt 96841 ggaactggtg atatttccaa taaaaataca gtcttttaagaggaagtaca gctgttattt 96901 cagaattgca gtcttcattg agccactata ccagattgaatagggataaa gaggcatttc 96961 atgtgttatc agacagattt ctaaaaaaag agttcacggacagccaaaag cagaaaaatg 97021 ttaaatatat gtgagttgtt tactaatcct gacattttaaaaaatctgta acttcaaagc 97081 attctggaaa aatagctcag ggaatccaac tgtgcaaaagcagtatggtc cattatagag 97141 ataaactcag tacatctgtt tggctggaac attaagagcttgtgaattag gttgtaaaat 97201 gcacgaatga gagaccaatt ttatacaact tttgttgtcattttatgtcc tccgttatct 97261 ccatgtgtac atacatctcc tttcaattaa tattcttaagtgaaactgga tttgattttt 97321 agaagtctct tatgtttagt ttattggcag tgactctatcatctggtctc cagaggactt 97381 caatgacttt gcaaatgtag ttgtatgtaa tttKctttctgtttacatgc aaagggaaat 97441 ttcagaatta tcttcagatg tttgtgtctg attatgggaaatagaaatat ttatagtcat 97501 gtaaaatatt tgaagcaacc acatatatgt ttatttagcagcaagcattt attaagcacc 97561 aacaatgtgc caggaacatg agtagcaaaa ggaaaataaggaataactct taactagggt 97621 ttaataaata tttcacgagt atactatgtg

Following is a genomic nucleotide sequence of a PDE4D region (SEQ D NO:4). >5:58309301-58408050 1 ctctaaatat tttccattga tgaatttcta attgtttttggcctaagtag tctgaaaaca 61 atacacagat ttagggttgc aactaaaaaa acgggaggatttctaagtga atgcttttga 121 atcctttgtt ctcattatac atcagaatgt ctgtgtacagcagttaaggc ataaaggaaa 181 gtaaaaatgc agttgaatct caatactgta ttttgcttttcagaactgca attcttcaat 241 ttacattaSg catttaagat tgttcaggaa gcaaactaaaataataaaac aaaatgtcct 301 taaatgtaat ctcctttact tctacatcac gccagtgtgctactgggtat attatatatc 361 ctcccaatat atatttaagg aggtggtatg ggtccaatagtaagaaaatt ttcttatttt 421 ttcctgacaa tattctgcta aaaatcattt gaatatgctgcactgtgatg cattacaagt 481 ttggtagata tcagtattca aagttattta aacaaatactccattgatgg tataaaaata 541 aaWccttcaa aatgcataag accaaatata tctatatttgctatttttac atgtttttaa 601 aggtacagta ttaaacataa agctgtccag gaaaaattttcctcaaaaag aaacgtacta 661 aaattctata tgtagattct tggcaatatt gttcataatcatagtacaaa tggttcataa 721 tcacagtaca aatgtgaagc ccttcaaaaa taacatcagttgtgagctgg ctaaaagcta 781 ggagctgaat tctggcggat aatgagggct tcttatatttactctctata cttttctgca 841 tttctgaaga tttttagcca agaaatggct aacaaaattcaaataatgga agtctatata 901 ttttaaaaac atttttaaaa atctgaatca tccacagatttaaagggagg taaggaatca 961 tgactacttg gaYaaccaag actgaaataa gttttctaatttttttagaa ttcaagtaaa 1021 tctctaaaac aaaggggaac ttgtccttca aaaagaaagaaacaaagcaa acgacRaaca 1081 ctccaatctg aaaaatcctc agagtaacag aaaatgaatcttttaaaatg tgttgtgatt 1141 ggctcccagg ttcaagcgag tggctcaaat accaaaaatctgtgaagtga tgtttcattt 1201 aggactgaat aaggtttttc tacctccttc tattgtggagacaggatcac aatgctaatg 1261 ttaccacagg gcatgSaaaa agcctgccaa gcatggacacaatcttattg aattgtttat 1321 taattcattt cttgagattc acaaaacagt gtaataaaaactttgttcag ttttgcaagt 1381 ataatagaca atcaagtttt cactttagta tcacttacatagttaagtaa tgtcaatttt 1441 taatgtcacc tagggcttta tagctaaagg tgaaaaaaacgtattcctcg tcaggttaaa 1501 gtagtataga tttttagaaa cagccacaaa ttaattttataaactttatg tgcaacatac 1561 taactggaat ccataaatca gtataagtaa tttggcccRataatttattt gcaatgaatg 1621 tatttaatta ccatatattt ttcaagcaca attcaaacatataccacatg acaaacagaa 1681 gaactactgc gtatctaaaa aagctatttt aacactcaaactacaactga tgaaagttat 1741 acaatcttat cttgaccact attaacacgt atttcttcctagttcatgtg taaggaaaga 1801 atacataact gatagtttga acaaattaca gtagagtctaagctttctaa ataattttgt 1861 aatcagagta aggagttttc aaaagctaag taagtcttacctttatgtag ccccaagaca 1921 ctgacagtaa atagtttgct tcaggcattt ttgattgattatatgtatat atatatatat 1981 gtatatatat agtaagctct aaaatgtatt tagcttcaaaatctcgacac tttaacaacc 2041 tccacaagcc acgcagagta tgagttttta aagatttttggtaattaagt atacataaga 2101 aagcctcgca ttaaatccca tccagccgtc ttcccatcgaatacatgcca tttgtaagac 2161 tccagaatgg cacaaaattc agcaactatt ctgaataaagaaaatccacg tgataaagga 2221 atatctaaag atctaagggt ctgcatcttt ctctcccgagctccagggct taatgaataa 2281 tgtatgtcaa gatgcttagt tgcaagaaag gctagtcctgtcaaaagcta ccaaataagg 2341 aactgcaggg aagccgggaa gagtgacaag aagcccctcccataaaaccc caactctgca 2401 ccaattgcat tccagctctt agcctataag gcgagggggtgtggcctcgg tgcaggctgc 2461 tttgggattg gttggcaagg gccagcctct ggggaggctttctcctcacc cgacaaacgc 2521 cattgaataa tggtatccct gcagctctga ggccaccctgagaaaagatg aacggggctt 2581 ccctcttgca gtgatgaata atgaccataa aactcctcaaaattcgtagt tcattcttaa 2641 gaattgccca tccttttgga gggcattttc tatgaattttaatgtcctct ggcttccctt 2701 ccttccaaat aaaaactgct ccaaaaatgg caatcgagcattttaattgc ttccYtctct 2761 caaatccaaa acaagtggct ccctggtgat gtagaaatgactgtagttct cccctcatcc 2821 aacataaaag catagggatt aaacaatacg actaatgtccagaattcaca gatggcacag 2881 atggcaatga cacattccct cacctgtgtg Scatggctgctctcagctcc ttttgtttct 2941 attgttatgt atgacagagt tctaaaacct tcaaaatctaggggtgggac cacttgcaat 3001 tctgggggga gggaaatgtg aaagctaggg cactgtgtatgcataaggaa tgcagtgttt 3061 tgtatataga actcaggatc tgtattttga tcagaagagaatattttatt tatttattta 3121 gagacagagt ctcgctgtcg cccaggctag agtgcagtagtgcgatcttg gctcactgtc 3181 acctctgcct cccgggttca agcgattctc ctgcctcagcctcccgagaa gctgggatta 3241 caggcgccca ccaccacgcc tggctaattt ttgtatttttagcagagacg ggatttcacc 3301 atgttggcca ggctggtctc aaactcctga cctcaggggatctgcccacc tcagcctccc 3361 aaagtgctgg gattacaggt gtgagccaac gtgcccagccagaacagaac attttaataa 3421 ctgtacacaa agttaagaaa ggaatgagtg gctcttaggaatccagaatt gagctcaaga 3481 gtaatcagac attttacgag gagggttaaa tgagaaatcaaagttctttc tagaaaaaca 3541 gcattttcta tttggtttgg tgtagtatta ccctgtaactttaatactaa agaggtcttg 3601 tgggtgagaa acaaggaaaa actaggtttt agttgattttagcatataat tctgtgcatt 3661 ttacctcctc tccatctcaa agcctgtgct tactactctgtgctttaact tgctggtatg 3721 gctactctgc agtcttcctg acatttaact atttccacatgtggcatgtc tgtcttagag 3781 agcatgctct ctgataagca ctcctgtaac actgtgtctctctgagtttc tattggcctt 3841 tagctgctac agtcctctgc ttttgttaac atctgttgtattcatttcca tcactcttac 3901 tcctctacaa aaggaagaaa ttaaagctcc aacattccactaaagctacc cccagaggaa 3961 tactaaggtc ctccttttct ctggtctccc tacttctctgaagtgttcca catgtttgat 4021 cattcctact actctccctc aactcccagg atcctgaacttctttaattt tccctaggcc 4081 cacaatcttc aatgaccact tctaacttga acttcagtgccatatttctg aatacaagat 4141 tctcctctcg aatgccctgc tagtcacgac aaactttttcaaaactgtga gacatttttc 4201 tatttaaagt agttcttagt ataattactt ccatttcagtcagtgagcct atatttcttc 4261 cagtatctca gatggaatct atgactcttt cctaccattcaacaggtgtt tcatgtgcgt 4321 cagacactgt caggtgttta atataaacca tctagctgaatccttgagta acccggcatg 4381 ttcccctata cggcatcacc ttccctcctt cattcaactttgttagcaag ccctatcttt 4441 tatccagagc tgtttctttc agtttRttcc ttaccattacccctcccgta atctgaatcc 4501 aaatatttaa aagactgaga gaaatagaaa gtaactttaatatcaccccc tctaactccc 4561 tcatttccac ataaagttga gacctagaga gaaaaaatgattttcccagg ttcactctgg 4621 tactgagaac aagaattgca agtttaaatc ttttgtgcacaatcactgac aaataatctt 4681 tttagtgtgt cagcttcctc atctgtaaag tggattactgtggcagctag tctccaaaga 4741 tactcttaat gagccacact gtcctacact gaatctgagttggcttgtgt aaccaacaag 4801 tgatggtgca tgactgctga ggctaagtca taagaaatcttgcagctctc atcctgcttt 4861 ctttgaatgt gctattggga cccctaagcc attaggttgaaaaaaaaaaa ggtctgacta 4921 ctctgagagg ctatgctaca agaagtccat gttaacttcacagacagtga agagagaaag 4981 cgcaagagag cacttgcaca agaaagagca cacatgcctggccagtcttc agcaatgcca 5041 gccaccccag cccagagcca gacctgaggg gcaagaagccatcctagcca tccagccagt 5101 tcagctttca gatagctacc cactgggctg caaccatatgaggaatgcca agcaagaatg 5161 tctagccaag accagttaac ccacaaagcc atgaaaactaataaaaatac attcatattt 5221 caaagtatta ggtttgaagt gatttcttac atagcaataaataaccagaa cagatacata 5281 tgaggattaa atgagaccat atatgcaaag cacttaaaacaacaaatggt acaaagttga 5341 agataagtag gtatcagaca gtaaagatga cagtgacaatgatcattatg ataatcattc 5401 tattatacta atatttaaaa aaattctctg gtttgtcatttgtttttaaa atatatatgt 5461 tatatttcct ttcaattgca tcacagcact ccatgtctcagggccaaaat gctgtaattg 5521 cttcttagct catcttcttg cccctggctt ttccccttctagtctcatat tgacctctac 5581 tgccagatca attttcctca aatccagccg cagcctgttacttcaaagct agtaatgcct 5641 gcttcatcac atccctctgt aaattccaga ctgccatatctgaccttaat ccccaacgcc 5701 aagcctattt tcagctgctc tattacattt gctctgttcctgaaagacta gtaYtcctcc 5761 aYcttcacca tacagctgac aatcccatct ctgtcttaccaaattctcgg cctaaaatac 5821 cctccttctt ctactctgcc aacctcaaac tggttttctcagtcagattt aaatcattca 5881 aagtacatct taggaccaaa tattccagcc tttttccaaattctaacgat tctacatctg 5941 ccccttcatt caatatttat taagctYcta cYctatgtcaggcactgcct gagaaagaga 6001 aacaaagctt tctcacctgc agcttatatt ccaaaactaagttaataaat acataatacg 6061 tcatggggtg ataagcacaa tggagagaaa gaagggataaggattacgga tagaggttgc 6121 tataaggtga tcatggaaag cccctctgat gagatgacgtataccaggag atctgaagaa 6181 aggaaacaag ccatgtggat tgcggggtga gaggtagggtagagaaggga aaaccaagtg 6241 aggaatggca aaagcaaagg cctcgaggca aaacatacttggtgaggctc cctgaagaac 6301 aggtggaagt gtcataccac ttgtcattta aatgcaaattacactctgca gtcaggctca 6361 ctgccttccc cctcctttaa aaaaaaaaaN atccatccatccatcaattt tattctttga 6421 caaacttaag actcagttac tttttacttt ttataacaagtattcatcct gaaatgaaat 6481 tcctaaatac acttccttca taatactgta ctgaatacatataggctaaa tactccctac 6541 atagatttaa aaaaaaaatc agtataatgg cttatcagtgaaataaagga gaaatgagta 6601 atttctatca aatactacaa aatataaata cttgtacacaactacatttt aaaaagtagt 6661 caaaaactac tataactgat tagctgtgtg accttggggaagctatttaa atttcccata 6721 cttcagttta ctcacatgga aaatgagaat aatagtttctaccttacagg gttgttgtga 6781 ggattaaatt agtgcatata cataaggctc ttagaacagtaagtgctatc taagggtttg 6841 ctatgatttc tcttatgggg agaattacca caaaagtaacagtagcatga aaatgaatac 6901 aggggtgtta tgttagaaaa attttccttt ttcctgatatgatgaatgat tctttgaaag 6961 gtttcaaatg taacaaagtg ccaYcttcac aatatacactattgctgcat tactagaaaa 7021 tttggtgtgg attaaaacca tgtaaaaata tttaatacttatacgtaaaa agtagttggg 7081 ttctaggctc agagaattat aaatgcattt tgttttttcacctatgtgaa tagctgctgg 7141 gactttttgg aagttgtgtg ggacacacag caaacctccctggtgaaagg ctgtcccatg 7201 tatcccagga catctagcct ccctggcctc tgcYcaccaagcaccaggag tactccccag 7261 tcactgtccc ctgaaatttc ctgaatgttc cccagtgttaagaatcactg ttctattttt 7321 ttcaactgtc ttcaactttg actcctcatc cagggtggaggatctttgag gccagaaaag 7381 tatcttatta tgtccctgta tttctcaagg tagtgtactattgccacaaa aagattgttg 7441 gctgatgcag atgcaaaaca tcaatatttt tatactgattataggtgaca ggaaaggaca 7501 agggttttca ccctcagcaa aatcatcaaa aacatttaaaacagattaca tatagtagat 7561 aatactcctt gtcttaaaac attatgcaga aacacacatacagtttccct tccctctaaa 7621 atctgcagcc tcttctccct gctgaatgtg agtgatggtcccttcattca acagacttct 7681 atttggcaac tattatgtgc caggcattct aataggtgctggatgtgaaa ggatttaact 7741 tattcaaata agtccctaat ttgctgcaat aagggagattatttaatcat attgagtaaa 7801 aagtaccaag gaaagtttcc atatttctag ggaataagaaatcttttcaa aaatctctga 7861 gccaaattaa aattcatttc attttcccta atctccatttctaggagact agaatctcct 7921 tcctattgcc acggtagctg ctatctgcag agagaatctagataatttag aagagaaatt 7981 tgaatagagc cagagagata caattcttat agtcagacttggcttcacca aatttaatga 8041 agaaacttta ataagttaca acatttacta ggcacctactatgcacaaag aactttacat 8101 acctcatttc taatcttggc aacattctga taagataaatattatcccta ttttaacatg 8161 aaaagatgga ccttcttaca tattcaataa cttgcKccataggtgacagg attgaaatgc 8221 aaacccagtc cttgctctta atggtgtatg acagctcatcatataattag gctgatgcaa 8281 actctatgat aaacagtatg aatctaggag tggatttgtttatcctcaat ctttttgcta 8341 actttacagt gccttgaatt aaagaatcRt ggttactatcaccatggtca gatctctgga 8401 cagggtgagg aagcaggtgc ccacaggcag acagccctgccatatagtga gacacgctta 8461 tgtttgggag cgctatgtga acaacacaca gctgccctcatatgcagatg taatgatgac 8521 gacacattca gacaaggaac tgaggtccag aaaagttctgacaacacaac ttgagggaca 8581 gcgttacagc tagagctcag gtttcttatc tgtaatccactgctctcaaa tgcccaaatg 8641 attttatata catatatgca agtgtgtttg ttgaaatagaaaaaatagtt tcttggcctg 8701 gtgaagtgcc tcatgcctgt aatcccaaca ttttgggaggccaatgcggg aggattgctt 8761 gaggccagga ggtcgagacc agcctgggca acatagagaaaccaccgtct ctacaaaaaa 8821 tttaaaaatt agccaggtgt ggtggcatgt gcttgtagttccagatacta ctgaggtggg 8881 aggtgggagg tgctgaggtg ggaggatggc ttgaggccaggagtttaagg ctgcagtgag 8941 ctatgattgc accactgtac tccagcctgg atgacagagcaagaccttgt ctcaaaaaag 9001 aaaaaaaaaa ttcccataat gttcagttag ttctctttttcagacttttc ctagaaatac 9061 ccctcagaat ttctagaggc cacttccctg ctattccttgttttctcagt ccttaccaga 9121 tacccaagca attaacaatc tttttgaact atttcagtacatttcaggat atttgaaatt 9181 ctgctgatgg agctgaactg ctgatacatt aacacatgctgtcattattg actttgtaac 9241 agaggtcaag agagaaaaga tgcatttttg aatagggaaaaacaaaacta aattatgggg 9301 aaactaaggc tcagagaggt caagttgtct tgctcaaggtcacaaagcca gtagtaagta 9361 acagggccag gatttgaacc cagactgtct ggtttcagagtactctaaat tgccaaaaaa 9421 agaaaactat ttcaggcatt attttatatt tagcctagagcctacataat agatccgtta 9481 atataatcaa ttctaagctg tatattatat tactgtgtttgaaatattta agaaatattg 9541 tttaaaataa gtgtattagc tcttRgacct ctccaacataaggagttttc caggcagtag 9601 cactgtttgc agtaggactt acattattgg aaaatattttatcctataat caatctacat 9661 ttattttaca cctttcacct taaaattaaa agtcgtggtagttattaatc aagaagcaaa 9721 tctatataac gtggtctctc atatatccag tactgttcagcttgaacaaa tgcaaatcta 9781 atttttaaat gtcccttgaa gaattctgag gcaaagttaaacttcaagta ttaaacagaa 9841 tagccacaga aaactagtgt ttctcagcct ccctttgctttcaaatgcct taaaaatatt 9901 ttagaggcag acaataatct agaattgatt cataaccaatattttacata acagaatgtt 9961 acatgtattt tcatattata aaaactatac tttctttgattaatgctagt attctgaaaa 10021 catcgttagt gccagtaaat ccactaaatc tgtttgtatttactgccctc tagtgataat 10081 ggctctacat ttgattaaga aaaaactatt cctttcagtaaatgaaaaca tctcttgtaa 10141 gttttatgga gatttgagat taagaggaac tccaggctgcttttttaaaa ccaaataatc 10201 atctgaacaa gctgagaatc gttctctaaa tgatcttattttaatttttt tttaacaaaa 10261 aagtctcatt taaaatagta aacacctgca acatgtatggagaaaggaaa ccatttttgc 10321 taagaggctc cttgaagctg aaataacatg agactgatctgatagctgta tttatatttc 10381 tcttctacat gggctaactc tcttattaga tgttagcatagtattctgta attgttgata 10441 cagtttgaat tcatatatgg tagagacaga gttttgtataactatttagt caatttacct 10501 aaaaccagca gcagcagcat atctactaca gacttttgtttcactgaaga ataatcatga 10561 caataaatgg gtgcaaaaac ttcacataaa tgaagcctccacaaaaataa tcttgtatga 10621 aaaagtattg tctccttata gagaaaatta ggatctccaattcacttaaa ttggcctaaa 10681 tgaataccat aatcatcata aaatagttac taatgtcccctctgtacttc gtggtatgct 10741 ttgtggaaac aatttaaaca aagagaatat ctgtctcctaaagtaataat aactagtgta 10801 aataggcatg cttccagaaa tatataatct tttacacatatcactttaat atgtgcatct 10861 taaaataatt ttaagtaata aaatttgagc tataaaagagaaatgatatt cttttcaaca 10921 aagaatgctt gaacaattga gcatccactc ataaaacaagaaacttcaac ctttatcctg 10981 caccacacta acatctactt gaaatgaatc atagaactaaatgtaaaatc taaaactaaa 11041 gaacttctag aagaaaacac agtagaaagt agttgtgatcttgYgttagg caaagatttc 11101 ttaaaccaca aaagcatgaa gcataaaata aaaaatggtatactggactt taYcaaattt 11161 ctctttgaaa aaaactgtta agaaaaYaga acaacaagccacagacttga gaagggaata 11221 tgtacaaaat gcatatcaaa taaaggactt gtatccagaaattatttttt aaaaaatctt 11281 tacaacacaR taagaggaca atccagtgaa aagatggtcaaaagatttga acaaacaaac 11341 acttcataag aatcaaaaag atcaataagc atgtgaaaagatccaYgacc atcattagac 11401 attagggaaa caaaaagtaa aaccacagtg agatgccacaYtacatactt actagaatgt 11461 ataaaataca aaatacaaac aatacaaaat gctggcctggatgcagagca aattaaattc 11521 tcatacattg ctagtgagaa tgcaaaatgg tatagaaactttggaaaaag gtttgacagt 11581 ttcttataaa gttaaaaagt agagtgacca tatgacccagcaatcctact tctaagtatc 11641 tacccatgag aaatgaaaac atatgtctat ataaagacctatatgaaaat gtttatagca 11701 actctctata gcagaaaccc agaaacagtt cagatatcagtcaactggtg aatgggtaaa 11761 caaattgtgt ccataaaatg acatactgga aaaaaatgaaatactaaaaa gcaataaaat 11821 gaaacaaatt accaatagat gcaataacac aaaatgtattataagtgaaa cttataattc 11881 aactcaaaac tcaaaagtat tgtaagtgaa agaagccattcacaaaaggc tatatcttta 11941 atgtattatt cattctggca aggcaaaacc atagggacaatcagcactgg acgtggggtt 12001 tggggaatga ctataaaaag caagagaaaa ctttttgggtggtataaata tcctatatta 12061 tatagtacat atatattata attacacatt acctatatatctagattatg gtggtggtta 12121 tccaattgta cacatttgtg aaaatgcatt caactatacctctagaaaga gtaactctta 12181 tgtaaattat acctcaaaaa tctaactttg aaaaatattagctgatgtta tcaaattatt 12241 tcaattccag atttgcttaa ctttacaact ttgcccaaagataaatgcaa aacaaaaacc 12301 tcaggcatag ttatgtatga taaaacacca tttttaccctttcaaagagc tccaccatta 12361 tttcccagaa aaYaggcata cagttatcca aggtccacattagctttaat gatacaacat 12421 tcagtgaatt cccagttgtg aaaagccttt aggttggcagggcgctgtgg ctcatgcctg 12481 taatcctagc actttaggag gccgaggcag gcagattgcctgagctcagg agtttgagat 12541 cagcctcggc aatatggtga aaccctgtct ctactaaaaatacaaaaaat tagcagggtg 12601 tKgtggtgca ctcatgtaat cccagctact cgggaggctgaggcaggaga attgcttgag 12661 cccgggaggc agaagttgca gtgagctgag atcgcgccactgcactccag ccggggtgac 12721 agagattctg tctccaaaaa taaaataata agagaagcctttaggtgata aaataatagt 12781 caaagtattc ttttaagtat tattatttta tttttttttaattctttgta ataaaaagtt 12841 ccataaagta aaatattgtc cttttttgtt ttatttttatttgtatcaat tcaaggggac 12901 aggtgcagtt tgttacctgg acctgatttt taaataaatcaaactcagtt tagttatttc 12961 atggtgatcc tgggagactg tttgtcagtc atcatcgaatagcgaatgag tatcctttgt 13021 atgctcattc taggctgtaa gtaccatgtc agctgatggagacacaaaaa tccaataaag 13081 taggtatagc tgggggaggt tccaagatgg ccaaataggaacagctccag tctactgctc 13141 ccagcatgag tgatgcagaa gacgggtgat ttctgcatttccaactgagc tttgaaaaga 13201 gtagtggatc tcccagcatg aagtttgaga tctgagaatggacacactgc ttcctcaagc 13261 gggtccctga cccccgagta gcctaactgg gaggcacctcccagtagggg ccaactgaca 13321 cctcatacag ccagatgccc ctctgagatg aagcttccagaggaaggatc aggtagctac 13381 atttgccgtt ctgcaatatt tgcggttcgg cagcctctgctggtgatacc caggcaaaca 13441 gggtctggag tggacctcca gcaaactcga acagacccacagctgagggt cctgactgtt 13501 agaaggaaaa ctaacaaaca gaaagggcat ccacaccaaaaccccatatg tacgtcacca 13561 tcatcaaaga ccaaaggtag ataaaaccac aaagatggggagaaaccaga gcagaaaagc 13621 tgaaagttct aaaaatcaga gctcctcttc tcctccaaaggaatgcagct cctcaccagc 13681 aacggaacaa agctggacag agaatgactt tgacaagctgagagaagaag gcttcagacg 13741 atgggtaata aacttttccg agStaaagga ggatgttcaaacccatcgca aagaagctaa 13801 aaaccttgaa aaaagattag acaaatggct aactagaataaccagcatag agaagacctt 13861 aaatgacctg atggagctga aaaccatggc atgagaactacatgatgcat tcacaagttt 13921 cagtagccaa ttccatcaac tggaagaaag ggtatcagtgattgaagatg aaatgaatga 13981 aatgaatgaa atgaagcaag aagagaagtt tagagaaaaaagagtaaaaa taaatgaaca 14041 aagcctccaa gaaatatggg actatgtgaa aagaccaaatctacatctga ttggtgtacc 14101 tgaaagtgat gggagaatgg aaccaagttg gaaaacactctgcaggatat tatccaggag 14161 aaattcccca acctagcaag gcaggccaac attcaaattccagaaataca gagaatgcca 14221 cgaagatact cctcaagaag agcaactcca agacacataattgtcagatt caccaaagtt 14281 gaaatgaagg aaaaaatgtt aagggcagcc agagagaaaggtcgggttac ccacaaaggg 14341 aagcccatca gacttacagc tgatctctca gcagaaactctataagccag aagagagtgg 14401 gggccaatat tcaacattot taaaggaaag aattttcaactcagaatttc atacccagcc 14461 aaactaagct tcacaggtga aggagaaata aaatcctttacagacaagca aatgctgaga 14521 ttttgtcacc accaggcctg ccttacaaga gctcctgaaggaagcactaa acatggaaag 14581 gaacaaccag taccagctac tgcaaaaaca tgccaaattgtaaagaccat caaggctagg 14641 aagaaactgc atcaactaat gggcaaaata accagctaacatcataaaga caggatcaaa 14701 ttcccacata acaatattaa ccttatatgt aaatgggctaaatgttccaa ttaaaaaaca 14761 cagactagca aattggataa agagtcaaga cccatcagtgcgctgtattc aggagaccca 14821 tctcacatgc agagagacac ataggctcaa aataaagggatggaggaaga tctaccaagc 14881 aaatagaaaa caaaaaaaag caggggttgc aatcctagtctctgataaaa cagactttaa 14941 accaacaaag atcagaagag acaaagaagg ccattacaaaatggtaaagg gatcaattca 15001 acaagaagag ctaactatcc taaatatata tgcacccaatacaggagcac ccagattcat 15061 aaagcaagtc cttagagacc tactaagaga cttagacttctacacaataa taatgggaga 15121 ctttaacacc ccactgtcaa cattagacag atcaacgagacagaaaatta acaaggatat 15181 ccaggaattg aactcagctc tgcaacaagc ggacctaatagacatctaca gaactctcca 15241 ccccaaatca acagaacata cattcttctc agcaccacatcacagttatt ccaaaactga 15301 ccacatagtt ggaagtaaag cactcctcag caaatgtaatagaacagaaa ttataacaaa 15361 ttttctctca gaccacagtg caaagtagaa gttgggattaagaaactcat tcaaaaccac 15421 tcaactacat ggaaactgaa caacctgctc ctgaatgactactgggtaca taatgcgatg 15481 aaggcagaaa taaagatgtt ctttgaaacc aatgagaacaaagacacaac atactagaat 15541 ctctgtgaca catttaaagc agtgtgtaga gggaaatttatagcactaag tgcccacaag 15601 agaaagcagg aaagatctaa aattgacacc ctaacatcacaattaaacga actagaaaag 15661 caagagcaaa tacattcaaa agctagcaga aggcaagaaataactaagat cagagcagaa 15721 ctgaaggaga tagagacaca aaaaaacctt caaaaaaatcaatgagtcca ggagctggtt 15781 ttttgaaaag atcaacaaaa ttgatagacc actagcaagactaataaaga agaaatgaga 15841 gaagaatcaa atagatgcaa taaaaaatga taaaggggataccaccacca atcccccaga 15901 gatacaaact accatcagag aataacacct ctacacaaataaactagaaa atctagaaga 15961 aatggataaa ttcctggaca catgcaccct cccaagactaaatcaggaag aagttgaatc 16021 cctgaataga ccaataacag gttctgaaat taaggcaataattaagagcc taccaaccaa 16081 aaaaagtcca ggaccagaca gattcacagc tgaattctaccagaggtaca aagaggagtt 16141 ggtaacattc cttctgaaac tattccaatc aatagaaaaagagggaatcc tccctaactc 16201 atttgatgag gccagcatca tcctgatacc aatgcctggcggagacacaa caaaaaaaag 16261 agaattttag accaacatcc atgatgaaca tcgatgcaaaaatccttaat aaaatactgg 16321 caaaccaaat ccagcagcac atcaaaaagc ttgtccaccctaatcaagtg ggcttcatcc 16381 ctgggatgca aggctgcttc aacatatgca aatcaataaacgtaatccat catataaaca 16441 gaaccaaaga caaaaaccac atgattatct caatagatgcagaaaaggcc tttgacaaaa 16501 ttcaacagga cttcatgcta aaaactctca ataaattcggtattgatggg atgtatctca 16561 aaatagtaag agctatttat gacaaaccca cagccaatatcatactgaat gggcaaaaac 16621 tggaagcatt ccctttgaac actggcacaa gacagggatgccttctctca ccgctcctat 16681 tcaacatagt gttgaaagtt ctggccaggg caatcaggcaggagaaagaa acaaagggta 16741 ttcaattagg aaaagaggaa gtcaaattgt ccctgtttgcagatgacatg attatatatt 16801 tagaaaaccc catcatctca gcccaaaatc tccttaagctgataaacaac ttcagcaaag 16861 tctcaagata caaaaccaat gtgcaaaaat cacaagcattcctatacacc aataacagac 16921 aaacagccaa atcatgagtg aacacccatt cacaattacttcaaagagaa taaaatacca 16981 aggaatccaa cttacaaggg atgtgaagga ccttttcagggagaattaca aaccactgct 17041 caacaaaata aaagaggaca caaagcttat agataggaagaatcaatatc atgaaaatgg 17101 ccatactgcc caaggtaatt tatagattca atgccatccccatcaatcta ccaatgactt 17161 tcttcacaga attggaaaaa ctactttaaa gttcatatggaaccaaaaaa gagcccgcat 17221 tgccaagaca atcctaagca aaaagaacaa agctggaggcatcatgctac ctgacttcaa 17281 actatactac aaggctacag taaccaaaat agcatggtactggtacaaga acagagatat 17341 aggccaatgg aacagaatag agccctcaga aataataccacatgtctaca accatctgat 17401 ctttgacaaa cctgacaaaa acaagaaatg gggaaaggattccctattta ataaatggtg 17461 ctgggaaaac tggctagcca tatgtagaaa gctgaaactggatcccttcc ttacacttta 17521 tacaaaaatt aattcaagac ggattaaaga cttacatgttagtcctaaaa ccataaaaac 17581 cctagaagaa aacctaagca ataccattca ggacataggcatgggcaagg acttcatgtc 17641 tgaaacacca aaagcaatgg caacaagagc caaaattgacaaatgggatc taattaaact 17701 aaagagcttc tgcacagcaa aagaaactac catcagagtgaacaggcaac ctacagaatg 17761 ggagaaaatt tttgctatct atccatctga caaagggctaatatccagaa tctacaaaga 17821 acttaaacaa atttacaaga aaaaatcaaa caaccccatcaaaaagtggg tgaagtatat 17881 gaacagacac ttctcaaaag aagacattta tgcagccaacagacacgtga aaaaatgctc 17941 atcatgactg gccatcagag aaatgcaaat caaaaccacaatgagatacc atctcacacc 18001 agttagaatg gcaatcatta aaaagtcagg aaacaacaggtgctggagag gatgtgaaga 18061 aataggaaca cttttacact gttggtggga ctgtaaagtagttcaaccat tgtggaagac 18121 agtgtggcaa ttcctcaaag gtctagaact agaaataccatttgacccag ccatcccatt 18181 actggatata tatccaaagg attataaatc atgctgctataaagacacat tcacatgtat 18241 gtttactgca gcactattca ggatagcaaa gacttggaaccaacccaaat gtccaccaat 18301 gatagactgg attaagaaaa tgtggcacat acacaccatggaatactatg cagccataaa 18361 aaaggatgag ttcatcatgt cctttgtagg gacatggatgaaactggaag ccatcattct 18421 gagcaaactg ttgcaaggac agaaaaccaa acaccgcatgttctcactca taggtgggaa 18481 ttgaacaatg agaacacttg gacacaggat gggggaacatcacacactgg ggcctgttgt 18541 ggggtgaggg gagtgggagg gatagcatta ggagatatacctaatgtaaa tgacgagtta 18601 atgggtgcag cacaccaaca tggcacatgt atacatatgtaacaaacctg cacattgtgc 18661 acatgtaccc tagaacttaa agtgtaataa aaaaatWaaaaaagtagata gtcccaaccg 18721 ctgtattctt tttgaatcct tttgccactg tttcatatcacagttttttt ttttttctga 18781 gccctagatt atcagtctgt tctttttttt tttttttttttttaatgtag tccataggaa 18841 tctgctgcct cacRgtcagt atcgtgcccc ttaccccaattctaatatga caggtttttg 18901 tctaagacag catttcctag aatgtgttgt aatgtacagcagcttcatgt gataattctc 18961 aaagactccc actgtcaaat gttagcatat tcaaggctctgccaggacct gtaatacata 19021 ttctttcttt attctgttta acttagcatt tctgaaacctatcttactac tgaactcttg 19081 acatttatta acaaacaaat acaaacRaaa ataccagggttccaagtaaa attettatat 19141 aaagcaaagg gctttaaaca tcagagtttt ttagaatggttattcactca tgcactcaac 19201 aaacacttat tacatagtat gtgccagcat aggggatacagtaataaaca aaacagacaa 19261 gttccctgtt catgtaaacc ttggacttat ggggcaaaaagacaataaaY aagcagacaa 19321 aaaaaaatta caaacactct caatgaagga aaagagggtaacatcgtaca ttgatggggg 19381 actataaaat taagtagtaa ggaaaagctg cacttgagaaggtggtattt gggaagagac 19441 tcgaatgcct agaataagcc aaccagattt ggggcacagcactctcaaca aaggctgagt 19501 aacacagcca gtctgaggga ggacaggtct acggaatgagagccgaggca agtaaaggca 19561 acatcacgtg ggtcctgagg gtatgagcct tccagagggtgtgaggatac cattagaagg 19621 ttctgagcag ggaatgatag gatgtgattt acattttgtaaagctgtctc tgtagaggat 19681 agtttgtaaa agtgcttgag gcaaaactga caagacttgaagacttgcac aacagaactg 19741 aagaaatgag aagaatcaac tcctagactt ctgggatgatcaatgtctag gatggacatt 19801 ggtgccattt gcaagatgga gaagaccggg ggaggtacggatttaaagga gggtatgtaa 19861 ggagaaagaa gaatcctatg agacaagacc agaacatgtaaatacatgaa gttttggatt 19921 tcttactctt caaagacatt ttacctcctg tccccgaagttcttcacaga gttcaggata 19981 agaaaagctg ccagacagtt gagttttgaa taagtagtaactttcaaaca cttgaaagac 20041 attatattag aatagactac atccatcaaa ataNttaaaaatgtttttag aatagtccaa 20101 taatttggaa tatgaaagcc taagaaactt ccttatccaaggccRcataa tataaccaaa 20161 attagaattt agtactccta gttggcaata tttttatgaaattactgttt tactaagaca 20221 ttttagaaat gaagacttta ctgaaggcaa taataagtgcaattaaaagt ggtacaataa 20281 Kaagacttct ttttcatttg caacaggtta gtagtgtatgagaattatga gatgtaaaat 20341 agtgtagagc ataggcttct ctagttggct tcatgtgagttcgaatcttg gccctgcctc 20401 tcttaccagc aatgaaatat gcagcatttt acttaaggatactgggttgt atggagagaa 20461 tcagagttaa ggttggtgat ccagcagtgt catgtgtcaggagctaataa attgtattgt 20521 gaccagaaac actgaaactt gttataagga aagcaatctaattaaaacag ctgcattaaa 20581 atttctacaa tatcttccta acagcttatg ctatcaacagtttgtaaact atcagaatca 20641 cggatactgg ctgagaatgc ttttggagat ttcaaattggcataattttc tcagaatgca 20701 ctgatctgac aaatgtacag acaggcagac aggaatactaccactcttca tgaataactg 20761 tctgcctctc ctggatttct tccatgttgt gtaagtctattacactttgg gtgaatgtag 20821 ctttagtaca ttgagttaat atcattcttg caaagaagctctttataaat tccttaaact 20881 taatttgact ctggacacaa acaaatgaga acaaaatacagctcaaggat taagatcctt 20941 aaccaaatat gtaaaaaaca cgaaaagcct tacaaacagtgatcaacagt taaacatgcg 21001 ctatcacctt cccctaacag ctagggaagc atgtatatgaatggaaeata tgaacttcca 21061 tttggtgtac taagaaaaca catcacgcag atggcttttatagcttccca aataaggttc 21121 taataattca caaaagtgtt gttccctaaa acccataataaacaataata aaacctaatg 21181 tttattgagg gtctactatg tgtcaaggag agtcctatgtactctcctgg aattacttca 21241 tttatttcta ataataacct ttaatgagca ctgtcactcocgtttcacag atgagcctta 21301 aggtcttagc tatcaacttt gcagacaaca cccaagtaaaatcctgcccc ctcttttttt 21361 tttttttttt aaccagaaat ccagtcctga caactgaaagcttatagggc atttccgctt 21421 agatacatag ccttacctaa attatatgtc taaaaacttaactgtcatat tgtccagccc 21481 tcccccaata cccaactggc ttctcttcat gattttccaactataattct gtccattatt 21541 caggtttaga atttcatgct ttcttctggc tgtggtcctgccaagtctta tgtatgctgt 21601 cacccttctc tgtgcaatct ttctcctact cattcctctcttttcatcac tatcctattt 21661 caggccctta tcactcttac acctaattcc ctaacggtttcttcttgctt tcagtacaat 21721 ttccccttga tataatctat cctgcacact accaccagatataaccttct aaagtcacct 21781 tattttagtt tcttcataat attttcctta tagtcaaatgtaagggttct tcatgaatta 21841 taggacaatg tgcaaatggc ttgacctggc atttaagacagtctgcagta tggcctcaat 21901 ctacatttct cacacctgcc actggtccta taatcacatcctctgttcca gagaggctca 21961 ctcctccaga atgcccacta ccctcctctc cagcaaacatacaataaagc gaatccacat 22021 gaatacccca tcctaccctg ccttatccag cccctggaataccacctctt ccctattaga 22081 tgcctatata aatcctcagt attcaagatc cagcttaaggagcacttttt ctgggaccaa 22141 tcattctggt cctctgtatt gaatactcga actcctctacaggacaggaa cataatgtat 22201 tattacccta tcattcatta acacacaaca ggcttacattatatggctgg ttgtattttc 22261 agaggtatgt ctatatgttt cgctatctat ctatctatctatctatctat ctatctatct 22321 atctatgtgt ttgaatacag atacataaaa atctcatttcacctataaga acctctggag 22381 cagatacatg tcatacaata attgggctaa aacaaaaatccctcagtgaa aacctaaaga 22441 ctgggttgtg gttgtttgca gccaaaacgt acaaaatgaaaacctcctaa cgatttattt 22501 tccacatgaa attagtacca gatctagaga aaaatggacaaggatcaaat acaaataata 22561 aagtcatcac caaactaaaa atttcattca aaagtgatgatgttggccgg gtgtggtgac 22621 tcacacctgt aatcccagca ctttggaaag ctgaggtgggcagatcactt gaggccagga 22681 gtttgagacc agcctggcca acatggtgaa accccatctctactaaaaac acaaaaaaca 22741 gctgggcgtg gtcatgcacc tgttataccc actactcaggaggctgaggc aggagaatca 22801 cttgaatcca ggaggtggag gtggcagtga gccgagattgtgctactgca ctccagcttg 22861 ggcaacagag caagactccg tctcagaaaa ttaaaaaaaaaaaagtgatg atgtcagtaa 22921 agacccaaat cagggtcata tgtttaacac cagaaagtttgtatttgcta caagacagaa 22981 accttgacaa aataactttt ctgtgctttg ttacttcatctgaatgaaga aggtaaacca 23041 gttgatctca caaatctctt tctaagggct tctggaattttttttttttt ttaaacaata 23101 aaaggaaaga gacttcYctt aagcagaaca aagtccgactcctctgtaaa gtggctgaca 23161 gcaagtggaa aatggaagaa gaacaattac ctgcaaaatgactcattccc ttgtaacaac 23221 tacactacta gggaattcct tctcttaccg gctgtggctagcgtcagact catgacaaag 23281 acctcatcct ggggctagaa ctaggtttag ggttttctcattcattcctc tgtttctcag 23341 ctgcattttc atttttaatc tgtgcaatga ttacagaggcctatgtttgg ttttcatttc 23401 tagtttcccc aggaagagac aactactatc ttgatattgattaacacttg aaacttctgt 23461 taatcacaat ttcagtattt cagggaatca gagaaatcatttggaagagc cttgaaggtc 23521 atccagggtg agagaaaagc aaagcacaag caacagaccaatgtttagac acagtgaagt 23581 gaagRcctac aggagaccct ctcaacttca ataagagtcttttttatggc tgtcttcata 23641 ttctttcaaa gaaatcataa taaaggagtt agtgagagtgtaaataaaaa ataggctatc 23701 atagagtgct gggtaactga gctaaaacta aagtaaacaaactcagtggg ccatcacata 23761 tgcaagctgg taaggtctca taaatcacca tgactgggtattgggatttc aaaggcacac 23821 tctgtaagct ttctcccagc cctgttgatt caagctccaattgcactggg ttacttatat 23881 ggcagcatat aaagttgacc ttttaYgttt aaaaatagttccattcctca tccctcctaa 23941 ggataggcca cagtacctat catatgtccc caccacactacataatttat gtaagttttc 24001 acttaatttt caacacaatc ctttgaggta gattatgttagtcctSattt acagggaaaa 24061 cagaggctca gggaggccaa atttatttat ttatccaaggttgcaactct actgtagggc 24121 agatttatat ccagaagagg aattgcaaag ctcctcagaatttaggagat ttaaaatgga 24181 atcaagataa aggttcacat ttttcatctt ctattccattttactcttcc agcatctaat 24241 aataacctat gatatattag aacaaatcag cccatacttattttgacagt ctcttttcac 24301 cccatttcag tttgtacctt gctgtctcag agcccatcaaccacctttcc tcactcccag 24361 gcagccggtc cttcagggga tggcttggca gttggcccaccatcctctgc taccagggca 24421 tgactcatgg aggcaacagg tgaagctgaY gccaactattagtgctaaaa aagactcaag 24481 cctcatgaaa tataccatac ataaatcgct ctccagtcagctctacccct ccctccattt 24541 ctacagctga ctgtctgttc ttccttaaac atttacatccgccactgacc tgtctgggat 24601 ttctttttta cttcaaggYt ctcctaaagg ttcagtMcaaccaggtttca ccaaatgaac 24661 cctgcggtgg aaaggggcag caacgggcat tggtcagtttgcctccagcc aaggaaagtc 24721 tgactcccct ccttctccca ctcttctctt tctctgcctcacactgccta atacgtcttt 24781 cctctttctc tctttctttc cttatatctt cattttccaccctattcaga agtgaaactt 24841 cccttgccac tttgccccca ctaagaaaac agacctccaacacaacctct taaYaggcta 24901 attttaaaat catgaacctg cttacacctt ccgtgagtttatcctatatc ttctctatct 24961 gctgtctgct tcccactaag gttttatccc tccatcccacctccaaatcc tttacagctc 25021 agattctcaS atctgcttta gtcacttggt ctcctcatcccactgaccct attatccttc 25081 tgtgcacatg ttagagggca catgctcctc ctaggaactagggccaggtc agttatgctc 25141 ccatcctgac atgacctaga gaacaaaaaa ggcaaaacgaggtactgtag gattcctctc 25201 taggaattat actttgctga aattgctctt gctattttggaatctagtgc tcactaaata 25261 catttttaaa atattatttg gatatatttt gaaaatacaaccagaggtcg ggcacagtgg 25321 cttacgcctg taatcccaac actttggcgg gccaaggtgggcagattacg aggtcaggag 25381 ttcgagacca gcctggccaa catagtgaaa ccctgtctctactaaaaata caaaaaatta 25441 gccaggcgtg gtggcaggcg cctgtaatcc cagctgcttgggaagctgag gcaggagaat 25501 tgcttgaacc tgggaggaga aggttgcagt gagcctagatcacgccattg cactccagtc 25561 caggcaacag tgcaagactc taactcaagg aaaaaaaaaaaagagagaga gagagaaaat 25621 acaaccagag aataaacact tgttcttcta tctctccatcttccccacca atacaacatc 25681 cagaaaatag tcatctttac acttatttag aagcacatagatcttataaa aStttaactg 25741 agcattaggt tttcatcttt aaaaatattc tgaggctgggtgcactggct catgcacttt 25801 gggaggccaa ggcaggagga tcacttgagc ccaggagtttgagaccagcc tgggtaacat 25861 agtgagaccc tgtctctaca aaaaaaaaaa aaaaaagttaaattaaatta aaaaacagaa 25921 aagccggtct ggtggcacat gcctgtggct ccatccactcaggaggctga ggtgggagga 25981 tggcttgagc cagggagatc aaggctgcag taagccatgatggcgccact gcactccagc 26041 ctggttgaca aggtgagaac ttgtctccaa aaacaaaacaaaacaaaaaa cgttttgaca 26101 tgtagaatac tgtttctggg actaaattag agatttaaggtttgaggtat gagaaaatac 26161 ctggaatcat gccttacatt agaaggtaag gtaaatactggtgtttccca atgagttgta 26221 gtcagattgt tcatatgtat caaacagaca atatactgaaatatttaaaa ataaggctaa 26281 atagcaacaa ataatttttc ccatgaattc tacttttttttttttttttt ttgagatgga 26341 gtctcactca gtcgcccaga ctggagtgca gtggcgccatctccattcac tgcaacctct 26401 acctcctggg ttcaagtgat tctcctgctc agcctcccgagtagctgaga taacagacat 26461 gcaccaccac acctggctaa tttttgtatt tttagtacaggccaggtttt gccatgttgg 26521 ccaggctggt ctcaaacttc tggcctcatg tgatcgacctcccaaagtgc tgggattaca 26581 ggtgtgagcc accatgccca gctgaattct actttttttttttttttttt gagacagagt 26641 cttgctctgt cacccaggct ggagtgcagt ggcacgatcttggctcactg caacctctgc 26701 ctcctgggtt caagtgattc tcctgctcag cctcccgagtagctgagatt acagacatgc 26761 accatcacac ctggctaatt tttgtatttt taggagagacgggatttcac catgttggcc 26821 agctagtctc taactcctga ccgcgtgatc tgctctcctctgcctcccaa agtgctggga 26881 ttacaggctt gaggcaccac gcccggctgc tgaaattctacttttgaaag aacaaattcc 26941 ccttctgtag ggactgatga ttcagtctgt aatgatgcattctcttcatc ttcttttttt 27001 gtcttttctt ctatatatcc tttaggcatt ctactgtattaacactagat agaaaaatta 27061 agatttaggc ccaaacatta tcttctggca taaagtaaactaatcKtttc ctgatgtaca 27121 ataaataaac agacatattt ctagtagaag attccttatgatttatcaaa acccaattat 27181 cacttaccct tatggagatc ttagatctta gaaaccatactgacaatata agattaagaa 27241 aacaacagaa aatcaaaata tataccaagg ataatgttaataggtttcat ccttatcaca 27301 gcatactgtt gaaacttact tgaaatattt ttttaaaaaagatacaagag gacaagtgtt 27361 tagtttttaa caagggaggg gactagtcaa caaaYattgattacctaatt tatgaacagc 27421 tctggccaaa gtggtcaaaa aaaaaaaatt aggactttagttttcctaaa cctatcttaa 27481 tttttaacag gattatagct ttgttcaatc tttacagtctttagcctctt ttgtgaaatg 27541 ggtttccttg taaataataa aatgacacct atatatatttaaagcactca tgaaataatt 27601 ttatctcttt attaaaaact gtgataaccc ttccaattttgaaatttatt gtcttcctaa 27661 attcttgcta caaagcctat cattaatatt gcagttcattttttccctaa agattgaaaa 27721 aaacactaac aaatataaag tctttatggt caaagaatgtactcctttta taaaacttgg 27781 agttgatgta aaggcacaga caacacaagt taccttaataataaatgcac ttttctttgt 27841 aattttgggt aattcagtgt gatttgtaat tcttttaaaagacacacaat cacttgtatt 27901 tttgcatggt tctttttttc ctaatttccc actatagtgaaatatctgtt tttccttctc 27961 tattgggttt ggattggaat gatcaggtag aattaaatgtaaaataacaa aaggaaaata 28021 ctttatagat cttctcaaaa tactttgaag ggtataatctctttaagatg gctttattca 28081 tatttaagcc aaaaacaaaa agcaaccaaa acactactgtttatacaaaa agcagcttca 28141 aaatcagcca gttattttct gaaatggaga tggcagacattagcctgcac ttactccaat 28201 atggcagccg catctttgcc tctttgctat ggacacatccccagcaggcc tgtacttect 28261 actctccaga cctgtttatg acaggccaag gtgtttcctattatggtgcc tatggctgca 28321 cagtgcactg cttgtcagta ataaaagcca gaggctgtccctatagggat tacagtgcag 26381 cagaccagag atgctgatgg gaatgcaagc atgccctaggcaaacattgc agaatactct 28441 ctgacaggca aaagccaaca aacacatcct ttcttcccagactagcaggg aaagtattca 28501 gttatgtcat tttcacttgg aacaaactaa taagaagaatgttattgcta aaataggtag 28561 agatgagaaa gagcagttta tgaccatgaa tatggcctctatggtttttg cctgtgcact 28621 acaaactttt aatttaaacg agatgagatt gatctagcatatactatgat aaaatttctg 28681 agacatgagt atctaaaagc agaactgaga ttaagtgagaaatgctcagg attcctgtca 28741 ctgaaagaaa aagaaaaaaa cctttcttga aaggcacaaagtagaatgct cagactctcc 28801 atctaccaat acaaggtaaa aaacagcatt tgcattaaccaacatcatgc attcttgtac 28861 taatttatgt gactaactac aaaatgttat attgacactcactgtgtagg gtgtcaggat 28921 ggccaagtgg tctaaggcac cagacactca ctgggttgggctaaaaacac Ygtagaagac 28981 ctcatcagaa caataaaaca gagacacaaa atattgtactaagagttaag agaattacaa 29041 ataaaagttc tgtggaaagt caaaggtatc caattaggaaagcctgaggt gggagacata 29101 ggacacccca gatataggat gggaagaaga tgggattaaaaaaaaaggct ttataaagga 29161 tgtaacattc cagataggcc ttaaaagatg gcttcgatcttttaaataaa actttttgtt 29221 ttagaacaga tctagattta cagaaaactt gtgtagacattacagataat tcctatatac 29281 tctacactgt ttcccatttt taatgtctga ctttagtatggcacatttgt cacaattatt 29341 gaaccaatac tgatacatta ttattaaaca aagtccacacttcattcaga tttccttagt 29401 tttcacgtaa tttccttttt tctgttctgg atcccatccaggataccaca taatatttag 29461 tcctcatgtc tcctgaggct cttcttggct gtaggtatctcaggttttcc ttgtttttga 29521 taaccttgac agttttgagg agtactggtc aaatactggtcaagtttcga gggtagaata 29581 tccctcaact gagatctgtc tggtatttgc ctcatgattacactggggtt atatgttttc 29641 agtagggaaa ccacagatta aaagtgccat tttcatcagatcatatcgag ggtacatgtt 29701 atcacttatc actgtcaatg ttgaccttga taacctggctgaagtagtga taggWttctc 29761 cactacagtt tctttttttt ccctttctac actgtactgtttagaaggaa gtcaatatgc 29821 acagctcaca cttacggtgt agggggttat gttctccctccttgagagta gagtatctat 29881 agaagttatt gggaattccg cacaggagga gatttgtctattttccccca cttaggtatt 29941 tatttaataa attgtatcaa tatgggttta tggatatttattttaccctt tgggatataa 30001 tccaatacta tattattttc ttgctcaaat tgttctagctttggccattg ggagctcttt 30061 cagtttgctc ttgcaactct tcagtatatt cccacgggtgtagggttcca tttgtttgag 30121 caggtcctta ctttttggca ctacaagatg ctccaggctcatcttacata tttccttcct 30181 gagtccctag aaatcagcta ttttttttca aagagtcttggttcctttta ccagagaatg 30241 gtattagaaa ccaaaatgag ggtgctaagt gtgctcattgctactggggt atggtagctt 30301 ctagaccctc tcggctgata gagcaaggaa atatttgtgtgtatactaga tggtaagatg 30361 ttgagaggta gaatggagag ttaccttctt aatatacttttcttaatatt aaatattaat 30421 aagatattgt gtgcctgggt tactttcagt cttatgcagaggaagaacgt atgtacattc 30481 atttatgaaa aacactggct agactattct tctgcactgcattatgcatt tctgctagaa 30541 aaagctcaga gattttcaga tattctgaca aaaatctcaggcttgcttga tgagctatac 30601 tgtaaaatta cagaaggaaa caaagggcaa actccttgaaggatgaaaat aaaggaataa 30661 aacatataaa acaccaccaa aaaaagtgat taatgggggagaagagtaaa taaaacatta 30721 taatagtcct ctttggttag tatcaatatt attaggtcactttatcaaaa ggatagaaat 30781 caggatggca gacacccttc tctaaccctc accccccaaaataataaaat aaaaactaat 30841 gttattcctt cacatatgga aatgaatgag ttctactgagtatctagtat gattcctgac 30901 tcagtcgaga caattcccat cttttaattc attaaatcaccacagcaact atttaacaga 30961 tgagaaaact gaagcacaga aggcaacRtg gcccaatttacgtttggtaa aaggcagaga 31021 atgaatccaa ttcaggcagg ctgtctatag tagggtgctctaaatccacg gaccattctg 31081 cctaaggaat gaatatttta tgaattggaa tatttctgctgaacgaattt gccattttct 31141 cctctagtgg agaaatccaa ctgagggtaa agaaggaaaaatgttgattg tttaaaacaa 31201 taaattagtc tctaaattgt tcttgcagct gatttagactagtgtgtcca aaagtatgcc 31261 cagtgttagt aaatggtagg caaaatgctt taaggtggtgcaagatgaat gttttctcat 31321 tttgttattc attaatttta gaaaaaatac agaaaaaatataacttgaac atcaaaccta 31381 tgatttcatg tataccatta cttaagatga ggcacttgggtgagtattta gatttaacaa 31441 taagttgctc ttagcatttc tatactttga caataaattatccaaaaaag aaattaagaa 31501 aataatccca tttgcaatag catcaaaaaa atacttaggagcagccaggc gtggtggctc 31561 actcctgtaa tcccagcact ttgggaggcc gaggcgggcagatcatgagg tcaggagatc 31621 gagaccatcc tggctaacac agtgaaaccc catctctacttaaaaataca aaaaaaaaaa 31681 aaggcaggtg tggtggcagg cgcctgtagt cccagctaattgggaggctg aggcaggaga 31741 acggcgtgaa cctgggaggt ggagcttgca gtaagccgagatcgcgccac tgcactctag 31801 cctgggcaac agagcgagac tccatcMcaa aaaaaaaaaaaaNaaaaaac ttaggagcaa 31861 atctaaccaa caaggtgaaa gatctataca ctggaaactacaagacattg atttaaacaa 31921 ctgaagaaga cagaaataaa tgaaaagata tcccatgttcatgcactgaa agaattagta 31981 atgttaaaat gtccacacta tccaaagcca tctatagattcaatgcagtt cctctattga 32041 ttcaatgcaa ttcctttcaa aattccaatg tctttttttttcacagaaat agagaaaaaa 32101 atcttaaaat ttatatgaac caaaaaaaga cctaatagccaaagcaatct tgaacaaaaa 32161 gaacaaagct gcaggcatca tattccctca tttcaaaatatattataaag ctactgtaat 32221 caaaacagca tgttattgac ataaaaacaa acatcaaccaatggaacagg atagaaagcc 32281 cagaagtaaa tccaagtatt tacagtcaat tgatttttgacaaagggtcc aagaacacac 32341 aatggagaga ggacagtctc ttcaaatagt gttgggaaaactggatgtcc acctgcagaa 32401 gaaagaaact gtactcttat ctcacactcc atataaaaatcaactcaaaa gagatataaa 32461 ggcttaaaca taagacctga aactgtaaag ctactagaagaaaatataag ggaaaatctc 32521 cacaacaatg gtctgggcaa tgatttcttg gataggactccaaaagcaca ggcaactaaa 32581 acaaaaatag acaaatagga ctacattaaa ctaaaaagtacctgcacaag caaccaaata 32641 atagagtgaa aagacaaccc atggatcagg agaaaatatctgcaaaccat acatctgata 32701 agaggctaat attcaaaata tataaaaaat tcaaacaaccaaatagtaag aaaacaaatc 32761 gattaaaatt agRcaaagga tctgaaYaga cacactttcctcaaaaaaga aatatgaatg 32821 gccaacagag atacaaaaaa aaaaaaaaaa aaaaaaaacaatgttcccca gccctgcaca 32881 gtggctcatg cctgtaatcc cagtgctttg ggaggctgaggcaggagatc acttgaggcc 32941 aggaattcta gacaagcctg ggcaaaatag caagaccctctctatacgaa aaaataaaaa 33001 gccaggtatg gtggcacaca cctgtagtcc tggctacttgggaggctgag gcaggatcat 33061 tgagcccgag aatttgaggt tacagtgaga catgatcaYgccactgcact ccagcctgaa 33121 caaaagagca agaccctgtc tcaaaaacaa acgaaaactcaacaatatct ctaattctca 33181 gggaaatgca attaaaacca caatgagcta tcacctcatgcctgttagaa tgactatttt 33241 cctttttttt ctcttcaact tttaagttca ggggtacatgtgcaggatgt gcaggatgtg 33301 caggtttgtt acataggtaa acatgtgcca tgtttgcttcacgtatcatc ccatcaccta 33361 ggcactaagc ccagcatcca ctagccattc ttcctgatgctctccctgca cccatcccac 33421 ccgccaacag accccagtat gtgttgctct ccaccatgtacaatggctat gttcaaacag 33481 atgaatgata acaagtgttg gcaaggatgt ggagaaaagggagcccttct acactgttgg 33541 tgggaatgta aattagaacc accattattg aaaatagtatgaaggttcct caaaaatcta 33601 aaaatagaac taccataaaa tccaacaatc ctacttctaggtatacaccc aaaggaactg 33661 aaattggtac atcaaaaaga tatttgcact cccatattcatttcagcttt atttacaaca 33721 gccaagatat ggaagcaatc taagtgttca tcaagtgtgcataaagaaaa tgtgatatat 33781 atatatatat atatatatat atattatata tatatatatatatatataga ttatacaggc 33841 cgggcgcagt ggctcagatc tgtaatccca gcactttgggaggccgaggc gggccgatca 33901 caaggtcagg agatcgagac catcctggct aacatagtaaaaccccatct ctacttaaaa 33961 atacaaaaaa ttagtggggc gtggtagcgg gcgcctgtagtcccagctac tcaggaggct 34021 gaggcaggag aatggcatga acctgggagg cggagcttgcagtgagccga gatcacacca 34081 ctgcactcca gcctgggcta caaagcgaga ctccacctcaaaaaaaaaaa aaaaaaaaaa 34141 aaaaaaagat tacacagcct tataaaagaa agaaattctgtcattcacaa catcatggaa 34201 tggaactaca ggacattatg ctaagtgaga taagtcaggcacagaaagac aaatattgtc 34261 tttaataaat gatctcactt tatggggaat ctagaaaagtcgatctcaga aacagagagt 34321 agaaaggtgg gttaccagag gctgtagagg aggagggagagagaaatgga agatgtcgat 34381 caaacagtac aaaatttcag ttagattgga ggaataagttttagtgatct attgcactgc 34441 atggagaccc cagtaattaa taacgtatta tatgttttacaatttctaaa atagattttt 34501 gacattctca tcacaaaaag ataaattggt gaggtaatatagatatgtta attagcttta 34561 ctaaatcttt ctatgatgta cacattttaa aaatcacattatacccaaga aatagataca 34621 attaatattt gtcaattaag aataattgac aaataaattttaaagattaa taaaaaatac 34681 attaagttaa aacattcaac atataatagc agatatgaatgtacacagaa atgacaaaat 34741 cccagcactg tgggaggccg aggagggagg atcacgaggtcaagagatcg agaccatcct 34801 ggccaacatg gtgaaacccc atctctactc aaaatacaaaaattagctgg gcatggtggc 34861 atgcgcctgt agtccagcta ctcgggaggc tgaggcaggagaattgcttg aacttgggag 34921 gcggaggttg cagtgagctg agatcgtatc actgtactccagcctggcga cagagcgaga 34981 ctctgtctca aaaatgacaa agtttgagca acattaatttggactgtaaa ttcttaaaga 35041 atagaaaagt catgtttttc ttctaagaaa tattatgtttttacattgca agaaatatgt 35101 gacacagagt ttttcttaat ctctcttttt tagtatattctagacaggca agcacatgca 35161 atttattgag cacccagtgt gtgctaatgc accaaacggagctttacata cattatagct 35221 aatggtctca ataacttttt cattttattt tcccctttcattatcgccac ttataaatga 35281 aaaaactgag ctccagagcg gtaaggccta ttgcccaaaatattagatag gaaacaacag 35341 agctggaatt tgaaaccaag tttgcctaat gaaaaacatttatgttcttt tcactgtaca 35401 aagctgctgt aagtatcact ggtgcctact ccaggccagggagtaaatat cattcatcct 35461 tatcacatac aaagtataac aaaacaaaaa caagaagctctgaatactaa ataataataa 35521 taaatacttt ataaaagata aactattctc taagagtaaaagataataaa cctattgtca 35581 ttgtagttgg tcactgaact taactaattt cctaatggacgcagcagaat tgtttgttgt 35641 ttaagcaaat atcaaggcat accccccaat ccctgttacagggaataaaa aaagtcttct 35701 tcaaatgcct ttcaaaacta atgtgaatat taaaataaagtgctactgat ctctctacat 35761 tctagaaaga tctcagacta acagtgtcaa ttaaactattacaacaaata gccatgtaaa 35821 aactgctccc ctactagagc tacgctatca caaaattaaccccagcaatc tacagttcta 35881 gaaaaagagt tggtccctcc tgctgaaaaa gagaaaaccaaaccctacat attttgtgaa 35941 tccactgaaa ataatgacat aaacagcact gcacatccatcatgtattta tatcttaagt 36001 gatagctaca gtctatgtaa ctatcgcaga aatcttcttaaactgaacaa aagcattatc 36061 tatcatcctt tctctctaga aaaataccag tttgtttaatctacccataa cattagaaaa 36121 aatacttctt ttatcatggg caatccttat gtttcggaatttttttcgaa aacgtagaca 36181 gttcataatg ctctcagaca gaagttacca ctactaccccaaatattaag tcaaaatgta 36241 actgaaaaaa agaaatttat ctagtaatga ttaatttattgaataattaa aaattaggag 36301 tttttgataa ttaaatgttg ggaataatta aactttcactaaatactggc acaggaactg 36361 aacggatgac aacagagaca agttaagatt tgttttaatcctaagtttta tgctaaggta 36421 acatcaaggg ctctactttt cagtgaaaga aaaatttcctttaaaattgt ttccctcttc 36481 cattattact taaaagcaaa gaaagtattt tttctaacagtgattatagg attctcaatc 36541 tttcatatta actttaaggt actacatgta agctacataaatctttccca aatctacctt 36601 tattgagtag atgaaaaagc agatatcgaa ctcaaaacatctaaatgttg attttctata 36661 attatagaag atattttctt ttgaaattta aagtttgtatttaggcatat taacactttt 36721 ttaatctaag atttaatatg ttaatgtaga aaacaactgaggctgttttc tctctataaa 36781 actaacatgc cttttccaat taacttagtt tccttcttagcaaaagttta acatttatat 36841 ctgttgagtt acaYtgtagt tgcataatgt ggaaatatcttaagatttag ccacttctat 36901 gaagatccta tatttgttat aacacccctc ttgcccagaagaaaatacat caacaaaaag 36961 tcctactata tgttctgcat tgactattac aaagcagtagggggagcagc cagagaaagc 37021 tctgagacaa agcacccagc aaagcaattt aatttataggcaacagaaac atgctagaga 37081 ggtttgccaa aagccaaata cacaaaataa attccataaagaagtcatga ccacatttgt 37141 aaagctggcc taagtatatt tggaatgaag tatagaaaaatctgaagtct cttagaaagg 37201 gtctggattc taacttcaag acagactttc agcttgaaacacagtcattt ccagccatgc 37261 caaacctcaa actgccccca aaattagagc agaaacaaaatacattttta tagtgatatg 37321 tcttgggaat aactcaaaat gatttcgttt tctcctttgaacctcactga acttacaggg 37381 aaggcctggt gaaatgtgca ttattaggtc acaagaggataacgttacag ggttccatag 37441 cataatcctt gaaagccctg aggcccaggc cgctgtcctgctctcctctc tactgactcc 37501 catcctcaat atactttcca tgagagaaag gtaaccaaaaacatggtgaa actattctgc 37561 ttaaaaggtg ttttactgaa aaggcacatt gaccactaacccttattaca tttcacatgt 37621 tctaaaaatt agtaaggtct gcattttcag ttttagagtaaaacaggaaa agtacttagt 37681 tagctcttct gtctggcatt ctgtaactgg aatttaagaaaagcctaaac ttctaattag 37741 aacttcagaa tgttcatgtc tgtattatac cacaaaaactgtaaatataa tttaagcatt 37801 tttatgatac agaaatgtgc gctatagaca gttatctaagaattataaaa atccatcaaa 37861 cttgaagtgt ctggtataaa ttacaaccga atggtaaaaagacattttag ccacgtaggc 37921 aaccaatgaa gtttgaccta aaacccaaat aatagcaataacaacagata cagtgcctac 37981 tattgtcaga tactatatta gctagctctt ttgtatatattctttKgaac tcttacactg 38041 acttttcaag atagttatta tggtgcccat tgtatgctaaggaaactgaa gctaaaaaaa 38101 attgaagtca tttctctaat gtaactccat tagcaagaaacaaaaccata attcaacttc 38161 caggtttgcc tgcctccaaa gctttgagac ctcactaaaattaccctcaa ttgtagcaat 38221 gaggttccaa aggaaatctg tacaaatctc aggcagattaatgtgcttat aagggtaaat 38281 gccatagatt taatcactca aattaaatgc catctggaatctcttaccct acttggtaca 38341 agcatcctac atgtgttaca gccaacccta gccttggccaagaattcaat aattttYctg 38401 cttcaatgcc atcaacaagc aagaaacata tataataccaatacaggaaa tacagctagt 38461 tgctcctaga gcatcattta aaagctgcta attggctaagatgcctttta ttgagaagtc 38521 taagaaacag aaataatgtg ttaacccagt ttgttaaatgggtttgagga atcagtttcc 38581 tccttcctaa tacctaaatc catctccata ataagtattctctatcacca tttaaaaatc 38641 ctgatctctt actagtgttt ttattgtgct tttcaatctRttagagtaaa ttttttaaaa 38701 cctcaagaga gaacacagaa aactgtcata atttttcaaaggctaaagga gaataaactg 38761 aaaatatcag tttgaacacg tgtatgtaaa aagaatggatggtaataaaa ctaacaactg 38821 aaaagcaatg tttctgactc attcattcat actcaagaaatatcaaataa ctgagtggag 38881 atgatgcaag ctgtacacat ttccagttca gactgaaggtgtggcaacac tcacaggggc 38941 cttccgatca gctgctccaa ctcagtttac agcttttgtggatgtggctt attcatgccc 39001 ttgttatgaa gaagaagggt aactttacaa cctttagcaagagctttttt ttctttctgt 39061 gctaaccctt ttttgtctat aacagtcatg taagcttagcactgatttag aaataaataa 39121 gttaaagaag acacgaatgg cttggacatt aaaagtttaagcaacagctt aagctgagct 39181 aagtttaatg ttaaaaatta gtgtgacagc aataatttttcaattcagct ctgtcgtggg 39241 ctaggaaaaa atatcctaat ttttcagcca caagacataaacattgctag aatgactaca 39301 ttttgaaaaa attatcttct cactttacct agatttcattttaaccaaac agaccaacct 39361 ctcctattaa acctgtaaat taactcaaag tctaatctggggccttcata ataaagattt 39421 tttttttaaa tccctgtctt atctggtgcc tttaaaagaaaggaatcatt ttaggcaatt 39481 attatgacct aaaaaatttt taaatcttaa cgtatagattaattccttga cagctgaagt 39541 catttacaat acattattaa atttcacatt cagttttaaatccaaacata gatctcaaca 39601 ttaagaatat ggattttagg gaaagYaact agctgctcaaattcatttag ctgaaaaact 39661 actgtcgaat caccttgaaa aYatacacta atgcttgcacattgctaagK ggcttttgat 39721 tctgctcttc attttcttac acagMatcaa ccacacaatagaattaagaa ctcaggccca 39781 tggcaaagtg ctcccattta gcagttagga cactggacatattcagcata tgtctactgc 39841 tattgagaat caaattatga aaacaaacta tattttataaaactaagaat ttgttactca 39901 Ratgagaata aaggtgccta atgcYacagc agggggtaaaagtcccagta agagagcaat 39961 aattggtcat tctgatgagg agctgtgcca aatttagaaatgaggcacaa atgacaaaaa 40021 aattgatcat cagtgtggac atgggtaggt cagtgtgcatatgggtaggt tagtatctgc 40081 tggccgtgtc tacagagccc cttttattta tttgaagccaacttttcact tgacaatgct 40141 acctacaaaa gcactgttaa tagataagac aatcaagtggctgttatgcc gtggcctttt 40201 tgtaaaaact gcatgagaac aggtgggata aaaaccatagaaaggtagaa aaaaaaaagc 40261 atcactcatt taaagatatg tgcacataga tgaactgtaagataatataa ccttcttgac 40321 cttgtaaata ggtggccaaa aatacttaaa tatctKgacgaggcaactat tactaattgt 40381 ctcacaacat taYcagtcct tactggctga atccttcagccatctgcata cgacatgcac 40441 aggcaaatag ccactaaaaa catacacaat tccaattatctccttgcata actaaagaca 40501 aaaagaacag gaaatccaaa aaatacagca aaactgaaaacactatacct tgccttccgt 40561 gctattttaa ggctgtccca ggctgaaggt tacttctcagccctcaaaac tccattaact 40621 gccaagacca gcaccttagc acatatggca cagatgcctagttgaagcct ccttgtacta 40681 aatgacctca aaaaactggg ctttctttcc aagattaatggaaacaaaag taatgatcat 40741 aaggaacctt ctaactctgt taatgtttcc tgcggatatgtatggccaac tgaatatata 40801 aaggctcagt tcttgcgggg taccctgaca actagatgaattagggtgaa ggttccaagt 40861 tcgatgaact cttctgagtt tgagtgccct aggtggtgacattcaagtga tttcacagac 40921 atccaccaat tcttattttc aaccgggaat cccaagaacacctttaaaca tgcaatgccc 40981 atggacccta ctgggtttct gctgcaaggg ctcagtgttacttaggaacc ctgtgtcttg 41041 ggagatactt aaatttagca cgtcaaattg cttttggaaaatgtttatct ggcgtgacta 41101 ggggaggcaa agcaaggaaa caggaacctc cctctaaggagcagaatagc caacatgcag 41161 taagcctaaa aaacctctca atttatttcc cggggctatgggtaccagtg gctcgccggc 41221 atgggaatcR gcagcctggg ggcaccagtg acagcagaaccggggcttac cttgttggag 41281 gccatctcac tgacggagtg cctggtctgt agggtctctagctggtccag acaccagtcc 41341 agctcctcca gggtctcgct ggccagtttc tggtaggcctcctctgcgaa gagacaggga 41401 aagggggact cagttctcaa gcgcttcacg ggtccgctagcgagttcaaa gggggccatc 41461 ctgccatacc ccgccatgct cggatgcccg gtgccggcacatgagggctg ctccttcata 41521 ttgcccagcc cggcagtgca acgggggcgg aggctgtgctcgcggggcgg ccggcctcgg 41581 ggaggcacgg tctctccagc ttggcgtctc gggtcggcctccagggaggg cgtgcaaaga 41641 gcggcgagcc aactttcaca cagcgacgcg agcggaaaaaccactatgcg acttttcaac 41701 tgtgcggcgg gcccgagtcc cagtccagga gcYcggctctagcggatggc gagggggtgg 41761 cgagcgcgct tgggtgcccg ccccgcagag gagcgtctggtccacagccg gatctcctcg 41821 gtccccaacc cggagccgcg gccccacgcc cgccccgcctgccgagcctt ctgcacKgca 41881 cttgaagtga atgaatcagc cgcggccggg tgcgcggccaccacgtttcc tgttttcttt 41941 ctcaactcct cgggcggcag gcgcagcgcg gctccaacgccgcagctttc cgggaacact 42001 ccccctcacg cccctctcgg ccctcggcgc tgcctggctcctcctcaacc cccttacgaa 42061 aagattccga attgcaaaag cctgtccctg tccgtgccacccgccgcgtc cttcccctcg 42121 agctctgccc cgggggcccc gccacctcgc agtgccatccccccggcgca gccgggcgcc 42181 actcgctttg aagtgcagac gaggaggcgc gggcgcgagcgtgtgtgtct gtggggctgc 42241 tgtgggactg tctgtgctag gagagcggcg gaaaacccggcgtggagcgc ctgcagtccg 42301 ccgtccgggg tcgcgcccgc cgcggggctc aggagggaccggcacgcagg cgcgcacggc 42361 cgcgcacctg tctcgctaga accccgcgcg ccccgccgagccactgccct ccaggttgcc 42421 cagaccgctg gagcctctgc gactccacga ccggctgcttcctgcgaagt ttcacaaaca 42481 aggttattta ggaccctcaa ggtttctgag ggtttaagttttagggtttc tgggggttcg 42541 tttctaaagg cttaggattc acctacttca aggaggcagggttgtgggaa ttaagaccgc 42601 attctttttc tacccagccc agtccctact aagtaccagacttctaaatg ttggggcgga 42661 cagaaacaag caaacaaaat atacaatgtt gtggggtcggaacgctttat aattcacaaa 42721 tttgacaata ggctgcattt tcatatgccg aattgcctaagagttctctt aaaggaacag 42781 caatgtgaaa gaaccacatg gtaaggagaa tttacgcatgtcctgcatac taotcctttc 42841 ccctgtgaat tgcataagac ctagatgatc ttgggccaaggaagaaaatc tggaagggtg 42901 tttctaccca ccatgtgcag agtacactac aattttgagaaaacggaaag accatattca 42961 aaggcataca gaaaagcctg ttctgtttac aaaggctgacccagggcacc agggacactg 43021 tccctaagca gatcagttgt gggagaactt ttctacattagcatgcaata agcaaaccat 43081 tcatagtgag gagaagtcaa actcccaagg gtccatttgataaaactttg tcattgtcga 43141 gtggaaagaa gatactgaga aacttacact aagtggtgggatcccttgtc tttgtctttg 43201 aatctacagg tattggagaa gaaattctgc ccttgaaatttagactccat aaattaacat 43261 ggcacaacaa ataaagttca tggactgaat ttaggctcctgggatttttg agatagcttt 43321 ttatgtgaac tttgtaaaag ttagagtaac ttttaaagtagttgttacat gtgccacgtg 43381 gcttattgtt atcaagaata cttgaactat ttactgttcactttgagtac tctaggttaa 43441 aatttgcctc ttttttgttc tacttcctcc accccgcagtctatgtaaat atatacttta 43501 gatggacaaa caactgccta aaccttgcca ggtgaattaaagtttcagat tgaaKctttt 43561 ttttccccct taacaagctt aaagatatta ataattttaaatggtaaata taaaatacct 43621 gctctgtata gcttccccaa ttctaagaat gtaaaaaatattacatgctt gttataaaaa 43681 ttgaagcatt acacacaaat ataggaagtc cattgtaaccccacacccta aagatcacaa 43741 acaatacttt cgcctatggc tctaacacaa tcattttcattttactgaat aacttccata 43801 tgcaagtata gcatcaccta gttgccatgg tagtgcatctactatatggt ttggttttga 43861 tattttcctc tctctctcat tcccataata taggaatgtaggaaatttta agaggacatt 43921 gacttgtttt atgatcattt gagctgtcca tagttagcctatccagtgga ttaaagatgc 43981 ataaccattg tgtctgggat ttgttcagtg ccatcttttcaatcattggt tacaatgaag 44041 ctgggagtcc cctctgaggt tcggcKttct gtggctcctctcccctgcac tccaactttc 44101 tcaaagcctg agcctcaagt gatttcccag tagYtaagtaaactgccccc ttcctccatc 44161 ttcaccaagt tcccaaagct Kttcagactc aggcttcaaatctaacagga tgtggctcca 44221 cacattatat ttcagttgtt attttttaat gtggaaaataattcaggaaa gttttggcta 44281 atgcttcaag acatcatgca ctttatacac aggatttgttcttaagaagg tagtatagtg 44341 acaggaggaa caatttactt cagaatatcc attttatgtccaagccatgc accagccact 44401 tttgacagac aggatttgag tatgatgata aaatggggtaagcatatatt ttaagttcta 44461 acatctgtat aaataagaaa aatgaaaaat cactggaacacatctacatg ctagtgagaa 44521 aataattact aacagagtaa agtttgctct gtgaaactgttgctcaaata aagaattatt 44581 cttgatatta gcatagagct gagaatattg cttttttccaaattcctagg tgagtaggca 44641 atatagctag ccacgatttt tcaggatcat ttgtaaagcttcacttttag gaattttaac 44701 cccaagtgtt ctaagacatc aagtaactga ccttttcatcccgccgaaca aacctcaaag 44761 tggagttgcc aactatccca caggaaattg agtcaccacaccaagcaaca cacctaaatg 44821 tcgacagcta gcaggacaaa aacagtgagc cagtttaattttggctttta ccaagaacac 448B1 atactgcatt cattaataaa atacttcccc tcaacccctgccagagtagt atacactgtg 44941 gctctcctta aaaatacatg aaaactcttt aactgcaccaaatcttgcta agtcaaggaa 45001 ctaaacagag aaaaacaccc aMgcaaaaac tattggctaaactccctaaa ggctgggaga 45061 gggctatgga acagcaactg agaaataaat tgagccttgaaggcccctgc tacaacagca 45121 tcagttttct tcaaggcacc tttgaactat gtaacacccgcattctgctg aggctcttct 45181 gtgaacaaac agtcctcaat tcttgdgggc cagcacttggcttctgtcac agtgatatgc 45241 ttagaggttc ccctgacaca tggcctgaaa ctgtgccaggggctccagga gaagatgctg 45301 aagaagcagc ttccttttga aaaaggaaaa aagtaatcacctcagagcca cagcagttag 45361 ctagaggttg aggtcatttt ttgtgaaatg ttcagtccattactcttacc cacaactcac 45421 agtgatgttg accattgagg taaaaggatc cactgtacgggatgtgtaac tatgtggaca 45481 ttagtttctt tgccaacact tgaaggtctg ctaaaggactgtatcggctt gtcaeaattc 45541 ctagatagac ctattggtat acatgaagag aacaagtccaaaaaatacca cttattttgg 45601 acactatttg tatgccctta atttaaaaaa aaaatacaaattataacagc tgaggtcggg 45661 cacagtggct cacgcctgta atcccagcac tttgggaggccaaggcgggc agatcatgaa 45721 gtcaagagat caagaccatc ctggctaaca cagtgaaaccttggctctac taaaaataca 45781 aaaeactagc cgtgcatggt ggcgggcgcc tgtagtcccagctactcggg aggctgaggc 45841 aggagaatgg cgtgaacccg gcaggcagag gttgcagtaagctgagattg ggccactgca 45901 ctccaacctg gacaacagca gaagactccg actcaaaaaaacaaaaaaaa ttataacaac 45961 tgatccagat tttttttatt attacacttt aagttctagggtacatgtgc acaatgtgca 46021 ggttagtttc atatgtatac atgtgccatg ttggtgtgctgcacccatta actcctcatt 46081 tacattaggt atatctccta atgctatccc tcccccttccccccacccca cagcaggccc 46141 cggtgtgtga tgttcccctt cctatgtcca tgtgttcgcattgttcaatt cccacctatg 46201 agtgagaaca tgcggtattt ggttttttgt ccttgtgatagtttgctgag aatgatggtt 46261 tccaatttca tccatgttcc tacaaaggac atgaactcatcctttttcat ggctgcatag 46321 tattccatgg tgtatatgtg ccacattttc ttaatccagtctatcattga tggacatttg 46381 ggttggttcc aagtctttgc tattgtgaat agtgccacaataaacatacg tgtgcatgtg 46441 tctttatagc agcatgattt ataatccttt gggtatatacccagtaatgg gatggctggg 46501 tcaaatggta tttctagttc tagacccttg aggaatcgccacactgtctt ccacaatggt 46561 tgaactagtt tacagtccca ccaacagtgt aaaagtgttcctatttcttc acatcctctc 46621 cagaacctgt tgtttcctga ctttttaatg attgccattctaactggtgt gagatggtat 46681 ctcattgtgg ttttgatttg catttctctg atggccagtcatgatgagca ctgatccaga 46741 ttttttaaaa ataaaagaac tacaacaaaa agctcctaagagcaaatact taaatctccc 46801 taaccagttc cagcttctct tctcttaatt cttacaaaatttcccctttg ttctaMggaa 46861 ggaattgata ctgtctacag tagatagaac tggaaaaaatgatttctgtt tcaccgtgta 46921 ttgctttgag ctagtcattc ctttagtttg ctacagtaaaagatttgtgt aggttaggtc 46981 taacttttgc tgacttaatt ttttttcctc tatacattttatctgatggg aaatacatga 47041 aggctttgga ttctctttca atgtgtacat gtaataggtttaagcagagt ttgctagtgg 47101 caagctgctc tcatctccaa gtgtatttct ggcatcaaattattgttttg aagtatttta 47161 aaattgtata aaatatataa aactttattt agtacttcataaagcttcca aaggaaagat 47221 gtgtgcaatt ttgtgacggt tttctttttc ttttatcttctcactaacta caattgtttt 47281 tccgaatgtt gcctatgcct ctagtagaat ttttatatgaaataaacatg aggttggctt 47341 tcattgactt ttagtcccac cagtaactca acatttatttagcactgact atgtgccatg 47401 cactttatta gaggctgcag aaacaagatg atgaaaagagtgtgtccctt acccagagtc 47461 cagcagagga ggtaatttca gcaaacaact gtagagtgcactctcacaga aggaagcatg 47521 tgggctatgg gacagtaaaa gaggactatt taaatcagacagggacagag gtggagtggg 47581 aaaaggctga gtcctcaaga atgaaataaa agagagtgggaaggattttt aagagaaaag 47641 atgagcaagc acacagacct atgatgaaca agatgtctgtgtcggccagg cgtggtggct 47701 cacgcctgta atcccagcac tttgggaggc cgaggtgggtggatcacctg aggtcgggag 47761 ttcaagacca gcctgatcca catggagaaa ccctgtctctactaaaaata caaaattagt 47821 cgggcttggt ggcacatgcc tatattccca gctacttgggaggctgaggc aggagaatcg 47881 cttgaacctg ggagatagag gttgcagtga gcagaggttgcgccattgca ctccagcctg 47941 ggcaacaaga gcataaaaac tctgtctcaa aaaaaaaaaaaaaaaaaaaa aagatgtgtg 48001 tgtcaaaaaa gaaataacca tgagtagggc cttgcttaggggaacagatt ctggagaaat 48061 ctgagaaatc ctcagccact ggccatctca aagactggccatgtcaggca tctgcaggta 48121 ggactatatc ttcaagagta ggagtcacag agtgactcagagtactgtgt gacatgggat 48181 ctgattttat tttatatgga aaattctagt gctatgcaattaatggaatt Kaagaaagta 48241 aagttgaagt cagtgcaacc attatctttg accatattaagaataaaatt aaataactta 48301 tagaccattt gcttctctga cgattattct tatctaagacaatctctgta ttaaaatcct 48361 acgcagacat atgttcccac aatatgtcag gtatgaacagaaggactgcc caatgtgact 48421 tgaaagggag tggacttctt gctcttaaaa actacaaccttgtagtcact aacttattaa 48481 tcatactgta ataagatgga gaaaaaaaca atggaattcaccagaatttc tttcacgaga 48541 gtctgcctgc attaaaatgt tcgataaagc tatttatgacagaaggtgag actttgaaga 48601 gctttggttt gggtggaact aatctgtgcc tcatctacacaggaaagggg aggaatatgc 48661 cagttgctag agagggaata tgtatgtgtg tgcatgtgtgtaagcgcatg agagagcgag 48721 agaatgtgtg tttgtgcaca tgcatgtgtg tgtgtgtaagagaaaggagt cagcttggtc 48781 tctgtgtgtg catgtatgta agggcatgaa agagtgagagagagagagag aatgtgtgtg 48841 tgtgtgtgtg tgtgtatgtg tgtgtaagag aaaggagtcagcttggtcta aagggtaact 48901 ccaatttgta catttttcct ttctcccaat catcaataaggttattgaag atgtgtgtgt 48961 gtgtgtgtgt gtgtgtgcat ataagagaaa ggagtcagcttggtctaaag ggtaactcca 49021 acccgtgcat tttccctttc tcccaatcgt caataaggttactgaaggta caaaatcccg 49081 acttgttttg ttttgtttta ttcattgaag ggactgagcttgaaggccag attggcccaa 49141 gcagaaatgt ctctctgagc tgacataaga gaagagtcaaagaaagagga gaaagctttt 49201 ataagaataa ggaggccttt tgtccaaaaa attggagtttgagctaagtt taattaggca 49261 tctaggttgg gggcttcatg tgacatgtgt gcaactgtgagtgatattat tctttgaaag 49321 aaactggaag agtggagaaa gattccaatc tctattccacttcatacaag caattccagg 49381 caattcagct actaatcttt ctagggcctt atttcacctatagattggag ataataacaa 49441 gagccttgta agttatttta gttcaagtga gaggttgtgagttaaataat cttgcattga 49501 ttgcctaata gattcttttc ccttcctcct ccattgtatcttcactttct cctggactca 49561 agtctctaac ttaaatgcaa acattctatt aattttagtgagcctatcaa ctaataccta 49621 ggtataacct cggggtccag aaacttaata gWtacctggaacagttttta gggtagttag 49681 aaagaaaaaa gaatttcaag gaatgaacag ggattcattctgaagaagtt cacttagaga 49741 acacaggcca ttcaggaagc tacaaacagg tagggatataaagtttagat cagacagtgg 49801 acaaagataa agatggagaa ctagtcttgg aggacttgtatactattcta agacaatatt 49861 ttcttctaaa gaggccatta aaaatcttct aagtgaggaagtcacatggt cagatttgct 49921 tttcaaaaag attactctgg gaatagtata aaggcagatcagaaaagtaa agaccagtta 49981 agaggttggt gaccagataa ggagaaaatc ctaagcagtccctgaagggg aaggagaagc 50041 tgcagaaagt taggaggaaa aactctcagg agcaaagaaaaaggaaatgt caaaggaaaa 50101 tattgttatg gactgaatgt ttgtgtctct ccaatattcatatgctgaaa tctaatcccc 50161 ggtgtcacgg tatttggagg gaaagccctt cggaagtaattaggtcatga gaatggagcc 50221 ctcatgaatt agattcgtgc cattataaga agaggccagaggttgctctc tttctaccat 50281 gtggatacat gctatgtggg cagtctgcaa ccctgaagatgccctcacca gaacctgact 50341 atgctgacac cctgatctca gacttccagc tcccagaactgtgagaaata aaWgtttgtt 50401 gtttaagcca tcagtttatg gtattttgtt atagcagcttgagctaagac aaatatcaag 50461 gctttgccat tgatattcag tacagaccgt atatacccacaggtttgcgc taagataatt 50521 ttcattccaa acacatattt cagcaacaca agactaaagagggtgttaaa gaatgctact 50581 gaggaagctt ttgtctggga agttgtttga acaaagttacaggacaattt ttgcaaacag 50641 gtttaaaaaa agttacaatt atgtatgcta tttacgtattcttgttgtaa ttttaggaat 50701 caattttatt tccctggggg gactgtagga gagcatctgtgtttccatca tatactatca 50761 atcttaggat ataacagtct tttgttcttc acctttgtcaattactgcat acagtattcc 50821 ttagagaaaa caaggagttt gaacagatta tctttagcacctacccagct ctaacattca 50881 tttattatct gatttctgaa attgtactat ctgggttaggtgttggtttt aaggttgttc 50941 attatagtta gcctggatgg ggtcagctat gtttgtagagggaattccta aactgaccag 51001 ttacttgtca aggatcccat ccaataccat gatcctggaattctgtgagt ttagctcaac 51061 tgttcttcag gggtacatgt tttctatttg aaattgaggtatYctcagaa ataaagctat 51121 taataaatag aaagttctgg agtatattta cactcactctgcttatctcc actcctaccc 51181 ctaccatatt cccatccaat actttgccag ttgacttaccaatttcttct taatttctgt 51241 aactgtcacc ttcagtcaaa ttctcatctg tgtatttctagattcatata atggtctact 51301 actggatctc cttggattca ctttttctca acacagtcctatttataaag tcagaataat 51361 cttccttaaa ccctggtttt attgtttcat tcttttaagaacctgctgca caactctctc 51421 ctacttattg tattaatgtc acacttctgt tcacataggcctttttcggc tgcttttcaa 51481 tacctttccc ttttatttac atctaagact atcccatccaagactgccca tcttagccaa 51541 tctttccacc tggaaataat ttgctggctt ctgcctgtatgacttttcta atgtcatttt 51601 tatatttgtg tctaggcctt tctataactg tctagaaaattcctgacatt tacaggcact 51661 cagcaaatat ttgtgagtga ataatcagta agtatgcttgttttctatca gataagaata 51721 tttatttcca aaYgagattc aaacagtaat actggcctgggtgtggcttc ctacatgtaa 51781 tcaccctcgt ctatctcttg ttctcagtct tttgctttcaaactctttct ctttttttaa 51841 aagagtatta ggaactaaca ttgccaaaat ttggtaatggtcaagcagat catggtgtct 51901 tactgaagtg aaatattaac attaaaataa aaatgacaagtattatggta tatcattcat 51961 atgtaatgat ccttacaaaa tagagtaaaa ttttaaaagaattcaccaat acaccagtat 52021 gatgtttggg agaaacagat gaatgcacaa aggtgcatctgatgcaaaca cccccagaaa 52081 aatgagcata agttcatatt atcaatattt ttcactctaaaagtgttcac tgacttccct 52141 gaagtcacca aggactccaa gttaagaata ctttcctgagtgaagaaaag aaagcaatta 52201 ttttaatcaa cactgcccca gaatctttta ttcggaatcgctatctctgc tttcttcttc 52261 ttcttttttt taactttctc ataaatctct gtttagcatttatatctctc tcactatgag 52321 atacctaagg gttaagaagt aatgccaaag agacatttgtccagttgaag actgggatta 52381 agaaaaaaca cacagccggg catggtggct catgtctgtaatcccagcct tttgggaacc 52441 caaggcaggt ggatcacctg aggtcaagag tttgagaccagcctggccaa catgatgaac 52501 cccatctcta ctaaagaaaa tacaaaacat tagccacgcatggtggcggg tgcctgtaat 52561 cccagctact tgggaggctg aggcaggaga atcacttgaacccgggaggc ggaggttaca 52621 gtgagccgag attgtgccac tgcactccag cctgggcaataagagcgaaa ctctgtccca 52681 aaaaaagaga aaaagaaaag aaaaaacaca caaaagatttcccgaaaaga ctaggccctt 52741 ttaaacaggc tatgaacaga gtctcagaaa ggtaatttcttatgtacaat ttttaatctc 52801 attacttgca ttacaagcaa cacatatgag tgataaactattttggccta ctggtaaacc 52861 tacatttgtt aactgtgtgt gcattataga gaacaaatttcagaactcaa gagtatatcg 52921 gttatatttg gcttatccag ttgtatatga actggctccatacacgaata agataaatta 52981 gttttataat tttacaagaa tatctatgga gatttagatttggctaagaa agttattata 53041 cacacagaag aatccacaac ctataagagc tcttcatgggaaatagtgtg atagatcaga 53101 aagagcatgg acttttgagt cactcagaac ttgaattccagccttgccat tgttctagtt 53161 gtattatctg aaataagcca tttaaaagac ctgagcacattgtatacatt gagaattaca 53221 gggttattat gagaaatgca taagaaatgt aaagtaccaagggctgtatc tgacatatag 53281 aaggtattca atcaatagta ggtatactga accactttgcagagttgttt aaataagtta 53341 attttaaata aggtagttaa tatttgagag tttccttccttttccacaga ttctcaatcc 53401 taaaacagtt actatttttc catcatcacc atcaaccctcaaatattaac cagttctgag 53461 ccaaatgtga gtgaacaacc tatgatttag aatagcttttagaccaggca cagtggctta 53521 cgcctgtaat cccagcactc tgggaggctg atgcgagcagatcacttgag gccaggagtt 53581 caagaccagc ctagacaaca tggcaaaacc gcatctctactaaaaataca aaaagtagcc 53641 aggtgtgtgt ggcacatgtc tgtagttcca gcttcttgggaggctgaggc aggagaatca 53701 attgaaccct ggaggtggag gagatggtgc cactgcactccagcctgggc aacagagtga 53761 gactctgtct caaaaacaaa caaacaacaa aatagaatagcttttacaag accacaccaa 53821 atcatgaaaa gtcaactatg taggaagaat atgtatttgtcattggttaa tgacagagca 53881 tcacttacac tgaggttact gggaagatca tttcaaacatttcccaaaga atgttccaat 53941 caatattgtc cttataagat tctccttact aatgacgttttgtggtcaaa caagcttggg 54001 aaatattgta tatgtaggct ttttacagtg atttctaatggacacgaacc taccaaaagc 54061 tagaaggtcc acagcaaaat gagatattta attttattaacaagcattat ctaaacctgt 54121 ttgattttag aatgcatttt tcacacaaca actatcacctcttgaagaat acctggaata 54181 ggttttccta tacctatacc tatatgtcag gtaactccaaaacattaaca aatacatgtg 54241 tttagtaggt tcccatatgg ttgaaatctc cactaacgacattttatttc tcttcctcct 54301 gactttgtat gctatcgaga aatggtcaga gagtaatcttcctccctccc tttcttcttt 54361 tctttctctc tttttccccc tgaaagagaa tagtcaaaaaatccatacat atttattgag 54421 cctctctgat gtgatagttg ctgtgaaata caagaacatgaaagattcag cagcctgggc 54481 ccaggaagga accataataa cctgctgaga aaatcagacacatgatcggc tgctgcctag 54541 agacagtgta aggtagcata taaatcattc agcctgggagtgctactgta gttcgagcag 54601 ggagagggag gtatggacta tagctcagtg tgctgggtttcaggaagaag gtgaacttgg 54661 atgtaggtct tcaaggaaga ctgtggtttt aatggctgtgggaggaggga gtccgctggc 54721 attgttttca ggYgtaggaa atgtctgcag cctgaataaaggttgtatgt gtggacagta 54781 aatgggctgg gtggagtaga cacattccag ggaggagaagggatatagag ttgaacactt 54841 aaatgtgtgg ccttgagcta gtggatattg tgggaattggataagtgcac tggtagccta 54901 agaggataga atggagaaga gagaaggatg gagactaaggagctattgtg gtagtccaga 54961 tgcagtggaa agcaaagagt tgtggaggta tccactttagtggtctacga tgacagttac 55021 aagcaataga aagagtatga cttgggaaca gtggttcaaatgctggctct gcaacttagc 55081 aaccatgggg caaatttggg caaattaact tctctgtacctcagttttct tttttgtaaa 55141 atagggctaa aaatacattc cctagaaggt ggcctccaggccattttccc acactttatt 55201 tgaatgttaa aactaacttt aatctaaaaa tcatatattcatatctctat cttttttggc 55261 ggttcagttc aacttaaatg tgttatttgt taaaaacttgtaattatcat ctattacatg 55321 taaaatataa gagctgcttt taatagtaat tcatctcctccctatctttt tttaaaacat 55381 ggtcaaaatt aacaaaacaa tcttgtattt ttgatactagtagtggtatc actactacta 55441 gaggtacaag gactcttgta cctctctgaa aagtagcataattagcaaca gtattgatgg 55501 tatctgctat tttagattat ttaaactgta gtcactaaggtaagcaaggg gaagaaggca 55561 tgtcatttat gtgtttttca tccttaagat tagcgtttggaagcatatat tattccacag 55621 caatctggat aggtcttgcc atgccctgat cttgtaagcccaaattttat tttgaatact 55681 ccagctttgc acttaaatga cagctccttg ataaaagaaaaacaattgcc taacaatttt 55741 cttccaattg tctgaaaaca taatgggtgt acatacacacacacacacac acacacacac 55801 acacacacac acacacacaa tctctagggc atatttactagatagtatta tgtaatgtgt 55861 agacattttt aattatgata attaagacaa aaatatgtgtagcaagtttt tgatttgaga 55921 caaatttgag atttactaaa attattatat gtgtgtccttctgaagactt catattttca 55981 ttatatatct tcattcttga agtataactc ttaaattgttgaattaagtt ttatgaatta 56041 agttgttttg ttttttgttt ttttttgttg ttgttttttttttttggcca ggcacggtcg 56101 ctcatgcctg taatcccagc actttgggaa gtcgaggtgggcagatgact tgaggccagg 56161 aatttgagac cagcctggcc atcaaagcaa aaccccatctctactaaatc taatcttgtg 56221 cctcaagcga ggcacaagac tcacttgaac ctgggaggcagaggttgcag tgagctcaga 56281 tcgtgccact gcactccagc ctcagggaca gagtgagattctgtctcaaa agaaaaaaat 56341 ttttttccat ttttaccaat accactattc tcaattcatccactatttca ctaggaatta 56401 ctaattcagt ttacataatt tattcaatga ataaattattcaatgaagaa gcttctgggt 56461 atttaggtgc tacatgctaa tttcaattgg catattttgtttttcaaatt tgttcttggt 56521 gacccaataa ttatacctgt ccaccttgaa agtgtctttcttcatcacct tcaaggccac 56581 cgctaacaag acctttgaaa agaagtaaac agtagagaatttcaacttct gtgatgacca 56641 ccgtcttctt ctgaatcccc agactccact catacaaggttttggggaag tttaaatgaa 56701 atgaagcagt ggcttacaaa gtttattgac agtaaactacagtaaaattt atattttata 56761 ctataacctg gtagatttgt ttaaagtaag aggtgctcacttttgaaggc atgtggatgc 56821 tggctttcag taatataaaa aaaatgtaca aaaaggttttatacctatat ttatttttaa 56881 aaggtagagg agagcagtgg actcctattt aaagaaactcttgtgtttta taaataatag 56941 tcatggcaaa atttgtttac agtctttaac aaaccatgcatttcagtata aattaaacca 57001 ataaaaagaa ttatatatat aaaataaaaa ggaaatgtatatataggaac agtatatacc 57061 atacacataa gagtaaatat atattcgagc caatacagagaattgaaaca taatttaaca 57121 tgaaataaca tttatcctta ctatgtatga ttcactctgatgttttctat ttWatttaat 57181 tttaaagtat taattgtgac cccttaaatt gattttgaaatctaccaatt ggtttgaaaa 57241 cactgaggta agaaattacc ttgcttaata aatgttagttttgtttccct cttccctctt 57301 cctccctttt tataattaaa aaactctccc tctacttctctacttcacgt gagagtagaa 57361 cacataaaaa gatttcagtt aactactgtg catattttttgaagtaattt ctccataatg 57421 tgtgagtttt ctcagggtac tacaagttta ccaacagaacacataaagca gcggccactc 57481 actgaaatgc ctataggaga cagatagacc atatgaatgaatgaatgaca tggttgctgg 57541 gcatcctggt tgcagtggct agaactgtgg agggtaggagcagaccacca tatatcctag 57601 gcatttcatt ggcaaatgaa caggagaagg acacctttccgtttggccct cattttccta 57661 gatctttcct cccttcctct gtcaatccct cctacatagcctatgttccc aaagcacccc 57721 ttgtattata ataataatta ttatgatatc tgtccttaatcaagaactta tactggattt 57781 ttaatgcctt taggtcttcc ccaaacttca gttcatctattcgaagtccc tataaaatct 57841 acctccttag acttgtctgc ccccattatt cccctgtggttgacctcacc tccatctcta 57901 tcttttgtca gcaagtttcc tcagagttcc ctctctgcatacttgttcat gctgggttat 57961 ttgtttttgc cacttgatca tctagacctt tttccctctttccctccttc tattcaattt 58021 ctgcctttca ctgctgtaca aaggaggggc agggaggccctccagggtgg agggaatcag 58081 tcttgagata acegagagat tcagggtgat gagtcccaaagttgaagaga atccacatac 58141 ttcttctgtt tttgttttac ctagcaagga gccttttctatagacaaagt tRggaaacca 58201 gaggttatga cgtgattcat ctttgcctac ttaaactttagcttgattca tcacttctca 58261 acaaatactt gttgaacccc accagtaata cgagaagcttggtattctat gaggtgctca 58321 acttccaaag cttagttcag aagaattctg gcctcaaggcgtctaccatc tagaagcaaa 58381 caactaacaa atgaacaaat gcagtgattt caaaagaaattaaggtgtgc tgtggtagag 58441 gcatgcacag gtcagaaaag ttcagaggtt ccagacaggcttcctgggga ggatatccat 58501 gccccScctc cacctggtag ttcatgatca gagtttgcaagcttggctgg gatagaggga 58561 atagcgtgtg aaccacagaa ggagtggatc tggtggctcagacacggcaa gaaccagctc 58621 atggcactag gctgcaggca caccatgcac aaaacagtcttttctttccc cagttctcca 58681 aattctctaa agtggaagcc taggggtgag ggtaagaaggaggtaggaag ctacaaccac 58741 tggagcaaag gacgaaagaa ggggcttgag tgtctaaaagtgcagaaact caaccgactg 58801 gacgctgaga caatgtttaa aaaggagttt ttttgtttgtttgttttctt gtgttttttt 58861 ttactttaag ttctgggata catgtgcaga acgtgcaggtttattacata ggtatacatg 58921 tgctatggtg gtttactgca cccatcagcc caaactaggttttaagaccc gcatgcattc 58981 ggtatttgta ctaatgttct tcctcccctt gctcccccaacccccaccag gccccagtgt 59041 gtgatgttcc cctccctgtg tccatgtgtt ctcattgttcaactcccact tatgagtgag 59101 aacatgcagt gtttcgtttt ctgttcctgt gttagtttgctgagaatgat ggcttccagc 59161 ttcattcacg tccctgcaaa ggacataaac tcattcttttttatggctgc atagtactcc 59221 atggtgtata tgtgccacat taaaaatgac tttttaaagtggcaactgtg ggaggcaaag 59281 gagaaaaaac aggagctgcc agataaggga gtgaagagaacccagcactt tgagaattcc 59341 gttacatttt gcagcttgaa ttcttcactt tttgctcccattatgtgcct ctttctcctc 59401 tccatgaact gggccctgtg gacccaggat cctgctgtctttgcagggca aagaatggtt 59461 agaaatgaat tcattaaggt gagaaaccat tgctggcataattctccttc acggaaacct 59521 ggcacttaaa tttaacctgt ttggttagat ccagctctgcctcccccatt tYtttctgca 59581 gaaacaaggc tggcagatcc ctgttcctct taatccttgaatggaccaaa tgctgggtta 59641 tgctggggca gtgagggacc ttacaaacac tgaaatttaagagaacagtt tttggagtaa 59701 ggcagtcctt gtttccaaat ctgagctttg ccactgaYtgactagcttgg caccttataa 59761 aagtgaatta acctagcttg ttttctaatt tataaaatggagaaagcagt acctatatca 59821 cagtatgatt ctgaaaatta tttaaatagg actatgtatgtaaagcagtg cctgaaaaat 59881 agcaagtggt cctaaagtca ctatgatgat gaattttatatgcaggcacc tagagcatgt 59941 taggattttt gggatctttc ccagctcaca ctgaagcttcactcttctcc aacagtctgt 60001 tatccaatgc tggataactg agatagtctt cccagcatttatcaatgtgg ttaggaacag 60061 taatcctgga aagcattctc aaattattta tctaattaaacaagcaaacc tttttagccc 60121 agacatccta aatctctgta gcttgtcttc gctgatttggtcagaatagc tcagatggta 60181 ggaagggctt tctgggtaga aaaaaataaa ttagcagtctggaaaacaaa agatctctgc 60241 ttaaaagagt ctctaaaaca gcctgatcag cctgtgtattttgccatatt atctagcatt 60301 agtaggggac tgggaaaaat ccagggctcc ttgtagagatagagactggc aaatattatg 60361 tttgatgcat gagaaccaaa tcagaagtta actctttaagttattaaatc actaaacagt 60421 ctatcacaat tatgtgttta ttactagact gaatttctctggggctggaa ccataatgaa 60481 ttcatttttt gatctctggt gctaaacact ggctgtcatatactaggtat ccaataaatg 60541 tttgttgaat ggaactgaaa aagcatgaaa cacttcccctgttaaggctt cttttctata 60601 atatttccat agtttctaat gcatctgaga atcattaactggactgttag aggaatatga 60661 atatggttga gggagctaca aacttcatgc tctgagatgtcacatgatta gtgtaacttt 60721 tgttccaggg gagagaaatc cctaagagag tgactgttctccatgcccac ccacccacag 60781 tgtcctatag agcctttcat tctccagatg cctataatgactatcacatt gatttgttta 60841 catatctatt gccttgtctt ccttataggt acagagtccatacatcctaa ttggcctgga 60901 acttccaagg tctatgcctg ttataccagt gtaattatgaatagcattgc ctttcattct 60961 aaaaagtgtt ctggtttgaa tgctaaatca tctagacaccctacttatga gagggttcac 61021 tgtacatcgt ttagtccccc aactcccctc cccttggaaatctgggtact accctccacc 61081 gggggctttc cactatggag gcagctctct caatcacagcattcaggagg tgtaagtcca 61141 ctcccaacag aacagacacc tgtctacaaa gagactcttggtggaagaaa aaaaaaaatc 61201 acttccgtaa gtttaagtct tccttttaaa cttgggtctcttttgcacat cagaaacaaa 61261 gaatctctaa taattacatc cctaaatgta ctgaagtctagaagacatgc ccatttgaaa 61321 actctaatct agtaattaaa gcttctcagg ttaaactgcaccttaataat gtagcttatt 61381 ttgtcaagat cagttaaggc cctgtaatgt gatttggaataaatggacag acagaaatta 61441 aaatgtgctg gacaaggtaa tcagaaatct cacttcatttagtcaataac aatggcaatt 61501 cagcgggttc cttttactac tgggatgtaa ttgtgcttggctttaatttc ttgtaatgat 61561 ggaacaaatt aaattacttc atacttttag gaacatttggctacatcatg ttttggYgct 61621 tccataaacc ccactggaaa ttaaatcagc tctgtggttgctcattctcc ctctttagtg 61681 ccatgtctgc cttctgtcag caaaatggta atattgtctaccttaatcca ccagatgcag 61741 ttgagttaaa agaaattacc tctcccacct ctttcctttcaaatctccct atttgtagct 61801 atcaaatttt ggcatgcctg ctggaacaca gtcttgttagaacaagcacg gtaattctct 61861 cgtgagactt taggactctc tctacgacaa ggtcagaatgtacctgtgat tcaaatttag 61921 atgaggatat gagttggtaa gaacatgaag agcatcagacagttttcagg acacccacta 61981 cttttatttt gtgctatcag tttttccttt cccaaattgcaggtggagat gcaatcttta 62041 acgctggaaa tctcttgcta tatgccatta aaattaattagtctagcaca ttttatcttt 62101 aggcccttac ctagggcagg taaaatattc tagcacaaaataacacaact ttcctgaaca 62161 ccatggctac taagttcata tggtaaagat tatactatacattaccttgc aaatagagtg 62221 ttgcagactg atattaaata tttaaaatgt atgcaggattattaataggc aacattgtgg 62281 cagagactgc taaatgtccc ctaatattga tgcttttcttcatcacttgt tttcaaatac 62341 ctgttttcat gtaagcatgt gactgcccaa aatagagaaaattcacagcc tccttggttg 62401 ccaggtgttg ccatataact aagttttggt gaatgagttggtagtagaag cgttatgtag 62461 atattatatg atgtgctaga aacttccctt aagagaaatttgtcatctat ctttttatct 62521 gcctgtattt attgttttct ctttcctact ggctagaatgtgaagttgat ggctggagtc 62581 ggagcagcca tattggatgg tgagttgaat ttagcaacagaggctacacc tcacagtgca 62641 ataagatgga aagagcacgt gtccttgaag actgtactggatagaggtgc catattaacc 62701 tcggactgcc aactttcaga ttttacatga aggagaaatatctgtcttcc ttaagccact 62761 tctattttaa atctctctat ttactgctga ataaaatctcaactaacgca aacaccaata 62821 acaaaaaatt cctattcact catgatatag gattataccttagtcttttt tgtgtcatat 62881 atctgttcat tcaccatttt tgctttgatg ttcaataaacatctcaaatt taacatatct 62941 ataaaaattt tttaaatttt acgttcaggg gtatatgtgcaggttagtca tataggtaaa 63001 ttgcatgtca tggggtgctg gtgtacagat tatttcacaccgaggtaata agcatagtac 63061 ccctacagct tagtcttaag aagaagtatg ttcacataggccaggctgag acttggatgt 63121 tggatgactt tcttccagct tactctgatt tgaatatattgtgtggtagt agatggctaa 63181 actgcttgct ccaaaactat ctgacaatgc tttttagaaagtctgccaaa caaaactaca 63241 agaatcaccc tggagcttcc cttaggcttc agatatttgtcttccctgcc ctacttggaa 63301 atcattccag cctggaagtc attgtgagga accaaaaaatggtctcccta ccatgaagaa 63361 taacactgct tccattgttg ggaggctctc cttcctacaggcactgtccc tcactcatta 63421 gacacttgaa taataaggat gttcatatat ttattggtgttggtatcagt tagacagttg 63481 aatgatacaa atgttcatat atttcttctt attcaatagtctcacaagaa cactattctt 63541 gtaaagtcat taaaaactgg atggtctttt cataattatttccatgattt gtcatctaaa 63601 gccagtgttc tctcctcctg tcaatctgat agctttaaagctaagttctg ggtgggtcca 63661 agtcatgcct gaaaggttta tcaaatgaca tattgcagtcatatggtcgg agcacaaaca 63721 cctctgaatg tacaatattt taaagcaaaa cagtttatgtgtaagctcta tgaggttacg 63781 gaccatgact atcttattta ttaccaaatc cccagctcctagaacagtgc ccctcaatat 63841 aaatctgtca aatgaataaa cgaagagttt gcctgcagtagtaaacttgt tatttgcaca 63901 cactcgaggc ccacctctat gtgtacattg tcagtgagtcagcctcgtgg atgactctgc 63961 aggactgtct gtataacatS cactacagtt accaggggacccaattttca taaattgctc 64021 cagcatcaca ccctcctctt acagagactc ttcctgtccattctaatccc tgacacgttg 64081 tcatcctcac tcagattccc caatagatag aaatgctgttaaatatactt gggtagagag 64141 ggataggcat gctcaaactc Sttttgggaa aaaaaattgcaactgtcaga aatgtgttag 64201 ctatcttcac agattatgaa acataaatat caattatctgttcacagcag tgaaagcctg 64261 catatttttt taagatagca agtataaaac ggctataagtaccatgttga gtagagacct 64321 accaccgaag tagagacctt cactgaaata gtgaagggatggttgtgttg cagggtcaga 64381 atgattaccc actggttgac actccctcca ggactaaaggcagcatcacc agcgtccact 64441 ctgcttctcc actgcttact gttaaggagc aaggcaatagcattatctaa gtaggaacta 64501 cttcattctt tcaaaacctg gaatcggctg atcagaagaagaaaaacagt ataaaaatac 64561 agaaagacaa gattctctgg ctgtcctaat gaatgctagctagctataat agcatggata 64621 agacatataa ccaaacatat aagatctatt aattaaggggaatctctatt tgtattaact 64681 catctatttt ctctaaaacc agtatttagt tatctcccatacattggcat attgtgtata 64741 tttgcagaat tcaaaatgtt actgatgtga cttttttccatcagacatct gagctatccc 64801 tcaactaata gttgagctgt tcctctatag ctcagtatctgagttagaac tcgaagtaaa 64861 aaggtataaa taagtcatgc tataaaattc aaaggcaatcagatgtttga gactatagtt 64921 atacagaaaa gtgtccttgt tcttgggagg aatacatcgaggagtttagg gatagagggc 64981 tatgatgtgt gcagttttct ctcaggtggt tcagagaaaaaaagacaatg tgtgtgtatg 65041 tatatttgtg tgtatgtgca catagagtga tactgcaaatgaggtggaat gtttacaatg 65101 agtgaatgca tgtggttttt tttttttttt tgcactacccatgaagcttt ccagtgtgtc 65161 tgaaataact tccagataaa acttttttta aaggcaatcagaacagtggc aatgaataat 65221 tcaattattt catatttgag ctgtctttcc tgccacccccaccccctata acactacata 65281 catacatagg ctcacataag tgacacttac tatacttttttgcctatgta atgagtgttg 65341 gcgaagtacg tgagtgggaa attcagtctt ttgcattcaagctctaagta attctaagag 65401 accttttaga taatgatttg acttttaaga caacaattttgaggttataa tatgatgtca 65461 tttcatataa acagaactgc ccttttgtcc tttcagatttgattgaaaat ggcccatgga 65S21 gtacaaccac ctgggaacat atcctcgaga aagacagttgaggcttattg cctaaaccac 65581 aggctactga aaacttggcc aaaggcgact actctagactggtgacttca aaagtgctga 65641 tgaatacagt gaaccatgaa agatgcatta tttgttttgaacattttact atgattgatg 65701 acccagattt ggcaagttag ttcactccca gtggagatgaaagtttcact gccttctttc 65761 tttcaacatt cttgttgaga cagatttcac ttttattggccaatgtacta acaaatcact 65821 tgcatttgca gcataatgaa ttcattagca acactgtggatcaatgctat acataatatt 65881 cctgagtcaa cattaactct taattaaact tccttattataatgcctctg gaaattttcc 65941 ttcaggctac taaacttcaa gccaacagtc taagtgttaatcagtaatca tgctagtaac 66001 tttttgagat gggtcagggc cagggccagg gtcagtgatccatcatgaat ggcaggttta 66061 aaaatggata tacgtgatca aaaataccca taagttgaccaaagcattct aacctttggg 66121 ttattctaac actcagtgta tcagccttct gagcaagccaattttttatt gccttaaaaa 66181 gtctaaaaga aagataaaaa cagagataat cagttttgtttggcaatggc aggggtgtgg 66241 gaaatgaaaa agaagaaagt taaagatgag tgaaaataggatagtcttgg cactttggta 66301 tgaaattgaa gtacttattt aaactgatga atattgtacagaaatataca gagagcagtg 66361 aggggctaag aacctaattc caaggtaact acaaggaaaaaattattgat ctggattgtg 66421 accagaattc aagagtttga ctgttgtcat tgttgctgttgttattaagt taaaaaaaat 66481 gagaaaaaaa tatacttttt tctagtatac ttaccaggaaaggaaagaga gaaggaggga 66541 gggaaggagg gagacatgga gggggcagag ggagagagagacaaagacac tgacaaagtc 66601 tggaaaaaga cacaaattca gatttaagat cccaaaatatgctacatttc tgcccccttc 66661 aaaatattaa actttgctaa aatacactgg aaattttttttattgtttat tttttaaatc 66721 aactagcaga ccaattagcc aagatgatat tttcatccttggtactcttt caggtgagct 66781 ctgtgggcta ccaccagaaa gtcctagcca accaagctctcactgggtca ctctgtgcta 66841 gctagactac atagcctcct aaaaaagcac Rcagccctgatgctggtcct gccaaagtgt 66901 ggtttgctgt catgtcctac aggctacccg tttcccagtgatttatcata ataaaccaaa 66961 tgggaatgaa atacccagaa atatgccata aatctactagtggtgaattt tcaatattct 67021 gcacaccatg gtgatttgtt aattcagttg gatctctattaaWcctctct aaaatgtcat 67081 taaRccatct gttttgactt gttaatcaaa caacttttaaaaatgtattt agagtatcag 67141 taacccttga attaatgcat ccatcagtaa taatcccctctccaacttta gatttatttt 67201 taagctctag cttttttccc caccattcca acaaatagcctgacttccaa aagtaagaat 67261 caatcacttc agttgtagaa atgtccagag ggtgccctactttcgaaaca ggaagtaact 67321 tgatccagca gtcccacttc ttggtatata tccaaaagaactgaaatctg gatcttaaag 67381 agatattttc actgcagcat atttgtaata gctaagatacagaggcaacc taaatgtcca 67441 tcaacagatg aatggacaaa ggaaatgtga tatataYatacacacacaca cacacacaca 67501 cacacacaca cacacacaca cacacacaca cacacatatacaatgaaata ttattcagcc 67561 ctaaaaaagg aaggaaatcc tgtcatttac aacaagttggatggaactgg aggacatcat 67621 gctaagtgga ataaaccaga cacagaagga caaatactgcatgatctcac ttacatgtat 67681 aatccaaaat agtcaaactg atggaagcag aaagtagaatggtagttgcc agaggctggg 67741 ggaagatggg aattggtaga tgtggtcaag ggttacaaagttttagtctt gcaaaataag 67801 ttctggaaag ctgctgtata acataagtat acaatcaacaatactatatt gtatacttag 67861 aaatttggta aaagggtagc tgttatatta agtgtccttaccatgaaagg aaaacaaaac 67921 aaaaaaaccc aaaaagatgg gaggaaactt ttggaggcaatgaataagtt tatggcattg 67981 attgtaatgg tggttttagg gtgtatacct gtctccaaacatcttgcaga ataaacatat 68041 taaatctaaa gtttccttgg gaaagaagat gagaaaataattgtcaagag actgtacacc 68101 aataaaatag tcaaggagta caatgaaaac ttaggaatcccatttcttcc tttgactcac 68161 actgtttgat gatatcattc acatcggcat ttggaagttattgagacatt tcaacataag 68221 attgtcctta tgatctatga tttgggactc tcttataagaaagtccattc tataccaatt 68281 tgttttgttc cttggtaact agaccttctt taattggatctaagaattaa tattcctatc 68341 tcataaagaa gactaaaagg atccaagtag atggagtttcaaaggtggtc cccataaaag 68401 catttttacc ttaagcaaag atgtcccaat ttttcctgcttgcttttcgg atgctgttct 68461 aaaaaaaatg taccttattc cttctactaa aagaaaacctatcaattcat tgattcattg 68521 aacaaatatt tattgaacat gtagtatgta ctaaacactgggtaagattc cagatatgca 68581 aagaggaata agtccctgcc cttaagaaaa ctcacagtgtggtcaagcta acaattctag 68641 agcaactgtg tgataagggc tccaaaagca catgcaggaacacagagaga ggcactctta 68701 gtcttggagg ggcttcctac agtgggcaat ttttgatgtgtcacaaaagg aggagctatt 68761 agggcacaag aagagcaagg gtgaagtgca gaaaggagtgaatagatggg gaggggaccc 68821 aggcaaagag aataacatca gcaaaggtgc aacttcatgggaacacagta catttggaag 68881 cctgcccacc atttgttaca gcagaatgca tgtgtgggtggagatgaggc tgcagagatg 68941 ggctggagag gattatgaga gatggatgac tagcaaaggaagttggtcat gtccttatgt 69001 ggtgggggac catggaaaga tttttattta taagacgtggtttattcttc attctgtggc 69061 aaaaagcaga tactatagtc tgaatgtttg tgtccctccccacattcata tgcgatgaaa 69121 cctcatcacc aatgtgatgg tattaggagg tggggcctttgggagataat taggtcatga 69181 gagcacagcc cttataaggg gattagtggc ttataaaggaggcccaagag agctgccttg 69241 ccccttccac catgtgagga caaagccaga ggcactatctaYgacccagg aaatgagccc 69301 tcacaaaata ccaagtctac cgtgccttga ccttggacattctagtctcc agaactgtga 69361 gaaataaatt tatgttgttt ataagccacc cagctcgtgatttatttatt tatttattta 69421 tttatttatt tatttattta tttagagaga gagttttgctctgtcactcg ggctggattt 69481 cagtgccatg atctcagatc actgcaccct ctccttcccaggtgcaagtg attctagtgc 69541 cttagcctcc tgagttgctg ggattacagg tgcttgtgaccacatttggc tgattttttt 69601 gtatttttag tagagattag gtttcatcat gttgcccaggctggtctgaa actcctgact 69661 tcaagtgatc tgcccacctc ggcctcccaa agtgctgggattacaggcat gagccactgt 69721 gcctggctat gatattttgt tacagcagct tgaatggactaagacagaag gaaaaatagg 69781 cctttctgta gcgtgagctg aaagacagaa aatagccataggtttctaaa gagagaaaaa 69841 gatatatgtt tgacaatatg tttcaattag tactttttttgaaatgcagt aagttaccta 69901 taccttattc catttctaat ctatatgatg gcatttaaatattgactagg tttctgatac 69961 agtttttgga gtctgaatgt cactgcatgg atgaataaaccaaacagatc ggattcatat 70021 gggtttgcca aatgacaaat ttttttccat gtaagattagaggaaaaact gatttcaccc 70081 aatttcctgc cttatttata ttagaactga agtcaatgctgttatccctt tgcatgttct 70141 tttgtattta aaatctgata tagaattgtg ctgttacagaagaaataatt taatgcattc 70201 tgccctctta tttctcatga gttttcattt aaaagaaaacaaaaaagaaa aaggacatgg 70261 acttttcttt atttctagac tttataaact ttttagttatcaaataaaca catgtattaa 70321 ttaagtaggt aaaatattgc atatattaaa aggtacttggagWctcatat gttgtcagtt 70381 tttcatgact caaaatttat gaaaaaccat ctaagactatcgtaatgaag ttcaattgta 70441 gatcttgaag tgacaaaaca tgaaccacat tgacttgaggtccagggctt aaaactgcct 70501 atttgagctc taggttaaaa gcatgtgtga aatgcaaagtctgacatctg cacagctgac 70561 tattgtgaag tctcagtatc attgttcata agtaaagcttgtccctaagc cataccacaa 70621 gcattcccag aggatataga tttcatYtga tttaacatgattgttgatat tggtagtgta 70681 tttcacgtga acttgtgaaa tcattgtcta tacagacaattgagacacta aaactttttt 70741 ttaagtattt tttgttaggg gctagatctg aggttttttattcatttgtt cttttgttca 70801 ctcatttgtt agttcagtta tttactcaaa aatgtatattgagcaattcc tatatggcag 70861 acactgacag atgctggatt cagtgatgag caaagcattcgtggtttcta tgcccaagga 70921 tcctatcccg tggagtgtag agtcaagcaa agaatgtcaacaacaaatga aaattttaat 70981 cttattaatg ttatatgcaa aaatgtaaca cctccaaaaaagacaattta tttcctatta 71041 aggaatgaag gcagtccact gaggaagatc aacatgtaagcacatgtaaa gcaaaataat 71101 caataggtaa tgtagttttt gaaaaagaag gggctttaagcaggaagtcc tgggtttgga 71161 cttgggtttc aaatctagct atgtactctt gggccacatacccaagtctt gtaagtctca 71221 gtttcctcct ttttaaagtt agcctaaaac tacctcgctcatctaatggt gtgagaatta 71281 ggtaaacagc caggtagctc atcaaattgt aattattataattgttattt tttgagtcat 71341 gaaagttttc acactgaagg gaacatttca acttgacctggaagagtaag tagtttatca 71401 ggcagaaaga atttgttagg gtattccaga gagaaggaacaaaatgtgca aattcaagta 71461 gtcagaatca aaatggtttc ttaaaggaat gacaaacagatcagtgcagt tggagcctaa 71521 agttatctaa ataatccgtt agaccttttc tttaactgataaaataaagc aagcattcca 71581 aaaatcaatt taaatccatc ttcctcctta tacctctccattccccatac catggaaaca 71641 cagttttacc tcttttacaa tgagttgtca gcatgaagagacttggttca ttaagattag 71701 caaacttcac agcattgaac aaaagataaa gaaaagtttatagcaaatac gttccatgtg 71761 aaagcatctc cagcaggttc agaagatgca atgaaaagactaaaacttga tatagggata 71821 tttcagtggt gctYgtacca gcgtcctaac attttgttctaccttagtaa aatctatccc 71881 ttattactca tcctatctgt agactctctt atggaacaagaagctttttt ttttttttcc 71941 ctaatcccgt ggggaagccc tctccttccc tgggtgattcctgagcaacc aggaaaacag 72001 cagtcacagt caacacagct agtacttgca gaaaatctctcttctgtgat gctctgaatg 72061 actcacagtt ccagatggcc ttgtcagcct ttatccgcattccactcttg gcaccattaa 72121 cctggtctaS tgtggttgca acaaataact tttcttgttatgaggaccag tgttttgaaa 72181 aatttaaaat gttataaaat taaaatgttt atatttggaaattatttcca aatataaaag 72241 gagcctgtct tcattataga ttatttggaa aaaatgcagaggggtgcaaa agaaaaatgg 72301 aaacaagcca tctttttttt acccataatt ctaccactcagagacaacta ctgtgaacat 72361 tttgatgtat ttatctccag tctttctaga aggtagatcactgtaagaga tttctgaagc 72421 agaagtacta agtcagaagg aataaacatt tttatacctcttaatgtgta ttgccagata 72481 gtttttgaga aatattacag caatttctac ttttagaagtactgcttgag agtgatgtta 72541 catggtattc tcactaacac atatacaatg ttataaatatgtccaaaaat aaaacttagc 72601 aactatgtta tctccttgtt tcaatttgaa cttattacaagagtgatcaa tttttaaatt 72661 taaatgttat ttttagcaaa gttatagatg caaatagtttagagaactaa gtagtaccac 72721 aaggattaaa aaaagagatc tcctgcagtg catcccccacccacaataca tacatgtatc 72781 ttccactccc cagagggtaa ccattttcaa atattttagctgattcattt gctatactcc 72841 actctatctc taaatgatat aactttacag ttacttcttaatagttcatt tttttgggca 72901 ttgcttattg atacttcata gcggaagata aggatttacctttgcaacac ttcacacttc 72961 tcatcaccca tccttctaat atattatagt cttacttagattgatagtca ttgtgtttcc 73021 atgagcagaa ccaaagaaaa actataagca gctaaactatgtaacatacc ctgattacct 73081 ttttttcttt gttttccttc ttgaaaattc ttgtcttttgttagtttctt tgttccctta 73141 gatgtccata tatacttatc actaattcaa tcccaaacttttcccatatt acaaaaatct 73201 aagtatggct caaacacatc aagaatttta ttaaattcatcttcttgaag aaatctcatc 73261 cagaggcctc tttgacctgg attggttatt ctccatgtctcttgctcagc tgtcactttg 73321 ggatgttctt tccttattat cccaagggta atgtttttagagtttattcc cttgttttgg 73381 tgagcacgtt ttccagtagc ttcctgagtc agtatgcatcagagatacat tttttaaagg 73441 ctcgcacctc agaaataatc tttattccat gctcactcttgatcaatagt ttggYtgagt 73501 atagaattct tggttgagaa tcactttcct tcggaatttaaaaggcatca cttcattctt 73561 ttctaggttt caaaacgaat cttcagaagt ctgatgcccttttgattttt gatccttcta 73621 tacgatacga taatgatcct tagaacaagt cacaagtctcttttttaata ctttatattg 73681 tgctaaatgc tccaattaat gagttctgga aatttttcttcaattttgtc ctcctgcagt 73741 tttctctttt ttctagaatt tctgatttta gatgttagatttcctgaact ggtcctctga 73801 tttcccactt tttcttctgt attttctacc tttttgtatttttgctccat ttccatagag 73861 atgtcctcaa tttacctttc aacccttcta ttgagtttttaacttattct atcatgtttt 73921 tactttccaa gagctctttt cctattctct gagttgttttttttttttcc atagcatctg 73981 cttttgtttc gtggaagcaa tgttaatatt tatgaatacaatatcaagtg tatttcttct 74041 cctctctctg atgatacctt cttcctgcaa catctgtacttttttatttc tcttcttttt 74101 tttttttttt ttttttgaga tggagtctca ctctgtcacccaggctggag tgcagtggga 74161 ccatgttggc tcactgcaac ctccatctcc tgggttcaagcaattctcct gcctcagcct 74221 cctgagtagc tgggattaca gttgcctact accacgcccagctaattttt ttatattttt 74281 agtagagacg gggtttcacc atattggcca ggctggtctcgaactcctga cctaaggtaa 74341 tccccccgcc ttggcctccc gaagtgttgg gattacaggcgtgagccacc gcgcccggcc 74401 aacatctgta ctttgaagtt ggtctttttc catttgttttggtttctttc acttactttt 74461 tctaagtatt tgataaatct tatctgtcta ctcatatttaaggctagagt actaaaaatc 74521 agattggaag ctctgtttgc aYaggtaaga tttgtcaactacatccactt tttgatgatc 74581 caactgaaca gtttcattca gaaatacctt gcaccattatctttgggttt tttctcttgg 74641 gttggtgaat tctccaaagg ttatatcaat ctcctgtccagaaggcttaa gcatagttgg 74701 aagctgacta ggggaagaag gttgggactt tcaaccttcggaattcattc aacaaatatt 74761 tgagcatcta tatttggcag cctctattct agccaccggagacacagtgg taaacagaat 74821 ttaaaaagcc ttgtgttatc atgaacacaa atatataagtgtttatttaa tccctctgtt 74881 ttcagaatgg cactcagtct tcaacctgcc taggccccccttgtctaggg acggttttac 74941 attcatcagg aagtataccc cttgtatact tgtttttcagcttccaaaat tttgttgctg 75001 ttatctccct tcccactttc tttgtccttg tcatttatgcctataaacat ccattgatgg 75061 ttttctgaag gcagaggagt taaatggata tgttaattactatagatgta atcagaggtc 75121 ttcatgttgt ttttttgttt tctgttttac tattctaacttggcatttat agatcttctt 75181 tgtgagtagt ctgaatttgt ctttaatgat ttaacattgaaacagtattt tccttactta 75241 tttgcataag ctctttcttt gttagtgtat tagccattgRcctgcaatat cttttaggat 75301 gaccgattct gaagcctgct gactgagttg cttgtggttcctgtcattta gaatgtatgc 75361 aggtcatgaa tcgcttagat ggaacagatg gaatgaaacttgctctaaat attttaccaa 75421 aataaataca tttaaataat gtaaagaaaa atcttcaatcactaggcttt gaacttataa 75481 cagaaaggta tctgctttta actatattaa ttcaacaaactttattgaac agttggtaag 75541 tatgaagcat catctctctg agaacaaaat accaaggaactgagtcccat cattacagga 75601 ttctcaggag taaatgggtc aatacatagt tataaaatgatgagggctga tctatagcag 75661 aagtacaaag ctgagaacaa ctcacttgac ctgctaagctggggaattca ggaagggttc 75721 agatctaaag agcagacatt tgaggtggtt cttgaaatatgagcacaatg atggttttac 75781 caggctgcaa aggtgacaaa gtgcattcca ggctgataaaagagggcatg caaaatatgg 75841 caggtacaaa aatgtgtatt taaggaagaa tgagaagtttctggaagttg ctgaagatac 75901 agaaaataaa gtggggggcg gggggggcgg gtggttggagatgaggagga gatggtaggc 75961 ttgattcaaa ttatggaaat gtatttataa tgcaccaacttgtaaagaca ctgttgttcc 76021 ctacccagca gccactagaa tttgttcagg tgtcaggcggttggtgcttc aagggagatc 76081 aggcccctcc caggaccagg atgaaccatg attacttcccagccaatttc atagcatttc 76141 tcttgccaat gaatggctta gatataagca tatgatgaatttgtttctaa tacagatctg 76201 agaaagaaat ctgttaagga acttctggta aagtttttatctttcttaaa aagtgacaga 76261 agaaaaggaa gttgtataaa tatacttaga gctgctgtagtcatcttgag accataagag 76321 gataaaactg agatcaaaaa ctactgtgct gaggatgggtgactagaaag aaggaaagaa 76381 tctggattct tgatgcatgt ttgtactact agaatcattctatcactacc ttcttattat 76441 ataattcaat aaatctctta ttatttaagc tattttaaatgaagccttct gttatatgCa 76501 gcctaaacaa atatactgat tcaagctccc agcaaattctgtcacacata attcaaggaa 76561 cgtttgttaa taacttcata ctattatatg gggaataaatgattagtcaa gaaagacttt 76621 ctgttttgaa aaattctgga aagatgataa tacagtatgtagatttattg tcatggctaa 76681 tatacatcta catataacat gaattacctt ggagtagtaataaaagggca gagtaataac 76741 ttgtgcagag ccctagacta gaaaagttga aaatataaaatggaggaaaa agtgagttat 76801 gagattcagt aatatgaaag tataagaaat aaaagaatccaaaagccata gaaggttgaa 76861 actgaaaaga attttagaaa gcgtctaggc caggtgtggtggctcacacc tgtaatccta 76921 gcactttggg aggccgaggc aggcagattg cttgaggccaggagttccag accagcctgg 76981 ccaacatggt gaaacctcat ctctactaaa atacgaaaacataactaggc gtggtggtgc 77041 atgcctgtaa tcccagctac ttaggtggct gaggtggaaggattgcttca acctagggag 77101 gcagaggttg tagtgagcca agatcgtacc acttcattccagcctgggtg acagagtgag 77161 acactgtttc aaaaaataaa aagagagtct agcctaaaccccttaattat acagattgaa 77221 aaccagtcct ggagaaattg gaagtcctca aagagtttaccattaatggt ggaaccagga 77281 cttccacaca atgttctgac ttcctttaga gcactttttattatatcata tagttcctta 77341 cttctcacta ttaatagaga ttattttact aagagtaagtataataagag ctgatcagaa 77401 gacctgagag acataatgga gtttcaattc taatttgctgttggctcctt gaaatgcttt 77461 gttgagaagg agtaaaaagc ttacaaaaca cacacacacacacacacaca cacacacaca 77521 cacacacaca cacacaattt cttaattatg tctaccatgggcatgggatg gtaggaatgc 77581 catgtaacta gtttccatgg atggaaacca cctaatgggaaatagagaaa aagaaaaaga 77641 aaagaaattg tgtgtaaatt ttcagctcat tttggcttagattacatgat gaaacttatt 77701 tctttgaaaa actcatttga ttttgaatta gattatatggaacttctcct ttgtcaattt 77761 taatttcttg tcttgtttta gtttgctttt ctgagcagtagatatcctcc caattggaga 77821 ttccgcaaaa ttagtactta gtcatcttgg ttcctttcaagtagaaaagt ttcttttaaa 77881 tacttcaaat cattggtatg cacatttgtt gcaaaaaaaaacctcattct ggaaaatgtt 77941 gcttatttta ttatccaaaa aataggtatt tattaatatactgcatttct tttaaataaa 78001 ataaattaca gattttcttc cagcatccac ttatttggaattgggtacat atgaagaaaa 78061 gacttcttat aatatggaat tatttcaaaa gctattattacttcttataa atataaagca 78121 tacaagaaat tctcaccatt ttctaagatt tacattagaaagatataagt attgtctttt 78181 agaatactgt ttttattttt ctaaatattc cttataacttacttattaat tttatccttt 78241 ctttgttata aatatccatt ttttttaaag acccatactatcttttccta tgcaatttaa 78301 cctaacatat tttctgtaaa tgaggttatt tagttatccatttttctacc tcctgttgct 78351 tggcagtcct caaaaccatt gcagatcttt ctagagagcagtttttaaga acagtgcttt 78421 acttttcaac tcttgatcaa cctgatgaga attctttgaataaaccagaa ggaaggctaa 78481 agagacaaaa gaatactata gtttccttaa agttaatgagccagaatcct ttggcccact 78541 ttggcattga acaatgtgaa taaaatggcc actctcacatcagtaattta cattttatac 78601 agtgtgtact ttacactgat atttacaaag aggaaaaacaacagtttttt acatgtaaga 78661 ggagctcctt ggcagataaa agaaaaactt actcccattagtacaacact ggagtttaaa 78721 tctagagaac aagtacttca gaacctagta gaaaaagtccctgatttgta ctttgtatga 78781 acaattaggt ttgagaatat tgctatataa tcacagaaaatactggctat tatactacgt 78841 gaagtcttca gtgacaaaga gagaaacaga aaaaatagttaatgccataa caatgaaaat 78901 gtggaatgtg aggaacgccc agtgaccgta aatatctaccttaataagcc tgacaatttg 78961 tatacagttc atgcaattaa caagtgtgat aaatatgattattactctca agttgaaatc 79021 aattttcttg gctaaaagca aagaattgtt attgagcagatgggattact tttctttgcc 79081 aatttatctt cataaaactc aatcaatgcc ttgccttaaaaatgtcaaat aactcagatt 79141 tcaggataac acatacaaac taatctcttc tgcctgtctctacctgatca ctccctactt 79201 caaaagctcc aacaacatag tataactgac acaaatcaaagcaaaaacta tttttctcac 79261 tgctacatgg tcttatcaat aatccagtat ttcaatatctaataaaagat accacaattc 79321 aaagcagtaa tgtgccccaa gctaccgatc cacaacatatatattgatat ctaaaaatat 79381 atggatataa cttgttatct actaaataag tttctttgcagtgtggtgtt aatagccaga 79441 attttaaata cataaaaact tgggttcaaa tcctaactctgccacttaaa tgctatatga 79501 caattggtca agttacttaa cctctctctg gtctcaagtttcttgtctgt aaagctgtaa 79561 tagcttttag agtgctcttc tgaatattaa aaaggcattatactcttagc ataatgtcta 79621 catagagtaa atatttaaca acaaaataac aaatattaggtgtgttattg gagaattgga 79681 gttttaaata aaggagttgt ccctaaggta cattcaatttaactatgata tttaaaactt 79741 caatttttca cattattttt ccaggaaaat atttcactcttatttctcca taatttgttt 79801 tgaattgtaa aagagatttg caagcggctg aagcagttacactatactgt atatctgcag 79861 gatgggcaac tgattttttt tttcttcttt tttttttttttttgagacag agtctcactc 79921 tgttgcccag actggagtgc agtggtgcga tctcgctgcaacctctgcct ctcaggttct 79981 agggattctc tgcatcagcc ccctgagtag ctgggactgcaggcacacgc caccacgcct 80041 ggctaatttt tgtattgatt ttcaagattt gtcaagagctcctaatattt tcttttctca 80101 aacacatctt aggactacca tgcatatcag tcagtcagctactctggagg tctgctgcaa 80161 ggatacacac cttccattcc cacccatcag atacttgtctcctttctccc ccactccaag 80221 acagcaacct tccagctcta gataaaaaga gctagtctttcttgcataag aacaacaaag 80281 acaaaaacca ttttggtagt gtatactgca gattttaaccaaaacttctc tgaagacctc 80341 tgatttccta aaaaacctct tcatcccagt gcagttgctctttcctaaat gagcaaccgc 80401 gcatctggtc aaatttggtg aatattctgt gctccagaaacaactgattc catccccttt 80461 aatctcttct tctcttatat ccatctccct ccttgttgtttctctccatg tttacaacta 80521 gttcattgat ttaaaaacca atgtaataca caaggaagtatgcacgacaa tgtttagtgt 80581 agtatggttt gcaatattaa aaaaggccat cagtaagaaaatagttaaag gataaacttg 80641 atatacatac tatgctgcaa tttaagaaaa ctggtatagatttatacgta ctaaaataaa 80701 ataatctcca aaacatagta agtgcaaaga tcaatcacaRaataacatct gtagtatatc 80761 acttttgatt tttaaaaatt acccctaatt ctaaatgttcatatatataa atattcagtt 80821 aaaaattctg aaaagatact gctatggttt gaatgtttgttccatccaaa actcaggctg 80881 aagtttaatt gccattgtaa cagtattaag aggtaggactcttttttttt tttttttggt 80941 gacagggtct cacttcaaca cccaggttag agtgcagtggcatgatcata gctcactgtg 81001 gcctcagctt cccaagctca ggtgattctc ccacctcagcttccagagta gctggaacta 81061 caggcacatg ccaccatgcc cagctatttt gttgtatttttttgtattga tagggtttct 81121 tcatgttgct caggcaggtt ggtctcgaac tccttacctcaagtgatcca cccacctcag 81181 cctcccaaag tgctgagatt acaggtgtga gccaccacatccagcaagag ttgggacttc 81241 taagaggtgt taggtcatgt gagctttgcc ctgatgtaagggttaatcct gttatcactg 81301 gagcaggttt gttataaaag agtgagacgt ttggtcccctttcctctctc tctcccaatc 81361 tgtctatctc tctccctttt gctttctgcc atgtggtgatgcaacaaaag gacccttaac 81421 agatgccagc accttgattt tagatttccc agcctccagaactgtgagcc aataaatttc 81481 agctcatgat aaattaccta gtctcaagta ttctgttagagcagcacaaa atggattaag 81541 gcagatatac accatattgt atccatcact aagtggatactttcaagaaa gacagcaaga 81601 ctaaggagca tgaaaaagga ctttctcata ttctgaattttaaaaatatt ttatgacatg 81661 aatatattta tctattacct atgtaataaa actagacattaagagaaaaa tctcatggta 81721 gaaatcttag gaaatagaga aaaattagag aaaattaagaaaaagcatgc ataatttcac 81781 aaaccaatgg tagccactgt aaaaaaatgt tatttcattctaatacattt ctacacacat 81841 atgcacacag tgcatttttg aaaacataac tgagtcttttctaattgacc tcttactcta 81901 caaaatttga aatagccaaa acaaattttt gattcctataaagagatagc actagagctg 81961 gaagagatgg ctcatgcctg taatccctac actttgcatgactgaggcca gagaattgct 82021 tgaggccagg agtttgagac cagcctgggc aacatagcaggacccatctc tacaaaatta 82081 ataataataa taataataat aataagctgg gcataatggtgtgtgcctat tgtccttgct 82141 acccagaggc tgaggcagga ggactggttg agccctggaagttcaaggct gcagtaagct 82201 atgattgcat tacactctac ccagtgtcaa ggaaaggaaaggaaaggagg ggagaggagg 82261 ggagaggaga ggagaggaga ggagaggaga ggagaaggggaatgggcagg ggcaggggca 82321 aggcaaggaa aggggcaggg gcagaggcaa gggaaggggaaagggcacta ggacctatga 82381 gccaacatag gctatggctt ccactagtac aacttaaaactcagctgaag acatgacaca 82441 tagaaaaggg ttgctgtgtc tgtacacaca cacgtttcccatgttcccaa tcccagtctg 82501 tggtcagtga aagtgaagat tgcctcagag cctgcattatctcaagctac tgacctaatt 82561 gctaatgctt tactctggYc tatttcctgc Mtgacctcagaatgtgtcag tgtaaattcc 82621 atagtaagac agtagttgca cattttggag tgtggattctgctttgaaac acaaataagt 82681 gtgatgaacg tgaccctggc actctgtagc cctcagtttcctcaacgcta aattgatcac 82741 tacagttcct tctagtgtaa acagtcccac gaagaatcgtgctgaatgga tgactacata 82801 atggcctgct cctagagttc ttaaatcaat ccttaaaactggctgcaaaa tgacttcact 82861 cctttaacag tgtgggactt gtctctgttc tccagaatgctcaccagatg gtgaagaagt 82921 atctctctac ctaccaacat cgtcatattg cttcttgccactcgctactc aaaaaYtgtg 82981 aaaaacctgt gggagcaagt atcagctgaa aagggtggctttctccttag gcaaaagctR 83041 cagggcaaaa cgggggcccc aaacttctat cacgtcaaagacatgaaaca gatgatccct 83101 agttttgatc ttctttgaag tttattaaaa acaagcttgaatctgtacca atcctgacag 83161 gtgtgaatgt aagactcaca aatgagaaat gctaagctttcctaatgttc aatttaatct 83221 tatagaccat gaaacatgtt ataattaaag atctgaactattaatatgaa ggagatagca 83281 tatattctta ccccaaatat gactaactag gtaaaatcttcaaaatataa tcatggcctg 83341 attatcagcc attgtggagg agaattttaa aaagttcttcattgacatcc caagtttatg 83401 ttttagacac ttctaatcaa acatgaatag attaacttcaagtaaaaatt aatagtcaca 83461 ttattgatta tcatgattgg aaatgtattt gaccagaacaatgaggaaag agcatagagt 83521 cctgctatct tcaagtatga atttacactt ttaactgatgtcaagtcatc catagtatga 83581 aaactcattt atttttattt gaaaaattta aaaccatcaaaatggatagt gtgggagcca 83641 aataatatat aagcaatatt ttatgagaac tacaaaactattaagagcag taactagaat 83701 tcggttactg ccgttcctta tttcagaaga gaaagtcaaatgttttctga tatttaagaa 83761 gtaataactt cctgttgtto agttatgctt tctataattatatatgataa gcaacagaaa 83821 aataggtctg gttttggctg taatgtgtat attcaattcaatattaattt ggactttaaa 83881 tcattttgct atcaaatatt ttgaacctca tgaccaggaagtaatcaggt aaaaaaaaaa 83941 aagaaaaaaa gtgggtttgt tacctgacct atctcttaagttcaaacttt ctataggtat 84001 ttttctctac ctttgtccat atggttatac aattatcactagatgcttga attactaaat 84061 ggttttgtat atcttttcct caaatttata tttgtcctattattggctta gtctctcaaa 84121 gtattacttt tatgatattg ctataatggt cctctatagtataaagttga gcccacacca 84181 tttatcctgc cacttaacaa tgtccttaat gtagattatatggtttggcc atgccctaac 84241 caaaatctca tcttgaattg taatccccat aatccccatgtgttgcggga gggactgggt 84301 gaagataatt gaatcatagg gcagtttccc ccatgctgttctcatgataa tgaatgagtt 84361 tcacaagatc tgatggttat ataagcttct ggaattttccctgctggtgc ttactctctc 84421 ctgctgccct gtaaagaggt gcattctgcc atgattataagtttcctgag gtctccccag 84481 ccatgcagaa ctgtgagtca attaaacctc ttttctttataaattaccca gtctagggta 84541 tttcttcaca gcagcatgag aatggcctaa tacagtagtatatcacactt attaaagagt 84601 tgatagtaaa atagcactct cagtacaata agaatagaatagttcctttt catggtaatg 84661 aatagctttt cttcgacatc tataattaac aatgaaatcagaaacaaaaa gaagctgtct 84721 ccactattac tgaatgttgc tcagaaaatt ctaacgtaagcaaccaaaca agaaaaccaa 84781 atatgctgta caaatatttt taagagagaa ggaaatcattctattttaag ataaaaattc 84841 tgtaactcaa aattcaggag aatcaactga aaggtataaaaaaggtagac cattatgatt 84901 tataagaaag gtcaaacatt ataaagcatg tatccacaacaggttttttt ctatatacca 84961 gttagaaaat agataaatga tcctattcat aatagtaataaaacatatca aatatgtata 85021 aataaacaac gaaaaatgta aaactcttgt ataaatattattgcaaaaca tcactgaagg 85081 acatttttaa aaacttgaag aatatatcta gtttcctacatgggaagagt caatattata 85141 aagatatcaa gtcttagatt aatctatgag ttaaatgcagttacttttgc cctaaaatgt 85201 taaaaggctc ttaattatgc aattcgaaat gtgatgtctactttcttctt gaaagcattt 85261 ttcctggaac cttctgatga ctctaactta ggctgcctgctttctaggcc tactactaca 85321 cagttgtcat actgggattt attttcataa acatctgggttggttccaat gctctttgta 85381 tcctaggttt tctgtatagg attcccgttg ctttgttttgcttgaatata tcttcgagta 85441 atacttcctt ttaattagga agaatctgta ggaggtaaagttgtaaactt gagtccttcc 85501 atgtttaaat acatctttag tttgtcttaa cacttgatagtttgactatc aattttgggt 85561 tctagttctt cttcctgaga actttgaaac cttgcctagtctgcattcac ttctaatctt 85621 gctgatgaga agtttgatac ctttgaaaga tcctttttttttgttctctg gaaggattaa 85681 tttcttatac ttagaattcc aaaattttca tcagcatgtgtatacacatc tagatatgta 85741 tccttttttc cttgttcggt gttggatgaa acttttcaatctgaggactt gaattactct 85801 tcaggtcaag gatttttttt ctgttatttt cccacatcctttcccattcc tccacttcta 85861 ccacattctt catatctttt ttacttttcc atcRtattttatatccttgg ctttttgctc 85921 tatattctaa gatatttgct taattcaata ttcaagaatgaatattactt gaattgcatt 85981 ttatccaatc tttatatata catatatatt cagtattctNctatttatgg aaatcagatc 86041 atacttaatg gttatattct ccccctgaat atctctgaaaatttaatatt gacaaatttt 86101 tcccacactt ttcattgtct ctgtttcttc tggaaagcagaagataaatt gtctcaggaa 86161 aataaatttg tcctgtttat ttattagttc ctttttgtttaggctactgg tttttttgtt 86221 tggtttggtt ttttgtcttt tttcaaatgt ctggtgatcctttcttgact gggaatattt 86281 gtaatttaag aactaggagt ttcttaagta gttggcagggatatcataag gagctctgta 86341 gtacttataa actgcttata agcaacttaa tgcaatttcaatcaaaatct taatgggttt 86401 ttttgggggg gacttgacaa gtagattgtt tgtataatttagcacaacga tgtgagaata 86461 acccaggaaa ttctaaaaaa taagattaag agggaatatgttctcccaga tattaaaatg 86521 tatttcttaa aatccataat aattataaca tggggctttttgtctagaaa taggatgtaa 86581 agattaaaaa aggaactaac cataaacagt agaagtggcatttcaagcca ataaggaaat 86641 aatagattat gtaataccta ggattctgac aatttagaaaatctaggaaa agaacatcag 86701 agccctgaaa taaagtaaaa taaattccat aattattagagatataaata tatgatgtaa 86761 aatgattaaa tactagaaga aaatattggt gttggaaaagaattgctaag gatttataaa 86821 atatatttat atagagacta tataaacaat ataaacatacaggctacata gggactattt 86881 aaacttctat ttattaaaat agagtatctg taaaagtaaaaatagaaatg aataaatggc 86941 aaaaaaNtgt aaaaaacaaa cataaatgaa aagttaatattcttaatctc taaagaacac 87001 tctaaaatca ataagaggcc aggggtggtg gctcacacctgtaatcccag cacttcgaga 87061 ggcagaggca gtcagatcac ttgagtccag gagtttgagaccagcctggg caacaaagtg 87121 agacacctgt ctcctaaaaa ataaataaaa ataaaatcaacaagaaaaaa caaatcagcc 87181 cacgaataaa taataaaata ataaaaagca aatatgtataattgccatta gacacacaaa 87241 acagatttta atattcttga agctaaatta attcttattaaaacaaaata ctatgtgatt 87301 catcaaataa agagaacaat taaaatgata aaatatagcaacggaaataa tgtagaaata 87361 gagactgctg atgaagtata tataaacttt ctgcagggcaatatgacaat aatagcaaaa 87421 ataaatcttt aaaatattgg gcaattcttt agtttgacaattctaattct agaaatatat 87481 agtaagaaaa caacccagcc ttgctataaa gactctacacaaagatattc atcacaatag 87541 tgcttataat gtgaaatctg gggagagctt caaatatttaataatagaaa attgcttaag 87601 tgtagtatat tcatgtaagg aaatgccatt aaaatgttatatagagaatt gtatttaatg 87661 gtaagaaaag tgttcatgat gcaatatact gtgaaattttaaaagcagat tataaaatat 87721 taccttcagt ataatctcat tgttgttagt aaaactccttctctctctac atacacactc 87781 tcacacacag agacagaaaa attatctctc aatggcataattattgacag ttcgttttct 87841 cccccttgct tattattatt ttgtaataga tatgctatagccactcaata aatatttgtt 87901 actaatttca aaaagtatgt attattatat ccaggttgttcatggaattt atcatgcaaa 87961 caactaccct cttgagagag tcaaaagtgg acactataaattatgaattc atataaacta 88021 tatcaggaca atgggcataa actgagtgtc ccaggcacggcaaaatgtgc ggataccctg 88081 gagatgtatc ttgactttaa gaagtttaca atctttcttggggatagaag attaaaaaac 88141 atgaaaccat tagaaataat ctcccacaat tagttttgtaaaagcaagtg cttaattgta 88201 cttgacaaac attagatgtt actggaggtc taagaagtaagagctgaata ctcgtcagaa 88261 aagctccctg gaggaagtgg ggactgagtg atacttttagaataggtaag aaaaagaata 88321 atgagaactt ttcaaatgga ggttggggtt gtagaaggaaaagaatcaag tattcagagg 88381 tagctgataa cgatgttgta tatataaaat caccctgacttttgcaaggg gtccgtctat 88441 acagaagagt aaaaagtaaa caatctatta acaatgtactatcactgcct ccactttaca 88501 caggagaaaa tcgaggcttt agaaggttac ataacctgccaagggtcaca tagctaaaag 88561 tggagagctg agatttgaac ctaaggagtt ggtttcagagtctatgcttt ccaccctatg 88621 ctgtactgcc cactacagtc aagctctggc ttcttgtataaatctgatgt cactctttgt 88681 gggactgctt ataacatttc ctcacccaga atccatacctcctcctttct gctctgtcag 88741 agctctttgt ctattcacac acagttcaaa gaataccttctccatcaaat ttttctaaca 88801 attccaatct tggaattctc tcctaccttg gagttgagaacaccactctc ctaaatttct 88861 gcctacttct cagacactac ttctcaatct ctttgttggattcttctttc tctatatttt 88921 tSttttttta gtctactcat tgcctgtgtg agtaccttcacccccaaaac tacaatacct 88981 acctaccatt gtgctgaaaa ccccagctta cattgctcttccacattcca taaatttcta 89041 tctggatgtg cacagacacc tcaaacttca tatttccaatactggcattt accctatctg 89101 cagtttttac tatcttatgg acagcagatg ccagaaatctaatcactgaa accagaaacc 89161 catgtacaat tcttgagtcc tccctctctc aagcctaaaatcctggaagt caccaagttc 89221 tgtctatttt actctataaa taactattag attcttctttcatctgaact cccttagttc 89281 aagccctctt tgtttctcat atgattattg tagtaggctctgaactggtt ttcctggtac 89341 ctgtctttcc cctgcctact ccaattctac ctccacatcaacatttctca aatttgaggg 89401 gtcatagact tccaaggaca tttccacaat ccctgagaagtatacagttt ccatatgaga 89461 aatcactgta gctaaaagtt aaatactttt cttcagttgattatgtatag ctaaggcaat 89521 aattcaagca ttagtaaata caaagccaaa atgcaggcacattacgcaga aaatgcctgt 89581 tcttgggttt ttaaaaaata cgttaaaaaa ataaggccagatgtggtgac tcccacctgt 89641 catcccagca ctttgggagg cYgaggtggg atgattgcttgagaccagga gttcgagacc 89701 agccaagaaa acatagggag accttgtctc tacaaaaatttttaaaaatt agccaggcac 89761 agtggtgcgt gcttatagtc ccagctactt gggaggctgaggcaggagga tcgtttgagc 89821 ctgggaggtc aaggctgcaa tgagccatga ttgccactgctctccagcct gggtgacaga 89881 gcaagaccct atctcaataa ataaataaga ccacaaaggaaaaaaattct tacagagaat 89941 tattgaagta taaattgtgt caaaattgaa gaatataataaaccctaaga ttaatagtcc 90001 tgtccttgag aaagatcatc aagtgatgcc cccatgctttaaaaaatatt ccaagctttt 90061 tagcataata tctaacattt cttagcagtt cctaataggttacttctctg tagttacctc 90121 tctcactcct tccccatcac ctatagtgcc acaatctgtaacctgtttga ggctctctca 90181 cacctttcat actgcaatat ttgctcgaac tattgacccccaaccttact catctttcct 90241 ttctaactaa ctaacttctc ctcatcctca aagccccagctcaagaattt tcatttctgg 90301 aagaatctct gattttcttc tcttctctag tcttgaaatggtatgtgtga acccacagct 90361 tgttgtaatg tcattgtcct ttctgtttaa caccctcagttgctggtcct tgaagtctgg 90421 gactgtctta atctacttta aagtcttcaa gccaagtacaattcctaggc atgcaataca 90481 tgtttgttaa atagatggat gacctttatt tatatttatctttccctttt ctgctacaat 90541 tagttaactc attcatttct tcattcacta ataaccacttatctgttatt ttctgggaat 90601 acaaaaattc ataaaacatc atccttgcct ccaaaaaacactttattatt gtgccatcct 90661 tctctctctt tctctttctc tccttctcta attatctagttgtgtaggta ttttcttcaa 90721 aggaataaca ggtactcaga ggaacactta tacactgttgatgggagtgt aaattagttc 90781 aaccattgtg gaagacagtg tggcaattcc tcaaagacctaaaaacagaa ctagcattca 90841 acccagcaat ctcatttctg ggtatatgcc caaaggaatataaatcattc tattataaag 90901 acacatgcat gtgtatgttc attacagcag cattaacaatagcaaagaca tagaatcaac 90961 ctaaatgccc aacagtagta gacaggataa agcaaatgtggtacataaac accatggaat 91021 actatgaagc cataaaaaag aatgagatag tgtcctttccaggaacactg atggagctgg 91081 aggccaatat ccttagcaaa ctaacacagg aactgcatgttctcacttgt aagtgggagc 91141 taaatgatga caactcatgg acacatagag ggaaacaacagacactgtga cctatcagag 91201 ggtggagggt gggaggagtg agaggatcag gaaaatcaactaatgggtat taggtgtaat 91261 atgtgggtga tgaaataatc tgtactacaa accccatgacacaagtttac ctatgtaaca 91321 aatctgtaca tgtacccctg aacttaaaag ttaaaaagaaataaaaaata aacagaatag 91381 tagggattca ttacaatatc ttctctcctc ctcataattcttaaacctgt gtagaagttc 91441 aatacatatt tgtggaatag ccttattacg cctttagggaatggagcaat gccttgccta 91501 ttaatctata cttctatcag agtttcctgc tgtatggaaaacctgagtgt tacacttcaa 91561 acaaatgagg caatttatac atttttgttg ttgttgttgttgttgttttt ggcataaatg 91621 tgctaagtga gctggagttt cagaagagaa actgaatgttatatgtgaat ccaggtcagt 91681 agcttggctc tgactacaat actgatttca ttcaagataaggaaactaat tggtatcaat 91741 tttagttgac attatatgtt agttttataa tattcaggaatttttgaagg attatttgat 91801 ttttattttt atagatgttc ttgctcttta acactaaataatgaggaaag ataatagggc 91861 taacatttat taagcattta tgttggacag gcactgctttaagtattttt atatgtatca 91921 actcattgaa ttttcacaac tcttagatct atttgatagctggggaaact gaggcacaga 91981 gaacctcaag taacttgccc aaggtaagcc agctagttagcatggaccag tgattcaaaa 92041 acaggcagtc tgtactcctt acaactgtat tatgctgcctataaaaggac cactagacta 92101 ataattatga aaccagattt ttaatcttaa attagccatataatagtaga gtgcatttaa 92161 ggatgtcatc tgagtttcat tgtccttgta ccccttacagcctgtgagaa ggaaccttta 92221 tatagtcttt cataaactgt aaagtgttat aaattcaaagatttcttata atatgttaaa 92281 attactttta agagttgaga agtccaaata aaagcagagagatgagagga aacactagca 92341 ctaaatgttc agacacccac tgaccaYgtc aagctgcacattttgttgct aaacctaagg 92401 gagagattag atatgaggag agtgggaagc aagaaggtatgaagtctggg tatttaggga 92461 ctaatagaaa tgattttctc gattagttta agaacattagcaattactta cgggataagc 92521 acagttaaag gaatttctgg aaggaataaa actataaaaattagtcaaat tgttaaaata 92581 tcagaactgt gtaaaagcat ataaagtttc atcatatctttcttttgaaa aacaaacaaa 92641 cacttcatgt tctagccagt tgctaatagt gatcagttgatgaatacacc tcatgtttca 92701 cgcatataaa attttacacc atgtctagtc cataggcattagtgtaggag tcagaataca 92761 acctgaattc tgttcgcagc tttgtgcctt gtcgataaagcagtggacat ctctccacag 92821 ggagatatgt ttcctcccgt gatatagagg atgttgattcacttgtctcc tctctatttc 92881 gaaaagatgc tctgaagatt agtgagacaa tggctgtaaagCtctttgca ctgtttacaa 92941 cataaatcat acacagacat actggcatga gaaacgctatcatcatctct tttatacatg 93001 agaacaacca taaggaaaac aaacctcaga gacagtcctgtattataaaa atgttgggta 93061 gagatgccaa aagttgtagg gtaggaaaca gcaacaacaacaacaagacg gaatccaaag 93121 aatatttgca ttctataata tattctacag agtYtcagacgggaccttcg ttgagtgctc 93181 ccaagtatta tcagtagcca tgtttttttt ttctttttcttttttttttt tttttttttg 93241 gtaaaaggga taaatagtga tgtcaggaga tgctgcaaaatatattccag tgataagcat 93301 atggcaagca attaatggat gaattcgcct ctcttattgccatgttgtga caaatactct 93361 aacttatttg gcctttggct ccctaaaggg tgctcagaaatgctgtttag aaagtacttt 93421 tcatgaagag ctacaacaac aacaaaaaca aatacaattcaaatatctac taaacatatg 93481 aaggagtact cactctcatt agtaatcaga gaaatgcaatgatcccatta tagagccctg 93541 tcatttgaaa aaaaagaaag aaaatccaag tgttttttattagaacgtgg agcaacagga 93601 attctcatta ccttctcaac cactttgtca aatgctttctgcttatctgc tgaagttaaa 93661 gatatgcatg acccatgaat ccactcctag gtatattcccccacaaaaat gtacgcttat 93721 gtgcatcaag atacacgcac ataagtgttc gaaatatctttattcataat atttccaaac 93781 tggatatgtt caactgcata aagaaagaag attggacagtaaattgtggt tcctttatac 93841 aacagaatgc tatatatcat gatatatatt atgatgtgcaatcctcagct acatacaaca 93901 ttgatgaatt ttacaactat aatattgcac aaaaggagcaaaacacagga gtatctacaa 93961 gatgagtcca tttacataaa atgcaaaaca aactaaattgaactgttgtt ttggcatgca 94021 tacatacgtg gtaaatcata aagaaaagta ggaaaagtaaaagtaaagtc aagatagtgg 94081 tgacctatct ggagaggatg aaacaggaaa ttatagggtctttctgaggg gtgatcatag 94141 taggtattta ctttagaaca atttgttaaa cttaaaatttaaattttaca tactttctta 94201 aattttgtta tatttcacaa tttaaaaatt tataaaatgataattttaca aagcctaaaa 94261 gatgaattat ttgggggctt ttacttctct ttcatctgtacaactgctga tgaaaatcac 94321 caggtgtgta taacatgaag catctcctta aatgacatgcatttattggg aaaaaacaca 94381 aagtccaaat ggtaaaatgt tagcaaggta aaacactgaagatttttctc cccaaaactg 94441 ccaaacaagt ctggtttaac tgaaattgac agcaaagacaacagagcaca cttaattgaa 94501 ttttaatctc agtgatctct aagccatgtt tgaaacataaacagacatgt cagagttctt 94561 tatgatgttg gacatgaatt cattttcata attcaaaggcatactttgct tatgaatctc 94621 tttaacaagc tttggctgaa tattcttttc ttccagatgtttgctgttgt atacacacag 94681 cttccatctc tttgcagatg atttaaaaca tagttagaaagagtacattt atttaacaaa 94741 agagtaagag tacagaattc aaatagatcg tctgtgggtgtatataatga tgagtacata 94801 agtctcatgc agtcctcata aagacagcat ctattaatctgaagaagttt ctcttactaa 94861 tttgcaaacc taaaagcatt ttacattact aagatattaataaataggtc aattaatact 94921 tttgatagag tagagagact cctaaataat ggacatgaaaaatgattttg taaaagtaYg 94981 gtttcatttt aaatccctgc ttaagcaatt tgtaggaggcaagatagtgt ggcagttgag 95041 aggatggtct ctgaaatcag atctgaattt gaataccacctctaccattt actaacctaa 95101 acaagtcata ttacttctct gtgtctcaat agctacttcctgaggtgttc ttacaaaaac 95161 acaaataaat aatccatgta aagaactttg cacagtgcaaatacatagtg agcacgcatt 95221 gagttttatt tatcatcatc atcatcttgc tgtacttttgtaaactcacc ttctcccgtc 95281 tttttctact ctaaggtgct cttccaagct actttcctcctcagctatcg ttcccctgga 95341 tttccagtaa gagagggtga aatcatgcct gcttatcttctttctctttt actgatactt 95401 cactgaagtg aaagtaaagg aataaaaaca caacacacccacccacaaag gcacaaataa 95461 taaaggaaac aagatcagca aaggagagat ttctactgattttttgaaat cagaaaatag 95521 ctgaagtgcc ttagcataat cgaggaaatg gttacggagagtaaattcca agagaaagtg 95581 gactaatgtg ttctcccgaa tccagaaaga cttactgcttggaggcagca gaattaagga 95641 aggcaggagt ttagcgaggg gctgaaagtg tattaaaattatatggagcc tttggatcca 95701 aactaactac ttcccttacc tactggaagc ggagtctttcttttttggag gaaatcggct 95761 agagagattt gaaactgatg atactaggca ctgtagcaaactaggagagg ctgcaggttg 95821 cgaaataggt gcttatttga aagcctatag agtttgcaataagaaaccca acttttttat 95881 ttgtcatgct ccccagacat tagcagtcag ttatatgccccaggcgcaaa tctcgcaggc 95941 agaaagtggg gtacccccta atcatgacct tggggaaacatttcaaagaa cacaccggct 96001 ttgattgaac aacctatgat gcaacagtgc aaccctacagttgacgagcc ctatccatga 96061 tcacagaact tctgacattg tttcttgcca tcattgtcattgttgctttg ttttgtgtgt 96121 gtgtgcatat gcacctgtat atgtcttgct tttaactaagaatgaacagc ctcttaagga 96181 aactctccat aatgaagaag agaaactaga agaagcaaacatcaactgaa gaaagaaatc 96241 tacaggaaag agagagacag aggaaaacct caagaggataataatctgtg accttagata 96301 aaacacactt ctgacataag aacatatacc attaaaaaggagaggatgag gctgggcatg 96361 ttggctcatg cctgtaatcc cagcactttg ggaggctgaggtgggcggat catgaggtca 96421 agagatcgag atcaccctgg ccaacatggt gaaaccccatctctactaaa aatacaaaaa 96481 ttagctgggt gtggtggcat gctcctgtag tcccagttactcgggaggct gaggcaggag 96541 aattgcttga acctgggagg cagaggttgc agtgagccaagattatgcca ctgcactcca 96601 gcctggtgac agagcaagac tccgtctcaa aaaaaaaaaaaaaaaaaaaa aaaaaaagga 96661 gagaatgaga gtgctaaatt atttttaaat catgaccaaaataaacattt taatagtaag 96721 gttggaaaat aaaaatgtgt gtgaacaaat cttctagaagaccaaaaaac aaaaagacaa 96781 aaaaggaaga aaacaattaa gaaattaaag gatatgatctaggagatcca actaagataa 96841 tgagaattca aggaagaaag aacagagaaa atagagggcataaaattatc aatgaataag 96901 agcaaacatt tcagaactga aggactcata tctctggagtgaaaaggtct attgcgtgcc 96961 cagggcaatg gataaaaatg cagaccaaga caattgatcatgaaatgtgg gaacttcagt 97021 aaagaaaaga aggtcacaaa aatttctaca cagaaaaagatcaggatttc accaatagaa 97081 aatactggag tgatgcctac aaaatgttca aaaaaatacatttttaacat agaatccaat 97141 tgcagttaat ccatgcctga gacaaaaaaa ggtttaaacaaagggctgcc tctgaatagt 97201 atatagaaaa gatttctgaa cagaaaatct taaaaactagcttttatccc caacttggat 97261 actaaatata gagacaggca agaattaggg gtcttcttgtcattcttctg agactctgga 97321 gctcctgaga cttctttttc actaacacca ttgaatccgggaatcaacaa gctgcacaaa 97381 gttataaaga ttgccagact tcatgtttct ggaaaaaaaaaagtactatt ttttcataca 97441 gctgagacct ctgaaaagat ggctcaatgg gcaagatgccatgagaaatc aaagccaaat 97501 actcattctg aagtcagttt tgctcagact ttgtatactgccactcaatt tttcaagtca 97561 tcagaaacat aactaagaat gagtgacttt ttgctatYaactaatgaccc accattcaac 97621 gatcttgtat gacaaagtag aggagtatct tcttatttttaggcttatta aattacaatg 97681 gaaaatattc attaacaatc tcataatgag gcattctagatgcttattct tacctaaagg 97741 gaatggaaca aaaaataacc aaaaactaaa aagatgaagacaaaagcctt tcttggtatt 97801 atagtattct tttacaaagg atatggttta tcatagtaattgattccctc tgttacacta 97861 gagatactat tatattaaat gcctaaaggt taaacttaggtaacagtgga gctatagttc 97921 tttcagaaat aaaataatta aatagctatg tatgccaagaaaccttactt tgaacatttc 97981 tattgtacca tctcattatt gctggctgcc tgattagcaagcagtaaact tgggtaatag 98041 ctttcctctg atatcacttc aagtctactc gagttctagaattacccaga ctaccaggac 98101 tttacttgca caatatgctg aaatgtttat aacattatgctttcccctcc taattctcaa 98161 gaccattttg ataactggct aagactggat aatatcaacaaatatgaaat aattataaga 98221 atcattcttt cttaaaataa gaagagtttc cagaaacagaaactaaaaca gaattcaggc 98281 taagctgcta aataggctat ctcagggatt ctcagctcattcttgatgag gatagattgc 98341 tttttatttc ccttaagtgt tccgtattta tttcacaaatattacttaac tgtgtaccat 98401 gtacaaagcc gtgtgctagt taagctggtt actgtggaagaaataaaggg agtgggagca 98461 tctagctttt ttcttcaaag gagatggggg aatctgtgggagttagtggt aggggcacct 98521 acagaggaaa tWgctgaaaa ggaggagctg gggaagatgaggatgaggaa ctaagttgtg 98581 aataaaggaa gggcagagca atggtttttt taataatattggaatgggat ttgtttttag 98641 cactgctccc ctttccctta acagtcccct tgagctacaaataaatgtaa ctggcaaagt 98701 ggataataag acttcagaat cttctagcta accatgatcaatgtgtcatg

Following is a genomic nucleotide sequence of a GPX3 region (SEQ ID NO:5). >5:150384801-150484800 1 ggcttatgtc tacaatccca gcactttggg aggccgaagcaggcagatca cttgaggtca 61 ggagtttgag accagcctag ccaacatggt gaaaccctgtctctacaaaa aaatacaaaa 121 ttagccaggc gtggtggcac acaagaggct taggtggaagaatcgcttga acccaggagg 181 cagaggtttc agtgagccaa gattacccca ctgcactccagcctgggtga Yagagtgagg 241 ccctgtctca aaaaaagagg aagaaaggaa gggagggaaggagggaggga ggaaggagca 301 catacttgtc tgaggatcag taataatacR tataataataacatctgact ttcattgaaa 361 acatattatg tgccaggtag tattctgagt gcttaaatctattgattcat tctgtcctca 421 caactacaag gtagtcactg ttactattct tttttatagaacagaaaatt gaggcccaaa 481 gagatcaaat aacttaccct gtccctaatt agtaatggagctaggactca gaccatggta 541 agctggattc agagcctttg tacttaattg caatgcctcttScatatgYg ctaggtaggg 601 aacaaggtga tagcagaatt taatagtcag gattgatgcagtattcctga actagtatag 661 aatgagctag attaaaaact cagcaattag aattcagtagcgaatcaaat cctgagataa 721 gtaggatttg gcttatcctg tggccaattt tcctcgtattaaacatataa attcatgggc 781 cttcacaccc ataaaagaat aaattagtat tataatttttaaaatatgat taaaattagt 841 gctttctgaa gtactggtta ggtgtttctg tttgcaaagagaatttatgg ttttgtaaga 901 ttagtttaat gttgatggtg gaatagacaa gagccaaaagacactagaag caaagagaaa 961 aatgaaaatt aaactatagc aatccagcag ttagttaataaggctgcagc tcagatattc 1021 ctgttcctca tccagtcctt actttttttc tccaatcacYgttgtYaccc tggtcagttt 1081 taattctgct aaataagctc tccccactga ccctgttgcaactgtccttc tttgttcctt 1141 tttactattc ctcaagatta tttagtgttt ctgcccagtttgcaaaatcc tatttgtata 1201 tagccatata tactttgttc attgtcatat tctgtttctgtttctactac tgcaattttt 1261 aaaaaatgtg gttttctttc ttgaaatgct cagggggatagactccattt Rgccttgata 1321 tttcttgtag gccatatgta tattatgcat gaaagtaaagattcaatctc ataatatgac 1381 aaaaagttat gagtcataat acattagcag atatatttaccagtatatag ttaattgtgc 1441 ttctttctag aagtctggca agacttccaa ccacatgcagaggcaccagg agaaccaata 1501 aacacttttg aggcatgttt cattaataga ccagggaactgagacagagg aaaggKgtga 1561 caactgtaat gctcttgaaa atatatttca tctgagcccagactttattt caaaaattcc 1621 aaaaatgtgg ctgttatggt aggaatttga actaYttggatttatttagt ggcaatagaa 1681 gatatgccac aMaaaaaaag agcatagtga atgtgaaaaagcctccttcc agaagtcaga 1741 cctcattatg caaaagagaa ccataagaga gaaaagcccttaatatgtac taagtgtggg 1801 aRaaccttca gcagggaatc aaatctcatt atacacctgagaatccatac tcaagagaaa 1861 acctacgagt gtgttgaatg tagaaaagct ttctcccacaagtcacatct gttgaacgtt 1921 agagaattca caccagggag aaaccctatg agtgtggcgagtgtgggaaa gcctttttcc 1981 aaaacttaca cctcagcatt tacaggagaa ctcatactagaggaaaaccc tatgtgtgtg 2041 atgaatatgg gagagccttc Wgcctgaagt cacacctctttgtacatcag agaatacaca 2101 caggagagaa aWcttatgtg tgaactaagt gtgagagagctttcagtgaa gtRtcatgcc 2161 ttattagaca tcagaaaatt tacactagag agaaaccttatacatgtaat gagtgttaaa 2221 aagttttttc tcaaaagcct gacctcatac attacagaattcacacaggg aaaaaaactc 2281 atgtaaatgc agtcaatgtg taaaaaaaag ccttcagacatagatcagcc ctctgtcgac 2341 ataagaggac tcataaagaa aaacatccct gaacaagaaataaatatggg aatatcttta 2401 actagaactt gtagtgatag aaagccttca gtcaaaagcaaaccttatta tttatcaaga 2461 aatcttactg aggataagcc tgtttaatat atttataaaagcgcgcagtt atgtttctat 2521 agaaaacata ttccagatgt gattcctaaa tatttcttgaggatattaca gaaaatacat 2581 ttttattaga gaataaatgt tcactgtgga ccacaaaggcttttaaaata ttaataagtg 2641 aactagtcag aacaaactta gaaaacRata tgtggacaacccacaagata tgaatgttac 2701 tatatgtgcc actacttatt gctgtaagtc ctggctgcactacattttgc tatgcatcat 2761 ataaaataag ttatatgtgt tggaattgca tatgtgaatctaaaaatttg taaatataaa 2821 ataattacta tatatagaat gccatttact aagactttctacattaaata ttaccatttt 2881 tcccctaaag aaaatacaag ttataaccaa aaatacagtgttcagtaaga gttctaaagt 2941 gtaaaataaa cttttttctg ttgctttctg gtatgtatgacttctgctgc ttcttaatga 3001 gaagtacata aaggtgttaa ttacataaaa tatataaatataaactagat atcatataag 3061 tctcagtagc aaaagaaaat agtcatggcc tcttacaagactcactgcaa tttaRaaagg 3121 aattcttggt gggtacaatt acccttttta catactgttatggactgaat tgtaccttcc 3181 caccaRattc atatgttgaa gccctaatca ccaatacctcagaatatgac tgtatttgga 3241 catactgcct ctaaagagta attaaaataa aaccattaggatgtgtccta atctaatctg 3301 actggtgttc ttacaagacc tagagagtag gacacacgaggagacatcag gggcaggtgt 3361 acacagagaa atgaccatgt gaagtggcag caagagggctgctatctgct agccaagaag 3421 agaggcctcc aaggaaacca acccttttgc acattgatctcagacttcca gcctccagaa 3481 ttgtgagaaa ataaatttct gtcatttaag ccactcactctaatattttg taaggcagcc 3541 ctaggaaaca aatacaaaca ctactgctct ttttccctggagtactttgt aagcttgagt 3601 atacaaagta taagtactac gtaagccaat aatatgatagagtgcttgct atactgcacg 3661 gcatctctat ttggcatttt ctaattcacc ttgtgacagattagtaagag agatattttc 3721 ataaggattt tacaattcac aaatattata aagactttttgcagagagaa ttgttcattt 3781 ttaaaattcc atctaacagt aggggtaggt attctgtaaatttccatgtg agtatccatg 3841 ccattaaaaa tcaacttgtt ccttatttat aaattaaaagtggggcctca ttcttctaaa 3901 gcaaattttg acagggagtt aagattacaa ggcagaaacctacaagaggt gttaagagct 3961 cttattctgg atttagacag atgtagattc tgaatctgctctgagcctca atagcaccta 4021 gtttgttgga tcattgcaag gattatacaa tatcatgtatgtatggtgct cataagatgt 4081 tagctatttt atattattta aggatataat tcocattctagttatgaaga cagacaagga 4141 aacaactcta attaggatta tattagagag aactgtagggagtcatgtgt atcattctgt 4201 ttcattctca caaccatgtc agtctttcag actcaggtgtgggcctggaa ccactgccct 4261 gaattgcccc tctacatcta ttctataatt gcttgttcagaagcaatacc attgctgata 4321 ccccaaactc cttatcttta tctcatctac tgatgagacccattgcttgg agggtagtta 4381 catggaaagc cattcacaaa acaccttatt aactttgtgggtgaaaaaaa agtgtatctt 4441 aattgtatta agccactgag atttagggat tgattattaatagcaaatag caatcatttt 4501 ctagtagaaa gaaattggtc ctggaagtgt cctgctaatgtaataaaaac ctaaaatatg 4561 gcataggcct agtagttgag cagtgagaaa actgatgtcagaagtggaaa gatgtttatc 4621 tatgttttgt actagccaaa catttggttt gggccaggcgtggtggctca cacctgtaat 4681 ttcagcactt tgggaggtca aggtaggcaa attgcttgagcccaggagtt caagaccagc 4741 ctagacaatg tgatgaaacc ctgtctctat aaaaaatacaaaaattagcc agatgtggtc 4801 gtgtatacct atagtcccag ttactcaaga gcctgaggcaggaggatttc ttgagcccag 4861 gaggttgagg ctacagtgag ctgtgatttt gtcgctgtactccagcctgg gtgacagagc 4921 aagaccctgt ctcaaacaaa acaaaacaaa acaaaacaaaacatttggtt tgattgtctc 4981 ctctacacat gcttactttg ttgacagctc cagaagataattagaaaaca gagtattagt 5041 aatatttgct gttttactgg ctgtatttgg aagctattgtaagaaaaaga taaactcaga 5101 gaaaattagt ggattataag caaaaatgaa agaaaatagagtcctgaaat ttgagacctc 5161 atggagttta aaaaatttga tttctagact tcaaatgttaggagaataat ttaaatggtt 5221 ttgagtgtat caaagaccta ataaacattt gctagtgaaataaagtgact cagaagaaag 5281 attatattaa gaaactgaaa ccttctcgtt caagcttgataaccttacag tagtctgtta 5341 ttcacctacc ctagctccat gatggagaag ctcaattttaggcagctaca gccaagaaga 5401 ctggggctcc catacccaac ctagctccca cttgtagagcagaggctctt gccaaggtaa 5461 gagaggctaa gagtattggg acctcaattg ccctcctcctcagcttttct ctatagagta 5521 gatgtttcat gccaggaaag gcaagccaaa aaaaagatcattctcccagc tcccactaat 5581 aggatagagg ctccagactg gcaggagtac attaaaaagaYctcaggcct tcatcttcct 5641 accaagatct cacttgcagg aaggagtatc cctgctaagaagcttccccg gattccactg 5701 gtagggcaga ggtgtcactt caggaaaatc cggcKttcccctttgtcccc cgcttctgtg 5761 caagagtgta gatgttatat ccagggaaag aagcaagatgggagggccag aagctcctca 5821 gctcagctSg ggggctgact gtatttaatc ataacatgaagggttgcatg aattaaggca 5881 ttgttgaaaa tagagatctt agtggcaagc acttaagagaattctaatag tttcataaaa 5941 caagtagcaa caaatgaaac agcaagtcac ccggtttaacagagtgaaca agagaaagag 6001 ctaaaactgg aagtctgtgc atatgtctat gtttcacacattcaggacca accataacag 6061 gtactagctg aacttgagtg gaattgaaag aatttcccaagccacacgct gatacattag 6121 caaagagtgg aagtcttact agctcaagga gtgtaagcacaacccctgac caaacactgg 6181 ctaaatgtta agatattttg acccaacggt gacttctaggaagccaggct tggggtaaaa 6241 gctacacact gtggggtggg gggcggcggg gggaaatagagtttacaaag ttggtgcaag 6301 caagccacta aacaaactaa caaaacagag ccacatcaataatccctaaa ggggatgtgt 6361 cagaatccag agttgctaca atatattatt tcaaaaactattagagatgc aaaagaacaa 6421 aatagtataa cccttgtaaa ggaaaaacag catcaataaaaactgctttt gaggaggtct 6481 agatgttgga cctgtaaggc aaagatgcca aagaagctattacaaatatg ttcaaaaaac 6541 taaagaaaac catgttcaaa gaatgaaagg aatgtatggtgacaatgact catcaaatgg 6601 agaatgttag tagaataaaa agtgtttaaa gactcaaataaaaattctgg agttgaaMag 6661 tagaatacct gaaatgaaga ttcactttag gtactcaaaagtagatttga gctggcagaa 6721 gaatcagtaa gcttggggct cgatcattcg atattatacagtcttagaac agaaagaaaa 6781 aataatgaag aaaacctgag cagacttctg gtttctagtctgacatgtaa agagcttgga 6841 agttgtcaat cctatcatca caaccagaaa aaagtctgaaaaactggaaa ttaacaattc 6901 ttagatcctt caaagaatca aggtcacagg acaaaccactgccccagaaa ctagagagac 6961 aggtagatgc tgagaatcac aggttcactg acagcggaagtccaccacta aagtcaatat 7021 attttaggaa cacttaaact acaattgatg aattgctagagactagcaat aaaagtgcca 7081 gcattctagt tgatgaaaat gttgtgacca gaggcttacatgaacctaat cctgtattcc 7141 tcttggagca attattcagt atttactaat acagtgtttgtggtggattt atttaacata 7201 agtactgtga ataataaaat caactgtata tgaaatcttgactatccatt tacatgtagg 7261 agaatcaaca aatggtattc tccacaaatg gaatcaacccctagaagttc ctgagaaatc 7321 cctgttgagt gggaaaggac ctagagccaa ggagacaaggttggaggtga ctggtcctct 7381 tcctttgttc ttccactgaa attacttgag aagcatcatgacacatcctt atacacaggg 7441 tatggtggaa aaataattgg cagtcaaaaa gacttaagtgtatgaagact taagcatgat 7501 acacatggag aaggtgatgg tgtaaagaat gacaatggcgtctgagtaac attctgaagg 7561 aggagggaag ttcaaaaggt gggatggtgt gcctggatgagacacatgag ggagaaggat 7621 tttctatggt gcttctgtta gtttagcagg tagaatgcccctgtgaaggg tacaggaagc 7681 atgtggcaga gtgggtgcct aagtctgggt ttagggagactttagaaaca atttgaaacc 7741 cagggtctta ccctctgttc acctcataga acagtaaaatcccttggatc aggcaaactc 7801 tgatgaaaaa cccagactgc catctctctc ccagatggccaattttcctt ctggtgctgg 7861 cgaatgatga agttgtcttc aggaaggcag aattctgctgSgtgctctcc tcttctgagg 7921 agttatacca ccacacagcc tttgctccca gaaagagtcaccatcttgtg ttctaacatg 7981 gatcccttgt aagcccatac aaaccactcc atccatacttaactgctttc tccacaaagg 8041 caactgagtc taggagccaa tggactatct cactttattttttaaaattt acccagggca 8101 ctttttcatg ttctttcttc tccctggaac ttactagcattatggaacac aaggatgtgt 8161 ttgtggtcat cttctcttta caagataggt cacatagacccataagtagg ctgtgtatac 8221 ttttcttaca aaggaggtgt cacttacctt gggaataccagccagccaca tatgaaatcc 8281 cagacacagg agacccctaa gaaaaaggcg aggctgtctgtaactgcctt agcaactaag 8341 ccaccagtgg attcaactct taaaattaca gcaccatggaaacacaaaat cacagaattt 8401 tggatttggg taacactaga gagctcacat aattaaataccttaatagaa aattctcatt 8461 tactaattag aagtttttaa ccatgtagtg taaccatgtatacactattc tcaaagagaa 8521 tgggaatatc ttcttcagtt acttaatgaa aacatacaaggaatctgaaa ccctaacata 8581 ccagaatcca tgggacaaaa ggaaaattat ttgatcatgggattcagaag catttactgg 8641 atcaaggttt agagatgctg aaagtagaga atcagtgttaggttttggag ggaaggcaag 8701 ggttaaagga agacagagag agaaacagag caactcaacagcaaacacag gtatattgca 8761 gacacctgtg gaagcgggag acccgcttaa tgccaaaacccaccaccact tacaagcagc 8821 agtacttaca ggtatggctg ggagaagtct ggatagtgtggcttgctgcc cggcaggata 8881 tcaataagat gtacccatga tagggtggtt tgaccctttttctggtggga tgtcatcatg 8941 gtgttccttg ggcctttgcc cagtaagaca ggatagggaggtttctttag ttgggccttt 9001 gcctggtagg gtataataag aatgtttttt ttattgggcctttgtcttcc ttgtggtcag 9061 gtggccaggt aggatgtttc tcatggccca aacccccgtggaatgtttca ctttgaccaa 9121 ggtctgcaaa atagcagggg acttacaaaa tggtgccgtttgcactaaca caaaggtgaa 9181 tggcatgggg aatggtaggt caagaaagct gcttccttaatttatccctt ctcttgaaat 9241 gtcttcatta ttaagacttt cattttaact gcctttggagttaaaatata aaatatgaat 9301 tttaacacag aagtgtgaat gctggtattt ttgaaatcaagttcttataa tcccaggcta 9361 gtgacagctt gcctgggcaa ctaagtaact taagttcataagtaaacact aactctgctg 9421 aaactaaata actcatttta gcttctgaac attttaagcatttctcattc tggataagaa 9481 agagaaatgc tgaagcagaa tttatttcat ctgctttagatgaatttagg aatcccttgc 9541 aaggcataaa actaattttg tgatcctttg agtaatatggatttctgagg aaattctaga 9601 ttcagagaag tgctgggcca gattttggtt aaggcaatgacatggaaaaa tttttcaaag 9661 aagaggtaaa aatatgtagt ggagtttttt tgtttLttttttttcataat caggagtttg 9721 agagggtata atggaagttg ttaggttttg acgggaaggcgagggttaaa gaaagacagg 9781 gagagagagt tagcagctct acagcaaaca caagttttatgtctagcaca agacctgcgg 9841 aggttgggga ccagcttaat gccaaagccc actgccgcttacaggctggg gtacttatag 9901 gtgtgggtgg gaggggtctg ggcagtatgg cttgctgcctggcaggatgt tgataagatg 9961 ttcccatgat gagatggttt ggtctttgtc ctggcaaaatgtgatagtga tgttccttgg 10021 gctttttccc agcagaatat ggtaagaaat tcaggtggttgagcaaaatg tttctcatgg 10081 cctgaatccc catggaatgt ttcactttga ccaatgtctgcgaaatgatg gggggcttac 10141 aaagtggtac agtttggatt aacattcttg ccttctacttcagtctaaaa ggaagagggg 10201 cattgttgat tatctagctg cttcctgctg aataggggcactgtaatcag ggcttgggtt 10261 ttgaagcagt gggtgtccaa cttcagagtt gttttcctggagatgttgat accagacttg 10321 gtggggaaag atggtatcaa tgtgtttctg ggtggctgcctagacaaggt agttctgcct 10381 ttgggagata aagtgggata tgaaggtaag tatacatgggccaattgtga gtattaggag 10441 gaagattagg ggccctaaga aaggggtaac cagatatccctgtgccaagt gtagctgaga 10501 tgcttagtcc tgctaacagg ggaatgaagt gtatagcccacttggtgtgt gtggaggagg 10561 aggaaatagg gattactaaa gaacctgaat ggtttgattgttaggcaaaa tgttaatgtt 10621 tggagattga aacaccaggg tacatgttcc tgaccaacttgccagtaggc aaagaagaga 10681 gtttgttcca cacagaaaga aaagactggg ggttgacaggcaaagttgta agtgaaggca 10741 gtgagccatg agaagccggg ggttaaatcc tgaacaaacttttgtcccaa cttttttgtt 10801 tttccaggtt gaataactgt taacaaggat agtccctgttagggcctgat agggatattg 10861 gtggtgaagg ctgtctggtt ttagacagag agagagaaatgggtttttgt taacagccat 10921 tgcggtcctg acagggaaga agaataaagg ttgcagttgagggaattgtc tgtgcatttt 10981 tttagggagt tcccttaagt tggatgcatg gagaacagtgctggcagaga agggaggggt 11041 tgggaaggga tgttttatgg aattcagttt gtaacatgtttagtttacag gccatgagac 11101 gaaagagggt gccagccctg cgtgcgatgg tgcaggtaggtttgatggat tgcagggaaa 11161 ggtgtttagc caagcaagat tataaacagg ttttgggaagggactggttg agtaggtgag 11221 atgcaggttt actttggatc aagtttctgt ggttaggttaacaggaaggg ctgtgaactg 11281 ctggatttgt gtggacaaac aaatttaaca attggaagaaagaggagagt gtgactgatt 11341 taagaggtaa tgtgtgaggt tgacaaagga agcccaactgccagaggtta tgggatttag 11401 agtatcccac agcaaagaca aaaggggaaa aagaagatttgagtacRagg gaaattttgg 11461 aaggtRccct gcagccatag ctcctgggtt aatgtgaggaattggagtgg actatcoagg 11521 gatgtgggga gggaatatca ggaaagatct gaaacaaggtgtgagataac agatttgaac 11581 ttggaggcag agagtgtaat ggaccagggc cttctggactggcaacttct ggaattcttg 11641 ttaagcatag tgaggttggt cctatgaagg aggaagaatattttgggggt gagaaaactt 11701 gtggatgtaa gtctggtgct gtttttagtt tggaatggtggatttagtgt ggaaaggatg 11761 ttagttttgt tgccacagga gtagtcagga ttacctggtgaggacccgtc cacttaggtt 11821 ggagagggga gaaggaattg gtgatttaga cccagttccctggttgtagg ggcagggagg 11881 agtgtttgga ggatggactt ttaggctggg gcaagtaagcatttgcgtac cgtcttatta 11941 gatgttgagt gaggtataat gccagccaag tatcctgtatcaaRgaggtg gaggggcggg 12001 ctgggggcag atacagggag attttygagg ataaaagggcattggtacat gaattcaaat 12061 gggcttaggc tgaggggcgt gtgagcaatg gcttgtaaattcatgagtgc cattggaaga 12121 agtgaagttc aggccatttt aacctctagg aagtttggttatttgttgtt ttaaaaaggc 12181 attgacctat ttacttttat ctgaagattg ggggcagtaaggaacatgaa aggcccattt 12241 aatgtttaca gcctccacca actgttggtt aacctgtgaaacaaatgttg gcccactgtc 12301 taaccggatg gaggagggga gtccaaacca ggggatcatatgggtgagga gaatagaagt 12361 gatggtgtgt gccttttcgg tggtggtagg aaaatcttttatccatccag agaatgtatc 12421 tacaattgtc agaaggtacc aggcccattt tatggggaccatgagggtaa agttgatctg 12481 cccgccctgc cctggcaggt gtccttgggc ctggtgtgtggggaaaggag gtggtttgat 12541 agctccctga gaggaagttg tttgagtgca aatggaatatgccttagtaa tacctatgag 12601 attggcagcc atggcgggaa aatatatata agcttttaagagctagagta gggggcagta 12661 accggcatgg aaatggttgt gcacatatga aagtacagaaagttcctggg acttgggcaa 12721 gacaatttta ttattggggt agaaccatcc tcctccccaaatagcaccag cccgggtgag 12781 tgaggtttgt tcctcctggg tatatacagg gtgtatgctgggaaagatgg gcaataatga 12841 tgaggtttta agggctgcct gctgggctgc agagtttgctgagaagtttt ccttggttat 12901 ggcatctgta gccttttgat gtcccttaca atggataaaggtggccttta gtggtagttt 12961 agctgcctcc agcagtttgt gtatgagttt gccatttactatgggggttc cttttgtagt 13021 taggaaacct ctttcctgcc agattaaggc atgagaatgtaggatgtggt atgcatattt 13081 ggaatcggtg taaatgttaa ccctcttgcc tttactagggtgaaggccct ggttaagaca 13141 actagcgcca cctgttgaga ggtagtatgg agcgggacagtgctggattc taggagttta 13201 ttttcagcaa tgatggcata gccagctgct ggacatggctccctaaaaca gctaaaaaga 13261 tcttccatta atgaatcatg tgggtgttcc ctgcaaaggggcttttgaaa tgtgttggaa 13321 gggggagaag agggagttta agaggtccag gcaggagtgagagagcttag agtcagaggt 13381 gtttacaggg aagagggtgg ctaggttgag agctttacatttctagaagg tgattagagg 13441 gttacctatg aataaggcat gtatctatga ataaggcatcctgtaagcag gatggtggga 13501 gggatagaag agatcaatgg ctcatgagat cctgtaggttatgggaagat gcagtagtaa 13561 tgtgttggta gagagtaagt ttctgtgcct ctgaggcaagtaatgtggct gcacccaaga 13621 tttttgggca ggatggccag ccttggatga tagtccatttgttttaagag gtatgcaatg 13681 gcttgtggag cattgccata tgtttggcag agtagtccaagagcaaggcc ttggtcagaa 13741 tgtacacaca gtgaatgggg ccagcccctc cacacctgtgggtatttctc atcaggcagg 13801 aegagagact gagaaaagaa ataagacaca gagacaaaatatagagaaag aaaagtgggc 13861 ccaggggact ggggctcagc atacagagga cctgcaccggcccggtctct gaattccctc 13921 agtatttatt aattactatt ttcactatct cagcaagaggaatgcggcag gagaagaagg 13981 tgatagtggg gagaaggtca gcaagaaaac atgtgagcaaaggaatctgt gtcgcaaata 14041 agttcaaggg aaggtactat gcctggatgt gcacgtaggccagatttatg cttttctcca 14101 cccaaacatc tcagtggagc aaagagtaac agcagcattgctgccaacat gtctcgcctc 14161 ccgccacagg gcagcttttc tcctatctca gaattgaacaaatgtacaat cgggttttat 14221 accgagacat tcagttccca ggggcaggca ggagacagtggccttcctct atctcaactg 14281 caagaaggct tcctctttta ctaatcctcc tcagcacagacccttcacgg gtgtcagcct 14341 ggggggcggt caggtctttc ccatcccacg aggccatatctcaggatgtc tcagtgggga 14401 gaaaccttga acaatacctg gctttccagg gcagaggtccctgcggcttt ccgcagtgca 14461 ttgtgcccct ggtttatcaa gactggagaa tggcaatgacttttaccaag caacctgcct 14521 gtaaacattt tgttaacaag gcacatcttg cacagccctagatcccttaa accttgattc 14581 catacaacac atgtttttgt gagctcaagg ttggggcaaagttacagata aacagcattt 14641 cagggcaaag caattgttca gggtacaggt caaaatggagtttcttatgt cttccctttc 14701 tacacagaca cagtaacagt ctgatctctc tttcttttccctacaataca gagtagaagg 14761 cttggtggag ttgggcagtc ccagtactgg ggccattaaaaagggcattt ttcagctttt 14821 taaagtggga gttgatgggg caagctgggt tgaggggttttaggatgagc ccatgtgagg 14881 ccatatagaa cagcttggcc agcaagccaa agttgggaatccacagatgg aagcatccca 14941 tgcagcctga gaaggagagg aggttatttt tcgtgtggggaagaggcatg tcccaaatta 15001 gctcctttca ttgggttgga atggcctgag aattaggggttaggacaagc ccaaggtaag 15061 tgacctgcat ttgggctaac ctgagccttt gtgggtaagaccctaaaatt tacttggtat 15121 gtcccacccc aagatagggg tagggcactg agggataaacaggaaagagt gggtgaaggg 15181 ggtgatgaat aggttgcata atagaggacc agtctgtttgtgcctagagg ggattctatc 15241 gactcccaca atagagatag aagaactgag gaggggctcagaatattctg gtaaaattga 15301 ataactagcc tcatatttaa tgggaaagac atgggcttagcagacactga cagcattact 15361 cttggttcca aggtggtgat ggtggtggga gtggeggattctgggcccta tcagtcttca 15421 ggtagaggtg gtgaagtggg ataaagcgtt ttgctgagcacagtctgaga tgtgctccct 15481 gtcctccagg gtgagggagg aagagaggat gacgtaagtgtcatgccagg taatttataa 15541 gattgagtga catacagaaa ttccttgtgg aaggaggtgggatccatgga aaagaagcca 15601 agtctttttt caagctggaa gaggtcagct agggagaaggaaacataaac tcagattata 15661 ccttcggccc gcgtgacttc ccgcaagggg atgactttggaaggcctttg ggtgtatgcc 15721 tggttttcag aggaaggggg ttaagtgtga gactgggtatgcggtggaga agaaaggggg 15781 aacagagggc aacaataagg gaKtgaagga gccggagctgaaggaggagg aggggatggt 15841 ggtgggagaa ggactgaagg aggggttttg ttgtccgggttgaaattaga ggaggaaggc 15901 ttgtctgaag gaaggggcat ttttgggttt ggaggttccagattagacat ctgagagtgg 15961 tgtctggagg aagttttgag gagccttgcc ccatactggtggctggaagg gtgagaaata 16021 tagaggaggg gtgtgagtac cctggagggg agtaagtaccatgattcctc ttgggaggag 16081 ggacataaga aagccagggc atccctgcag ctcttcacagtcctctggag atcagagtga 16141 ctggagtggc gagcattctg agggcgtccc ctgagaaggcagaccatggt cacctggtga 16201 ccagggagac ctctcaccca gcactgggat tttctggtgaaggagcagaa gcatgaKagg 16261 aatctggaga ggaggtaaag ggagactcac ccattaattggagacggcgt tggacgtgat 16321 gttctgcaat gggggtgatc cttgccggag ccacccagaaagggggagaa agaaaaaaga 16381 ggctagagag tttgatcagg atctagaggt cagcccagggccaaaggaga gagaataaga 16441 gaaaaagggc tgcaaagagg gagtctactc aggatctggaagcaggcctc agtctaattt 16501 ttgctgcttg ctgctttcca ggttgcaaga gaagacttacccgtttagaa cccaatctat 16561 gtcctgggtt tctgcgccaa aatgtagtgg agtttttttcataatcagga gtttgagagg 16621 gtacaatgga agttgttagg ttttgaaggg aaggtgagggttaaagaaag agagttggat 16681 gctttgcagt aaatgcaggt tttatgtcta gcccaagacctgcggaggtg ggggaccagg 16741 ttaatgcctg aacccactgc cacttacagg ctggggtacttacaggtatg ggagggaggg 16801 gtctgggcag tatggcttgc tgcccaacag gctattgataagatgttccc atgatgaggt 16861 gacttggtcc ttgttccagc agaatgtgat agggatgttccttggacttt ttcccagtgg 16921 aatgtgatca gaaagtcagg tggtcaggcc gatgtttctcacacagagaa ccacttccac 16981 aagattaatt tccatactcc ttccaccact tgtttgaaccttcagcttta tcctattaaa 17041 ttcaaaacaa tcttttaact ctaggcaaca atttatatttccatggcttc ttataacctt 17101 ttagtaaaac acacacacac agacattttt ccaaacacaccttgcatgta aaaatatttt 17161 tagtagtatc aattacatgt tataatgatg actcttagcaatcttacttt taatgtaaaa 17221 gctggtaagt tatgttaatt atgtactagg tgctgataaggtcttactat tccagcatag 17281 ctaggggtgt ggccaactcc acatgtcccc aggccttacctagctgtaaa gcaggcaagt 17341 caaacaattt tcaaaagcca aagaagcagt ttatgaccttaaggcactta gcaaacctaa 17401 tatttgaaca taatttagat gacatgtttg tattttgaagacatttgtga aaactttaca 17461 gaggagataa acagtgactt tcacgtctca tttagccagtttgcacagag agaaagaggt 17521 cagagtctga ctggtaggaa attcttatcc ttttgccagtaggccaggtt tctgggtttt 17581 ctctccctga gcagccctag tgaMcctgct caactgtatgcaaacaaaca cactaccatg 17641 aattaagaac atttacaaat agtttacaaa ttttggagaaattaggcaga gagagaaata 17701 tgacttaaat tctgtttaca aaagtatact caacatacttaaaatatcaa taagcctaaa 17761 ctccaaaaag ttagtttaag gttaaaaagc tgatgtgttccattaactcc tgtgggtctg 17821 acaaaggtag cctaggaatt ccagataaat ggaacaaatgatgacttgct agaaatgcac 17881 aggaaacaaa ataactatcc acaaaaccaa ataaaagccttctagaagca aaaataaata 17941 aacaggaaag caaccctaat tttttcttac tcaatttaccctggaggcta aagtgttgcc 18001 cagggcccca aaagagccac ataataaata ttttattcctgatacacaat tcaatatcct 18061 taagttcacc aatatcatca tacatcctgt gcaatcaagaagttcacttt aggcccatga 18121 ccaataagca ctccagtgcc agcactatcc atgcaaaacagtaaacatag tgtgaagcag 18181 tgcaagcata tctgggaaat ttggctccac actaaatccagcttcaagct taactatata 18241 aaaaaaagaa ttgccaaact gccaatgcat ttctttacaatagttattat tttactttaa 18301 tcaagactaa gagctttaac tacgaaaatg ttaactagcccaatgtctcc aattctttat 18361 catgttttaa agaatatttt attatctaaa ctttttccacatccttctcc cctaatcact 18421 ggttctttac tacattgttt cacaaataat tttttcaaatgtgtaatttg aactaactbt 18481 tagataactt cttaattaga caaaattatt ctttttctcagtaataacac aaactttttg 18541 gcacattttg tatacagaac tctgtgttaa ctagaagtattatccttagt aacctaaaat 18601 tttagtgaaa ccctaaaaag aaatactgaa ctatcagatatgggcattta tagataagaa 18661 caattccaca atttttagaa acatatttcc ccatatcacaacccttcctt aattggaaat 18721 gacccaaata ttcaatgagc atcaaaatta attttaagcttttaatttac acaaaaagtt 18781 tacctaaaac atttatccca tttactgtac tcaattctttcatttttaac agtttatcta 18841 gattacttct gtaaactgag atattagaca ctatcacttaaagttagtta tttccttgct 18901 aaccatgttt ctaatagcca gtgaatatca agtgctcacctaaacctgag agtctcagaa 18961 ttaaatatat aagtattttg ccaataactc agaagacgtagctaacatta aattgctctt 19021 atttgtcaaa aaaaaaaggc acattttgtt ttggctgggtttgtagtttt ataacattct 19081 atgccaaaca ttgacatctc aaaatattta gcaaacacaaacataaaatc cagacaaaat 19141 gtatgctgac aattctgaag gcatttctat ttttattctaccaataattt taaagccagc 19201 ttgtttagta aagttatact taagtcactt taacttaaaaatcgcttagg cttattaatt 19261 tatgagcgct cttttactta taagccaatt tggcaaacataacatataag tgtacataca 19321 aataaacaca tctagacatg tatacacaca cacaaatgaagatctaatag cttgaaaccc 19381 tagccatgag atagcaacac aagcttaccg gttttactttgtttgcccca gtaggtaatg 19441 caatgaaggc tgtgaaccaa aattttgggt aaagcaatttccatggcagt ttgatttttt 19501 ttgtttcttt ttcttttttt tttttttctt tgagatggagtcttgctctg tagcccaggc 19561 tagagtgcag tggtgcaatc tcggctcact gaaacctctgcttcccaggt cctggttcaa 19621 gcaattctcc tgcctcagcc tcctaagtag ctgggattacaggcacgtgc caccatgtcc 19681 agctaatttt tgtattttta gtagagatgg ggtttcaccttgttggcctt gttcatgtct 19741 tgaactcctg acctcgtgat cYgcccgcct cggcctcccaaagtgcaggg attacaggcg 19801 tgagccaccg tgcccagatg gcagtttgat tttttttgtttttgtttttg agacagagtc 19861 tcactctgtt gcccaggctg gagtgcagtg gcgtgatctcagctcccact gcagtctccg 19921 cctcccgggt ctaagtgatt ctcctgactc agcctcctgagtagctggga ctataggcac 19981 gcaccaccac gcctggctaa ttttttgtat ttttagtagagatggagttt agtagagatg 20041 gggtttcacc acgttggcca ggatggtctt gatttcctgacctagtgatc cacctgcctc 20101 ggcctcccaa agtgctggga ttacaggcat gagccactgcacctggccct agcagtttga 20161 tttttaaagg tgaaacctcc ccagactcca aagaacactaagaccaaaca gtaccaaagg 20221 agaacatcac tctttcacca ggccctctgc ttagaatagcagcaccaaag cctggatata 20281 tgcaatggca atgccatccc agttttccat tcaacagtaaactccgcatt ccaaacaata 20341 ttggggccaa atagcattgc aactgcgaga taaaattctaaggagagttt aatactagac 20401 ctcagaacct ctgccaaggg catcctcttt ggagaggttgaggtccggag gattcccctc 20461 tggggcttcc cctagtgggg cccaatctta agagtgtcagacgtctctga ccttaggtgg 20521 gcaccagtcc tgcttgcatg ttttccctct aaagggaatggcctactatg agctttcatt 20581 ttgtccctgg atgaaggcct tgacttttag catccttataatttgataag gccacacttt 20641 cccatgtttc ccattccatg aacttaataa ctggaggctggatgtgtttc ttttgctctt 20701 agccagttga ctagggaaac ggaagaatta cgcataagaaaaggtttaag ttgcctgaaa 20761 caggcgagtt tgctctgagc tgtgccacac atagggatcagggaccacac ccagaaaaga 20821 tttaaaaaag agtccttctc ccttctgggc agggcaattatccccattca ctccatggtc 20881 ttcaggcaat attggggatt gatcccagcc aatttccctcaatttccaaa gagctactag 20941 gaaacagctg ctaaaagacc aataaagaag aaaaggaaaaaaaaaaagaa aaagacccag 21001 gtcccttagg caaaccaggt ggtggcggtt aggcttcttcacatgaaaac cccttagtat 21061 tcaccagcca tggccagaaa cctgcagttt acctccatgtttacacgctg ctcaccaagg 21121 gtcccgagtg ggaaaggaaa agagagagag agatttccctgtatgtaaaa agggaaagga 21181 gaaaaataaa tcccaaactt tgggcttacc ttctcctggctggctcacca aaatatgtta 21241 ccagtggagg gtgtccaggt gcttggcatt ttgaacaaagaattggacaa aatgcacaaa 21301 caaagcaagg aaagaatgaa gtaacaaaag cagagatttattgagtacac tccacagtgg 21361 gagcaggccc aagcagccac tcaagggccg ggatacaggatcttcttggg tccaaatacc 21421 cgctggaggt ttcccattgg ccacttggtg ttcacgccatgtaaataaag tggtggcccc 21481 caatcagtct gattgtttgc agacggaaac caatcagaggctgaagtgaa gttacaaagt 21541 tacactctta ggcaaacgcc tggttgccaa aagcaaccaatcagaggtat tttctttttc 21601 tttttctttt tttttctttt tgagacggag tctttctctgtcacccaggc tggcgtgcag 21661 tggcccgatc tcggctcact gcaagctcca cgtcccgggttcacgccatt ctcctgcctc 21721 agcctcccga gtagctggga gtacaggtgc ccgccaccacgcccggctat ttttttgtat 21781 ttttagtaga gacagggttt caccatgttg gccaggatggtctcgatctc ttgacctcgt 21841 gatccaccct cctcagcctc ccaaagtgct gggattacaggcgtgagcca ctgcgcctgg 21901 cccagaggta ctttcaatta accatctgcc atgcagaaaaggtgggagtt tgcaaagaga 21961 gtagcctctg gtccttttgt tacttaggca tggaaagttagggttttcct ttcaatgtag 22021 ttctacgaag tcagcgtgaa atggccttag gtttcctattctcctgcctc actggtatcc 22081 aagggactct gccattaggc tagctgtgga gggatggagagagagctgag gcctcaaatg 22141 ccagtattcc ttccttccag atttgcgtgt aagaccagataatcttccag accaaacagt 22201 atctgttgat gagtattagg ctcgaatgag aaggagagagaagtggagca ctggattgaa 22261 gagaaagcaa aaaaccttcc tagatcaaca aggaaatagcctcttctaga atccagatag 22321 ggccctggac tcctgtggga taatgtagtg ggtgaaaaaattggtaRtga agtcctcgga 22381 tatggggatg cacttggaag tcttgggaac aaggaagagagacatcctgt ggtggtgatt 22441 tttagatcca acacccaggg gcttgggtgc ctttggggggattagtagaa gagatcgaca 22501 cattgaggga ggttcaggat aactgtctga aaatgagagatttttattgc ttatactgat 22561 gtcccaatag ggtctgtatg tgcttcaaat aggtagaaaagaatgaagtc cgagcctgaa 22621 gataacaata tcagtcagaa ttttaggatt gtgaatacctgtccacagaa atagctgggc 22681 cctgagcctc tgctgatgat gtaggaatRg aggcagttaataagctttaa agtcataggg 22741 tctcagggct agggtatggt gaggacactt tgccacccctaaggttcaaa gcctcttgga 22801 ctggaggagg ttgttaacag atattctagg cagaaaagctgtgtcaggtt gtggtgtatg 22861 aagagggaga atttgacacc aaatggtcag ctggccatctgctcacctgt tcccctgtgc 22921 tccctactcc cacagggtct gcttacagaa caaggcacagcaaggaggca agacacaagc 22981 aagaaatgtg cttgatgtta cccactgcag tccttcttccactctggagg attctgaaga 23041 ctcagaagag ggacaggcaa tggattcagt ctaaaccccaggaccaggac actgcctcag 23101 cttcgaagcc tggatgcaat gctccagggt ataaccactgcccagaagca cagctagtac 23161 aagctgcagc ctcaatccag gcaaggagcc aactccaacaggtcaggctc caagggatga 23221 agactgaaaa catgaatgtg ttgtgccact aactctagcgtttgcttttc cttgtgtttt 23281 tgcatgcatt gtagaccttg gcatcagcat ttggctagctttaagacaga aactgagaag 23341 ccttttgtgt tttatgcgtc aacttgactg ggtcacagcatgcccaaata tttggttaaa 23401 cacgatttct aggtgcatct gtgaggattt tctggacgagactaatattt gaatttgaat 23461 cagtggacta agtaaggcag actgccctcc ccagtgtgggtggccctcat ccttctctct 23521 gcctgacact cttctagctg gaacatttgt cttctcctaccctcggactc agactatgac 23581 tggaattgat atcattgact ctcctagttc ttcagactcagattgctctc Scgggacttc 23641 tcagcttcca aatcacatga accaattcat tataggaaattttttWatat atcctattaa 23701 ttctgtttct ctggagaacc ctgattaata cagatttattttcctacact ccagtaactc 23761 tccactactc cctccctcaa cccctggtaa ctatcctactttctgtctct ataaatttga 23821 ctattctcag tacctcatat aagaggaatc atgcagtatttgtccttttg tgactggctt 23881 atttcattag cataatgttt tcaaggttta ttcatgttgtagcatgtatc caaatttaat 23941 tcctttttga ggctgaatga tactccattg tatgtatataccatattttg ttcattcatc 24001 cattgataaa catgttgttc ccacatcttg gctattaggaataatgttgc tgtgaacatg 24061 agtgtacaaa tatctgagtc cttgcctttt ttttttttttttggacacag agtctctctc 24121 tgttgcccag actggagtaa agtggtgtga ttctcggctcacggcaacct ctgcctcctg 24181 ggttcaagtg attctcgtgc ctcagcctcc ccagtagctgggattacagg cgtgtgccac 24241 catgcctggc taattttttg tatttttaat agagatgggattcgtcatgt tgcctaggct 24301 ggtctcaaac tcctgagctc aggcaatttg cccacctcggcctcctaagg tgctaggatt 24361 acaggcgtga gccaccatgc ctggatcatt gcttttcattattttgggta tatacctaga 24421 agtgggattg ctggatcata tggtcattct gattttttgaggaaccatca tactgttctc 24481 cacagtgtct gatgacctac agttttgagc ttagcctttctaaaatagtg gggaaaaaaa 24541 tgaaaagaaa acaactagaa gtggaagtaa tttgtccttcattaagccag atatacacat 24601 cctgattcaa gcttgaaaac aatatcacct ccttcgctgatatgtaagta cctttttata 24661 atttaaattt taagttatga ttaattagaa acttaaaatgtatagtctgt atatgcactg 24721 gatgtccaca aagtgaccct tggttgctaa atctttgagaaaggctgagt agacactgaa 24781 cgaatgtata tccaacttac ttgtcaactc ctccaagaacattccttacc ccctgaccca 24841 agacatgctt tctatgagct ccaacagtgc ccctagtcttcaacagctac cacatctgtg 24901 ctcgtcacac tcttgtcctt ctcctagtct caactgtgagccatggatgg cagaggccgg 24961 tttttaccct tcaggtctcc agttaggcct gtgatggttctagggttttg tttaggctgc 25021 gagtctgatt ggggctagga caaagggtca aattcaggatgaaaatcagc ctggccaaga 25081 gcagtggata catagccccc atcatcccac ttctcagatgctcctagaac tccagcccgg 25141 gatcctttat cctctgtata ctccaccttc tccccatacaagaccctctg cagagagcag 25201 gcacaaggag cagataaatg atggtcgatg ctgcctgtcaattcagatgg gcctggcata 25261 acggttaact aatgggtttc ttgtttgatt gataggttctctcaaaacta aaggagttaa 25321 atttctttaa tagcaataca tgctcattgt taaaaactcatatcaactga atttcccttg 25381 aacactatta acatttagtg tattatcctt tcacatttgtatagatattt agaaaaatgg 25441 agtgtagtat atacgtttat atatttttgc ttagcagtacatcatagaca tctatttcat 25501 tatattcaaa tggctcaata atattcccct gtgtagctgtaccataatct agccaaactc 25561 ctatgtgggg gcctggtttg tatttgattt ttcagtaaYataaacaatgc tgagataaac 25621 ctctatcagc tagattattt ctattgactg attttaatacatgattggaa ttttaaccac 25681 aagggggaag caggcaacca tgggaagaca ggctaagatttaggataaag aaaaagaggc 25741 tgggtgcagt ggctcatgcc tgtaatccca gcgctttgggagggtgaggc aggaggacag 25801 ctcaagccta ggagtttgag gttacacaaa gctataatcacactactgca ctccagcctg 25861 ggtgacagag caaaaccctc tctctcaaaa aaaaaaaaaaaaaaaaaaaa cgatttagaa 25921 agttatctca catacataag cttaccccaa ccttgtgagcaggtagtagc attagtatta 25981 gcctcatttt ttggaggcaa agctgatgat ttcattatcaggcgctcttc tgtatttcca 26041 aataaattga cagcagtaca acattctgcc tacattcagttcaagtccag gtgctgtgct 26101 ggggacacta tgaggcatgc agaaataaag aagacaatccctgcccctca gggggtcatt 26161 atcaggtgtg aggacagaca cacctaaata accaagtgcccactgtaaca gacaggagag 26221 cccaggaagg taggaaattc acttcgatag tgaggctgggcacagtttta tggaaggggt 26281 atgctgacaa taagaggtaa ggtgtattta gcctttactctgtgcatggc actgtgctaa 26341 gtgctttacc tgcataatct cattaaaact tccccacaggccaggcaccg tggtttatgc 26401 ctgtaatccc agcactttgg gaggccaaaa caagaggattgcttaagccc aggagtttgc 26461 aaccagcctg ggcaacatag cagggccctg tctcttaaaaacaaaccaac caaccaacaa 26521 acaaaccaac aaacaaatcc ccatagacct ggaaggtatcctaccctttt ttacagagaa 26581 gaaaactgat ggtcagagag gttaagaaac cagctaccgtggcacaacac tggaacgcgc 26641 gtccttaatg tgaattcagg aggccctaca agggttggtagactttcaac aggcagggct 26701 gggagagaga cttctaagag aggaaacaca gaaacgaagccttagaggca agaaatacag 26761 gcacgtctgg gaatggcaag gggatcagcg tgcctggggtgtgaaaaagg aggaatctga 26821 agagatcaaa actagagaaa gaaaactgag tcagagtggtgaagactgtg agtagcaagg 26881 caaggagatc ccgctgtatt ttgtattcag gtgaaaccaactgaagataa tgacatcatg 26941 tgtgctttat gaggattaat ttggtgcatt gcMtaagagaacaaggagac tagttagggg 27001 cttgttaaaa tagtcctggc caaagataac tagcgaaggaatgagaacca gaagacatta 27061 cagagataaa atccacaaga tcttattaga tgctggggaaagaggaaaga attgtgatta 27121 agattttcat gtttgggcaa ccaggagaat gtgagtgtgtacaccaggag aggggctgga 27181 cagaagggag aggcgagttc acttttcttt tatgagctggaaggaccaca gggacctttg 27241 agtggagatg tttagaggag agttagaaac aggaagctggtgatttagac atagtacctg 27301 agaatcagaa gaaacccagg gccgggtgtg gtgtctcacgcctgtaatcc tagcactttg 27361 ggaggccaag atgggagggc tgcttgaggc caggagttcaagaccagcct ggtcaacata 27421 gtaagaccct ctctatatag aataaaataa aatttaaaaaaagaagaagg ctgggtgcag 27481 tggctcacac ctgtaatccc agcactttag gaggccgaggcggtggatca cctgaggtca 27541 ggagttcaag accagcctga tcaacgtggt gaaaccccgtctctactaaa aatacaaaaa 27601 ttagctgggt gtagtggcag gtgtctgtaa tcccacctactcaggaggct gaggcaggag 27661 aatcacttga acccaagaga cagtgagcca agattgcgccattgcattcc aacctgggtg 27721 acagaacgag actctgtctc aaaaaaataa aaaataaataaaaagaagaa gaaatgacca 27781 agggagctgg ccagggaaaa acctacactt aagattcagcaaggcatgtt agaccctgat 27841 aacctgcaat tccacagcca ggtttctatc ccagagaaacaaaatcacac gtgcacaaga 27901 gagcgtatga aagttcacag cagcattgtc tgtcttggtgaaagcctggg tcagggagga 27961 gcaggcaaat aaaccattaa acatccacat tgtgcaatagttaccaatta agtagatcaa 28021 aggtattgac atggacagat cttcagaaca tacagtggatgagaaaaaca acatgtagtg 28081 caataataat gtataaaaca ttacttatga aaaaagaagaaaagccaaac aatcctgtca 28141 catatatatt ttaaatgatg tatgtgtagg catagagaaaattctggaaa gctaaatcca 28201 tgctaattgt gataaagggg aggagttggg acaaacatgaagagcagaag gggttaaatg 28261 gggttttagc atagttgtca ggttttaatt ttataagaaaaatgtaatca tctgttactt 28321 atgtgaataa aaattctcaa aatattttta aaaagaaagattcaatggga agaggaagca 28381 atggaataga caggaataca ttcctagact ctaggtaggacactgttact gggaacactg 28441 ggagaagttt ctggaaKgca aggtggaaaa aggatccactgcactcagca acgctgcaga 28501 gacagcagcc agcaggatga gtcctgtgag ctcagctggttgtcaacacc tttcacttac 28561 agccactagc tcctgtgtcc ttgaaaagtc gccatgtcccaccactgccc ttgaatgaga 28621 tggagacttt ctgttctttt ttgagttgta aacatccaaaagcatttcRt caatttcttg 28681 tatttagaca ttcccttcct ttcccagaaa ttccacagtgatattctcag tgttctcttt 28741 ccagtccttc aagctgtgta tgaaatctac ttcttcccagtccagctcca gcaaatacct 28801 gcctcctctc ttgttctcca ttcaaaaaac ttccaatttgaggtcatttt taagagcatt 28861 actgtggtgg tggcggtggt ggagtttata gaatttctcatgggacccac agcatggatg 28921 tggctaacac tgactgagca cacaaaggtg caggcactgtgctaacttca taggaagtat 28981 ctcggttaat cctttcagca accccagtag gtcagtactattataacctc caaggtcttt 29041 ctttcacagc tgggacttag tgtggctgag cgacctaaggaggtaagtgg cagtaagctt 29101 gtggtgggtg actctgtgtt tgtctcacta tgtgagtttgtcctaagtga tcaatagggg 29161 tggggactgg actagaaaca aggtctcagg tatgcaggggtgttcagttt accaaatgcc 29221 tttatgacca ttaattcatt tgatgtcacc acagtgctttaatgacatgg gtcattaggg 29281 aaaataatga gatgagtcaa gctttgtaat tcccttaaagtggtcaagtc tgggttaagg 29341 gcttttaggg cacattaact cactatgtcg tcttgagcaactctgcaagg ggtgggagca 29401 gagggggccc cctttggccc agctctctac aaatcatctagttcctagca gccactcctc 29461 ctggtctgca aattcttggc gaggcaccct cctgctctccaaacccaccc attccaggcg 29521 atcccttaag tggcagagga gccaggttcc tggggttaataagcaggctg tttatagcca 29581 gcagcagtca cgggacagcc agctggaagg tcatgccccttctgaatgtt gaggtttctc 29641 ataaacattt ccccagtgag ccttctggga gatgaggatgactctgggcc cattgccctt 29701 gctggggcag agcgccaagg ccccatactc actttcttctcctttctccc gaatctggct 29761 ctcttcctct ttcctccttc ctctcctact ctgctgtcattccttccctt tcttcagtgg 29821 aaagaggaca cgtttcacag tagacacaca ggataactcaaccttctgag cctcagtttc 29881 ctcctgcgta aatggggtaa taatgggRcc ttcatctcaggatgtaaggg aaagtgctta 29941 gcactgtgtt aggcacgcac agtggacatt taaaacatattggtcatgtc tttccccctc 30001 caccctatcc ttttgcccta atcctggcca ctgccctgttttctttctat tttcctgctc 30061 cagccctgct cccatttcca ccctgggcac ttccaccccactgaYtgtgc tgctgctgcc 30121 tgggagggta gacgaagaag ccaggcttca ttcagaggagggctgaccac cgcagggccc 30181 gcctggcctt ggcacttccc catgagaccg caccctaggcctctctgctt ttagtaaata 30241 gcccatggga acagggggtg gagggaggat gtagaatgggagaggccaag acattttaac 30301 ctttggcttc tatttgccct taatctggct cttccaatggcttaatgatc agagatcatt 30361 gtgtaaattg ctgtgtgact ttgggcaagt ccccttcactctctgagctt cagtgaagtg 30421 tgggaaggaa gtgaagagca gaagttgggt agaagtgcaggtcctggaac cagaccatct 30481 ccaccacttg ctagcttgga gtacataact cctctgtgcctcagettcct caactataaa 30541 atgcaattta tagtatacct aatttataaa agcacttttaaaagtgctta gaatagcgcc 30601 tagcccatag ttggtactaa attaatctca gctattatatgcaaatggag aggtagaaca 30661 ggggtatgtg tgcatgagag gagagctgga ccttctctgagggtattcta Ragtcctgaa 30721 aacccacatt cctccttttt ggataagaag aagacagaaccttgaacaca aagtacttct 30781 cccaaggaga ccataggaaa cttgtcaaag acttttaaagccagatggac cccttagagg 30841 tcatctggtc Yaacttgtca tgctacagta agaacagggtggaccagaga gattcggact 30901 tatctgggac cacagagctt ttgacgagtt atagaattgcaggtggtgca ttgcagggaa 30961 gggaggcact ttaagaggac agtgggatgg gctgccagagggctggtggg atgctagagg 31021 tcagattccc aggctccatg acctccagga ttcttagttcaggcacttac cccataacta 31081 aagcactgat gtcaccaccg gcatctttaa taattataaccatgtaatga ggagggacta 31141 tatcccaagc actgcaccag acgctttcac atgcattacttactccatac aaaatcccct 31201 tgRcaaatga ggaaacacgc cttgcccaag ctgcaacaattacaaagggg cagagctggg 31261 ctttgaatcc cggtctctct gatgccaaaa ccagggttctcatgcaacac tcttttcatg 31321 tttcccagag cctagtttga ggcttgagcc tcaataaataaggatcttgt atttgtctca 31381 ctgggctggc caagcagatc ttgtatttgt ctgactgggctggccaagcg ataacactaa 31441 tgaatMagcc tgggtcccag tcacctttcc agccctttccaccctgaccc acaagccatg 31501 atgagagacc agaccctgtg taggaaatct ttcttccaggggccctgcct ctgtgttatc 31561 tgeccccact gtcaatggga gaagcagtgg ctgctttcggaacaggcagg ggaggagggc 31621 ttagagcctg gggtgacRct tctggctgct gggcctgacctccccagact ttagccaggg 31681 ccctgacttg aatgggagga gcatgaacaa acagaaagttgtaggagaag caagaaacca 31741 ggtaaggtga aaaaaacttt tcccctccaa aaatacgaagtttatagttg atgtgaattt 31801 gtcaacttaa tatagtgtaa gaagcataag gtttaatacggtcttcctcg ccatgatggc 31861 aggaagcaca tctgttgtat tcactaccac atgcttagtgccccacccaa ggcctgtcta 31921 gagtcagtgc tcaataaata aacatggaaa atgaatgaaaaaaaaaaatg gagggacata 31981 aggaagagca ctgaaacatc agtcagaaaa cctgcattacagtttttagc ttcatggctc 32041 acagcaaccc ttggacaagt cacttcggct ctgtaaacctcagtttctac aagtgtaaaa 32101 tagatatcat ggcagttcct actgcacagg gctttgaaatcacagacttc cgtaggagtc 32161 atggttccat aaccaactaa tgtcaagttg aaaattgtatttcttcagag tgaggccggc 32221 tttagcaatc tatgcaaggg gtcttgctga accagggccagaggaaggcc agaggtgtgg 32281 ttctaagaat gaggattaaa catgagagag actataaatctgcttggaga accgcagcat 32341 taggagggta cagcggacct ggcaggcatg acagatactctctgccctgg agagtctcca 32401 gccctgccca tgcaaggtgt cagttagtat cagcgggagcacagatgggc ttaaatcaga 32461 agatgttaca gattatctag tccacactgg acaatagcccagtgaggggt taaaggctga 32521 gagacaaaga cccagatatt ctaattccca gctcaatgctactcttccca caccaccatc 32581 aaaggagggg gacggtggtg agagggggta tttgaagagaaacagattgg acaaaggaat 32641 gaatgcctcc atgtagcaac agcttcaaag gggcttcagaataggagata tcttgttaaa 32701 gaaggaggaa cgagaggggg aaatggttaa aaataacgtcttacatccat gtggattatg 32761 tttccaacta tatcatgtgt agttattgca tgtaaggtactatggtggag agttactgat 32821 ggggcatcag aaagcccatt tcattaccca gctctgccatgtacaagctc tgcgacctcg 32881 ggcaaatcct ctctctgatc ctcaaatttt catgtgaagcatgaagcaat agtgcctacc 32941 ccatagacct atcctgggga ccaggttagg aaagggaagtggagggaggt tgtatcctct 33001 ctaaaatgct ctgcaaatgg gcaacatggc aaagcggtcacaagcaagcg tgtgaggtag 33061 accRtcactt agcctttctt ttcttttttt tttttaagacagagtttcgc tcttgttgcc 33121 caggctagag tgcaatggca cgatctcggc tcactgcaacctccacctcc taggttcaag 33181 tgattttcct gcctcagcct cctgagtagc tgggattacaggcgcacgcc accacacccg 33241 actaattttt gtatttttag tagagatggg gtttcaccatgttggccagg ctggtctcga 33301 actcctgaca tcaggtgatc cgcctgcctt ggccttccaaagtgctggga ttacaggcgt 33361 gagccaccac gcccagcccc acttagcctt tctacgactttgtatcctca tctgtaaaat 33421 ggatgtggtg ggaatcaaat ataatgcatt tgcagcctttggcacagtcc ctgacacata 33481 caaagcctgc aacatcctgt tagttactac cactagttgggtttaaagaa ttttctcatg 33541 taatcctcac aataatccta tgaggtagga ttattattattctcatattg cagatgggga 33601 aaccaagacc taaaaagaat tgcttaccca aatcaatagctaattagtag acagcaaagc 33661 caactagaac ccagatgccc tatcagatca ggcaggcaaagatcagaatt agtgagtagg 33721 gcaggcagca gcgggaatgt ctgtggagtc cagacaataatgcagccaca aagccagagt 33781 tctggtagct gcagaggcat ttccaacttt aaactctggtcagtctggag ctcagtcaga 33841 acaaaccttg tttcatcttt gatttaagga gctgagaaaaataagctctg gaaagagaag 33901 aggggaccct gtgttccagc atcatctgcg ttatttattcactaggctga ttgcttcKcc 33961 tctctgggcc tcagtttccc cagctgtata tgaaaggttgaactagatta atgcttctca 34021 aacttgaatg aggttctgag tcacctgatg atcttgtttgaatgtagatt ctgattctca 34081 gtaggtctgg gaaaaagcct gagattctga atgtctgacgagctcccagg ccatgccagt 34141 gctgcagagc taaggatcac tctctgagga gcaaggaactagatcagctt gaaggacatt 34201 tctggtatga taatgcctat gagagcaata caccaaagactgagaagtcg agcaaaaaca 34261 aattctgccc aaagtgcctt acagtatttg ggggtccagtgtgagggcca ggctatttcc 34321 catggaccac agaccccttt aaaaaggggc ttgatatccagcactcagtg aggcattcag 34381 ctgcagggcc agctctccag actcttcttc ccagcccatctcccaccctc acctgcactt 34441 cacctcctaa agggaagaga caaaattttc taagccatttccctccccta tcagggctgg 34501 ggagggagcg gcttgttcct aatgaagaca aaggacaaggcttaagggac acttgagaat 34561 ctttttctgt ccttcctttt cctcattcat tcattcattcttcattattc aactatttat 34621 tgagtactct ctacgtgcct ggtattctcc taggggctgaggttacagca gagaaaataa 34681 gacagatgtg gcctctgacc tcatgttgct tacattctaattgggaggaa aatgggacag 34741 gccataaata ggtagatttt aaaaatgaat gagatcatctaaaatcacaa aaagcaataa 34801 aagaataatg aaacaaagtg atggggtagg gggcttcttctgtctattgt cctcaagaca 34861 aagtccaagg ttggaaaagc tcatctccac ctgcccttgtttgcttctcc agcctcatct 34921 cctgtcactt tttgccttga actttccatt ccagccacacaggacatgga gtatcaaggc 34981 tggaagacgc caaccagcct ttgatccagt ggttctctcagccccaatca ctctttctct 35041 ttgtccctac ctcttcctct ttttccctct ttctcacccaactaacccct cctgctcttt 35101 cccagcttac tcgccactcc cactggaagc tggccctgctgccactgatc tgggctagga 35161 atccgtctgc gccaccttcc cWgatgacag catccatcacctgtgcagcc gtgttaattg 35221 gctgtccttc ccccaccaga ctgtaagtgc ctcaagggcaggagtcctgt cttattcact 35281 ctgaatcctg aatcctcagc agttgctgca atgcaaagcacagagtaggt gcttaaggaa 35341 cacctgttga attaactgac actaagttta gtatatttaagagctcagta atgggctggg 35401 cgcagtggct cacgcctgta atcccagcac tttgggaggccaaggtgggc agatcacctg 35461 aggtcaggag ttcaagacca gcctggctaa catggtgaaagcccgtctct attaaaaata 35521 caaaaaatta gctgggtgtg gtggcaggtg cctgtaattccagctactcg ggaggctgag 35581 gcaggagaaa cgcttgaacc caggaggcgg aggttgcagtgagctgagat agcaccactg 35641 cactccagcc tgggcgacaa gagcaaaact ctgtctcaaaaaaaaaaaaa aaaaaaaaag 35701 ctcagtaaca tgagacaaag gcatctacaa gacatggctttggagacaaa gaataaattg 35761 ataaattgga aggaaaagaa tataacaatt cctcagtcctttctggagtc cacattctag 35821 tggctttcag ctctacactg tatcaggcct cctacaaggcttaccaggca gactgggcag 35881 aaattattga cccccattca acagctgggg aaggtcatgcctcagagagg tgaacctact 35941 tcagctgaag ttacccagcc aggtggtggc aaagcatgtccaaagaaagt tagggaccag 36001 agacgtcaga tccaacccct ccctcttgca ggggatggggtaggggtggt aatctgcaga 36061 ctctgaacta agtgttctag ctcatttcaa actcagaataaccatttgag gtaaacagta 36121 gccatagcca caaaaaaagt agacacatga tttgcccatcatccaccttc cccctctaac 36181 ccaattgtcc ctgggcagac cgtgcagagt accaccccattcgtcctcaa ggactccctg 36241 cctggggtca actcatgaga aactgtccac ccgcagattgactgggacag aaaaaaccct 36301 agacttttaa tttccttatt tcacagttgg agacaatccaaagcttcgag agaaggaact 36361 agtctatata gcacatactg agacagggtc agaactccggagtcagaact ttctgaagta 36421 tagcccacaa gtccccttga catgaacctt ttgagttgggaaagggctaa gaatgatgtg 36481 aacatgacct ggccatttgt tagctgtggt caggctgggcgaagagaggc agcccctctg 36541 gtagttctac tctagaactc attagtcccg ccagtcctcaatggtttgtg cattggtagg 36601 tagcccatgg gaagtaggat gtgggttgct tactgcttagtgactgttta tgaagattgt 36661 tattgctact acagcagtat agtctcccag ctgaacaataacctagaaag tatggagagt 36721 ggccgggcgc agtggctcac ccttgtaatc ccagcactttgggaggccga ggcaggcgaa 36781 tcacgaagtc aggagttcga gaccagcctg gccaacatggtgaaaccccg tctctactaa 36841 aaatacaaaa aattagctgg gcatggtggt ggtgcctataatcccagcta ctcaaagggc 36901 tgaggcagga gaatcgcttg aacctgggat gcggaggttgcggtgagctg agatcatgcc 36961 attgcattcc agcctgggag acagtgcgag acttcgtcgcaaaaaaaaaa aaaagaaaga 37021 aagtatggag agtaggtgga gaggggctag cagagacataggtagggcac cttaccacta 37081 agaaatagag caaagggtag gtgctttatg ttccagaagcatatagatcc aagaggaggg 37141 aagggacatt ttggctgaaa gcttggaact atatctagcatagataaatg tgattctcat 37201 aggtgcttgt atttgctatg ggaccctgga ccaatcattcagtctcttga agccttagcc 37261 tctcccataa gagggtggaa tgagctagtc tctagggattcttcttttta taactctccc 37321 tctaagtggg ctttgcaggc agagggagtc attaactcctgctatggtct gagtgttccc 37381 cctcccgcat attccgatat tgactttttt tttttaagagtctcactctg tcttccaggc 37441 tggagtgcag tggcacgatc tcagctcact gcgacctccaactccctggt tcaagcagtt 37501 ctcctgcctc agcctcctga gtagctggga ctacaggcgcacaccaccat gcctggctaa 37561 tttttgtatt tttagtagag atggggtttc accatgttggtcaggctggt ctggaactcc 37621 tgacctcatg atccgcctgc ctcggcctac taaagtgctgggaatacagg cgtgagccac 37681 actgcgcctg gctatattga catcttaacc cccaagtgatgctattagga ggtgggaact 37741 tgggaggtga ttaggtcata aaggtggatg gagcactcatgaatggaata aatgccctta 37801 taaaaaaggc ccaagaaagg cccctcaccc catctaccatgtgagggtac agtgagaaga 37861 cagccatgta tgaggaagca ggtcctcacc tgatcccaaatccttgatct tggacttccc 37921 agcctgagaa ctgtaagaaa taaatctgtt atttacatgccacccagttt atagaatttt 37981 attatagcag cccaaacgga ttaagacaac tccatttcaggtaagcccaa caaacctagt 38041 atttgccagg ccaggtcctg ttgaggctta attgagaaatgtgtagcaaa ttgttagtag 38101 tacaggcccg agaccatgat ccttagaccg ctacacttggcctgtggtca gctcttggga 38161 acttctattt cagaaggatt tccaccattc cccaaactaataagagtagc tcattatgcc 38221 taaactggac aaacaatgca gtttctgttg actgcctaattttcttctgg gagtttagaa 38281 ttctggtatg tgcttagcca aggatgccta tgtgaccagcccctaacaaa aNcctggcat 38341 tgagtctcca gtgaacttcc ctggtaaaca acattttacacatgctgtta cagttcattg 38401 acaaaggaat gaagcacatc ctgtgtgctt aattcagctgggggatgatt ctgggaatct 38461 cgtggctgtt ttcttccaga ctttgtccca tgcaccttttccctttgctg attttgtaac 38521 cttgtgctgt aataaatcat agtgtcagtg tacttatatgctgagttctg tgagtcctcc 38581 tagcaaatca ctgaacctag aggtgatctg gggacccccaacaggagtta agcctagatt 38641 tcacagagtc aatcataatc ttgtgccagt cccttaagagtatacagctg aaaccagtgc 38701 tatccaacag agatataatg tgagtcacat gtaattttacattttctagt ggctatattt 38761 taaaaaccaa aaagaaacag gcaaaactaa ttttaagcacatgttttctt caacccaata 38821 tattattatt cttttatttt tattttcgag acagagtctcactctgtcac ccaggctgga 38881 gtgcagtggt acaatcacca ctcactgcag cctcaatctcctgggctcca gtgatttgcc 38941 cagctaattt tgaaaatttt tgtagagatg tggtctcactatgttgccca ggctggtctc 39001 aaactcctgg gctcaagcca tcctcttacc tcagcctcccaaagcgctgg gattacaggc 39061 gtgagccatt atgctcagct aacccactat attaaaaatatatttaaaat atttccatat 39121 gcaattttat taaattaaga tattttatat tcttttatcacactaagtct tcagaaaaca 39181 ctgtgtgttt tatactcaca gcacagatca gtttgtactagccctactcc aagcgtgcaa 39241 cagccacaca cgcccatggc ctgcctgcta tattggcttagaggttaatg gcaagggctc 39301 tggagacagt atatgcggag gctaaagaca ctctgtctaggatgctgatt caaccatgtt 39361 gatttctgat taactccagt tccaggaaag ccgctaaggttttcagttta tctactcttc 39421 cttgtgtaag agcatgtcct taacataaat attccccttaggtgaaaaca aacttaatat 39481 tctcacactt cagttggcct acacatccct tcaatagggaaatcgcccct tccctatgct 39541 atataagccc tgggctgagg aggggtaata gcttggggatccactatctc acttcacagc 39601 cacctaagac ttctgtggct tctgtttcta agtccctaataaatgtttct ttctgagaaa 39661 ctgggtatgt cagcttcttt cttcagcttc tcagcctcctctgactggct ggcgcggggg 39721 tggggtaggt ttacagagat ctgctcacag cagaatagtatgtgtgggtc agaatcccgg 39781 ttctgtgact taccaactgc ataaccttaa gcaaagttgcttaacctctc tatgtattag 39841 tttcttcatt tacaaaatgg ggataataat gttggctacctcaagaggtt gcagagatta 39901 attaatctaa ggcagcacct ggtatagata ggatattgagtgaatgctgg ctgctactct 39961 ctttatgcta aagcttggcc catggagatc aacttgtctaacacctcgtt ccataaatga 40021 gaaaactgca gcccagagtt tggaaggcac tagtttcacaagcaaaaact acagccaccc 40081 acctctcctc ttgatgaact ccctgtcttc tggctggccctgagatcaaa tttgccaatt 40141 atttaggaaa ttggcagctc cagggcccca gctcggttctgcaatttctc tcgattgcaa 40201 aacaagggct actgtttgaa gttggcactg aagtgacgcaggctgggcct cacttctgct 40261 tccacctagg ctcatcagtt agagtggctg atcccatctttgagttcaga acctgaaatg 40321 tttactaacc aataataagg ctggctttcg ttgttttaactttttacaat cctttgagtt 40381 tattaatttg tgttctatct tccttgaaga cacagtgcatcagcactttt tgtaaaacct 40441 ctttttaagt catcttcggt ctctgttcac ctaccaagtaggtaagtagt gagctcccta 40501 tagaggtctc taaaaaatag gatgaagagg ttccgagtgctcacttgtct ggaatgcgga 40561 agacgtgttc tcagactgga agcaaaacta gcttatgattcttctacagc ttctccttcc 40621 atccctccct tcccattttg gccaatgcaa atcatctactctcctctgac aagcctactc 40681 atcataaccc gcacgtcttg aatcttccta gaagatgaaatcagaccagt cttgcgggct 40741 ctacatggtt acaacttgct aacttgaagc tctcattattctatatgcca aatattgttt 40801 tccttgacat taatttattt gatagatatg tatcaagcaactactatgta ttaagctctg 40861 tgctggtgct ggtgYctttt tggatcttat aatttagtgcagaaaataga caaacaagaa 40921 atgatgataa agtacaacaa agggtcgatg tacaaagtgtcaaatactct aggaactaca 40981 ggaaaacaag ggaaccatca cgaaacccct gtcttaagacgtgacattag tggagatatg 41041 aaaaatgagt aggatagtac cagttaaagg gagacaacctctgttccaag cacagggaac 41101 agcagagatg aaacctggaa gtgagggaga atggcagactgcaagaacgg aaaaatgtcc 41161 agtgtagcgg aggcagagag aatatggaga gtgtggcaaaagaggaagaa gcagatggat 41221 tttgagggca ctttctgaag tgctaaggaa tttagactttaacctgaggg caatcagaag 41281 cagcctggtg gagaacgata tatttcaaaa catagggtccttgaaaacaa gtcaaccaaa 41341 tgtatttaaa aggctgctga tgaaaagatt gggaagtattgttttcattc tcattgtttt 41401 cactgtcatg ctctttgtgc caatggttag acctcctttgaaagctcact gcagcatgaa 41461 agcccttctt aatgccaggg caggctcccc aagcctccccaccccactgt ccatgtcatc 41521 agttttgtac tcctgttttg tatggctcag gctttccttatactcttagg aatgaaaagc 41581 cacagacacc accactgaga gactgtcaaa gatccttgttaaaYaggtgg acaactgaga 41641 tcagagaagg aaagtaattt tattactttc Ygtagcaatccaggggtagc atctcaggtc 41701 tctggctcct tcttgtctcc cctaaagaaa tagaccaccagggctgggat taaggtgaga 41761 caagtgagac atagagggtg caaaatgtag agagatgcccgctgccaggt gccagcctgc 41821 acttRtgtga ccctaggtgc cttcccttcc ctcaccctgctggtatctgc tggaaacacc 41881 tttgagagca gggactttgg cttttagaca actctttagcatctctcatg gtgttaacat 41941 gttgctaggt gcctattttg agggtcacta gatgcctactgatactaatg aacaatggat 42001 ttctgacaaa ctgatgtgca cacagatgtt tatattttagggtaaatacg aaccttaaaa 42061 gacaacagaa caacatggag agggcctgag actgagtctagagacctgga ttctggttcc 42121 ctccctgcca tcagttggtt atgggaactt ggataaattgctcctcatct ctaggcttca 42181 gggaaatgag gtggttgaat aaaacgtatt gagcacttactgtgtgtcag ccattgttca 42241 tatgctccca ggctttaact cagctaaaga cttcctggcctctgtggttc cttccacttc 42301 aacaggctgt ggtttgaggt gggaagactt cgtagagaatgcaaaacttg agagacatct 42361 tccaaattcc cagggggatg ttaatacctt taatcatagtaaaggtatgg tttctcaagg 42421 catttcttgg tcagaagaaa gttccagaag gcacttaatcctaggactca cactaccaag 42481 gctacagcca ggccaactgc ctcacaataa ccttagaacatgtctgaaga cagattttca 42541 gcttctgccc ctagagattc taatgcagtg attccaaagtggggtccagg aatctgtatt 42601 aaaggcttcc cagttgactc tggtggtcag agagatttgagactgattcc atcacattcc 42661 ccatctgtga acgggatcat acctagacag cccagccctagaactgcctg gtctgatcct 42721 aagtgttcca tggagatttt gtttttttaa tgtaatgaatgattaagtca gcaaatactt 42781 tacatctctt gtgtgctagg ccctgtgcca ggtggtgattatgggcccta ttctcaaagg 42841 actcctggtc tccgtctgtc acttacttta gaaacaagtcatctagccag agccagaagg 42901 gcactggaga tcactaatcM atcccatcat tacaYagctgaggaaactga ggcccagaga 42961 gtaactgcaa catgttaaag actacacagg gagcaaatttWttttctgct tYtaagccag 43021 gtggtttggc cacagatagg ccaagaagaa tacgaacagacaagccttgc tgggtttccc 43081 actcagttta ttactattag accattccct ttttccccactcacatttct gcgcatttcc 43141 atggtcttca tagaatctaa gcctaaaaat agagttttccttgagtcttt gggtcttcat 43201 ttYtgaagac tctcatgtca cagaaaactt taattaaataaatcgttatg cttttctctt 43261 gctaaccttt ttctacaggc atgtcagctg tgaYYcttatgatggggagg aaaggcaccc 43321 caccttctct gtccctacag tccYaatgcc ctacccagaatggggttttc aaaatcccag 43381 gacacccact ctttgtttct taagcactaa acctatatatagtaaatgtt tctggaagtt 43441 taacttcagc tcttgctgaa attttaaacc ctcaatttccagaggaccat cgccaggtgt 43501 ttttaaaaYc tcgataggaa taagaaatgc ttcccagaatggagacttSc atcagttctW 43561 gggagctatt agcccccWtg ccctggctgt aatggagaccgctgtgtctg cctcctttcg 43621 cactttggag ccaaaagagR aagggaccgc ctcccacgtccacagggacc tgacttccac 43681 ctctctgccc agatttgctt atgtcactgt cgccccgggacggggaggtg gggagctgag 43741 ggcaagtcgc gcccgcccct gaaatcccag ccgcctagcgattggctgca aSggtcYcgg 43801 cttggccgcg gattggtcac acccgagggc ttgaaaggtggctgggagcg ccggacacct 43861 cagacggacg gtggccaggg atcaggcagc ggctcaggcgaccctgagtg tgcccccacc 43921 ccgccatggc ccggctgctg caggcgtcct gcctgctttccctSctcctg gccggcttcg 43981 tctcgcagag ccggggacaa gagaagtcga aggtgagtgagcctccgggc cgggggccgg 44041 gagaaaaaac ctagcccctc ggtgtccagc gctcagtgcaatgcacccct tttcccaggc 44101 tccccgccag atgggcaatc cccaggtgcg agagacctcctgaacccctt ttgccgcccc 44161 ctccgccgcc gggaccccgc ccccgaccgt cgtcgtctcgtagttccatc tgttggagag 44221 ccgagacctg gtgcttcagg cgggcagaat gactaagggaggaaggtctc tctccccgag 44281 ctcgcacttt ctccccRctg ccacctcgag Rgtcgccttgctacatctat gtcacctccg 44341 tgcctgagct gctccccttd agtagggcct aagagggagggcgtcacaga aaaaaatgcc 44401 cagcgtctgg gtgggctgtg caagttccca gggagagagacaaggagaaa ggagagaggc 44461 agctgggtgg tcctggtcct gaagaactgc tgtggggggctcttctaccc caagaatgat 44521 acaggcaggt agaaatggcc acttggattc agggcattggtagaagaggc agagatctgt 44581 gctagaaRga gctctggagt gacagtgcag aacactcaggaccctgagtc cttacctgct 44641 cctggacccc cgtttctcca tctgcagggg agagtttgggggtgggagtg catggggcac 44701 aatgaagctg agggcctcag aggggatgct tggagaagctgaaaaatcca caagctgttc 44761 tctgaaattt cctcYcctgg ggcagcctca ctacccaggacccactgtcc tcattagcct 44821 gaggaaacct ggtagttgga gaaagctggg gactgctaattcagaagaag aggtagaatt 44881 ctctagagcc tcagtctatt tccagcaggt gtgtggcccaYacctggagt ggggatactg 44941 aggccaggag tgggatgacc cagcatgctt atctcctttgacagtatgta ctggggtggg 45001 ctctgYagac ctctcctcta cccagggagt gggagtgggtggggaaggag gcagaagcca 45061 ggtgtaggga tagactcaaa gcgagcgatt tctgtctYcaacttcccaac atgcataact 45121 cacatttttg caagctctga gcttttgttt ctaaaatcctctgggtcttc actttaatta 45181 agttSttatg agtctaggat tccgtttcca aaattctgtccacttctcag gaagcccatt 45241 ggaagcacag ggttccttcc atgttactca ccccttttcggtcctcagcc taaatccgat 45301 taatcagcct ctccttggag tcagtctaaa tggatgtgaagccacttcgt ccaagtcccc 45361 tcctttttat ccatccctgt tcataatccc caactcagaaggcattttcc aggtcaggaa 45421 tgggatcgat tttcaacatt gaggtttggg gtgggccatagacaggccag caccRtggag 45481 tcagtcccaa ccttcagttt tggaaRggtc aaagaagatacaattgggcc ctgcctcaag 45541 aaSgctagtg gggagaacgt ggactccatg aataactcatcatagctgtg tcttctctgc 45601 Mgcactctgg ggaaggataa atggactcag agagaataggatggggatgg tYtagaaaag 45661 ttttagggtg aggtgtgatc atatcacata caaccagtcaggacttgctg gctaatgaaa 45721 gcctgggcat gcccatctct gctcccaagt tcttaNagttcagatgtctt agctctgggt 45781 taggacccaa ccactcacct tacccccaac cccagggtaaagagaYaggg ttatggggca 45841 tgtggagaat ggaatggacg aaaggtgact cagccccagagaggtgtttt cctcctacca 45901 attgaaaggg agatgctagg gaagagacca ggtcctcctcttcttgctgc gtcacctccc 45961 cagtccaact tcaSgctgcc cacgctcctt gtctctacaaaaggactgca gaagccggaa 46021 agagtgacaa ctgctgacgt gcaYgggatc ctagtaactgctggtttcta ggtgactaaa 46081 cctggcagag agaatcatgg aaccacaggc tgacagagttaaagggccct taagacagcc 46141 ctgaacctga ctccatctta caatggagga gctggagccccagcaaaggg ggtcacatgS 46201 catcagagac acagcaaRgc Ycagcactca ggtctcctgactcccagacc acttctcaca 46261 gcatcatatt gataggagtR ataagacggg agaggagaaagacacaagac cagcagcccc 46321 agccagccag agtcctgNct gagtcccatc atccaactctcaagccattg gaaccctctt 46381 ctcttgcccg gctgtgggag gattcaggca ccctcatggcctctcccctt atctcctctg 46441 gccactgttt cagatgcggt gctctgggat tccacgtgcccagagaaggg aagggggctc 46501 atagaggact gagttgcagt gatcagtggt gagcacagccgtggctctcc acccatgggc 46561 caccttctga cccccactat ccYttgacat gagatttcgttccaagagac tccaggcctg 46621 ttttgctctg ccagcagatt cccacaaccR ttttgggcagcagcatgctt atctccctgg 46681 acatatgccc agacagtgta agtgacttgc tcagggccacRcagccagca aaaaatagaa 46741 cctaatgaaa tagggttctc cttgccacac ccccaagctgaaactgtgcc cagaagctgg 46801 tccttctStt tacaagttcc cattattagg acaacctgtaatcttttttt cctccatgtc 46861 tcagagttca tttttggatc atggcagagt taaaaaagtggagaggctat agacatgcct 46921 ggctttctgg ccactagatc tttgtggcgg tctagggtgtatttgttgtg ccccagtttg 46981 tctgcatttt aaggcggaac tggccctggc tgggaacgaggaggtccagg agcagtagga 47041 atgttgatct taggtcttgg gcctgcctcc agcacccaccaaaagtcacc ggatgtggaa 47101 tattagactc atgtaaagca ggaagtttgc accagagccaagaagagagc ttgcagctgc 47161 cagggaatgg cctggcggag gaagcgcagc ctcgcccagctcagcagacc tgcttcatga 47221 cagaggcagc tcttctgcac tMtactgggg ccacgccctgccagcatata tcactccctt 47281 cacctggtca ggcccaggta ccttgcctca acagcaagttctccagaatt gaggggaaga 47341 gagggagtga atttgcttct ctaactccag ccacctgtctctgccctctc ctggtctata 47401 atgctacttt gcattccatt tgtttattgt ctgcccacctctcaaaggat atcagggcca 47461 caaaggacct taaagaccac cttatacaat ggtttcaaggtttctccttt cYcatcacga 47521 ccagcccacc tgccaaattg tgtcaatgat gcaatgacatcctttcaaga atagtctcag 47581 aattttgttg tctgtgtcaY gggcagagta ggctcataggccacatccta ctagccagca 47641 gtgaccccaa tcccctctct cccactgatc atgatcctatcaagccatca tggttgacct 47701 ggttgRagag ttggttcagt tcaaggttct cattttataaatgaggaaac tcaggtccga 47761 agaaaagaaa gggccagtcc aagaccacac agggagttaatggcaRaggg acatggagcc 47821 caggtctccc agcccacagt ccactgcact ctgtccctccttgtcctgga ggctgactgt 47881 gagcagcagc tggggaggga ggaaggcata agggtcatcaccagcctgct cagacccgga 47941 acattaacac atgcaggtac tgttgctttt ccctaccKcaccacagccct ttcccccatg 48001 cctggctctc agtcctgctt YcctRtttgg taacaacagcttcatctttc ctgtcctgca 48061 gattgggagc ctctgaatca ccatgtccat acKgcctcttcctcctttct gttcagtttt 48121 tccttcacac tggcctagtt caggttctca tcctcaattgcctggattat tccaacagcc 48181 acccaaccag tctccatcag cccgtctagg aacccagtcatcagctttag ggcagtctcc 48241 ctgagagccc agtaaatgtt ctgaaattga catgtgatgtggaagtaatt agcaaaataa 48301 gtcattctcc agggttccat agcactcact tattaagtgcttaatattaa taagtgctta 48361 ttaggtgctt aatattaata agtgcttttt aagcacttaatattaagtac ccRaaaagca 48421 cttattaagt gctttcataa tcattatctt aaacaaggaattaggagacg tcagtgctag 48481 tccagttcca attctcggtg attactttga gaaagtcactctctctgttg ggatctttca 48541 ttcttttgaa aacggagatg tccaagttcc tttccagctctaactgctcc ttttatggcc 48601 tgtgttccag atggactgcc atggtggcat aagtggcaccatttacgagt acggagccct 48661 caccattgat ggggaggagt acatcccctt caagcagtatgctggcaaat acgtcctctt 48721 tgtcaacgtg gccagctact gaggcctgac gggccagtacattggtaaga gcccaccctt 48781 cctccctgct ttatttgggg ctgtatggca tatttcaatcacaggagctt ttctggtgca 48841 tgggggaaag ggtgatggca atcacgagag tccaagccccttttctcagc tccactgtgt 48901 tccgtggttt tgtgaagatg attatataag ccYgaggtctgattgccttt ggacatgttc 48961 taggagattc ctagttatcc ttcttcatct ctgggcacctcaacaaccct aaaggcagag 49021 ggatagagat taggtttgtg cttagagctc cctttggctggagcatgaga tggtaactga 49081 agctccatct tgctgaSaat atctYatttt ccctcagccccatctgcttt ggtgcttctc 49141 ttggcagctc tctggaaagc aggcaattgc ctcgaggccccagagtgtat gcgtggtgta 49201 gcgggatgaa ggagcaggct acacttctaa tgcccattctaaaataaact ttgaagatga 49261 tctagtttag ccttcccctc ttctcaatct acacatgggaaaccaagact cagagcgaag 49321 atacaactta aacagggtca caaagctatt agcggtggaatggggcattt ggcgcaggtt 49381 tcatgatctc ctgatatgct tactcctttc tccctagctggggtagaata cagaggctag 49441 gggaacaggc aaccaaaggc aagcgacagc acccaggggaaaacgctttg gggctgagca 49501 gtctactggc agggggtcag ggaggcaagg gcaatttgaccctccatgct ctgcctggca 49561 atcacaggcR aattcccgga agtgaaagga Yggcggtcacgtggcccagt ggctgtgagt 49621 gctttttctg ggatgcttct ctctagcctt ggaacagagcagagtgcgtt tgggcaagag 49681 atgggctggg tgctgctgca aaaSWgacca ggggacactgaggaaactgg ggagctgggc 49741 cagggcctca tctgtgttcc tgatttgcca aagattatgggagggttgta acctcacYcc 49801 aaagaagttc tctgccttgt atccagggat ggatagttcttgctccagaa aactcaatcc 49861 tgagggttct gaatgagtgc tctagctggc ccaggtggagggccttggca atgggcaggt 49921 gactgacagc tgacttgagg aagggtatta ttcttgtcctccaaacctcc aatccatcca 49981 gattagagtc aagaggatag gacctgagtc ccagcattgctgtgaactca ctggtgatcc 50041 tgcgcaagtc cctccctttc tcgggcctca gtagttccagcRgcacagcg ggtgagccgg 50101 gggagtggtg tggatgagac agggctcctc gccaggatactcccacRtct gctctttctc 50161 tttggcccag aactgaatgc actacaggaa gagcttgcaccattcggtct ggtcattctg 50221 ggctttccct gcaaccaatt tggaaaacag gaaccaggagagaactcaga gatccttcct 50281 accctcaagt gagtactcac tcagcatcct gagaaagctcctctcacatg gcccacatct 50341 tgttatcaac cccaaWtcat ggtggacatt tatcggccaccaagaactac tctcctcttc 50401 taggatcccc agtggaatga gggaagggaa gggacaagagagggagaagg acagggacaa 50461 ctggttgtga tgtgcatccg cagggagcac caaggttgagggacactgaa aagggaccag 50521 gctagaaagg aagaccgtgg actcacatta tgcctatgcccactgcacat tcactggctc 50581 ctgctgccca ctgcagaata aatccagact cccaacaccctctcccctgt tctgtccctt 50641 cctctcattt ctgagccctg tgcccacctc cttgggacccacctaagaac atttctcaac 50701 aggtatgtcc gaccaggtgg aggcYttgtc cctaatttccagctctttga gaaaggggat 50761 gtcaatggag agaaagagca gaaattctac actttcctaaaggtaagtga gctgccacct 50821 gtgctggctg gggctgcagc ccctcctggc tccagcccacagcgtcaggg cccatgccac 50881 ctcccctgct cctgggctct tggggaattt cttggcacctgactattgtt ccaactagag 50941 ggctctgcag accctgacta gggtctcMtt ggcccattttacagaaaggc ccagaaggac 51001 ccagagtgaa catactaagg gtctcacaMt cttctagagccacagctggc gctggcagtc 51061 ttctaactcc caaactgggg ctcttYtctc agggccaggctattccccag gaaggcctgg 51121 gaaggaagag ggtcaggggg cctcaagcaa ggttgacactcctctYatcc ctgctctaga 51181 actcctgtcc tcccacctcg gagctcctgg gtacatctgaccgcctcttc tgggaaccca 51241 tgaaggttca cgacatccgc tggaactttg agaagttcctggtggggcca gatggtatac 51301 ccatcatgcg ctggcaccac cggaccacgg tcagcaacgtcaagatggac atcctgtcct 51361 acatgaggcg gcaggcagcc ctgggggtca agaggaagtaactgaaggcc gtctcatccc 51421 atgtccacca tgtaggggag ggactttgtt caggaagaaatccgtgtctc caaccacact 51481 atctacccat caYagacccc tttcctatca ctcaaggccccagcctggca caaatggaKg 51541 catacagttc tgtgtactgc cRggcatgtg ggtgtgggtgcatgtgggtg tttacacaca 51601 tgcctacagg tatgcgtgat tgtgtgtgtg tgcatgggtgtacaRccaYR tgtctRYcta 51661 tgtgtctttc tgggaatgtg taccatctgt gtgcctgcagctgtgtagtg ctggacagtg 51721 acaacccttt ctctccagtt ctccactcca atgataatagttcacttaca cctaaaccca 51781 aaggaaaaac cagctctagg tccaattgtt ctgctctaactgatacctca accttggggc 51841 cagcatctcc cactgcctcc aaatattagt aactatgactgacgtcccca gaagtttctg 51901 ggtctaccac actccccaac cccccactcc tacttcctgaagggccctcc caaggctaca 51961 tccccacccc acagttctcc ctgagagaga tcaacctccctgagatcaRc caaggcagat 52021 gtgacagcaa gggccacgga ccccatggca ggggtggcgtcttcatgagg gaggggccca 52081 aagcccttgt gggcggacct cccctgagcc tgtctgaggggccagccctt agtgcattca 52141 ggMtaaggcc cctgggcagg gatgccaccc ctgctccttcggaggacgtg ccctcacccc 52201 tcactggtcc actggcttga gactcacccc gtctgcccagtaaaagcctt tctgcagcag 52261 ctgagcctac tgtgtgtggt gcttcttcaa tggtggccgcccccgcctgg gtgggaagtg 52321 aggaggagaa ggtggggcag ggtaaaggSg ggaggatgagaaaagaacaa atatgacaac 52381 aaaaactttt tacagtgggt gcaaaggtaa ttgcggttttYgccatatgg caaaatccaa 52441 ggctacctcc ccaccccaca attctccctg agatcagccaaggcagactg caagggccat 52501 gtagccctat taggggtggc atcttcatga gRgaggcccccctgccacaa aaaaggggca 52561 aaaattgcaa ttactttttc aacaacctga tagttagctgcagtggcgca ctccttcagc 52621 tacttgggag actgatgtgg gaggctccct tgagcccgggagtttgaggc tatagtgtgc 52681 cRtgactgag tctgtgaaga gccactgcac tccagcctgggcaacacagt cagactgtct 52741 cttaaaaaaa aaaaaaaaaa aagagagaaa ttagacaatgctttgagtcc ctgctttttg 52801 gctcctacct tggcccactc tgcttctctc actcagcatttggagtttat aaaaccagtt 52861 ctgatctact ctcattccct cccacagttc tccaagtcagctgaggttaa gatgaggtgt 52921 tctgtttgac ggatgaggaa actgaggctc acagaagggccacgccttgc cccaggtctc 52981 ctgatgagtg aggggcagga ctggaattta aacccaagtcccctgagtcc tggcctaggc 53041 tccttccact gccccggYtg ccctaccctc agggaggccctgatacgtca ccattactca 53101 agagtaatgg tgaccatctc agggaaagga agaaaaggggaacagaagga gggacccagg 53161 aaagcagggg gttgacaggg gaacatYagc gcataaatgaacatgagtca gaatgttgat 53221 cttcttcaac ttggatttat gtccatgatt cgtgcaaatagctatccagg gatggacagc 53281 caccctaatc tgggcttctg gcaccacagg cctccagctatggggtccag ggtctgaacc 53341 tcagggcctg gcagcttcag gctggcgccc ctcggcRgacgtggctggca tggccttctc 53401 ccatctgtga tggcttcagc aaggctactg tgagtggtggtggagagggc cccagagcca 53461 gaatatccat cattctcttc tgttctggag acaaacccacacttcccaca gctcctcaca 53521 gaggggtagg ggtgtgcgtg gggcaggtcc tgctacagagcttgaagcaa aaattaaaca 53581 cacacacaaa ttggtcatgg caactagagg gcctgaaaccacttccggga ggttttgaag 53641 gaagggggtc ttggctgcct cccactctta ggattgctgctcctggaggc ttctgcaaYg 53701 gatggtgggt caaagccgga tgaggcctct ctccactcagcagcagggaa ggagtttttc 53761 ccagtcactc ccagcagagt acaaatgaaa gccttctgggtggagcctcc ccagtcctgt 53821 aaacagctca gttcagggac tggtRtacaa gctggccacccatctcagcc tctcatccag 53881 ctgaggctct ggccacaccg tgcaagtggc ttctagtttYttggcaatMt gagatcagct 53941 ggctctgcaa gatgaaggtg gagccaaatg acacaatctggtctcactga ggcccctcac 54001 ggtcattttt tggagactct aaataaacaa aaattttgaggacttcatga ttatgtggta 54061 gagcgagttt caaagtctct tatggaatgc agtgcaggaacagtgaaaat aacagctagg 54121 gttactgggt gctaccaagt gccagggcta agtaccttaagtacgctggt catgtaatct 54181 tcacccagcc ctatgaagaa ggcaggttat tatcccattgtaaaagaggg aactgggaca 54241 ctaagaggtt ttaaatgact tgctcatagt tccaggtccaggaaatagtg gggctaggat 54301 ttgaacccaa tctcaaatcc tggagcagtt ctggggagggagcccctccc attataccaa 54361 tctctaccac tcatcaaagc agcctccaag gggctccactgaacttgtag ggttcttcag 54421 ggaaaaacag aaaatgactg agcagcccaa tcccttcttccaatcaccaa atcctccaaa 54481 ctgcaacccc tccaaggtgt ccgtcccatg gcctctactcacacacctgc aaagggacac 54541 tcgccacctc ccagagctgg ctccatttct gcacaactgtgtcccttaga aagaaagttc 54601 ctcttcatgt tgcgatggac ttggcctccc tgtcattcccacccactagt tataattctg 54661 ttcccagaag tcccacaggc gaggtggtgc cttgtgccccatgagagctc tacagggagc 54721 aatggcagtc gctaaggtcc caatcttttc acgagtggacacagctccag gctcttcagc 54781 ctttcatggt ggacaccatg gtcaggggag cttttcaaacagaaatatgg tcatgccact 54841 ctgctttaaa aatgtccagt gactacacat tatgctgaggataagaccca ggcctcccac 54901 catgctccgc aggcctgcac ggtcaggacc cggctacgtcctgaaccctg cgttgcccct 54961 ctctttccct accttgttct tggctccagc cacgctggtcttccctccga tcctcaaatc 55021 agctccccta cttcccctcc tcccaccacg gcctctgcatttgtcatgtt ctctgcaaaa 55081 gcatcctcca cccctacctc tccacctagc tagtgtcactcattctgaga gtctcaactc 55141 aaatgctgcc cccccggtct aggtcggctt cctctgctatctgctcccac agaaaccggc 55201 tcttcttcct ttagggcact tgagtgcagc ctgtttgtgtggtgtttgtt tattcatttc 55261 tgcctccctc attagacatt acgctgtata agggcaaggactgtatctgc ttctgttctc 55321 taggcatcca tagcacctag cacaatgcct ggcattagataatgcactag aatgaatggt 55381 ttcagccccc ctcactctgt ggttgccatt actctccattcaacactgtt ttggaaagct 55441 gggcctctcc tccaccaaga gcagaaacta acRacatcacccccaaactc aggcaatgct 55501 ggcagataag atattatctc ccccagggag gaccaagactcagtattagg ggctcacctg 55561 gttctgtagg cctggctgtg ggagggtcca tcacttgggagctctgattt ggatttcgaa 55621 cccctccaca gggtagacgc caggtgtatt ccggctgcgacaaaactggt gcttaataat 55681 caataatcaY acccaaaaat tacaatagca cctactgtgccaggacaata ctaaatctgt 55741 attagtcaag tataattgaa tctttataac aacccaggctggcactccca tttcacaggt 55801 aaggaatctg aggctcagag gggataactt gctgaaggtcatctagcaaR gagaatggct 55861 aagcttggat ttgaatccag gtctttgtaa atccagagccaatctcttaa gcgccatact 55921 atatactctg gtgtttaaag atgtgtacca agaagggtcatatgggagaa gctgaaaaag 55981 aggccagcca ggaggtttct gtaatggtcc aggggagagctgggatgcct aaggtggggc 56041 tgtggcagag gactggggaa aatgggacaa gtgacagcagatctttggaa gaagaatgat 56101 aagaagggaa aagaggccag gcgcagtggc tcacgcctgtaatcctagca ctttgggagg 56161 ccaaggcggg tggatcacct gaggtcagga gttcaagaccagcctggcca atatggagaa 56221 accccgtctc taccaaaaat acaaaaaatt accgggcgtggtggtgcatg cctgtaatcc 56281 cagctacttg ggaggctaag gcaggagaat agcttgaacctgggaggcag aggttgcaat 56341 gagccgagat cgtatcactg cactccagca tgggcaaaaaagagtgaaat tccatctcaa 56401 aaaaaaaaaa aaaaaaggaa aagaaaataa aaaaagagaggagagaagaa gaggagggga 56461 ggggagagga gcggagggga ggggagggga gaggaaaggaaaggaaagag agagaaacat 56521 ggtgaggctc tggaacctag ttacacgtgg aagttataagaaaaagcaaa cccaaactct 56581 gatacccatg gcctggggcc tggagggagt cctccttcccaccctgcact cttactcagc 56641 cttctccgtc ctagaccagt gaccctgaac agctgtggggcagccctgcg ggactgtggc 56701 cagatccctg cccctgggta gcagcccaga gccaggccctggacacccac ctgccaccac 56761 Rgtgctgttt agttcagaag gatgcagttt ctgaacccctcacttaacct tcctctctgg 56821 aaggtgtctg gggcagcctS tgtgtgtgcc ctggagcaagggagggacac agactcacca 56881 gatggaagag gcgcgagttg gggagtgggg gcgggtgctccatggccatg gggggagggg 56941 ggtagcggat ctgggaccag tcctcgaagc catggtgtggcaccatggct ggcatgggcR 57001 ggtaggcgta ggggtaggcc ccgcagagat gttctgggtggggctccaca tggtaacgct 57061 ctcctgaggc ctgcaagaaa ggctgacgtc tggctttggcctgcaacagg ctgcaMagga 57121 cacccatctc atcaccctct aaatagaatg ttagctctctgaaggctagc aggctgggta 57181 ccaaaggctg gtatgtggtc atgaaaggct gctacatgggtacagaaatc tggatacatg 57241 ggcatagaag gctggatata tgagcatgga aggctgcatgggcatggaag gctgggtaca 57301 catgcatgga aggttgtgac atgggcacag aaggctggtacatgggcacg gaaggctggt 57361 acatgggcat ggaaggctag tacatgggca cggaaggctggtacatgggc acggaaggct 57421 ggtacatggg cacagaaggc tagtacatgg gcatggaaagctggtacatg ggcatggaag 57481 gctggtacat ggacacggaa ggctggtaca tgggcacggaaagctagtac atgggcacag 57541 aaggctgcta catgggcagt gaaggctggt acatgggcacagaaggctag gtacatgggc 57601 agtgaaggct ggtacataga cacggaaggc tggtacatgggcacggaagg ctagaacatg 57661 ggcacagaag gctggtacat gggcacggaa ggctgggtacatgggcacgg aaggctgggc 57721 acatgggcat ggaaggctgg gcacatgggc acagaaggctcatacatggg cacggaaggc 57781 tgggtaaatg gacacagaag gctgggcaca tgggcacggaaggctgatac atggaaacgg 57841 aaggctgagc acatgggcat ggaaggctgg tacatggacacggaaggctg gtacatgggc 57901 atggaaggct gggcatgggc acaaaaggct gggcatgggcacaaaaggct ggtacatggg 57961 cacggagggc tggaacatgg gcatagaaag ttggatacatgggcacggaa ggctcataca 58021 cggccaaaga aggctagtca catgggcacg gaaggttgctacctggacac agaatgtcaa 58081 cagtgatgac agctaataca ttgtgggcac tgcctctgtaccaatactgc tctaaggctt 58141 ttcctgtatt actcatcagt tagttagcag aggatgtccccacgcccgag cacacactgt 58201 gcatccatca ggctagctcc atgaggaagg taagaggatgtcagtgctgc tacctccctc 58261 aagcctcctc acctttgctt tcctcttctg ctgtctgagggcttctcttg ccttctcctc 58321 atctttgaat gcttttagct gagagaagaa gggagggagaaaagactgtc ggcacaggtg 58381 gtttctggcc aggcctggat ccagggccta accagccaMRaggactgacc ggctgatgct 58441 tccctctggg ccaccatgcg tgggccagcc ccgggaaagggccctgaggg cggagcacag 58501 cccaggctgt tgctgcaggc cacctgcttg tcacagtggtctgtgcacag gtcaagaggg 58561 ttcacgtgaa cctcgctgct tctctgctaa catcttttagttccactctg ggagagtaaa 58621 gcccagaact gactgtggaa agtcagaggt agagatatcttagagtaaaa ctcgtaattg 58681 tacaaagaaa gagtcagaga ggggaagggc cttgccgagctcaaatatga ttgcagagct 58741 ggagagaagc agtgagggtc ctgacatcca gcctcaccagggccctccaa tccatgcccc 58801 ctctcccSac gaggacaggc cagttgccct tcctgggtccagtcactctt acctgggcat 58861 ttgacagggt gacctgggcc tgcagcttct ccacttgcttcttcaRctct tccttctcct 58921 cattcatgcg ctcacgatca ctgcgctccc tctggaagtcctcctcgaag atcttcacct 58981 ggtgtggagg gagggtgaag agagggaggg ggatggtcctgagtggggat ttctcctcag 59041 gcccagaYtc ccatgcctcc tgctggggtc acagatggcagagctggggg tcgccatggg 59101 gagtgggaca gctacacctc cagcctccac cttaacttcaaacagagtag ttctgtgtcc 59161 ctctatctta cattctgggc ttcaagStag gactttatttgaacaaagga tatatggttt 59221 taaaaaaacg ttgaaagcca tcaaccagtg caatcctttcactccgctga gacccacagg 59281 ggcagtgatc acagaaccag agtgtggtgt agaccagagtttggatctgc tggttctgag 59341 ggcagagacc ctacaaactc Ygctagggct ccagggtgccctggcagagg tgggttgggt 59401 ggggcctgcc tctgtctctt agcctgtagt ttcacatccttagtgcagat cagctgagac 59461 actggctagg ttcaaggcag tttaggagga ttaaatagcacagagacaat gggagagcca 59521 caaccttttc gctttctttc accccaatcc ttctgtgtccctcagggcta gtaggtcttt 59581 aataccattt tagtacctgc caatctcctt taaataaaggagaaaagaac tccagttcag 59641 agtcttcatt tggcaagagt atccggctag agtttaataacattgttctg tttttattgt 59701 ttattgcgta cagttacttt ctacttgtga caaataataaatggtttttt ccaattacag 59761 cagtgatata aagctccctt ttaaattcat ttatttagaatttatttttt aaatactagg 59821 taaataatag cacgtgtggg gcatagagat ggctaaatcataaaggtggt agaggaacga 59881 cttaagctag ggttatggtg ctggaaagga taaacctagagccagtgggg ggccctggcc 59941 aatggccaga aagtggatta ctaattacag gttccatgtgattccaagcc gtctgggccc 60001 tcaggaaagc tccagctccc acagagcacc tcccacctgatttcctcccg gaagcctcac 60061 ctgctgtttc agcaactcat tctgcgtgac cagctcctgtttccttagga gggcccctgc 60121 tccttctggg ctcccaaatg ctgttggtgg agatgatggcggggtttgga tgctcagtgc 60181 ttcctccagg gcctggaatc aggaaaagat gggaccatcagcaggaaccc ctggaaatca 60241 gggccctttg gaggggtcat cctcagggaa ctccttcctaataataatca tactaataat 60301 agccaccact accagtccta tttaataaat gctgccctatttatttgcat tctacacata 60361 tcatttcatt ggatcttctg cacagtgcca tgaagaaaagactatgatca tcatcctcat 60421 tttacagatg tgaaaactga ggctcagaga cctgaaggggcttgtccaag gtcaaggtca 60481 cacagctagt aagtggcaga gcacagattc aagcccagcacatgctccta cccagcaggc 60541 taaactgggg ttgtcatgca gaaggggaaa cctccccttccctgaacatg tgtggctcaa 60601 ttcttagcac ccagacctag aaggaagatt aagtcctccgggggaagctt cagtctcccc 60661 aacaactgcc ctgccctgag attcttcact ttcagcaacccattcccatc caaaccaccc 60721 cttccatcct caacacttat gtacatagag aagactggatttaatttctg cttagttaat 60781 attttccctt tctaacactg aaaatgggct gaaatatcatcaaataacat tttgaaaata 60841 aaaaaatact cttaatctat catgttcaca atagattatataaacagtgt gcccacatct 60901 tcttaaaggt ttcagttaac cgtgtttccc ctttaactctgttaacatgt tactcagaac 60961 tctggaacat gtctcaagac tgggcaccct ggactttggccccaggacag catcgcccat 61021 gggcctccct ggaccgaggg gagatagaga cctggcccaacttggactcg ggcttccacc 61081 tggtctcctc agctccccta gacacgcaaa gaaactgcaggaccgagagt aaattcagga 61141 tgctccgagg ggaagttatg gctccacttt atcttcctactccaccccac ttttaaatga 61201 aaagactgag gtccagcRac agggagatgc ctaggtcatgagcaaagaag gccacatgcc 61261 ctgcattact catccacatg gtgtctgtcc ctggctctgccaagaaaacc tccaaggtca 61321 tctttatgat cagcaacatg gacaattctc tgcctggatttagagcccca agtcccccag 61381 ctcaggacag atcgcgtttc tttctctgga aacaggtcaagggccccaga cttggcataa 61441 tcagtgttta caaaacacca aaaccaaaaa gaaagggtttccccagcagg cctaggaatg 61501 ctcaactcat gccagagtcc actgtcacca gggtcaggaggcagagggaa aggaaaaaca 61561 attagcacga aacgaaaagc tgagccatga acttaactcctccaggccct cagagaatgt 61621 ttcctttcaa agctccctcc tgtcagcttg catctttgaaatgtgctgga aagcaggcag 61681 aactagagcc gttcccaccN gggctcggat ggcaaaactgaactggagag gctggagagg 61741 ctaagctggg actagaccac tccgcttggc teagaccagagggtttctgg tttcctcagc 61801 ctctaccctg ggggccccat ctccctggat cccactgcacaagggcgggc ccctcagccc 61861 gtgctcctcc actcccatca gtgtttgctg aacatgtgcttgatgccagg cgctgtacaa 61921 ggcgcccagg gtatacacct tggtgaaagt atgcacctcccaccaccaga tgggaagaca 61981 gaattaaata agtgatgacg agccatgaga aggagtgtcagagaagttgc tggtacctca 62041 ggagcataga ccagggcaac ctgaatgaga ttctgggaaggctttgctga gcaagtgagg 62101 gtaaaacaaa ccctgacaag cagggtgggc aggtgaggcacaaggggaag agtgcacctc 62161 cagagggaag ggtgacacag gctaattcaa gggctgagtcactcccagtg tgggaggcaa 62221 tggcagagca ggcgggaaac aggatgccag ctcactgactgggggttacc cttcctaagc 62281 tgaatggttR ggggtgccag tgatggggtg ggcattgcagctggactcaa gggagcccag 62341 ccaagggacc cgggccaagg cacccacctt gttgagccgctggatctcct tttcctggta 62401 ctcacgctgt cgggtgagtg ggctcagctg atcctgcaggtacttgacct tttggcgcag 62461 ctccttggcc tctgctgtca gctgctcctt gtcggtctgcagggaataca aggttggtaa 62521 gctggctagg atggcctctc caggaggcct cggattctctgtcctgcctg gcccagccct 62581 tacgttctcc cctggYcatc acttcacaat gctcacatgcaaagcacctg gggtcggtac 62641 agtaatgtcc atccggtcct gccctcaatt attatgtgtcaatttaaaat aataattaaa 62701 aaaagaaaaa gcagtagctc agttgagtaa aagaccctggattccgacac cagttcatcc 62761 actaagcagc tgagacccct ggcactgcca cagccctctcttgtctaagc ttacctacct 62821 gtcacaggta cggggttcct aacgtgagtc acacagagacctcaggaggc ctgtgagccc 62881 caaaactgaa tgcgaaattt tgttgtgtgt aatctgctagaaagaaaagc catagttctg 62941 accagattca catgggggtc ttccgtgatc cacagaaagcgataaactac ggataatccc 63001 taacagacac tgcagctcat acagccttgg agcctagaggagagatgaca cgcccagaga 63061 gaactgtgac ataaggcaga gagtgctcct ccagaaagaaacaaaatact gtggggctaa 63121 aagaaagatc accacagctg agggaaggct tccaggaggcggttccatac cctctacgga 63181 accagcaggg cttcgccaca gccagccaag actgggatggcctttagaca gcatcaagcc 63241 ccagccctgt aatacgatgg gaaacaaatg tccagagaggggaagtgacg tacccaaggc 63301 cacacagtga gctggggcta gaacccaggt ttcctattcccttgctctct ctccttccat 63361 gtcaaaggag gtgttgaaac taggggatgg cagcacagtgcagtggaaag accaggcaca 63421 tccccaaata aactatatat tcctatgtgt acatatatgcgagaaaggtc tggaaacaga 63481 tgcaccaaaa tggtaacagt ggttccctct agggagggcctgggatcatg gcagtgggag 63541 gtagtccagg gaaactttca ctttgtagca tgtggacatttcataactgt gtattcacag 63601 catgtgatta agagaccagt cgcaggggtt agttttcacccagccctgtg tgacctttag 63661 tcagtacgga ctgcagaggg caggcttggt cagacctccctgggaattgt tctggactcc 63721 ccatcaagag gctcccggat gggcatgggc tttaccgaaattcatcctta tctacagggc 63781 cacgccttgt catgaaacgt cctaccttcc ctgtagctgacagggaagag ccctcacatt 63841 ccagggattt gtctcaccta ccactgtgag caagggagaggggccaaaga gccaggatgt 63901 ctggctgcga gtcaccaggt atgactgtgc actcataaggaaagacagaa gtgttctccc 63961 taccacacct ccaaggtaag ggggtgtcac agacgtaggctgactgctca aataaggccc 64021 ctggtgtccc atgctgacgg gaggaactSt gaaaggcaatccaggttgaa aatgtccatt 64081 tttaagctag gcaaaagacc atgcccagtt cccatgaaggggaaaacatg gtgacataaa 64141 taaaatctcc caagagtctg gcagtcaagt ggcctttcctctttaagctg ctccaaggtc 64201 ctgatgggcc ttggctggtc tcattcatac atatgactgggcagatacta ctacttcagg 64261 ggacctgttg tttcccccca agaaagaatc tggtctcacttatgcccact ctctgatctc 64321 tgtaacttct tcttcgtagt actttttttt tttttttttgagacagggtc tcattctgtt 64381 gcccaagctg gagtgtagtg acacaatctc agctcactgcaacctccgcc tcccgggttc 64441 aagcgattct cgtgcctcag tctaccgagt agctgggactacagacatgc gccaccactc 64501 ccagttaata tttgtatttt tattagagac ggggtttcaccatgttggcc aggctggtct 64561 caagctcctg atctcaagtg atccgcccac cttggcctcccaaaatgctg tgtttacagg 64621 catgagccac catgcctggc catacatttt tcttttagagaaacagggtt tcactctgtt 64681 gtccagtctg gagtgcagtg gcacaatcac agctcactgaagcttccaac tcctgggctc 64741 aagcaatcct cccacattag cctgagtagc taggtctacaggtgcacact gccacaccca 64801 gtgaattttt taatttttgt agagacaagg tgtggctatgtcactgaggt tggtctcaaa 64861 ctcctggcct caagcaatcc tcccacctcg gcatcccaaagtgccaggat cataggcaca 64921 agccacccaa cccggccttc tctgcaacac ttaacacacttttactttat ttgcttctgt 64981 aattagttgt ctggcacctc tttcccagcg agggcagggttgagctgttc ctgtggactc 65041 tatgccaaag cacaatgcct gagacaggct ctgaagacaaatgccgggat gaatggataa 65101 atgaagctct gtctcatgtt gtagcccctc cccaacaaatcaccatcact atgctgaaca 65161 cttggcagca tcccacccca ccaagtgcca tacagcaggcctgcttggat cttttttttt 65221 ttttttttta gcaagagttt cactcttgtt ccccaggctggagtgcgatg gtgcaatctt 65281 gactcactgc aacctccgcc tcccaggttc aagcaattctcctgcctcag ctacctgagt 65341 agctggaatt acaggtgcct gctgccacac ctggctaagttttgtatttt tagtagagat 65401 gggatttcat catgttggcc acgctgatct tgaactcctgacctcaggta atccacccgt 65461 ctcggcctcc ccagttgcta ggattgtgag ccatcgtgcccggccacctg cttagatctt 65521 ttatatggtg tgcttccctg tacagttccc cattccctacaattttaatg gaacaaaaag 65581 ttctgaataa aaacaagaaa atgtgaaaag ctgtagttaatgtaactttc tcaattttca 65641 gatgggaaac tgcagtgcag ggaaggaacg tgcctgtgtttttggtcacc tagctctttc 65701 gcactagacc cccgggtctc ctgactcctg cccctggcttgtttgctcca cagaactctc 65761 cgctaatgtg gaggggaact gactctgcag ggaccagcagtcacacttgc ctcctccatt 65821 tcaatcttgg acttggccag gaggagcttg cggtcaaagtcacgctgctt ctgctcccgc 65881 tcggcctcca ggtcagtcac ctgcttctgc aaatcagccagcttctgacg cagctcagtg 65941 atctgggttc agaggcagag aggagtgtgt gaggcaaggatgtagagcac ttgcaggatg 66001 tgggcagggc ctggctgccc ctgggccctc caacactgcccccaacttct cctctgggcc 66061 atactggggc tcagcgKgKc cactcYccaa ggttcaaagctgctaaagag gcagMgagat 66121 gaagaccagg catgccctgt gggcgtggcS aggaaccccacattaccttc tgctcatact 66181 gctgcttcat ggaccggaaa tgctggtccc actgcttgttcacttccagc agctgtgggg 66241 agagactgga gttagcagga gatgagcaga gaaggaacaaggagagccat ctcctgcccc 66301 atgtacacca gcgtcccaag gtgtgctcgg tgtgtgtgaatagtggctac agacactgtc 66361 tccatccctc agctctcgaa aactcaatgt tgctcaaagccatccacctt catccactgc 66421 tgcattttac cttgccagga aagcaagatt aacactaagacatggaaact acatMgtttt 66481 ggtgaataat tatggaacac acacacctct ttggagggcagtctgggatt atgtattgaa 66541 atttcacatt ttcatcaact ttaaaccaat aattccactctgaagaattt aaccaataga 66601 aacacttaca caagtgtgca aatatatata cacaaggctaatcatctcag caatacctgt 66661 attacaaaaa aatccagagg ccaatctaag cacagaagataggttaaaga aacgatagta 66721 taaacagaca atgtgcagct gttaagaaaa taagaaaataggccgccagg cacagtggct 66781 catgcctata attccagcac tctgggaggc caaggtgggccaattgcttg agttcaggag 66841 tttgagacca gcctggagaa tatggcaaaa ccccatctctacaaaaaata caaaatttag 66901 ccgggcatgg tggcacgcat ctatagtctc aactactcaggaggctgaga tgggaggatc 66961 acctgaactt aggaggtgga ggctgcagta agccgagatcatggcactgc actctagcct 67021 gggcaaccaa agtgagaccc tatcttaaaa aaaaaaaagaaaagaaaaaa agaaaacaga 67081 aaataaagca cctaacacac ttcctcagtt tgaagatgacactatccacc atactcttga 67141 tataacaatt cctcagggaa gaaaaacaac cctacatcatcaactaaata aaacaaaagg 67201 agtattggcc ctgcccctta agatatgtca taaggatccacaccaatttc aaaaacattg 67261 catatgataa aaaaaattac aaagaattaa aggaatatagcatatgttat agatatgaat 67321 agatactctc aaaggcagtt aatttttttt ttggaggcaggcctaggctg gagtgcagtg 67381 gtatgatcat ggctcactgc agcctcctga gctcaagtgatcctcccacc tcagctcccc 67441 caagtagccg agactacagg tgtgtactac cacacccagctaatttattt tttgtagaga 67501 cagggtcttg ctatgttgcc caggctggtc tcaaactcctggttgcaagt aatcttcctg 67561 catcagcctc ccaaagtgct gggattacag gtgtgagccaccacgcctga cccagttaat 67621 tttttaaaaa agaaaagaaa aaataatctg taaagcagtgtgtgtatata catatatata 67681 cacacacgta tatgtataat tttattctac tttgaaaaaaactgagggat attcaacaaa 67741 ctgtaatcag ttgtggtttt ctctaggagg tggtattcttgatctttttg taatgagcat 67801 tatatcactt ttgaaatcag aaaaaaagac gcttataacaatacaaaaag aaatctaaaa 67861 ctaatatgca catcaagaag ctttcaaatg tgcgggaggacaaggggagg caggctcagg 67921 gttctagaga agcacctggg ctgtgtggag aggggctagacggaacagac agaacaagtg 67981 tctaccaaaa aggagctgca cttcgacctc gggtctggaaagactggact agactggggg 68041 tgtgaggagg gggaggaaca catgtgagat ccatgggaagaggcggaatg gactgcagca 68101 cagggactct gctgcgtggc ttctctcttg gcagcagagggaaactaaga gcctcttacg 68161 aactggctcc tgcaccttag tgggactggg ggacatttaaactacctaaa gacctggcca 68221 tcgccttaat ggagtttagg aatccactca gtattctgtggcaggcttcg gctgctggct 68281 ggtccttttc attcttgcct aagaaaattc ttgaaatgggagtctcaaaa tatctgcaaa 68341 gtagctacac caatagaggt aggtagctat ttttttttttgagatggggt cttgctctgt 68401 tgcccaggct ggcacgcggt ggcacggtct cggatcactgcaacctcagc ctcccgggtt 68461 caagtaattc tccctgcctc agtctgagtg ggtgggattacaggcatgtg ccaccatgcc 68521 cgctaatttt ttgtattttt agtaggcaca gggtttcgccatgttggcca ggttggtccc 68581 gaacctctga cctcaggtga tccacccgcc tcagcctcccaaagtgctgg gattacaggc 68641 atgagccacc gcacccagcc aaggtagctt ttaacgttaaaaattttgac agtaaattta 68701 tggagcctcc aggctagtgg gaagcggcct taatcccttccttccatgat gggtcctccc 68761 taaagcatct ctcaagggtc cctccactcc tcatcggtgatgctaaaact tgcctaactt 68821 atcacaacct ctttccacca tccaaggtct accataggagcctgcctaaa ctgtagcccc 68881 aacaatttta ggccatgccc cacatgccac ctcctcagagtgaccactgt aggcaaaggg 68941 actgatcaag agaggacagt ccagggcaag gagtcagacctcatccatgg gcaggcaggt 69001 cagtggtcac catgccaact cctcatggtg acctgggacctgccagagcc aggaggocag 69061 ggcaagcctt tgtgctgctg gtcccgggag gtaagagggatcctcccact actgccacct 69121 cggccaccta cctcactgcg ctgctgctcc agcatcttcaccttcttctc ggctgcRccc 69181 aaggccacca cctctgggac cttacctgcY gtcacactagcctggggaga agcacagagg 69241 agccttcacc aaaacctcaa tacaaattat ctcacccagtctcggtgctc ctctaagacc 69301 cctcacccaa gKggccccag ccctctgtct ccctattcctcccacccagc aggacaccac 69361 taaccatccc agtcatgact acgtcccctt ctagaagtctacaagatgat gattccccct 69421 ccctgactca gggtggctgc caggccctct ccccttcccttctcccctcc ctacggtcat 69481 tctccctgtt taggtacaac ctctaaggta ctaccagccactctcctcac tccctacaca 69541 catcagccaa aaaactctct ggcctggaag gtaagacatttgagatgtag atacagacag 69601 atatagatat agatatactc attctataaa acatatatgttttataaaac ttttacaaaa 69661 gttgaagtta tataaaatgt ttatttaaaa acatttttagaaagatgcag aaataaagat 69721 ctgtgtaata cacatagatg tttcccagct ctgtcctctgtaaggggcta aagcagagac 69781 atccagcagc actgagcaaa cctagtgctc agttggtttctaaataccat tttcttttta 69841 ttaaaaagaa acagggctgc tttgaggggc tggagcagataaaatataag gtgagtttga 69901 tgcatcttat agtgatggaa agtaaggaag tgctcaaatgaataatacta ataacaataa 69961 taataaaaga gcatgtcaaa agggcacaag agacaacctgaaaaaaacat ctatggccaa 70021 aacgggaaca ctttgaacag caaaatacat cacaatagtattggattata agtcacagta 70081 tgaaataaat actcttgagt tcatactgat agaacaaaatagctgaatca ataaataaat 70141 gggggagaag ggacagctct tccttacaaa agaattctgattaataagtg taggaagaat 70201 gaggaaaata tgaaatcacc attccattac tacagtaataactRctgcag gtggagatcc 70261 accaatggtc actaaaatca gtgagtgaaa gtttaagaaaaaatagagta tttgcatagt 70321 ctctaagttt ctcctcaaaa ataaacactt attaattacaaagggaaaat tggtcacttg 70381 attgcagaaa cttgaaagac agcacttggc caagtgatgaaggttaacag tagtaaaaca 70441 ctgacatcat gtacccctgg tctgctggac tgagaaatattctgcacact ctgatgtaca 70501 cagaacgtca cctccgtgag attcttgcca aaacacgtaacctcaatctc ctcatgagat 70561 ccaatcactt gtgaggcctc aatcgtgaca gcaaataagtctatactgaa aaacattcta 70621 caaataactg gccagtacaa ctcaaaagtt tcaaggtcatgaaaagcaag gaacagctag 70681 gaactgtcac agactagagg aaaatgaggg agaagagacaactgaatgca atgtgggatc 70741 ccagactcca tgctggcaca cgacaggaca tgagtgcaaatctggtgaaa tctgaataaa 70801 gtctgtagtt tactaagtag tacatgccat taattcctagttttgatcat ttaacctggg 70861 ttctataaga tgttgccgtt aSgggaagta aaggaaggggataggggaac tctctagact 70921 atttttgcaa ctcttctgta agtctagtta taccaaaataaaaaggttta aaggttaaaa 70981 aaaaaaatag caagtgctgg ggtgaaacag cagacaaggccagtagtccg gagggtgtgt 71041 gtcgactcta tctccaagcc cctctgtgat ggacaccaagaccctcgtct tctctgggct 71101 tcaatgcgat gagaagctca aacagaagaa ggtctgcaggccccatccgg acttctgaca 71161 ttgtctgcca tcatcctgga gtcagattct ctctattctcagcgttctct ttccagcgtt 71221 ctctttccct ccacactgca cccccaagtg gcttcaggtagggctgggtg cagagcttgc 71281 cccctacagg ctcaggcctg taagcacacc atagcaccaaggagcaaggt gagggggagc 71341 cctccaagga aggtcacccc ctttgagaaa ggggacagacaggcctgccc acttgaaatc 71401 tgcggcatct tggatgggag ctgtgcttag caaaaaggtcaacaagttac cggggaagga 71461 ggaagagggt ggattccagg ctctagggtg gtgcaccctgaacctccgcg agggagagag 71521 gagtccacgt ggagtcagca gagctggctg ctggccccaaatctccccta aagagcaaca 71581 gctaacgttc actgtttacg acgctaaacc tactttacaagcattgcatc actgaatcct 71641 cacaaccacc ccttgaggca ggtgttacta tcttcattttacagaggaaa ctgaggccta 71701 ggagtcttat gtgacttacc caaggttgca gtgctggtaaaaggtggagc tagtattcca 71761 cctgcatgca attccaaggc acactcgtcg cccctctgtaacacatgcct ctaatgagac 71821 aggtgaccct ggggcacaag ggcaagtcgc tcccactctcaggaagggca ggaagagcat 71881 ctggctcggg gcattcaagg aagaYgagtg gagattaaagggcacaggaa aagtaaagag 71941 acctgtccag ggaattccac acctgaggca gctcagagaacccaagggag aacagccgag 72001 tctacaagtc ccttccacaa gagggcagac aaggccggatctgatttggg gaataaaggg 72061 aagaaaagag gatctccatg ggctgggatt cggaacaccagggaagagca gactttcacc 72121 atttatcagc atgtttccct ggccgtaccc tacatctgcctgatagcagg tcacacagaa 72181 atcctgatta tatgatgtta cttgacctct ccaaacctatggccatagct atagaatgga 72241 atactagtcc cagccttgcc tactctatag gtagagatgaggcccaaata agaWaataga 72301 tttgcatgtc ctgggtaaac tctacattgt cacacagtaataaggaatta agccattacg 72361 gtaattgcta ctattgcata ggtgtagggt gggttaaagacattgagccc tactgttcag 72421 ctattaaacc ccattaaagg gtgaagtaaa aacacgttggtaaagagttg ggactcagct 72481 ctgcagcaag gggaatggta acgactgggc aaggggtgcagagtctgctc ctgcttggga 72541 cctggatgtg gccagtgctg ggactagagg ataggggagtRggccagccc tgcagcatca 72601 ccagaccaca cagacactag ccaacaccct cccctctccactttagaggc tccagagaca 72661 caatcccagg agcttagcag aatccaaggg agcttgctcaaccaacctaa ttctgccagg 72721 aatatcaaag atgttaaact gacttttggg aaccactctctaagacagac cttgacaaac 72781 tggaatgtNt ttacggaatg cacccagact gagcaaacgactcaacacca ccttctatga 72841 gcaatggctg aaggaactgg agctatttag ccaggagaagagaaggctta tgaggcccat 72901 gaagccacag tgactaccag cagcctctaa gcatggaggtcaggccaagY aaagaaaaaa 72961 acacgtaaca gctagaacct tctaccactg gcactggctgcaggagggag gaggctcact 73021 gtcactggag gtggtaagca gaggctgagt acctgcaaccaaggatgcta cagaaggaat 73081 ttctgaccac atacctgctg ctgtcYagcc actgccttcttgcctgtccc ttccatcttc 73141 ggtgagcctg gccgcccaga cRcaccctct ttgttgccattgctcatcaa caacttcttg 73201 agctccaaat tttcctccct gggatggagg taaacagagaaatcacaatg ctgaccctga 73261 gttaagaaac ctMcagggct ccttaatgct cactatcgcatgagttgcag ctgagcccca 73321 gggagtactg caggatcctg aaacctgccc tcctggaaaaccaaaaaggg catcttcagc 73381 taccttgctc tcaatctgtg gaggtctctg attgtcaatggcagggtttc cccagctgtg 73441 gaatttctat gcacacaaga ccttccggat atatccaaacactgcattaa ataaccctgg 73501 atcacattct agtgagaaag tgacaccctt tccaattatcttcacatttc ttgtgtagac 73561 cacggagacg gtgcgagctt ggtgctaaca ggccctgggcacctctctag ttactcgcca 73621 gtttccctcg ttaacacaga aggtgcaggc tgcaggcccagagccttcaa tgtgcaatga 73681 tccttagcct gaatttatta acatcctttc actttcattgtatttagagt tatcttctat 73741 ttatcacagg taatgctgtt ttccatttac tctagttaaagttgccttta taattttaag 73801 taaaaagatg aatcaaatta aagtaaaatt ctaagtaactaataaggcag gtgatagagg 73861 agatcataaa attagtgatg tggattcagg tctgactaaaatgtttcttc tttctttttt 73921 ttgagatagg gtctcgttct gtcacccagg ctggagtacagtggcccaat ctcggctcac 73981 cacagcctcg actgctccta ctcaagtaat cctcccacctcagccacccc caacctcccc 74041 caccacacac ccccaccctc ttgagtagct gggactacaggcacaccacc acacccagct 74101 aatttttttg gtattttttg ttggggctgg Rggaggggtggtgatgtatt tcaccaggct 74161 ggtctcaaac tcatggactc aaagcaatcc tcccgccttaacgtcccaaa gtgctgggat 74221 tacaggtgtg agccacaacg cccaaccacc acttctttatgttctatttg ccagctgagt 74281 ttccgcttga aaaaccaata agtaaaatga ctttcccaaggtcatacagc agagacttgc 74341 attcttgcct tctaatccgt gacttttttc cccagcttcagtttctgaat ccatggatgg 74401 ggtcaYtagg gctataaaaa ccccaaaagt gcatccttggccatccaaaa gcaaataaat 74461 tacacactga ttcaaaatag ccagtggctc agtagttaaacattacttct gtggccatac 74521 ttacaaagtt tatattcctt ctctttctgt tgattctaataagaaacagg agaggaggga 74581 aaaacagagt ggcaagtcct gtctgtgaca cgggcctttcctatgtgcct gagcccagct 74641 ggtacccacc agatgactca tcacYcaaac tgtccaggaccacagtgcag gcaggaattg 74701 tggccactca caggatggag agcaaggagg cccaagttcYtgttgtaaga ctccaatcaa 74761 gtccctgcct ctccctgggc ctcagtttcc cctctgcacagtgagaggtc tgtctgcatg 74821 atctccaagg gcttgtcaag ctctgacagt caagatcagtgtttcccaag gcgtgatcct 74881 gtgcctacct cgttcataat cacagagact cctggtgaaatgcaggctcc taggtcttgc 74941 ctaagacctg tcaattcaga atcttgcaaa ggaaggaagggaaggcgtat aaacttgtaa 75001 tgcctttccc tgaccctcaa ccctcagtta agtctaagccccctagtcca gtgggggagc 75061 tctcatgttc tcaggaagac agagcaggtt gggatggtgaccagcaggag gcagcaaagt 75121 cctgagtcct ctggacagcc agttcaccgt taacaggggagagaggcaag tgctggagga 75181 ggaaggaagt acaatctatg ctgaccttct tctgaagctctcaaggcact ctatacacct 75241 cccaaggtgg caaaagggcc agagagaaaa taccaaaagagccaaggaga aggatcaaga 75301 gacgtaacct ccaaataggg ctctgggcac agggcctcagagccacggtc ctcaacccag 75361 aaatcagtgc tgcacaccag cYcctcctcc tgcagccccMactcctcacc ccttctctga 75421 ggggcctgaa cttaccgcag cctctcggca gcctgatccYgctgttccag gcccttatcc 75481 aacttggcct tcagagcctc gttctccttc cgaagctgctcacacagggt ctgtggggca 75541 ggggaacaga cagggtgagg gaaaggaggg gcccctcccaggtgcaggct gagctagagg 75601 atggtggggg gcctgtagcc accccagggc ccaactccttatcccctttg cctggcctca 75661 ccccatccct ctctaaggag ctaagccact tggccacccacgtgtcctct cccttgggag 75721 ccaactacgg agaagcctga ccaagtatca gccatggggccttctgctcc tccaagacag 75781 acagctccat cccttctcct tcatccctgc tcccactgccgggaccccta ttccccacca 75841 gcctccccca gctgacttct acYcttcttt gaagtcttagtttaaatccc atctcctcag 75901 agaagtcctc cctgactccc cgatccagct acattaggctaactccgtta tccttgctca 75961 cagccccccg cttttacgct tctcagcacc tgtggcagataaagctaaag aactgttcgt 76021 gctgctgttt tcttaatatc taccccctta cccctaattaattcctgagc tcccggaggg 76081 ctgggacccc taaccatYct cttgttcact gccagatccccagcccccag ctcaaagttt 76141 gtggaaggaa caacatgact gaacagccaa atgagcaaatggacaaatga gcaggcgagt 76201 cctagggggg ctgaaaggca caccaatttg caggtgtgagaggtgcccag tgttgccaag 76261 gggaaaacca gaagacaagg cattcagtcg ggacctcactatgccctcac gccctcacaa 76321 tgtggctgtg acacttggtt cctcctctac aaaagaaagacaactgtttc tctttttttt 76381 tttttttttt ttttttttaa gacagagtct cactctgttgcccaggctgg actgcagtag 76441 catgatctca gctcactgca acctccacct cccaggttcaagagattctc ctgcctcagc 76501 ctcctgagta gctggaacta caggtgtgtg ccaccatgcctggctaattt ttgtattttc 76561 agtacagacg gggtttcacc atgttggcca ggctggtatcaaactcctga cctcaagtga 76621 tccacctgcc tcagtctccc aaagtgctgg gattacaggcatgagccacc acacctagcc 76661 cagggacaac tgtttcttct ctgcctcctc tatagggctgctgagaggga cagatgagac 76741 agtgagagtg gaagtgtcca tgaaccatca gtcacttgcttatgtgcggg gactcatatt 76001 ggaagctctg tgaactgggg gcatggaaga ggggtcgccagactcaggac ctcacaggct 76861 ggggggaagg gaaggtgctg actagcaatg ctagcatacagtgtcagcac agagttgttc 76921 aatctttcca cagatcctca ctgagcacct cacctgtgccaggtacattg ctcggtgttt 76981 ctccatatac tgcctcactg gtgcctcaca acttcctcaaagctaagtaa ctcatctcta 77041 tgttaaaggt agggaaacca ggtaaaatga tacagtctaacaggtgcaca gctgctaaga 77101 ctcatcactg gcggggagta aaactccaat tggcagaccccacactggtg tctctttccc 77161 ctgtgatgaa agatcagaga agggagagag atggtgcccaaaggagggga cagcctttga 77221 gctgggccag gaccagggcc attcactaaa cagccatctgctgccttcca cctcccaggc 77281 ctgttgcagg tggatgcaac catgtgacta gttctggccaacaaaagagg tgtagaaggg 77341 acaagcatca ctcctggact ggcctacgca caattctccagtctcttttt cctggtagca 77401 ggggctgatg tggatgcctc gtattctaaa gggcaccggaaagttgggcc cctgtctccc 77461 tgggactcta aggaactgtg tagagcagaa acaaacccctgccacacaga ataggaggga 77521 aaacttttgt tgtgtaaccc agggagattt cagagttgtttcatttctga ctcacaaagg 77581 agactcttgg gaaggctgaa tggaggacac agccttcaactggaggtggc tggacaatga 77641 gggagatgag gcttagcaaa cagagaacca gctgggcatggtggctcaca ccagtaatcc 77701 cagcactttg ggaggctgag gcaggaggat cacttgagcccaggagttca acaccagtct 77761 gggcaacatg gcaaaaccct gtctctacta aaaataccaaaaaattggcc aggcataagg 77821 gcgcatgcat gtagtctcag ctattcaggg aactgaggtgggagaactga gcccaggagg 77881 tcaaggctgt agtgagctgt ggtcgcacca ctgcactccagcctgggtgg cggagcaaga 77941 ccctgtcccc caacacccct taaaaaaaag aaatcaaaaaattattgtta tgagtccatt 78001 caatgcctag cagagagcct tatgctatac ctgaccttgaatatgtttgt ttagtgaacc 78061 aagaaacaca tcagtgaatg aatgaggagg aactggatcaatacacctcc acccaggcag 78121 taaaaatacc caagatgcca tgtctctggg cgaggcctccagggaacaga atggcctgct 78181 cctagtccct aagggaaggc caggacaagg cctcacttgctgtcctaccc ttgcagtcta 78241 gagagctgtt tccttggagt ctctgggcca atgccctgggtgcaaaccgg ccaatggata 78301 gaatcatcag aggtcacaaa gatcatcacc ttccatccaaggtgctacga gagctatgag 78361 ttcatttgat catttattat tttgtccttt cattcattcaccaagtgtgt atgaagtgcc 78421 acgaactggg aYgcaacagt gaatacaaca gacatggtgcctgcccccct caagcttacc 78481 ttctagcaga gagtataaga aaaaaaagag tataagaaaaaatgtattaa ataaaattat 78541 tacaaactat gataagagct aagaaggaaa taaacaaggaggggactgaa ataaaaaata 78601 acaKaggttg gcaggacccc ttttaagggg cctaggatctatccccatct caaagctgaa 78661 aagtggatgc gcaaagtgct ctcaccccag gcaccttcctaccctgccca cagcctaggc 78721 actgtgccag gctcaccttg ccagggttca gcctggataccttgaagcct cactgacatc 78781 ttaaaggggt tcccttctct cagcctggct ctccaggcctctgcctctcg ttcagcctca 78841 cctcccaaca cacccctccc ctccaggtgg gatgggcatcctccgtgctc ctctaccccc 78901 acacctctcc tcatactgca tctccaacct gattgcccttcatgccctcg gcaaccaagg 78961 tcacgcaagg gggactccca cttctgccat cagcggggcacttgagtgcc tgctgacatg 79021 aatgagacca cagatcagag aatggcctgg gaggagagtctgcagggagt aaggaggctt 79081 caagtcatgg ctctgtcact aaggtggcgg gcactcagatcacgagcagt ccctcagaag 79141 cctccgtttt gccatcttta gaatggtcac caattataactgaacacttg cccgctgcta 79201 aagcccacaa ggctaagacc ccctgcccag tgtggatgcccgtcatgaac tccgaatggg 79261 gacctctctt ccagcatgga ctaggttctg gactgcactgggtgcttcat gtacatggcc 79321 tcatttaatg cagcaatcta accatggagg cacttctagctcatgttact gataggaaca 79381 ttgggggata gagaggataa gtgacttgtc caaggtcatacagcaataaa gggtcactac 79441 tggaaccaag gaccatataa ctccaaggtc tggcctggcctagcaaggaa atctgtccat 79501 tccccacagc acagggaggc tttcaaaagg aattcaataggggaggcagg ttgggatggt 79561 ggtcaagagt atgggctttg gaatgcgaga gatcgaggttccaatcccta cttcaccatt 79621 taccagctgg gtgaccttgg gcaaaaaaag ggctgcttccccacctgtga agtggtgata 79681 atgagggtgc ctaccctggg gggaaagagg cattatgaaaggagttgacc tttgttccct 79741 cccttcccat cccccaaccc tgcacatgag aaataaaggcagggctgctg ggggtatccc 79801 caaccatgac actcgcattg cccatcccgt atttccccatttccttcctc ttcctcatcc 79861 ctccccacaN cttccttatc ctgttcatca ccctgaacaaaaagcccgaa gtctgcccac 79921 aacacagSct tcagaagtcc ccatggtacc agtggattcccaggagcagg aaggtgggaa 79981 gagctcgggg ggaagcacgc ctgcctgtct cgggctgccctccccacgcg cctcacctgc 80041 agaatggagg tgcgctgctc attcttgtgc accttggatgccagtcggtt gaactccagg 80101 gccatgcggc ccaggtgggt gaagagctgg ccgtggtccggctcctcggc acacacactc 80161 agcgtggtct ccaggcgctg caggtgcagc atcaggttgccgtcctcacg ggScaggggg 80221 cccagcRcct ggagagggaa agggcacacg gcccactcttcaccaaggcc tgagtaggcg 80281 ccgccagtcc attctccctc tgcttcctca tttctcatgactcagtttcc ccctgtgacc 80341 ccagctcagc cccgggaaat tctccagtta tgcaggttccctgtctgggg ctgactcaca 80401 gggcagaacc caggactcac aggccactgg tggctccacagcagcggggc caccatcctc 80461 ccacacactg ggccttgcag agtgcctgac agtaagtcacttgtctgctg gacctcagca 80521 tcctcatcta ccaaatgtcc gcacaaccct tccctcccttcaacctcaca gggttcttag 80581 gaggatgaat tgacccctga cagtaacaag cccagaaaagggacatcact gcacagtcag 80641 ccctccatat ccatgggttc cgcatccatg gattcaaccaactgcagatc aaaaatcttc 80701 taaaaaaaca aactggccgg gcgcgatggc tcacgcctgtaatcccacca ctttgggagg 80761 ctgagcgggc agatcacaag gtcaggagat caagacggcctgagtggtga aaccctgtct 80821 ctactaaaaa tacaaaaatt agctgggcat ggtggcacgtgcctgtaatc ccagctactt 80881 gggaggctga ggcaggagaa ctatttgaac cagggagtcggaggttgcag tgagcagaga 80941 tcgcaccaca gcactccagc ctgggcaaca gagcaagattccgtcccaaa acaaacaaac 81001 aaaaaactgt attgaacatg tacagaattt ttttcttgtcattattccct aaacaataca 81061 gtataccaaa atttagcatt tacattgtat taggtatcgtaagtaatcta gagatgattt 81121 aaagtatata ggaggatgtg tataggttat agggaactactacgccattt tacatcaggg 81181 acttgagcat ccatggattt tgggtatcct cagggagttctggaaccact ctcccatgtt 81241 taccaaggga catctgcata tacgtcctgc aacctccgcctcctgggttc aagcgattct 81301 cctgcctcag gctcctgagg agctgggatt acaggtgtgcaccactacac caagctaatt 81361 tttgtatttt tagttgagac ggggtttcac cacaatggccaggctggtct tgaactcctg 81421 acctcaggtg atccacccac ctcggcctcc caaagtactgagattacagg cgtaagccac 81481 catgcctggc ctacacatgt gtcttctggg gaatggcccaagcattcatc agcttctgag 81541 gacccaagac tcaaaacagt ttaaggaacc acagctttagctatttcaga aagtcccaaa 81601 ctagtacagt atgtaagaaa taaagcacac cgtgggccagatagttgact ctttctctct 81661 agtctatctg gaatactcct accaaagaca ttgcctttgttacaccaatt ccactcccaa 81721 ggacctgcag tgttacccta ctgcagacgg cactgggcccagctcccctg ctgggcatct 81781 aaggccctac agccgcaggt cccatccaca gcagcatccagttgggctcc ccactttacc 81841 ctgcctgaac ccacacacac caggtctggt gtctctgcatgtgtcctctc tgtgtcctct 81901 ctgcctctgc aagttctgct catccttccc accctgtccacggctcagct cctccagcag 81961 cctcccggaa acagttatgg gaatcacagg acaKcagagctacagggagc ctcagagatc 82021 aggccgggaa accaagctat ggggaggagg aacttgcccaaagttgccct gtaagtcagt 82081 ggcagaggta taacgagaac tgaggtttcc tgactcgccatccagtgctc tgctgcactg 82141 acctcccact tctctgaact ctggaggaat ggcaatgactataaccMcaa cttggcattc 82201 cctgaacacc tgtgtaatgg cgtcagtgtg ccgcattctaacaagaggaa atgctgctgt 82261 ttcaactctg catccattcc cccactgcct ctttccacaaaggagctgag gtggcagatg 82321 ctgctctatt taaggctcat gcgatgcttc ccatgcctgcgacttctcca gactacaagc 82381 ttcagagcac agggaggctg tgttgtcttg gctcaagtggctcagcagag acaggcacac 82441 tgtagagcta atgaggaact tactacgtca gttaaagttatcggtccaaa gtcagagtga 82501 aacactttct gagcagggga tcttgcctcc atggccagcatctcagagcc ctcagggaca 82561 gaacaggaca aggggacaga aggttccagg cgacagaacataaagagcct ttccaatact 82621 cagagctgcc tgaggcagtg gtgagcctct caacactgaaggcatgctag ttagccagac 82681 agccacctga gggggccYtg cactgggcac tgacaggtacatggggcaac cagtgaacac 82741 aagcatattt gtcagccaaa cctttgtgaa gggaggcagcagggatcaat atatcagtga 82801 agggctagag cacctattgc tcgcataaca acaattaaagRaaaaaactt aaaagtggga 82861 aggaagggag aatggagaag gtagaaattt aagtcgtttaaagaatcttc cagacttttc 82921 acaatggttc ttcaataagt ctgccatttt aagaaagaacagaaggagat atagttagga 82981 aaaaggcagc cagtgttgta aggtgtggaa tgaggatgctgggcagacgc tccagagctg 83041 tacgagagac agagagagag agagagagag agagagagagagagagagag agagagagag 83101 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgcgcgcgcgcgcg cgcatgcgtg 83161 taagtggaaa gttgaacctc cttcctgtgt gctccaagagtctgcacaaa gagYaaatca 83221 cctgtcttac agggccctgg gccttcctaa cccttctttctcccccgggg ttggaagccc 83281 acagacccaa agggctgagt catagtgtgc ggaggtgcctggcccctggc agaaggcaac 83341 agggctggca aagagcccag gctttcacaa cggtcgggtgcccgtgtgct ccaagtcggg 83401 gaagtcacta gcccaggaat ttgcatatgt gagcgagaagcaccctttgc tcctgcccct 83461 cgtgtatccc caagtgactc caggRtacct aasggatctgaacaggttct gtgcctctca 83521 catggtagca agtgacagga cacaaagagg gcagcaggtcaagcccgggg tcctgcccgt 83581 ccactcacca tcgcattggc atggctgctg ctctctggtgaattctgctc ctcaggagtg 83641 accacttcaa attcagagga ggtgccctgt gcagaaaggaagttaggtgc tgcccgagag 83701 aaaaacagct cacccctcct ctgcggccct gtggctgagagcagcaaggg ggacaaaatc 83761 tcttcccatt ttgagtacac agggaccgtt acatatcatctcacatRaga tcacaaagca 83821 ggattctcga caagcctgtc atagccctct gcataatgtgaggtcatatc ccctcgatgg 83881 ccaatcctct tttgtagaag ggagatRtgg gactagctgaccttgaacca gcagcactgt 83941 aggattgcat tttttaaggc cgtgtacttt ttaaatgctttcatgagctc cattatttct 84001 cagggtgacc catggagtgg gtcagaaggt tatatcctctttggaaagat gaggaaacta 84061 cagtatacag ttggttaatg caaaagcagg gattgaacccaagactgcag atccagcatt 84121 cctgccacgg ccccagagct gtctcttagg cccagacgtctctgatcggt ttcccagaac 84181 acagatggca gctgttacgg ccaccaccaa gcatgYcctgtgctggcctt cccaagccag 84241 tcctggagat cccagaggag tgaactgatc tgagaaaagtcccagagatg attcaccaaa 84301 tttggagact atttcaggat tcaaccaaat ccacggtggccaagaaatcc cagatttttt 84361 cacatctttc cgtcattcct agtagaatta ctggtcattcaaccaaccaa gaagccaatg 84421 gcccacctga accagccatt agctctggga tctgggatttccatgtcccg ttaaacctgg 84481 tctgcagcct cccttcatcc cagaaagggg caggatagatgagaggagtc agtgctcagc 84541 ttccagctga ccctcatggc caccaagaac tcacctctggctcttggccc tgccctagaa 84601 agtctccctc ctccatctac attttcccca ttacccgtgtaacctcagaa caggtYcttc 84661 tttccctgca cctgatctgt aggataagga tgctgaactatgcaatccct tgtaggatac 84721 caagcaaaac ttctccagcc aaatctgtag atttttttttttttttatga gagggtctga 84781 ctctgtcacc caggctggag tgtggtggca tcaacatggctcactgcagc cttgacctcc 84841 tgggctcaag cgacccttct gcctcagcca gcctcccaagtagctggaac cacagacata 84901 caccatcaca cctgactact ttttttctta gagagatggagtctcactat cctgcccagg 84961 ctggtctcaa actcctgggt tcaagcaatc ctcctgcctcagcctcccca agtgctggga 85021 ttataggcat gagccactgc gcccagcctg tagaagatagtttctgagcc ctctttgaga 85081 atgtgagctc catgtcacat ctgtctgttt tcctagcactgtgcacactc agtgatactt 85141 aacagtatac aacttagtaa gtgagaagaa ttccttgtacataccagggt cttcctggta 85201 agttttaact atgaccttca tttcaaaatg gggaggctgaggccccagag agtttgcgat 85261 ggacttgccc aatatcaccc agcgagacgg aacacagtttcttgacctca accctctttc 85321 ttcatgttct tgtatctgtc agtaggactt gaggtccatccaggcaacct ccacccatga 85381 ctccaaataa aacttacact ggatggaggc ttctggactggtgctggctt gtcactgggg 85441 catgcagRgg ctgtgggaga tgctgtgaca tttgagtcctttcctggaga atcaagaaag 85501 cacagatgaa ctgactggga aggggagaag cccaggtaccaccctctcaa ggacagccct 85561 tgacaaaagg gttctcagac aggattcccc actcgagtgtgggagaatgg gaggtggtct 85621 catcagggcc aggtagtctg agaaaatggc ctgccctggagtgacggggc agagcctgag 85681 cagagagacc atgctccctg ggcttactgg gaaaatcccaagttggctgc tgctccttgc 85741 tgccctgaac aggcctcaat gataaagaca atgatttacaatttgaaaag tctttatgta 85801 tctattatcc cagccattct cataaaagcc ccatatccccattttaaagg tcaggacact 85861 gaggcttggg aaggtcatca Mcttgcccac agccacatagccagcaactg caaactcagg 85921 actgagactt ggccctccct ccctgacttc ctgcagtccttgctgcaggg agctccttga 85981 aggtagcttc atttctcact ctgagagtgg acctggcaccaatcaggaac tcaggaacat 86041 tgctgaatag acaagcagag gctggaaatg cccactgactgcagcacagt ggactctgag 86101 accatcactt tcctccattt gtccattcag caaatgcaaccaccagccaa tagaaggctg 86161 tcacattggc ctgggccaga taccatacag tagagctgcagcaggaggca tctgagtcca 86221 gctgaggtgc ggatcatttc catcctggtc ccagaccttaccctggactt ggagacatgc 86281 cagagtaatg cacactctgt acgcatggtg atgcctctaccatacatggg acttcaaccc 86341 cgagccacag tcaccctgtc ctttaatggg cagcacacgtaaccttacat ctgaaatacg 86401 ctgcagcagg ctaacaggca agcccagctt cttccctgggcctcagcttc cctaattgta 86461 aaatggacgc gcagggcacg accagggagc ttccaactcatatgccagct ccaagtgaca 86521 gggagtagct gcttggagYg cgatgcctgg cacacagtagctgctcatta aatattaaat 86581 atgctgataa agaccccaag gccacaggac tgcaatgtagtcaactgttg tatctattac 86641 tcatgtctgc tggggcctca ggagtgctga gtcactggctatttttaaca agattgtttt 86701 gaagtccctt ccaaatctga ccttctgggg gatgtggcagccaaaacatg aatggaaggg 86761 tagcctgtga cctaaggata aacgagagaa tgtggRttttggcataagaa gggagttcac 86821 tgtgaaggat ttgggcagtt tggggtacag ggagagtcagaggtacccag actcctctta 86881 tacctgtgag ctcagccagg gggtcgaagg agcccaaggagggagaaggt ggtgggagca 86941 gctcgttgtc cttcactagc tcctctgcct tctgccggagcctggtcgct tccatctggg 87001 actcttccaa aagctcccct agagttattg gggaagaggaagcaaaagca tttactYggc 87061 tcagtgtgct gcttctcccc gtcccaggcc cctaaccgtgctgccgcctg gcttctgagg 87121 ctccaggccc tggacttcac tcccaccgtt gctctgtgggccaagtggga caaactccat 87181 gctggtccac cataagaaac aggctagagc aaggacgagggcccagcaat cgggggacac 87241 aggagcggaa ggaatctatg agaaactcct cggctcccccaacccccagc attctctttc 87301 tgccccactc ccttcacacc cagccagggc actccacagaaagccagggg ctcccccacc 87361 ctacgggcta cccacagcct gagccaccag ggcaaagggctgcctgcagg gcggagacct 87421 tggataattc caaccagccc tctaggcctc cattttccttctcctacaag tggagatggg 87481 gaaaccactc ctccctcccc agtcggccat cccaagcaaggatctgccaa gcaaggagaa 87541 agcattttaa aaatcagggg gctatgctcc cctctaaaacatgagactag agaagggcac 87601 ggcaaggcaa aggaagagtt tcaggagtgt ggaagaaagagagccccctc tggatgggga 87661 aatcaatgaa agccttgtct ggaggagaag ggggcacagcaactgggtgc tgatgcacag 87721 ggcccgtcgg tcgtgtggag ggaggtgggg ttgttctaaagtggggatgg cttgagcaag 87781 agccaggaga agagaactga gggacctacc acctccttgcttcaagccaa gctctgctcc 87841 ctgctccaac acctaggaac gcgtgcccca gttgcctggtcatgctggct ctccccatct 87901 ctcgtgcccc agaaagagga gRccactcta gttccatggccattaactgg gccttccagc 87961 cccctttccc ttagcccacg cctccctctt ggacctgagtcctgtcatcc tggtttccgg 88021 gtgctgagga atcagggttg ggaatactgt caccatgctacatggtgcat agagacagct 88081 gtgaggcagg agccagcRct gggccaggag ggacaggggatgacttgggc agaggcatat 88141 gccagcttca ttctccactg ctagtagagg gactggcatcacagtctgca gggaggggcg 88201 ctcgcaggga ggggacaggg tctgctttac ctaacatctttatcccttgc attttttcct 88261 tcagccggga attctccttc actaggcgct caaaagctgcggatgcctct cctgagggca 88321 cgctgccccc agggtcgtag atccggtacg gtcctctcccttccatgagg gtagctcagc 88381 ccctgccgtg gtgcccgcct ggctgtaagg acaacagcagcatatcagca ggctggccag 88441 gccatggggg catcctttgc agagaagctc tagtctgtcaatgccccaga aggcagtccc 88501 ccactttaag ctggtctgac catattatag taaagcaggacagtcacctc cttttatctg 88561 gacgctctgc ctctattaat acaaccttag aatggcattagtttctctta gcagcctcaa 88621 tgcactgttg gcttgtttgg aacaggagtc caaataaacagtagggtgca atgtgggata 88681 caagaaagaa cctggacttg gaaaaaaaaa aatctgagttcatgtcctgt ctgtgaggtc 88741 cagaagaaca ggaatcccat atgttttacc catcattgcatcctcagagc ctaccacagt 88801 attagctatg aattttgccc aataaacact tactcaattaatggtctagg cattaattta 88861 ctatgggatt atgggcagtc ccttcccctc tctgagggagggaaatgaaa gtcttaagtt 88921 aatttagtaa tctttaaagt gaaatgcttg taccctaagggataagcaag tcaacccagt 88981 ccaaagttcc atttatatgt gtatcatctt ctttaaaaaaagaaaagaaa aaaacaccct 89041 gactaatatt taatacactg tcaccctcct ccgtgagtctcatatcatat atggtctgtc 89101 ccttgcagtt ctaaggaaca gaagaatttt tcatcaaaccactgtctatt tgagcaccaa 89161 ggtttttttt tagtaatctg ttcagtttca tctgctttatgtcactgaaa ccagttggaa 89221 aggtgatttt agaatcctaa ctctgtggct tagtagagaagctctctgtc cattcactca 89281 tccattcaac aattacttat tgagcatcga ctccgtgcKaggcaccagtc tgggtgctga 89341 ggaattatca gcaaataaaa cagacaaaaa tccttcatggagcttatagt ctaggggtga 89401 aagcagagga ggatggtggg gaacacaata tttcaggtaatcaaaaagtc tatgcacaaa 89461 aggccccaag cataaggagc tattttagcc aagacctgaaagaggtgagg gaactagcca 89521 tgaagacatc tgaaggaaga acattctttg cagagagaacagtaagggca gaggccctaa 89581 ggctgaaaca ccgagccagc ctccagccct ccaaatagctctccagcatg aaagggccaa 89641 ggccttcact ggggacctca gaagggtttg ggggctcRgcaccttcctcc caaggtcacc 89701 acccccaaga caaaaactgt ttcatcagga atcctcaaagaccctcccaa gtccacacac 89761 catgctccgt gggtccttgg cgctgccagg tgactacgaccataccaccc tgctcccaga 89821 atgtaaatga aagatcagag tgagaaagga ttaacagcaacaccaccatg cttgaatcgc 89881 ctccttgtca ggcactgcat taaatgtttc agatatacaatcttggagga gctgcacagt 89941 aactctaaca tgcatactaa tctcatctgc attttacaagggagaaactg aggctcaggg 90001 cggttaagta actcagccac gatgaMccgg ccRcaataagagccaagatg caaagtcagc 90061 cctgcctgct gtgactctga cccccaccat actcacacagccaggacaca gagScagagg 90121 ggcagacagg gagttcaaat ctctttgtag ggcagcaccatgctatcagg tcacctctgg 90181 gcagtttccc ttgccctgca gcataagaac caataaggacttgtccaaga gcgtccatag 90241 gatggaatgg gccagaaaga aaaacaaagt tctgatgagaaggacccctt ctcccaaaga 90301 ctgacagata caagatgaat acaccRtgtg ggaggcacccacgccctgct gagcctgcga 90361 ggcaaaggac agctacccac actcccatca caagtcatcacccggcttag gggtgacttg 90421 tttttaacct gaaaggtcag tgttctacag catagcacaaagYgcagatg tttccaccga 90481 actaagaaac agtgtggggg ctgcacttag cactggaactggcacatagt aaagtactca 90541 aaaatgtYgg cagacattgg gggtccttcc cctctgcttgcctctgtcct cacgaggctt 90601 gccacctggt acagcatgtg tctgactggt gcgggcattgcccatctggc tgggaacttc 90661 ctgaggcagg agcgtacttt gcacatcttt gtattcctgtcgggagcaca ggagggtggg 90721 ccttccttga gagctgcttg gagttcaaga acagtgaaacagagggctac aaggggcctg 90781 gagccacctt gcatgacaaR ggcagcagtg tcctggttcttgtccaccat tatgcatKca 90841 ccctaaatgg aactcacagt caacaccaca tgaaggcatggggaatttct tactcaatgt 90901 ctgccttgtc ctgtagatta gaggctgcat gaggacagggagcaagtcca tcctgtgtgc 90961 cagggtatct tcagagtggc ccagagtagg gcctcggtaaaccttgaatt cagagaagtc 91021 agactgaagg gaacaagccc ttgaccccga ccccgcctcctgcaaggcca tgtccaacat 91081 cccagagaaa gcctaaccct gacccactga ccagacgcacttggacgccc agacacttct 91141 agaatccagg ctgggccagc catgctccct cagagaggcccagatgtaag gcgaagggac 91201 aaagcaggat gcaggatgtg ggaactgaaa cccaccccacgtcctccaaa acacgcaaca 91261 ctcagtttct cttggcgctg gggagtcaac gggaaaggggttcatgggaa acaaaaagtg 91321 ggcagacaga gctatgctcc cctactttag cctcagggggcagagcttgg ggagaaagaa 91381 aggcagtggg cagaggagaS gcaggagaaa tatcctattgtgggaaaacc ctctctggag 91441 atcagaggag ccacccctgg agggcctgga cttctggaggaaagaaagac aaagagggaa 91501 acagggaatt tccaagttct gctgacctca tgcccagatggtcagcggca gtccctggat 91561 gaccactggg cagacgggaa ctctccctct gatcaatgaggtttttataa cttctcccag 91621 agagaggact cattctcagg atgacagagc tggaggggactctccaaggg caatgaggcg 91681 aatcctgcat tatcaggtgg gagagagatg cacccagaaacactcaatgg atgaggggct 91741 gcattcgaac atgacgatgg catgcactcc cctctgctgtccacaaacca cgaggatgtt 91801 ttcaaagggg aaaaggaagc actgaccact cccctccatgtggcagtatc tggagttcag 91861 agcccaggag tcctcgggca tgagctcatc tgattctcactcacggcaca ggcagtgcag 91921 caaggcaagg ccctgagcag ggaaggccaa ggtcacaaaggagtcaggat ggagctggga 91981 ccagaagatc cctgctctcc ctccaggctg cccacagcccatcctccatt tcccttcctg 92041 cccagcaatc ccatggcaca ccacaggccc ccaccacatcttctcRgagt ctctttccac 92101 ctggcctggt tccacaaact acaaatcagc aggtgctccaccagcagtgg gactccagcc 92161 ccgcttgccc aaaccagaaa cagctggtgg tatagaagcagccagcgcag actttcttcc 92221 aaaacaagac ctcagccagc ggggcctcca cagcctgccccagaggggcc ctggggctgc 92281 ccgctggaac cagaaaggcc cagtttgggg gcagaaaggggcccagacag acggcaggtc 92341 aggcaacaac cacagaccgt gaagatggaa gtgatgctgctgaaaccgaa ggagccaccc 92401 actagcaggc aagcacttgt gggtggcagg gaggcagtagtccccagggc tcaactctca 92461 gccttacccc agaaagagag gggtatgata aagactacattcagaattaa atttacattc 92521 tagcttttgc tctgaaatgc tttggtctgt cagattatcttcccattagc agtgttctgg 92581 tggctgggta ggggggtggt cccagacccc ccaaatgatattcacttgca tagccactaa 92641 catttgcaaa atacttcata gtgtcccaag cattgtttttccatctgatt atcacaacag 92701 ccctgggaag aaggcagggc tgatacctgc ctgatggataaggaaactga agtccagctt 92761 ggggacttaa tcagcccaat ttaatgcctt tggtcactggctgcatcctg gggccagcta 92821 tccctgcaac cctggagcag gtgatgccct ccctggcccagagggaaaag tgtaagcaaa 92881 tctctggtac agggaggtgg aaggtcatcg aggccaacatctacctcccc agcagaggtg 92941 agggctgcca gggatgctgg caggtgggct ggcagagggggtatagggaa ggggtctcca 93001 ggaaagacgg cacagaacag aacgtcagag aggtctattctagtgtctac tctgctgtgt 93061 atggaaccat gggctcattc cctcacatgc aaggggaggagcatgggcca cacagaaacc 93121 gggtgatggc taaagctggc ctcaggatca agaagacccaagtctcagga gctagcagca 93181 agctgaggaa agagagagag ccagcaccat ggaaacatcgctgccttgga ggcaggaaga 93241 aaggtccaca cagggaaagt gctcaaggcc aaccttgcgaacacgaggcc tgagaggggg 93301 cctcacctgc actagcctca gctctgtcca ccctgtgggctgtgaaaccc tggagcagcc 93361 actcaccttt cctgagcctc aggtgcctca tctgtaaagcaggagtaata atagaaccta 93421 cctgatagaa gatcaaagaa attaaatgaa acaatatgtgtatagttgga agggaattct 93481 cactttctac tttaggcaat acttcctgat tatctgatttaacaagaaac acatctttgt 93541 tttataataa gatttccaaa ataataagag tatacagtaactagctcagc atttgacaca 93601 cagcaggtgc ttaataaaag gctgaggcaa ccccacaactgtgcccagct tatctaaggg 93661 ccaaggcttt ccccatttgt tctctccttt aatgggattttaggctcttc cgtcagctca 93721 gtaagctagg ttctggcctc cacttattct ctagtgacctgcatatcacc atttaactgt 93781 ggttaagcag ggtgcctgga cacaccatag ctagtgaacatgtagctata agaagccctt 93841 gaaccacgat gccacactca ccaacaactg ggaatctgagcctcatttcc tctgctgtaa 93901 agggcaatat cctgcaattc cacccaagga tgaaaggaagtgagaYgcca tgatatcaca 93961 ttgcagacat aaacaaaaaa ctgtccacat atttgtgttatgatgacctc cagtcctccc 94021 caaccaattt ccaggctggg gaaactgcag cctcaggtccatccatcaaa ggccgggttg 94081 gatttttcag gcagtgaaac tactctgttc aatactgtaatggtggacac ctgccattcc 94141 acatttgtcc aaacccatag aacatacacc accaagagggaacactgatg taaaccatgg 94201 actccgggtg acagtggtgt caatgtagat tcatcaactgtaaaaaatct accactctgg 94261 tggaggatgt tgataatggg ggaagctatg cctgttaggggtgggggggg cgcagtatat 94321 gtaaaatctc tgtaccttct tctcaatttt gctgtgaacctaaaactgct ctataaaaaa 94381 taaggtcttt aatttaaaag agaaaaggac caggctgggctctcctgatg gctgtcaggt 94441 tcacacatga gtgaggacac agcacatact tgagacagtgtgtattcaag gaccgcaccc 94501 tggcatcctc accaacaagg agcccagact taactcctccatccttgcaa tcaactgagc 94561 aacaatgagg ctgcagacac agagtctcat atagtaacagctggggcgcc agaactgtgg 94621 gatctagtca aggtacacta gggcgaacat cttaaccctcactaggcctc agttacctaa 94681 aaagagggag ctgcaaaaat agtaataaca tggggggttgtgcgggataa tcattaaggc 94741 cccttccagt tctaacattt caggatctga aatttattagcactactttc tttaaccttc 94801 taaccttaag aaggctccaa tccccagctc caatcccttctaaggcccag gtggatttcc 94861 ctaacccatc agtcctccca taagagcttt cataggtgctatcccctccc cgagggcagt 94921 cagagaccct cagctgacat ccgcagccct gtgccaggtcctcccctcct gcctaacttg 94981 taccaggctc tggccaagcc tgatctgtgt tcctcaacatggcacacatc ttcccatgtc 95041 cttcatcctc ccctcccgag tcacccacaa agaaggccccactgtctcca ctcagcctcc 95101 gaggcccaga ttacacacca cccgctaaac acctgagccagaattcacac tgcaaaagag 95161 gactggagag cacctaacta agctggagag agggtgacagttcgttaggc gctaccgtat 95221 gccaggtgct gggccgcatc atgacgcaca tgacctcattttcttcttgc cacacagata 95281 tggaaattat catccacact ttccagcaag gaaaccagtcgacgcagcta catggatgag 95341 gaagcagact cagagaagtY aagtcatttg cctgagaatacacagctggt tagtggtaga 95401 acaaataatg cagcctcaat ctgtcagatt ccaacatcagtgccctttcc actgctctac 95461 actgcctgga gaggcggcag gctgaagctc agaggtgggaaaccagacct actttccagt 95521 tctccagaac gtgccccaag ttcctagccc ctgcctttatatgtctctag ctttatatgc 95581 aaccaggtgg caatctgccc cacatcacct gcagtcaacagggtttagaa tctcaggctg 95641 gaagggagct cagaggacat tccatcctgt gcttacccagtcctctgagg agcccaggga 95701 ctccagctgg ccaactttag caatccagat ctcaccaacttcctccccaa aacaattcct 95761 ggggcagcca gcactggcta cagttaggct gctcccagttctgtttccac tggcccttct 95821 ctcccctctg tggtcccctg gaataaggaa caagatgcccatcttcagca tgacagccag 95881 ccctgaggac atttggaacc tgcagccttc tctatactgttccacatagg gactgccccc 95941 cctccagtcc cccaccaacc ctctacccca cactatctctagttggttct agcactggac 96001 ccaacattcc aggcatggtt tgcctgacca aatgcaggctttcacctcca cccttttagg 96061 tagtatattc ctattgaggg agcctgggta tacatttgcttttcttacag ctgagtcacc 96121 tactgaagaa tgtcctttct caatgacaac aacctgcatgtatgcctaac tggtgagaac 96181 aaagttctca tgtagaaacg tgtgcttgag ggctgggtgcggtggctcat gcctgtaatt 96241 ccagcacttt gggaggccga ggtgggcgga tcacctgaggtcaggagttc gagatcagcc 96301 tggccaacat ggcaaaaccc agtctctact aaaaatacaaaaattagcca ggtgtggtgg 96361 cgggtgcctg tagtcccagc tactggggag gctgaggtaggagaatcgct tgaacttggg 96421 aggtggagat tgcagtaagc cgagatcacg ccacagcactccagcctggg agacagagtg 96481 ggactccatc tcaaaagaaa aaaaaaaaaa aaaaggggtgtttgaggagg tgtgagttac 96541 ctaagggggc agaagtaact gattgattca gtaatMccctatctaggaat ctagagatct 96601 ttgcatcagt acacagaagc tggacaaaaa tatgcactgccacatgcttt gtaatagaca 96661 tttttgctat tcttgaatgt ccatcaagaa gggactggttaaacaaacca ccattcattc 96721 Ratggaatac tatacagcca tcaaaaagaa tggggtagattgcttggcaa tgatacagaa 96781 agatgcccat aaaatacaaa gcaaagtgca gatttatatgtgtaacagga tgctattttt 96841 ttcaaaaaca cattgtgtgt gtgtgtgtat acatacggaatcttaaatgt gcatagaaaa 96901 aggcctggaa gcccagtgct ttgggaggcc aaggtgggaggattgcttga ggccaggggt 96961 tcaagaccag cctgggcaaa aagcaagaat agattctctattaaaaaaaa acttttttac 97021 attagcagag catggtggca catgcctgga gtcccagctactcggaaagt tgaggtggga 97081 ggatcacttg agcccagcag ttcaggctac agtgagctatgatcatgcca ctgaactcca 97141 gcctgagtga cagactaata ctctgtctct taaaaaaaagaaaagaagaa aagaaaaaag 97201 tctcaaagga tacataccaa actgctacta gtagactgctctacagaagg acctcggtaa 97261 ggaactaaaa ccaaaagaaa cttttatttt acctatgctattttaactct ttacatggga 97321 aatctagtcc tgttcttata caattataaa attagtttttaaatacagta gggacagagg 97381 aaggggaaag gaaggagaga gaagacaact gtcattttttacattatatc ttttgtactg 97441 cttgagtttt ggggggtttt tccccaataa tcatgtgctatttggtaatt ttttttaaag 97501 ggaaatttta aaatattttt ctaaaaaatt taagtgagctgagtgcagtg gtgcatgcct 97561 gtagtcccag ctactccaga gacagacgca ggaggatggcctgaaaccag agtttatcta 97621 accctgtaac tcccctgcct ccaagaggta agatgccaattttggggaat tcctcttata 97681 caaaatatgg tggaaagagg gtttcacaga ttcaaatcctggctatgatc ccttctcgaa 97741 tgaccctggg taggcctcac tttccugtt tctccctatggaaatgttat gaggaccaag 97801 taggaaccat agagttctct gtaaacctta aaatgctacccagacaggag cagtaatggc 97861 tgccaaggag ctaggaagga aaaggggctg gacaggggctgaggtactca gatgaaagcc 97921 aaagatgcct tgtgttaaca acaaaaagct gaaagctacctaaaatgaag agaaaaatga 97981 ataaatacat tgtggcaatt tcctatcagg aaatactatacagcagtgaa aaaagaatga 98041 gccacagctg gagatacgaa catgaatgaa tctgggtctcactctgttgc ccaggctgga 98101 gtgcagtggc atgatctcag ttcactgcaa cttccacctcccagcccaag tcatcctccc 98161 acctLagcct ctcaagtagc tggaactaca ggcatgcgccaccacaccca gctaattttt 98221 gtattgtttg cagagatggg ttttggccat gttgcccaagctggtctcaa actcctgggc 98281 tcaagagatc cacctgcctg ctctgcctcc caaagWgccagtaaggcatg agtcaccacg 98341 ctcagcctaa ataacatttt taaacataaa aaaatactatgtattattta gggatgcaca 98401 cattaaaaaa aaaactttaa agaaatgcaa gaaaaaaaccccactaaatt cagaacactt 98461 gtttcttgtt ggtggctgat gaaggagaaa tttcaaStgattggaaatgt gttatttctt 98521 aaaccagatg agtgagcatg taggtgcctg cgtgttaccctcggtatctg agagactcct 98581 tcaatttttc ttttttcttt ttactgtatt gtttgctttttttctgcttc aacttttatt 98641 ttagattcgg ggggtacatg tgcaggtttg ctacctgggtatactgcgtg atttgggtag 98701 gggtgatccc atcacctggg tactgagcat agttcccaacagtttttcaa ctctgcctcc 98761 ctccctcccc actctagcag tccccagtgt ctactgttgccatctttatg tccatgagga 98821 cctgatattt agctccactt ataagtgaga aaatgcagcatttggttttc tgttcctgtg 98881 ttaatgtgct taggacaata ttttctaaaa gtctatgttgccagtttcct gaggaccaga 98941 tcacgcagga gttgcacctg cagagacaga agtgggctggagagggacca gggctcctaa 99001 gtctgctgct gaagctgact atagcgaagg caaaggacaagtggcctgaa ggccaacaga 99061 gagcctggcc aatgctctgc ccagtgggtg gagactgagccaaaggccct tgactcatag 99121 ggcagtccca tacctgccct ggcctctcag catgactccttggtgacaca actcccacag 99181 cacccacaca cctacaccca cctaccccac acacacacccctgcctgggc tgcccggttt 99241 gtccactgtg taggtgctca aggacgcaga gctcctgcctctcaaggaac agcctcaggg 99301 tgaggtccct gggagagaag agttcagctc tgcccagagcagccctcaga caaacagcca 99361 gctgctgctc accagtcatc cRttccaatc tgaagagtgactgtcctggg ccagtcatgg 99421 gaaaatcatc aagaacttcc cRcaatggtc atggtggccgccaaggtctt tctctggggg 99481 ttgggagctg tgctccccca caaaacaagg ggacccagctgccctgtgcc ttcctgtggc 99541 aactctctgg aggatgcctg gataccctcc ctacagacggccagggatag gaggggcagg 99601 cactgcaggc atgtgggggc atagcagcaa gaatctaggagggcaggcac agcaaggcct 99661 ggcccagagg gaaaccagga gctgccagag ccaaagagagggtgacccaa tcattggttg 99721 ttatagagtt tttaagactt ggaagagaat gccctgagatgccaNagagt gaaacatttt 99781 cattctaaag ctgggaaacc aaagtcggca aaggcgaacagacgtgccca cagccacatg 99841 ggacagcagt ggcacagaag aaatgcacac ctcgtcctctggtggccact gtgcactctc 99901 ttaccacacc atgctactgc tcaacctctc cccctccctcacagtggtca ggtaagcaat 99961 gagggaaaat gatggaggtg agaaaggaag aacccgggct

Following is a CETP coding nucleotide sequence (cDNA, SEQ ID NO: 6).>gi|4557442|ref|NM_(—)000078.1| Homo sapiens cholesteryl ester transferprotein, plasma (CETP), mRNA. 1 gtgaatctct ggggccagga agaccctgctgcccggaaga gcctcatgtt ccgtgggggc 61 tgggcggaca tacatatacg ggctccaggctgaacggctc gggccactta cacaccactg 121 cctgataacc atgctggctg ccacagtcctgaccctggcc ctgctgggca atgcccatgc 181 ctgctccaaa ggcacctcgc acgaggcaggcatcgtgtgc cgcatcacca agcctgccct 241 cctggtgttg aaccacgaga ctgccaaggtgatccagacc gccttccagc gagccagcta 301 cccagatatc acgggcgaga aggccatgatgctccttggc caagtcaagt atgggttgca 361 caacatccag atcagccact tgtccatcgccagcagccag gtggagctgg tggaagccaa 421 gtccattgat gtctccattc agaacgtgtctgtggtcttc aaggggaccc tgaagtatgg 481 ctacaccact gcctggtggc tgggtattgatcagtccatt gacttcgaga tcgactctgc 541 cattgacctc cagatcaaca cacagctgacctgtgactct ggtagagtgc ggaccgatgc 601 ccctgactgc tacctgtctt tccataagctgctcctgcat ctccaagggg agcgagagcc 661 tgggtggatc aagcagctgt tcacaaatttcatctccttc accctgaagc tggtcctgaa 721 gggacagatc tgcaaagaga tcaacgtcatctctaacatc atggccgatt ttgtccagac 781 aagggctgcc agcatccttt cagatggagacattggggtg gacatttccc tgacaggtga 841 tcccgtcatc acagcctcct acctggagtcccatcacaag ggtcatttca tctacaagaa 901 tgtctcagag gacctccccc tccccaccttctcgcccaca ctgctggggg actcccgcat 961 gctgtacttc tggttctctg agcgagtcttccactcgctg gccaaggtag ctttccagga 1021 tggccgcctc atgctcagcc tgatgggagacgagttcaag gcagtgctgg agacctgggg 1081 cttcaacacc aaccaggaaa tcttccaagaggttgtcggc ggcttcccca gccaggccca 1141 agtcaccgtc cactgcctca agatgcccaagatctcctgc caaaacaagg gagtcgtggt 1201 caattcttca gtgatggtga aattcctctttccacgccca gaccagcaac attctgtagc 1261 ttacacattt gaagaggata tcgtgactaccgtccaggcc tcctattcta agaaaaagct 1321 cttcttaagc ctcttggatt tccagattacaccaaagact gtttccaact tgactgagag 1381 cagctccgag tccatccaga gcttcctgcagtcaatgatc accgctgtgg gcatccctga 1441 ggtcatgtct cggctcgagg tagtgtttacagccctcatg aacagcaaag gcgtgagcct 1501 cttcgacatc atcaaccctg agattatcactcgagatggc ttcctgctgc tgcagatgga 1561 ctttggcttc cctgagcacc tgctggtggatttcctccag agcttgagct agaagtctcc 1621 aaggaggtcg ggatggggct tgtagcagaaggcaagcacc aggctcacag ctggaaccct 1681 ggtgtctcct ccagcgtggt ggaagttgggttaggagtac ggagatggag attggctccc 1741 aactcctccc tatcctaaag gcccactggcattaaagtgc tgtatccaag CDS: 131-1612 base pairs Signal peptide: 131-181base pairs Mature peptide: 182-1609 base pairs PolyA signal: 1771-1776base pairs

Following is a second PROL4 cDNA sequence (SEQ IDNO:7). >gi|6005801|ref|NM007244.1| Homo sapiens proline rich 4(lacrimal) (PRR4), mRNA 1 cagagcctcc ttcaagatgc tgctggtcct gctctcagtgtccttctgg ctctgagctc 61 agctcagagc acagataatg atgtgaacta tgaagactttactttcacca taccagatgt 121 agaggactca agtcagagac cagatcaggg accccagagacctcctcctg aaggactcct 181 acctagaccc cctggtgata gtggtaacca agatgatggtcctcagcaga gaccaccaaa 241 accaggaggc catcaccgcc atcctccccc acctccttttcaaaatcagc aacgaccacc 301 ccaacgagga caccgtcaac tctctctacc ccgatttccttctgtcagcc tgcaggaagc 361 atcatcattc ttccggaggg acagaccagc aagacatccccaggagcaac cactctggta 421 atctagaatt cagtggcaga aaataaataa gaagataacttcattcagaa agccatgaca 481 ttgaaataat gtggtcataa ctctttc CDS: 17 . . .421 base pairs PolyA signal: 442 . . . 447 base pairs

Following is a GRID2 cDNA sequence (SEQ ID NO: 8).>gi|4557632|ref|NM_(—)001510.1| Homo sapiens glutamate receptor,ionotropic, delta 2 (GRID2), mRNA. 1 atggaagttt tccccttgct cttggttttgtccgtctggt ggtctcgaac ctgggactcg 61 gcgaatgcgg attcgatcat tcacatcggagcaatttttg atgaatctgc caaaaaggat 121 gatgaggtat ttcgcactgc ggttggtgaccttaaccaga atgaagagat cttacagact 181 gagaaaatca cattttcagt gacgtttgttgatggcaaca accctttcca agcagttcaa 241 gaagcctgtg aacttatgaa tcaaggcatcttggccctgg tcagctccat tggctgcacg 301 tcagcaggat ccctccagtc tttggcagacgccatgcata tcccccacct cttcattcag 361 cgctcaacag ctgggacccc aaggagtggctgtggactca cccggagcaa caggaatgat 421 gactacactc tctcagttcg cccacctgtctacttgcatg atgttatcct aagagtggtc 481 acagagtatg cctggcagaa attcattatattctatgata gtgaatacga tatccgtgga 541 atacaggagt tcttggacaa agtctctcagcagggaatgg atgttgcact tcagaaggta 601 gaaaacaaca tcaataaaat gattaccactctctttgaca ccatgagaat agaagaactg 661 aatcgctatc gagacactct taggcgagcgatccttgtta tgaatcctgc tacagccaaa 721 tccttcatta ctgaggttgt ggagactaatttggttgctt ttgactgtca ctggatcatt 781 ataaatgagg aaataaacga tgtggacgtacaggaacttg taagaaggtc aattggaagg 841 ttaacgatta ttcggcagac atttccaattccccagaaca taagtcagcg gtgtttccgt 901 ggcaaccatc gaatatcttc aacattgtgtgatccaaagg atccatttgc tcagaatatg 961 gagatttcca acctttacat atatgacacggtgcttctgc ttgctaatgc ttttcataag 1021 aagctggagg accgaaagtg gcacagcatggcaagtctgt catgtatcag aaagaactca 1081 aagccctggc agggtgggcg ctccatgttggagaccatca agaagggtgg agttagtggg 1141 ttgactggag agctagaatt tggagaaaatggaggcaatc ccaatgtcca ctttgaaatc 1201 cttggaacca actatggaga agagcttggcagaggtgttc gaaaacttgg gtgctggaat 1261 cctgtcacag gtctgaatgg gtcactgactgacaagaaat tggagaataa catgcgtgga 1321 gtggttctac gtgtagtaac tgttctggaagaaccttttg tgatggtctc tgaaaatgtc 1381 ttgtgtaagc cgaagaaata ccagggcttctccattgatg ttttggatgc cttatctaac 1441 tacctgggtt ttaactacga aatttacgtagcaccggatc acaaatacgg aagcccacaa 1501 gaagatggga catggaatgg cttggtaggagaacttgtct ttaagagagc cgacataggg 1561 atttctgctt taaccatcac tccagatcgtgaaaatgtgg tggactttac gacacgttac 1621 atggactact cagtgggggt actacttcgaagggctgaaa agacagtgga tatgtttgcc 1681 tgtcttgcac catttgatct ctctctatgggcttgcattg ctggcacagt ccttctggtg 1741 ggtctactgg tctacctctt gaactggcttaatcccccac gattacaaat gggatcaatg 1801 acgtctacta ctctctacaa ctccatgtggtttgtgtatg gatcttttgt acaacaaggc 1861 ggggaagtcc cgtacacgac tctggctacccgaatgatga tgggggcttg gtggctattt 1921 gctttgattg ttatctcatc ttacacggcaaacctcgctg ctttcctcac tattacacgc 1981 attgaaagtt ccatccagtc tctccaggacctttccaagc aaacagaaat cccttatggc 2041 acagtcctag actctgcggt atatgagcatgtccgcatga aaggactgaa tccttttgag 2101 agggacagca tgtattccca aatgtggcggatgatcaacc gaagcaatgg atcggagaac 2161 aatgttctgg agtcccaggc aggcattcaaaaggtaaaat atggaaatta tgctttcgta 2221 tgggatgcag ctgtattgga atatgtggctatctatgacc cagattgttc cttttacacc 2281 attggaaata ctgttgctga tcggggatatggaattgcat tacaacatgg cagtccttac 2341 cgagatgttt tttcacaaag gatcctggagcttcagcaga atggtgacat ggacatcctg 2401 aagcacaaat ggtggcctaa gaatggccagtgtgacctgt actcgtcagt ggacacaaag 2461 cagaaaggag gcgccctgga cataaagagctttgcagggg tcttttgtat cctggctgct 2521 ggaattgtcc tctcctgctt catagccatgctggagacgt ggtggaacaa gaggaaaggc 2581 tcccgggttc catcaaaaga ggatgacaaggaaattgacc tggagcacct ccatagacgt 2641 gtaaatagct tgtgcacaga tgacgacagcccccataaac agttttccac ctcgtcaatt 2701 gatttgaccc ctctggacat tgacactttgccaacacgac aagcactgga gcaaatcagt 2761 gatttcagga acactcatat taccacaacaacctttatcc cagagcagat ccagactctt 2821 agccgcacac tgtcagctaa agctgcttctggtttcactt ttggcaacgt gcctgagcac 2881 cgaactggcc cttttaggca cagggcacctaatgggggct ttttcaggag tcctataaaa 2941 acaatgtcat ctattcctta tcaaccaactcctaccctgg ggctcaatct gggtaatgat 3001 ccagaccgag gcacctccat atga

Following is a PDE4D cDNA sequence (SEQ ID NO: 9).>gi|4636198|ref|_(—)006203.3| Homo sapiens phosphodiesterase 4D,cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) (PDE4D),mRNA. 1 ggaattcatc tgtaaaaatc actacatgta acgtaggaga caagaaaaatattaatgaca 61 gaagatctgc gaacatgatg cacgtgaata attttccctt tagaaggcattcctggatat 121 gttttgatgt ggacaatggc acatctgcgg gacggagtcc cttggatcccatgaccagcc 181 caggatccgg gctaattctc caagcaaatt ttgtccacag tcaacgacgggagtccttcc 241 tgtatcgatc cgacagcgat tatgacctct ctccaaagtc tatgtcccggaactcctcca 301 ttgccagtga tatacacgga gatgacttga ttgtgactcc atttgctcaggtcttggcca 361 gtctgcgaac tgtacgaaac aactttgctg cattaactaa tttgcaagatcgagcaccta 421 gcaaaagatc acccatgtgc aaccaaccat ccatcaacaa agccaccataacagaggagg 481 cctaccagaa actggccagc gagaccctgg aggagctgga ctggtgtctggaccagctag 541 agaccctaca gaccaggcac tccgtcagtg agatggcctc caacaagtttaaaaggatgc 601 ttaatcggga gctcacccat ctctctgaaa tgagtcggtc tggaaatcaagtgtcagagt 661 ttatatcaaa cacattctta gataagcaac atgaagtgga aattccttctccaactcaga 721 aggaaaagga gaaaaagaaa agaccaatgt ctcagatcag tggagtcaagaaattgatgc 781 acagctctag tctgactaat tcaagtatcc caaggtttgg agttaaaactgaacaagaag 841 atgtccttgc caaggaacta gaagatgtga acaaatgggg tcttcatgttttcagaatag 901 cagagttgtc tggtaaccgg cccttgactg ttatcatgca caccatttttcaggaacggg 961 atttattaaa aacatttaaa attccagtag atactttaat tacatatcttatgactctcg 1021 aagaccatta ccatgctgat gtggcctatc acaacaatat ccatgctgcagatgttgtcc 1081 agtctactca tgtgctatta tctacacctg ctttggaggc tgtgtttacagatttggaga 1141 ttcttgcagc aatttttgcc agtgcaatac atgatgtaga tcatcctggtgtgtccaatc 1201 aatttctgat caatacaaac tctgaacttg ccttgatgta caatgattcctcagtcttag 1261 agaaccatca tttggctgtg ggctttaaat tgcttcagga agaaaactgtgacattttcc 1321 agaatttgac caaaaaacaa agacaatctt taaggaaaat ggtcattgacatcgtacttg 1381 caacagatat gtcaaaacac atgaatctac tggctgattt gaagactatggttgaaacta 1441 agaaagtgac aagctctgga gttcttcttc ttgataatta ttccgataggattcaggttc 1501 ttcagaatat ggtgcactgt gcagatctga gcaacccaac aaagcctctccagctgtacc 1561 gccagtggac ggaccggata atggaggagt tcttccgcca aggagaccgagagagggaac 1621 gtggcatgga gataagcccc atgtgtgaca agcacaatgc ttccgtggaaaaatcacagg 1681 tgggcttcat agactatatt gttcatcccc tctgggagac atgggcagacctcgtccacc 1741 ctgacgccca ggatattttg gacactttgg aggacaatcg tgaatggtaccagagcacaa 1801 tccctcagag cccctctcct gcacctgatg acccagagga gggccggcagggtcaaactg 1861 agaaattcca gtttgaacta actttagagg aagatggtga gtcagacacggaaaaggaca 1921 gtggcagtca agtggaagaa gacactagct gcagtgactc caagactctttgtactcaag 1981 actcagagtc tactgaaatt ccccttgatg aacaggttga agaggaggcagtaggggaag 2041 aagaggaaag ccagcctgaa gcctgtgtca tagatgatcg ttctcctgacacgtaacagt 2101 gcaaaaactt tcatgccttt ttttttttta agtagaaaaa ttgtttccaaagtgcatgtc 2161 acatgccaca accacggtca cacctcactg tcatctgcca ggacgtttgttgaacaaaac 2221 tgaccttgac tactcagtcc agcgctcagg aatatcgtaa ccagttttttcacctccatg 2281 tcatccgagc aaggtggaca tcttcacgaa cagcgttttt aacaagatttcagcttggta 2341 gagctgacaa agcagataaa atctactcca aattattttc aagagagtgtgactcatcag 2401 gcagcccaaa agtttattgg acttggggtt tctattcctt tttatttgtttgcaatattt 2461 tcagaagaaa ggcattgcac agagtgaact taatggacga agcaacaaatatgtcaagaa 2521 caggacatag cacgaatctg ttaccagtag gaggaggatg agccacagaaattgcataat 2581 tttctaattt caagtcttcc tgatacatga ctgaatagtg tggttcagtgagctgcactg 2641 acctctacat tttgtatgat atgtaaaaca gattttttgt agagcttacttttattatta 2701 aatgtattga ggtattatat ttaaaaaaaa ctatgttcag aacttcatctgccactggtt 2761 atttttttct aaggagtaac ttgcaagttt tcagtacaaa tctgtgctacactggataaa 2821 aatctaattt atgaatttta cttgcacctt atagttcata gcaattaactgatttgtagt 2881 gattcattgt ttgttttata taccaatgac ttccatattt taaaagagaaaaacaacttt 2941 atgttgcagg aaaccctttt tgtaagtctt tattatttac tttgcattttgtttcactct 3001 ttccagataa gcagagttgc tcttcaccag tgtttttctt catgtgcaaagtgactattt 3061 gttctataat acttttatgt gtgttatatc aaatgtgtct taagcttcatgcaaactcag 3121 tcatcagttc gtgttgtctg aagcaagtgg gaaatatata aatacccagtagctaaaatg 3181 gtcagtcttt tttagatgtt ttcctactta gtatctccta ataacgttttgctgtgtcac 3241 tagatgttca tttcacaagt gcatgtcttt ctaataatcc acacatttcatgctctaata 3301 atccacacat ttcatgctca tttttattgt ttttacagcc agttatagcaagaaaaaggt 3361 ttttcccctt gtgctgcttt ataatttagc gtgtgtctga accttatccatgtttgctag 3421 atgaggtctt gtcaaatata tcactaccat tgtcaccggt gaaaagaaacaggtagttaa 3481 gttagggtta acattcattt caaccacgag gttgtatatc atgactagcttttactcttg 3541 gtttacagag aaaagttaaa caaccaacta ggcagttttt aagaatattaacaatatatt 3601 aacaaacacc aatacaacta atcctatttg gttttaatga tttcaccatgggattaagaa 3661 ctatatcagg aacatccctg agaaacggct ttaagtgtag caactactcttccttaatgg 3721 acagccacat aacgtgtagg aagtccttta tcacttatcc tcgatccataagcatatctt 3781 gcagagggga actacttctt taaacacatg gagggaaaga agatgatgccactggcacca 3841 gagggttagt actgtgatgc atcctaaaat atttattata ttggtaaaaattctggttaa 3901 ataaaaaatt agagatcact cttggctgat ttcagcacca ggaactgtattacagtttta 3961 gagattaatt cctagtgttt acctgattat agcagttggc atcatggggcatttaattct 4021 gactttatcc ccacgtcagc cttaataaag tcttctttac cttctctatgaagactttaa 4081 agcccaaata atcatttttc acattgatat tcaagaattg agatagatagaagccaaagt 4141 gggtatctga caagtggaaa atcaaacgtt taagaagaat tacaactctgaaaagcattt 4201 atatgtggaa cttctcaagg agcctcctgg ggactggaaa gtaagtcatcagccaggcaa 4261 atgactcatg ctgaagagag tccccatttc agtcccctga gatctagctgatgcttagat 4321 cctttgaaat aaaaattatg tctttataac tctgatcttt tacataaagcagaagaggaa 4381 tcaactagtt aattgcaagg tttctactct gtttcctctg taaagatcagatggtaatct 4441 ttcaaataag aaaaaaataa agacgtatgt ttgaccaagt agtttcacaagaatatttgg 4501 gaacttgttt cttttaattt tatttgtccc tgagtgaagt ctagaaagaaaggtaaagag 4561 tctagagttt attcctcttt ccaaaacatt ctcattcctc tcctccctacacttagtatt 4621 tcccccacag agtgcctaga atcttaataa tgaataaaat aaaaagcagcaatatgtcat 4681 taacaaatcc agacctgaaa gggtaaaggg tttataactg cactaataaagagaggctct 4741 ttttttttct tccagtttgt tggtttttaa tggtaccgtg ttgtaaagatacccactaat 4801 ggacaatcaa attgcagaaa aggctcaata tccaagagac agggactaatgcactgtaca 4861 atctgcttat ccttgccctt ctctcttgcc aaagtgtgct tcagaaatatatactgcttt 4921 aaaaaagaat aaaagaatat ccttttacaa gtggctttac atttcctaaaatgccataag 4981 aaaatgcaat atctgggtac tgtatgggga aaaaaatgtc caagtttgtgtaaaaccagt 5041 gcatttcagc ttgcaagtta ctgaacacaa taatgctgtt ttaattttgttttatatcag 5101 ttaaaattca caataatgta gatagaacaa attacagaca aggaaagaaaaaacttgaat 5161 gaaatggatt ttacagaaag ctttatgata atttttgaat gcattatttattttttgtgc 5221 catgcatttt ttttctcacc aaatgacctt acctgtaata cagtcttgtttgtctgttta 5281 caaccatgta tttattgcaa tgtacatact gtaatgttaa ttgtaaattatctgttctta 5341 ttaaaacatc atcccatgat ggggtggtgt tgatatattt ggaaactcttggtgagagaa 5401 tgaatggtgt gtatacatac tctgtacatt tttcttttct cctgtaatatagtcttgtca 5461 ccttagagct tgtttatgga agattcaaga aaactataaa atacttaaagatatataaat 5521 ttaaaaaaac atagctgcag gtctttggtc ccagggctgt gccttaactttaaccaatat 5581 tttcttctgt tttgctgcat ttgaaaggta acagtggagc tagggctgggcattttacat 5641 ccaggctttt aattgattag aattctgcca ataggtggat tttacaaaaccacagacaac 5701 ctctgaaaga ttctgagacc cttttgagac agaagctctt aagtacttcttgccagggag 5761 cagcactgca tgtgtgatgg ttgtttgcca tctgttgatc aggaactacttcagctactt 5821 gcatttgatt atttcctttt tttttttttt taactcggaa acacaactgggggaat

Following is a GPX3 cDNA sequence (SEQ ID NO: 10).>gi|6006000|ref|NM_(—)002084.2| Homo sapiens glutathione peroxidase 3(plasma) (GPX3), mRNA. 1 agccaaaaga ggaagggacc ggcctcccac gtccacagggacctgacttc cacctctctg 61 cccagatttg cttatgtcac tgtcgccccg ggacggggaggtggggagct gagggcaagt 121 cgcgcccgcc cctgaaatcc cagccgccta gcgattggctgcaagggtct cggcttggcc 181 gcggattaat cacacccgag ggcttgaaag gtggctgggagcgccggaca cctcagacgg 241 acggtggcca gggatcaggc agcggctcag gcgaccctgagtgtgccccc accccgccat 301 ggcccggctg ctgcaggcgt cctgcctgct ttccctgctcctggccggct tcgtctcgca 361 gagccgggga caagagaagt cgaagatgga ctgccatggtggcataagtg gcaccattta 421 cgagtacgga gccctcacca ttgatgggga ggagtacatccccttcaagc agtatgctgg 481 caaatacgtc ctctttgtca acgtggccag ctactgaggcctgacgggcc agtacattga 541 actgaatgca ctacaggaag agcttgcacc attcggtctggtcattctgg gctttccctg 601 caaccaattt ggaaaacagg aaccaggaga gaactcagagatccttccta ccctcaagta 661 tgtccgacca ggtggaggct ttgtccctaa tttccagctctttgagaaag gggatgtcaa 721 tggagagaaa gagcagaaat tctacacttt cctaaagaactcctgtcctc ccacctcgga 781 gctcctgggt acatctgacc gcctcttctg ggaacccatgaaggttcacg acatccgctg 841 gaactttgag aagttcctgg tggggccaga tggtatacccatcatgcgct ggcaccaccg 901 gaccacggtc agcaacgtca agatggacat cctgtcctacatgaggcggc aggcagccct 961 gggggtcaag aggaagtaac tgaaggccgt ctcatcccatgtccaccatg taggggaggg 1021 actttgttca ggaagaaatc cgtgtctcca accacactatctacccatca cagacccctt 1081 tcctatcact caaggcccca gcctggcaca aatggatgcatacagttctg tgtactgcca 1141 ggcatgtggg tgtgggtgca atgtgggtgt ttacacacatgcctacaggt atgcgtgatt 1201 gtgtgtgtgt gcatgggtgt acagccacgt gtctacctatgtgtctttct gggaatgtgt 1261 accatctgtg tgcctgcagc tgtgtagtgc tggacagtgacaaccctttc tctccagttc 1321 tccactccaa tgataatagt tcacttatac ctaaacccaaaggaaaaacc agctctaggt 1381 ccaattgttc tgctctaact gatacctcaa ccttggggccagcatctccc actgcctcca 1441 aatattagta actatgactg acgtccccag aagtttctgggtctaccaca ctccccaacc 1501 ccccactcct acttcctgaa gggccctccc aaggctacatccccacccca cagttctccc 1561 tgagagagat caacctccct gagatcaacc aaggcagatgtgacagcaag ggccacggac 1621 cccatggcag gggtggcgtc ttcatgaggg aggggcccaaagcccttgtg ggcggacctc 1681 ccctgagcct gtctgagggg ccagccctta gtgcattcaggctaaggccc ctgggcaggg 1741 atgccacccc tgctccttcg gaggacgtgc cctcacccctcactggtcca ctggcttgag 1801 actcaccccg tctgcccagt aaaagccttt ctgcagcaaaaaaaaaaaaa aaaaaa CDS: 299-979 base pairs Signal peptide: 299-358 basepairs Mature peptide: 359-976 base pairs PolyA signal: 1838 base pairs

Following is a CETP amino acid sequence (SEQ ID NO:11). >gi|4557443|ref_NP900069.1| cholesteryl ester transfer protein,plasma precursor [Homo sapiens]MLAATVLTLALLGNAHACSKGTSHEAGIVCRITKPALLVLNHETAKVIQTAFQRASYPDITGEKAMMLLGQVKYGLHNIQISHLSIASSQVELVEAKSIDVSIQNVSVVFKGTLKYGYTTAWWLGIDQSIDFEIDSAIDLQINTQLTCDSGRVRTDAPDCYLSFHKLLLHLQGEREPGWIKQLFTNFISFTLKLVLKGQICKEINVISNIMADFVQTRAASILSDGDIGVDISLTGDPVITASYLESHHKGHFIYKNVSEDLPLPTFSPTLLGDSRMLYFWFSERVFHSLAKVAFQDGRLMLSLMGDEFKAVLETWGFNTNQEIFQEVVGGFPSQAQVTVHCLKMPKISCQNKGVVVNSSVMVKFLFPRPDQQHSVAYTFEEDIVTTVQASYSKKKLFLSLLDFQITPKTVSNLTESSSESIQSFLQSMITAVGIPEVMSRLEVVFTALMNSKGVSLFDIINPEIITRDGFLLLQMDFGFPEHLLVDFLQSLS Protein: 1 . . . 493 aminoacids Signal peptide: 1-17 amino acids Mature peptide: 18-493 aminoacids

Following is a second PROL4 amino acid sequence (SEQ ID NO: 12).>gi|6005802|ref|NP_(—)009175.1| proline rich 4 (lacrimal) [Homosapiens]. MLLVLLSVVLLAISSAQSTDNDVNYEDFTFTIPDVEDSSQRPDQGPQRPPPEGLLPRPPGDSGNQDDGPQQRPPKPGGHHRHPPPPPFQNQQRPPQRGHRQLSLPRFPSVSLQEASSFFRRDRPARHPQEQPLW Protein: 1 . . . 134 amino acidsSignal peptide: 1-16 amino acids Mature peptide: 17-134 amino acids

Following is a GRID2 amino acid sequence (SEQ ID NO: 13).>gi|4557633|ref|NP_(—)001501.1| glutamate receptor, ionotropic, delta 2[Homo sapiens]. MEVFPLLLVLSVWWSRTWDSANADSIIHIGAIFDESAKKDDEVFRTAVGDLNQNEEILQTEKITFSVTFVDGNNPFQAVQEACELMNQGILALVSSIGCTSAGSLQSLADAMHIPHLFIQRSTAGTPRSGCGLTRSNRNDDYTLSVRPPVYLHDVILRVVTEYAWQKFIIFYDSEYDIRGIQEFLDKVSQQGMDVALQKVENNINKMITTLFDTMRIEELNRYRDTLRRAILVMNPATAKSFITEVVETNLVAFDCHWIIINEEINDVDVQELVRRSIGRLTIIRQTFPIPQNISQRCFRGNHRISSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLEDRKWHSMASLSCIRKNSKPWQGGRSMLETIKKGGVSGLTGELEFGENGGNPNVHFEILGTNYGEELGRGVRKLGCWNPVTGLNGSLTDKKLENNMRGVVLRVVTVLEEPFVMVSENVLCKPKKYQGFSIDVLDALSNYLGFNYEIYVAPDHKYGSPQEDGTWNGLVGELVFKRADIGISALTITPDRENVVDFTTRYMDYSVGVLLRRAEKTVDMFACLAPFDLSLWACIAGTVLLVGLLVYLLNWLNPPRLQMGSMTSTTLYNSMWFVYGSFVQQGGEVPYTTLATRMMMGAWWLFALIVISSYTANLAAFLTITRIESSIQSLQDLSKQTEIPYGTVLDSAVYEHVRMKGLNPFERDSMYSQMWRMINRSNGSENNVLESQAGIQKVKYGNYAFVWDAAVLEYVAIYDPDCSFYTIGNTVADRGYGIALQHGSPYRDVFSQRILELQQNGDMDILKHKWWPKNGQCDLYSSVDTKQKGGALDIKSFAGVFCILAAGIVLSCFIAMIETWWNKRKGSRVPSKEDDKEIDLEHLHRRVNSLCTDDDSPHKQFSTSSIDLTPLDIDTLPTRQALEQISDFRNTHITTTTFIPEQIQTLSRTLSAKAASGFTFGNVPEHRTGPFRHRAPNGGFFRSPIKTMSSIPYQPTPTLGLNLGND PDRGTSI

Following is a PDE4D amino acid sequence (SEQ ID NO: 14).>gi|32306513|ref|NP_(—)006194.2| cAMP-specific phosphodiesterase 4D[Homo sapiens] MMHVNNFPFRRHSWICFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT

Following is a GPX3 amino acid sequence (SEQ ID NO: IS).>gi|6006001|ref|NP_(—)002075.2| plasma glutathione peroxidase 3precursor [Homo sapiens]MARLLQASCLLSLLLAGFVSQSRGQEKSKMDCHGGISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYUGLTGQYIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDILSYMRRQAALGVKRK

Modifications may be made to the foregoing without departing from thebasic aspects of the invention. Although the invention has beendescribed in substantial detail with reference to one or more specificembodiments, those of skill in the art will recognize that changes maybe made to the embodiments specifically disclosed in this application,yet these modifications and improvements are within the scope and spiritof the invention, as set forth in the claims which follow. Also,citation of the above publications or documents is not intended as anadmission that any of the foregoing is pertinent prior art, nor does itconstitute any admission as to the contents or date of thesepublications or documents. The content of each patent, patentapplication and other publication and document referenced isincorporated herein by reference in its entirety, including drawings,tables and cited documents.

1. A method for identifying a subject at risk of low bone mineraldensity (BMD), which comprises detecting the presence or absence of oneor more polymorphic variations associated with low BMD in a nucleic acidsample from a subject, wherein the one or more polymorphic variationsare detected in a nucleotide sequence in SEQ ID NOs:1-5, a substantiallyidentical sequence thereof or a fragment of the foregoing; whereby thepresence of the polymorphic variation is indicative of the subject beingat risk of low BMD.
 2. The method of claim 1, which further comprisesobtaining the nucleic acid sample from the subject.
 3. The method ofclaim 1, wherein the one or more polymorphic variations are detected atone or more positions selected from the group consisting of rs7500979,rs2217332, rs8044804, rs2270835, rs2133783, rs247609, rs952440,rs881598, rs2291955, rs2518054, rs866038, rs1436425, rs173537, rs247611,rs166017, rs173538, rs193694, rs7205692, rs8048746, rs247618, rs183130,rs6499863, rs4783961, rs3816117, rs711752, rs708272, rs1864163,rs4369653, rs1864165, rs891141, rs891143, rs7205804, rs5885, rs1532625,rs1532624, rs289712, rs7499892, rs5883, rs289714, rs158480, rs289717,rs4344729, rs289718, rs289719, rs2033254, rs4784744, rs291044,rs8053613, rs5881, rs5880, rs7198026, rs5882, rs8045701, rs289741,rs1801706, rs289742, rs289743, rs289746, rs172337, rs289747, rs1566439,rs7205459, rs289749, rs289751, rs8059220, rs8058353, rs289735, rs289737,rs291042, rs1875236, rs821466, rs821465, rs4275846, rs289707, rs821463,rs289706, rs1167741, rs2052880, rs1167742, rs1183256, rs1651665,rs1651666, rs4784751, rs1651667, rs8052091, rs1684574, rs1684575,rs1672865, rs821470, rs1549669, rs291040 and rs289754.
 4. The method ofclaim 1, wherein the one or more polymorphic variations are detected atone or more positions selected from the group consisting of rs166017,rs193694, rs7205804, rs1801706, rs7205459 and rs821465.
 5. The method ofclaim 1, wherein the one or more polymorphic variations are detected ina region spanning positions 14,328 to 68,805 in SEQ ID NO:
 1. 6. Themethod of claim 1, wherein the one or more polymorphic variations aredetected at one or more positions selected from the group consisting ofrs523051, rs693620, rs2588349, rs2588350, rs619381, rs3759252,rs3759251, rs2418107, rs7303054, rs1838345, rs620878, rs2537817,rs1548803, rs667123, rs1838346, rs2159903, rs3944035, rs3741845,rs2110096, rs759055, rs589377, rs7960194, rs7978242, rs601051,rs4262797, rs2215714, rs1373434, rs2215715, rs612456, rs612808,rs689118, rs597468, rs592864, rs640372, rs7966559, rs654834, rs4763216,rs668521, rs669503, rs3906864, rs3906863, rs7957888, rs9300230,rs7306214, rs763839, rs2418105, rs666841, rs3851578, rs7138797,rs7295252, rs2418106, rs7299578, rs621112, rs3863320, rs1373432,rs1047699, rs1063193, rs2232959, rs2227296, rs1548804, rs2232958,rs2232957, rs2232956, rs1972571, rs3759250, rs3759249, rs1541525,rs2098248, rs2900550, rs7302130, rs4763583, rs4360778, rs1607695,rs1607694, rs2192139, rs7978300, rs7397871, rs4763217, rs2159900,rs10772370, rs7398682, rs2900551, rs2900552, rs2418214, rs2418215,rs965243, rs1117548, rs1520225, rs1520226, rs1520227, rs971919,rs2159901, rs2159902, rs2110099, rs7314847, rs7296003, rs4281556,rs4763219, rs3851579, rs3851580, rs1049119, rs2298866, rs2298865,rs2298864, rs2298863, rs3180393, rs2070837, rs7956204, rs2418216,rs3741844, rs4262798, rs2418217, rs2418218, rs7137492, rs2110100,rs1013312, rs4579993, rs1013313, rs7397106, rs2215716, rs2192140,rs4763589, rs1468697, rs2070837, rs3180393 and rs2298865.
 7. The methodof claim 1, wherein the one or more polymorphic variations are detectedat one or more positions selected from the group consisting ofrs2588350, rs619381, rs620878, rs759055, rs4262797, rs612808, rs3906863,rs7957888, rs763839, rs2418105, rs666841, rs3851578, rs7299578,rs621112, rs1047699, rs1548804, rs2232956, rs1520227 and rs2215716. 8.The method of claim 1, wherein the one or more polymorphic variationsare detected in a region spanning positions 2,424 to 93,715 in SEQ IDNO:
 2. 9. The method of claim 1, wherein the one or more polymorphicvariations are detected at one or more positions selected from the groupconsisting of rs1433661, rs1485009, rs7681947, rs1816432, rs1485018,rs1485017, rs7438397, rs6834311, rs1368717, rs1017391, rs2870701,rs7679839, rs1385404, rs1368716, rs4693316, rs1905707, rs1905708,rs1905709, rs3912442, rs2082553, rs6831638, rs5860329, rs2870702,rs2870703, rs1948016, rs6835836, rs1994253, rs1905710, rs1485019,rs978191, rs1385405, rs7694361, rs1905711, rs1905734, rs1485012,rs1485013, rs4692981, rs7670552, rs7670932, rs7688091, rs7440540,rs2171000, rs2870704, rs7655758, rs7661436, rs7662289, rs7667044,rs7691929, rs5860330, rs901013, rs901012, rs901011, rs1948018,rs2870705, rs1948017, rs1905733, rs1385408, rs1385409, rs1385410,rs1485026, rs1485027, rs2904483, rs1385406, rs1905732, rs2046418,rs2200377, rs1905731, rs1905730, rs975713, rs6820985, rs7670441,rs6810794, rs7676623, rs1154861, rs1032125, rs1485022, rs1485024,rs3913651, rs4693319, rs1872383, rs2200376, rs7668090, rs7692930,rs967096, rs6822249, rs6532405, rs1017897, rs7672674, rs7694568,rs2904484, rs7340830, rs1485033, rs2870706, rs1905729, rs4693320,rs6848749, rs6532406, rs6532407, rs1905728, rs6819866, rs1905727,rs7674069, rs1905724, rs1905723, rs1485020 and rs6814101.
 10. The methodof claim 1, wherein the one or more polymorphic variations are detectedat one or more positions selected from the group consisting ofrs1433661, rs7679839, rs1368716, rs1905707, rs1905708, rs1994253,rs1485019, rs1905734, rs1485012, rs7670552, rs7691929, rs1948018,rs1948017, rs1485024, rs7694568, rs4693320, rs6848749, rs6532406,rs6532407 and rs6819866.
 11. The method of claim 1, wherein the one ormore polymorphic variations are detected in a region spanning positions206 to 90,969 in SEQ ID NO:
 3. 12. The method of claim 1, wherein theone or more polymorphic variations are detected at one or more positionsselected from the group consisting of rs6886495, rs6450498, rs1472456,rs4700315, rs4700316, rs7714708, rs7710479, rs2968013, rs2968014,rs2968015, rs1391648, rs2055297, rs2055296, rs3989138, rs4700317,rs2036220, rs7727206, rs7723432, rs1546221, rs4479801, rs4395595,rs4395596, rs4699932, rs2936201, rs7356672, rs2936200, rs1909296,rs7703131, rs7445308, rs3087748, rs4321723, rs2968016, rs5868151,rs1874858, rs1874857, rs7712922, rs4631140, rs4469166, rs1078369,rs1078368, rs2968006, rs2968005, rs2936190, rs2409613, rs4415048,rs2968004, rs2968003, rs2968002, rs2936191, rs1498610, rs6874662,rs3060393, rs7729722, rs7733884, rs7714489, rs7735570, rs2936193,rs2291851, rs2291852, rs1498602, rs1995166, rs1498603, rs1498604,rs1498605, rs1948651, rs4699934, rs4700319, rs2279737, rs7720361,rs7706419, rs1006431, rs1353747, rs1498606, rs1353748, rs1553113,rs2968012, rs2968011, rs1498608, rs2936189, rs1498609, rs2968019,rs6891238, rs2968010, rs2968009, rs2936203, rs1498601, rs1498600,rs1498599, rs2936202, rs7730070, rs6450501, rs6450502, rs6889456,rs6894618, rs7706044, rs7707541, rs7712076, rs6892860, rs6867053,rs7737269, rs6864156, rs950447, rs2936196, rs7719347, rs1391649,rs1391650, rs1391651, rs1353749, rs10682149, rs5868153, rs1363882,rs2409626, rs2968018, rs954740, rs986067, rs6869400, and rs5010782. 13.The method of claim 1, wherein the one or more polymorphic variationsare detected at one or more positions selected from the group consistingof rs7714708, rs1498602, rs4699934, rs1006431, rs1353747, rs1498608,rs1498609, rs2968010, rs2936202 and rs1391649.
 14. The method of claim1, wherein the one or more polymorphic variations are detected in aregion spanning positions 1,599 to 82,591 in SEQ ID NO:
 4. 15. Themethod of claim 1, wherein the one or more polymorphic variations aredetected at one or more positions selected from the group consisting ofrs1478398, rs1478397, rs1160114, rs1160113, rs1382323, rs1160112,rs7709870, rs7710643, rs7730467, rs6579829, rs6579830, rs6579831,rs6896232, rs1351131, rs1038074, rs1478396, rs6880512, rs4958858,rs4958431, rs4958432, rs6898463, rs4958859, rs4130064, rs4130065,rs4133119, rs4958860, rs4958861, rs4437356, rs4958868, rs1478400,rs6889375, rs1600159, rs6875892, rs4608909, rs2345000, rs4516840,rs2054440, rs707141, rs707142, rs841236, rs707143, rs707144, rs6869405,rs707145, rs707146, rs707148, rs707150, rs5872184, rs3763015, rs2042235,rs3763013, rs2042236, rs1946234, rs1946235, rs1946236, rs8177402,rs8177403, rs8177404, rs8177405, rs8177406, rs8177407, rs8177408,rs8177409, rs6888961, rs8177410, rs8177411, rs8177412, rs8177413,rs870407, rs870406, rs6873202, rs8177414, rs8177415, rs3805435,rs8177416, rs3792799, rs3792798, rs3828599, rs8177417, rs3792797,rs8177418, rs8177419, rs8177420, rs8177421, rs4958872, rs3792796,rs8177422, rs8177423, rs4958434, rs8177424, rs8177425, rs8177426,rs8177427, rs8177429, rs6889737, rs3792795, rs8177430, rs8177431,rs4958873, rs8177432, rs8177433, rs8177434, rs8177435, rs3763011,rs8177436, rs8177437, rs4958874, rs8177439, rs8177440, rs8177441,rs8177442, rs8177443, rs869975, rs869976, rs8177444, rs8177445,rs7721469, rs8177446, rs7704191, rs8177447, rs11548, rs2230303,rs7722386, rs8177448, rs8177449, rs2070593, rs8177450, rs8177451,rs8177452, rs8177453, rs8177454, rs3763010, rs8177455, rs8177456,rs736775, rs2277940, rs8177458, rs8177834, rs3924, rs2233312, rs2233311,rs2233310, rs2233309, rs4958875, rs2233308, rs2233307, rs2233306,rs2233305, rs2233304, rs2233303, rs2233302, rs2287719, rs2287720,rs7727034, rs7727250, rs7709800, rs3840312, rs2287721, rs6875293,rs3805434, rs2080982, rs2080983, rs2287722, rs2233301, rs2233300,rs4958876, rs2233299, rs2233298, rs2287723, rs2161359, rs7734456,rs4292439, rs4958878, rs6862024, rs3834819, rs2233297, rs2233296,rs2233295, rs2233294, rs7713028, rs7713223, rs7713567, rs888989,rs2233293, rs3749657, rs2233292, rs2112635, rs871269, rs3792794,rs6579837, rs3805433, rs5872186, rs2233291, rs2233290, rs2233289,rs4958435, rs4958880, rs1422673, rs2042234, rs3805432, rs3805431,rs2233288, rs2233287, rs3815720, rs3792792, rs3792791, rs2303018,rs3792790, rs4958436, rs2233286, rs2233285, rs7732451, rs2233284,rs1422674, rs3792789, rs4562032, rs6865077, rs1559126, rs3792788,rs1559127, rs3792786, rs6880110, rs6861227, rs3805430, rs1862364,rs4958881, rs3792785, rs6869605, rs6870205, rs4246047, rs4958882,rs3792784, rs3792783 and rs5872188.
 16. The method of claim 1, whereinthe one or more polymorphic variations are detected at one or morepositions selected from the group consisting of rs1478398, rs1160114,rs1160113, rs1160112, rs4958858, rs4958431, rs6898463, rs4958859,rs4958860, rs4608909, rs707144, rs2042235, rs3763013, rs2042236,rs8177404, rs8177426, rs8177427, rs8177429, rs3792795, rs4958873,rs8177437, rs869975, rs8177447, rs11548, rs2277940, rs8177834,rs2233311, rs2233302, rs7727034, rs7727250, rs3805434, rs7734456,rs7713028, rs7713223, rs888989, rs3792794, rs4958880, rs1422673,rs3805432 and rs4958436.
 17. The method of claim 1, wherein the one ormore polymorphic variations are detected in a region spanning positions231 to 86,539 in SEQ ID NO:
 5. 18. The method of claim 1, wherein theone or more polymorphic variations are detected at one or more positionsin linkage disequilibrium with one or more positions selected from thegrout consisting of rs7500979, rs2217332, rs8044804, rs2270835,rs2133783, rs247609, rs952440, rs881598, rs2291955, rs2518054, rs866038,rs1436425, rs173537, rs247611, rs166017, rs173538, rs193694, rs7205692,rs8048746, rs247618, rs183130, rs6499863, rs4783961, rs3816117,rs711752, rs708272, rs1864163, rs4369653, rs1864165, rs891141, rs891143,rs7205804, rs5885, rs1532625, rs1532624, rs289712, rs7499892, rs5883,rs289714, rs158480, rs289717, rs4344729, rs289718, rs289719, rs2033254,rs4784744, rs291044, rs8053613, rs5881, rs5880, rs7198026, rs5882,rs8045701, rs289741, rs1801706, rs289742, rs289743, rs289746, rs172337,rs289747, rs1566439, rs7205459, rs289749, rs289751, rs8059220,rs8058353, rs289735, rs289737, rs291042, rs1875236, rs821466, rs821465,rs4275846, rs289707, rs821463, rs289706, rs1167741, rs2052880,rs1167742, rs1183256, rs1651665, rs1651666, rs4784751, rs1651667,rs8052091, rs1684574, rs1684575, rs1672865, rs821470, rs1549669,rs291040 and rs289754.
 19. The method of claim 1, wherein detecting thepresence or absence of the one or more polymorphic variations comprises:hybridizing an oligonucleotide to the nucleic acid sample, wherein theoligonucleotide is complementary to a nucleotide sequence in the nucleicacid and hybridizes to a region adjacent to the polymorphic variation;extending the oligonucleotide in the presence of one or morenucleotides, yielding extension products; and detecting the presence orabsence of a polymorphic variation in the extension products.
 20. Themethod of claim 1, wherein the subject is a human. 21-55. (canceled)